Shmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shmt1
Entrez Gene ID	20425
Full Name	serine hydroxymethyltransferase 1 (soluble)
Synonyms	AI324848,AI385541,C81125,Shmt,mshmt,mshmt1,mshmt2
General protein information	Preferred Names serine hydroxymethyltransferase 1 (soluble) Names serine hydroxymethyltransferase, cytosolic glycine hydroxymethyltransferase serine methylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_009171.2	NP_033197.2	serine hydroxymethyltransferase, cytosolic
XM_006532644.4	XP_006532707.1	serine hydroxymethyltransferase, cytosolic isoform X1
XM_006532644.3	XP_006532707.1	serine hydroxymethyltransferase, cytosolic isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SHMT1	XP_005620199.1
Bos taurus (cattle)	SHMT1	NP_001015553.1
Gallus gallus (chicken)	SHMT1	XP_004945260.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001065	XP_003437197.1
Pan troglodytes (chimpanzee)	SHMT1	XP_001157513.1
Homo sapiens (human)	SHMT1	NP_004160.3
Mus musculus (house mouse)	Shmt1	NP_033197.2
Rattus norvegicus (Norway rat)	Shmt1	NP_001041307.1
Kluyveromyces lactis	KLLA0F08921g	XP_455485.1
Arabidopsis thaliana (thale cress)	SHM2	NP_568488.2
	SHMT1	XP_001096314.1
Danio rerio (zebrafish)	shmt1	NP_957340.1
Saccharomyces cerevisiae (baker's yeast)	SHM2	NP_013159.1
Eremothecium gossypii	AGOS_ACR215C	NP_983617.2
Magnaporthe oryzae (rice blast fungus)	MGG_00923	XP_003718036.1
Neurospora crassa	NCU02274	XP_959918.1
Drosophila melanogaster (fruit fly)	CG3011	NP_572278.1
Caenorhabditis elegans (roundworm)	mel-32	NP_741197.1
Schizosaccharomyces pombe (fission yeast)	SPAC24C9.12c	NP_594037.1
Xenopus tropicalis (tropical clawed frog)	shmt1	NP_001015914.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency.
Field MS, Kamynina E, Agunloye OC, Liebenthal RP, Lamarre SG, Brosnan ME, Brosnan JT, Stover PJ
The Journal of biological chemistry289(43)29642-50(2014 Oct)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Disruption of shmt1 impairs hippocampal neurogenesis and mnemonic function in mice.
Abarinov EV, Beaudin AE, Field MS, Perry CA, Allen RH, Stabler SP, Stover PJ
The Journal of nutrition143(7)1028-35(2013 Jul)

Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
Nilsson LM, Forshell TZ, Rimpi S, Kreutzer C, Pretsch W, Bornkamm GW, Nilsson JA
PLoS genetics8(3)e1002573(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Shmt1 and dietary folate deficiency influence metabolic markers of homocysteine remethylation in mice.
Title: Disruption of shmt1 impairs hippocampal neurogenesis and mnemonic function in mice.

SHMT1 and TYMS localization to the nucleus is essential to prevent uracil accumulation in DNA
Title: Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA.

These results provide evidence that disruption of Shmt1 expression causes NTDs by impairing thymidylate biosynthesis and shows that changes in the expression of genes that encode folate-dependent enzymes may be key determinates of NTD risk.
Title: Shmt1 and de novo thymidylate biosynthesis underlie folate-responsive neural tube defects in mice.

Data show that Shmt1 hemizygosity was associated with increased risk for intestinal cancer in Apc(min)(/+) mice through a gene-by-diet interaction.
Title: Shmt1 heterozygosity impairs folate-dependent thymidylate synthesis capacity and modifies risk of Apc(min)-mediated intestinal cancer risk.

It is one of the genetic components regulating PPI in mice and is relevant to schizophrenia susceptibility in humans. (review)
Title: [Analysis of mouse strain-dependent prepulse inhibition points to a role for Shmt1 (SHMT1) in mice and in schizophrenia].

