YRF1-5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YRF1-5
Entrez Gene ID	851189
Full Name	Y' element ATP-dependent helicase protein 1 copy 5
Synonyms	YRF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182355.3	NP_013572.3	Y' element ATP-dependent helicase protein 1 copy 5

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YEL077C	NP_010835.1
Saccharomyces cerevisiae (baker's yeast)	YRF1-7	NP_015040.1
Saccharomyces cerevisiae (baker's yeast)	YPR204W	NP_015530.1
Saccharomyces cerevisiae (baker's yeast)	YHR219W	NP_012091.3
Saccharomyces cerevisiae (baker's yeast)	YJL225C	NP_012311.1
Saccharomyces cerevisiae (baker's yeast)	YRF1-5	NP_013572.3
Saccharomyces cerevisiae (baker's yeast)	YRF1-1	NP_010834.1
Saccharomyces cerevisiae (baker's yeast)	YFL066C	NP_116589.1
Saccharomyces cerevisiae (baker's yeast)	YIL177C	NP_012092.1
Saccharomyces cerevisiae (baker's yeast)	YRF1-6	NP_014060.1
Saccharomyces cerevisiae (baker's yeast)	YBL112C	NP_009438.1
Saccharomyces cerevisiae (baker's yeast)	YRF1-2	NP_011117.3
Saccharomyces cerevisiae (baker's yeast)	YRF1-3	NP_011812.3
Saccharomyces cerevisiae (baker's yeast)	YLL067C	NP_013033.1
Saccharomyces cerevisiae (baker's yeast)	YLL066C	NP_013034.1
Saccharomyces cerevisiae (baker's yeast)	YBL113C	NP_009437.1
Saccharomyces cerevisiae (baker's yeast)	YRF1-8	NP_061495.4
Saccharomyces cerevisiae (baker's yeast)	YML133C	NP_013573.1
Saccharomyces cerevisiae (baker's yeast)	YRF1-4	NP_013571.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YRF1-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YRF1-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00776	NM_001182355.3 Latest version!	Saccharomyces cerevisiae S288C Y' element ATP-dependent helicase protein 1 copy 5 (YRF1-5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YRF1-5 ( NM_001182355.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013572.3 Saccharomyces cerevisiae S288C Y' element ATP-dependent helicase protein 1 copy 5 (YRF1-5), partial mRNA.

Price & Availability↑

CloneID	OSi00776
Clone ID Related Accession (Same CDS sequence)	NM_001182355.3
