Dgkb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgkb
Entrez Gene ID	54248
Full Name	diacylglycerol kinase, beta
Synonyms	Dagk
General protein information	Preferred Names diacylglycerol kinase, beta Names diacylglycerol kinase beta 90 kDa diacylglycerol kinase DAG kinase beta DGK-beta Diacylglycerol kinase 90kDa diacylglycerol kinase beta, 90kDa diglyceride kinase beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q21
Summary	beta isotype of the diacylglycerol kinase family, regulates intracellular concentrations of the second messenger diacylglycerol (DAG) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594328.1	XP_017449817.1	diacylglycerol kinase beta isoform X1
XM_017594330.1	XP_017449819.1	diacylglycerol kinase beta isoform X2
XM_017594331.1	XP_017449820.1	diacylglycerol kinase beta isoform X3
NM_019304.1	NP_062177.1	diacylglycerol kinase beta
XM_008764649.2	XP_008762871.2	diacylglycerol kinase beta isoform X1
XM_017594332.1	XP_017449821.1	diacylglycerol kinase beta isoform X4
XM_017594329.1	XP_017449818.1	diacylglycerol kinase beta isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DGKB	NP_004071.1
Bos taurus (cattle)	DGKB	NP_001179827.1
Drosophila melanogaster (fruit fly)	Dgk	NP_001097220.1
Canis lupus familiaris (dog)	DGKB	XP_539445.3
Danio rerio (zebrafish)	dgkb	XP_003200112.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006762	XP_308982.4
Caenorhabditis elegans (roundworm)	dgk-3	NP_499031.3
Xenopus tropicalis (tropical clawed frog)	dgkb	NP_001096214.1
Pan troglodytes (chimpanzee)	DGKB	XP_001148960.1
	DGKB	XP_001105384.1
Rattus norvegicus (Norway rat)	Dgkb	NP_062177.1
Gallus gallus (chicken)	DGKB	XP_001235516.3
Mus musculus (house mouse)	Dgkb	NP_848796.2

Gene Ontology
Bibliography

Related articles in PubMed

Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.
Sekerkov? G, Watanabe M, Martina M, Mugnaini E
Brain structure & function219(2)719-49(2014 Mar)

Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.
Hozumi Y, Matsui H, Sakane F, Watanabe M, Goto K
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society61(6)462-76(2013 Jun)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Gellett AM, Kharel Y, Sunkara M, Morris AJ, Lynch KR
Biochemical and biophysical research communications422(4)758-63(2012 Jun)

Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function.
Shirai Y, Kouzuki T, Kakefuda K, Moriguchi S, Oyagi A, Horie K, Morita SY, Shimazawa M, Fukunaga K, Takeda J, Saito N, Hara H
PloS one5(7)e11602(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonistrated that dgkb distribuate in rat cerebellum.
Title: Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.

The DGKbeta localize in the outer plexiform layer, within which photoreceptor cells make contact with bipolar and horizontal cells.
Title: Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.

functional roles of DGK in the pituitary intermediate lobe
Title: Signaling cascade of diacylglycerol kinase ? in the pituitary intermediate lobe: dopamine D2 receptor/phospholipase C?4/diacylglycerol kinase ?/protein kinase C?.

In the hippocampus, DGKbeta is expressed in both projection neurons and interneurons and is accumulated at the perisynapse of dendritic spines in asymmetrical synapses. It may be involved in dendrite outgrowth and spinogenesis.
Title: Diacylglycerol kinase beta promotes dendritic outgrowth and spine maturation in developing hippocampal neurons.

Data suggest that abundant diacylglycerol kinase beta is present perisynaptically in medium spiny neurons so that it can effectively produce phosphatidic acid upon activation of Gq-coupled receptors and modulate striatal output neurons.
Title: Diacylglycerol kinase beta accumulates on the perisynaptic site of medium spiny neurons in the striatum.

The following Dgkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42718	NM_019304.1 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa61732	XM_017594328.1 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa61732	XM_008764649.2 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa61733	XM_017594329.1 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa61733	XM_017594330.1 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa61734	XM_017594331.1 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa61735	XM_017594332.1 Latest version!	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgkb ( NM_019304.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062177.1 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), mRNA.

Price & Availability↑

CloneID	ORa42718
Clone ID Related Accession (Same CDS sequence)	NM_019304.1
Accession Version	NM_019304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D16100.1. On Jan 12, 2005 this sequence version replaced XM_346768.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D16100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTTCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGTGCCAG AGCCCCGCCC TCCTGCATCA AGACCTATGT GAAGTCCAAG 
AAGAACACTG ACGTCATGCA CCATTATTGG GTCGAAGGCA ATTGCCCAAC CAAGTGTGAT 
AAGTGCCACA AGACAGTGAA ATGTTACCAG GGCCTGACGG GGCTGCACTG TGTTTGGTGT 
CAGACCACGC TGCACAATAA ATGTGCTTCT CATCTGAAGC CTGAATGTGA CTGTGGACCC 
TTGAAGGACC ATATTTTGCC ACCCACCACA ATCTGTCCAG TTGTATTGAC TATGCCCACT 
GCCGGAACTT CAGTTCCTGA GGAAAGACAG TCAACAGCTA AAAAGGAAAA GGGCAGCTCC 
CAGCAGCCAA ATAAAGTGAC AGACAAGAAC AAAATGCAAA GAGCTAACTC TGTCACTATG 
GATGGACAAG GTTTGCAGAT CACTCCTATT CCTGGCACAC ATCCTCTTCT AGTTTTTGTC 
AACCCTAAAA GTGGTGGGAA GCAAGGAGAA CGAATTTACA GAAAATTCCA GTATCTTCTA 
AATCCTCGTC AGGTTTACAG TCTTTCTGGA AATGGACCAA TGCCAGGGTT ACATTTTTTT 
CGCGATGTCC CTGACTTCAG AGTGTTAGCA TGCGGTGGAG ATGGGACTGT GGGCTGGATT 
TTGGACTGCA TAGAAAAGGC CAATGTAGTC AAGCACCCTC CAGTTGCTAT CCTGCCTCTT 
GGGACGGGCA ATGACCTAGC AAGATGCCTG CGGTGGGGTG GAGGTTATGA AGGTGAAAAT 
CTTATGAAGA TCCTAAAAGA CATTGAAAGC AGCACAGAAA TCATGTTGGA CAGGTGGAAG 
TTTGAAGTCA CACCCAACGA CAAAGACGAG AAAGGAGACC CTGTGCCTTA CAGCATCATC 
AACAACTACT TTTCCATTGG CGTGGATGCT TCCATTGCAC ACAGATTCCA CATCATGAGA 
GAAAAGCACC CGGAGAAATT CAACAGCCGA ATGAAGAACA AATTTTGGTA CTTTGAGTTT 
GGCACATCAG AAACCTTCTC AGCCACCTGC AAGAAACTGC ATGAATCTGT AGAAATAGAA 
TGTGATGGAG TACAGATAGA TTTGATAAAC ATCTCTCTGC AAGGAATTGC AATTTTGAAT 
ATACCAAGCA TGCATGGAGG ATCTAATCTT TGGGGAGAAT CTAAAAAGAA AAGAAGTCAT 
CGACGGATAG AGAAAAAAGG CTCTGACAAA AGACCCACTC TCACAGATGC AAAAGAACTG 
AAGTTTGCAA GTCAAGATCT GAGTGACCAG CTACTGGAGG TTGTTGGCTT GGAAGGAGCA 
ATGGAAATGG GCCAAATATA CACGGGGCTG AAAAGTGCTG GCCGGCGGCT GGCTCAGTGC 
TCCTCTGTGG TTATCAGGAC AAGCAAGTCA CTGCCAATGC AGATTGATGG AGAGCCATGG 
ATGCAGACCC CATGCACAAT AAAAATTACA CACAAGAACC AGGCCCCAAT GCTGATGGGA 
CCTCCACCTA AAACTGGATT ATTCTGCTCC CTCATCAAAA GAACAAGAAA CCGAAGCAAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062177.1
CDS	286..2691
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCFPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLQGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	NM_019304.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019304.1

