Ltbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ltbp1
Entrez Gene ID	59107
Full Name	latent transforming growth factor beta binding protein 1
General protein information	Preferred Names latent transforming growth factor beta binding protein 1 Names latent-transforming growth factor beta-binding protein 1 LTBP-1 LanC (bacterial lantibiotic synthetase component C)-like 1 TGF-beta-1-BP-1 transforming growth factor beta-1-binding protein 1 transforming growth factor beta-1-masking protein large subunit transforming growth factor-beta (TGF-beta) masking protein large subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q13
Summary	large subunit component of masking protein; neutralizes the activity of TGF-beta 1 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021587.1	NP_067598.1	latent-transforming growth factor beta-binding protein 1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LTBP1	XP_005630374.1
Mus musculus (house mouse)	Ltbp1	NP_064303.2
Gallus gallus (chicken)	LTBP1	XP_004935467.1
Xenopus tropicalis (tropical clawed frog)	ltbp1	XP_002934503.1
Danio rerio (zebrafish)	ltbp1	XP_005158767.1
Homo sapiens (human)	LTBP1	NP_996826.2
Pan troglodytes (chimpanzee)	LTBP1	XP_515398.3
Rattus norvegicus (Norway rat)	Ltbp1	NP_067598.1
Bos taurus (cattle)	LTBP1	NP_001096561.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Proteomics characterization of extracellular space components in the human aorta.
Didangelos A, Yin X, Mandal K, Baumert M, Jahangiri M, Mayr M
Molecular & cellular proteomics : MCP9(9)2048-62(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mesangial expression of LTBP-1 and fibrillin-1 is induced early in experimental nephritis and LTBP-1 and fibrillin-1 are partially colocalized in the nephritic glomerulus.
Title: Induction and coexpression of latent transforming growth factor beta-binding protein-1 and fibrillin-1 in experimental glomerulonephritis.

fibronectin has a role in LTBP1 assembly in the ECM and in regulation of TGF beta via LTBP1 interactions
Title: Fibronectin regulates latent transforming growth factor-beta (TGF beta) by controlling matrix assembly of latent TGF beta-binding protein-1.

LTBP-1 but not TGF-beta1 is induced by PDGF-BB and TGF-beta1 autoinduction does not affect the expression of LTBP-beta1
Title: PDGF-BB induces expression of LTBP-1 but not TGF-beta1 in a rat cirrhotic fat storing cell line.

observations suggest that latent transforming growth factor-beta binding protein-1 plays a critical role in intimal thickening of diabetic artery through the acceleration of smooth muscle cell migration via integrin-beta(3)
Title: Latent transforming growth factor-beta binding protein-1, a component of latent transforming growth factor-beta complex, accelerates the migration of aortic smooth muscle cells in diabetic rats through integrin-beta3.

The following Ltbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ltbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04897	NM_021587.1 Latest version!	Rattus norvegicus latent transforming growth factor beta binding protein 1 (Ltbp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ltbp1 ( NM_021587.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067598.1 Rattus norvegicus latent transforming growth factor beta binding protein 1 (Ltbp1), mRNA.