Accession Version	NM_001182355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Y' element ATP-dependent helicase protein 1 copy 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182355.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAGTTT CCGATAGGCG TAAGTTTGAA AAAGCAAACT TTGACGAGTT TGAGTCGGCT 
CTAAATAACA AAAACGACTT GGTACATTGT CCCTCAATAA CTTTATTTGA ATCGATCCCC 
ACGGAAGTGC GGTCATTCTA CGAAGACGAA AAGTCTGGCC TAATCAAAGT GGTAAAATTC 
AGAACTGGTG CAATGGATAG GAAAAGGTCT TTTGAAAAAA TTGTCATTTC CGTCATGGTC 
GGGAAAAATG TACAAAAGTT CCTGACATTT GTTGAAGACG AACCAGATTT CCAGGGCGGA 
CCAATCCCTT CAAAGTATCT TATTCCCAAG AAAATCAACT TGATGGTCTA CACGTTGTTT 
CAAGTGCATA CTTTGAAATT CAATAGAAAG GATTACGATA CCCTTTCTCT TTTTTACCTC 
AACAGAGGAT ACTATAATGA GTTGAGTTTC CGTGTCCTGG AACGTTGTCA CGAAATAGCG 
AGTGCCAGGC CGAACGACAG CTCTACGATG CGTACTTTCA CTGACTTTGT TTCTGGCGCA 
CCTATTGTAA GGAGTCTTCA GAAAAGCACC ATAAGGAAAT ATGGGTACAA TTTGGCACCC 
TACATGTTCT TGTTACTACA CGTAGATGAG CTATCGATTT TTTCTGCATA CCAAGCAAGT 
TTACCTGGCG AAAAGAAAGT CGACACAGAG CGGCTGAAGC GTGATCTATG CCCACGTAAA 
CCCATTGAGA TAAAGTACTT TTCACAGATA TGTAACGATA TGATGAACAA AAAAGACCGA 
TTGGGTGATA TTTTGCATAT TATCTTGCGA GCATGTGCGC TCAATTTCGG GGCGGGTCCC 
CGTGGTGGCG CTGGTGACGA AGAGGATCGA TCTATTACGA ATGAAGAACC CATTATTCCC 
TCTGTGGACG AGCATGGCTT GAAAGTATGT AAGTTGCGTA GTCCTAACAC TCCACGAAGA 
CTCAGAAAAA CACTAGATGC CGTGAAAGCT TTATTGGTGT CGTCTTGTGC TTGTACTGCA 
AGGGATTTAG ATATATTTGA TGACACCAAC GGCGTTGCAA TGTGGAAATG GATCAAAATT 
CTGTACCACG AAGTAGCGCA GGAAACCACG CTGAAGGACT CTTATAGAAT AACTTTGGTA 
CCTTCTTCTG ATGGTATATC AGTATGTGGA AAACTTTTTA ATCGCGAGTA TGTCCGCGGC 
TTTTACTTTG CATGCAAGGC TCAGTTCGAT AACCTTTGGG GAGAGTTGAA CAACTGCTTT 
TATATGCCTA CAGTGGTTGA TATTGCCAGC CTCATTTTGC GTAATCGAGA AGTTTTGTTC 
AGAGAGCCAA AGCGAGGAAT TGACGAGTAT CTGGAAAACG ATTCTTTTCT TCAAATGATA 
CCTGTTAAAT ATCGTGAAAT TGTGCTGCCC AAGTTGAGAA GAGATACTAA CAAAATGACC 
GCGGCTCTTA AAAATAAAGT CACTGTTGCA ATTGACGAGC TTACGGTGCC ACTTATGTGG 
ATGGTCCATT TTGCCGTAGG ATACCCTTAC CGTTATCCAG AGCTTCAGCT ACTCGCTTTT 
GCCGGTCCTC AGCGCAACGT ATACGTCGAT GATACAACAA GACGCATCCA ACTGTACACT 
GATTACAACA AGAACGGTTC ATCGGAGCCT CGACTTAAGA CGCTTGACGG ACTCACTTCA 
