RAD2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD2
Entrez Gene ID	853174
Full Name	ssDNA endodeoxyribonuclease RAD2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181387.1	NP_011774.1	ssDNA endodeoxyribonuclease RAD2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RAD2	NP_011774.1
Eremothecium gossypii	AGOS_AFR133C	NP_985680.1
Kluyveromyces lactis	KLLA0A09427g	XP_451412.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rad2 and rad27 overexpression increases genomic instability in yeast. Rad2 and Rad27 causes DNA breaks that are processed by different repair functions.
Title: Differential effect of the overexpression of Rad2/XPG family endonucleases on genome integrity in yeast and human cells.

Study shows that Rad2p interacts with PCNA through its proliferating cell nuclear antigen-binding domain (PCNA-BD) and the PCNA-BD of Rad2p plays a role in UV-induced mutagenesis.
Title: The PCNA binding domain of Rad2p plays a role in mutagenesis by modulating the cell cycle in response to DNA damage.

THe data suggest that in contrast to related enzymes (FEN1 and EXO1), the Rad2 active site may be more accessible, which would create an exit route for DNA substrates without a free 5' end.
Title: Crystal structure of the catalytic core of Rad2: insights into the mechanism of substrate binding.

Structure determination of a Rad2-Tfb1PH complex indicates that Rad2 binds to TFIIH using a similar motif as TFIIEalpha uses to bind TFIIH in the pre-initiation complex.
Title: Structural and functional characterization of interactions involving the Tfb1 subunit of TFIIH and the NER factor Rad2.

These observations suggest that RAD2 has a function in coordinating cell cycle regulation and damaged DNA repair.
Title: Mitotic catastrophe induced by overexpression of budding yeast Rad2p.

The following RAD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02472	NM_001181387.1 Latest version!	Saccharomyces cerevisiae S288C ssDNA endodeoxyribonuclease RAD2 (RAD2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD2 ( NM_001181387.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011774.1 Saccharomyces cerevisiae S288C ssDNA endodeoxyribonuclease RAD2 (RAD2), partial mRNA.