Price & Availability↑

CloneID	ORa04897
Clone ID Related Accession (Same CDS sequence)	NM_021587.1
Accession Version	NM_021587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Rattus norvegicus(Norway rat)
Product	latent-transforming growth factor beta-binding protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M55431.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M55431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGGAG CCTGGCTCAG GTGGGGACTC CTGCTCTGGG CAGGGCTGCT AGCGTGGTCT 
GCGCACGGCC GGGTGCGGAG GATCACGTAC GTGGTGCGCC CGGGCCCGGG ACTGCCGGCC 
GGAACCCTGC CTCTGGCTGG GCCCCCGCGG ACCTTCAACG TCGCACTGGA CGCCAGGTAC 
AGCCGGAGCT CAACAGCCAC CTCCAGCCGC TCCTTGGCCG GGCCCCCAGC CGAGCGGACC 
CGGCGCACCA GCCAGCCTGG CGGCGCGGCC CTGCCCGGGC TCCGATCTCC GCTGCCACCC 
GAGCCCGCGC GTCCGGGGGC GCCCAGCCGG CAGCTCCACT CAAAGGCCGG TGCTCAGACC 
GCGGTCACTC GCTTCGCCAA ACATGGCAGG CAAGTCGTGC GCTCCAAAGT GCAGCAGGAC 
ACGCAGAGCT CCGGGGGCTC CAGACTGCAG GTTCAGCAGA AGCAGCAGCT GCAGGGGATC 
AATGTCTGTG GAGGGCAGTG CTGCCATGGG TGGAGTAAGG CCCCGGGCTC CCAGAGGTGC 
ACCAAACCAA GCTGTGTTCC ACCATGCCAG AATGGAGGGA TGTGTCTCCG GCCTCAGTTC 
TGTGTGTGTA AGCCAGGGAC AAAGGGCAAA GCCTGCGAGA TCACAGCTGC CCAGGACACC 
ATGTCACCAG TGTTTGGAGG GCAGAACCCT GGCTCCTCGT GGGTCCCTCC TGAGCCAGCA 
GCAAAGCGTA CTTCAACCAA GAAGGCAGAC ACCTTACCAA GGGTCAGTCC AGTGGCCCAG 
ATGACCTTGA CCCTCAAGCC GAAGCCGTCC ATGGGACTCT CCCAGCAGAT ACATTCTCAA 
GTGGCACCCT TGTCTTCCCA GAATGTGATG ATTCGGCATG GCCAGACCCA GGAGTATGTG 
CTCAAGCCCA AGTACTTCCC AGCCCCAAAG GTGGTCTCTG GAGAGCAGTC CACAGAAGGC 
TCTTTCTCTT TAAGATATGG GCAGGAGCAA GGCACAGCAC CCTTTCAGGT GAGTAACCAC 
ACTGGTCGCA TCAAGGTGGT CTTTACTCCG AGCATCTGTA AAGTGACCTG CACCAAGGGC 
AACTGTCACA ACAGCTGCCA GAAGGGGAAT ACCACCACTC TCATTAGTGA GAACGGCCAT 
GCAGCCGACA CCCTGACAGC CACGAACTTC CGAGTGGTAA TTTGCCATCT TCCATGTATG 
AATGGTGGCC AGTGCAGTTC AAGGGACAAA TGTCAGTGCC CTCCAAATTT CACAGGAAAG 
CTTTGCCAGA TCCCTGTCCT TGGTGCCAGT ATGCCTAAAC TCTACCAGCA CGCCCAGCAG 
CCAGGCAAGG CACTGGGGAG TCACGTCATC CATTCCACAC ATACCTTGCC TCTAACCATG 
ACCAACCAGC AAGGGGTCAA AGTGAAATTT CCCCCCAACA TAGTCAATAT CCATGTGAAG 
CATCCTCCTG AGGCTTCTGT GCAGATACAC CAGGTTTCCA GAATTGATGG CCCAGTGGGC 
CAGAGGGTGA AAGAAGTGCA ACCGGGCCAG TCCCAAGTCT CTTACCAAGG GCTGCCCGTC 
CAGAAGACCC AGACAGTCCA TTCCACATAC TCCCACCAGC AGGTCATTCC GCATGTGTAT 
CCCGTGGCTG CTAAGACACA ACTTGGACGA TGCTTCCAGG AAACCATTGG GTCACAGTGT 
GGCAAAGCGC TCCCTGGCCT TTCAAAGCAA GAGGACTGCT GTGGGACAGT GGGGACCTCC 
TGGGGCTTTA ACAAATGCCA GAAATGCCCC AAGAAGCAAT CCTATCATGG ATATACTCAA 
ATGATGGAAT GCCTACAAGG ATATAAACGG GTTAACAACA CCTTTTGTCA AGATATTAAT 
GAATGTCAGC TCCAAGGCGT ATGCCCTAAT GGGGAGTGTT TGAATACCAT GGGCAGCTAC 
AGATGCTCCT GCAAAATGGG ATTTGGGCCT GACCCTACCT TTTCAAGTTG TGTGCCGGAT 
CCCCCTATGA TCTCAGAAGA GAAAGGGCCC TGCTACCGCC TCGTCAGTCC TGGGAGGCAG 
TGTATGCACC CTCTCTCTGT TCACCTCACC AAGCAGATCT GCTGTTGTAG TGTGGGCAAG 
GCCTGGGGCC CACAGTGTGA GAAATGTCCC CTCCCGGGCA CAGCTGCTTT TAAGGAAATC 
TGTCCTGGTG GAATGGGTTA TACGGTTTCT GGCATTCATA GACGCAGGCC AATCCATCAG 
CATATAGGTA AAGAAGCTGT ATTTGTCAAG CCAAAGAACA CTCAACCTGT TGCTAAAAGT 
ACTCATCCTC CACCTCTCCC AGCCAAGGAA GAGCCTGTGG AGGCCCTGAC CTCCTCGCGG 
GAACATGGGC CGGGAGTGGC AGAGCCTGAA GTGGTAACAG CACCTCCTGA GAAGGAAATA 
CCCTCACTGG ATCAAGAGAA AACAAGACTT GAACCTGGGC AACCCCAGCT CTCCCCAGGG 
GTTTCAACTA TTCATCTGCA CCCACAGTTC CCAGTAGTGG TTGAAAAGAC ATCCCCTCCT 
GTGCCTGTGG AGGTGGCTCC TGAAGGTTCT ACATCAAGTG CCAGCCAAGT GATTGCTCCT 
ACGCAAGTAA CAGAAATCAA CGAATGTACT GTGAACCCGG ATATTTGCGG AGCAGGACAC 
TGCATCAACC TGCCGGTGAG GTATACCTGC ATATGCTACG AGGGCTACAA GTTCAGTGAG 
CAGCAGAGGA AGTGCATTGA CATTGATGAA TGTGCTCAGG CCCAGCATCT CTGCTCCCAG 
GGTCGCTGTG AGAACACAGA GGGAAGTTTC CTGTGTATTT GCCCAGCAGG GTTTATAGCC 
AGTGAGGAGG GTTCTAACTG TATAGATGTG GATGAATGTC TGAGGCCCGA TGTGTGTAGG 
GACGGCCGCT GCATCAACAC TGCTGGGGCC TTCCGATGCG AATACTGTGA CAGTGGGTAC 
CGGATGTCAC GACGGGGCCA CTGTGAGGAT ATCGATGAGT GTCTGACCCC AAGTACCTGT 
CCCGAGGAAC AATGCGTGAA TTCCCCAGGT TCTTACCAGT GTGTGCCCTG CACAGAAGGG 
TTCCGTGGCT GGAATGGACA ATGCCTCGAT GTGGACGAGT GCCTGCAGCC AAAGGTCTGT 
ACCAATGGTT CCTGCACCAA CCTGGAAGGC TCCTACATGT GTTCCTGCCA CAAGGGCTAC 
AGCCCCACAC CAGACCATAG ACACTGTCAA GATATTGATG AATGTCAGCA AGGGAACCTG 
TGCATGAACG GGCAGTGCAA AAACACTGAC GGCTCCTTCC GGTGTACCTG TGGGCAGGGC 
