Qk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Qk
Entrez Gene ID	499022
Full Name	quaking
Synonyms	Qki
General protein information	Preferred Names quaking Names protein quaking homolog of mouse quaking QKI (KH domain RNA binding protein) rqkI
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001115021.1	NP_001108493.1	protein quaking

Pathways from BioSystems

Homologies

	LOC712600	XP_001101642.2
Mus musculus (house mouse)	Qk	NP_001152989.1
Gallus gallus (chicken)	QKI	NP_989641.2
Bos taurus (cattle)	QKI	NP_001007818.1
Rattus norvegicus (Norway rat)	Qk	NP_001108493.1
Danio rerio (zebrafish)	qkib	XP_005156558.1
Homo sapiens (human)	QKI	NP_006766.1
Canis lupus familiaris (dog)	QKI	NP_001003021.1
Xenopus tropicalis (tropical clawed frog)	qki	NP_001093668.1

Gene Ontology
Bibliography

Related articles in PubMed

Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1.
Ishizuka A, Hasegawa Y, Ishida K, Yanaka K, Nakagawa S
Genes to cells : devoted to molecular & cellular mechanisms19(9)704-21(2014 Sep)

Proteome analysis of spinal cord during the clinical course of monophasic experimental autoimmune encephalomyelitis.
Farias AS, Martins-de-Souza D, Guimar?es L, Pradella F, Moraes AS, Facchini G, Novello JC, Santos LM
Proteomics12(17)2656-62(2012 Aug)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

RNA binding protein QKI inhibits the ischemia/reperfusion-induced apoptosis in neonatal cardiomyocytes.
Guo W, Shi X, Liu A, Yang G, Yu F, Zheng Q, Wang Z, Allen DG, Lu Z
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28(4)593-602(2011)

Quaking regulates Hnrnpa1 expression through its 3' UTR in oligodendrocyte precursor cells.
Zearfoss NR, Clingman CC, Farley BM, McCoig LM, Ryder SP
PLoS genetics7(1)e1001269(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The overexpression of either QKI5 or QKI6 suppressed IR-induced apoptosis substantially.
Title: RNA binding protein QKI inhibits the ischemia/reperfusion-induced apoptosis in neonatal cardiomyocytes.

the QKI-6 and QKI-7 RNA binding proteins are positive regulators of PNS myelination that play a key role in Schwann cell differentiation and myelination
Title: The QKI-6 and QKI-7 RNA binding proteins block proliferation and promote Schwann cell myelination.

QKI isoforms are necessary and sufficient for promoting OPC development, which must involve direct influence of QKI on differentiation/maturation of OPCs independent of cell cycle exit
Title: The selective RNA-binding protein quaking I (QKI) is necessary and sufficient for promoting oligodendroglia differentiation.

The following Qk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Qk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42784	NM_001115021.1 Latest version!	Rattus norvegicus quaking (Qk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Qk ( NM_001115021.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001108493.1 Rattus norvegicus quaking (Qk), mRNA.

Price & Availability↑

CloneID	ORa42784
Clone ID Related Accession (Same CDS sequence)	NM_001115021.1
Accession Version	NM_001115021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	protein quaking
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC158800.1. On or before Sep 5, 2008 this sequence version replaced XM_001068844.1, XM_574316.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTCGGGG AAATGGAAAC GAAGGAGAAG CCGAAGCCCA CCCCAGATTA CTTGATGCAG 
CTGATGAACG ACAAGAAGCT TATGAGCAGC CTGCCCAACT TCTGCGGGAT CTTCAACCAC 
CTCGAGCGGC TGCTGGACGA AGAAATTAGC AGAGTACGGA AAGACATGTA CAATGACACA 
TTAAATGGCA GTACAGAGAA AAGGAGTGCA GAATTGCCTG ACGCGGTGGG ACCCATTGTT 
CAGTTACAAG AGAAACTTTA TGTGCCTGTA AAAGAATACC CTGATTTTAA TTTTGTTGGG 
AGAATCCTTG GACCTAGAGG ACTTACAGCT AAACAACTTG AAGCAGAAAC AGGATGTAAA 
ATAATGGTCC GAGGCAAAGG CTCCATGAGG GATAAAAAGA AGGAGGAGCA AAATAGAGGC 
AAACCCAATT GGGAGCATCT AAATGAAGAT TTACATGTAC TAATCACTGT GGAAGATGCT 
CAAAACAGAG CAGAAATCAA GCTGAAGAGA GCGGTTGAAG AAGTGAAGAA GTTACTGGTA 
CCTGCAGCTG AAGGAGAAGA CAGCCTGAAG AAGATGCAGC TGATGGAGCT TGCAATTCTG 
AATGGCACCT ACAGAGACGC CAACATTAAA TCACCAGCCC TTGCCTTTTC TCTTGCAGCA 
ACTGCCCAGG CTGCTCCAAG GATCATCACT GGGCCCGCGC CTGTCCTCCC ACCAGCTGCT 
CTGCGTACAC CCACGCCAGC TGGCCCTACC ATAATGCCTT TGATCAGACA GATTCAGACC 
GCTGTCATGC CAAACGGAAC TCCTCACCCA ACTGCTGCAA TAGTCCCTCC AGGGCCTGAA 
GCTGGGTTAA TCTACACACC CTATGAGTAC CCCTACACGT TGGCACCAGC TACGTCAATC 
CTTGAGTACC CCATTGAACC CAGCGGTGTG TTAGAGTGGA TTGAAATGCC AGTCATGCCT 
GATATTTCAG CCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108493.1
CDS	232..1209
Translation	MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLEWIEMPVMPDISAH

Target ORF information:

RefSeq Version	NM_001115021.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus quaking (Qk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001115021.1

ORF Insert Sequence:

ATGGTCGGGG AAATGGAAAC GAAGGAGAAG CCGAAGCCCA CCCCAGATTA CTTGATGCAG 
CTGATGAACG ACAAGAAGCT TATGAGCAGC CTGCCCAACT TCTGCGGGAT CTTCAACCAC 
CTCGAGCGGC TGCTGGACGA AGAAATTAGC AGAGTACGGA AAGACATGTA CAATGACACA 
TTAAATGGCA GTACAGAGAA AAGGAGTGCA GAATTGCCTG ACGCGGTGGG ACCCATTGTT 
CAGTTACAAG AGAAACTTTA TGTGCCTGTA AAAGAATACC CTGATTTTAA TTTTGTTGGG 
AGAATCCTTG GACCTAGAGG ACTTACAGCT AAACAACTTG AAGCAGAAAC AGGATGTAAA 
ATAATGGTCC GAGGCAAAGG CTCCATGAGG GATAAAAAGA AGGAGGAGCA AAATAGAGGC 
AAACCCAATT GGGAGCATCT AAATGAAGAT TTACATGTAC TAATCACTGT GGAAGATGCT 
CAAAACAGAG CAGAAATCAA GCTGAAGAGA GCGGTTGAAG AAGTGAAGAA GTTACTGGTA 
CCTGCAGCTG AAGGAGAAGA CAGCCTGAAG AAGATGCAGC TGATGGAGCT TGCAATTCTG 
AATGGCACCT ACAGAGACGC CAACATTAAA TCACCAGCCC TTGCCTTTTC TCTTGCAGCA 
ACTGCCCAGG CTGCTCCAAG GATCATCACT GGGCCCGCGC CTGTCCTCCC ACCAGCTGCT 
CTGCGTACAC CCACGCCAGC TGGCCCTACC ATAATGCCTT TGATCAGACA GATTCAGACC 
GCTGTCATGC CAAACGGAAC TCCTCACCCA ACTGCTGCAA TAGTCCCTCC AGGGCCTGAA 
GCTGGGTTAA TCTACACACC CTATGAGTAC CCCTACACGT TGGCACCAGC TACGTCAATC 
CTTGAGTACC CCATTGAACC CAGCGGTGTG TTAGAGTGGA TTGAAATGCC AGTCATGCCT 
GATATTTCAG CCCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1.
Genes to cells : devoted to molecular & cellular mechanisms19(9)704-21(2014 Sep)
Ishizuka A,Hasegawa Y,Ishida K,Yanaka K,Nakagawa S

Proteome analysis of spinal cord during the clinical course of monophasic experimental autoimmune encephalomyelitis.
Proteomics12(17)2656-62(2012 Aug)
Farias AS,Martins-de-Souza D,Guimar?es L,Pradella F,Moraes AS,Facchini G,Novello JC,Santos LM

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

RNA binding protein QKI inhibits the ischemia/reperfusion-induced apoptosis in neonatal cardiomyocytes.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28(4)593-602(2011)
Guo W,Shi X,Liu A,Yang G,Yu F,Zheng Q,Wang Z,Allen DG,Lu Z

Quaking regulates Hnrnpa1 expression through its 3' UTR in oligodendrocyte precursor cells.
PLoS genetics7(1)e1001269(2011 Jan)
Zearfoss NR,Clingman CC,Farley BM,McCoig LM,Ryder SP