Price & Availability↑

CloneID	OSi02472
Clone ID Related Accession (Same CDS sequence)	NM_001181387.1
Accession Version	NM_001181387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ssDNA endodeoxyribonuclease RAD2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTGTGC ATTCATTTTG GGATATTGCA GGTCCTACGG CAAGACCGGT CAGGCTGGAA 
TCCTTGGAAG ATAAGAGAAT GGCAGTAGAT GCCTCCATTT GGATATATCA GTTTTTGAAA 
GCTGTCCGTG ATCAGGAGGG GAATGCAGTG AAGAATTCTC ATATTACTGG GTTCTTTAGA 
AGAATTTGTA AGCTATTATA CTTTGGCATT AGGCCGGTAT TCGTCTTTGA TGGTGGTGTG 
CCCGTATTGA AAAGGGAAAC AATACGGCAG AGGAAAGAAA GAAGACAGGG AAAACGAGAG 
AGTGCGAAAT CCACCGCTAG GAAGCTGCTG GCTTTACAAT TGCAAAACGG CTCAAACGAT 
AACGTAAAAA ACTCTACACC GAGTAGTGGC AGTAGTGTGC AGATCTTCAA ACCTCAGGAC 
GAGTGGGACT TGCCCGATAT ACCGGGATTC AAATACGATA AGGAAGATGC AAGAGTTAAC 
TCTAATAAAA CTTTTGAAAA ACTGATGAAT TCGATAAACG GTGATGGACT GGAAGACATT 
GATTTAGACA CTATAAATCC AGCATCGGCC GAATTTGAAG AGCTGCCAAA GGCCACACAG 
TATTTGATAT TATCGTCGTT GAGGTTGAAA TCAAGACTGA GGATGGGATA CTCGAAAGAA 
CAATTGGAGA CCATCTTCCC AAATAGTATG GATTTCTCAA GATTTCAAAT AGACATGGTG 
AAAAGGAGGA ATTTTTTTAC TCAAAAGTTA ATCAACACTA CTGGGTTTCA GGATGGTGGA 
GCGTCTAAGC TGAACGAGGA GGTGATCAAT AGGATATCCG GCCAAAAGAG TAAAGAGTAC 
AAGTTGACTA AGACTAATAA CGGGTGGATT CTTGGACTAG GGGCAAACGA TGGCTCTGAT 
GCGCAAAAAG CAATCGTAAT AGACGATAAA GATGCAGGCG CTTTGGTAAA GCAGTTAGAT 
AGTAATGCTG AGGACGGGGA CGTGCTCCGA TGGGATGATT TGGAAGATAA TTCCTTAAAA 
ATAGTGCGAC ATGAGTCTTC TAACGCGACG ACAGCTCCAC AGAAAAGGAG TAACCGGTCA 
GAAGATGAGG GATGTGACTC TGATGAATGT GAGTGGGAAG AAGTTGAACT CAAGCCCAAG 
AACGTCAAGT TTGTGGAAGA TTTTTCTTTG AAAGCTGCAA GGTTGCCTTA CATGGGCCAA 
TCCCTAAACA ACGCTGGCAG TAAATCGTTT TTGGACAAAA GACATGATCA GGCATCGCCC 
TCCAAGACTA CTCCAACAAT GAGAATTAGC AGAATCAGCG TGGAAGACGA CGATGAGGAT 
TACCTGAAAC AAATTGAGGA AATTGAAATG ATGGAGGCAG TGCAACTTTC TAAGATGGAA 
AAGAAACCTG AAGCGGACGA CAAATCAAAA ATCGCTAAGC CGGTAACGTC AAAAGGTACA 
GAGGCTCGTC CACCAATAGT ACAGTATGGA TTACTTGGCG CACAACCGGA TAGTAAGCAA 
CCGTATCATG TCACAAATTT GAATAGCAAA TCAGAGTCGG TCATCAAAAG GACCAGCAAG 
ACAGTTCTTT CTGAGTTTAG GCCACCGTCT CAGCAGGAGG ATAAAGGGGC TATTTTAACA 
GAGGGGGAGC AAAACCTGAA TTTTATTTCT CACAAGATAC CACAGTTTGA TTTTAACAAT 
GAAAATTCCC TGCTTTTTCA AAAGAATACC GAGAGTAATG TGTCCCAAGA AGCCACGAAG 
GAAAAGTCTC CAATTCCTGA AATGCCGAGC TGGTTTTCTT CAACTGCTTC CCAGCAGTTG 
TATAATCCCT ATAACACAAC AAATTTCGTG GAAGATAAAA ACGTAAGAAA TGAACAAGAA 
AGTGGAGCAG AAACTACCAA TAAAGGGAGT AGCTATGAAC TCTTGACAGG CTTAAATGCT 