TATCAGCTGT CAGCGGCTAA AGACCAATGT GAAGATATTG ACGAATGCGA GCACCGTCAC 
CTCTGCTCTC ACGGGCAGTG CAGGAACACA GAGGGCTCCT TCCAGTGTTT GTGCAACCAG 
GGTTACAGAG CATCTGTGCT TGGAGACCAC TGCGAGGATA TCAATGAATG CTTGGAGGAC 
AGTAGTGTCT GCCAGGGAGG TGACTGCATC AATACAGCAG GGTCCTATGA CTGCACGTGC 
CCGGATGGAC TCCAGCTGAA TGACAATAAG GGCTGTCAAG ACATTAATGA ATGTGCACAG 
CCAGGACTCT GTGCACCTCA TGGGGAGTGT CTAAACACAC AAGGCTCATT CCACTGTGTC 
TGTGAACAAG GGTTCTCCAT CTCTGCAGAT GGTCGTACTT GTGAAGATAT TGATGAGTGT 
GTTAACAACA CTGTGTGTGA CAGTCACGGC TTCTGTGACA ACACAGCCGG CTCTTTCCGC 
TGCCTCTGTT ATCAGGGCTT TCAAGCCCCA CAGGATGGGC AAGGGTGTGT GGATGTGAAC 
GAATGTGAAC TGCTCAGTGG TGTATGTGGG GAGGCTTTCT GTGAAAATGT GGAAGGGTCC 
TTCCTGTGCG TGTGTGCCGA TGAGAACCAG GAGTACAGCC CCATGACTGG GCAGTGTCGC 
TCCCGGGCTA CTGAAGATTC AGGTGTGGAT CGTCAGCCCA AAGAAGAAAA GAAGGAGTGT 
TATTATAATC TCAATGATGC CAGTCTCTGT GATAACGTGC TGGCCCCCAA CGTCACCAAA 
CAAGAGTGCT GCTGTACATC GGGCGCCGGC TGGGGAGACA ATTGTGAGAT CTTCCCTTGC 
CCAGTCCAGG GGACTGCTGA GTTCTCGGAA ATGTGCCCTA GAGGAAAAGG TTTTGTCCCT 
GCTGGAGAAT CCTCTTACGA AACCGGTGGT GAGAACTACA AAGATGCTGA CGAATGCCTG 
CTGTTTGGAG AGGAAATCTG CAAAAACGGT TACTGTTTGA ACACTCAGCC TGGGTATGAA 
TGCTACTGCA AGGAAGGGAC ATACTACGAT CCTGTCAAAT TACAGTGTTT TGATATGGAT 
GAATGCCAAG ACCCTAACAG TTGTATCGAT GGCCAGTGTG TTAATACAGA GGGCTCTTAC 
AACTGCTTTT GCACCCACCC AATGGTCCTG GATGCCTCTG AGAAGAGATG TGTGCAGCCA 
ACTGAATCAA ATGAACAAAT AGAAGAAACC GATGTCTATC AAGATCTGTG CTGGGAGCAT 
CTGAGTGAGG AGTACGTGTG TAGCCGTCCT CTTGTAGGCA AGCAGACGAC ATACACAGAG 
TGCTGCTGTT TGTACGGGGA GGCATGGGGC ATGCAGTGTG CTCTCTGCCC CATGAAGGAC 
TCAGATGACT ATGCCCAGCT GTGCAACATC CCTGTGACAG GACGGCGGCG ACCATATGGA 
CGGGATGCGT TGGTGGACTT CAGTGAACAG TATGGCCCAG AAACAGACCC TTACTTCATT 
CAGGATCGCT TTCTAAACAG CTTTGAGGAG CTACAGGCTG AGGAATGTGG CATCCTCAAC 
GGCTGTGAAA ATGGCCGCTG TGTAAGGGTT CAGGAAGGTT ATACTTGCGA TTGCTTTGAT 
GGATATCATC TGGATATGGC CAAGATGACC TGTGTTGATG TAAATGAATG CAGCGAGCTG 
AATAATCGGA TGTCTCTCTG CAAGAACGCC AAGTGCATTA ACACAGAAGG CTCCTACAAA 
TGCGTGTGTC TACCAGGCTA CGTACCATCT GACAAGCCCA ACTACTGTAC ACCACTGAAC 