The following Shmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03659	NM_009171.2 Latest version!	Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu03659	XM_006532644.4 Latest version!	Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu03659	XM_006532644.3	Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shmt1 ( NM_009171.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033197.2 Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), mRNA.

Price & Availability↑

CloneID	OMu03659
Clone ID Related Accession (Same CDS sequence)	NM_009171.2 , XM_006532644.4 , XM_006532644.3
Accession Version	NM_009171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	serine hydroxymethyltransferase, cytosolic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF159149.1, BQ942592.1, BQ832130.1 and AI385541.1. On Jun 16, 2005 this sequence version replaced NM_009171.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK010439.1, AK132342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACA GGGATGCCAC CCTGTGGGCT TCTCATGAGA AGATGTTGTC GCAGCCCCTC 
AAGGACAGTG ATGCCGAGGT TTACAGCATC ATTAAAAAGG AGAGCAACCG GCAGAGGGTC 
GGATTAGAGC TGATTGCCTC GGAGAATTTT GCCAGCCGAG CTGTGTTGGA GGCCCTGGGA 
TCTTGCTTAA ATAACAAATA CTCTGAGGGA TATCCAGGCC AAAGGTATTA TGGCGGGACC 
GAGTTCATCG ATGAGCTGGA GATGCTGTGT CAGAAGCGTG CATTACAGGC CTACCATCTG 
GATCCTCAGT GCTGGGGTGT CAATGTCCAG CCTTATTCAG GCTCCCCTGC AAACTTTGCT 
GTGTATACTG CCCTGGTAGA GCCTCATGGG CGAATCATGG GCTTGGACCT GCCAGACGGT 
GGCCATCTGA CCCATGGCTT CATGACAGAC AAGAAGAAAA TCTCTGCCAC ATCTATCTTC 
TTTGAATCCA TGCCTTATAA GGTGTACCCA GAGACTGGGT ACATCAACTA TGACCAGCTG 
GAGGAGAATG CCAGCCTCTT CCACCCGAAG CTGATCATCG CAGGGACCAG CTGCTACTCC 
CGGAACCTGG ACTACGCACG GCTGCGGAAG ATTGCGGATG ATAATGGGGC GTATCTCATG 
GCAGACATGG CACATATCAG TGGGCTCGTG GCAGCTGGGG TGGTGCCCTC CCCCTTTGAA 
CACTGCCATG TAGTGACCAC TACAACCCAC AAGACCCTGA GAGGCTGCCG TGCTGGTATG 
ATATTCTACA GAAAGGGTGT GCGCAGTGTG GATCCCAAGA CTGGCAAAGA GACTTACTAT 
GAACTGGAGT CCCTCATCAA CTCTGCCGTG TTCCCAGGCC TTCAGGGGGG TCCTCACAAC 
CATGCCATTG CTGGTGTTGC TGTGGCCCTG AAGCAAGCCA TGACTACAGA ATTCAAAATC 
TACCAGCTCC AGGTGTTGGC CAACTGCAGG GCTCTGTCTG ATGCACTCAC AGAGCTAGGC 
TACAAAATAG TCACAGGTGG CTCTGATAAT CACTTGATCC TAATGGATCT GCGTTCTAAG 
GGTACTGATG GTGGAAGGGC TGAGAAGGTA CTAGAAGCCT GTTCTATCGC CTGCAACAAG 
AATACCTGCC CAGGTGACAA GAGCGCGTTA CGGCCCAGTG GACTGCGACT GGGGACTCCA 
GCATTGACAT CCCGAGGACT TTTGGAAGAA GACTTCCAAA AAGTAGCACA CTTTATCCAC 
AGGGGGATAG AGTTGACACT GCAGATTCAG AGCCACATGG CCACGAAGGC CACACTGAAA 
GAGTTCAAAG AGAAGCTGGC AGGAGATGAG AAGATCCAGA GTGCTGTGGC AACTCTCCGG 
GAAGAAGTGG AGAACTTTGC TTCCAACTTC TCACTACCTG GCCTTCCTGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033197.2
CDS	382..1818
Translation	MADRDATLWASHEKMLSQPLKDSDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELEMLCQKRALQAYHLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVYPETGYINYDQLEENASLFHPKLIIAGTSCYSRNLDYARLRKIADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVRSVDPKTGKETYYELESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVTGGSDNHLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKVAHFIHRGIELTLQIQSHMATKATLKEFKEKLAGDEKIQSAVATLREEVENFASNFSLPGLPDF

