SNF3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SNF3
Entrez Gene ID	851333
Full Name	glucose sensor
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180254.1	NP_010087.1	glucose sensor

Pathways from BioSystems

Homologies

Neurospora crassa	NCU08180	XP_959573.1
Eremothecium gossypii	AGOS_ADR091W	NP_984187.2
Saccharomyces cerevisiae (baker's yeast)	RGT2	NP_010143.1
Saccharomyces cerevisiae (baker's yeast)	SNF3	NP_010087.1
Kluyveromyces lactis	KLLA0F05181g	XP_455315.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rgt2 and Snf3 glucose sensors are primary targets of methylglyoxal, which causes degradation of the Rgt2 and Snf3.
Title: The glucose metabolite methylglyoxal inhibits expression of the glucose transporter genes by inactivating the cell surface glucose sensors Rgt2 and Snf3 in yeast.

Mth1 and Std1, which are downstream effectors of the Snf3/Rgt2 pathway, were required for viability through mitochondrial function but not fermentative metabolism
Title: Disruption of Snf3/Rgt2 glucose sensors decreases lifespan and caloric restriction effectiveness through Mth1/Std1 by adjusting mitochondrial efficiency in yeast.

Snf3 apparent affinity for glucose was lowest when the intracellular glucose concentration was high. The results conform to an alternating-access model for transporter-like sensors
Title: Conditions with high intracellular glucose inhibit sensing through glucose sensor Snf3 in Saccharomyces cerevisiae.

This study reveals that Snf3, in addition to glucose, also senses fructose and mannose, as well as the glucose analogues 2-deoxyglucose, 3-O-methylglucoside and 6-deoxyglucose.
Title: Amino acid residues involved in ligand preference of the Snf3 transporter-like sensor in Saccharomyces cerevisiae.

Suppression of the snf3 mutant phenotype by deletion of IRA2 was confirmed.
Title: Loss of IRA2 suppresses the growth defect on low glucose caused by the snf3 mutation in Saccharomyces cerevisiae.

The following SNF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00895	NM_001180254.1 Latest version!	Saccharomyces cerevisiae S288C glucose sensor (SNF3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNF3 ( NM_001180254.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010087.1 Saccharomyces cerevisiae S288C glucose sensor (SNF3), partial mRNA.

Price & Availability↑

CloneID	OSi00895
Clone ID Related Accession (Same CDS sequence)	NM_001180254.1
Accession Version	NM_001180254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	glucose sensor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATCCTA ATAGTAACAG TTCTAGCGAA ACATTACGCC AAGAGAAACA GGGTTTCCTA 
GACAAAGCTC TTCAGAGGGT GAAGGGCATA GCACTGCGAC GAAACAATAG TAACAAAGAT 
CATACAACAG ATGATACGAC AGGTAGCATA CGAACCCCTA CGAGCTTGCA GCGGCAAAAT 
TCTGACAGGC AATCTAATAT GACATCCGTG TTTACGGATG ACATTTCTAC CATAGACGAC 
AACTCAATTT TATTTTCAGA GCCTCCTCAG AAACAATCTA TGATGATGTC TATATGCGTA 
GGTGTTTTTG TTGCAGTTGG CGGATTTTTA TTTGGTTATG ATACAGGTCT GATCAACAGT 
ATTACATCTA TGAACTATGT GAAGTCACAC GTAGCACCTA ATCACGATTC ATTTACCGCC 
CAACAAATGT CCATTTTGGT GTCATTTTTG TCATTGGGAA CTTTTTTTGG GGCTTTAACT 
GCACCATTTA TATCTGATTC GTATGGCAGG AAGCCTACTA TCATTTTCAG TACAATTTTC 
ATCTTCTCTA TCGGAAATTC TTTACAGGTA GGTGCTGGAG GAATCACATT ATTGATTGTG 
GGAAGGGTCA TTTCAGGTAT CGGTATAGGC GCAATTTCAG CGGTTGTTCC ATTATACCAA 
GCAGAAGCTA CACATAAATC ATTAAGAGGT GCTATTATTT CTACTTACCA ATGGGCCATT 
ACCTGGGGCT TGCTCGTGTC AAGTGCAGTG TCGCAAGGGA CACACGCAAG AAACGACGCA 
TCTTCGTATC GGATTCCCAT AGGGTTGCAA TATGTCTGGT CGTCATTTCT CGCTATCGGG 
ATGTTCTTTC TCCCTGAGAG TCCACGCTAT TACGTTTTGA AAGACAAGCT AGATGAAGCA 
GCTAAATCTT TATCGTTTTT AAGAGGTGTA CCAGTCCATG ATTCTGGGTT ACTGGAAGAA 
CTAGTTGAAA TAAAGGCAAC ATATGATTAC GAGGCATCTT TTGGTTCTTC GAACTTCATT 
GATTGTTTTA TTTCAAGTAA AAGTAGACCA AAGCAAACTC TAAGGATGTT TACGGGAATT 
GCCCTTCAAG CATTTCAACA ATTTTCAGGT ATCAACTTTA TATTTTACTA CGGTGTCAAT 
TTCTTCAATA AGACAGGAGT CAGTAATAGT TATCTGGTTT CATTTATAAC CTATGCTGTT 
AATGTTGTCT TTAATGTTCC TGGTTTGTTT TTTGTGGAAT TTTTTGGTAG ACGTAAGGTG 
CTGGTTGTTG GGGGTGTTAT CATGACTATA GCCAACTTTA TTGTGGCCAT TGTTGGGTGT 
TCCTTAAAGA CTGTAGCGGC CGCAAAAGTT ATGATAGCAT TTATATGTCT ATTCATAGCT 
GCCTTTTCTG CTACATGGGG TGGTGTTGTT TGGGTTATTT CAGCAGAACT GTACCCATTG 
GGTGTGAGAT CTAAATGTAC GGCTATATGC GCTGCTGCTA ACTGGCTTGT AAACTTTATT 
TGTGCTTTAA TTACCCCTTA TATTGTAGAT ACTGGGTCGC ATACATCATC ATTAGGTGCA 
AAAATATTCT TCATTTGGGG CTCCTTAAAT GCGATGGGGG TGATAGTTGT TTACTTGACC 