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTTCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGTGCCAG AGCCCCGCCC TCCTGCATCA AGACCTATGT GAAGTCCAAG 
AAGAACACTG ACGTCATGCA CCATTATTGG GTCGAAGGCA ATTGCCCAAC CAAGTGTGAT 
AAGTGCCACA AGACAGTGAA ATGTTACCAG GGCCTGACGG GGCTGCACTG TGTTTGGTGT 
CAGACCACGC TGCACAATAA ATGTGCTTCT CATCTGAAGC CTGAATGTGA CTGTGGACCC 
TTGAAGGACC ATATTTTGCC ACCCACCACA ATCTGTCCAG TTGTATTGAC TATGCCCACT 
GCCGGAACTT CAGTTCCTGA GGAAAGACAG TCAACAGCTA AAAAGGAAAA GGGCAGCTCC 
CAGCAGCCAA ATAAAGTGAC AGACAAGAAC AAAATGCAAA GAGCTAACTC TGTCACTATG 
GATGGACAAG GTTTGCAGAT CACTCCTATT CCTGGCACAC ATCCTCTTCT AGTTTTTGTC 
AACCCTAAAA GTGGTGGGAA GCAAGGAGAA CGAATTTACA GAAAATTCCA GTATCTTCTA 
AATCCTCGTC AGGTTTACAG TCTTTCTGGA AATGGACCAA TGCCAGGGTT ACATTTTTTT 
CGCGATGTCC CTGACTTCAG AGTGTTAGCA TGCGGTGGAG ATGGGACTGT GGGCTGGATT 
TTGGACTGCA TAGAAAAGGC CAATGTAGTC AAGCACCCTC CAGTTGCTAT CCTGCCTCTT 
GGGACGGGCA ATGACCTAGC AAGATGCCTG CGGTGGGGTG GAGGTTATGA AGGTGAAAAT 
CTTATGAAGA TCCTAAAAGA CATTGAAAGC AGCACAGAAA TCATGTTGGA CAGGTGGAAG 
TTTGAAGTCA CACCCAACGA CAAAGACGAG AAAGGAGACC CTGTGCCTTA CAGCATCATC 
AACAACTACT TTTCCATTGG CGTGGATGCT TCCATTGCAC ACAGATTCCA CATCATGAGA 
GAAAAGCACC CGGAGAAATT CAACAGCCGA ATGAAGAACA AATTTTGGTA CTTTGAGTTT 
GGCACATCAG AAACCTTCTC AGCCACCTGC AAGAAACTGC ATGAATCTGT AGAAATAGAA 
TGTGATGGAG TACAGATAGA TTTGATAAAC ATCTCTCTGC AAGGAATTGC AATTTTGAAT 
ATACCAAGCA TGCATGGAGG ATCTAATCTT TGGGGAGAAT CTAAAAAGAA AAGAAGTCAT 
CGACGGATAG AGAAAAAAGG CTCTGACAAA AGACCCACTC TCACAGATGC AAAAGAACTG 
AAGTTTGCAA GTCAAGATCT GAGTGACCAG CTACTGGAGG TTGTTGGCTT GGAAGGAGCA 
ATGGAAATGG GCCAAATATA CACGGGGCTG AAAAGTGCTG GCCGGCGGCT GGCTCAGTGC 
TCCTCTGTGG TTATCAGGAC AAGCAAGTCA CTGCCAATGC AGATTGATGG AGAGCCATGG 
ATGCAGACCC CATGCACAAT AAAAATTACA CACAAGAACC AGGCCCCAAT GCTGATGGGA 
CCTCCACCTA AAACTGGATT ATTCTGCTCC CTCATCAAAA GAACAAGAAA CCGAAGCAAG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkb ( XM_017594328.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449817.1 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61732
Clone ID Related Accession (Same CDS sequence)	XM_017594328.1 , XM_008764649.2
Accession Version	XM_017594328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTCCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGCGTGGC CAGAGCCCCG CCCTCCTGCA TCAAGACCTA TGTGAAGTCC 
AAGAAGAACA CTGACGTCAT GCACCATTAT TGGGTCGAAG GCAATTGCCC AACCAAGTGT 
GATAAGTGCC ACAAGACAGT GAAATGTTAC CAGGGCCTGA CGGGGCTGCA CTGTGTTTGG 
TGTCAGACCA CGCTGCACAA TAAATGTGCT TCTCATCTGA AGCCTGAATG TGACTGTGGA 
CCCTTGAAGG ACCATATTTT GCCACCCACC ACAATCTGTC CAGTTGTATT GCAGACTATG 
CCCACTGCCG GAACTTCAGT TCCTGAGGAA AGACAGTCAA CAGCTAAAAA GGAAAAGGGC 
AGCTCCCAGC AGCCAAATAA AGTGACAGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTC 
ACTATGGATG GACAAGGTTT GCAGATCACT CCTATTCCTG GCACACATCC TCTTCTAGTT 
TTTGTCAACC CTAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAGTAT 
CTTCTAAATC CTCGTCAGGT TTACAGTCTT TCTGGAAATG GACCAATGCC AGGGTTACAT 
TTTTTTCGCG ATGTCCCTGA CTTCAGAGTG TTAGCATGCG GTGGAGATGG GACTGTGGGC 
TGGATTTTGG ACTGCATAGA AAAGGCCAAT GTAGTCAAGC ACCCTCCAGT TGCTATCCTG 
CCTCTTGGGA CGGGCAATGA CCTAGCAAGA TGCCTGCGGT GGGGTGGAGG TTATGAAGGT 
GAAAATCTTA TGAAGATCCT AAAAGACATT GAAAGCAGCA CAGAAATCAT GTTGGACAGG 
TGGAAGTTTG AAGTCACACC CAACGACAAA GACGAGAAAG GAGACCCTGT GCCTTACAGC 
ATCATCAACA ACTACTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCACATC 
ATGAGAGAAA AGCACCCGGA GAAATTCAAC AGCCGAATGA AGAACAAATT TTGGTACTTT 
GAGTTTGGCA CATCAGAAAC CTTCTCAGCC ACCTGCAAGA AACTGCATGA ATCTGTAGAA 
ATAGAATGTG ATGGAGTACA GATAGATTTG ATAAACATCT CTCTGGAAGG AATTGCAATT 
TTGAATATAC CAAGCATGCA TGGAGGATCT AATCTTTGGG GAGAATCTAA AAAGAAAAGA 
AGTCATCGAC GGATAGAGAA AAAAGGCTCT GACAAAAGAC CCACTCTCAC AGATGCAAAA 
GAACTGAAGT TTGCAAGTCA AGATCTGAGT GACCAGCTAC TGGAGGTTGT TGGCTTGGAA 
GGAGCAATGG AAATGGGCCA AATATACACG GGGCTGAAAA GTGCTGGCCG GCGGCTGGCT 
CAGTGCTCCT CTGTGGTTAT CAGGACAAGC AAGTCACTGC CAATGCAGAT TGATGGAGAG 
CCATGGATGC AGACCCCATG CACAATAAAA ATTACACACA AGAACCAGGC CCCAATGCTG 
ATGGGACCTC CACCTAAAAC TGGATTATTC TGCTCCCTCA TCAAAAGAAC AAGAAACCGA 
AGCAAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449817.1
CDS	411..2822
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	XM_017594328.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594328.1

