SHM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SHM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00410 NM_001181945.1
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Saccharomyces cerevisiae S288C glycine hydroxymethyltransferase SHM2 (SHM2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi00410
    Clone ID Related Accession (Same CDS sequence) NM_001181945.1
    Accession Version NM_001181945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product glycine hydroxymethyltransferase SHM2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTTACA CTCTATCCGA CGCTCATCAT AAGTTGATCA CCTCTCATTT GGTGGACACC 
    GACCCTGAAG TGGACTCCAT TATCAAGGAT GAAATTGAAA GACAAAAGCA CTCCATCGAT
    TTGATTGCTT CTGAAAATTT CACCTCAACC TCCGTTTTCG ATGCCCTTGG AACTCCATTG
    TCCAACAAAT ACTCTGAAGG TTATCCAGGT GCTCGTTACT ACGGTGGTAA TGAACACATT
    GACAGAATGG AAATTCTATG TCAACAAAGA GCTTTGAAAG CTTTCCATGT TACTCCAGAC
    AAATGGGGTG TTAACGTCCA AACTTTATCT GGTTCTCCTG CTAACTTGCA GGTTTATCAA
    GCTATTATGA AGCCTCATGA AAGATTGATG GGTCTATACC TACCAGATGG TGGTCATTTG
    TCTCACGGTT ACGCTACTGA AAACAGAAAA ATTTCTGCTG TTTCCACATA CTTCGAATCT
    TTCCCATACA GAGTTAACCC AGAAACCGGT ATTATCGACT ACGATACTTT AGAAAAGAAC
    GCCATCCTAT ATAGACCAAA GGTTCTTGTT GCTGGTACTT CAGCATACTG TCGTTTAATT
    GACTACAAGA GAATGAGAGA AATCGCCGAC AAATGTGGTG CTTACTTGAT GGTAGACATG
    GCCCACATTT CTGGTTTGAT CGCCGCAGGT GTCATCCCAT CTCCTTTCGA ATACGCTGAT
    ATCGTTACCA CCACCACTCA CAAGTCTTTG AGAGGTCCAC GTGGTGCTAT GATTTTCTTC
    AGAAGAGGTG TGAGATCTAT CAACCCTAAG ACCGGTAAGG AAGTTCTATA CGACTTGGAA
    AACCCAATTA ACTTCTCTGT TTTCCCAGGT CACCAAGGTG GTCCACACAA CCATACCATT
    GCTGCTTTGG CCACTGCTTT GAAGCAAGCT GCCACTCCAG AATTCAAGGA ATACCAAACT
    CAAGTCTTGA AGAATGCTAA GGCTTTGGAA AGTGAATTTA AGAACTTGGG CTACAGATTA
    GTTTCCAACG GTACCGATTC TCACATGGTT CTAGTATCCT TGAGAGAAAA GGGTGTTGAT
    GGTGCTCGTG TTGAATACAT TTGTGAAAAG ATTAACATTG CTTTGAACAA AAACTCTATT
    CCAGGTGACA AATCTGCTTT GGTTCCAGGT GGTGTCCGTA TTGGGGCTCC AGCCATGACC
    ACTAGAGGAA TGGGTGAAGA AGATTTCCAC AGAATTGTTC AATACATTAA CAAGGCTGTA
    GAATTCGCTC AACAAGTTCA ACAAAGCTTG CCAAAGGATG CTTGTAGATT AAAGGACTTC
    AAAGCCAAGG TCGACGAAGG CTCTGATGTT TTGAACACCT GGAAAAAGGA AATTTACGAC
    TGGGCTGGCG AATACCCATT GGCTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_013159.1
    CDS1..1410
    Translation

    Target ORF information:

    RefSeq Version NM_001181945.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C glycine hydroxymethyltransferase SHM2 (SHM2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001181945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTTACA CTCTATCCGA CGCTCATCAT AAGTTGATCA CCTCTCATTT GGTGGACACC 
    GACCCTGAAG TGGACTCCAT TATCAAGGAT GAAATTGAAA GACAAAAGCA CTCCATCGAT
    TTGATTGCTT CTGAAAATTT CACCTCAACC TCCGTTTTCG ATGCCCTTGG AACTCCATTG
    TCCAACAAAT ACTCTGAAGG TTATCCAGGT GCTCGTTACT ACGGTGGTAA TGAACACATT
    GACAGAATGG AAATTCTATG TCAACAAAGA GCTTTGAAAG CTTTCCATGT TACTCCAGAC
    AAATGGGGTG TTAACGTCCA AACTTTATCT GGTTCTCCTG CTAACTTGCA GGTTTATCAA
    GCTATTATGA AGCCTCATGA AAGATTGATG GGTCTATACC TACCAGATGG TGGTCATTTG
    TCTCACGGTT ACGCTACTGA AAACAGAAAA ATTTCTGCTG TTTCCACATA CTTCGAATCT
    TTCCCATACA GAGTTAACCC AGAAACCGGT ATTATCGACT ACGATACTTT AGAAAAGAAC
    GCCATCCTAT ATAGACCAAA GGTTCTTGTT GCTGGTACTT CAGCATACTG TCGTTTAATT
    GACTACAAGA GAATGAGAGA AATCGCCGAC AAATGTGGTG CTTACTTGAT GGTAGACATG
    GCCCACATTT CTGGTTTGAT CGCCGCAGGT GTCATCCCAT CTCCTTTCGA ATACGCTGAT
    ATCGTTACCA CCACCACTCA CAAGTCTTTG AGAGGTCCAC GTGGTGCTAT GATTTTCTTC
    AGAAGAGGTG TGAGATCTAT CAACCCTAAG ACCGGTAAGG AAGTTCTATA CGACTTGGAA
    AACCCAATTA ACTTCTCTGT TTTCCCAGGT CACCAAGGTG GTCCACACAA CCATACCATT
    GCTGCTTTGG CCACTGCTTT GAAGCAAGCT GCCACTCCAG AATTCAAGGA ATACCAAACT
    CAAGTCTTGA AGAATGCTAA GGCTTTGGAA AGTGAATTTA AGAACTTGGG CTACAGATTA
    GTTTCCAACG GTACCGATTC TCACATGGTT CTAGTATCCT TGAGAGAAAA GGGTGTTGAT
    GGTGCTCGTG TTGAATACAT TTGTGAAAAG ATTAACATTG CTTTGAACAA AAACTCTATT
    CCAGGTGACA AATCTGCTTT GGTTCCAGGT GGTGTCCGTA TTGGGGCTCC AGCCATGACC
    ACTAGAGGAA TGGGTGAAGA AGATTTCCAC AGAATTGTTC AATACATTAA CAAGGCTGTA
    GAATTCGCTC AACAAGTTCA ACAAAGCTTG CCAAAGGATG CTTGTAGATT AAAGGACTTC
    AAAGCCAAGG TCGACGAAGG CTCTGATGTT TTGAACACCT GGAAAAAGGA AATTTACGAC
    TGGGCTGGCG AATACCCATT GGCTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
    Nature387(6632 Suppl)87-90(1997 May)
    Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG