DGKB cDNA ORF clone, Bos taurus(cattle)

The following DGKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa18412 NM_001192898.1
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa18412 XM_010804042.2
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa18412 XM_005205200.3
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa18412 XM_015468340.2
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa18413 XM_005205201.4
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa53548 XM_010804043.2
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa53549 XM_010804044.3
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa53550 XM_010804045.2
Latest version!
Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
OBa53549 XM_010804044.2 Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa18412 XM_015468340.1 Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa18413 XM_005205201.3 Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa18412
    Clone ID Related Accession (Same CDS sequence) NM_001192898.1 , XM_005205200.3 , XM_015468340.1 , XM_010804042.2 , XM_015468340.2
    Accession Version NM_001192898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000004.1. On Jul 17, 2010 this sequence version replaced XM_617327.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179827.1
    CDS38..2449
    Translation

    Target ORF information:

    RefSeq Version NM_001192898.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18412
    Clone ID Related Accession (Same CDS sequence) NM_001192898.1 , XM_005205200.3 , XM_015468340.1 , XM_010804042.2 , XM_015468340.2
    Accession Version XM_005205200.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205257.1
    CDS444..2855
    Translation

    Target ORF information:

    RefSeq Version XM_005205200.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205200.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18412
    Clone ID Related Accession (Same CDS sequence) NM_001192898.1 , XM_005205200.3 , XM_015468340.1 , XM_010804042.2 , XM_015468340.2
    Accession Version XM_015468340.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323826.1
    CDS542..2953
    Translation

    Target ORF information:

    RefSeq Version XM_015468340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18412
    Clone ID Related Accession (Same CDS sequence) NM_001192898.1 , XM_005205200.3 , XM_015468340.1 , XM_010804042.2 , XM_015468340.2
    Accession Version XM_010804042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802344.1
    CDS561..2972
    Translation

    Target ORF information:

    RefSeq Version XM_010804042.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18412
    Clone ID Related Accession (Same CDS sequence) NM_001192898.1 , XM_005205200.3 , XM_015468340.1 , XM_010804042.2 , XM_015468340.2
    Accession Version XM_015468340.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2412bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015468340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323826.1
    CDS557..2968
    Translation

    Target ORF information:

    RefSeq Version XM_015468340.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468340.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GCAGACTCTG
    CCCACTTCAG GAGTTTCAGC CCCTGAGGAA AGACAAGCGA CAGTGAAAAG GGAAAAGGGT
    AGTTCCCAGC AGTCAAACAA AGTGACCGAC AAGAACAAAA TGCAAAGAGC TAACTCTGTT
    ACTGTGGATG GACAAGGCCT GCAGATCACT CCAGTTCCTG GTACTCATCC ACTTTTAGTT
    TTTGTGAACC CCAAAAGTGG TGGGAAGCAA GGAGAACGAA TTTACAGAAA ATTCCAATAC
    CTACTAAATC CTCGTCAGGT TTACAGTCTT GCTGGAAATG GACCAATGCC AGGGTTAAAC
    TTTTTCCGTG ATGTTCCTGA TTTCAGAGTG TTAGCCTGTG GTGGAGATGG AACTGTGGGG
    TGGATTTTGG ACTGCATAGA AAAGGCTAAT GTAGTCAAGC ATCCTCCAGT GGCTATTCTG
    CCTCTCGGGA CCGGCAATGA TCTAGCAAGA TGCCTGCGAT GGGGAGGAGG TTATGAAGGT
    GAGAATTTGA TGAAAATCTT AAAAGACATT GAAAACAGCA CAGAAATCAT GTTGGACAGG
    TGGAAGTTTG AAGTCATACC TAATGACAAA GATGAGAAGG GAGACCCAGT GCCTTACAGT
    ATCATCAATA ATTATTTTTC CATTGGCGTG GATGCTTCCA TTGCACACAG ATTCCATATC
    ATGAGAGAGA AACACCCAGA AAAATTCAAT AGTAGAATGA AGAACAAATT TTGGTATTTT
    GAGTTTGGCA CATCTGAAAC TTTCTCAGCC ACCTGCAAGA AGCTACATGA ATCTGTAGAA
    ATAGAATGTG ATGGAGTACA GATTGACTTA ATAAACATCT CTCTGGAAGG AATTGCTATT
    TTGAATATAC CCAGCATGCA TGGAGGGTCC AATCTTTGGG GAGAGTCTAA GAAAAGACGA
    AGCCATCGCC GAATAGAGAA AAAAGGGTCT GACAAAAGGA CCACGCTCAT AGATGCCAAA
    GAGTTGAAGT TTGCAAGTCA AGATCTGAGT GATCAGCTGC TGGAGGTGGT TGGCTTGGAA
    GGAGCCATGG AGATGGGGCA AATATACACA GGACTGAAAA GTGCTGGGCG GCGGCTGGCT
    CAGTGCTCCT CTGTTGTCAT CAGGACCAGC AAGTCTCTGC CAATGCAGAT TGATGGGGAA
    CCATGGATGC AGACCCCATG CACTATAAAA ATTATACACA AGAACCAAGC CCCAATGCTG
    ATGGGTCCTC CTCCCAAAAC CGGGTTGTTC TGCTCCCTCG TCAAAAGAAC CAGAAACCGA
    AGCAAGGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18413
    Clone ID Related Accession (Same CDS sequence) XM_005205201.3 , XM_005205201.4
    Accession Version XM_005205201.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG CAGACTCTGC CCACTTCAGG AGTTTCAGCC
    CCTGAGGAAA GACAAGCGAC AGTGAAAAGG GAAAAGGGTA GTTCCCAGCA GTCAAACAAA
    GTGACCGACA AGAACAAAAT GCAAAGAGCT AACTCTGTTA CTGTGGATGG ACAAGGCCTG
    CAGATCACTC CAGTTCCTGG TACTCATCCA CTTTTAGTTT TTGTGAACCC CAAAAGTGGT
    GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAATACC TACTAAATCC TCGTCAGGTT
    TACAGTCTTG CTGGAAATGG ACCAATGCCA GGGTTAAACT TTTTCCGTGA TGTTCCTGAT
    TTCAGAGTGT TAGCCTGTGG TGGAGATGGA ACTGTGGGGT GGATTTTGGA CTGCATAGAA
    AAGGCTAATG TAGTCAAGCA TCCTCCAGTG GCTATTCTGC CTCTCGGGAC CGGCAATGAT
    CTAGCAAGAT GCCTGCGATG GGGAGGAGGT TATGAAGGTG AGAATTTGAT GAAAATCTTA
    AAAGACATTG AAAACAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCATACCT
    AATGACAAAG ATGAGAAGGG AGACCCAGTG CCTTACAGTA TCATCAATAA TTATTTTTCC
    ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCATATCA TGAGAGAGAA ACACCCAGAA
    AAATTCAATA GTAGAATGAA GAACAAATTT TGGTATTTTG AGTTTGGCAC ATCTGAAACT
    TTCTCAGCCA CCTGCAAGAA GCTACATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG
    ATTGACTTAA TAAACATCTC TCTGGAAGGA ATTGCTATTT TGAATATACC CAGCATGCAT
    GGAGGGTCCA ATCTTTGGGG AGAGTCTAAG AAAAGACGAA GCCATCGCCG AATAGAGAAA
    AAAGGGTCTG ACAAAAGGAC CACGCTCATA GATGCCAAAG AGTTGAAGTT TGCAAGTCAA
    GATCTGAGTG ATCAGCTGCT GGAGGTGGTT GGCTTGGAAG GAGCCATGGA GATGGGGCAA
    ATATACACAG GACTGAAAAG TGCTGGGCGG CGGCTGGCTC AGTGCTCCTC TGTTGTCATC
    AGGACCAGCA AGTCTCTGCC AATGCAGATT GATGGGGAAC CATGGATGCA GACCCCATGC
    ACTATAAAAA TTATACACAA GAACCAAGCC CCAATGCTGA TGGGTCCTCC TCCCAAAACC
    GGGTTGTTCT GCTCCCTCGT CAAAAGAACC AGAAACCGAA GCAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205258.1
    CDS542..2932
    Translation

