Mest cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mest
Entrez Gene ID	58827
Full Name	mesoderm specific transcript
General protein information	Preferred Names mesoderm specific transcript Names mesoderm-specific transcript homolog protein mesoderm specific transcript homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009617.1	NP_001009617.1	mesoderm-specific transcript homolog protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MEST	XP_519382.3
Homo sapiens (human)	MEST	NP_002393.2
Bos taurus (cattle)	MEST	NP_001076837.1
Mus musculus (house mouse)	Mest	NP_032616.1
Rattus norvegicus (Norway rat)	Mest	NP_001009617.1
Gallus gallus (chicken)	MEST	XP_425258.3
	MEST	XP_001097736.1
Canis lupus familiaris (dog)	MEST	XP_848679.1
Xenopus tropicalis (tropical clawed frog)	mest	NP_001005789.1
Danio rerio (zebrafish)	mest	NP_571118.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance.
Nikonova L, Koza RA, Mendoza T, Chao PM, Curley JP, Kozak LP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology22(11)3925-37(2008 Nov)

Retinoic acid receptor gamma activates receptor tyrosine kinase Tie1 gene transcription through transcription factor GATA4 in F9 stem cells.
Su D, Gudas LJ
Experimental hematology36(5)624-41(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mest gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mest cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42333	NM_001009617.1 Latest version!	Rattus norvegicus mesoderm specific transcript (Mest), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mest ( NM_001009617.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009617.1 Rattus norvegicus mesoderm specific transcript (Mest), mRNA.

Price & Availability↑

CloneID	ORa42333
Clone ID Related Accession (Same CDS sequence)	NM_001009617.1
Accession Version	NM_001009617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	mesoderm-specific transcript homolog protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062800.1. ##Evidence-Data-START## Transcript exon combination :: BC062800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCGCC GAGATCGCTT GCGCAGGATG AGGGAGTGGT GGGTCCAAGT AGGGCTCCTG 
GCTGTGCCCT TGCTGGCTGC GTACCTGCAC ATCCCACCCC CTCAGCTCTC CCCTGCTCTG 
CACTCATGGA AGACTTCTGG CAAGTTTTTC ACCTACAAGG GACTACGCAT CTTCTACCAA 
GATTCTGTCG GCGTGGTGGG AAGCCCTGAG ATAGTTGTGC TTTTACATGG CTTTCCGACA 
TCCAGCTATG ACTGGTACAA GATCTGGGAA GGGCTGACCC TGAGGTTCCA TCGAGTGATT 
GCCCTGGATT TCTTAGGCTT TGGCTTCAGT GACAAGCCGA GACCACATCA GTATTCCATA 
TTTGAGCAGG CCAGCATCGT AGAGTCCCTT CTGCGGCACC TGGGGCTCCA GAACCGCAGA 
ATCAACCTAC TGTCTCACGA CTATGGGGAT ATTGTTGCTC AGGAGCTGCT GTACAGGTAC 
AAGCAGAATC GTTCTGGCCG TCTCACCATA AAGAGTCTCT GTCTGTCGAA TGGAGGTATC 
TTTCCTGAGA CCCATCGTCC TCTCCTTCTC CAAAAGCTCC TCAAAGACGG AGGTGTGCTT 
TCACCCATCC TCACCAGGCT CATGAACTTC TTTGTGTTCT CTCGAGGTCT CACTCCAGTC 
TTTGGACCAT ATACTCGACC CACTGAGAGT GAGCTGTGGG ACATGTGGGC TGGGATTCGC 
AACAATGACG GGAACCTGGT CATTGACAGT CTTTTACAGT ACATCAATCA AAGGAAGAAA 
TTTAGAAGAC GCTGGGTGGG AGCACTTGCT TCTGTGACCA TCCCCATTCA TTTTATCTAT 
GGGCCATTGG ATCCTATAAA TCCGTATCCA GAGTTTTTGG AGCTGTACAG GAAAACGCTG 
CCGCGGTCCA CAGTGTCGAT TCTGGATGAC CACATTAGCC ACTACCCACA GCTAGAGGAT 
CCCATGGGCT TCTTGAATGC ATATATGGGC TTCATCAACT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009617.1
CDS	260..1267
Translation	MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKTSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHQYSIFEQASIVESLLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPTESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPINPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF

Target ORF information:

RefSeq Version	NM_001009617.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mesoderm specific transcript (Mest), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009617.1

ORF Insert Sequence:

ATGGTGCGCC GAGATCGCTT GCGCAGGATG AGGGAGTGGT GGGTCCAAGT AGGGCTCCTG 
GCTGTGCCCT TGCTGGCTGC GTACCTGCAC ATCCCACCCC CTCAGCTCTC CCCTGCTCTG 
CACTCATGGA AGACTTCTGG CAAGTTTTTC ACCTACAAGG GACTACGCAT CTTCTACCAA 
GATTCTGTCG GCGTGGTGGG AAGCCCTGAG ATAGTTGTGC TTTTACATGG CTTTCCGACA 
TCCAGCTATG ACTGGTACAA GATCTGGGAA GGGCTGACCC TGAGGTTCCA TCGAGTGATT 
GCCCTGGATT TCTTAGGCTT TGGCTTCAGT GACAAGCCGA GACCACATCA GTATTCCATA 
TTTGAGCAGG CCAGCATCGT AGAGTCCCTT CTGCGGCACC TGGGGCTCCA GAACCGCAGA 
ATCAACCTAC TGTCTCACGA CTATGGGGAT ATTGTTGCTC AGGAGCTGCT GTACAGGTAC 
AAGCAGAATC GTTCTGGCCG TCTCACCATA AAGAGTCTCT GTCTGTCGAA TGGAGGTATC 
TTTCCTGAGA CCCATCGTCC TCTCCTTCTC CAAAAGCTCC TCAAAGACGG AGGTGTGCTT 
TCACCCATCC TCACCAGGCT CATGAACTTC TTTGTGTTCT CTCGAGGTCT CACTCCAGTC 
TTTGGACCAT ATACTCGACC CACTGAGAGT GAGCTGTGGG ACATGTGGGC TGGGATTCGC 
AACAATGACG GGAACCTGGT CATTGACAGT CTTTTACAGT ACATCAATCA AAGGAAGAAA 
TTTAGAAGAC GCTGGGTGGG AGCACTTGCT TCTGTGACCA TCCCCATTCA TTTTATCTAT 
GGGCCATTGG ATCCTATAAA TCCGTATCCA GAGTTTTTGG AGCTGTACAG GAAAACGCTG 
CCGCGGTCCA CAGTGTCGAT TCTGGATGAC CACATTAGCC ACTACCCACA GCTAGAGGAT 
CCCATGGGCT TCTTGAATGC ATATATGGGC TTCATCAACT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology22(11)3925-37(2008 Nov)
Nikonova L,Koza RA,Mendoza T,Chao PM,Curley JP,Kozak LP

Retinoic acid receptor gamma activates receptor tyrosine kinase Tie1 gene transcription through transcription factor GATA4 in F9 stem cells.
Experimental hematology36(5)624-41(2008 May)
Su D,Gudas LJ