Target ORF information:

RefSeq Version	NM_009171.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009171.2

ORF Insert Sequence:

ATGGCAGACA GGGATGCCAC CCTGTGGGCT TCTCATGAGA AGATGTTGTC GCAGCCCCTC 
AAGGACAGTG ATGCCGAGGT TTACAGCATC ATTAAAAAGG AGAGCAACCG GCAGAGGGTC 
GGATTAGAGC TGATTGCCTC GGAGAATTTT GCCAGCCGAG CTGTGTTGGA GGCCCTGGGA 
TCTTGCTTAA ATAACAAATA CTCTGAGGGA TATCCAGGCC AAAGGTATTA TGGCGGGACC 
GAGTTCATCG ATGAGCTGGA GATGCTGTGT CAGAAGCGTG CATTACAGGC CTACCATCTG 
GATCCTCAGT GCTGGGGTGT CAATGTCCAG CCTTATTCAG GCTCCCCTGC AAACTTTGCT 
GTGTATACTG CCCTGGTAGA GCCTCATGGG CGAATCATGG GCTTGGACCT GCCAGACGGT 
GGCCATCTGA CCCATGGCTT CATGACAGAC AAGAAGAAAA TCTCTGCCAC ATCTATCTTC 
TTTGAATCCA TGCCTTATAA GGTGTACCCA GAGACTGGGT ACATCAACTA TGACCAGCTG 
GAGGAGAATG CCAGCCTCTT CCACCCGAAG CTGATCATCG CAGGGACCAG CTGCTACTCC 
CGGAACCTGG ACTACGCACG GCTGCGGAAG ATTGCGGATG ATAATGGGGC GTATCTCATG 
GCAGACATGG CACATATCAG TGGGCTCGTG GCAGCTGGGG TGGTGCCCTC CCCCTTTGAA 
CACTGCCATG TAGTGACCAC TACAACCCAC AAGACCCTGA GAGGCTGCCG TGCTGGTATG 
ATATTCTACA GAAAGGGTGT GCGCAGTGTG GATCCCAAGA CTGGCAAAGA GACTTACTAT 
GAACTGGAGT CCCTCATCAA CTCTGCCGTG TTCCCAGGCC TTCAGGGGGG TCCTCACAAC 
CATGCCATTG CTGGTGTTGC TGTGGCCCTG AAGCAAGCCA TGACTACAGA ATTCAAAATC 
TACCAGCTCC AGGTGTTGGC CAACTGCAGG GCTCTGTCTG ATGCACTCAC AGAGCTAGGC 
TACAAAATAG TCACAGGTGG CTCTGATAAT CACTTGATCC TAATGGATCT GCGTTCTAAG 
GGTACTGATG GTGGAAGGGC TGAGAAGGTA CTAGAAGCCT GTTCTATCGC CTGCAACAAG 
AATACCTGCC CAGGTGACAA GAGCGCGTTA CGGCCCAGTG GACTGCGACT GGGGACTCCA 
GCATTGACAT CCCGAGGACT TTTGGAAGAA GACTTCCAAA AAGTAGCACA CTTTATCCAC 
AGGGGGATAG AGTTGACACT GCAGATTCAG AGCCACATGG CCACGAAGGC CACACTGAAA 
GAGTTCAAAG AGAAGCTGGC AGGAGATGAG AAGATCCAGA GTGCTGTGGC AACTCTCCGG 
GAAGAAGTGG AGAACTTTGC TTCCAACTTC TCACTACCTG GCCTTCCTGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shmt1 ( XM_006532644.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532707.1 Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03659
Clone ID Related Accession (Same CDS sequence)	NM_009171.2 , XM_006532644.4 , XM_006532644.3
Accession Version	XM_006532644.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	serine hydroxymethyltransferase, cytosolic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532644.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACA GGGATGCCAC CCTGTGGGCT TCTCATGAGA AGATGTTGTC GCAGCCCCTC 
