Dgkd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dgkd
Entrez Gene ID	368088
Full Name	diacylglycerol kinase, delta
Synonyms	RGD1563309
General protein information	Preferred Names diacylglycerol kinase, delta Names diacylglycerol kinase delta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q35

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596850.1	XP_017452339.1	diacylglycerol kinase delta isoform X1
XM_017596853.1	XP_017452342.1	diacylglycerol kinase delta isoform X6
XM_017596851.1	XP_017452340.1	diacylglycerol kinase delta isoform X3
XM_006226937.3	XP_006226999.1	diacylglycerol kinase delta isoform X8
XM_017596852.1	XP_017452341.1	diacylglycerol kinase delta isoform X4
XM_006245505.3	XP_006245567.2	diacylglycerol kinase delta isoform X5
XM_017603924.1	XP_017459413.1	diacylglycerol kinase delta isoform X7
XM_006245504.3	XP_006245566.2	diacylglycerol kinase delta isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DGKD	NP_690618.2
Pan troglodytes (chimpanzee)	DGKD	XP_003309576.1
Gallus gallus (chicken)	DGKD	XP_004937002.1
Xenopus tropicalis (tropical clawed frog)	dgkd	XP_002936660.1
Rattus norvegicus (Norway rat)	Dgkd	XP_006245566.1
Mus musculus (house mouse)	Dgkd	NP_808314.2
	DGKD	XP_001114920.1
Canis lupus familiaris (dog)	DGKD	XP_005635924.1
Bos taurus (cattle)	DGKD	NP_001030359.2

Gene Ontology

The following Dgkd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dgkd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa56089	XM_017603924.1 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa62950	XM_017596850.1 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62951	XM_017596851.1 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62952	XM_017596852.1 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62953	XM_017596853.1 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa18510	XM_006226937.3 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa48908	XM_006245504.3 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa48909	XM_006245505.3 Latest version!	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dgkd ( XM_017603924.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017459413.1 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ORa56089
Clone ID Related Accession (Same CDS sequence)	XM_017603924.1
Accession Version	XM_017603924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGGACCTT GGTGTGACCA AAGTGGGCCA 
TATGAAGAGG ATCCTCTGTG GGATCAAGGA ACTGAGCCGG AGCTCCCCTG CCGCCGAGGC 
CTAGCCTCTG CATTCTCTGC CTGCATCCTC CGCTCCTCCC GAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017459413.1
CDS	16..3123
Translation	MDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPGPWCDQSGPYEEDPLWDQGTEPELPCRRGLASAFSACILRSSRD

Target ORF information:

RefSeq Version	XM_017603924.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017603924.1

ORF Insert Sequence:

ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGGACCTT GGTGTGACCA AAGTGGGCCA 
TATGAAGAGG ATCCTCTGTG GGATCAAGGA ACTGAGCCGG AGCTCCCCTG CCGCCGAGGC 
CTAGCCTCTG CATTCTCTGC CTGCATCCTC CGCTCCTCCC GAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_017596850.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452339.1 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62950
Clone ID Related Accession (Same CDS sequence)	XM_017596850.1
Accession Version	XM_017596850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGTTT GTAACTTTTC TGGGTTGGCA CATTGGTTTT GCAGCCTTAT TTTTATTTTA 
ACATTTGTGC TTAGGAAAGA AATGCAAGCA ATTTCTTCCT CTTCCAAGAC CATCCTCAAA 
GAGGGCATGC TGACCAAACA GAACAACTCG TTCCAGCGAT CGAAGAGGAG GTACTTTAAA 
CTTCGAGGGC GAACACTCTA CTATGCTAAA ACAGCAAAGT CGATTATATT TGATGAGGTA 
GACCTGACTG ATGCCAGCGT GGCAGAGTCC AGCACCAAAA ATGTCAACAA CAGTTTCACG 
GTCATCACTC CTTGTAGGAA GCTCATCTTG TGTGCTGACA ACAGGAAAGA AATGGAAGAT 
TGGATTGCAG CACTCAAGAC TGTCCAGAAC AAAGAGCATT TTGAGCCTAC CCAGTACAGC 
ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGTTCACC TCTGGGGGAC AGAGGAGGTT 
GCTGCCTGGC TGGAGCACCT CAGTCTCTGT GAGTATAAGG ACATCTTCAC GCGGCACGAC 
ATCCGGGGCT CCGAGCTCCT GCACCTGGAG CGGAGGGACC TCAAGGTACT TCACAGGTGT 
CTTCCCAGAA CCCACACCTG CACTTTACAC ACTTCTTTCA ACTATGTCCT CTTCCCTGAC 
TGTGTCCTAG CATCACTGGT CATCAGTTTT TCTTTTTGTG TATCCCAGGG TACACAGGTC 
TTTGCCTTTT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452339.1
CDS	60..3734
Translation	MIVCNFSGLAHWFCSLIFILTFVLRKEMQAISSSSKTILKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFEPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKVLHRCLPRTHTCTLHTSFNYVLFPDCVLASLVISFSFCVSQGTQVFAFY

