Ctsb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctsb
Entrez Gene ID	64529
Full Name	cathepsin B
General protein information	Preferred Names cathepsin B Names cathepsin B RSG-2 cathepsin B1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	lysosomal peptidase that is synthesized as a longer precursor and processed to the mature form [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022597.2	NP_072119.2	cathepsin B preproprotein
XM_017599796.1	XP_017455285.1	cathepsin B isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CTSB	NP_776456.1
Gallus gallus (chicken)	CTSB	XP_004935955.1
Danio rerio (zebrafish)	ctsba	NP_998501.1
Homo sapiens (human)	CTSB	NP_680090.1
Canis lupus familiaris (dog)	CTSB	XP_543203.3
Rattus norvegicus (Norway rat)	Ctsb	NP_072119.2
Arabidopsis thaliana (thale cress)	AT1G02300	NP_563647.1
Oryza sativa (rice)	Os05g0310500	NP_001174338.1
Pan troglodytes (chimpanzee)	CTSB	XP_003317965.1
Xenopus tropicalis (tropical clawed frog)	ctsb	NP_989225.1
Drosophila melanogaster (fruit fly)	CtsB1	NP_572920.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004533	XP_313835.5
Arabidopsis thaliana (thale cress)	AT1G02305	NP_563648.1
	CTSB	XP_002805301.1
Mus musculus (house mouse)	Ctsb	NP_031824.1
Arabidopsis thaliana (thale cress)	AT4G01610	NP_567215.1

Gene Ontology
Bibliography

Related articles in PubMed

Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End-Products (RAGE).
Downs CA, Dang VD, Johnson NM, Denslow ND, Alli AA
Journal of cellular biochemistry119(1)599-606(2018 Jan)

Lysosomal processing of progranulin.
Zhou X, Paushter DH, Feng T, Sun L, Reinheckel T, Hu F
Molecular neurodegeneration12(1)62(2017 08)

Cathepsin B deficiency attenuates cardiac remodeling in response to pressure overload via TNF-?/ASK1/JNK pathway.
Wu QQ, Xu M, Yuan Y, Li FF, Yang Z, Liu Y, Zhou MQ, Bian ZY, Deng W, Gao L, Li H, Tang QZ
American journal of physiology. Heart and circulatory physiology308(9)H1143-54(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CTSB protein functions as a necessary modulator of hypertrophic response by regulating TNF-alpha/ASK1/JNK signaling pathway involved in cardiac remodeling.
Title: Cathepsin B deficiency attenuates cardiac remodeling in response to pressure overload via TNF-?/ASK1/JNK pathway.

Inhibition of cathepsin B in ischemic astrocytes contributes to neuroprotection against cerebral ischemia.
Title: Inhibition of cysteine cathepsin B and L activation in astrocytes contributes to neuroprotection against cerebral ischemia via blocking the tBid-mitochondrial apoptotic signaling pathway.

CTSB contributed to the Atg7-induced NLRP3-dependent proinflammatory response, resulting in aggravation of lipotoxicity, independently of apoptosis in the pancreatic INS-1(823/13) cell line.
Title: Cathepsin B contributes to autophagy-related 7 (Atg7)-induced nod-like receptor 3 (NLRP3)-dependent proinflammatory response and aggravates lipotoxicity in rat insulinoma cell line.

While inhibition of cathepsin D and L failed to protect neurons from OA-induced cell death, CA074-Me, a cathepsin B inhibitor, conferred a protective effect.
Title: CA-074Me, a cathepsin B inhibitor, decreases APP accumulation and protects primary rat cortical neurons treated with okadaic acid.

Results also suggest that increased cathepsin B expression might be associated with NF-kappaB up-regulation, and the exact mechanisms need to be clarified.
Title: Quantitative proteomic study identified cathepsin B associated with doxorubicin-induced damage in H9c2 cardiomyocytes.

The following Ctsb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctsb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06088	NM_022597.2 Latest version!	Rattus norvegicus cathepsin B (Ctsb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa58645	XM_017599796.1 Latest version!	Rattus norvegicus cathepsin B (Ctsb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctsb ( NM_022597.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072119.2 Rattus norvegicus cathepsin B (Ctsb), mRNA.