AAGGACAGTG ATGCCGAGGT TTACAGCATC ATTAAAAAGG AGAGCAACCG GCAGAGGGTC 
GGATTAGAGC TGATTGCCTC GGAGAATTTT GCCAGCCGAG CTGTGTTGGA GGCCCTGGGA 
TCTTGCTTAA ATAACAAATA CTCTGAGGGA TATCCAGGCC AAAGGTATTA TGGCGGGACC 
GAGTTCATCG ATGAGCTGGA GATGCTGTGT CAGAAGCGTG CATTACAGGC CTACCATCTG 
GATCCTCAGT GCTGGGGTGT CAATGTCCAG CCTTATTCAG GCTCCCCTGC AAACTTTGCT 
GTGTATACTG CCCTGGTAGA GCCTCATGGG CGAATCATGG GCTTGGACCT GCCAGACGGT 
GGCCATCTGA CCCATGGCTT CATGACAGAC AAGAAGAAAA TCTCTGCCAC ATCTATCTTC 
TTTGAATCCA TGCCTTATAA GGTGTACCCA GAGACTGGGT ACATCAACTA TGACCAGCTG 
GAGGAGAATG CCAGCCTCTT CCACCCGAAG CTGATCATCG CAGGGACCAG CTGCTACTCC 
CGGAACCTGG ACTACGCACG GCTGCGGAAG ATTGCGGATG ATAATGGGGC GTATCTCATG 
GCAGACATGG CACATATCAG TGGGCTCGTG GCAGCTGGGG TGGTGCCCTC CCCCTTTGAA 
CACTGCCATG TAGTGACCAC TACAACCCAC AAGACCCTGA GAGGCTGCCG TGCTGGTATG 
ATATTCTACA GAAAGGGTGT GCGCAGTGTG GATCCCAAGA CTGGCAAAGA GACTTACTAT 
GAACTGGAGT CCCTCATCAA CTCTGCCGTG TTCCCAGGCC TTCAGGGGGG TCCTCACAAC 
CATGCCATTG CTGGTGTTGC TGTGGCCCTG AAGCAAGCCA TGACTACAGA ATTCAAAATC 
TACCAGCTCC AGGTGTTGGC CAACTGCAGG GCTCTGTCTG ATGCACTCAC AGAGCTAGGC 
TACAAAATAG TCACAGGTGG CTCTGATAAT CACTTGATCC TAATGGATCT GCGTTCTAAG 
GGTACTGATG GTGGAAGGGC TGAGAAGGTA CTAGAAGCCT GTTCTATCGC CTGCAACAAG 
AATACCTGCC CAGGTGACAA GAGCGCGTTA CGGCCCAGTG GACTGCGACT GGGGACTCCA 
GCATTGACAT CCCGAGGACT TTTGGAAGAA GACTTCCAAA AAGTAGCACA CTTTATCCAC 
AGGGGGATAG AGTTGACACT GCAGATTCAG AGCCACATGG CCACGAAGGC CACACTGAAA 
GAGTTCAAAG AGAAGCTGGC AGGAGATGAG AAGATCCAGA GTGCTGTGGC AACTCTCCGG 
GAAGAAGTGG AGAACTTTGC TTCCAACTTC TCACTACCTG GCCTTCCTGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532707.1
CDS	179..1615
Translation	MADRDATLWASHEKMLSQPLKDSDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELEMLCQKRALQAYHLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVYPETGYINYDQLEENASLFHPKLIIAGTSCYSRNLDYARLRKIADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVRSVDPKTGKETYYELESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVTGGSDNHLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKVAHFIHRGIELTLQIQSHMATKATLKEFKEKLAGDEKIQSAVATLREEVENFASNFSLPGLPDF

Target ORF information:

RefSeq Version	XM_006532644.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532644.3

ORF Insert Sequence:

ATGGCAGACA GGGATGCCAC CCTGTGGGCT TCTCATGAGA AGATGTTGTC GCAGCCCCTC 
AAGGACAGTG ATGCCGAGGT TTACAGCATC ATTAAAAAGG AGAGCAACCG GCAGAGGGTC 
GGATTAGAGC TGATTGCCTC GGAGAATTTT GCCAGCCGAG CTGTGTTGGA GGCCCTGGGA 
TCTTGCTTAA ATAACAAATA CTCTGAGGGA TATCCAGGCC AAAGGTATTA TGGCGGGACC 
GAGTTCATCG ATGAGCTGGA GATGCTGTGT CAGAAGCGTG CATTACAGGC CTACCATCTG 
GATCCTCAGT GCTGGGGTGT CAATGTCCAG CCTTATTCAG GCTCCCCTGC AAACTTTGCT 
GTGTATACTG CCCTGGTAGA GCCTCATGGG CGAATCATGG GCTTGGACCT GCCAGACGGT 
GGCCATCTGA CCCATGGCTT CATGACAGAC AAGAAGAAAA TCTCTGCCAC ATCTATCTTC 
TTTGAATCCA TGCCTTATAA GGTGTACCCA GAGACTGGGT ACATCAACTA TGACCAGCTG 
GAGGAGAATG CCAGCCTCTT CCACCCGAAG CTGATCATCG CAGGGACCAG CTGCTACTCC 
CGGAACCTGG ACTACGCACG GCTGCGGAAG ATTGCGGATG ATAATGGGGC GTATCTCATG 
GCAGACATGG CACATATCAG TGGGCTCGTG GCAGCTGGGG TGGTGCCCTC CCCCTTTGAA 
CACTGCCATG TAGTGACCAC TACAACCCAC AAGACCCTGA GAGGCTGCCG TGCTGGTATG 
ATATTCTACA GAAAGGGTGT GCGCAGTGTG GATCCCAAGA CTGGCAAAGA GACTTACTAT 
GAACTGGAGT CCCTCATCAA CTCTGCCGTG TTCCCAGGCC TTCAGGGGGG TCCTCACAAC 
CATGCCATTG CTGGTGTTGC TGTGGCCCTG AAGCAAGCCA TGACTACAGA ATTCAAAATC 
TACCAGCTCC AGGTGTTGGC CAACTGCAGG GCTCTGTCTG ATGCACTCAC AGAGCTAGGC 
TACAAAATAG TCACAGGTGG CTCTGATAAT CACTTGATCC TAATGGATCT GCGTTCTAAG 
GGTACTGATG GTGGAAGGGC TGAGAAGGTA CTAGAAGCCT GTTCTATCGC CTGCAACAAG 
AATACCTGCC CAGGTGACAA GAGCGCGTTA CGGCCCAGTG GACTGCGACT GGGGACTCCA 
GCATTGACAT CCCGAGGACT TTTGGAAGAA GACTTCCAAA AAGTAGCACA CTTTATCCAC 
AGGGGGATAG AGTTGACACT GCAGATTCAG AGCCACATGG CCACGAAGGC CACACTGAAA 
GAGTTCAAAG AGAAGCTGGC AGGAGATGAG AAGATCCAGA GTGCTGTGGC AACTCTCCGG 
GAAGAAGTGG AGAACTTTGC TTCCAACTTC TCACTACCTG GCCTTCCTGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shmt1 ( XM_006532644.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532707.1 Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu03659
Clone ID Related Accession (Same CDS sequence)	NM_009171.2 , XM_006532644.4 , XM_006532644.3
Accession Version	XM_006532644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	serine hydroxymethyltransferase, cytosolic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006532644.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACA GGGATGCCAC CCTGTGGGCT TCTCATGAGA AGATGTTGTC GCAGCCCCTC 