GATTACGTGT TTTATTTTGT CACTGTGCTA AGGCAAATGC AAATATGTGC GCTTGGTAAC 
AGTTATGACG CTTTTAATCA TGATCCTTGG ATGGATGTGG TGGGATTTGA GGATCCAGAT 
CAAGTAACAA ATCGAGACAT TTCGAGGATA GTTTTGTATT CCTACATGTT TCTGAATACC 
GCGAAGGGCT GTCTGGTTGA ATACGCAACT TTTCGGCAGT ACATGAGGGA ACTTCCGAAG 
AATGCACCTC AGAAGCTGAA TTTTCGGGAG ATGCGTCAGG GGTTGATTGC CCTAGGACGG 
CACTGCGTAG GTAGCAGATT TGAAACAGAT TTGTACGAGT CGGCGACGAG TGAACTCATG 
GCCAATCATT CCGTTCAAAC AGGGCGAAAT ATTTACGGTG TGGATTCCTT TTCGTTAACT 
AGTGTCAGTG GGACGACCGC CACTTTATTG CAGGAACGAG CTTCCGAGCG CTGGATTCAA 
TGGTTAGGCC TTGAAAGCGA CTACCATTGT TCATTCTCTA GTACTCGGAA TGCGGAAGAC 
GTAGTGGCAG GTGAGGCGGC GAGTTCAGAT CATGATCAAA AAATTTCAAG AGTAACGCGA 
AAAAGGCCCC GAGAGCCCAA GAGTACAAAC GATATCCTCG TCGCAGGCCA GAAACTCTTT 
GGCAGCTCCT TTGAATTCAG GGACTTGCAT CAGTTGCGCT TATGTCATGA AATATACATG 
GCAGACACAC CCTCTGTGGC AGTACAGGCC CCACCGGGCT ATGGTAAGAC GGAGTTATTT 
CATCTCCCCT TGATAGCACT GGCGTCTAAG GGCGACGTGA AATATGTGTC GTTTCTGTTT 
GTACCGTACA CAGTGTTGCT TGCTAATTGC ATGATCAGGT TGAGCCGATG CGGTTGCTTG 
AATGTGGCCC CTGTAAGAAA CTTTATTGAA GAAGGTTGCG ATGGCGTTAC TGATTTATAC 
GTGGGGATCT ACGATGATCT TGCTAGCACT AATTTCACAG ACAGGATAGC TGCGTGGGAG 
AATATTGTTG AGTGCACCTT TAGGACCAAC AACGTAAAAT TGGGTTACCT CATTGTAGAT 
GAGTTTCACA ACTTTGAAAC GGAGGTCTAC CGGCAGTCGC AATTTGGGGG CATAACTAAC 
CTTGATTTTG ACGCTTTTGA GAAAGCAATC TTTTTGAGCG GCACAGCACC TGAGGCTGTA 
GCTGATGCTG CGTTGCAGCG TATTGGGCTT ACGGGACTGG CCAAGAAGTC GATGGACATC 
AACGAGCTCA AACGGTCGGA AGATCTCAGC AGAGGTCTAT CCAGCTATCC AACACGGATG 
TTTAATCTAA TCAAGGAGAA ATCCGAGGTG CCTTTAGGGC ATGTTCATAA AATTTGGAAG 
AAAGTGGAAT CACAGCCCGA AGAAGCACTG AAGCTTCTTT TAGCCCTCTT TGAAATTGAA 
CCAGAGTCGA AGGCCATTGT AGTTGCAAGC ACAACCAACG AAGTGGAAGA ATTGGCCTGC 
TCTTGGAGAA AGTATTTTAG GGTGGTATGG ATACACGGGA AGCTGGGTGC TGCAGAAAAG 
GTGTCTCGCA CAAAGGAGTT TGTCACTGAC GGTAGCATGC GAGTTCTCAT CGGAACGAAA 
TTAGTGACTG AAGGAATTGA CATTAAGCAA TTGATGATGG TGATCATGCT TGATAATAGA 
CTTAATATTA TTGAGCTCAT TCAAGGCGTA GGGAGACTAA GAGATGGGGG CCTCTGTTAT 
CTATTATCTA GAAAAAACAG TTGGGCGGCA AGGAATCGTA AGGGTGAATT ACCACCGATT 
AAGGAAGGCT GTATAACCGA ACAGGTACGC GAGTTCTATG GACTTGAATC AAAGAAAGGA 