ACCGCTTTGA ATTTAGACAA AGACAGTGAC CTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067598.1
CDS	334..5472
Translation	MAGAWLRWGLLLWAGLLAWSAHGRVRRITYVVRPGPGLPAGTLPLAGPPRTFNVALDARYSRSSTATSSRSLAGPPAERTRRTSQPGGAALPGLRSPLPPEPARPGAPSRQLHSKAGAQTAVTRFAKHGRQVVRSKVQQDTQSSGGSRLQVQQKQQLQGINVCGGQCCHGWSKAPGSQRCTKPSCVPPCQNGGMCLRPQFCVCKPGTKGKACEITAAQDTMSPVFGGQNPGSSWVPPEPAAKRTSTKKADTLPRVSPVAQMTLTLKPKPSMGLSQQIHSQVAPLSSQNVMIRHGQTQEYVLKPKYFPAPKVVSGEQSTEGSFSLRYGQEQGTAPFQVSNHTGRIKVVFTPSICKVTCTKGNCHNSCQKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGKLCQIPVLGASMPKLYQHAQQPGKALGSHVIHSTHTLPLTMTNQQGVKVKFPPNIVNIHVKHPPEASVQIHQVSRIDGPVGQRVKEVQPGQSQVSYQGLPVQKTQTVHSTYSHQQVIPHVYPVAAKTQLGRCFQETIGSQCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKQSYHGYTQMMECLQGYKRVNNTFCQDINECQLQGVCPNGECLNTMGSYRCSCKMGFGPDPTFSSCVPDPPMISEEKGPCYRLVSPGRQCMHPLSVHLTKQICCCSVGKAWGPQCEKCPLPGTAAFKEICPGGMGYTVSGIHRRRPIHQHIGKEAVFVKPKNTQPVAKSTHPPPLPAKEEPVEALTSSREHGPGVAEPEVVTAPPEKEIPSLDQEKTRLEPGQPQLSPGVSTIHLHPQFPVVVEKTSPPVPVEVAPEGSTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYKFSEQQRKCIDIDECAQAQHLCSQGRCENTEGSFLCICPAGFIASEEGSNCIDVDECLRPDVCRDGRCINTAGAFRCEYCDSGYRMSRRGHCEDIDECLTPSTCPEEQCVNSPGSYQCVPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHKGYSPTPDHRHCQDIDECQQGNLCMNGQCKNTDGSFRCTCGQGYQLSAAKDQCEDIDECEHRHLCSHGQCRNTEGSFQCLCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGSYDCTCPDGLQLNDNKGCQDINECAQPGLCAPHGECLNTQGSFHCVCEQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRATEDSGVDRQPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFSEMCPRGKGFVPAGESSYETGGENYKDADECLLFGEEICKNGYCLNTQPGYECYCKEGTYYDPVKLQCFDMDECQDPNSCIDGQCVNTEGSYNCFCTHPMVLDASEKRCVQPTESNEQIEETDVYQDLCWEHLSEEYVCSRPLVGKQTTYTECCCLYGEAWGMQCALCPMKDSDDYAQLCNIPVTGRRRPYGRDALVDFSEQYGPETDPYFIQDRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDMAKMTCVDVNECSELNNRMSLCKNAKCINTEGSYKCVCLPGYVPSDKPNYCTPLNTALNLDKDSDLE

Target ORF information:

RefSeq Version	NM_021587.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus latent transforming growth factor beta binding protein 1 (Ltbp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021587.1