Target ORF information:

RefSeq Version	XM_017596850.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596850.1

ORF Insert Sequence:

ATGATAGTTT GTAACTTTTC TGGGTTGGCA CATTGGTTTT GCAGCCTTAT TTTTATTTTA 
ACATTTGTGC TTAGGAAAGA AATGCAAGCA ATTTCTTCCT CTTCCAAGAC CATCCTCAAA 
GAGGGCATGC TGACCAAACA GAACAACTCG TTCCAGCGAT CGAAGAGGAG GTACTTTAAA 
CTTCGAGGGC GAACACTCTA CTATGCTAAA ACAGCAAAGT CGATTATATT TGATGAGGTA 
GACCTGACTG ATGCCAGCGT GGCAGAGTCC AGCACCAAAA ATGTCAACAA CAGTTTCACG 
GTCATCACTC CTTGTAGGAA GCTCATCTTG TGTGCTGACA ACAGGAAAGA AATGGAAGAT 
TGGATTGCAG CACTCAAGAC TGTCCAGAAC AAAGAGCATT TTGAGCCTAC CCAGTACAGC 
ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGTTCACC TCTGGGGGAC AGAGGAGGTT 
GCTGCCTGGC TGGAGCACCT CAGTCTCTGT GAGTATAAGG ACATCTTCAC GCGGCACGAC 
ATCCGGGGCT CCGAGCTCCT GCACCTGGAG CGGAGGGACC TCAAGGTACT TCACAGGTGT 
CTTCCCAGAA CCCACACCTG CACTTTACAC ACTTCTTTCA ACTATGTCCT CTTCCCTGAC 
TGTGTCCTAG CATCACTGGT CATCAGTTTT TCTTTTTGTG TATCCCAGGG TACACAGGTC 
TTTGCCTTTT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_017596851.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452340.1 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X3, mRNA.