AAAAAGGGCC AGCATGTTGG ATGCTGTGGC TCCAGGACAG ACCTGTCTGC TGACACAGTG 
GAACTGATAG AAAGAATGGA CAGATTGGCT GAAAAACAGG CGACAGCTTC CATGTCGATC 
ATTGCGTTAC CGTCTAGCTT CCAGGAGAGC AATAGCAGTG ACAGGTGCAG AAAGTATTGC 
AGCAGTGATG AGGACAGCGA CACGTGCATT CATGGTAGTG CTAATGCCAG TACCAATGCG 
ACTACCAACT CCAGCACTAA TGCTACTACC ACTGCCAGCA CCAACGTCAG GACTAGTGCT 
ACTACCACTG CCAGCATCAA CGTCAGGACT AGTGCGATTA CCACTGAAAG TACCAACTCC 
AGCACTAATG CTACTACCAC TGCCAGCACC AACGTCAGGA CTAGTGCTAC TACCACTGCC 
AGCATCAACG TCAGGACTAG TGCGACTACC ACTGAAAGTA CCAACTCCAA CACTAGTGCT 
ACTACCACCG AAAGTACCGA CTCCAACACT AGTGCTACTA CCACCGAAAG TACCGACTCC 
AACACTAGTG CTACTACCAC TGCTAGCACC AACTCCAGCA CTAATGCCAC TACCACTGCT 
AGCACCAACT CCAGCACTAA TGCCACTACC ACTGAAAGTA CCAACGCTAG TGCCAAGGAG 
GACGCCAATA AAGATGGCAA TGCTGAGGAT AATAGATTCC ATCCAGTCAC CGACATTAAC 
AAAGAGTCGT ATAAGCGGAA AGGGAGTCAA ATGGTTTTGC TAGAGAGAAA GAAACTGAAA 
GCACAATTTC CCAATACTTC CGAGAATATG AATGTCTTAC AGTTTCTTGG ATTTCGGTCT 
GACGAAATTA AACATCTTTT CCTCTATGGT ATTGACGTAT ACTTCTGCCC AGAGGGAGTA 
TTCACACAAT ACGGATTATG CAAGGGCTGT CAAAAGATGT TCGAGCTCTG TGTCTGTTGG 
GCTGGCCAGA AAGTATCGTA TCGGAGGATG GCTTGGGAAG CACTAGCTGT GGAGAGAATG 
CTGCGAAATG ACGAGGAATA CAAAGAATAC TTGGAAGACA TCGAGCCATA TCATGGGGAC 
CCTGTAGGAT ATTTGAAATA TTTTAGCGTA AAAAGGGGAG AGATCTACTC TCAGATACAG 
AGAAATTATG CTTGGTACCT GGCCATTACT AGAAGAAGAG AAACAATTAG TGTATTGGAT 
TCGACAAGAG GCAAGCAAGG GAGCCAAGTT TTCCGCATGT CTGGAAGGCA GATCAAAGAG 
TTGTATTATA AAGTATGGAG CAACTTGCGT GAATCGAAGA CAGAGGTGCT GCAGTACTTT 
TTGAACTGGG ACGAGAAAAA GTGCCGGGAA GAATGGGAGG CAAAAGACGA TACGGTCTTT 
GTGGAAGCGC TCGAGAAAGT TGGAGTTTTT CAGCGTTTGC GTTCCATGAC GAGCGCTGGA 
CTGCAGGGTC CGCAGTACGT CAAGCTGCAG TTTAGCAGGC ATCATCGACA GTTGAGGAGC 
AGATATGAAT TAAGTCTAGG AATGCACTTG CGAGATCAGC TTGCGCTGGG AGTTACCCCA 
TCTAAAGTGC CGCATTGGAC GGCATTCCTG TCGATGCTGA TAGGGCTGTT CTACAATAAA 
ACATTTCGGC AGAAACTGGA ATATCTTTTG GAGCAGATTT CGGAGGTGTG GTTGTTACCA 
CATTGGCTTG ATTTGGCAAA CGTTGAAGTT CTCGCTGCAG ATAACACGAG GGTACCGCTG 
TACATGCTGA TGGTAGCGGT TCACAAAGAG CTGGATAGCG ATGATGTTCC AGACGGTAGA 
TTTGATATAA TATTACTATG TAGAGATTCG AGCAGAGAAG TTGGAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013572.3