ORF Insert Sequence:

ATGGCGGGAG CCTGGCTCAG GTGGGGACTC CTGCTCTGGG CAGGGCTGCT AGCGTGGTCT 
GCGCACGGCC GGGTGCGGAG GATCACGTAC GTGGTGCGCC CGGGCCCGGG ACTGCCGGCC 
GGAACCCTGC CTCTGGCTGG GCCCCCGCGG ACCTTCAACG TCGCACTGGA CGCCAGGTAC 
AGCCGGAGCT CAACAGCCAC CTCCAGCCGC TCCTTGGCCG GGCCCCCAGC CGAGCGGACC 
CGGCGCACCA GCCAGCCTGG CGGCGCGGCC CTGCCCGGGC TCCGATCTCC GCTGCCACCC 
GAGCCCGCGC GTCCGGGGGC GCCCAGCCGG CAGCTCCACT CAAAGGCCGG TGCTCAGACC 
GCGGTCACTC GCTTCGCCAA ACATGGCAGG CAAGTCGTGC GCTCCAAAGT GCAGCAGGAC 
ACGCAGAGCT CCGGGGGCTC CAGACTGCAG GTTCAGCAGA AGCAGCAGCT GCAGGGGATC 
AATGTCTGTG GAGGGCAGTG CTGCCATGGG TGGAGTAAGG CCCCGGGCTC CCAGAGGTGC 
ACCAAACCAA GCTGTGTTCC ACCATGCCAG AATGGAGGGA TGTGTCTCCG GCCTCAGTTC 
TGTGTGTGTA AGCCAGGGAC AAAGGGCAAA GCCTGCGAGA TCACAGCTGC CCAGGACACC 
ATGTCACCAG TGTTTGGAGG GCAGAACCCT GGCTCCTCGT GGGTCCCTCC TGAGCCAGCA 
GCAAAGCGTA CTTCAACCAA GAAGGCAGAC ACCTTACCAA GGGTCAGTCC AGTGGCCCAG 
ATGACCTTGA CCCTCAAGCC GAAGCCGTCC ATGGGACTCT CCCAGCAGAT ACATTCTCAA 
GTGGCACCCT TGTCTTCCCA GAATGTGATG ATTCGGCATG GCCAGACCCA GGAGTATGTG 
CTCAAGCCCA AGTACTTCCC AGCCCCAAAG GTGGTCTCTG GAGAGCAGTC CACAGAAGGC 
TCTTTCTCTT TAAGATATGG GCAGGAGCAA GGCACAGCAC CCTTTCAGGT GAGTAACCAC 
ACTGGTCGCA TCAAGGTGGT CTTTACTCCG AGCATCTGTA AAGTGACCTG CACCAAGGGC 
AACTGTCACA ACAGCTGCCA GAAGGGGAAT ACCACCACTC TCATTAGTGA GAACGGCCAT 
GCAGCCGACA CCCTGACAGC CACGAACTTC CGAGTGGTAA TTTGCCATCT TCCATGTATG 
AATGGTGGCC AGTGCAGTTC AAGGGACAAA TGTCAGTGCC CTCCAAATTT CACAGGAAAG 
CTTTGCCAGA TCCCTGTCCT TGGTGCCAGT ATGCCTAAAC TCTACCAGCA CGCCCAGCAG 
CCAGGCAAGG CACTGGGGAG TCACGTCATC CATTCCACAC ATACCTTGCC TCTAACCATG 
ACCAACCAGC AAGGGGTCAA AGTGAAATTT CCCCCCAACA TAGTCAATAT CCATGTGAAG 
CATCCTCCTG AGGCTTCTGT GCAGATACAC CAGGTTTCCA GAATTGATGG CCCAGTGGGC 
CAGAGGGTGA AAGAAGTGCA ACCGGGCCAG TCCCAAGTCT CTTACCAAGG GCTGCCCGTC 
CAGAAGACCC AGACAGTCCA TTCCACATAC TCCCACCAGC AGGTCATTCC GCATGTGTAT 
CCCGTGGCTG CTAAGACACA ACTTGGACGA TGCTTCCAGG AAACCATTGG GTCACAGTGT 
GGCAAAGCGC TCCCTGGCCT TTCAAAGCAA GAGGACTGCT GTGGGACAGT GGGGACCTCC 
TGGGGCTTTA ACAAATGCCA GAAATGCCCC AAGAAGCAAT CCTATCATGG ATATACTCAA 
