EAP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	EAP1
Entrez Gene ID	853631
Full Name	Eap1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179769.1	NP_012718.1	Eap1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the interaction of Vts1p with Eap1p at target mRNAs stimulates decapping and transcript decay
Title: The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay.

SESA network comprising Smy2, Eap1, Scp160 and Asc1 , specifically inhibits initiation of translation of POM34 mRNA that encodes an integral membrane protein of the nuclear pore complex
Title: The SESA network links duplication of the yeast centrosome with the protein translation machinery.

upon TOR-inactivation, the putative TOR-regulated eIF4E-associated protein Eap1p likely functions downstream of Gcn2p to attenuate GCN4 translation via a mechanism independent of eIF4E-binding
Title: The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation.

The following EAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02856	NM_001179769.1 Latest version!	Saccharomyces cerevisiae S288C Eap1p (EAP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EAP1 ( NM_001179769.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012718.1 Saccharomyces cerevisiae S288C Eap1p (EAP1), partial mRNA.

Price & Availability↑

CloneID	OSi02856
Clone ID Related Accession (Same CDS sequence)	NM_001179769.1
Accession Version	NM_001179769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Eap1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAACTCA ACGACCCTTC AATTATATCA AGCAGCCAGT TTTCAGGTGA ATTGTCTGAC 
AGCGATACTG CAGCCGCTAC TCACAAATCG CAACAAGCCA TCTCAAATTT ATTCCAAAAA 
TTAGCCAAAA AGGGCAGAGA AGAGAAGCCA ATCGGTAGCG TAGAATCTTC AACTGATAGC 
TCTAACATTT CTGTTGCTAC GAGCGGTAAC AATAAAGAAA GCAATAAAAA AAAAAATAAG 
AAAACGGCCA TGCTAAATTT CAGCAGTTTG ACGGACCCTA TAACGAACTA CAAACCAATG 
GATCTGCAAT ACAAGACATA TGCATACTCC ATGAATGAAC TTTATCATTT GAAACCATCT 
TTGGCAAGTG CTTCGTACGA AGAAGACCCA CTTATTTCGG AACTTGTGAG GAGTTTACCC 
AAAAGAAAGT TTTGGCGATT GCGCATGGGA CCTCCAGATC AAAAGCATGC TAATAACCAT 
CATTTTAATG GGAACAATGG TGGAGGCAGT TGGAAGGCTG GTTACAAGAA TGGGAAAAAC 
GATGAGAGAA GAATGAGTAG AACCAAAAAC ATGCAGGGAG GCAAACGAAG ATCTCAACAA 
GATGATGAAG AAAAAAAGAT TGACCAAGAA ATGCTGGAGA TGGACAAGAA TCTTCAATTA 