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTCCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGCGTGGC CAGAGCCCCG CCCTCCTGCA TCAAGACCTA TGTGAAGTCC 
AAGAAGAACA CTGACGTCAT GCACCATTAT TGGGTCGAAG GCAATTGCCC AACCAAGTGT 
GATAAGTGCC ACAAGACAGT GAAATGTTAC CAGGGCCTGA CGGGGCTGCA CTGTGTTTGG 
TGTCAGACCA CGCTGCACAA TAAATGTGCT TCTCATCTGA AGCCTGAATG TGACTGTGGA 
CCCTTGAAGG ACCATATTTT GCCACCCACC ACAATCTGTC CAGTTGTATT GCAGACTATG 
CCCACTGCCG GAACTTCAGT TCCTGAGGAA AGACAGTCAA CAGCTAAAAA GGAAAAGGGC 
AGCTCCCAGC AGCCAAATAA AGTGACAGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTC 
ACTATGGATG GACAAGGTTT GCAGATCACT CCTATTCCTG GCACACATCC TCTTCTAGTT 
TTTGTCAACC CTAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAGTAT 
CTTCTAAATC CTCGTCAGGT TTACAGTCTT TCTGGAAATG GACCAATGCC AGGGTTACAT 
TTTTTTCGCG ATGTCCCTGA CTTCAGAGTG TTAGCATGCG GTGGAGATGG GACTGTGGGC 
TGGATTTTGG ACTGCATAGA AAAGGCCAAT GTAGTCAAGC ACCCTCCAGT TGCTATCCTG 
CCTCTTGGGA CGGGCAATGA CCTAGCAAGA TGCCTGCGGT GGGGTGGAGG TTATGAAGGT 
GAAAATCTTA TGAAGATCCT AAAAGACATT GAAAGCAGCA CAGAAATCAT GTTGGACAGG 
TGGAAGTTTG AAGTCACACC CAACGACAAA GACGAGAAAG GAGACCCTGT GCCTTACAGC 
ATCATCAACA ACTACTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCACATC 
ATGAGAGAAA AGCACCCGGA GAAATTCAAC AGCCGAATGA AGAACAAATT TTGGTACTTT 
GAGTTTGGCA CATCAGAAAC CTTCTCAGCC ACCTGCAAGA AACTGCATGA ATCTGTAGAA 
ATAGAATGTG ATGGAGTACA GATAGATTTG ATAAACATCT CTCTGGAAGG AATTGCAATT 
TTGAATATAC CAAGCATGCA TGGAGGATCT AATCTTTGGG GAGAATCTAA AAAGAAAAGA 
AGTCATCGAC GGATAGAGAA AAAAGGCTCT GACAAAAGAC CCACTCTCAC AGATGCAAAA 
GAACTGAAGT TTGCAAGTCA AGATCTGAGT GACCAGCTAC TGGAGGTTGT TGGCTTGGAA 
GGAGCAATGG AAATGGGCCA AATATACACG GGGCTGAAAA GTGCTGGCCG GCGGCTGGCT 
CAGTGCTCCT CTGTGGTTAT CAGGACAAGC AAGTCACTGC CAATGCAGAT TGATGGAGAG 
CCATGGATGC AGACCCCATG CACAATAAAA ATTACACACA AGAACCAGGC CCCAATGCTG 
ATGGGACCTC CACCTAAAAC TGGATTATTC TGCTCCCTCA TCAAAAGAAC AAGAAACCGA 
AGCAAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkb ( XM_008764649.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762871.2 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61732
Clone ID Related Accession (Same CDS sequence)	XM_017594328.1 , XM_008764649.2
Accession Version	XM_008764649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTCCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGCGTGGC CAGAGCCCCG CCCTCCTGCA TCAAGACCTA TGTGAAGTCC 
AAGAAGAACA CTGACGTCAT GCACCATTAT TGGGTCGAAG GCAATTGCCC AACCAAGTGT 
GATAAGTGCC ACAAGACAGT GAAATGTTAC CAGGGCCTGA CGGGGCTGCA CTGTGTTTGG 
TGTCAGACCA CGCTGCACAA TAAATGTGCT TCTCATCTGA AGCCTGAATG TGACTGTGGA 
CCCTTGAAGG ACCATATTTT GCCACCCACC ACAATCTGTC CAGTTGTATT GCAGACTATG 
CCCACTGCCG GAACTTCAGT TCCTGAGGAA AGACAGTCAA CAGCTAAAAA GGAAAAGGGC 
AGCTCCCAGC AGCCAAATAA AGTGACAGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTC 
ACTATGGATG GACAAGGTTT GCAGATCACT CCTATTCCTG GCACACATCC TCTTCTAGTT 
TTTGTCAACC CTAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAGTAT 
CTTCTAAATC CTCGTCAGGT TTACAGTCTT TCTGGAAATG GACCAATGCC AGGGTTACAT 
TTTTTTCGCG ATGTCCCTGA CTTCAGAGTG TTAGCATGCG GTGGAGATGG GACTGTGGGC 
TGGATTTTGG ACTGCATAGA AAAGGCCAAT GTAGTCAAGC ACCCTCCAGT TGCTATCCTG 
CCTCTTGGGA CGGGCAATGA CCTAGCAAGA TGCCTGCGGT GGGGTGGAGG TTATGAAGGT 
GAAAATCTTA TGAAGATCCT AAAAGACATT GAAAGCAGCA CAGAAATCAT GTTGGACAGG 
TGGAAGTTTG AAGTCACACC CAACGACAAA GACGAGAAAG GAGACCCTGT GCCTTACAGC 
ATCATCAACA ACTACTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCACATC 
ATGAGAGAAA AGCACCCGGA GAAATTCAAC AGCCGAATGA AGAACAAATT TTGGTACTTT 
GAGTTTGGCA CATCAGAAAC CTTCTCAGCC ACCTGCAAGA AACTGCATGA ATCTGTAGAA 
ATAGAATGTG ATGGAGTACA GATAGATTTG ATAAACATCT CTCTGGAAGG AATTGCAATT 
TTGAATATAC CAAGCATGCA TGGAGGATCT AATCTTTGGG GAGAATCTAA AAAGAAAAGA 
AGTCATCGAC GGATAGAGAA AAAAGGCTCT GACAAAAGAC CCACTCTCAC AGATGCAAAA 
GAACTGAAGT TTGCAAGTCA AGATCTGAGT GACCAGCTAC TGGAGGTTGT TGGCTTGGAA 
GGAGCAATGG AAATGGGCCA AATATACACG GGGCTGAAAA GTGCTGGCCG GCGGCTGGCT 
CAGTGCTCCT CTGTGGTTAT CAGGACAAGC AAGTCACTGC CAATGCAGAT TGATGGAGAG 
CCATGGATGC AGACCCCATG CACAATAAAA ATTACACACA AGAACCAGGC CCCAATGCTG 
ATGGGACCTC CACCTAAAAC TGGATTATTC TGCTCCCTCA TCAAAAGAAC AAGAAACCGA 
AGCAAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762871.2
CDS	260..2671
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	XM_008764649.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764649.2