GTTTATGAAA CGAAGGGTTT GACATTAGAA GAGATTGATG AATTATATAT TAAGTCATCC 
ACTGGTGTCG TGTCACCAAA ATTTAATAAA GATATTAGGG AACGCGCACT TAAATTCCAA 
TACGATCCTT TGCAAAGATT AGAAGACGGA AAGAACACTT TTGTTGCTAA AAGAAATAAT 
TTTGACGATG AAACACCAAG AAATGATTTT CGAAATACGA TATCGGGCGA AATAGATCAT 
AGTCCCAATC AAAAAGAAGT TCATTCTATC CCAGAACGTG TTGATATTCC TACTAGTACA 
GAAATTCTTG AAAGCCCGAA CAAAAGTAGT GGTATGACAG TCCCTGTGTC ACCTTCTCTG 
CAAGACGTTC CAATCCCGCA AACAACAGAG CCTGCTGAAA TTCGAACCAA ATATGTGGAC 
CTAGGAAATG GGCTTGGTCT TAATACGTAT AATAGAGGGC CTCCCTCACT CTCAAGCGAC 
TCAAGCGAAG ATTACACAGA AGATGAAATA GGCGGGCCCT CATCTCAAGG CGACCAAAGT 
AATAGAAGTA CTATGAATGA TATTAATGAT TATATGGCAC GTCTCATTCA CAGTACTTCT 
ACTGCAAGTA ACACGACAGA TAAGTTCTCC GGTAACCAAA GTACCCTTCG TTACCACACG 
GCTTCCTCAC ATTCGGATAC AACTGAAGAG GACAGCAATT TGATGGACCT GGGAAACGGG 
CTTGCCTTGA ATGCTTATAA CAGAGGTCCA CCTTCAATTT TAATGAATTC CAGTGATGAA 
GAGGCAAATG GTGGTGAGAC GTCTGATAAT TTGAACACAG CTCAAGACTT GGCTGGTATG 
AAGGAACGAA TGGCGCAGTT TGCGCAGAGC TATATTGACA AGAGAGGCGG TCTGGAACCT 
GAAACTCAAT CTAATATTTT GAGCACTTCT CTCTCCGTGA TGGCTGACAC TAATGAACAT 
AATAATGAAA TCCTCCACTC AAGCGAAGAA AACGCCACTA ATCAACCTGT AAATGAAAAT 
AATGATTTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010087.1
CDS	1..2655
Translation	MDPNSNSSSETLRQEKQGFLDKALQRVKGIALRRNNSNKDHTTDDTTGSIRTPTSLQRQNSDRQSNMTSVFTDDISTIDDNSILFSEPPQKQSMMMSICVGVFVAVGGFLFGYDTGLINSITSMNYVKSHVAPNHDSFTAQQMSILVSFLSLGTFFGALTAPFISDSYGRKPTIIFSTIFIFSIGNSLQVGAGGITLLIVGRVISGIGIGAISAVVPLYQAEATHKSLRGAIISTYQWAITWGLLVSSAVSQGTHARNDASSYRIPIGLQYVWSSFLAIGMFFLPESPRYYVLKDKLDEAAKSLSFLRGVPVHDSGLLEELVEIKATYDYEASFGSSNFIDCFISSKSRPKQTLRMFTGIALQAFQQFSGINFIFYYGVNFFNKTGVSNSYLVSFITYAVNVVFNVPGLFFVEFFGRRKVLVVGGVIMTIANFIVAIVGCSLKTVAAAKVMIAFICLFIAAFSATWGGVVWVISAELYPLGVRSKCTAICAAANWLVNFICALITPYIVDTGSHTSSLGAKIFFIWGSLNAMGVIVVYLTVYETKGLTLEEIDELYIKSSTGVVSPKFNKDIRERALKFQYDPLQRLEDGKNTFVAKRNNFDDETPRNDFRNTISGEIDHSPNQKEVHSIPERVDIPTSTEILESPNKSSGMTVPVSPSLQDVPIPQTTEPAEIRTKYVDLGNGLGLNTYNRGPPSLSSDSSEDYTEDEIGGPSSQGDQSNRSTMNDINDYMARLIHSTSTASNTTDKFSGNQSTLRYHTASSHSDTTEEDSNLMDLGNGLALNAYNRGPPSILMNSSDEEANGGETSDNLNTAQDLAGMKERMAQFAQSYIDKRGGLEPETQSNILSTSLSVMADTNEHNNEILHSSEENATNQPVNENNDLK