GGAGGTGACG TCGGCCATTC AATTGCAGAT TTTGAAGATT GGAAGGCAAA GATGAAAGAA 
TTAGAATTGA AAAAACTTTC AAAATCTAAA GGAATCAGTA ACTCTACCGC AATTGCTCCC 
AGGGAAAGCG CCTCTCATGA AACACCCACC GATTTAAGAC CGGTCATTCC AAGGGGCCCT 
AGTTCAATAA CAGATTTTTT GAATTTAAAG AGGCAAGATA AGAAGGAAGA ATCTTCACAA 
CAAACACCTG GTATTCCTGT TGGACAGCCA AGCCTATCCA AAACCAGTAT AGAACAAGTT 
AACGAATTAG AAACTAATTC AGATTTGGGT AAAAGTTCAT CATCTCGGTT TTCATCATTT 
TTCAATAAAT CTGCCACTTC GTTGCCTTCA TTAGATAATA ATAATCAAGT GCCATCGTCA 
AACGTGTCTG TAGTAAACAA CGATGGCAAC AGTACACCTC ATCAAAGTGG TTCAAGACTG 
ATGTCGTTTT TCAAAGAATC TAGATCGAGC ACTCCAAATG CAGAATCGCA GTTGTTATCA 
GCCTCAGACA AAGATAATGG AAAGATGCAG ACTCTTCCGC AATTCCAGCA GCAACCGCAA 
CAAATGCAAC CAATGGCATT TACCCAGCAT CCTCCTAATA ACAACGCATT TTTCAATGGG 
CTATTGAATA AAGGTAAAAG TGAAACTAGT ACTCCTCCTC CTCCACCCCC AGGACTAATT 
GCTCATCAGG GTCCACAATT TCCTGTGATG GGTGTACCAC CAAATTTTCC ACAACGCATG 
ATGCCACCGC CACCAGGTCT CGTTCAATTC CAGAAAGATT CTAAAGATGT AAATAAGAAG 
GAAGACAGGC AATTAAGGCA AAATAAAAAT CCAAACGGAA CAAGAAATAG CAAGGGAAAA 
CAAGAGGAAA CAGCAACGCC AGATCTGCCT CAACAGCAAT ACATGCCACC ACCCCCACCT 
CCAGGGTTTT TTCCAATGCA TCCTAACTTT CCTAACGGCC CAATGCCACC ATTACCACAG 
GGGTTCCCAA TCCCACCAAA TGGTATGTTA CCGGTAACAG GACAACAGCC ACAGCCACCT 
TATCCAAATA TGATGCTACA AGGAAATTTC CCCCCTAATT TCCAGCAAGG TTTTGGTAGT 
AACTCCCCTA TGCCAATTCC ATCTATTATT AATGCCAATG GCAAAAATGT TACAAACCAG 
TTACCACCAG GATTAAACTC TAAAAAGAAT ATAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012718.1
CDS	1..1899
Translation	MELNDPSIISSSQFSGELSDSDTAAATHKSQQAISNLFQKLAKKGREEKPIGSVESSTDSSNISVATSGNNKESNKKKNKKTAMLNFSSLTDPITNYKPMDLQYKTYAYSMNELYHLKPSLASASYEEDPLISELVRSLPKRKFWRLRMGPPDQKHANNHHFNGNNGGGSWKAGYKNGKNDERRMSRTKNMQGGKRRSQQDDEEKKIDQEMLEMDKNLQLGGDVGHSIADFEDWKAKMKELELKKLSKSKGISNSTAIAPRESASHETPTDLRPVIPRGPSSITDFLNLKRQDKKEESSQQTPGIPVGQPSLSKTSIEQVNELETNSDLGKSSSSRFSSFFNKSATSLPSLDNNNQVPSSNVSVVNNDGNSTPHQSGSRLMSFFKESRSSTPNAESQLLSASDKDNGKMQTLPQFQQQPQQMQPMAFTQHPPNNNAFFNGLLNKGKSETSTPPPPPPGLIAHQGPQFPVMGVPPNFPQRMMPPPPGLVQFQKDSKDVNKKEDRQLRQNKNPNGTRNSKGKQEETATPDLPQQQYMPPPPPPGFFPMHPNFPNGPMPPLPQGFPIPPNGMLPVTGQQPQPPYPNMMLQGNFPPNFQQGFGSNSPMPIPSIINANGKNVTNQLPPGLNSKKNIK

Target ORF information:

RefSeq Version	NM_001179769.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Eap1p (EAP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179769.1

ORF Insert Sequence:

ATGGAACTCA ACGACCCTTC AATTATATCA AGCAGCCAGT TTTCAGGTGA ATTGTCTGAC 
AGCGATACTG CAGCCGCTAC TCACAAATCG CAACAAGCCA TCTCAAATTT ATTCCAAAAA 
TTAGCCAAAA AGGGCAGAGA AGAGAAGCCA ATCGGTAGCG TAGAATCTTC AACTGATAGC 
TCTAACATTT CTGTTGCTAC GAGCGGTAAC AATAAAGAAA GCAATAAAAA AAAAAATAAG 
AAAACGGCCA TGCTAAATTT CAGCAGTTTG ACGGACCCTA TAACGAACTA CAAACCAATG 
GATCTGCAAT ACAAGACATA TGCATACTCC ATGAATGAAC TTTATCATTT GAAACCATCT 
TTGGCAAGTG CTTCGTACGA AGAAGACCCA CTTATTTCGG AACTTGTGAG GAGTTTACCC 
AAAAGAAAGT TTTGGCGATT GCGCATGGGA CCTCCAGATC AAAAGCATGC TAATAACCAT 
CATTTTAATG GGAACAATGG TGGAGGCAGT TGGAAGGCTG GTTACAAGAA TGGGAAAAAC 
GATGAGAGAA GAATGAGTAG AACCAAAAAC ATGCAGGGAG GCAAACGAAG ATCTCAACAA 
GATGATGAAG AAAAAAAGAT TGACCAAGAA ATGCTGGAGA TGGACAAGAA TCTTCAATTA 
GGAGGTGACG TCGGCCATTC AATTGCAGAT TTTGAAGATT GGAAGGCAAA GATGAAAGAA 
TTAGAATTGA AAAAACTTTC AAAATCTAAA GGAATCAGTA ACTCTACCGC AATTGCTCCC 
AGGGAAAGCG CCTCTCATGA AACACCCACC GATTTAAGAC CGGTCATTCC AAGGGGCCCT 
AGTTCAATAA CAGATTTTTT GAATTTAAAG AGGCAAGATA AGAAGGAAGA ATCTTCACAA 
CAAACACCTG GTATTCCTGT TGGACAGCCA AGCCTATCCA AAACCAGTAT AGAACAAGTT 
AACGAATTAG AAACTAATTC AGATTTGGGT AAAAGTTCAT CATCTCGGTT TTCATCATTT 
TTCAATAAAT CTGCCACTTC GTTGCCTTCA TTAGATAATA ATAATCAAGT GCCATCGTCA 
AACGTGTCTG TAGTAAACAA CGATGGCAAC AGTACACCTC ATCAAAGTGG TTCAAGACTG 
ATGTCGTTTT TCAAAGAATC TAGATCGAGC ACTCCAAATG CAGAATCGCA GTTGTTATCA 
GCCTCAGACA AAGATAATGG AAAGATGCAG ACTCTTCCGC AATTCCAGCA GCAACCGCAA 
CAAATGCAAC CAATGGCATT TACCCAGCAT CCTCCTAATA ACAACGCATT TTTCAATGGG 
CTATTGAATA AAGGTAAAAG TGAAACTAGT ACTCCTCCTC CTCCACCCCC AGGACTAATT 
GCTCATCAGG GTCCACAATT TCCTGTGATG GGTGTACCAC CAAATTTTCC ACAACGCATG 
ATGCCACCGC CACCAGGTCT CGTTCAATTC CAGAAAGATT CTAAAGATGT AAATAAGAAG 
GAAGACAGGC AATTAAGGCA AAATAAAAAT CCAAACGGAA CAAGAAATAG CAAGGGAAAA 
CAAGAGGAAA CAGCAACGCC AGATCTGCCT CAACAGCAAT ACATGCCACC ACCCCCACCT 
CCAGGGTTTT TTCCAATGCA TCCTAACTTT CCTAACGGCC CAATGCCACC ATTACCACAG 
GGGTTCCCAA TCCCACCAAA TGGTATGTTA CCGGTAACAG GACAACAGCC ACAGCCACCT 
TATCCAAATA TGATGCTACA AGGAAATTTC CCCCCTAATT TCCAGCAAGG TTTTGGTAGT 
AACTCCCCTA TGCCAATTCC ATCTATTATT AATGCCAATG GCAAAAATGT TACAAACCAG 
TTACCACCAG GATTAAACTC TAAAAAGAAT ATAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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EAP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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