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CloneID	ORa62951
Clone ID Related Accession (Same CDS sequence)	XM_017596851.1
Accession Version	XM_017596851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TCAAAGAGGG CATGCTGACC AAACAGAACA ACTCGTTCCA GCGATCGAAG 
AGGAGGTACT TTAAACTTCG AGGGCGAACA CTCTACTATG CTAAAACAGC AAAGTCGATT 
ATATTTGATG AGGTAGACCT GACTGATGCC AGCGTGGCAG AGTCCAGCAC CAAAAATGTC 
AACAACAGTT TCACGGTCAT CACTCCTTGT AGGAAGCTCA TCTTGTGTGC TGACAACAGG 
AAAGAAATGG AAGATTGGAT TGCAGCACTC AAGACTGTCC AGAACAAAGA GCATTTTGAG 
CCTACCCAGT ACAGCATGGA CCATTTCTCA GGGACGCATA ACTGGTACGC TTGTTCCCAC 
GCACGGCCAA CCTACTGCAA CGTGTGTCGG GAAGTTTTGT CTGGGGTCAC ATCCCATGGG 
CTATCCTGTG AAGTGTGTAA GTTTAAGGCT CACAAGCGGT GTGCTGTGCG TGCAACCAGT 
AACTGCAAGT GGACCACACT GGCCTCGATT GGAAAGGATA TCATTGAGGA CGAGGATGGG 
ATCGCCATGC CTCACCAGTG GCTGGAAGGA AACCTGCCTG TGAGTGCCAA GTGTATTGTG 
TGTGACAAGA CTTGTGGCAG CGTATTGCGC CTGCAGGACT GGCGCTGCCT CTGGTGCAAG 
GCCATGGTTC ACACATCTTG TAAAGAGTCC TTGGTGATGA AGTGTCCACT TGGCCTATGC 
AAGGTGTCTG TCATCCCTCC CACAGCACTC AACAGTATTG ACTCCGATGG GTTCTGGAAG 
GCCACCTGTC CCCCATCTTG CACAAGCCCC CTGCTGGTCT TTGTCAATTC AAAAAGTGGA 
GACAATCAAG GTGTGAAGTT TCTCAGAAGA TTCAAGCAGC TGCTGAACCC TGCCCAGGTT 
TTTGACCTCA TGAATGGAGG ACCACACCTT GGCTTACGAT TATTTCAGAA GTTTGACACC 
TTCCGGATTC TGGTTTGTGG CGGGGATGGC AGTGTTGGCT GGGTCCTCTC TGAAATTGAC 
AGCCTCAACC TTCACAAACA GTGTCAGCTA GGTGTGCTGC CCCTGGGGAC AGGGAATGAC 
CTGGCCCGCG TTCTCGGCTG GGGCTCTGCC TGTGATGACG ATACACAGCT CCCTCAGATC 
TTGGCAAAGC TGGAGAGGGC CAGCACTAAG ATGCTGGACA GGTGGAGTGT CATGGCATAT 
GAGACCAAGC TCCCTCGGCA AGCCTCCTCC TCCACGGTCA CTGAGGACTG CAGTGAGGAC 
TCAGAGGTGC AGCAAATTCT CTTCTATGAA GACTCAGTTG CAGCCCATCT TTCTAAAATC 
TTGACTTCGG ACCAACATTC TGTGGTGATC TCATCAGCCA AAGTGCTCTG TGAGACAGTG 
AAGGACTTTG TGGCTCGAGT GGGCAAGGCT TATGAGAAGA CGACAGAGAG CTCACAGGAG 
TCAGAAGTCA TGGCCAAGAA GTGCTCTGTC TTGAAAGAGA AACTGGACTC TCTCCTTAAG 
ACCTTGGATG ATGAATCTCA GGCTTCATCC TCTTTGTCCA ACCCACCTCC TACCATTGCT 
GAGGAGGCTG AGGACGGGGA TGGATCAGGC AACATCTGTA GTTCCACTGG GGACCATTTG 
GTGGGCTCAG CGTGTCCCTC CAGGCCACAG ATCTTCCGAC CCCGGGAGCA GCTAATGCTG 
AGAGCCAACA GCCTGAAGAA AGCAATTCGC CAGATTATAG AGCACACAGA AAAAGCGGTG 
GATGAGCAGA ATGCCCAGAC CCAGGAGCAG CAGGGTTTTG TCCTTGGCCT ATCTGAATCT 