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTCCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGCGTGGC CAGAGCCCCG CCCTCCTGCA TCAAGACCTA TGTGAAGTCC 
AAGAAGAACA CTGACGTCAT GCACCATTAT TGGGTCGAAG GCAATTGCCC AACCAAGTGT 
GATAAGTGCC ACAAGACAGT GAAATGTTAC CAGGGCCTGA CGGGGCTGCA CTGTGTTTGG 
TGTCAGACCA CGCTGCACAA TAAATGTGCT TCTCATCTGA AGCCTGAATG TGACTGTGGA 
CCCTTGAAGG ACCATATTTT GCCACCCACC ACAATCTGTC CAGTTGTATT GCAGACTATG 
CCCACTGCCG GAACTTCAGT TCCTGAGGAA AGACAGTCAA CAGCTAAAAA GGAAAAGGGC 
AGCTCCCAGC AGCCAAATAA AGTGACAGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTC 
ACTATGGATG GACAAGGTTT GCAGATCACT CCTATTCCTG GCACACATCC TCTTCTAGTT 
TTTGTCAACC CTAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAGTAT 
CTTCTAAATC CTCGTCAGGT TTACAGTCTT TCTGGAAATG GACCAATGCC AGGGTTACAT 
TTTTTTCGCG ATGTCCCTGA CTTCAGAGTG TTAGCATGCG GTGGAGATGG GACTGTGGGC 
TGGATTTTGG ACTGCATAGA AAAGGCCAAT GTAGTCAAGC ACCCTCCAGT TGCTATCCTG 
CCTCTTGGGA CGGGCAATGA CCTAGCAAGA TGCCTGCGGT GGGGTGGAGG TTATGAAGGT 
GAAAATCTTA TGAAGATCCT AAAAGACATT GAAAGCAGCA CAGAAATCAT GTTGGACAGG 
TGGAAGTTTG AAGTCACACC CAACGACAAA GACGAGAAAG GAGACCCTGT GCCTTACAGC 
ATCATCAACA ACTACTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCACATC 
ATGAGAGAAA AGCACCCGGA GAAATTCAAC AGCCGAATGA AGAACAAATT TTGGTACTTT 
GAGTTTGGCA CATCAGAAAC CTTCTCAGCC ACCTGCAAGA AACTGCATGA ATCTGTAGAA 
ATAGAATGTG ATGGAGTACA GATAGATTTG ATAAACATCT CTCTGGAAGG AATTGCAATT 
TTGAATATAC CAAGCATGCA TGGAGGATCT AATCTTTGGG GAGAATCTAA AAAGAAAAGA 
AGTCATCGAC GGATAGAGAA AAAAGGCTCT GACAAAAGAC CCACTCTCAC AGATGCAAAA 
GAACTGAAGT TTGCAAGTCA AGATCTGAGT GACCAGCTAC TGGAGGTTGT TGGCTTGGAA 
GGAGCAATGG AAATGGGCCA AATATACACG GGGCTGAAAA GTGCTGGCCG GCGGCTGGCT 
CAGTGCTCCT CTGTGGTTAT CAGGACAAGC AAGTCACTGC CAATGCAGAT TGATGGAGAG 
CCATGGATGC AGACCCCATG CACAATAAAA ATTACACACA AGAACCAGGC CCCAATGCTG 
ATGGGACCTC CACCTAAAAC TGGATTATTC TGCTCCCTCA TCAAAAGAAC AAGAAACCGA 
AGCAAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkb ( XM_017594330.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449819.1 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa61733
Clone ID Related Accession (Same CDS sequence)	XM_017594330.1 , XM_017594329.1
Accession Version	XM_017594330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGACC ATAGATTTTG AAGGATTCAA GCTGTTCATG 
AAGACCTTCC TGGAAGCTGA GCTCCCTGAC GATTTCACTG CCCACCTTTT CATGTCATTC 
AGCAACAAAT TCCCCCATTC CAGCCCCAAC GTAAAGAGCA AGCCAGCTCT GCTTTCAGGT 
GGTTTGAGGA TGAATAAGGG TGCCATCACC CCTCCACGTT CTTCTCCAGC AAATACGTGC 
TCCCCGGAAG TGATCCACCT CAAGGATATT GTCTGTTACC TGTCTCTTCT GGAAAGAGGA 
AGACCTGAGG ACAAGCTTGA GTTTATGTTT CGCCTGTATG ACACCGATGG GAATGGCTTC 
CTGGACAGCT CGGAATTAGA AAATATCATC GGTCAGATGA TGCATGTTGC CGAGTACCTG 
GAATGGGATG TTACTGAACT TAATCCGATC CTTCATGAAA TGATGGAAGA AATCGACTAT 
GACCGTGACG GCACTGTGTC TCTGGAAGAG TGGATTCAAG GAGGAATGAC GACTATCCCA 
CTTCTAGTCT TGCTGGGCTT GGAAAACAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA 
CTCAAGCACT TTAACAAGCC CGCCTACTGC AATCTCTGCC TGAACATGCT GATTGGCGTG 
GGGAAGCAGG GCCTCTGCTG CTCCTTCTGC AAGTACACCG TCCATGAGCG CTGCGTGGCC 
AGAGCCCCGC CCTCCTGCAT CAAGACCTAT GTGAAGTCCA AGAAGAACAC TGACGTCATG 
CACCATTATT GGGTCGAAGG CAATTGCCCA ACCAAGTGTG ATAAGTGCCA CAAGACAGTG 
AAATGTTACC AGGGCCTGAC GGGGCTGCAC TGTGTTTGGT GTCAGACCAC GCTGCACAAT 
AAATGTGCTT CTCATCTGAA GCCTGAATGT GACTGTGGAC CCTTGAAGGA CCATATTTTG 
CCACCCACCA CAATCTGTCC AGTTGTATTG CAGACTATGC CCACTGCCGG AACTTCAGTT 
CCTGAGGAAA GACAGTCAAC AGCTAAAAAG GAAAAGGGCA GCTCCCAGCA GCCAAATAAA 
GTGACAGACA AGAACAAAAT GCAAAGAGCT AACTCTGTCA CTATGGATGG ACAAGGTTTG 
CAGATCACTC CTATTCCTGG CACACATCCT CTTCTAGTTT TTGTCAACCC TAAAAGTGGT 
GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAGTATC TTCTAAATCC TCGTCAGGTT 
TACAGTCTTT CTGGAAATGG ACCAATGCCA GGGTTACATT TTTTTCGCGA TGTCCCTGAC 
TTCAGAGTGT TAGCATGCGG TGGAGATGGG ACTGTGGGCT GGATTTTGGA CTGCATAGAA 
AAGGCCAATG TAGTCAAGCA CCCTCCAGTT GCTATCCTGC CTCTTGGGAC GGGCAATGAC 
CTAGCAAGAT GCCTGCGGTG GGGTGGAGGT TATGAAGGTG AAAATCTTAT GAAGATCCTA 
AAAGACATTG AAAGCAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCACACCC 
AACGACAAAG ACGAGAAAGG AGACCCTGTG CCTTACAGCA TCATCAACAA CTACTTTTCC 
ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCACATCA TGAGAGAAAA GCACCCGGAG 
AAATTCAACA GCCGAATGAA GAACAAATTT TGGTACTTTG AGTTTGGCAC ATCAGAAACC 
TTCTCAGCCA CCTGCAAGAA ACTGCATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG 
ATAGATTTGA TAAACATCTC TCTGGAAGGA ATTGCAATTT TGAATATACC AAGCATGCAT 
GGAGGATCTA ATCTTTGGGG AGAATCTAAA AAGAAAAGAA GTCATCGACG GATAGAGAAA 
AAAGGCTCTG ACAAAAGACC CACTCTCACA GATGCAAAAG AACTGAAGTT TGCAAGTCAA 
GATCTGAGTG ACCAGCTACT GGAGGTTGTT GGCTTGGAAG GAGCAATGGA AATGGGCCAA 
ATATACACGG GGCTGAAAAG TGCTGGCCGG CGGCTGGCTC AGTGCTCCTC TGTGGTTATC 
AGGACAAGCA AGTCACTGCC AATGCAGATT GATGGAGAGC CATGGATGCA GACCCCATGC 
ACAATAAAAA TTACACACAA GAACCAGGCC CCAATGCTGA TGGGACCTCC ACCTAAAACT 
GGATTATTCT GCTCCCTCAT CAAAAGAACA AGAAACCGAA GCAAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449819.1
CDS	410..2800
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	XM_017594330.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594330.1