CDS	1..5391
Translation	MKVSDRRKFEKANFDEFESALNNKNDLVHCPSITLFESIPTEVRSFYEDEKSGLIKVVKFRTGAMDRKRSFEKIVISVMVGKNVQKFLTFVEDEPDFQGGPIPSKYLIPKKINLMVYTLFQVHTLKFNRKDYDTLSLFYLNRGYYNELSFRVLERCHEIASARPNDSSTMRTFTDFVSGAPIVRSLQKSTIRKYGYNLAPYMFLLLHVDELSIFSAYQASLPGEKKVDTERLKRDLCPRKPIEIKYFSQICNDMMNKKDRLGDILHIILRACALNFGAGPRGGAGDEEDRSITNEEPIIPSVDEHGLKVCKLRSPNTPRRLRKTLDAVKALLVSSCACTARDLDIFDDTNGVAMWKWIKILYHEVAQETTLKDSYRITLVPSSDGISVCGKLFNREYVRGFYFACKAQFDNLWGELNNCFYMPTVVDIASLILRNREVLFREPKRGIDEYLENDSFLQMIPVKYREIVLPKLRRDTNKMTAALKNKVTVAIDELTVPLMWMVHFAVGYPYRYPELQLLAFAGPQRNVYVDDTTRRIQLYTDYNKNGSSEPRLKTLDGLTSDYVFYFVTVLRQMQICALGNSYDAFNHDPWMDVVGFEDPDQVTNRDISRIVLYSYMFLNTAKGCLVEYATFRQYMRELPKNAPQKLNFREMRQGLIALGRHCVGSRFETDLYESATSELMANHSVQTGRNIYGVDSFSLTSVSGTTATLLQERASERWIQWLGLESDYHCSFSSTRNAEDVVAGEAASSDHDQKISRVTRKRPREPKSTNDILVAGQKLFGSSFEFRDLHQLRLCHEIYMADTPSVAVQAPPGYGKTELFHLPLIALASKGDVKYVSFLFVPYTVLLANCMIRLSRCGCLNVAPVRNFIEEGCDGVTDLYVGIYDDLASTNFTDRIAAWENIVECTFRTNNVKLGYLIVDEFHNFETEVYRQSQFGGITNLDFDAFEKAIFLSGTAPEAVADAALQRIGLTGLAKKSMDINELKRSEDLSRGLSSYPTRMFNLIKEKSEVPLGHVHKIWKKVESQPEEALKLLLALFEIEPESKAIVVASTTNEVEELACSWRKYFRVVWIHGKLGAAEKVSRTKEFVTDGSMRVLIGTKLVTEGIDIKQLMMVIMLDNRLNIIELIQGVGRLRDGGLCYLLSRKNSWAARNRKGELPPIKEGCITEQVREFYGLESKKGKKGQHVGCCGSRTDLSADTVELIERMDRLAEKQATASMSIIALPSSFQESNSSDRCRKYCSSDEDSDTCIHGSANASTNATTNSSTNATTTASTNVRTSATTTASINVRTSAITTESTNSSTNATTTASTNVRTSATTTASINVRTSATTTESTNSNTSATTTESTDSNTSATTTESTDSNTSATTTASTNSSTNATTTASTNSSTNATTTESTNASAKEDANKDGNAEDNRFHPVTDINKESYKRKGSQMVLLERKKLKAQFPNTSENMNVLQFLGFRSDEIKHLFLYGIDVYFCPEGVFTQYGLCKGCQKMFELCVCWAGQKVSYRRMAWEALAVERMLRNDEEYKEYLEDIEPYHGDPVGYLKYFSVKRGEIYSQIQRNYAWYLAITRRRETISVLDSTRGKQGSQVFRMSGRQIKELYYKVWSNLRESKTEVLQYFLNWDEKKCREEWEAKDDTVFVEALEKVGVFQRLRSMTSAGLQGPQYVKLQFSRHHRQLRSRYELSLGMHLRDQLALGVTPSKVPHWTAFLSMLIGLFYNKTFRQKLEYLLEQISEVWLLPHWLDLANVEVLAADNTRVPLYMLMVAVHKELDSDDVPDGRFDIILLCRDSSREVGE

Target ORF information:

RefSeq Version	NM_001182355.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Y' element ATP-dependent helicase protein 1 copy 5 (YRF1-5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182355.3