    Target ORF information:

    RefSeq Version XM_005205201.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205201.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG CAGACTCTGC CCACTTCAGG AGTTTCAGCC
    CCTGAGGAAA GACAAGCGAC AGTGAAAAGG GAAAAGGGTA GTTCCCAGCA GTCAAACAAA
    GTGACCGACA AGAACAAAAT GCAAAGAGCT AACTCTGTTA CTGTGGATGG ACAAGGCCTG
    CAGATCACTC CAGTTCCTGG TACTCATCCA CTTTTAGTTT TTGTGAACCC CAAAAGTGGT
    GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAATACC TACTAAATCC TCGTCAGGTT
    TACAGTCTTG CTGGAAATGG ACCAATGCCA GGGTTAAACT TTTTCCGTGA TGTTCCTGAT
    TTCAGAGTGT TAGCCTGTGG TGGAGATGGA ACTGTGGGGT GGATTTTGGA CTGCATAGAA
    AAGGCTAATG TAGTCAAGCA TCCTCCAGTG GCTATTCTGC CTCTCGGGAC CGGCAATGAT
    CTAGCAAGAT GCCTGCGATG GGGAGGAGGT TATGAAGGTG AGAATTTGAT GAAAATCTTA
    AAAGACATTG AAAACAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCATACCT
    AATGACAAAG ATGAGAAGGG AGACCCAGTG CCTTACAGTA TCATCAATAA TTATTTTTCC
    ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCATATCA TGAGAGAGAA ACACCCAGAA
    AAATTCAATA GTAGAATGAA GAACAAATTT TGGTATTTTG AGTTTGGCAC ATCTGAAACT
    TTCTCAGCCA CCTGCAAGAA GCTACATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG
    ATTGACTTAA TAAACATCTC TCTGGAAGGA ATTGCTATTT TGAATATACC CAGCATGCAT
    GGAGGGTCCA ATCTTTGGGG AGAGTCTAAG AAAAGACGAA GCCATCGCCG AATAGAGAAA
    AAAGGGTCTG ACAAAAGGAC CACGCTCATA GATGCCAAAG AGTTGAAGTT TGCAAGTCAA
    GATCTGAGTG ATCAGCTGCT GGAGGTGGTT GGCTTGGAAG GAGCCATGGA GATGGGGCAA
    ATATACACAG GACTGAAAAG TGCTGGGCGG CGGCTGGCTC AGTGCTCCTC TGTTGTCATC
    AGGACCAGCA AGTCTCTGCC AATGCAGATT GATGGGGAAC CATGGATGCA GACCCCATGC
    ACTATAAAAA TTATACACAA GAACCAAGCC CCAATGCTGA TGGGTCCTCC TCCCAAAACC
    GGGTTGTTCT GCTCCCTCGT CAAAAGAACC AGAAACCGAA GCAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18413
    Clone ID Related Accession (Same CDS sequence) XM_005205201.3 , XM_005205201.4
    Accession Version XM_005205201.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2391bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205201.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG CAGACTCTGC CCACTTCAGG AGTTTCAGCC
    CCTGAGGAAA GACAAGCGAC AGTGAAAAGG GAAAAGGGTA GTTCCCAGCA GTCAAACAAA
    GTGACCGACA AGAACAAAAT GCAAAGAGCT AACTCTGTTA CTGTGGATGG ACAAGGCCTG
    CAGATCACTC CAGTTCCTGG TACTCATCCA CTTTTAGTTT TTGTGAACCC CAAAAGTGGT
    GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAATACC TACTAAATCC TCGTCAGGTT
    TACAGTCTTG CTGGAAATGG ACCAATGCCA GGGTTAAACT TTTTCCGTGA TGTTCCTGAT
    TTCAGAGTGT TAGCCTGTGG TGGAGATGGA ACTGTGGGGT GGATTTTGGA CTGCATAGAA
    AAGGCTAATG TAGTCAAGCA TCCTCCAGTG GCTATTCTGC CTCTCGGGAC CGGCAATGAT
    CTAGCAAGAT GCCTGCGATG GGGAGGAGGT TATGAAGGTG AGAATTTGAT GAAAATCTTA
    AAAGACATTG AAAACAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCATACCT
    AATGACAAAG ATGAGAAGGG AGACCCAGTG CCTTACAGTA TCATCAATAA TTATTTTTCC
    ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCATATCA TGAGAGAGAA ACACCCAGAA
    AAATTCAATA GTAGAATGAA GAACAAATTT TGGTATTTTG AGTTTGGCAC ATCTGAAACT
    TTCTCAGCCA CCTGCAAGAA GCTACATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG
    ATTGACTTAA TAAACATCTC TCTGGAAGGA ATTGCTATTT TGAATATACC CAGCATGCAT
    GGAGGGTCCA ATCTTTGGGG AGAGTCTAAG AAAAGACGAA GCCATCGCCG AATAGAGAAA
    AAAGGGTCTG ACAAAAGGAC CACGCTCATA GATGCCAAAG AGTTGAAGTT TGCAAGTCAA
    GATCTGAGTG ATCAGCTGCT GGAGGTGGTT GGCTTGGAAG GAGCCATGGA GATGGGGCAA
    ATATACACAG GACTGAAAAG TGCTGGGCGG CGGCTGGCTC AGTGCTCCTC TGTTGTCATC
    AGGACCAGCA AGTCTCTGCC AATGCAGATT GATGGGGAAC CATGGATGCA GACCCCATGC
    ACTATAAAAA TTATACACAA GAACCAAGCC CCAATGCTGA TGGGTCCTCC TCCCAAAACC
    GGGTTGTTCT GCTCCCTCGT CAAAAGAACC AGAAACCGAA GCAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205258.1
    CDS557..2947
    Translation