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGACC ATAGATTTTG AAGGATTCAA GCTGTTCATG 
AAGACCTTCC TGGAAGCTGA GCTCCCTGAC GATTTCACTG CCCACCTTTT CATGTCATTC 
AGCAACAAAT TCCCCCATTC CAGCCCCAAC GTAAAGAGCA AGCCAGCTCT GCTTTCAGGT 
GGTTTGAGGA TGAATAAGGG TGCCATCACC CCTCCACGTT CTTCTCCAGC AAATACGTGC 
TCCCCGGAAG TGATCCACCT CAAGGATATT GTCTGTTACC TGTCTCTTCT GGAAAGAGGA 
AGACCTGAGG ACAAGCTTGA GTTTATGTTT CGCCTGTATG ACACCGATGG GAATGGCTTC 
CTGGACAGCT CGGAATTAGA AAATATCATC GGTCAGATGA TGCATGTTGC CGAGTACCTG 
GAATGGGATG TTACTGAACT TAATCCGATC CTTCATGAAA TGATGGAAGA AATCGACTAT 
GACCGTGACG GCACTGTGTC TCTGGAAGAG TGGATTCAAG GAGGAATGAC GACTATCCCA 
CTTCTAGTCT TGCTGGGCTT GGAAAACAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA 
CTCAAGCACT TTAACAAGCC CGCCTACTGC AATCTCTGCC TGAACATGCT GATTGGCGTG 
GGGAAGCAGG GCCTCTGCTG CTCCTTCTGC AAGTACACCG TCCATGAGCG CTGCGTGGCC 
AGAGCCCCGC CCTCCTGCAT CAAGACCTAT GTGAAGTCCA AGAAGAACAC TGACGTCATG 
CACCATTATT GGGTCGAAGG CAATTGCCCA ACCAAGTGTG ATAAGTGCCA CAAGACAGTG 
AAATGTTACC AGGGCCTGAC GGGGCTGCAC TGTGTTTGGT GTCAGACCAC GCTGCACAAT 
AAATGTGCTT CTCATCTGAA GCCTGAATGT GACTGTGGAC CCTTGAAGGA CCATATTTTG 
CCACCCACCA CAATCTGTCC AGTTGTATTG CAGACTATGC CCACTGCCGG AACTTCAGTT 
CCTGAGGAAA GACAGTCAAC AGCTAAAAAG GAAAAGGGCA GCTCCCAGCA GCCAAATAAA 
GTGACAGACA AGAACAAAAT GCAAAGAGCT AACTCTGTCA CTATGGATGG ACAAGGTTTG 
CAGATCACTC CTATTCCTGG CACACATCCT CTTCTAGTTT TTGTCAACCC TAAAAGTGGT 
GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAGTATC TTCTAAATCC TCGTCAGGTT 
TACAGTCTTT CTGGAAATGG ACCAATGCCA GGGTTACATT TTTTTCGCGA TGTCCCTGAC 
TTCAGAGTGT TAGCATGCGG TGGAGATGGG ACTGTGGGCT GGATTTTGGA CTGCATAGAA 
AAGGCCAATG TAGTCAAGCA CCCTCCAGTT GCTATCCTGC CTCTTGGGAC GGGCAATGAC 
CTAGCAAGAT GCCTGCGGTG GGGTGGAGGT TATGAAGGTG AAAATCTTAT GAAGATCCTA 
AAAGACATTG AAAGCAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCACACCC 
AACGACAAAG ACGAGAAAGG AGACCCTGTG CCTTACAGCA TCATCAACAA CTACTTTTCC 
ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCACATCA TGAGAGAAAA GCACCCGGAG 
AAATTCAACA GCCGAATGAA GAACAAATTT TGGTACTTTG AGTTTGGCAC ATCAGAAACC 
TTCTCAGCCA CCTGCAAGAA ACTGCATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG 
ATAGATTTGA TAAACATCTC TCTGGAAGGA ATTGCAATTT TGAATATACC AAGCATGCAT 
GGAGGATCTA ATCTTTGGGG AGAATCTAAA AAGAAAAGAA GTCATCGACG GATAGAGAAA 
AAAGGCTCTG ACAAAAGACC CACTCTCACA GATGCAAAAG AACTGAAGTT TGCAAGTCAA 
GATCTGAGTG ACCAGCTACT GGAGGTTGTT GGCTTGGAAG GAGCAATGGA AATGGGCCAA 
ATATACACGG GGCTGAAAAG TGCTGGCCGG CGGCTGGCTC AGTGCTCCTC TGTGGTTATC 
AGGACAAGCA AGTCACTGCC AATGCAGATT GATGGAGAGC CATGGATGCA GACCCCATGC 
ACAATAAAAA TTACACACAA GAACCAGGCC CCAATGCTGA TGGGACCTCC ACCTAAAACT 
GGATTATTCT GCTCCCTCAT CAAAAGAACA AGAAACCGAA GCAAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkb ( XM_017594329.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449818.1 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa61733
Clone ID Related Accession (Same CDS sequence)	XM_017594330.1 , XM_017594329.1
Accession Version	XM_017594329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGACC ATAGATTTTG AAGGATTCAA GCTGTTCATG 
AAGACCTTCC TGGAAGCTGA GCTCCCTGAC GATTTCACTG CCCACCTTTT CATGTCATTC 
AGCAACAAAT TCCCCCATTC CAGCCCCAAC GTAAAGAGCA AGCCAGCTCT GCTTTCAGGT 
GGTTTGAGGA TGAATAAGGG TGCCATCACC CCTCCACGTT CTTCTCCAGC AAATACGTGC 
TCCCCGGAAG TGATCCACCT CAAGGATATT GTCTGTTACC TGTCTCTTCT GGAAAGAGGA 
AGACCTGAGG ACAAGCTTGA GTTTATGTTT CGCCTGTATG ACACCGATGG GAATGGCTTC 
CTGGACAGCT CGGAATTAGA AAATATCATC GGTCAGATGA TGCATGTTGC CGAGTACCTG 
GAATGGGATG TTACTGAACT TAATCCGATC CTTCATGAAA TGATGGAAGA AATCGACTAT 
GACCGTGACG GCACTGTGTC TCTGGAAGAG TGGATTCAAG GAGGAATGAC GACTATCCCA 
CTTCTAGTCT TGCTGGGCTT GGAAAACAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA 
CTCAAGCACT TTAACAAGCC CGCCTACTGC AATCTCTGCC TGAACATGCT GATTGGCGTG 
GGGAAGCAGG GCCTCTGCTG CTCCTTCTGC AAGTACACCG TCCATGAGCG CTGCGTGGCC 
AGAGCCCCGC CCTCCTGCAT CAAGACCTAT GTGAAGTCCA AGAAGAACAC TGACGTCATG 
CACCATTATT GGGTCGAAGG CAATTGCCCA ACCAAGTGTG ATAAGTGCCA CAAGACAGTG 
AAATGTTACC AGGGCCTGAC GGGGCTGCAC TGTGTTTGGT GTCAGACCAC GCTGCACAAT 
AAATGTGCTT CTCATCTGAA GCCTGAATGT GACTGTGGAC CCTTGAAGGA CCATATTTTG 
CCACCCACCA CAATCTGTCC AGTTGTATTG CAGACTATGC CCACTGCCGG AACTTCAGTT 
CCTGAGGAAA GACAGTCAAC AGCTAAAAAG GAAAAGGGCA GCTCCCAGCA GCCAAATAAA 
GTGACAGACA AGAACAAAAT GCAAAGAGCT AACTCTGTCA CTATGGATGG ACAAGGTTTG 
CAGATCACTC CTATTCCTGG CACACATCCT CTTCTAGTTT TTGTCAACCC TAAAAGTGGT 
GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAGTATC TTCTAAATCC TCGTCAGGTT 
TACAGTCTTT CTGGAAATGG ACCAATGCCA GGGTTACATT TTTTTCGCGA TGTCCCTGAC 
TTCAGAGTGT TAGCATGCGG TGGAGATGGG ACTGTGGGCT GGATTTTGGA CTGCATAGAA 
AAGGCCAATG TAGTCAAGCA CCCTCCAGTT GCTATCCTGC CTCTTGGGAC GGGCAATGAC 
CTAGCAAGAT GCCTGCGGTG GGGTGGAGGT TATGAAGGTG AAAATCTTAT GAAGATCCTA 
AAAGACATTG AAAGCAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCACACCC 
AACGACAAAG ACGAGAAAGG AGACCCTGTG CCTTACAGCA TCATCAACAA CTACTTTTCC 
ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCACATCA TGAGAGAAAA GCACCCGGAG 
AAATTCAACA GCCGAATGAA GAACAAATTT TGGTACTTTG AGTTTGGCAC ATCAGAAACC 
TTCTCAGCCA CCTGCAAGAA ACTGCATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG 
ATAGATTTGA TAAACATCTC TCTGGAAGGA ATTGCAATTT TGAATATACC AAGCATGCAT 
GGAGGATCTA ATCTTTGGGG AGAATCTAAA AAGAAAAGAA GTCATCGACG GATAGAGAAA 
AAAGGCTCTG ACAAAAGACC CACTCTCACA GATGCAAAAG AACTGAAGTT TGCAAGTCAA 
GATCTGAGTG ACCAGCTACT GGAGGTTGTT GGCTTGGAAG GAGCAATGGA AATGGGCCAA 
ATATACACGG GGCTGAAAAG TGCTGGCCGG CGGCTGGCTC AGTGCTCCTC TGTGGTTATC 
AGGACAAGCA AGTCACTGCC AATGCAGATT GATGGAGAGC CATGGATGCA GACCCCATGC 
ACAATAAAAA TTACACACAA GAACCAGGCC CCAATGCTGA TGGGACCTCC ACCTAAAACT 
GGATTATTCT GCTCCCTCAT CAAAAGAACA AGAAACCGAA GCAAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449818.1
CDS	260..2650
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	XM_017594329.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594329.1