ORF Insert Sequence:

ATGAAAGTTT CCGATAGGCG TAAGTTTGAA AAAGCAAACT TTGACGAGTT TGAGTCGGCT 
CTAAATAACA AAAACGACTT GGTACATTGT CCCTCAATAA CTTTATTTGA ATCGATCCCC 
ACGGAAGTGC GGTCATTCTA CGAAGACGAA AAGTCTGGCC TAATCAAAGT GGTAAAATTC 
AGAACTGGTG CAATGGATAG GAAAAGGTCT TTTGAAAAAA TTGTCATTTC CGTCATGGTC 
GGGAAAAATG TACAAAAGTT CCTGACATTT GTTGAAGACG AACCAGATTT CCAGGGCGGA 
CCAATCCCTT CAAAGTATCT TATTCCCAAG AAAATCAACT TGATGGTCTA CACGTTGTTT 
CAAGTGCATA CTTTGAAATT CAATAGAAAG GATTACGATA CCCTTTCTCT TTTTTACCTC 
AACAGAGGAT ACTATAATGA GTTGAGTTTC CGTGTCCTGG AACGTTGTCA CGAAATAGCG 
AGTGCCAGGC CGAACGACAG CTCTACGATG CGTACTTTCA CTGACTTTGT TTCTGGCGCA 
CCTATTGTAA GGAGTCTTCA GAAAAGCACC ATAAGGAAAT ATGGGTACAA TTTGGCACCC 
TACATGTTCT TGTTACTACA CGTAGATGAG CTATCGATTT TTTCTGCATA CCAAGCAAGT 
TTACCTGGCG AAAAGAAAGT CGACACAGAG CGGCTGAAGC GTGATCTATG CCCACGTAAA 
CCCATTGAGA TAAAGTACTT TTCACAGATA TGTAACGATA TGATGAACAA AAAAGACCGA 
TTGGGTGATA TTTTGCATAT TATCTTGCGA GCATGTGCGC TCAATTTCGG GGCGGGTCCC 
CGTGGTGGCG CTGGTGACGA AGAGGATCGA TCTATTACGA ATGAAGAACC CATTATTCCC 
TCTGTGGACG AGCATGGCTT GAAAGTATGT AAGTTGCGTA GTCCTAACAC TCCACGAAGA 
CTCAGAAAAA CACTAGATGC CGTGAAAGCT TTATTGGTGT CGTCTTGTGC TTGTACTGCA 
AGGGATTTAG ATATATTTGA TGACACCAAC GGCGTTGCAA TGTGGAAATG GATCAAAATT 
CTGTACCACG AAGTAGCGCA GGAAACCACG CTGAAGGACT CTTATAGAAT AACTTTGGTA 
CCTTCTTCTG ATGGTATATC AGTATGTGGA AAACTTTTTA ATCGCGAGTA TGTCCGCGGC 
TTTTACTTTG CATGCAAGGC TCAGTTCGAT AACCTTTGGG GAGAGTTGAA CAACTGCTTT 
TATATGCCTA CAGTGGTTGA TATTGCCAGC CTCATTTTGC GTAATCGAGA AGTTTTGTTC 
AGAGAGCCAA AGCGAGGAAT TGACGAGTAT CTGGAAAACG ATTCTTTTCT TCAAATGATA 
CCTGTTAAAT ATCGTGAAAT TGTGCTGCCC AAGTTGAGAA GAGATACTAA CAAAATGACC 
GCGGCTCTTA AAAATAAAGT CACTGTTGCA ATTGACGAGC TTACGGTGCC ACTTATGTGG 
ATGGTCCATT TTGCCGTAGG ATACCCTTAC CGTTATCCAG AGCTTCAGCT ACTCGCTTTT 
GCCGGTCCTC AGCGCAACGT ATACGTCGAT GATACAACAA GACGCATCCA ACTGTACACT 
GATTACAACA AGAACGGTTC ATCGGAGCCT CGACTTAAGA CGCTTGACGG ACTCACTTCA 
GATTACGTGT TTTATTTTGT CACTGTGCTA AGGCAAATGC AAATATGTGC GCTTGGTAAC 
AGTTATGACG CTTTTAATCA TGATCCTTGG ATGGATGTGG TGGGATTTGA GGATCCAGAT 
CAAGTAACAA ATCGAGACAT TTCGAGGATA GTTTTGTATT CCTACATGTT TCTGAATACC 
GCGAAGGGCT GTCTGGTTGA ATACGCAACT TTTCGGCAGT ACATGAGGGA ACTTCCGAAG 
AATGCACCTC AGAAGCTGAA TTTTCGGGAG ATGCGTCAGG GGTTGATTGC CCTAGGACGG 
CACTGCGTAG GTAGCAGATT TGAAACAGAT TTGTACGAGT CGGCGACGAG TGAACTCATG 
GCCAATCATT CCGTTCAAAC AGGGCGAAAT ATTTACGGTG TGGATTCCTT TTCGTTAACT 
AGTGTCAGTG GGACGACCGC CACTTTATTG CAGGAACGAG CTTCCGAGCG CTGGATTCAA 
TGGTTAGGCC TTGAAAGCGA CTACCATTGT TCATTCTCTA GTACTCGGAA TGCGGAAGAC 
GTAGTGGCAG GTGAGGCGGC GAGTTCAGAT CATGATCAAA AAATTTCAAG AGTAACGCGA 
AAAAGGCCCC GAGAGCCCAA GAGTACAAAC GATATCCTCG TCGCAGGCCA GAAACTCTTT 
GGCAGCTCCT TTGAATTCAG GGACTTGCAT CAGTTGCGCT TATGTCATGA AATATACATG 
GCAGACACAC CCTCTGTGGC AGTACAGGCC CCACCGGGCT ATGGTAAGAC GGAGTTATTT 
CATCTCCCCT TGATAGCACT GGCGTCTAAG GGCGACGTGA AATATGTGTC GTTTCTGTTT 
GTACCGTACA CAGTGTTGCT TGCTAATTGC ATGATCAGGT TGAGCCGATG CGGTTGCTTG 
AATGTGGCCC CTGTAAGAAA CTTTATTGAA GAAGGTTGCG ATGGCGTTAC TGATTTATAC 
GTGGGGATCT ACGATGATCT TGCTAGCACT AATTTCACAG ACAGGATAGC TGCGTGGGAG 
AATATTGTTG AGTGCACCTT TAGGACCAAC AACGTAAAAT TGGGTTACCT CATTGTAGAT 
GAGTTTCACA ACTTTGAAAC GGAGGTCTAC CGGCAGTCGC AATTTGGGGG CATAACTAAC 
CTTGATTTTG ACGCTTTTGA GAAAGCAATC TTTTTGAGCG GCACAGCACC TGAGGCTGTA 
GCTGATGCTG CGTTGCAGCG TATTGGGCTT ACGGGACTGG CCAAGAAGTC GATGGACATC 
AACGAGCTCA AACGGTCGGA AGATCTCAGC AGAGGTCTAT CCAGCTATCC AACACGGATG 
TTTAATCTAA TCAAGGAGAA ATCCGAGGTG CCTTTAGGGC ATGTTCATAA AATTTGGAAG 
AAAGTGGAAT CACAGCCCGA AGAAGCACTG AAGCTTCTTT TAGCCCTCTT TGAAATTGAA 
CCAGAGTCGA AGGCCATTGT AGTTGCAAGC ACAACCAACG AAGTGGAAGA ATTGGCCTGC 
TCTTGGAGAA AGTATTTTAG GGTGGTATGG ATACACGGGA AGCTGGGTGC TGCAGAAAAG 
GTGTCTCGCA CAAAGGAGTT TGTCACTGAC GGTAGCATGC GAGTTCTCAT CGGAACGAAA 
TTAGTGACTG AAGGAATTGA CATTAAGCAA TTGATGATGG TGATCATGCT TGATAATAGA 
CTTAATATTA TTGAGCTCAT TCAAGGCGTA GGGAGACTAA GAGATGGGGG CCTCTGTTAT 
CTATTATCTA GAAAAAACAG TTGGGCGGCA AGGAATCGTA AGGGTGAATT ACCACCGATT 
AAGGAAGGCT GTATAACCGA ACAGGTACGC GAGTTCTATG GACTTGAATC AAAGAAAGGA 
AAAAAGGGCC AGCATGTTGG ATGCTGTGGC TCCAGGACAG ACCTGTCTGC TGACACAGTG 
GAACTGATAG AAAGAATGGA CAGATTGGCT GAAAAACAGG CGACAGCTTC CATGTCGATC 
ATTGCGTTAC CGTCTAGCTT CCAGGAGAGC AATAGCAGTG ACAGGTGCAG AAAGTATTGC 