GAGAAGAAGG ACTTGAAAAC TGACAATAGG GTGTGCACAT CATCTGCCCA TAGTGAGAGC 
TGTGTGGTAG CCAAGGGGAG GAGTCACCGC AAAGCATCCA GGACCCCTTG TGAAAAGCTG 
ATGAGCAAAG GTCTGTCACT TGGCAGCTCT GCTTCACTCC CTCCCAATAC AGGAAGCCGG 
GACAGCCTGC CTGCTCTGAA CACCAAGATC CTATATCCAA GTGTTCGGGC AGGGATGTCT 
GGTTCCTTGC CAGGGGGCTC AGTCATCAGC CGCTTGTTAA TTAATGCTGA TCCCTTTAAT 
GCTGAACCTG AAAACCTAGA GTACTACACA GAGAAGTGTG TCATGAACAA CTATTTTGGC 
ATTGGCTTGG ACGCAAAGAT ATCTCTAGAC TTTAACAACA AGCGTGATGA ACACCCAGAG 
AAATGCAGGA GTCGGACTAA GAACATGATG TGGTACGGTG TCCTTGGGAC CAAAGAACTG 
CTTCACAGAA CCTACAGGAA CCTGGAACAA AAGGTCCTGC TGGAGTGTGA TGGGCGCCCG 
ATTCCCCTCC CAAGTCTTCA GGGAATTGCT GTCCTCAACA TTCCCAGCTA TGCTGGAGGG 
ACCAACTTCT GGGGGGGCAC CAAGGAAGAT GATACTTTTG CAGCTCCATC ATTCGATGAT 
AAGATTCTGG AGGTGGTCGC TGTGTTCGGC AGCATGCAGA TGGCTGTGTC TCGTGTAATT 
AAGCTACAAC ATCATCGAAT TGCCCAGTGT CGCACAGTGA AGATCTCCAT CCTGGGGGAT 
GAGGGCGTGC CTGTGCAGGT GGATGGGGAA GCCTGGATCC AGCCACCTGG GTACATTCGG 
ATTGTGCACA AGAACAGGGC ACAGACTCTG ACCAGAGACA GGGCCTTTGA GAACACCCTC 
AAGTCTTGGG AAGACAAACA GAAGTGTGAG CTGCCCCGCC CACCATCCTT TTCCCTGCAC 
CCTGAGATCC TGTCTGAGGA GGAAGCGACC CAGATGGATC AGTTTGGGCA GGCAGCGGGG 
AGCCTCATTC ACAGCATTCG GGAAATAGCG CAGTCCCACA GAGACATGGA ACAGGAACTC 
GCACATGCTG TCAACGCCAG CTCCAAAGCC ATGGAGCGAG TGTACGGCAA GCCCAGAGCT 
GCAGAGGGGT TGAACTGCAG CTTTGTTCTG GAGATGGTGA ATAACATCAG AGCACTGCGC 
AGTGAGACAG AGCTGCTGCT GGCTGGGAAG ATGGCCCTGC AGTTGGATCC CCCTCAGAAG 
GAACGGCTTG GGGCTGCCCT AGTTGAGATG GACCAGCAGC TCAGGAAGCT GACAGACACT 
CCCTGGCTTT GCCAGCCCAT GGAGCCTGGT GAGGAAGAGT CTCTCCAACA GAATGTGATG 
CTGGATCTTA CTAAACGCAG CCGCAGTGGT AAATTCCGCC TTGTGACCAA GTTTAAAAAG 
GAGAAAAACA ATAAGAACAA AGAAGTTCAC AGTTACCTAG GAGCCCCTGT TCACCTCTGG 
GGGACAGAGG AGGTTGCTGC CTGGCTGGAG CACCTCAGTC TCTGTGAGTA TAAGGACATC 
TTCACGCGGC ACGACATCCG GGGCTCCGAG CTCCTGCACC TGGAGCGGAG GGACCTCAAG 
GTACTTCACA GGTGTCTTCC CAGAACCCAC ACCTGCACTT TACACACTTC TTTCAACTAT 
GTCCTCTTCC CTGACTGTGT CCTAGCATCA CTGGTCATCA GTTTTTCTTT TTGTGTATCC 
CAGGGTACAC AGGTCTTTGC CTTTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452340.1
CDS	320..3889
Translation	MTILKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFEPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKVLHRCLPRTHTCTLHTSFNYVLFPDCVLASLVISFSFCVSQGTQVFAFY