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGACC ATAGATTTTG AAGGATTCAA GCTGTTCATG 
AAGACCTTCC TGGAAGCTGA GCTCCCTGAC GATTTCACTG CCCACCTTTT CATGTCATTC 
AGCAACAAAT TCCCCCATTC CAGCCCCAAC GTAAAGAGCA AGCCAGCTCT GCTTTCAGGT 
GGTTTGAGGA TGAATAAGGG TGCCATCACC CCTCCACGTT CTTCTCCAGC AAATACGTGC 
TCCCCGGAAG TGATCCACCT CAAGGATATT GTCTGTTACC TGTCTCTTCT GGAAAGAGGA 
AGACCTGAGG ACAAGCTTGA GTTTATGTTT CGCCTGTATG ACACCGATGG GAATGGCTTC 
CTGGACAGCT CGGAATTAGA AAATATCATC GGTCAGATGA TGCATGTTGC CGAGTACCTG 
GAATGGGATG TTACTGAACT TAATCCGATC CTTCATGAAA TGATGGAAGA AATCGACTAT 
GACCGTGACG GCACTGTGTC TCTGGAAGAG TGGATTCAAG GAGGAATGAC GACTATCCCA 
CTTCTAGTCT TGCTGGGCTT GGAAAACAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA 
CTCAAGCACT TTAACAAGCC CGCCTACTGC AATCTCTGCC TGAACATGCT GATTGGCGTG 
GGGAAGCAGG GCCTCTGCTG CTCCTTCTGC AAGTACACCG TCCATGAGCG CTGCGTGGCC 
AGAGCCCCGC CCTCCTGCAT CAAGACCTAT GTGAAGTCCA AGAAGAACAC TGACGTCATG 
CACCATTATT GGGTCGAAGG CAATTGCCCA ACCAAGTGTG ATAAGTGCCA CAAGACAGTG 
AAATGTTACC AGGGCCTGAC GGGGCTGCAC TGTGTTTGGT GTCAGACCAC GCTGCACAAT 
AAATGTGCTT CTCATCTGAA GCCTGAATGT GACTGTGGAC CCTTGAAGGA CCATATTTTG 
CCACCCACCA CAATCTGTCC AGTTGTATTG CAGACTATGC CCACTGCCGG AACTTCAGTT 
CCTGAGGAAA GACAGTCAAC AGCTAAAAAG GAAAAGGGCA GCTCCCAGCA GCCAAATAAA 
GTGACAGACA AGAACAAAAT GCAAAGAGCT AACTCTGTCA CTATGGATGG ACAAGGTTTG 
CAGATCACTC CTATTCCTGG CACACATCCT CTTCTAGTTT TTGTCAACCC TAAAAGTGGT 
GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAGTATC TTCTAAATCC TCGTCAGGTT 
TACAGTCTTT CTGGAAATGG ACCAATGCCA GGGTTACATT TTTTTCGCGA TGTCCCTGAC 
TTCAGAGTGT TAGCATGCGG TGGAGATGGG ACTGTGGGCT GGATTTTGGA CTGCATAGAA 
AAGGCCAATG TAGTCAAGCA CCCTCCAGTT GCTATCCTGC CTCTTGGGAC GGGCAATGAC 
CTAGCAAGAT GCCTGCGGTG GGGTGGAGGT TATGAAGGTG AAAATCTTAT GAAGATCCTA 
AAAGACATTG AAAGCAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCACACCC 
AACGACAAAG ACGAGAAAGG AGACCCTGTG CCTTACAGCA TCATCAACAA CTACTTTTCC 
ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCACATCA TGAGAGAAAA GCACCCGGAG 
AAATTCAACA GCCGAATGAA GAACAAATTT TGGTACTTTG AGTTTGGCAC ATCAGAAACC 
TTCTCAGCCA CCTGCAAGAA ACTGCATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG 
ATAGATTTGA TAAACATCTC TCTGGAAGGA ATTGCAATTT TGAATATACC AAGCATGCAT 
GGAGGATCTA ATCTTTGGGG AGAATCTAAA AAGAAAAGAA GTCATCGACG GATAGAGAAA 
AAAGGCTCTG ACAAAAGACC CACTCTCACA GATGCAAAAG AACTGAAGTT TGCAAGTCAA 
GATCTGAGTG ACCAGCTACT GGAGGTTGTT GGCTTGGAAG GAGCAATGGA AATGGGCCAA 
ATATACACGG GGCTGAAAAG TGCTGGCCGG CGGCTGGCTC AGTGCTCCTC TGTGGTTATC 
AGGACAAGCA AGTCACTGCC AATGCAGATT GATGGAGAGC CATGGATGCA GACCCCATGC 
ACAATAAAAA TTACACACAA GAACCAGGCC CCAATGCTGA TGGGACCTCC ACCTAAAACT 
GGATTATTCT GCTCCCTCAT CAAAAGAACA AGAAACCGAA GCAAGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkb ( XM_017594331.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449820.1 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa61734
Clone ID Related Accession (Same CDS sequence)	XM_017594331.1
Accession Version	XM_017594331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGACC ATAGATTTTG AAGGATTCAA GCTGTTCATG 
AAGACCTTCC TGGAAGCTGA GCTCCCTGAC GATTTCACTG CCCACCTTTT CATGTCATTC 
AGCAACAAAT TCCCCCATTC CAGCCCCAAC GTAAAGAGCA AGCCAGCTCT GCTTTCAGGT 
GGTTTGAGGA TGAATAAGGG TGCCATCACC CCTCCACGTT CTTCTCCAGC AAATACGTGC 
TCCCCGGAAG TGATCCACCT CAAGGATATT GTCTGTTACC TGTCTCTTCT GGAAAGAGGA 
AGACCTGAGG ACAAGCTTGA GTTTATGTTT CGCCTGTATG ACACCGATGG GAATGGCTTC 
CTGGACAGCT CGGAATTAGA AAATATCATC GGTCAGATGA TGCATGTTGC CGAGTACCTG 
GAATGGGATG TTACTGAACT TAATCCGATC CTTCATGAAA TGATGGAAGA AATCGACTAT 
GACCGTGACG GCACTGTGTC TCTGGAAGAG TGGATTCAAG GAGGAATGAC GACTATCCCA 
CTTCTAGTCT TGCTGGGCTT GGAAAACAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA 
CTCAAGCACT TTAACAAGCC CGCCTACTGC AATCTCTGCC TGAACATGCT GATTGGCGTG 
GGGAAGCAGG GCCTCTGCTG CTCCTTCTGC AAGTACACCG TCCATGAGCG CTGCGTGGCC 
AGAGCCCCGC CCTCCTGCAT CAAGACCTAT GTGAAGTCCA AGAAGAACAC TGACGTCATG 
CACCATTATT GGGTCGAAGG CAATTGCCCA ACCAAGTGTG ATAAGTGCCA CAAGACAGTG 
AAATGTTACC AGGGCCTGAC GGGGCTGCAC TGTGTTTGGT GTCAGACCAC GCTGCACAAT 
AAATGTGCTT CTCATCTGAA GCCTGAATGT GACTGTGGAC CCTTGAAGGA CCATATTTTG 
CCACCCACCA CAATCTGTCC AGTTGTATTG ACTATGCCCA CTGCCGGAAC TTCAGTTCCT 
GAGGAAAGAC AGTCAACAGC TAAAAAGGAA AAGGGCAGCT CCCAGCAGCC AAATAAAGTG 
ACAGACAAGA ACAAAATGCA AAGAGCTAAC TCTGTCACTA TGGATGGACA AGGTTTGCAG 
ATCACTCCTA TTCCTGGCAC ACATCCTCTT CTAGTTTTTG TCAACCCTAA AAGTGGTGGG 
AAGCAAGGAG AACGAATTTA CAGAAAATTC CAGTATCTTC TAAATCCTCG TCAGGTTTAC 
AGTCTTTCTG GAAATGGACC AATGCCAGGG TTACATTTTT TTCGCGATGT CCCTGACTTC 
AGAGTGTTAG CATGCGGTGG AGATGGGACT GTGGGCTGGA TTTTGGACTG CATAGAAAAG 
GCCAATGTAG TCAAGCACCC TCCAGTTGCT ATCCTGCCTC TTGGGACGGG CAATGACCTA 
GCAAGATGCC TGCGGTGGGG TGGAGGTTAT GAAGGTGAAA ATCTTATGAA GATCCTAAAA 
GACATTGAAA GCAGCACAGA AATCATGTTG GACAGGTGGA AGTTTGAAGT CACACCCAAC 
GACAAAGACG AGAAAGGAGA CCCTGTGCCT TACAGCATCA TCAACAACTA CTTTTCCATT 
GGCGTGGATG CTTCCATTGC ACACAGATTC CACATCATGA GAGAAAAGCA CCCGGAGAAA 
TTCAACAGCC GAATGAAGAA CAAATTTTGG TACTTTGAGT TTGGCACATC AGAAACCTTC 
TCAGCCACCT GCAAGAAACT GCATGAATCT GTAGAAATAG AATGTGATGG AGTACAGATA 
GATTTGATAA ACATCTCTCT GGAAGGAATT GCAATTTTGA ATATACCAAG CATGCATGGA 
GGATCTAATC TTTGGGGAGA ATCTAAAAAG AAAAGAAGTC ATCGACGGAT AGAGAAAAAA 
GGCTCTGACA AAAGACCCAC TCTCACAGAT GCAAAAGAAC TGAAGTTTGC AAGTCAAGAT 
CTGAGTGACC AGCTACTGGA GGTTGTTGGC TTGGAAGGAG CAATGGAAAT GGGCCAAATA 
TACACGGGGC TGAAAAGTGC TGGCCGGCGG CTGGCTCAGT GCTCCTCTGT GGTTATCAGG 
ACAAGCAAGT CACTGCCAAT GCAGATTGAT GGAGAGCCAT GGATGCAGAC CCCATGCACA 
ATAAAAATTA CACACAAGAA CCAGGCCCCA ATGCTGATGG GACCTCCACC TAAAACTGGA 
TTATTCTGCT CCCTCATCAA AAGAACAAGA AACCGAAGCA AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449820.1
CDS	260..2647
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPTAGTSVPEERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	XM_017594331.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594331.1