ACGGAAATAT TAGAAAGAGA AAGCGAGAAA GAGAGCTCCA ATGATGAAAA CAAGGACGAT 
GACTTAGAAG TTTTATCAGA AGAACTATTT GAGGACGTTC CCACTAAATC TCAGATTTCA 
AAAGAAGCAG AGGATAACGA TAGTAGAAAA GTCGAATCTA TAAATAAGGA GCATAGAAAG 
CCTCTTATAT TCGACTATGA CTTCTCCGAA GATGAAGAAG ATAACATAGT AGAAAATATG 
ATAAAGGAAC AAGAAGAGTT TGATACATTT AAAAACACGA CTTTGTCCAC TTCTGCTGAA 
AGAAACGTTG CCGAGAACGC TTTTGTGGAG GATGAATTAT TCGAACAACA GATGAAGGAT 
AAAAGAGATT CGGATGAGGT AACTATGGAT ATGATCAAAG AAGTGCAAGA ATTACTATCG 
AGGTTTGGAA TCCCCTATAT CACTGCGCCT ATGGAAGCTG AAGCACAGTG TGCGGAATTG 
TTACAACTAA ACCTTGTCGA TGGTATAATT ACCGATGACA GTGATGTTTT CCTATTTGGA 
GGTACAAAGA TCTACAAAAA TATGTTCCAC GAAAAGAACT ATGTTGAATT TTATGATGCG 
GAATCTATTT TAAAATTATT GGGCTTGGAT AGAAAGAATA TGATTGAGTT GGCACAGCTT 
TTAGGGAGCG ATTACACGAA TGGATTGAAG GGTATGGGTC CCGTTTCAAG CATTGAAGTG 
ATTGCAGAAT TTGGAAACCT AAAAAATTTT AAAGACTGGT ATAATAATGG GCAGTTTGAT 
AAACGTAAGC AAGAAACGGA AAATAAATTT GAAAAAGACC TGAGAAAAAA ACTGGTAAAT 
AACGAAATTA TCTTAGATGA TGATTTTCCT AGCGTCATGG TTTATGATGC GTATATGAGA 
CCAGAAGTCG ATCACGATAC CACGCCGTTT GTTTGGGGGG TACCAGATCT CGATATGCTT 
CGTTCATTCA TGAAGACTCA ACTAGGTTGG CCACACGAAA AGTCTGATGA AATTCTCATT 
CCCTTAATTA GAGATGTTAA TAAACGCAAA AAGAAGGGGA AGCAAAAAAG GATTAATGAA 
TTTTTTCCAA GGGAGTACAT ATCTGGTGAT AAGAAGCTCA ATACAAGTAA GAGAATTTCA 
ACCGCAACAG GTAAACTAAA GAAAAGAAAG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011774.1
CDS	1..3096
Translation	MGVHSFWDIAGPTARPVRLESLEDKRMAVDASIWIYQFLKAVRDQEGNAVKNSHITGFFRRICKLLYFGIRPVFVFDGGVPVLKRETIRQRKERRQGKRESAKSTARKLLALQLQNGSNDNVKNSTPSSGSSVQIFKPQDEWDLPDIPGFKYDKEDARVNSNKTFEKLMNSINGDGLEDIDLDTINPASAEFEELPKATQYLILSSLRLKSRLRMGYSKEQLETIFPNSMDFSRFQIDMVKRRNFFTQKLINTTGFQDGGASKLNEEVINRISGQKSKEYKLTKTNNGWILGLGANDGSDAQKAIVIDDKDAGALVKQLDSNAEDGDVLRWDDLEDNSLKIVRHESSNATTAPQKRSNRSEDEGCDSDECEWEEVELKPKNVKFVEDFSLKAARLPYMGQSLNNAGSKSFLDKRHDQASPSKTTPTMRISRISVEDDDEDYLKQIEEIEMMEAVQLSKMEKKPEADDKSKIAKPVTSKGTEARPPIVQYGLLGAQPDSKQPYHVTNLNSKSESVIKRTSKTVLSEFRPPSQQEDKGAILTEGEQNLNFISHKIPQFDFNNENSLLFQKNTESNVSQEATKEKSPIPEMPSWFSSTASQQLYNPYNTTNFVEDKNVRNEQESGAETTNKGSSYELLTGLNATEILERESEKESSNDENKDDDLEVLSEELFEDVPTKSQISKEAEDNDSRKVESINKEHRKPLIFDYDFSEDEEDNIVENMIKEQEEFDTFKNTTLSTSAERNVAENAFVEDELFEQQMKDKRDSDEVTMDMIKEVQELLSRFGIPYITAPMEAEAQCAELLQLNLVDGIITDDSDVFLFGGTKIYKNMFHEKNYVEFYDAESILKLLGLDRKNMIELAQLLGSDYTNGLKGMGPVSSIEVIAEFGNLKNFKDWYNNGQFDKRKQETENKFEKDLRKKLVNNEIILDDDFPSVMVYDAYMRPEVDHDTTPFVWGVPDLDMLRSFMKTQLGWPHEKSDEILIPLIRDVNKRKKKGKQKRINEFFPREYISGDKKLNTSKRISTATGKLKKRKM

