MST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MST1
Entrez Gene ID	853640
Full Name	threonine--tRNA ligase MST1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179760.1	NP_012727.1	threonine--tRNA ligase MST1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER105W	NP_984965.1
Neurospora crassa	NCU03129	XP_964988.2
Kluyveromyces lactis	KLLA0D02772g	XP_453191.1
Schizosaccharomyces pombe (fission yeast)	SPAC24C9.09	NP_594034.2
Saccharomyces cerevisiae (baker's yeast)	MST1	NP_012727.1
Magnaporthe oryzae (rice blast fungus)	MGG_15672	XP_003714246.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that structural features of the anticodon-binding domain in threonine--tRNA ligase MST1 permit binding of the enlarged anticodon loop of tRNA1(Thr).
Title: The crystal structure of yeast mitochondrial ThrRS in complex with the canonical threonine tRNA.

the plasticity of the aminoacylation site in MST1 allows binding of Ser-AMP and the appropriate positioning of the hydrolytic water molecule.
Title: The mechanism of pre-transfer editing in yeast mitochondrial threonyl-tRNA synthetase.

MST1 recognizes and aminoacylates two natural tRNAs that contain anticodon loops of different size and sequence
Title: Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment.

The following MST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02863	NM_001179760.1 Latest version!	Saccharomyces cerevisiae S288C threonine--tRNA ligase MST1 (MST1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MST1 ( NM_001179760.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012727.1 Saccharomyces cerevisiae S288C threonine--tRNA ligase MST1 (MST1), partial mRNA.

Price & Availability↑

CloneID	OSi02863
Clone ID Related Accession (Same CDS sequence)	NM_001179760.1
Accession Version	NM_001179760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	threonine--tRNA ligase MST1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAATAC AACTTGTAAG ATGGCATTGT TCTCGCAATG CTCTTTGGAA TCGTGCTTTC 
TACTCTACGA GAAAGGCCAC TAAGAACGCT TCGTCGGCGA CTCCAGCTAC TATGACATCC 
ATGGTATCTC AGAGGCAAGA TCTTTTCATG ACAGATCCAC TATCTCCTGG ATCCATGTTC 
TTTCTTCCAA ATGGTGCTAA GATTTTCAAC AAATTGATAG AGTTTATGAA GTTACAACAA 
AAGTTCAAAT TTGGTTTTAA TGAAGTTGTG ACGCCTTTGA TTTACAAAAA GACTCTTTGG 
GAAAAGTCTG GTCACTGGGA AAATTATGCT GATGACATGT TTAAGGTGGA AACTACGGAT 
GAAGAAAAGG AGGAATACGG TTTAAAACCG ATGAATTGCC CAGGCCATTG TCTGATCTTC 
GGTAAAAAGG ATAGATCGTA TAACGAACTT CCCCTACGCT TCTCTGATTT TTCACCACTA 
CATAGAAATG AAGCTTCTGG TGCACTGTCG GGATTGACTA GACTAAGGAA GTTCCATCAA 
GACGATGGTC ATATCTTTTG CACTCCTTCC CAAGTGAAAT CAGAAATTTT CAACTCTTTA 
AAATTAATCG ACATAGTTTA CAACAAAATT TTCCCCTTTG TTAAAGGAGG TTCTGGAGCA 
GAGAGCAATT ATTTTATAAA CTTTTCTACA AGACCGGATC ACTTCATTGG AGACCTCAAA 
GTTTGGAATC ATGCTGAGCA AGTACTCAAG GAAATCTTGG AAGAATCAGG CAAACCGTGG 
AAACTGAACC CTGGTGATGG TGCGTTCTAT GGTCCAAAGT TGGATATAAT GGTTACGGAC 
CATTTACGCA AAACTCATCA AGTCGCTACT ATTCAGTTGG ACTTTCAGCT ACCTGAGAGG 
TTCGATTTGA AATTCAAGGA TCAAGACAAC TCGTACAAGA GGCCAATTAT GATTCATAGA 
GCTACTTTTG GTTCTATCGA AAGATTTATG GCTTTACTGA TAGATTCTAA TGAGGGCCGT 
TGGCCGTTTT GGTTGAATCC TTACCAAGCT GTAATTATTC CCGTCAATAC GAAAAATGTG 
CAACAGCTAG ACATGTGCAC TGCCTTGCAG AAAAAACTAC GTAATGAACT GGAAGCCGAC 
GACATGGAAC CAGTTCCTTT AAATGATTGG CATTTCAATG TTGATCTCGA CATAAGAAAC 
GAACCCGTAG GGTATAGGAT CAAGTCGGCC ATTTTGAAAA ACTATTCCTA TTTAATTATC 
GTGGGTGATG AGGAAGTACA GTTACAGAAA TACAACATTC GAGAACGTGA TAATAGAAAG 
AGTTTCGAGA AACTTACAAT GTCTCAAATT TGGGAAAAAT TTATTGAGCT GGAAAAAAAC 
TACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012727.1
CDS	1..1389
Translation	MKIQLVRWHCSRNALWNRAFYSTRKATKNASSATPATMTSMVSQRQDLFMTDPLSPGSMFFLPNGAKIFNKLIEFMKLQQKFKFGFNEVVTPLIYKKTLWEKSGHWENYADDMFKVETTDEEKEEYGLKPMNCPGHCLIFGKKDRSYNELPLRFSDFSPLHRNEASGALSGLTRLRKFHQDDGHIFCTPSQVKSEIFNSLKLIDIVYNKIFPFVKGGSGAESNYFINFSTRPDHFIGDLKVWNHAEQVLKEILEESGKPWKLNPGDGAFYGPKLDIMVTDHLRKTHQVATIQLDFQLPERFDLKFKDQDNSYKRPIMIHRATFGSIERFMALLIDSNEGRWPFWLNPYQAVIIPVNTKNVQQLDMCTALQKKLRNELEADDMEPVPLNDWHFNVDLDIRNEPVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRERDNRKSFEKLTMSQIWEKFIELEKNYK