Price & Availability↑

CloneID	ORa06088
Clone ID Related Accession (Same CDS sequence)	NM_022597.2
Accession Version	NM_022597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	cathepsin B preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB697852.1 and BC072490.1. On Dec 1, 2005 this sequence version replaced NM_022597.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072490.1, X82396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGGTGGT CCTTGATCCC TCTCTCTTGC CTGCTGGCAC TGACCAGTGC CCATGACAAG 
CCTTCCTTTC ACCCACTGTC GGACGACATG ATTAACTATA TCAACAAACA GAATACAACA 
TGGCAGGCTG GACGCAACTT CTACAATGTT GACATAAGCT ATCTGAAGAA GCTGTGTGGA 
ACTGTCCTGG GTGGACCCAA GCTGCCGGAA AGGGTTGGGT TCAGCGAGGA CATAAATCTA 
CCTGAATCCT TTGATGCACG GGAACAGTGG TCCAATTGCC CGACCATCGC ACAGATCAGA 
GACCAGGGGT CCTGTGGCTC TTGTTGGGCA TTTGGGGCAG TGGAAGCCAT GTCTGACCGA 
ATCTGCATTC ACACCAATGG CCGAGTCAAT GTGGAGGTGT CTGCTGAGGA CCTGCTTACC 
TGCTGTGGTA TCCAGTGTGG GGATGGCTGT AATGGTGGCT ATCCCTCTGG AGCATGGAAC 
TTCTGGACTA GAAAAGGCCT GGTTTCTGGT GGAGTATACA ATTCTCATAT AGGCTGCTTA 
CCCTACACCA TCCCTCCCTG TGAACACCAT GTCAATGGCT CCCGTCCCCC ATGCACTGGA 
GAAGGAGATA CTCCCAAGTG CAACAAGATG TGTGAGGCTG GCTACTCCAC ATCCTACAAG 
GAAGATAAGC ACTATGGGTA CACTTCCTAC AGTGTGTCTG ACAGCGAGAA GGAGATCATG 
GCGGAAATCT ACAAAAATGG CCCAGTGGAG GGTGCTTTTA CTGTGTTTTC TGACTTCTTG 
ACTTACAAAT CAGGCGTATA CAAGCATGAA GCCGGTGATG TGATGGGAGG CCATGCCATC 
CGCATTCTGG GCTGGGGAAT AGAGAATGGA GTACCCTACT GGCTGGTAGC AAACTCCTGG 
AACGTTGACT GGGGTGATAA TGGTTTCTTT AAAATCCTCA GAGGAGAGAA CCACTGTGGA 
ATTGAATCAG AAATCGTGGC TGGAATCCCA CGCACTCAGC AGTACTGGGG AAGATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072119.2
CDS	133..1152
Translation	MWWSLIPLSCLLALTSAHDKPSFHPLSDDMINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPERVGFSEDINLPESFDAREQWSNCPTIAQIRDQGSCGSCWAFGAVEAMSDRICIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWNFWTRKGLVSGGVYNSHIGCLPYTIPPCEHHVNGSRPPCTGEGDTPKCNKMCEAGYSTSYKEDKHYGYTSYSVSDSEKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDVMGGHAIRILGWGIENGVPYWLVANSWNVDWGDNGFFKILRGENHCGIESEIVAGIPRTQQYWGRF

Target ORF information:

RefSeq Version	NM_022597.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cathepsin B (Ctsb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022597.2

ORF Insert Sequence:

ATGTGGTGGT CCTTGATCCC TCTCTCTTGC CTGCTGGCAC TGACCAGTGC CCATGACAAG 
CCTTCCTTTC ACCCACTGTC GGACGACATG ATTAACTATA TCAACAAACA GAATACAACA 
TGGCAGGCTG GACGCAACTT CTACAATGTT GACATAAGCT ATCTGAAGAA GCTGTGTGGA 
ACTGTCCTGG GTGGACCCAA GCTGCCGGAA AGGGTTGGGT TCAGCGAGGA CATAAATCTA 
CCTGAATCCT TTGATGCACG GGAACAGTGG TCCAATTGCC CGACCATCGC ACAGATCAGA 
GACCAGGGGT CCTGTGGCTC TTGTTGGGCA TTTGGGGCAG TGGAAGCCAT GTCTGACCGA 
ATCTGCATTC ACACCAATGG CCGAGTCAAT GTGGAGGTGT CTGCTGAGGA CCTGCTTACC 
TGCTGTGGTA TCCAGTGTGG GGATGGCTGT AATGGTGGCT ATCCCTCTGG AGCATGGAAC 
TTCTGGACTA GAAAAGGCCT GGTTTCTGGT GGAGTATACA ATTCTCATAT AGGCTGCTTA 
CCCTACACCA TCCCTCCCTG TGAACACCAT GTCAATGGCT CCCGTCCCCC ATGCACTGGA 
GAAGGAGATA CTCCCAAGTG CAACAAGATG TGTGAGGCTG GCTACTCCAC ATCCTACAAG 
GAAGATAAGC ACTATGGGTA CACTTCCTAC AGTGTGTCTG ACAGCGAGAA GGAGATCATG 
GCGGAAATCT ACAAAAATGG CCCAGTGGAG GGTGCTTTTA CTGTGTTTTC TGACTTCTTG 
ACTTACAAAT CAGGCGTATA CAAGCATGAA GCCGGTGATG TGATGGGAGG CCATGCCATC 
CGCATTCTGG GCTGGGGAAT AGAGAATGGA GTACCCTACT GGCTGGTAGC AAACTCCTGG 
AACGTTGACT GGGGTGATAA TGGTTTCTTT AAAATCCTCA GAGGAGAGAA CCACTGTGGA 
ATTGAATCAG AAATCGTGGC TGGAATCCCA CGCACTCAGC AGTACTGGGG AAGATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctsb ( XM_017599796.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455285.1 Rattus norvegicus cathepsin B (Ctsb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58645
Clone ID Related Accession (Same CDS sequence)	XM_017599796.1
Accession Version	XM_017599796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cathepsin B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGGA ACAGTGGTCC AATTGCCCGA CCATCGCACA GATCAGAGAC CAGGGGTCCT 
GTGGCTCTTG TTGGCTGTAA TGGTGGCTAT CCCTCTGGAG CATGGAACTT CTGGACTAGA 
AAAGGCCTGG TTTCTGGTGG AGTATACAAT TCTCATATAG GCTGCTTACC CTACACCATC 
CCTCCCTGTG AACACCATGT CAATGGCTCC CGTCCCCCAT GCACTGGAGA AGGAGATACT 
CCCAAGTGCA ACAAGATGTG TGAGGCTGGC TACTCCACAT CCTACAAGGA AGATAAGCAC 
TATGGGTACA CTTCCTACAG TGTGTCTGAC AGCGAGAAGG AGATCATGGC GGAAATCTAC 
AAAAATGGCC CAGTGGAGGG TGCTTTTACT GTGTTTTCTG ACTTCTTGAC TTACAAATCA 
GGCGTATACA AGCATGAAGC CGGTGATGTG ATGGGAGGCC ATGCCATCCG CATTCTGGGC 
TGGGGAATAG AGAATGGAGT ACCCTACTGG CTGGTAGCAA ACTCCTGGAA CGTTGACTGG 
GGTGATAATG GTTTCTTTAA AATCCTCAGA GGAGAGAACC ACTGTGGAAT TGAATCAGAA 
ATCGTGGCTG GAATCCCACG CACTCAGCAG TACTGGGGAA GATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455285.1
CDS	1..648
Translation	MHGNSGPIARPSHRSETRGPVALVGCNGGYPSGAWNFWTRKGLVSGGVYNSHIGCLPYTIPPCEHHVNGSRPPCTGEGDTPKCNKMCEAGYSTSYKEDKHYGYTSYSVSDSEKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDVMGGHAIRILGWGIENGVPYWLVANSWNVDWGDNGFFKILRGENHCGIESEIVAGIPRTQQYWGRF

Target ORF information:

RefSeq Version	XM_017599796.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cathepsin B (Ctsb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599796.1

ORF Insert Sequence:

ATGCACGGGA ACAGTGGTCC AATTGCCCGA CCATCGCACA GATCAGAGAC CAGGGGTCCT 
GTGGCTCTTG TTGGCTGTAA TGGTGGCTAT CCCTCTGGAG CATGGAACTT CTGGACTAGA 
AAAGGCCTGG TTTCTGGTGG AGTATACAAT TCTCATATAG GCTGCTTACC CTACACCATC 
CCTCCCTGTG AACACCATGT CAATGGCTCC CGTCCCCCAT GCACTGGAGA AGGAGATACT 
CCCAAGTGCA ACAAGATGTG TGAGGCTGGC TACTCCACAT CCTACAAGGA AGATAAGCAC 
TATGGGTACA CTTCCTACAG TGTGTCTGAC AGCGAGAAGG AGATCATGGC GGAAATCTAC 
AAAAATGGCC CAGTGGAGGG TGCTTTTACT GTGTTTTCTG ACTTCTTGAC TTACAAATCA 
GGCGTATACA AGCATGAAGC CGGTGATGTG ATGGGAGGCC ATGCCATCCG CATTCTGGGC 
TGGGGAATAG AGAATGGAGT ACCCTACTGG CTGGTAGCAA ACTCCTGGAA CGTTGACTGG 
GGTGATAATG GTTTCTTTAA AATCCTCAGA GGAGAGAACC ACTGTGGAAT TGAATCAGAA 
ATCGTGGCTG GAATCCCACG CACTCAGCAG TACTGGGGAA GATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End-Products (RAGE).
Journal of cellular biochemistry119(1)599-606(2018 Jan)
Downs CA,Dang VD,Johnson NM,Denslow ND,Alli AA

Lysosomal processing of progranulin.
Molecular neurodegeneration12(1)62(2017 08)
Zhou X,Paushter DH,Feng T,Sun L,Reinheckel T,Hu F

Cathepsin B deficiency attenuates cardiac remodeling in response to pressure overload via TNF-?/ASK1/JNK pathway.
American journal of physiology. Heart and circulatory physiology308(9)H1143-54(2015 May)
Wu QQ,Xu M,Yuan Y,Li FF,Yang Z,Liu Y,Zhou MQ,Bian ZY,Deng W,Gao L,Li H,Tang QZ

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Ctsb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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