Target ORF information:

RefSeq Version	NM_001181387.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ssDNA endodeoxyribonuclease RAD2 (RAD2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181387.1

ORF Insert Sequence:

ATGGGTGTGC ATTCATTTTG GGATATTGCA GGTCCTACGG CAAGACCGGT CAGGCTGGAA 
TCCTTGGAAG ATAAGAGAAT GGCAGTAGAT GCCTCCATTT GGATATATCA GTTTTTGAAA 
GCTGTCCGTG ATCAGGAGGG GAATGCAGTG AAGAATTCTC ATATTACTGG GTTCTTTAGA 
AGAATTTGTA AGCTATTATA CTTTGGCATT AGGCCGGTAT TCGTCTTTGA TGGTGGTGTG 
CCCGTATTGA AAAGGGAAAC AATACGGCAG AGGAAAGAAA GAAGACAGGG AAAACGAGAG 
AGTGCGAAAT CCACCGCTAG GAAGCTGCTG GCTTTACAAT TGCAAAACGG CTCAAACGAT 
AACGTAAAAA ACTCTACACC GAGTAGTGGC AGTAGTGTGC AGATCTTCAA ACCTCAGGAC 
GAGTGGGACT TGCCCGATAT ACCGGGATTC AAATACGATA AGGAAGATGC AAGAGTTAAC 
TCTAATAAAA CTTTTGAAAA ACTGATGAAT TCGATAAACG GTGATGGACT GGAAGACATT 
GATTTAGACA CTATAAATCC AGCATCGGCC GAATTTGAAG AGCTGCCAAA GGCCACACAG 
TATTTGATAT TATCGTCGTT GAGGTTGAAA TCAAGACTGA GGATGGGATA CTCGAAAGAA 
CAATTGGAGA CCATCTTCCC AAATAGTATG GATTTCTCAA GATTTCAAAT AGACATGGTG 
AAAAGGAGGA ATTTTTTTAC TCAAAAGTTA ATCAACACTA CTGGGTTTCA GGATGGTGGA 
GCGTCTAAGC TGAACGAGGA GGTGATCAAT AGGATATCCG GCCAAAAGAG TAAAGAGTAC 
AAGTTGACTA AGACTAATAA CGGGTGGATT CTTGGACTAG GGGCAAACGA TGGCTCTGAT 
GCGCAAAAAG CAATCGTAAT AGACGATAAA GATGCAGGCG CTTTGGTAAA GCAGTTAGAT 
AGTAATGCTG AGGACGGGGA CGTGCTCCGA TGGGATGATT TGGAAGATAA TTCCTTAAAA 
ATAGTGCGAC ATGAGTCTTC TAACGCGACG ACAGCTCCAC AGAAAAGGAG TAACCGGTCA 
GAAGATGAGG GATGTGACTC TGATGAATGT GAGTGGGAAG AAGTTGAACT CAAGCCCAAG 
AACGTCAAGT TTGTGGAAGA TTTTTCTTTG AAAGCTGCAA GGTTGCCTTA CATGGGCCAA 
TCCCTAAACA ACGCTGGCAG TAAATCGTTT TTGGACAAAA GACATGATCA GGCATCGCCC 
TCCAAGACTA CTCCAACAAT GAGAATTAGC AGAATCAGCG TGGAAGACGA CGATGAGGAT 
TACCTGAAAC AAATTGAGGA AATTGAAATG ATGGAGGCAG TGCAACTTTC TAAGATGGAA 
AAGAAACCTG AAGCGGACGA CAAATCAAAA ATCGCTAAGC CGGTAACGTC AAAAGGTACA 
GAGGCTCGTC CACCAATAGT ACAGTATGGA TTACTTGGCG CACAACCGGA TAGTAAGCAA 