AGCAGTGATG AGGACAGCGA CACGTGCATT CATGGTAGTG CTAATGCCAG TACCAATGCG 
ACTACCAACT CCAGCACTAA TGCTACTACC ACTGCCAGCA CCAACGTCAG GACTAGTGCT 
ACTACCACTG CCAGCATCAA CGTCAGGACT AGTGCGATTA CCACTGAAAG TACCAACTCC 
AGCACTAATG CTACTACCAC TGCCAGCACC AACGTCAGGA CTAGTGCTAC TACCACTGCC 
AGCATCAACG TCAGGACTAG TGCGACTACC ACTGAAAGTA CCAACTCCAA CACTAGTGCT 
ACTACCACCG AAAGTACCGA CTCCAACACT AGTGCTACTA CCACCGAAAG TACCGACTCC 
AACACTAGTG CTACTACCAC TGCTAGCACC AACTCCAGCA CTAATGCCAC TACCACTGCT 
AGCACCAACT CCAGCACTAA TGCCACTACC ACTGAAAGTA CCAACGCTAG TGCCAAGGAG 
GACGCCAATA AAGATGGCAA TGCTGAGGAT AATAGATTCC ATCCAGTCAC CGACATTAAC 
AAAGAGTCGT ATAAGCGGAA AGGGAGTCAA ATGGTTTTGC TAGAGAGAAA GAAACTGAAA 
GCACAATTTC CCAATACTTC CGAGAATATG AATGTCTTAC AGTTTCTTGG ATTTCGGTCT 
GACGAAATTA AACATCTTTT CCTCTATGGT ATTGACGTAT ACTTCTGCCC AGAGGGAGTA 
TTCACACAAT ACGGATTATG CAAGGGCTGT CAAAAGATGT TCGAGCTCTG TGTCTGTTGG 
GCTGGCCAGA AAGTATCGTA TCGGAGGATG GCTTGGGAAG CACTAGCTGT GGAGAGAATG 
CTGCGAAATG ACGAGGAATA CAAAGAATAC TTGGAAGACA TCGAGCCATA TCATGGGGAC 
CCTGTAGGAT ATTTGAAATA TTTTAGCGTA AAAAGGGGAG AGATCTACTC TCAGATACAG 
AGAAATTATG CTTGGTACCT GGCCATTACT AGAAGAAGAG AAACAATTAG TGTATTGGAT 
TCGACAAGAG GCAAGCAAGG GAGCCAAGTT TTCCGCATGT CTGGAAGGCA GATCAAAGAG 
TTGTATTATA AAGTATGGAG CAACTTGCGT GAATCGAAGA CAGAGGTGCT GCAGTACTTT 
TTGAACTGGG ACGAGAAAAA GTGCCGGGAA GAATGGGAGG CAAAAGACGA TACGGTCTTT 
GTGGAAGCGC TCGAGAAAGT TGGAGTTTTT CAGCGTTTGC GTTCCATGAC GAGCGCTGGA 
CTGCAGGGTC CGCAGTACGT CAAGCTGCAG TTTAGCAGGC ATCATCGACA GTTGAGGAGC 
AGATATGAAT TAAGTCTAGG AATGCACTTG CGAGATCAGC TTGCGCTGGG AGTTACCCCA 
TCTAAAGTGC CGCATTGGAC GGCATTCCTG TCGATGCTGA TAGGGCTGTT CTACAATAAA 
ACATTTCGGC AGAAACTGGA ATATCTTTTG GAGCAGATTT CGGAGGTGTG GTTGTTACCA 
CATTGGCTTG ATTTGGCAAA CGTTGAAGTT CTCGCTGCAG ATAACACGAG GGTACCGCTG 
TACATGCTGA TGGTAGCGGT TCACAAAGAG CTGGATAGCG ATGATGTTCC AGACGGTAGA 
TTTGATATAA TATTACTATG TAGAGATTCG AGCAGAGAAG TTGGAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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YRF1-5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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