Target ORF information:

RefSeq Version	NM_001179760.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C threonine--tRNA ligase MST1 (MST1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179760.1

ORF Insert Sequence:

ATGAAAATAC AACTTGTAAG ATGGCATTGT TCTCGCAATG CTCTTTGGAA TCGTGCTTTC 
TACTCTACGA GAAAGGCCAC TAAGAACGCT TCGTCGGCGA CTCCAGCTAC TATGACATCC 
ATGGTATCTC AGAGGCAAGA TCTTTTCATG ACAGATCCAC TATCTCCTGG ATCCATGTTC 
TTTCTTCCAA ATGGTGCTAA GATTTTCAAC AAATTGATAG AGTTTATGAA GTTACAACAA 
AAGTTCAAAT TTGGTTTTAA TGAAGTTGTG ACGCCTTTGA TTTACAAAAA GACTCTTTGG 
GAAAAGTCTG GTCACTGGGA AAATTATGCT GATGACATGT TTAAGGTGGA AACTACGGAT 
GAAGAAAAGG AGGAATACGG TTTAAAACCG ATGAATTGCC CAGGCCATTG TCTGATCTTC 
GGTAAAAAGG ATAGATCGTA TAACGAACTT CCCCTACGCT TCTCTGATTT TTCACCACTA 
CATAGAAATG AAGCTTCTGG TGCACTGTCG GGATTGACTA GACTAAGGAA GTTCCATCAA 
GACGATGGTC ATATCTTTTG CACTCCTTCC CAAGTGAAAT CAGAAATTTT CAACTCTTTA 
AAATTAATCG ACATAGTTTA CAACAAAATT TTCCCCTTTG TTAAAGGAGG TTCTGGAGCA 
GAGAGCAATT ATTTTATAAA CTTTTCTACA AGACCGGATC ACTTCATTGG AGACCTCAAA 
GTTTGGAATC ATGCTGAGCA AGTACTCAAG GAAATCTTGG AAGAATCAGG CAAACCGTGG 
AAACTGAACC CTGGTGATGG TGCGTTCTAT GGTCCAAAGT TGGATATAAT GGTTACGGAC 
CATTTACGCA AAACTCATCA AGTCGCTACT ATTCAGTTGG ACTTTCAGCT ACCTGAGAGG 
TTCGATTTGA AATTCAAGGA TCAAGACAAC TCGTACAAGA GGCCAATTAT GATTCATAGA 
GCTACTTTTG GTTCTATCGA AAGATTTATG GCTTTACTGA TAGATTCTAA TGAGGGCCGT 
TGGCCGTTTT GGTTGAATCC TTACCAAGCT GTAATTATTC CCGTCAATAC GAAAAATGTG 
CAACAGCTAG ACATGTGCAC TGCCTTGCAG AAAAAACTAC GTAATGAACT GGAAGCCGAC 
GACATGGAAC CAGTTCCTTT AAATGATTGG CATTTCAATG TTGATCTCGA CATAAGAAAC 
GAACCCGTAG GGTATAGGAT CAAGTCGGCC ATTTTGAAAA ACTATTCCTA TTTAATTATC 
GTGGGTGATG AGGAAGTACA GTTACAGAAA TACAACATTC GAGAACGTGA TAATAGAAAG 
AGTTTCGAGA AACTTACAAT GTCTCAAATT TGGGAAAAAT TTATTGAGCT GGAAAAAAAC 
TACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報