ATGATGGAAT GCCTACAAGG ATATAAACGG GTTAACAACA CCTTTTGTCA AGATATTAAT 
GAATGTCAGC TCCAAGGCGT ATGCCCTAAT GGGGAGTGTT TGAATACCAT GGGCAGCTAC 
AGATGCTCCT GCAAAATGGG ATTTGGGCCT GACCCTACCT TTTCAAGTTG TGTGCCGGAT 
CCCCCTATGA TCTCAGAAGA GAAAGGGCCC TGCTACCGCC TCGTCAGTCC TGGGAGGCAG 
TGTATGCACC CTCTCTCTGT TCACCTCACC AAGCAGATCT GCTGTTGTAG TGTGGGCAAG 
GCCTGGGGCC CACAGTGTGA GAAATGTCCC CTCCCGGGCA CAGCTGCTTT TAAGGAAATC 
TGTCCTGGTG GAATGGGTTA TACGGTTTCT GGCATTCATA GACGCAGGCC AATCCATCAG 
CATATAGGTA AAGAAGCTGT ATTTGTCAAG CCAAAGAACA CTCAACCTGT TGCTAAAAGT 
ACTCATCCTC CACCTCTCCC AGCCAAGGAA GAGCCTGTGG AGGCCCTGAC CTCCTCGCGG 
GAACATGGGC CGGGAGTGGC AGAGCCTGAA GTGGTAACAG CACCTCCTGA GAAGGAAATA 
CCCTCACTGG ATCAAGAGAA AACAAGACTT GAACCTGGGC AACCCCAGCT CTCCCCAGGG 
GTTTCAACTA TTCATCTGCA CCCACAGTTC CCAGTAGTGG TTGAAAAGAC ATCCCCTCCT 
GTGCCTGTGG AGGTGGCTCC TGAAGGTTCT ACATCAAGTG CCAGCCAAGT GATTGCTCCT 
ACGCAAGTAA CAGAAATCAA CGAATGTACT GTGAACCCGG ATATTTGCGG AGCAGGACAC 
TGCATCAACC TGCCGGTGAG GTATACCTGC ATATGCTACG AGGGCTACAA GTTCAGTGAG 
CAGCAGAGGA AGTGCATTGA CATTGATGAA TGTGCTCAGG CCCAGCATCT CTGCTCCCAG 
GGTCGCTGTG AGAACACAGA GGGAAGTTTC CTGTGTATTT GCCCAGCAGG GTTTATAGCC 
AGTGAGGAGG GTTCTAACTG TATAGATGTG GATGAATGTC TGAGGCCCGA TGTGTGTAGG 
GACGGCCGCT GCATCAACAC TGCTGGGGCC TTCCGATGCG AATACTGTGA CAGTGGGTAC 
CGGATGTCAC GACGGGGCCA CTGTGAGGAT ATCGATGAGT GTCTGACCCC AAGTACCTGT 
CCCGAGGAAC AATGCGTGAA TTCCCCAGGT TCTTACCAGT GTGTGCCCTG CACAGAAGGG 
TTCCGTGGCT GGAATGGACA ATGCCTCGAT GTGGACGAGT GCCTGCAGCC AAAGGTCTGT 
ACCAATGGTT CCTGCACCAA CCTGGAAGGC TCCTACATGT GTTCCTGCCA CAAGGGCTAC 
AGCCCCACAC CAGACCATAG ACACTGTCAA GATATTGATG AATGTCAGCA AGGGAACCTG 
TGCATGAACG GGCAGTGCAA AAACACTGAC GGCTCCTTCC GGTGTACCTG TGGGCAGGGC 
TATCAGCTGT CAGCGGCTAA AGACCAATGT GAAGATATTG ACGAATGCGA GCACCGTCAC 
CTCTGCTCTC ACGGGCAGTG CAGGAACACA GAGGGCTCCT TCCAGTGTTT GTGCAACCAG 
GGTTACAGAG CATCTGTGCT TGGAGACCAC TGCGAGGATA TCAATGAATG CTTGGAGGAC 
AGTAGTGTCT GCCAGGGAGG TGACTGCATC AATACAGCAG GGTCCTATGA CTGCACGTGC 
CCGGATGGAC TCCAGCTGAA TGACAATAAG GGCTGTCAAG ACATTAATGA ATGTGCACAG 
CCAGGACTCT GTGCACCTCA TGGGGAGTGT CTAAACACAC AAGGCTCATT CCACTGTGTC 