    Target ORF information:

    RefSeq Version XM_005205201.4
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205201.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG CAGACTCTGC CCACTTCAGG AGTTTCAGCC
    CCTGAGGAAA GACAAGCGAC AGTGAAAAGG GAAAAGGGTA GTTCCCAGCA GTCAAACAAA
    GTGACCGACA AGAACAAAAT GCAAAGAGCT AACTCTGTTA CTGTGGATGG ACAAGGCCTG
    CAGATCACTC CAGTTCCTGG TACTCATCCA CTTTTAGTTT TTGTGAACCC CAAAAGTGGT
    GGGAAGCAAG GAGAACGAAT TTACAGAAAA TTCCAATACC TACTAAATCC TCGTCAGGTT
    TACAGTCTTG CTGGAAATGG ACCAATGCCA GGGTTAAACT TTTTCCGTGA TGTTCCTGAT
    TTCAGAGTGT TAGCCTGTGG TGGAGATGGA ACTGTGGGGT GGATTTTGGA CTGCATAGAA
    AAGGCTAATG TAGTCAAGCA TCCTCCAGTG GCTATTCTGC CTCTCGGGAC CGGCAATGAT
    CTAGCAAGAT GCCTGCGATG GGGAGGAGGT TATGAAGGTG AGAATTTGAT GAAAATCTTA
    AAAGACATTG AAAACAGCAC AGAAATCATG TTGGACAGGT GGAAGTTTGA AGTCATACCT
    AATGACAAAG ATGAGAAGGG AGACCCAGTG CCTTACAGTA TCATCAATAA TTATTTTTCC
    ATTGGCGTGG ATGCTTCCAT TGCACACAGA TTCCATATCA TGAGAGAGAA ACACCCAGAA
    AAATTCAATA GTAGAATGAA GAACAAATTT TGGTATTTTG AGTTTGGCAC ATCTGAAACT
    TTCTCAGCCA CCTGCAAGAA GCTACATGAA TCTGTAGAAA TAGAATGTGA TGGAGTACAG
    ATTGACTTAA TAAACATCTC TCTGGAAGGA ATTGCTATTT TGAATATACC CAGCATGCAT
    GGAGGGTCCA ATCTTTGGGG AGAGTCTAAG AAAAGACGAA GCCATCGCCG AATAGAGAAA
    AAAGGGTCTG ACAAAAGGAC CACGCTCATA GATGCCAAAG AGTTGAAGTT TGCAAGTCAA
    GATCTGAGTG ATCAGCTGCT GGAGGTGGTT GGCTTGGAAG GAGCCATGGA GATGGGGCAA
    ATATACACAG GACTGAAAAG TGCTGGGCGG CGGCTGGCTC AGTGCTCCTC TGTTGTCATC
    AGGACCAGCA AGTCTCTGCC AATGCAGATT GATGGGGAAC CATGGATGCA GACCCCATGC
    ACTATAAAAA TTATACACAA GAACCAAGCC CCAATGCTGA TGGGTCCTCC TCCCAAAACC
    GGGTTGTTCT GCTCCCTCGT CAAAAGAACC AGAAACCGAA GCAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53548
    Clone ID Related Accession (Same CDS sequence) XM_010804043.2
    Accession Version XM_010804043.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GACTCTGCCC
    ACTTCAGGAG TTTCAGCCCC TGAGGAAAGA CAAGCGACAG TGAAAAGGGA AAAGGGTAGT
    TCCCAGCAGT CAAACAAAGT GACCGACAAG AACAAAATGC AAAGAGCTAA CTCTGTTACT
    GTGGATGGAC AAGGCCTGCA GATCACTCCA GTTCCTGGTA CTCATCCACT TTTAGTTTTT
    GTGAACCCCA AAAGTGGTGG GAAGCAAGGA GAACGAATTT ACAGAAAATT CCAATACCTA
    CTAAATCCTC GTCAGGTTTA CAGTCTTGCT GGAAATGGAC CAATGCCAGG GTTAAACTTT
    TTCCGTGATG TTCCTGATTT CAGAGTGTTA GCCTGTGGTG GAGATGGAAC TGTGGGGTGG
    ATTTTGGACT GCATAGAAAA GGCTAATGTA GTCAAGCATC CTCCAGTGGC TATTCTGCCT
    CTCGGGACCG GCAATGATCT AGCAAGATGC CTGCGATGGG GAGGAGGTTA TGAAGGTGAG
    AATTTGATGA AAATCTTAAA AGACATTGAA AACAGCACAG AAATCATGTT GGACAGGTGG
    AAGTTTGAAG TCATACCTAA TGACAAAGAT GAGAAGGGAG ACCCAGTGCC TTACAGTATC
    ATCAATAATT ATTTTTCCAT TGGCGTGGAT GCTTCCATTG CACACAGATT CCATATCATG
    AGAGAGAAAC ACCCAGAAAA ATTCAATAGT AGAATGAAGA ACAAATTTTG GTATTTTGAG
    TTTGGCACAT CTGAAACTTT CTCAGCCACC TGCAAGAAGC TACATGAATC TGTAGAAATA
    GAATGTGATG GAGTACAGAT TGACTTAATA AACATCTCTC TGGAAGGAAT TGCTATTTTG
    AATATACCCA GCATGCATGG AGGGTCCAAT CTTTGGGGAG AGTCTAAGAA AAGACGAAGC
    CATCGCCGAA TAGAGAAAAA AGGGTCTGAC AAAAGGACCA CGCTCATAGA TGCCAAAGAG
    TTGAAGTTTG CAAGTCAAGA TCTGAGTGAT CAGCTGCTGG AGGTGGTTGG CTTGGAAGGA
    GCCATGGAGA TGGGGCAAAT ATACACAGGA CTGAAAAGTG CTGGGCGGCG GCTGGCTCAG
    TGCTCCTCTG TTGTCATCAG GACCAGCAAG TCTCTGCCAA TGCAGATTGA TGGGGAACCA
    TGGATGCAGA CCCCATGCAC TATAAAAATT ATACACAAGA ACCAAGCCCC AATGCTGATG
    GGTCCTCCTC CCAAAACCGG GTTGTTCTGC TCCCTCGTCA AAAGAACCAG AAACCGAAGC
    AAGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802345.1
    CDS542..2950
    Translation