CCGTATCATG TCACAAATTT GAATAGCAAA TCAGAGTCGG TCATCAAAAG GACCAGCAAG 
ACAGTTCTTT CTGAGTTTAG GCCACCGTCT CAGCAGGAGG ATAAAGGGGC TATTTTAACA 
GAGGGGGAGC AAAACCTGAA TTTTATTTCT CACAAGATAC CACAGTTTGA TTTTAACAAT 
GAAAATTCCC TGCTTTTTCA AAAGAATACC GAGAGTAATG TGTCCCAAGA AGCCACGAAG 
GAAAAGTCTC CAATTCCTGA AATGCCGAGC TGGTTTTCTT CAACTGCTTC CCAGCAGTTG 
TATAATCCCT ATAACACAAC AAATTTCGTG GAAGATAAAA ACGTAAGAAA TGAACAAGAA 
AGTGGAGCAG AAACTACCAA TAAAGGGAGT AGCTATGAAC TCTTGACAGG CTTAAATGCT 
ACGGAAATAT TAGAAAGAGA AAGCGAGAAA GAGAGCTCCA ATGATGAAAA CAAGGACGAT 
GACTTAGAAG TTTTATCAGA AGAACTATTT GAGGACGTTC CCACTAAATC TCAGATTTCA 
AAAGAAGCAG AGGATAACGA TAGTAGAAAA GTCGAATCTA TAAATAAGGA GCATAGAAAG 
CCTCTTATAT TCGACTATGA CTTCTCCGAA GATGAAGAAG ATAACATAGT AGAAAATATG 
ATAAAGGAAC AAGAAGAGTT TGATACATTT AAAAACACGA CTTTGTCCAC TTCTGCTGAA 
AGAAACGTTG CCGAGAACGC TTTTGTGGAG GATGAATTAT TCGAACAACA GATGAAGGAT 
AAAAGAGATT CGGATGAGGT AACTATGGAT ATGATCAAAG AAGTGCAAGA ATTACTATCG 
AGGTTTGGAA TCCCCTATAT CACTGCGCCT ATGGAAGCTG AAGCACAGTG TGCGGAATTG 
TTACAACTAA ACCTTGTCGA TGGTATAATT ACCGATGACA GTGATGTTTT CCTATTTGGA 
GGTACAAAGA TCTACAAAAA TATGTTCCAC GAAAAGAACT ATGTTGAATT TTATGATGCG 
GAATCTATTT TAAAATTATT GGGCTTGGAT AGAAAGAATA TGATTGAGTT GGCACAGCTT 
TTAGGGAGCG ATTACACGAA TGGATTGAAG GGTATGGGTC CCGTTTCAAG CATTGAAGTG 
ATTGCAGAAT TTGGAAACCT AAAAAATTTT AAAGACTGGT ATAATAATGG GCAGTTTGAT 
AAACGTAAGC AAGAAACGGA AAATAAATTT GAAAAAGACC TGAGAAAAAA ACTGGTAAAT 
AACGAAATTA TCTTAGATGA TGATTTTCCT AGCGTCATGG TTTATGATGC GTATATGAGA 
CCAGAAGTCG ATCACGATAC CACGCCGTTT GTTTGGGGGG TACCAGATCT CGATATGCTT 
CGTTCATTCA TGAAGACTCA ACTAGGTTGG CCACACGAAA AGTCTGATGA AATTCTCATT 
CCCTTAATTA GAGATGTTAA TAAACGCAAA AAGAAGGGGA AGCAAAAAAG GATTAATGAA 
TTTTTTCCAA GGGAGTACAT ATCTGGTGAT AAGAAGCTCA ATACAAGTAA GAGAATTTCA 
ACCGCAACAG GTAAACTAAA GAAAAGAAAG ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RAD2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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