AAGGACAGTG ATGCCGAGGT TTACAGCATC ATTAAAAAGG AGAGCAACCG GCAGAGGGTC 
GGATTAGAGC TGATTGCCTC GGAGAATTTT GCCAGCCGAG CTGTGTTGGA GGCCCTGGGA 
TCTTGCTTAA ATAACAAATA CTCTGAGGGA TATCCAGGCC AAAGGTATTA TGGCGGGACC 
GAGTTCATCG ATGAGCTGGA GATGCTGTGT CAGAAGCGTG CATTACAGGC CTACCATCTG 
GATCCTCAGT GCTGGGGTGT CAATGTCCAG CCTTATTCAG GCTCCCCTGC AAACTTTGCT 
GTGTATACTG CCCTGGTAGA GCCTCATGGG CGAATCATGG GCTTGGACCT GCCAGACGGT 
GGCCATCTGA CCCATGGCTT CATGACAGAC AAGAAGAAAA TCTCTGCCAC ATCTATCTTC 
TTTGAATCCA TGCCTTATAA GGTGTACCCA GAGACTGGGT ACATCAACTA TGACCAGCTG 
GAGGAGAATG CCAGCCTCTT CCACCCGAAG CTGATCATCG CAGGGACCAG CTGCTACTCC 
CGGAACCTGG ACTACGCACG GCTGCGGAAG ATTGCGGATG ATAATGGGGC GTATCTCATG 
GCAGACATGG CACATATCAG TGGGCTCGTG GCAGCTGGGG TGGTGCCCTC CCCCTTTGAA 
CACTGCCATG TAGTGACCAC TACAACCCAC AAGACCCTGA GAGGCTGCCG TGCTGGTATG 
ATATTCTACA GAAAGGGTGT GCGCAGTGTG GATCCCAAGA CTGGCAAAGA GACTTACTAT 
GAACTGGAGT CCCTCATCAA CTCTGCCGTG TTCCCAGGCC TTCAGGGGGG TCCTCACAAC 
CATGCCATTG CTGGTGTTGC TGTGGCCCTG AAGCAAGCCA TGACTACAGA ATTCAAAATC 
TACCAGCTCC AGGTGTTGGC CAACTGCAGG GCTCTGTCTG ATGCACTCAC AGAGCTAGGC 
TACAAAATAG TCACAGGTGG CTCTGATAAT CACTTGATCC TAATGGATCT GCGTTCTAAG 
GGTACTGATG GTGGAAGGGC TGAGAAGGTA CTAGAAGCCT GTTCTATCGC CTGCAACAAG 
AATACCTGCC CAGGTGACAA GAGCGCGTTA CGGCCCAGTG GACTGCGACT GGGGACTCCA 
GCATTGACAT CCCGAGGACT TTTGGAAGAA GACTTCCAAA AAGTAGCACA CTTTATCCAC 
AGGGGGATAG AGTTGACACT GCAGATTCAG AGCCACATGG CCACGAAGGC CACACTGAAA 
GAGTTCAAAG AGAAGCTGGC AGGAGATGAG AAGATCCAGA GTGCTGTGGC AACTCTCCGG 
GAAGAAGTGG AGAACTTTGC TTCCAACTTC TCACTACCTG GCCTTCCTGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532707.1
CDS	224..1660
Translation	MADRDATLWASHEKMLSQPLKDSDAEVYSIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELEMLCQKRALQAYHLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVYPETGYINYDQLEENASLFHPKLIIAGTSCYSRNLDYARLRKIADDNGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVRSVDPKTGKETYYELESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTTEFKIYQLQVLANCRALSDALTELGYKIVTGGSDNHLILMDLRSKGTDGGRAEKVLEACSIACNKNTCPGDKSALRPSGLRLGTPALTSRGLLEEDFQKVAHFIHRGIELTLQIQSHMATKATLKEFKEKLAGDEKIQSAVATLREEVENFASNFSLPGLPDF