    Target ORF information:

    RefSeq Version XM_010804043.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GACTCTGCCC
    ACTTCAGGAG TTTCAGCCCC TGAGGAAAGA CAAGCGACAG TGAAAAGGGA AAAGGGTAGT
    TCCCAGCAGT CAAACAAAGT GACCGACAAG AACAAAATGC AAAGAGCTAA CTCTGTTACT
    GTGGATGGAC AAGGCCTGCA GATCACTCCA GTTCCTGGTA CTCATCCACT TTTAGTTTTT
    GTGAACCCCA AAAGTGGTGG GAAGCAAGGA GAACGAATTT ACAGAAAATT CCAATACCTA
    CTAAATCCTC GTCAGGTTTA CAGTCTTGCT GGAAATGGAC CAATGCCAGG GTTAAACTTT
    TTCCGTGATG TTCCTGATTT CAGAGTGTTA GCCTGTGGTG GAGATGGAAC TGTGGGGTGG
    ATTTTGGACT GCATAGAAAA GGCTAATGTA GTCAAGCATC CTCCAGTGGC TATTCTGCCT
    CTCGGGACCG GCAATGATCT AGCAAGATGC CTGCGATGGG GAGGAGGTTA TGAAGGTGAG
    AATTTGATGA AAATCTTAAA AGACATTGAA AACAGCACAG AAATCATGTT GGACAGGTGG
    AAGTTTGAAG TCATACCTAA TGACAAAGAT GAGAAGGGAG ACCCAGTGCC TTACAGTATC
    ATCAATAATT ATTTTTCCAT TGGCGTGGAT GCTTCCATTG CACACAGATT CCATATCATG
    AGAGAGAAAC ACCCAGAAAA ATTCAATAGT AGAATGAAGA ACAAATTTTG GTATTTTGAG
    TTTGGCACAT CTGAAACTTT CTCAGCCACC TGCAAGAAGC TACATGAATC TGTAGAAATA
    GAATGTGATG GAGTACAGAT TGACTTAATA AACATCTCTC TGGAAGGAAT TGCTATTTTG
    AATATACCCA GCATGCATGG AGGGTCCAAT CTTTGGGGAG AGTCTAAGAA AAGACGAAGC
    CATCGCCGAA TAGAGAAAAA AGGGTCTGAC AAAAGGACCA CGCTCATAGA TGCCAAAGAG
    TTGAAGTTTG CAAGTCAAGA TCTGAGTGAT CAGCTGCTGG AGGTGGTTGG CTTGGAAGGA
    GCCATGGAGA TGGGGCAAAT ATACACAGGA CTGAAAAGTG CTGGGCGGCG GCTGGCTCAG
    TGCTCCTCTG TTGTCATCAG GACCAGCAAG TCTCTGCCAA TGCAGATTGA TGGGGAACCA
    TGGATGCAGA CCCCATGCAC TATAAAAATT ATACACAAGA ACCAAGCCCC AATGCTGATG
    GGTCCTCCTC CCAAAACCGG GTTGTTCTGC TCCCTCGTCA AAAGAACCAG AAACCGAAGC
    AAGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53549
    Clone ID Related Accession (Same CDS sequence) XM_010804044.3 , XM_010804044.2
    Accession Version XM_010804044.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG ACTCTGCCCA CTTCAGGAGT TTCAGCCCCT
    GAGGAAAGAC AAGCGACAGT GAAAAGGGAA AAGGGTAGTT CCCAGCAGTC AAACAAAGTG
    ACCGACAAGA ACAAAATGCA AAGAGCTAAC TCTGTTACTG TGGATGGACA AGGCCTGCAG
    ATCACTCCAG TTCCTGGTAC TCATCCACTT TTAGTTTTTG TGAACCCCAA AAGTGGTGGG
    AAGCAAGGAG AACGAATTTA CAGAAAATTC CAATACCTAC TAAATCCTCG TCAGGTTTAC
    AGTCTTGCTG GAAATGGACC AATGCCAGGG TTAAACTTTT TCCGTGATGT TCCTGATTTC
    AGAGTGTTAG CCTGTGGTGG AGATGGAACT GTGGGGTGGA TTTTGGACTG CATAGAAAAG
    GCTAATGTAG TCAAGCATCC TCCAGTGGCT ATTCTGCCTC TCGGGACCGG CAATGATCTA
    GCAAGATGCC TGCGATGGGG AGGAGGTTAT GAAGGTGAGA ATTTGATGAA AATCTTAAAA
    GACATTGAAA ACAGCACAGA AATCATGTTG GACAGGTGGA AGTTTGAAGT CATACCTAAT
    GACAAAGATG AGAAGGGAGA CCCAGTGCCT TACAGTATCA TCAATAATTA TTTTTCCATT
    GGCGTGGATG CTTCCATTGC ACACAGATTC CATATCATGA GAGAGAAACA CCCAGAAAAA
    TTCAATAGTA GAATGAAGAA CAAATTTTGG TATTTTGAGT TTGGCACATC TGAAACTTTC
    TCAGCCACCT GCAAGAAGCT ACATGAATCT GTAGAAATAG AATGTGATGG AGTACAGATT
    GACTTAATAA ACATCTCTCT GGAAGGAATT GCTATTTTGA ATATACCCAG CATGCATGGA
    GGGTCCAATC TTTGGGGAGA GTCTAAGAAA AGACGAAGCC ATCGCCGAAT AGAGAAAAAA
    GGGTCTGACA AAAGGACCAC GCTCATAGAT GCCAAAGAGT TGAAGTTTGC AAGTCAAGAT
    CTGAGTGATC AGCTGCTGGA GGTGGTTGGC TTGGAAGGAG CCATGGAGAT GGGGCAAATA
    TACACAGGAC TGAAAAGTGC TGGGCGGCGG CTGGCTCAGT GCTCCTCTGT TGTCATCAGG
    ACCAGCAAGT CTCTGCCAAT GCAGATTGAT GGGGAACCAT GGATGCAGAC CCCATGCACT
    ATAAAAATTA TACACAAGAA CCAAGCCCCA ATGCTGATGG GTCCTCCTCC CAAAACCGGG
    TTGTTCTGCT CCCTCGTCAA AAGAACCAGA AACCGAAGCA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802346.1
    CDS557..2944
    Translation

