VPS33 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS33
Entrez Gene ID	851112
Full Name	tethering complex ATP-binding subunit VPS33
Synonyms	CLS14,MET27,PEP14,SLP1,VAM5,VPL25,VPT33
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182284.3	NP_013500.3	tethering complex ATP-binding subunit VPS33

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B03267g	XP_451679.1
Saccharomyces cerevisiae (baker's yeast)	VPS33	NP_013500.3
Eremothecium gossypii	AGOS_AER064C	NP_984924.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.
Title: A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.

Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes.
Title: The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane.

Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified.
Title: The Habc domain of the SNARE Vam3 interacts with the HOPS tethering complex to facilitate vacuole fusion.

Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes
Title: SM proteins Sly1 and Vps33 co-assemble with Sec17 and SNARE complexes to oppose SNARE disassembly by Sec18.

we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association
Title: Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.

The following VPS33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00711	NM_001182284.3 Latest version!	Saccharomyces cerevisiae S288C tethering complex ATP-binding subunit VPS33 (VPS33), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS33 ( NM_001182284.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013500.3 Saccharomyces cerevisiae S288C tethering complex ATP-binding subunit VPS33 (VPS33), partial mRNA.

Price & Availability↑

CloneID	OSi00711
Clone ID Related Accession (Same CDS sequence)	NM_001182284.3
Accession Version	NM_001182284.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	tethering complex ATP-binding subunit VPS33
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182284.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATAGAT TTTGGAATAC TAAGAAATTT TCATTAACAA ATGCCGATGG ACTATGTGCT 
ACCTTAAATG AGATATCTCA AAATGATGAA GTTCTTGTGG TTCAACCAAG TGTATTGCCA 
GTACTCAATA GTTTGCTAAC TTTCCAAGAT TTGACTCAAT CAACTCCTGT AAGGAAAATT 
ACGTTACTCG ATGATCAGCT AAGTGACGAT TTACCGAGTG CCTTAGGCAG CGTTCCGCAA 
ATGGATCTTA TTTTTCTTAT TGATGTCAGA ACATCTCTCC GACTCCCTCC ACAACTGCTT 
GATGCTGCTC AAAAGCACAA TTTATCATCT TTGCATATAA TATACTGTCG ATGGAAACCG 
TCTTTCCAAA ATACTTTGGA GGATACAGAG CAATGGCAAA AGGATGGTTT CGATTTGAAT 
TCAAAAAAAA CACATTTCCC TAACGTCATT GAATCTCAGT TAAAGGAGCT ATCGAACGAA 
TATACCCTTT ACCCTTGGGA TCTCTTGCCC TTCCCACAGA TTGATGAAAA TGTTCTATTG 
ACTCATTCCC TTTATAACAT GGAAAATGTA AACATGTATT ATCCCAACTT ACGTTCTTTG 
