Tcf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcf4
Entrez Gene ID	84382
Full Name	transcription factor 4
General protein information	Preferred Names transcription factor 4 Names transcription factor 4 ITF-2 R8f DNA-binding protein RITF-2 SEF-2 SL3-3 enhancer factor 2 TCF-4 immunoglobulin transcription factor 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.1
Summary	transcription factor that acts as an enhancer of the rat tyrosine hydroxylase gene [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053369.1	NP_445821.1	transcription factor 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	TCF4	NP_003190.1
Canis lupus familiaris (dog)	TCF4	NP_001003268.1
	TCF4	XP_001084466.2
Bos taurus (cattle)	TCF4	NP_001029793.1
Rattus norvegicus (Norway rat)	Tcf4	NP_445821.1
Xenopus tropicalis (tropical clawed frog)	tcf4	NP_001096226.2
Mus musculus (house mouse)	Tcf4	NP_038713.1

Gene Ontology
Bibliography

Related articles in PubMed

The Intellectual Disability and Schizophrenia Associated Transcription Factor TCF4 Is Regulated by Neuronal Activity and Protein Kinase A.
Sepp M, Vihma H, Nurm K, Urb M, Page SC, Roots K, Hark A, Maher BJ, Pruunsild P, Timmusk T
The Journal of neuroscience : the official journal of the Society for Neuroscience37(43)10516-10527(2017 Oct)

Expression of Prx1 and Tcf4 is decreased in the diaphragmatic muscle connective tissue of nitrofen-induced congenital diaphragmatic hernia.
Takahashi T, Zimmer J, Friedmacher F, Puri P
Journal of pediatric surgery51(12)1931-1935(2016 Dec)

T-cell factor (TCF/LEF1) binding elements (TBEs) of FasL (Fas ligand or CD95 ligand) bind and cluster Fas (CD95) and form complexes with the TCF-4 and b-catenin transcription factors in vitro and in vivo which result in triggering cell death and/or cell activation.
Liu X, Huang Y, Zhang Y, Li X, Liu C, Huang S, Xu D, Wu Y, Liu X
Cellular and molecular neurobiology36(6)1001-13(2016 Aug)

Psychiatric Risk Gene Transcription Factor 4 Regulates Intrinsic Excitability of Prefrontal Neurons via Repression of SCN10a and KCNQ1.
Rannals MD, Hamersky GR, Page SC, Campbell MN, Briley A, Gallo RA, Phan BN, Hyde TM, Kleinman JE, Shin JH, Jaffe AE, Weinberger DR, Maher BJ
Neuron90(1)43-55(2016 Apr)

TCF4 Mediates the Maintenance of Neuropathic Pain Through Wnt/?-Catenin Signaling Following Peripheral Nerve Injury in Rats.
Xu Z, Chen Y, Yu J, Yin D, Liu C, Chen X, Zhang D
Journal of molecular neuroscience : MN56(2)397-408(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TCF4-controlled transcription in primary cortical neurons is induced by neuronal activity and protein kinase A.
Title: The Intellectual Disability and Schizophrenia Associated Transcription Factor TCF4 Is Regulated by Neuronal Activity and Protein Kinase A.

expression of Prx1 and Tcf4 is decreased in the developing diaphragm in the nitrofen-induced congenital diaphragmatic hernia in Sprague- Dawley rats animal model.
Title: Expression of Prx1 and Tcf4 is decreased in the diaphragmatic muscle connective tissue of nitrofen-induced congenital diaphragmatic hernia.

TCF4 might promote neuronal apoptosis and microglial proliferation after traumatic brain injury
Title: T-cell factor (TCF/LEF1) binding elements (TBEs) of FasL (Fas ligand or CD95 ligand) bind and cluster Fas (CD95) and form complexes with the TCF-4 and b-catenin transcription factors in vitro and in vivo which result in triggering cell death and/or cell activation.

TCF4 is a regulator of neuronal intrinsic excitability in part by repression of Kcnq1 and Scn10a.
Title: Psychiatric Risk Gene Transcription Factor 4 Regulates Intrinsic Excitability of Prefrontal Neurons via Repression of SCN10a and KCNQ1.

Chronic constriction injury induced persistent TCF4 upregulation in the dorsal root ganglion and spinal cord.
Title: TCF4 Mediates the Maintenance of Neuropathic Pain Through Wnt/?-Catenin Signaling Following Peripheral Nerve Injury in Rats.

The following Tcf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09375	NM_053369.1 Latest version!	Rattus norvegicus transcription factor 4 (Tcf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcf4 ( NM_053369.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445821.1 Rattus norvegicus transcription factor 4 (Tcf4), mRNA.