Target ORF information:

RefSeq Version	NM_001180254.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C glucose sensor (SNF3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180254.1

ORF Insert Sequence:

ATGGATCCTA ATAGTAACAG TTCTAGCGAA ACATTACGCC AAGAGAAACA GGGTTTCCTA 
GACAAAGCTC TTCAGAGGGT GAAGGGCATA GCACTGCGAC GAAACAATAG TAACAAAGAT 
CATACAACAG ATGATACGAC AGGTAGCATA CGAACCCCTA CGAGCTTGCA GCGGCAAAAT 
TCTGACAGGC AATCTAATAT GACATCCGTG TTTACGGATG ACATTTCTAC CATAGACGAC 
AACTCAATTT TATTTTCAGA GCCTCCTCAG AAACAATCTA TGATGATGTC TATATGCGTA 
GGTGTTTTTG TTGCAGTTGG CGGATTTTTA TTTGGTTATG ATACAGGTCT GATCAACAGT 
ATTACATCTA TGAACTATGT GAAGTCACAC GTAGCACCTA ATCACGATTC ATTTACCGCC 
CAACAAATGT CCATTTTGGT GTCATTTTTG TCATTGGGAA CTTTTTTTGG GGCTTTAACT 
GCACCATTTA TATCTGATTC GTATGGCAGG AAGCCTACTA TCATTTTCAG TACAATTTTC 
ATCTTCTCTA TCGGAAATTC TTTACAGGTA GGTGCTGGAG GAATCACATT ATTGATTGTG 
GGAAGGGTCA TTTCAGGTAT CGGTATAGGC GCAATTTCAG CGGTTGTTCC ATTATACCAA 
GCAGAAGCTA CACATAAATC ATTAAGAGGT GCTATTATTT CTACTTACCA ATGGGCCATT 
ACCTGGGGCT TGCTCGTGTC AAGTGCAGTG TCGCAAGGGA CACACGCAAG AAACGACGCA 
TCTTCGTATC GGATTCCCAT AGGGTTGCAA TATGTCTGGT CGTCATTTCT CGCTATCGGG 
ATGTTCTTTC TCCCTGAGAG TCCACGCTAT TACGTTTTGA AAGACAAGCT AGATGAAGCA 
GCTAAATCTT TATCGTTTTT AAGAGGTGTA CCAGTCCATG ATTCTGGGTT ACTGGAAGAA 
CTAGTTGAAA TAAAGGCAAC ATATGATTAC GAGGCATCTT TTGGTTCTTC GAACTTCATT 
GATTGTTTTA TTTCAAGTAA AAGTAGACCA AAGCAAACTC TAAGGATGTT TACGGGAATT 
GCCCTTCAAG CATTTCAACA ATTTTCAGGT ATCAACTTTA TATTTTACTA CGGTGTCAAT 
TTCTTCAATA AGACAGGAGT CAGTAATAGT TATCTGGTTT CATTTATAAC CTATGCTGTT 
AATGTTGTCT TTAATGTTCC TGGTTTGTTT TTTGTGGAAT TTTTTGGTAG ACGTAAGGTG 
CTGGTTGTTG GGGGTGTTAT CATGACTATA GCCAACTTTA TTGTGGCCAT TGTTGGGTGT 
TCCTTAAAGA CTGTAGCGGC CGCAAAAGTT ATGATAGCAT TTATATGTCT ATTCATAGCT 
GCCTTTTCTG CTACATGGGG TGGTGTTGTT TGGGTTATTT CAGCAGAACT GTACCCATTG 
GGTGTGAGAT CTAAATGTAC GGCTATATGC GCTGCTGCTA ACTGGCTTGT AAACTTTATT 
TGTGCTTTAA TTACCCCTTA TATTGTAGAT ACTGGGTCGC ATACATCATC ATTAGGTGCA 
AAAATATTCT TCATTTGGGG CTCCTTAAAT GCGATGGGGG TGATAGTTGT TTACTTGACC 
GTTTATGAAA CGAAGGGTTT GACATTAGAA GAGATTGATG AATTATATAT TAAGTCATCC 
ACTGGTGTCG TGTCACCAAA ATTTAATAAA GATATTAGGG AACGCGCACT TAAATTCCAA 
TACGATCCTT TGCAAAGATT AGAAGACGGA AAGAACACTT TTGTTGCTAA AAGAAATAAT 
TTTGACGATG AAACACCAAG AAATGATTTT CGAAATACGA TATCGGGCGA AATAGATCAT 
AGTCCCAATC AAAAAGAAGT TCATTCTATC CCAGAACGTG TTGATATTCC TACTAGTACA 
GAAATTCTTG AAAGCCCGAA CAAAAGTAGT GGTATGACAG TCCCTGTGTC ACCTTCTCTG 
CAAGACGTTC CAATCCCGCA AACAACAGAG CCTGCTGAAA TTCGAACCAA ATATGTGGAC 
CTAGGAAATG GGCTTGGTCT TAATACGTAT AATAGAGGGC CTCCCTCACT CTCAAGCGAC 
TCAAGCGAAG ATTACACAGA AGATGAAATA GGCGGGCCCT CATCTCAAGG CGACCAAAGT 
AATAGAAGTA CTATGAATGA TATTAATGAT TATATGGCAC GTCTCATTCA CAGTACTTCT 
ACTGCAAGTA ACACGACAGA TAAGTTCTCC GGTAACCAAA GTACCCTTCG TTACCACACG 
GCTTCCTCAC ATTCGGATAC AACTGAAGAG GACAGCAATT TGATGGACCT GGGAAACGGG 
CTTGCCTTGA ATGCTTATAA CAGAGGTCCA CCTTCAATTT TAATGAATTC CAGTGATGAA 
GAGGCAAATG GTGGTGAGAC GTCTGATAAT TTGAACACAG CTCAAGACTT GGCTGGTATG 
AAGGAACGAA TGGCGCAGTT TGCGCAGAGC TATATTGACA AGAGAGGCGG TCTGGAACCT 
GAAACTCAAT CTAATATTTT GAGCACTTCT CTCTCCGTGA TGGCTGACAC TAATGAACAT 
AATAATGAAA TCCTCCACTC AAGCGAAGAA AACGCCACTA ATCAACCTGT AAATGAAAAT 
AATGATTTGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SNF3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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