CAGAGTGCCA CAGAGTCAAT ACTGGTTGAT GATATGGTCA ATTCGTTGCA GAGCTTGATT 
TTTGAAACTA ATAGTATCAT AACAAATGTT GTGTCGATAG GTAATCTGTC TAAGAGATGT 
AGCCATCTTT TGAAGAAACG AATCGATGAG CATCAAACAG AGAATGATTT ATTCATCAAG 
GGTACGCTTT ATGGTGAACG AACCAACTGT GGACTAGAAA TGGACTTGAT TATCTTGGAA 
AGGAATACCG ATCCTATAAC GCCATTGTTG ACACAACTTA CGTATGCAGG AATACTAGAT 
GATCTATATG AATTCAATTC TGGCATAAAG ATAAAGGAGA AAGACATGAA CTTCAATTAT 
AAGGAAGATA AAATATGGAA TGATTTGAAA TTTTTAAATT TTGGGTCGAT TGGGCCGCAG 
TTAAATAAAT TGGCAAAGGA ACTACAAACG CAATATGATA CAAGGCATAA AGCCGAGAGC 
GTACATGAAA TCAAAGAATT CGTTGATTCC TTAGGTTCTT TGCAACAAAG GCAAGCTTTT 
TTGAAAAATC ACACAACCTT ATCATCCGAC GTTTTGAAAG TGGTAGAGAC TGAAGAGTAC 
GGATCTTTCA ATAAAATCTT AGAGTTAGAG CTGGAAATTT TGATGGGAAA TACACTTAAT 
AACGACATTG AAGATATTAT ACTCGAGTTG CAGTACCAGT ACGAGGTTGA TCAAAAGAAG 
ATTCTCAGAT TAATCTGTTT ATTGTCTCTT TGTAAAAATT CACTTCGAGA AAAGGATTAT 
GAATATCTAA GAACCTTTAT GATCGACTCT TGGGGCATTG AAAAATGCTT TCAACTTGAA 
TCATTGGCTG AGTTAGGATT TTTCACTAGC AAAACGGGAA AAACTGATTT GCATATTACA 
ACAAGTAAGT CAACAAGATT ACAGAAAGAA TACCGTTATA TTTCACAATG GTTCAATACA 
GTACCCATAG AAGACGAGCA TGCTGCCGAT AAAATCACAA ATGAGAACGA TGACTTCTCG 
GAAGCCACTT TTGCTTACAG TGGTGTAGTG CCCTTGACAA TGAGACTGGT TCAGATGTTA 
TATGATAGGT CTATCTTGTT CCATAATTAT TCCTCGCAGC AGCCTTTTAT ACTGTCAAGA 
GAACCTAGAG TTTCTCAAAC GGAGGATTTA ATTGAACAGT TATATGGAGA CTCACATGCG 
ATCGAAGAGA GTATATGGGT CCCGGGAACC ATTACAAAAA AGATCAATGC AAGCATCAAG 
AGCAATAATA GACGGTCCAT AGACGGATCT AATGGGACAT TTCATGCTGC AGAGGATATT 
GCACTCGTAG TATTCCTCGG AGGTGTAACA ATGGGTGAAA TAGCTATAAT GAAGCATTTG 
CAAAAAATAC TAGGTAAAAA AGGTATCAAT AAAAGGTTTA TCATCATCGC CGATGGCTTG 
ATCAATGGCA CAAGGATCAT GAACTCTATA TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013500.3
CDS	1..2076
Translation	MNRFWNTKKFSLTNADGLCATLNEISQNDEVLVVQPSVLPVLNSLLTFQDLTQSTPVRKITLLDDQLSDDLPSALGSVPQMDLIFLIDVRTSLRLPPQLLDAAQKHNLSSLHIIYCRWKPSFQNTLEDTEQWQKDGFDLNSKKTHFPNVIESQLKELSNEYTLYPWDLLPFPQIDENVLLTHSLYNMENVNMYYPNLRSLQSATESILVDDMVNSLQSLIFETNSIITNVVSIGNLSKRCSHLLKKRIDEHQTENDLFIKGTLYGERTNCGLEMDLIILERNTDPITPLLTQLTYAGILDDLYEFNSGIKIKEKDMNFNYKEDKIWNDLKFLNFGSIGPQLNKLAKELQTQYDTRHKAESVHEIKEFVDSLGSLQQRQAFLKNHTTLSSDVLKVVETEEYGSFNKILELELEILMGNTLNNDIEDIILELQYQYEVDQKKILRLICLLSLCKNSLREKDYEYLRTFMIDSWGIEKCFQLESLAELGFFTSKTGKTDLHITTSKSTRLQKEYRYISQWFNTVPIEDEHAADKITNENDDFSEATFAYSGVVPLTMRLVQMLYDRSILFHNYSSQQPFILSREPRVSQTEDLIEQLYGDSHAIEESIWVPGTITKKINASIKSNNRRSIDGSNGTFHAAEDIALVVFLGGVTMGEIAIMKHLQKILGKKGINKRFIIIADGLINGTRIMNSIS

Target ORF information:

RefSeq Version	NM_001182284.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C tethering complex ATP-binding subunit VPS33 (VPS33), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182284.3

ORF Insert Sequence:

ATGAATAGAT TTTGGAATAC TAAGAAATTT TCATTAACAA ATGCCGATGG ACTATGTGCT 
ACCTTAAATG AGATATCTCA AAATGATGAA GTTCTTGTGG TTCAACCAAG TGTATTGCCA 
GTACTCAATA GTTTGCTAAC TTTCCAAGAT TTGACTCAAT CAACTCCTGT AAGGAAAATT 
ACGTTACTCG ATGATCAGCT AAGTGACGAT TTACCGAGTG CCTTAGGCAG CGTTCCGCAA 
ATGGATCTTA TTTTTCTTAT TGATGTCAGA ACATCTCTCC GACTCCCTCC ACAACTGCTT 
GATGCTGCTC AAAAGCACAA TTTATCATCT TTGCATATAA TATACTGTCG ATGGAAACCG 
TCTTTCCAAA ATACTTTGGA GGATACAGAG CAATGGCAAA AGGATGGTTT CGATTTGAAT 
TCAAAAAAAA CACATTTCCC TAACGTCATT GAATCTCAGT TAAAGGAGCT ATCGAACGAA 
TATACCCTTT ACCCTTGGGA TCTCTTGCCC TTCCCACAGA TTGATGAAAA TGTTCTATTG 
ACTCATTCCC TTTATAACAT GGAAAATGTA AACATGTATT ATCCCAACTT ACGTTCTTTG 
CAGAGTGCCA CAGAGTCAAT ACTGGTTGAT GATATGGTCA ATTCGTTGCA GAGCTTGATT 
TTTGAAACTA ATAGTATCAT AACAAATGTT GTGTCGATAG GTAATCTGTC TAAGAGATGT 
AGCCATCTTT TGAAGAAACG AATCGATGAG CATCAAACAG AGAATGATTT ATTCATCAAG 
GGTACGCTTT ATGGTGAACG AACCAACTGT GGACTAGAAA TGGACTTGAT TATCTTGGAA 
AGGAATACCG ATCCTATAAC GCCATTGTTG ACACAACTTA CGTATGCAGG AATACTAGAT 
GATCTATATG AATTCAATTC TGGCATAAAG ATAAAGGAGA AAGACATGAA CTTCAATTAT 
AAGGAAGATA AAATATGGAA TGATTTGAAA TTTTTAAATT TTGGGTCGAT TGGGCCGCAG 
TTAAATAAAT TGGCAAAGGA ACTACAAACG CAATATGATA CAAGGCATAA AGCCGAGAGC 
GTACATGAAA TCAAAGAATT CGTTGATTCC TTAGGTTCTT TGCAACAAAG GCAAGCTTTT 
TTGAAAAATC ACACAACCTT ATCATCCGAC GTTTTGAAAG TGGTAGAGAC TGAAGAGTAC 
GGATCTTTCA ATAAAATCTT AGAGTTAGAG CTGGAAATTT TGATGGGAAA TACACTTAAT 
AACGACATTG AAGATATTAT ACTCGAGTTG CAGTACCAGT ACGAGGTTGA TCAAAAGAAG 
ATTCTCAGAT TAATCTGTTT ATTGTCTCTT TGTAAAAATT CACTTCGAGA AAAGGATTAT 
GAATATCTAA GAACCTTTAT GATCGACTCT TGGGGCATTG AAAAATGCTT TCAACTTGAA 
TCATTGGCTG AGTTAGGATT TTTCACTAGC AAAACGGGAA AAACTGATTT GCATATTACA 
ACAAGTAAGT CAACAAGATT ACAGAAAGAA TACCGTTATA TTTCACAATG GTTCAATACA 
GTACCCATAG AAGACGAGCA TGCTGCCGAT AAAATCACAA ATGAGAACGA TGACTTCTCG 
GAAGCCACTT TTGCTTACAG TGGTGTAGTG CCCTTGACAA TGAGACTGGT TCAGATGTTA 
TATGATAGGT CTATCTTGTT CCATAATTAT TCCTCGCAGC AGCCTTTTAT ACTGTCAAGA 
GAACCTAGAG TTTCTCAAAC GGAGGATTTA ATTGAACAGT TATATGGAGA CTCACATGCG 
ATCGAAGAGA GTATATGGGT CCCGGGAACC ATTACAAAAA AGATCAATGC AAGCATCAAG 
AGCAATAATA GACGGTCCAT AGACGGATCT AATGGGACAT TTCATGCTGC AGAGGATATT 
GCACTCGTAG TATTCCTCGG AGGTGTAACA ATGGGTGAAA TAGCTATAAT GAAGCATTTG 
CAAAAAATAC TAGGTAAAAA AGGTATCAAT AAAAGGTTTA TCATCATCGC CGATGGCTTG 
ATCAATGGCA CAAGGATCAT GAACTCTATA TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VPS33 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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