Price & Availability↑

CloneID	ORa09375
Clone ID Related Accession (Same CDS sequence)	NM_053369.1
Accession Version	NM_053369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF149284.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF149284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACTAGCA GGGATCTTGG GTCACATGAC AATCTCTCTC CACCTTTTGC CAATTCCAGA 
ATACAAAGTA AAACAGAAAG GGGTTCATAC TCATCTTATG GGAGAGAAAA CGTACAGGGT 
TGCCACCAGA GTCTCCTCGG AGGGGACATG GATATGGGCA ACCCAGGAAC CCTTTCGCCC 
ACCAAACCTG GCTCCCAGTA CTACCCATAT TCCAGCAATA ACGCCCGACG GAGGCCTCTT 
CACAGTAGTA CCATGGAGGT GCAGACAAAG AAAGTCAGAA AAGTTCCTCC AGGCTTGCCA 
TCTTCAGTCT ACGCTCCTTC AGCCAGCACT GCCGACTACA ACAGGGACTC ACCAGGCTAT 
TCTTCCTCCA AGCCAGCAGC CAGCACTTTC TCCAGCTCCT TCTTCATGCA AGATGGCCAT 
CACAGCAGTG ACCCTTGGAG CTCCTCCAGT GGAATGAATC AGCCCGGCTA TGGAGGGATG 
CTAGGCAACT CTCATATCCC ACAGTCTAGC AGCTACTGTA GCCTGCATCC ACATGAACGT 
CTGAGCTATC CATCGCACTC CTCGGCAGAC ATCAACTCCA GTCTTCCTCC GATGTCCACG 
TTCCATCGAA GTGGCACAAA CCATTACAGC ACCTCTTCCT GCACACCTCC TGCCAACGGA 
ACAGACAGTA TAATGGCAAA CAGAGGAACT GGGGCAGCAG GCAGCTCCCA GACTGGAGAC 
GCTCTGGGGA AAGCCCTGGC TTCGATCTAT TCTCCTGATC ACACGAACAA CAGCTTCTCC 
TCCAACCCTT CAACTCCTGT TGGCTCCCCT CCTTCACTCT CAGCAGGCAC AGCTGTTTGG 
TCTCGAAACG GAGGACAGGC CTCGTCATCT CCCAATTACG AAGGACCCTT GCACTCCTTG 
CAGAGCCGAA TTGAAGACCG TTTAGAAAGA CTGGACGACG CAATTCATGT TCTCCGGAAC 
CACGCGGTGG GCCCATCCAC AGCTGTGCCC GGTGGCCATG GGGACATGCA TGGGATCATC 
GGACCCTCTC ACAATGGAGC AATGGGCAGT CTGGGCTCAG GATATGGAAC TGGCCTTCTC 
TCAGCCAACA GACATTCACT CATGGTCGGG GCCCACCGTG AAGATGGCGT GGCACTGAGA 
GGCAGCCATT CTCTCCTGCC AAACCAGGTT CCGGTTCCAC AGCTTCCGGT CCAGTCTGCA 
ACTTCCCCTG ACTTGAACCC ACCCCAAGAC CCTTACAGAG GGATGCCACC AGGCCTCCAG 
GGACAGAGTG TGTCTTCCGG CAGCTCTGAG ATCAAGTCCG ACGACGAGGG CGATGAGAAC 
CTACAGGACA CAAAATCTTC CGAGGACAAG AAACTAGACG ACGACAAGAA GGATATCAAA 
TCAATTACTA GCAATAACGA TGATGAGGAC CTGACCCCGG AGCAGAAGGC CGAGCGTGAG 
AAGGAGCGGA GGATGGCCAA TAACGCCCGT GAGCGCCTGA GGGTCCGTGA TATCAACGAG 
GCTTTCAAGG AGCTGGGCCG CATGGTACAG CTCCACCTGA AGAGCGACAA GCCCCAGACC 
AAGCTCCTGA TTCTCCACCA GGCAGTGGCT GTCATCCTCA GCCTGGAGCA GCAAGTGCGA 
GAAAGGAACC TGAACCCAAA AGCTGCCTGT CTGAAAAGAA GGGAGGAAGA GAAGGTGTCC 
TCAGAGCCTC CCCCGCTCTC CTTGGCTGGC CCACACCCTG GGATGGGAGA CACAGCGAAT 
CACATGGGTC AGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445821.1
CDS	111..1868
Translation	MTSRDLGSHDNLSPPFANSRIQSKTERGSYSSYGRENVQGCHQSLLGGDMDMGNPGTLSPTKPGSQYYPYSSNNARRRPLHSSTMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYSSSKPAASTFSSSFFMQDGHHSSDPWSSSSGMNQPGYGGMLGNSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGTGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAVPGGHGDMHGIIGPSHNGAMGSLGSGYGTGLLSANRHSLMVGAHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDTANHMGQM

Target ORF information:

RefSeq Version	NM_053369.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transcription factor 4 (Tcf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053369.1

ORF Insert Sequence:

ATGACTAGCA GGGATCTTGG GTCACATGAC AATCTCTCTC CACCTTTTGC CAATTCCAGA 
ATACAAAGTA AAACAGAAAG GGGTTCATAC TCATCTTATG GGAGAGAAAA CGTACAGGGT 
TGCCACCAGA GTCTCCTCGG AGGGGACATG GATATGGGCA ACCCAGGAAC CCTTTCGCCC 
ACCAAACCTG GCTCCCAGTA CTACCCATAT TCCAGCAATA ACGCCCGACG GAGGCCTCTT 
CACAGTAGTA CCATGGAGGT GCAGACAAAG AAAGTCAGAA AAGTTCCTCC AGGCTTGCCA 
TCTTCAGTCT ACGCTCCTTC AGCCAGCACT GCCGACTACA ACAGGGACTC ACCAGGCTAT 
TCTTCCTCCA AGCCAGCAGC CAGCACTTTC TCCAGCTCCT TCTTCATGCA AGATGGCCAT 
CACAGCAGTG ACCCTTGGAG CTCCTCCAGT GGAATGAATC AGCCCGGCTA TGGAGGGATG 
CTAGGCAACT CTCATATCCC ACAGTCTAGC AGCTACTGTA GCCTGCATCC ACATGAACGT 
CTGAGCTATC CATCGCACTC CTCGGCAGAC ATCAACTCCA GTCTTCCTCC GATGTCCACG 
TTCCATCGAA GTGGCACAAA CCATTACAGC ACCTCTTCCT GCACACCTCC TGCCAACGGA 
ACAGACAGTA TAATGGCAAA CAGAGGAACT GGGGCAGCAG GCAGCTCCCA GACTGGAGAC 
GCTCTGGGGA AAGCCCTGGC TTCGATCTAT TCTCCTGATC ACACGAACAA CAGCTTCTCC 
TCCAACCCTT CAACTCCTGT TGGCTCCCCT CCTTCACTCT CAGCAGGCAC AGCTGTTTGG 
TCTCGAAACG GAGGACAGGC CTCGTCATCT CCCAATTACG AAGGACCCTT GCACTCCTTG 
CAGAGCCGAA TTGAAGACCG TTTAGAAAGA CTGGACGACG CAATTCATGT TCTCCGGAAC 
CACGCGGTGG GCCCATCCAC AGCTGTGCCC GGTGGCCATG GGGACATGCA TGGGATCATC 
GGACCCTCTC ACAATGGAGC AATGGGCAGT CTGGGCTCAG GATATGGAAC TGGCCTTCTC 
TCAGCCAACA GACATTCACT CATGGTCGGG GCCCACCGTG AAGATGGCGT GGCACTGAGA 
GGCAGCCATT CTCTCCTGCC AAACCAGGTT CCGGTTCCAC AGCTTCCGGT CCAGTCTGCA 
ACTTCCCCTG ACTTGAACCC ACCCCAAGAC CCTTACAGAG GGATGCCACC AGGCCTCCAG 
GGACAGAGTG TGTCTTCCGG CAGCTCTGAG ATCAAGTCCG ACGACGAGGG CGATGAGAAC 
CTACAGGACA CAAAATCTTC CGAGGACAAG AAACTAGACG ACGACAAGAA GGATATCAAA 
TCAATTACTA GCAATAACGA TGATGAGGAC CTGACCCCGG AGCAGAAGGC CGAGCGTGAG 
AAGGAGCGGA GGATGGCCAA TAACGCCCGT GAGCGCCTGA GGGTCCGTGA TATCAACGAG 
GCTTTCAAGG AGCTGGGCCG CATGGTACAG CTCCACCTGA AGAGCGACAA GCCCCAGACC 
AAGCTCCTGA TTCTCCACCA GGCAGTGGCT GTCATCCTCA GCCTGGAGCA GCAAGTGCGA 
GAAAGGAACC TGAACCCAAA AGCTGCCTGT CTGAAAAGAA GGGAGGAAGA GAAGGTGTCC 
TCAGAGCCTC CCCCGCTCTC CTTGGCTGGC CCACACCCTG GGATGGGAGA CACAGCGAAT 
CACATGGGTC AGATGTGA