TGTGAACAAG GGTTCTCCAT CTCTGCAGAT GGTCGTACTT GTGAAGATAT TGATGAGTGT 
GTTAACAACA CTGTGTGTGA CAGTCACGGC TTCTGTGACA ACACAGCCGG CTCTTTCCGC 
TGCCTCTGTT ATCAGGGCTT TCAAGCCCCA CAGGATGGGC AAGGGTGTGT GGATGTGAAC 
GAATGTGAAC TGCTCAGTGG TGTATGTGGG GAGGCTTTCT GTGAAAATGT GGAAGGGTCC 
TTCCTGTGCG TGTGTGCCGA TGAGAACCAG GAGTACAGCC CCATGACTGG GCAGTGTCGC 
TCCCGGGCTA CTGAAGATTC AGGTGTGGAT CGTCAGCCCA AAGAAGAAAA GAAGGAGTGT 
TATTATAATC TCAATGATGC CAGTCTCTGT GATAACGTGC TGGCCCCCAA CGTCACCAAA 
CAAGAGTGCT GCTGTACATC GGGCGCCGGC TGGGGAGACA ATTGTGAGAT CTTCCCTTGC 
CCAGTCCAGG GGACTGCTGA GTTCTCGGAA ATGTGCCCTA GAGGAAAAGG TTTTGTCCCT 
GCTGGAGAAT CCTCTTACGA AACCGGTGGT GAGAACTACA AAGATGCTGA CGAATGCCTG 
CTGTTTGGAG AGGAAATCTG CAAAAACGGT TACTGTTTGA ACACTCAGCC TGGGTATGAA 
TGCTACTGCA AGGAAGGGAC ATACTACGAT CCTGTCAAAT TACAGTGTTT TGATATGGAT 
GAATGCCAAG ACCCTAACAG TTGTATCGAT GGCCAGTGTG TTAATACAGA GGGCTCTTAC 
AACTGCTTTT GCACCCACCC AATGGTCCTG GATGCCTCTG AGAAGAGATG TGTGCAGCCA 
ACTGAATCAA ATGAACAAAT AGAAGAAACC GATGTCTATC AAGATCTGTG CTGGGAGCAT 
CTGAGTGAGG AGTACGTGTG TAGCCGTCCT CTTGTAGGCA AGCAGACGAC ATACACAGAG 
TGCTGCTGTT TGTACGGGGA GGCATGGGGC ATGCAGTGTG CTCTCTGCCC CATGAAGGAC 
TCAGATGACT ATGCCCAGCT GTGCAACATC CCTGTGACAG GACGGCGGCG ACCATATGGA 
CGGGATGCGT TGGTGGACTT CAGTGAACAG TATGGCCCAG AAACAGACCC TTACTTCATT 
CAGGATCGCT TTCTAAACAG CTTTGAGGAG CTACAGGCTG AGGAATGTGG CATCCTCAAC 
GGCTGTGAAA ATGGCCGCTG TGTAAGGGTT CAGGAAGGTT ATACTTGCGA TTGCTTTGAT 
GGATATCATC TGGATATGGC CAAGATGACC TGTGTTGATG TAAATGAATG CAGCGAGCTG 
AATAATCGGA TGTCTCTCTG CAAGAACGCC AAGTGCATTA ACACAGAAGG CTCCTACAAA 
TGCGTGTGTC TACCAGGCTA CGTACCATCT GACAAGCCCA ACTACTGTAC ACCACTGAAC 
ACCGCTTTGA ATTTAGACAA AGACAGTGAC CTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Proteomics characterization of extracellular space components in the human aorta.
Molecular & cellular proteomics : MCP9(9)2048-62(2010 Sep)
Didangelos A,Yin X,Mandal K,Baumert M,Jahangiri M,Mayr M

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Ltbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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