    Target ORF information:

    RefSeq Version XM_010804044.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804044.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG ACTCTGCCCA CTTCAGGAGT TTCAGCCCCT
    GAGGAAAGAC AAGCGACAGT GAAAAGGGAA AAGGGTAGTT CCCAGCAGTC AAACAAAGTG
    ACCGACAAGA ACAAAATGCA AAGAGCTAAC TCTGTTACTG TGGATGGACA AGGCCTGCAG
    ATCACTCCAG TTCCTGGTAC TCATCCACTT TTAGTTTTTG TGAACCCCAA AAGTGGTGGG
    AAGCAAGGAG AACGAATTTA CAGAAAATTC CAATACCTAC TAAATCCTCG TCAGGTTTAC
    AGTCTTGCTG GAAATGGACC AATGCCAGGG TTAAACTTTT TCCGTGATGT TCCTGATTTC
    AGAGTGTTAG CCTGTGGTGG AGATGGAACT GTGGGGTGGA TTTTGGACTG CATAGAAAAG
    GCTAATGTAG TCAAGCATCC TCCAGTGGCT ATTCTGCCTC TCGGGACCGG CAATGATCTA
    GCAAGATGCC TGCGATGGGG AGGAGGTTAT GAAGGTGAGA ATTTGATGAA AATCTTAAAA
    GACATTGAAA ACAGCACAGA AATCATGTTG GACAGGTGGA AGTTTGAAGT CATACCTAAT
    GACAAAGATG AGAAGGGAGA CCCAGTGCCT TACAGTATCA TCAATAATTA TTTTTCCATT
    GGCGTGGATG CTTCCATTGC ACACAGATTC CATATCATGA GAGAGAAACA CCCAGAAAAA
    TTCAATAGTA GAATGAAGAA CAAATTTTGG TATTTTGAGT TTGGCACATC TGAAACTTTC
    TCAGCCACCT GCAAGAAGCT ACATGAATCT GTAGAAATAG AATGTGATGG AGTACAGATT
    GACTTAATAA ACATCTCTCT GGAAGGAATT GCTATTTTGA ATATACCCAG CATGCATGGA
    GGGTCCAATC TTTGGGGAGA GTCTAAGAAA AGACGAAGCC ATCGCCGAAT AGAGAAAAAA
    GGGTCTGACA AAAGGACCAC GCTCATAGAT GCCAAAGAGT TGAAGTTTGC AAGTCAAGAT
    CTGAGTGATC AGCTGCTGGA GGTGGTTGGC TTGGAAGGAG CCATGGAGAT GGGGCAAATA
    TACACAGGAC TGAAAAGTGC TGGGCGGCGG CTGGCTCAGT GCTCCTCTGT TGTCATCAGG
    ACCAGCAAGT CTCTGCCAAT GCAGATTGAT GGGGAACCAT GGATGCAGAC CCCATGCACT
    ATAAAAATTA TACACAAGAA CCAAGCCCCA ATGCTGATGG GTCCTCCTCC CAAAACCGGG
    TTGTTCTGCT CCCTCGTCAA AAGAACCAGA AACCGAAGCA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53549
    Clone ID Related Accession (Same CDS sequence) XM_010804044.3 , XM_010804044.2
    Accession Version XM_010804044.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG ACTCTGCCCA CTTCAGGAGT TTCAGCCCCT
    GAGGAAAGAC AAGCGACAGT GAAAAGGGAA AAGGGTAGTT CCCAGCAGTC AAACAAAGTG
    ACCGACAAGA ACAAAATGCA AAGAGCTAAC TCTGTTACTG TGGATGGACA AGGCCTGCAG
    ATCACTCCAG TTCCTGGTAC TCATCCACTT TTAGTTTTTG TGAACCCCAA AAGTGGTGGG
    AAGCAAGGAG AACGAATTTA CAGAAAATTC CAATACCTAC TAAATCCTCG TCAGGTTTAC
    AGTCTTGCTG GAAATGGACC AATGCCAGGG TTAAACTTTT TCCGTGATGT TCCTGATTTC
    AGAGTGTTAG CCTGTGGTGG AGATGGAACT GTGGGGTGGA TTTTGGACTG CATAGAAAAG
    GCTAATGTAG TCAAGCATCC TCCAGTGGCT ATTCTGCCTC TCGGGACCGG CAATGATCTA
    GCAAGATGCC TGCGATGGGG AGGAGGTTAT GAAGGTGAGA ATTTGATGAA AATCTTAAAA
    GACATTGAAA ACAGCACAGA AATCATGTTG GACAGGTGGA AGTTTGAAGT CATACCTAAT
    GACAAAGATG AGAAGGGAGA CCCAGTGCCT TACAGTATCA TCAATAATTA TTTTTCCATT
    GGCGTGGATG CTTCCATTGC ACACAGATTC CATATCATGA GAGAGAAACA CCCAGAAAAA
    TTCAATAGTA GAATGAAGAA CAAATTTTGG TATTTTGAGT TTGGCACATC TGAAACTTTC
    TCAGCCACCT GCAAGAAGCT ACATGAATCT GTAGAAATAG AATGTGATGG AGTACAGATT
    GACTTAATAA ACATCTCTCT GGAAGGAATT GCTATTTTGA ATATACCCAG CATGCATGGA
    GGGTCCAATC TTTGGGGAGA GTCTAAGAAA AGACGAAGCC ATCGCCGAAT AGAGAAAAAA
    GGGTCTGACA AAAGGACCAC GCTCATAGAT GCCAAAGAGT TGAAGTTTGC AAGTCAAGAT
    CTGAGTGATC AGCTGCTGGA GGTGGTTGGC TTGGAAGGAG CCATGGAGAT GGGGCAAATA
    TACACAGGAC TGAAAAGTGC TGGGCGGCGG CTGGCTCAGT GCTCCTCTGT TGTCATCAGG
    ACCAGCAAGT CTCTGCCAAT GCAGATTGAT GGGGAACCAT GGATGCAGAC CCCATGCACT
    ATAAAAATTA TACACAAGAA CCAAGCCCCA ATGCTGATGG GTCCTCCTCC CAAAACCGGG
    TTGTTCTGCT CCCTCGTCAA AAGAACCAGA AACCGAAGCA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802346.1
    CDS542..2929
    Translation

    Target ORF information:

    RefSeq Version XM_010804044.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGACA ATAGATTTTG AAGGTTTCAA GCTATTTATG
    AAGACATTCC TGGAAGCCGA GCTTCCTGAT GATTTCACAA CACACCTTTT CATGTCATTT
    AGCAACAAGT TTCCTCATTC TAGTCCAATG GTAAAAAGTA AGCCTGCTCT CCTCTCAAGT
    GGTCTGAAAA TGAACAAAGG TGCCATCACC CCTCCTCGTA CTTCTCCAGC AAACACGTGT
    TCCCCAGAAG TAATCCATCT GAAGGATATA GTCTGTTACC TGTCTCTCCT TGAAAGAGGA
    AGACCTGAGG ATAAACTGGA GTTTATGTTT CGCCTCTATG ACACAGATGG AAATGGCTTC
    CTGGACAGCT CGGAACTAGA AAATATCATC AGTCAGATGA TGCACGTGGC CGAGTACCTC
    GAATGGGACG TCACTGAACT GAATCCAATC CTCCATGAAA TGATGGAAGA AATTGACTAT
    GATCATGATG GAACAGTCTC TCTGGAGGAA TGGATTCAAG GAGGCATGAC AACAATTCCA
    CTCCTTGTGC TCCTGGGCTT AGAAAATAAT GTGAAGGATG ACGGACAGCA TGTGTGGCGA
    CTCAAGCACT TTAACAAACC TGCCTACTGC AATCTTTGCC TGAACATGCT GATCGGAGTA
    GGGAAGCAGG GCCTCTGCTG CTCCTTTTGC AAGTACACAG TCCATGAGCG CTGCGTGGCT
    AGAGCGCCTC CTTCATGTAT CAAGACTTAC GTGAAGTCCA AGAAGAACAC TGATGTGATG
    CATCATTACT GGGTTGAAGG CAACTGCCCA ACCAAGTGTG ATAAGTGCCA CAAAACGGTC
    AAATGTTACC AGGGCTTGAC GGGACTGCAT TGTGTTTGGT GTCAGATCAC ACTGCATAAT
    AAATGTGCTT CTCATCTGAA ACCTGAATGT GACTGCGGAC CTTTGAAGGA CCATATTTTA
    CCACCCACCA CAATCTGTCC AGTGGTACTG ACTCTGCCCA CTTCAGGAGT TTCAGCCCCT
    GAGGAAAGAC AAGCGACAGT GAAAAGGGAA AAGGGTAGTT CCCAGCAGTC AAACAAAGTG
    ACCGACAAGA ACAAAATGCA AAGAGCTAAC TCTGTTACTG TGGATGGACA AGGCCTGCAG
    ATCACTCCAG TTCCTGGTAC TCATCCACTT TTAGTTTTTG TGAACCCCAA AAGTGGTGGG
    AAGCAAGGAG AACGAATTTA CAGAAAATTC CAATACCTAC TAAATCCTCG TCAGGTTTAC
    AGTCTTGCTG GAAATGGACC AATGCCAGGG TTAAACTTTT TCCGTGATGT TCCTGATTTC
    AGAGTGTTAG CCTGTGGTGG AGATGGAACT GTGGGGTGGA TTTTGGACTG CATAGAAAAG
    GCTAATGTAG TCAAGCATCC TCCAGTGGCT ATTCTGCCTC TCGGGACCGG CAATGATCTA
    GCAAGATGCC TGCGATGGGG AGGAGGTTAT GAAGGTGAGA ATTTGATGAA AATCTTAAAA
    GACATTGAAA ACAGCACAGA AATCATGTTG GACAGGTGGA AGTTTGAAGT CATACCTAAT
    GACAAAGATG AGAAGGGAGA CCCAGTGCCT TACAGTATCA TCAATAATTA TTTTTCCATT
    GGCGTGGATG CTTCCATTGC ACACAGATTC CATATCATGA GAGAGAAACA CCCAGAAAAA
    TTCAATAGTA GAATGAAGAA CAAATTTTGG TATTTTGAGT TTGGCACATC TGAAACTTTC
    TCAGCCACCT GCAAGAAGCT ACATGAATCT GTAGAAATAG AATGTGATGG AGTACAGATT
    GACTTAATAA ACATCTCTCT GGAAGGAATT GCTATTTTGA ATATACCCAG CATGCATGGA
    GGGTCCAATC TTTGGGGAGA GTCTAAGAAA AGACGAAGCC ATCGCCGAAT AGAGAAAAAA