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGACC ATAGATTTTG AAGGATTCAA GCTGTTCATG 
AAGACCTTCC TGGAAGCTGA GCTCCCTGAC GATTTCACTG CCCACCTTTT CATGTCATTC 
AGCAACAAAT TCCCCCATTC CAGCCCCAAC GTAAAGAGCA AGCCAGCTCT GCTTTCAGGT 
GGTTTGAGGA TGAATAAGGG TGCCATCACC CCTCCACGTT CTTCTCCAGC AAATACGTGC 
TCCCCGGAAG TGATCCACCT CAAGGATATT GTCTGTTACC TGTCTCTTCT GGAAAGAGGA 
AGACCTGAGG ACAAGCTTGA GTTTATGTTT CGCCTGTATG ACACCGATGG GAATGGCTTC 
CTGGACAGCT CGGAATTAGA AAATATCATC GGTCAGATGA TGCATGTTGC CGAGTACCTG 
GAATGGGATG TTACTGAACT TAATCCGATC CTTCATGAAA TGATGGAAGA AATCGACTAT 
GACCGTGACG GCACTGTGTC TCTGGAAGAG TGGATTCAAG GAGGAATGAC GACTATCCCA 
CTTCTAGTCT TGCTGGGCTT GGAAAACAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA 
CTCAAGCACT TTAACAAGCC CGCCTACTGC AATCTCTGCC TGAACATGCT GATTGGCGTG 
GGGAAGCAGG GCCTCTGCTG CTCCTTCTGC AAGTACACCG TCCATGAGCG CTGCGTGGCC 
AGAGCCCCGC CCTCCTGCAT CAAGACCTAT GTGAAGTCCA AGAAGAACAC TGACGTCATG 
CACCATTATT GGGTCGAAGG CAATTGCCCA ACCAAGTGTG ATAAGTGCCA CAAGACAGTG 
AAATGTTACC AGGGCCTGAC GGGGCTGCAC TGTGTTTGGT GTCAGACCAC GCTGCACAAT 
AAATGTGCTT CTCATCTGAA GCCTGAATGT GACTGTGGAC CCTTGAAGGA CCATATTTTG 
CCACCCACCA CAATCTGTCC AGTTGTATTG ACTATGCCCA CTGCCGGAAC TTCAGTTCCT 
GAGGAAAGAC AGTCAACAGC TAAAAAGGAA AAGGGCAGCT CCCAGCAGCC AAATAAAGTG 
ACAGACAAGA ACAAAATGCA AAGAGCTAAC TCTGTCACTA TGGATGGACA AGGTTTGCAG 
ATCACTCCTA TTCCTGGCAC ACATCCTCTT CTAGTTTTTG TCAACCCTAA AAGTGGTGGG 
AAGCAAGGAG AACGAATTTA CAGAAAATTC CAGTATCTTC TAAATCCTCG TCAGGTTTAC 
AGTCTTTCTG GAAATGGACC AATGCCAGGG TTACATTTTT TTCGCGATGT CCCTGACTTC 
AGAGTGTTAG CATGCGGTGG AGATGGGACT GTGGGCTGGA TTTTGGACTG CATAGAAAAG 
GCCAATGTAG TCAAGCACCC TCCAGTTGCT ATCCTGCCTC TTGGGACGGG CAATGACCTA 
GCAAGATGCC TGCGGTGGGG TGGAGGTTAT GAAGGTGAAA ATCTTATGAA GATCCTAAAA 
GACATTGAAA GCAGCACAGA AATCATGTTG GACAGGTGGA AGTTTGAAGT CACACCCAAC 
GACAAAGACG AGAAAGGAGA CCCTGTGCCT TACAGCATCA TCAACAACTA CTTTTCCATT 
GGCGTGGATG CTTCCATTGC ACACAGATTC CACATCATGA GAGAAAAGCA CCCGGAGAAA 
TTCAACAGCC GAATGAAGAA CAAATTTTGG TACTTTGAGT TTGGCACATC AGAAACCTTC 
TCAGCCACCT GCAAGAAACT GCATGAATCT GTAGAAATAG AATGTGATGG AGTACAGATA 
GATTTGATAA ACATCTCTCT GGAAGGAATT GCAATTTTGA ATATACCAAG CATGCATGGA 
GGATCTAATC TTTGGGGAGA ATCTAAAAAG AAAAGAAGTC ATCGACGGAT AGAGAAAAAA 
GGCTCTGACA AAAGACCCAC TCTCACAGAT GCAAAAGAAC TGAAGTTTGC AAGTCAAGAT 
CTGAGTGACC AGCTACTGGA GGTTGTTGGC TTGGAAGGAG CAATGGAAAT GGGCCAAATA 
TACACGGGGC TGAAAAGTGC TGGCCGGCGG CTGGCTCAGT GCTCCTCTGT GGTTATCAGG 
ACAAGCAAGT CACTGCCAAT GCAGATTGAT GGAGAGCCAT GGATGCAGAC CCCATGCACA 
ATAAAAATTA CACACAAGAA CCAGGCCCCA ATGCTGATGG GACCTCCACC TAAAACTGGA 
TTATTCTGCT CCCTCATCAA AAGAACAAGA AACCGAAGCA AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkb ( XM_017594332.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449821.1 Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa61735
Clone ID Related Accession (Same CDS sequence)	XM_017594332.1
Accession Version	XM_017594332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTCCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGCGTGGC CAGAGCCCCG CCCTCCTGCA TCAAGACCTA TGTGAAGTCC 
AAGAAGAACA CTGACGTCAT GCACCATTAT TGGGTCGAAG GCAATTGCCC AACCAAGTGT 
GATAAGTGCC ACAAGACAGT GAAATGTTAC CAGGGCCTGA CGGGGCTGCA CTGTGTTTGG 
TGTCAGACCA CGCTGCACAA TAAATGTGCT TCTCATCTGA AGCCTGAATG TGACTGTGGA 
CCCTTGAAGG ACCATATTTT GCCACCCACC ACAATCTGTC CAGTTGTATT GGAAAGACAG 
TCAACAGCTA AAAAGGAAAA GGGCAGCTCC CAGCAGCCAA ATAAAGTGAC AGACAAGAAC 
AAAATGCAAA GAGCTAACTC TGTCACTATG GATGGACAAG GTTTGCAGAT CACTCCTATT 
CCTGGCACAC ATCCTCTTCT AGTTTTTGTC AACCCTAAAA GTGGTGGGAA GCAAGGAGAA 
CGAATTTACA GAAAATTCCA GTATCTTCTA AATCCTCGTC AGGTTTACAG TCTTTCTGGA 
AATGGACCAA TGCCAGGGTT ACATTTTTTT CGCGATGTCC CTGACTTCAG AGTGTTAGCA 
TGCGGTGGAG ATGGGACTGT GGGCTGGATT TTGGACTGCA TAGAAAAGGC CAATGTAGTC 
AAGCACCCTC CAGTTGCTAT CCTGCCTCTT GGGACGGGCA ATGACCTAGC AAGATGCCTG 
CGGTGGGGTG GAGGTTATGA AGGTGAAAAT CTTATGAAGA TCCTAAAAGA CATTGAAAGC 
AGCACAGAAA TCATGTTGGA CAGGTGGAAG TTTGAAGTCA CACCCAACGA CAAAGACGAG 
AAAGGAGACC CTGTGCCTTA CAGCATCATC AACAACTACT TTTCCATTGG CGTGGATGCT 
TCCATTGCAC ACAGATTCCA CATCATGAGA GAAAAGCACC CGGAGAAATT CAACAGCCGA 
ATGAAGAACA AATTTTGGTA CTTTGAGTTT GGCACATCAG AAACCTTCTC AGCCACCTGC 
AAGAAACTGC ATGAATCTGT AGAAATAGAA TGTGATGGAG TACAGATAGA TTTGATAAAC 
ATCTCTCTGG AAGGAATTGC AATTTTGAAT ATACCAAGCA TGCATGGAGG ATCTAATCTT 
TGGGGAGAAT CTAAAAAGAA AAGAAGTCAT CGACGGATAG AGAAAAAAGG CTCTGACAAA 
AGACCCACTC TCACAGATGC AAAAGAACTG AAGTTTGCAA GTCAAGATCT GAGTGACCAG 
CTACTGGAGG TTGTTGGCTT GGAAGGAGCA ATGGAAATGG GCCAAATATA CACGGGGCTG 
AAAAGTGCTG GCCGGCGGCT GGCTCAGTGC TCCTCTGTGG TTATCAGGAC AAGCAAGTCA 
CTGCCAATGC AGATTGATGG AGAGCCATGG ATGCAGACCC CATGCACAAT AAAAATTACA 
CACAAGAACC AGGCCCCAAT GCTGATGGGA CCTCCACCTA AAACTGGATT ATTCTGCTCC 
CTCATCAAAA GAACAAGAAA CCGAAGCAAG GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449821.1
CDS	260..2635
Translation	MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKPALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLERQSTAKKEKGSSQQPNKVTDKNKMQRANSVTMDGQGLQITPIPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRNRSKE

Target ORF information:

RefSeq Version	XM_017594332.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, beta (Dgkb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594332.1

ORF Insert Sequence:

ATGACAAACC AGGAAAAATG GGCCCATCTC AGCCCCTCAG AATTTTCCCA ACTTCAGAAA 
TATGCTGAGT ATTCTACAAA GAAATTAAAG GATGTTCTTG AAGAATTCCA TGGTAATGGT 
GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGATATTC TTAACCAAAC CATAGATTTT 
GAAGGATTCA AGCTGTTCAT GAAGACCTTC CTGGAAGCTG AGCTCCCTGA CGATTTCACT 
GCCCACCTTT TCATGTCATT CAGCAACAAA TTCCCCCATT CCAGCCCCAA CGTAAAGAGC 
AAGCCAGCTC TGCTTTCAGG TGGTTTGAGG ATGAATAAGG GTGCCATCAC CCCTCCACGT 
TCTTCTCCAG CAAATACGTG CTCCCCGGAA GTGATCCACC TCAAGGATAT TGTCTGTTAC 
CTGTCTCTTC TGGAAAGAGG AAGACCTGAG GACAAGCTTG AGTTTATGTT TCGCCTGTAT 
GACACCGATG GGAATGGCTT CCTGGACAGC TCGGAATTAG AAAATATCAT CGGTCAGATG 
ATGCATGTTG CCGAGTACCT GGAATGGGAT GTTACTGAAC TTAATCCGAT CCTTCATGAA 
ATGATGGAAG AAATCGACTA TGACCGTGAC GGCACTGTGT CTCTGGAAGA GTGGATTCAA 
GGAGGAATGA CGACTATCCC ACTTCTAGTC TTGCTGGGCT TGGAAAACAA TGTGAAGGAT 
GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAGC CCGCCTACTG CAATCTCTGC 
CTGAACATGC TGATTGGCGT GGGGAAGCAG GGCCTCTGCT GCTCCTTCTG CAAGTACACC 
GTCCATGAGC GCTGCGTGGC CAGAGCCCCG CCCTCCTGCA TCAAGACCTA TGTGAAGTCC 
AAGAAGAACA CTGACGTCAT GCACCATTAT TGGGTCGAAG GCAATTGCCC AACCAAGTGT 
GATAAGTGCC ACAAGACAGT GAAATGTTAC CAGGGCCTGA CGGGGCTGCA CTGTGTTTGG 
TGTCAGACCA CGCTGCACAA TAAATGTGCT TCTCATCTGA AGCCTGAATG TGACTGTGGA 
CCCTTGAAGG ACCATATTTT GCCACCCACC ACAATCTGTC CAGTTGTATT GGAAAGACAG 
TCAACAGCTA AAAAGGAAAA GGGCAGCTCC CAGCAGCCAA ATAAAGTGAC AGACAAGAAC 
AAAATGCAAA GAGCTAACTC TGTCACTATG GATGGACAAG GTTTGCAGAT CACTCCTATT 
CCTGGCACAC ATCCTCTTCT AGTTTTTGTC AACCCTAAAA GTGGTGGGAA GCAAGGAGAA 
CGAATTTACA GAAAATTCCA GTATCTTCTA AATCCTCGTC AGGTTTACAG TCTTTCTGGA 
AATGGACCAA TGCCAGGGTT ACATTTTTTT CGCGATGTCC CTGACTTCAG AGTGTTAGCA 
TGCGGTGGAG ATGGGACTGT GGGCTGGATT TTGGACTGCA TAGAAAAGGC CAATGTAGTC 
AAGCACCCTC CAGTTGCTAT CCTGCCTCTT GGGACGGGCA ATGACCTAGC AAGATGCCTG 
CGGTGGGGTG GAGGTTATGA AGGTGAAAAT CTTATGAAGA TCCTAAAAGA CATTGAAAGC 
AGCACAGAAA TCATGTTGGA CAGGTGGAAG TTTGAAGTCA CACCCAACGA CAAAGACGAG 
AAAGGAGACC CTGTGCCTTA CAGCATCATC AACAACTACT TTTCCATTGG CGTGGATGCT 
TCCATTGCAC ACAGATTCCA CATCATGAGA GAAAAGCACC CGGAGAAATT CAACAGCCGA 
ATGAAGAACA AATTTTGGTA CTTTGAGTTT GGCACATCAG AAACCTTCTC AGCCACCTGC 
AAGAAACTGC ATGAATCTGT AGAAATAGAA TGTGATGGAG TACAGATAGA TTTGATAAAC 
ATCTCTCTGG AAGGAATTGC AATTTTGAAT ATACCAAGCA TGCATGGAGG ATCTAATCTT 
TGGGGAGAAT CTAAAAAGAA AAGAAGTCAT CGACGGATAG AGAAAAAAGG CTCTGACAAA 
AGACCCACTC TCACAGATGC AAAAGAACTG AAGTTTGCAA GTCAAGATCT GAGTGACCAG 
CTACTGGAGG TTGTTGGCTT GGAAGGAGCA ATGGAAATGG GCCAAATATA CACGGGGCTG 
AAAAGTGCTG GCCGGCGGCT GGCTCAGTGC TCCTCTGTGG TTATCAGGAC AAGCAAGTCA 
CTGCCAATGC AGATTGATGG AGAGCCATGG ATGCAGACCC CATGCACAAT AAAAATTACA 
CACAAGAACC AGGCCCCAAT GCTGATGGGA CCTCCACCTA AAACTGGATT ATTCTGCTCC 
CTCATCAAAA GAACAAGAAA CCGAAGCAAG GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes.
Brain structure & function219(2)719-49(2014 Mar)
Sekerkov? G,Watanabe M,Martina M,Mugnaini E

Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society61(6)462-76(2013 Jun)
Hozumi Y,Matsui H,Sakane F,Watanabe M,Goto K

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.
Biochemical and biophysical research communications422(4)758-63(2012 Jun)
Gellett AM,Kharel Y,Sunkara M,Morris AJ,Lynch KR

Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function.
PloS one5(7)e11602(2010 Jul)
Shirai Y,Kouzuki T,Kakefuda K,Moriguchi S,Oyagi A,Horie K,Morita SY,Shimazawa M,Fukunaga K,Takeda J,Saito N,Hara H

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Dgkb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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