Target ORF information:

RefSeq Version	XM_006532644.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus serine hydroxymethyltransferase 1 (soluble) (Shmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532644.4

ORF Insert Sequence:

ATGGCAGACA GGGATGCCAC CCTGTGGGCT TCTCATGAGA AGATGTTGTC GCAGCCCCTC 
AAGGACAGTG ATGCCGAGGT TTACAGCATC ATTAAAAAGG AGAGCAACCG GCAGAGGGTC 
GGATTAGAGC TGATTGCCTC GGAGAATTTT GCCAGCCGAG CTGTGTTGGA GGCCCTGGGA 
TCTTGCTTAA ATAACAAATA CTCTGAGGGA TATCCAGGCC AAAGGTATTA TGGCGGGACC 
GAGTTCATCG ATGAGCTGGA GATGCTGTGT CAGAAGCGTG CATTACAGGC CTACCATCTG 
GATCCTCAGT GCTGGGGTGT CAATGTCCAG CCTTATTCAG GCTCCCCTGC AAACTTTGCT 
GTGTATACTG CCCTGGTAGA GCCTCATGGG CGAATCATGG GCTTGGACCT GCCAGACGGT 
GGCCATCTGA CCCATGGCTT CATGACAGAC AAGAAGAAAA TCTCTGCCAC ATCTATCTTC 
TTTGAATCCA TGCCTTATAA GGTGTACCCA GAGACTGGGT ACATCAACTA TGACCAGCTG 
GAGGAGAATG CCAGCCTCTT CCACCCGAAG CTGATCATCG CAGGGACCAG CTGCTACTCC 
CGGAACCTGG ACTACGCACG GCTGCGGAAG ATTGCGGATG ATAATGGGGC GTATCTCATG 
GCAGACATGG CACATATCAG TGGGCTCGTG GCAGCTGGGG TGGTGCCCTC CCCCTTTGAA 
CACTGCCATG TAGTGACCAC TACAACCCAC AAGACCCTGA GAGGCTGCCG TGCTGGTATG 
ATATTCTACA GAAAGGGTGT GCGCAGTGTG GATCCCAAGA CTGGCAAAGA GACTTACTAT 
GAACTGGAGT CCCTCATCAA CTCTGCCGTG TTCCCAGGCC TTCAGGGGGG TCCTCACAAC 
CATGCCATTG CTGGTGTTGC TGTGGCCCTG AAGCAAGCCA TGACTACAGA ATTCAAAATC 
TACCAGCTCC AGGTGTTGGC CAACTGCAGG GCTCTGTCTG ATGCACTCAC AGAGCTAGGC 
TACAAAATAG TCACAGGTGG CTCTGATAAT CACTTGATCC TAATGGATCT GCGTTCTAAG 
GGTACTGATG GTGGAAGGGC TGAGAAGGTA CTAGAAGCCT GTTCTATCGC CTGCAACAAG 
AATACCTGCC CAGGTGACAA GAGCGCGTTA CGGCCCAGTG GACTGCGACT GGGGACTCCA 
GCATTGACAT CCCGAGGACT TTTGGAAGAA GACTTCCAAA AAGTAGCACA CTTTATCCAC 
AGGGGGATAG AGTTGACACT GCAGATTCAG AGCCACATGG CCACGAAGGC CACACTGAAA 
GAGTTCAAAG AGAAGCTGGC AGGAGATGAG AAGATCCAGA GTGCTGTGGC AACTCTCCGG 
GAAGAAGTGG AGAACTTTGC TTCCAACTTC TCACTACCTG GCCTTCCTGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Nuclear enrichment of folate cofactors and methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protect de novo thymidylate biosynthesis during folate deficiency.
The Journal of biological chemistry289(43)29642-50(2014 Oct)
Field MS,Kamynina E,Agunloye OC,Liebenthal RP,Lamarre SG,Brosnan ME,Brosnan JT,Stover PJ

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Disruption of shmt1 impairs hippocampal neurogenesis and mnemonic function in mice.
The Journal of nutrition143(7)1028-35(2013 Jul)
Abarinov EV,Beaudin AE,Field MS,Perry CA,Allen RH,Stabler SP,Stover PJ

Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
PLoS genetics8(3)e1002573(2012)
Nilsson LM,Forshell TZ,Rimpi S,Kreutzer C,Pretsch W,Bornkamm GW,Nilsson JA

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Shmt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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