    GGGTCTGACA AAAGGACCAC GCTCATAGAT GCCAAAGAGT TGAAGTTTGC AAGTCAAGAT
    CTGAGTGATC AGCTGCTGGA GGTGGTTGGC TTGGAAGGAG CCATGGAGAT GGGGCAAATA
    TACACAGGAC TGAAAAGTGC TGGGCGGCGG CTGGCTCAGT GCTCCTCTGT TGTCATCAGG
    ACCAGCAAGT CTCTGCCAAT GCAGATTGAT GGGGAACCAT GGATGCAGAC CCCATGCACT
    ATAAAAATTA TACACAAGAA CCAAGCCCCA ATGCTGATGG GTCCTCCTCC CAAAACCGGG
    TTGTTCTGCT CCCTCGTCAA AAGAACCAGA AACCGAAGCA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53550
    Clone ID Related Accession (Same CDS sequence) XM_010804045.2
    Accession Version XM_010804045.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2376bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103887.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GGAAAGACAA
    GCGACAGTGA AAAGGGAAAA GGGTAGTTCC CAGCAGTCAA ACAAAGTGAC CGACAAGAAC
    AAAATGCAAA GAGCTAACTC TGTTACTGTG GATGGACAAG GCCTGCAGAT CACTCCAGTT
    CCTGGTACTC ATCCACTTTT AGTTTTTGTG AACCCCAAAA GTGGTGGGAA GCAAGGAGAA
    CGAATTTACA GAAAATTCCA ATACCTACTA AATCCTCGTC AGGTTTACAG TCTTGCTGGA
    AATGGACCAA TGCCAGGGTT AAACTTTTTC CGTGATGTTC CTGATTTCAG AGTGTTAGCC
    TGTGGTGGAG ATGGAACTGT GGGGTGGATT TTGGACTGCA TAGAAAAGGC TAATGTAGTC
    AAGCATCCTC CAGTGGCTAT TCTGCCTCTC GGGACCGGCA ATGATCTAGC AAGATGCCTG
    CGATGGGGAG GAGGTTATGA AGGTGAGAAT TTGATGAAAA TCTTAAAAGA CATTGAAAAC
    AGCACAGAAA TCATGTTGGA CAGGTGGAAG TTTGAAGTCA TACCTAATGA CAAAGATGAG
    AAGGGAGACC CAGTGCCTTA CAGTATCATC AATAATTATT TTTCCATTGG CGTGGATGCT
    TCCATTGCAC ACAGATTCCA TATCATGAGA GAGAAACACC CAGAAAAATT CAATAGTAGA
    ATGAAGAACA AATTTTGGTA TTTTGAGTTT GGCACATCTG AAACTTTCTC AGCCACCTGC
    AAGAAGCTAC ATGAATCTGT AGAAATAGAA TGTGATGGAG TACAGATTGA CTTAATAAAC
    ATCTCTCTGG AAGGAATTGC TATTTTGAAT ATACCCAGCA TGCATGGAGG GTCCAATCTT
    TGGGGAGAGT CTAAGAAAAG ACGAAGCCAT CGCCGAATAG AGAAAAAAGG GTCTGACAAA
    AGGACCACGC TCATAGATGC CAAAGAGTTG AAGTTTGCAA GTCAAGATCT GAGTGATCAG
    CTGCTGGAGG TGGTTGGCTT GGAAGGAGCC ATGGAGATGG GGCAAATATA CACAGGACTG
    AAAAGTGCTG GGCGGCGGCT GGCTCAGTGC TCCTCTGTTG TCATCAGGAC CAGCAAGTCT
    CTGCCAATGC AGATTGATGG GGAACCATGG ATGCAGACCC CATGCACTAT AAAAATTATA
    CACAAGAACC AAGCCCCAAT GCTGATGGGT CCTCCTCCCA AAACCGGGTT GTTCTGCTCC
    CTCGTCAAAA GAACCAGAAA CCGAAGCAAG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802347.1
    CDS542..2917
    Translation

    Target ORF information:

    RefSeq Version XM_010804045.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase beta (DGKB), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGACAAACC AGGAAAAGTG GGCCCACCTC AGCCCTTCAG AATTTTCCCA GCTTCAGAAA 
    TATGCTGAGT ATTCTACAAA GAAATTAAAA GATGTTCTTG AAGAATTCCA TGGTAATGGT
    GTGCTTGCAA AGTATAATCC TGAAGGGAAA CAAGACATTC TTAATCAAAC AATAGATTTT
    GAAGGTTTCA AGCTATTTAT GAAGACATTC CTGGAAGCCG AGCTTCCTGA TGATTTCACA
    ACACACCTTT TCATGTCATT TAGCAACAAG TTTCCTCATT CTAGTCCAAT GGTAAAAAGT
    AAGCCTGCTC TCCTCTCAAG TGGTCTGAAA ATGAACAAAG GTGCCATCAC CCCTCCTCGT
    ACTTCTCCAG CAAACACGTG TTCCCCAGAA GTAATCCATC TGAAGGATAT AGTCTGTTAC
    CTGTCTCTCC TTGAAAGAGG AAGACCTGAG GATAAACTGG AGTTTATGTT TCGCCTCTAT
    GACACAGATG GAAATGGCTT CCTGGACAGC TCGGAACTAG AAAATATCAT CAGTCAGATG
    ATGCACGTGG CCGAGTACCT CGAATGGGAC GTCACTGAAC TGAATCCAAT CCTCCATGAA
    ATGATGGAAG AAATTGACTA TGATCATGAT GGAACAGTCT CTCTGGAGGA ATGGATTCAA
    GGAGGCATGA CAACAATTCC ACTCCTTGTG CTCCTGGGCT TAGAAAATAA TGTGAAGGAT
    GACGGACAGC ATGTGTGGCG ACTCAAGCAC TTTAACAAAC CTGCCTACTG CAATCTTTGC
    CTGAACATGC TGATCGGAGT AGGGAAGCAG GGCCTCTGCT GCTCCTTTTG CAAGTACACA
    GTCCATGAGC GCTGCGTGGC TAGAGCGCCT CCTTCATGTA TCAAGACTTA CGTGAAGTCC
    AAGAAGAACA CTGATGTGAT GCATCATTAC TGGGTTGAAG GCAACTGCCC AACCAAGTGT
    GATAAGTGCC ACAAAACGGT CAAATGTTAC CAGGGCTTGA CGGGACTGCA TTGTGTTTGG
    TGTCAGATCA CACTGCATAA TAAATGTGCT TCTCATCTGA AACCTGAATG TGACTGCGGA
    CCTTTGAAGG ACCATATTTT ACCACCCACC ACAATCTGTC CAGTGGTACT GGAAAGACAA
    GCGACAGTGA AAAGGGAAAA GGGTAGTTCC CAGCAGTCAA ACAAAGTGAC CGACAAGAAC
    AAAATGCAAA GAGCTAACTC TGTTACTGTG GATGGACAAG GCCTGCAGAT CACTCCAGTT
    CCTGGTACTC ATCCACTTTT AGTTTTTGTG AACCCCAAAA GTGGTGGGAA GCAAGGAGAA
    CGAATTTACA GAAAATTCCA ATACCTACTA AATCCTCGTC AGGTTTACAG TCTTGCTGGA
    AATGGACCAA TGCCAGGGTT AAACTTTTTC CGTGATGTTC CTGATTTCAG AGTGTTAGCC
    TGTGGTGGAG ATGGAACTGT GGGGTGGATT TTGGACTGCA TAGAAAAGGC TAATGTAGTC
    AAGCATCCTC CAGTGGCTAT TCTGCCTCTC GGGACCGGCA ATGATCTAGC AAGATGCCTG
    CGATGGGGAG GAGGTTATGA AGGTGAGAAT TTGATGAAAA TCTTAAAAGA CATTGAAAAC
    AGCACAGAAA TCATGTTGGA CAGGTGGAAG TTTGAAGTCA TACCTAATGA CAAAGATGAG
    AAGGGAGACC CAGTGCCTTA CAGTATCATC AATAATTATT TTTCCATTGG CGTGGATGCT
    TCCATTGCAC ACAGATTCCA TATCATGAGA GAGAAACACC CAGAAAAATT CAATAGTAGA
    ATGAAGAACA AATTTTGGTA TTTTGAGTTT GGCACATCTG AAACTTTCTC AGCCACCTGC
    AAGAAGCTAC ATGAATCTGT AGAAATAGAA TGTGATGGAG TACAGATTGA CTTAATAAAC
    ATCTCTCTGG AAGGAATTGC TATTTTGAAT ATACCCAGCA TGCATGGAGG GTCCAATCTT
    TGGGGAGAGT CTAAGAAAAG ACGAAGCCAT CGCCGAATAG AGAAAAAAGG GTCTGACAAA
    AGGACCACGC TCATAGATGC CAAAGAGTTG AAGTTTGCAA GTCAAGATCT GAGTGATCAG
    CTGCTGGAGG TGGTTGGCTT GGAAGGAGCC ATGGAGATGG GGCAAATATA CACAGGACTG
    AAAAGTGCTG GGCGGCGGCT GGCTCAGTGC TCCTCTGTTG TCATCAGGAC CAGCAAGTCT
    CTGCCAATGC AGATTGATGG GGAACCATGG ATGCAGACCC CATGCACTAT AAAAATTATA
    CACAAGAACC AAGCCCCAAT GCTGATGGGT CCTCCTCCCA AAACCGGGTT GTTCTGCTCC
    CTCGTCAAAA GAACCAGAAA CCGAAGCAAG GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.