Target ORF information:

RefSeq Version	XM_017596851.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596851.1

ORF Insert Sequence:

ATGACCATCC TCAAAGAGGG CATGCTGACC AAACAGAACA ACTCGTTCCA GCGATCGAAG 
AGGAGGTACT TTAAACTTCG AGGGCGAACA CTCTACTATG CTAAAACAGC AAAGTCGATT 
ATATTTGATG AGGTAGACCT GACTGATGCC AGCGTGGCAG AGTCCAGCAC CAAAAATGTC 
AACAACAGTT TCACGGTCAT CACTCCTTGT AGGAAGCTCA TCTTGTGTGC TGACAACAGG 
AAAGAAATGG AAGATTGGAT TGCAGCACTC AAGACTGTCC AGAACAAAGA GCATTTTGAG 
CCTACCCAGT ACAGCATGGA CCATTTCTCA GGGACGCATA ACTGGTACGC TTGTTCCCAC 
GCACGGCCAA CCTACTGCAA CGTGTGTCGG GAAGTTTTGT CTGGGGTCAC ATCCCATGGG 
CTATCCTGTG AAGTGTGTAA GTTTAAGGCT CACAAGCGGT GTGCTGTGCG TGCAACCAGT 
AACTGCAAGT GGACCACACT GGCCTCGATT GGAAAGGATA TCATTGAGGA CGAGGATGGG 
ATCGCCATGC CTCACCAGTG GCTGGAAGGA AACCTGCCTG TGAGTGCCAA GTGTATTGTG 
TGTGACAAGA CTTGTGGCAG CGTATTGCGC CTGCAGGACT GGCGCTGCCT CTGGTGCAAG 
GCCATGGTTC ACACATCTTG TAAAGAGTCC TTGGTGATGA AGTGTCCACT TGGCCTATGC 
AAGGTGTCTG TCATCCCTCC CACAGCACTC AACAGTATTG ACTCCGATGG GTTCTGGAAG 
GCCACCTGTC CCCCATCTTG CACAAGCCCC CTGCTGGTCT TTGTCAATTC AAAAAGTGGA 
GACAATCAAG GTGTGAAGTT TCTCAGAAGA TTCAAGCAGC TGCTGAACCC TGCCCAGGTT 
TTTGACCTCA TGAATGGAGG ACCACACCTT GGCTTACGAT TATTTCAGAA GTTTGACACC 
TTCCGGATTC TGGTTTGTGG CGGGGATGGC AGTGTTGGCT GGGTCCTCTC TGAAATTGAC 
AGCCTCAACC TTCACAAACA GTGTCAGCTA GGTGTGCTGC CCCTGGGGAC AGGGAATGAC 
CTGGCCCGCG TTCTCGGCTG GGGCTCTGCC TGTGATGACG ATACACAGCT CCCTCAGATC 
TTGGCAAAGC TGGAGAGGGC CAGCACTAAG ATGCTGGACA GGTGGAGTGT CATGGCATAT 
GAGACCAAGC TCCCTCGGCA AGCCTCCTCC TCCACGGTCA CTGAGGACTG CAGTGAGGAC 
TCAGAGGTGC AGCAAATTCT CTTCTATGAA GACTCAGTTG CAGCCCATCT TTCTAAAATC 
TTGACTTCGG ACCAACATTC TGTGGTGATC TCATCAGCCA AAGTGCTCTG TGAGACAGTG 
AAGGACTTTG TGGCTCGAGT GGGCAAGGCT TATGAGAAGA CGACAGAGAG CTCACAGGAG 
TCAGAAGTCA TGGCCAAGAA GTGCTCTGTC TTGAAAGAGA AACTGGACTC TCTCCTTAAG 
ACCTTGGATG ATGAATCTCA GGCTTCATCC TCTTTGTCCA ACCCACCTCC TACCATTGCT 
GAGGAGGCTG AGGACGGGGA TGGATCAGGC AACATCTGTA GTTCCACTGG GGACCATTTG 
GTGGGCTCAG CGTGTCCCTC CAGGCCACAG ATCTTCCGAC CCCGGGAGCA GCTAATGCTG 
AGAGCCAACA GCCTGAAGAA AGCAATTCGC CAGATTATAG AGCACACAGA AAAAGCGGTG 
GATGAGCAGA ATGCCCAGAC CCAGGAGCAG CAGGGTTTTG TCCTTGGCCT ATCTGAATCT 
GAGAAGAAGG ACTTGAAAAC TGACAATAGG GTGTGCACAT CATCTGCCCA TAGTGAGAGC 
TGTGTGGTAG CCAAGGGGAG GAGTCACCGC AAAGCATCCA GGACCCCTTG TGAAAAGCTG 
ATGAGCAAAG GTCTGTCACT TGGCAGCTCT GCTTCACTCC CTCCCAATAC AGGAAGCCGG 
GACAGCCTGC CTGCTCTGAA CACCAAGATC CTATATCCAA GTGTTCGGGC AGGGATGTCT 
GGTTCCTTGC CAGGGGGCTC AGTCATCAGC CGCTTGTTAA TTAATGCTGA TCCCTTTAAT 
GCTGAACCTG AAAACCTAGA GTACTACACA GAGAAGTGTG TCATGAACAA CTATTTTGGC 
ATTGGCTTGG ACGCAAAGAT ATCTCTAGAC TTTAACAACA AGCGTGATGA ACACCCAGAG 
AAATGCAGGA GTCGGACTAA GAACATGATG TGGTACGGTG TCCTTGGGAC CAAAGAACTG 
CTTCACAGAA CCTACAGGAA CCTGGAACAA AAGGTCCTGC TGGAGTGTGA TGGGCGCCCG 
ATTCCCCTCC CAAGTCTTCA GGGAATTGCT GTCCTCAACA TTCCCAGCTA TGCTGGAGGG 
ACCAACTTCT GGGGGGGCAC CAAGGAAGAT GATACTTTTG CAGCTCCATC ATTCGATGAT 
AAGATTCTGG AGGTGGTCGC TGTGTTCGGC AGCATGCAGA TGGCTGTGTC TCGTGTAATT 
AAGCTACAAC ATCATCGAAT TGCCCAGTGT CGCACAGTGA AGATCTCCAT CCTGGGGGAT 
GAGGGCGTGC CTGTGCAGGT GGATGGGGAA GCCTGGATCC AGCCACCTGG GTACATTCGG 
ATTGTGCACA AGAACAGGGC ACAGACTCTG ACCAGAGACA GGGCCTTTGA GAACACCCTC 
AAGTCTTGGG AAGACAAACA GAAGTGTGAG CTGCCCCGCC CACCATCCTT TTCCCTGCAC 
CCTGAGATCC TGTCTGAGGA GGAAGCGACC CAGATGGATC AGTTTGGGCA GGCAGCGGGG 
AGCCTCATTC ACAGCATTCG GGAAATAGCG CAGTCCCACA GAGACATGGA ACAGGAACTC 
GCACATGCTG TCAACGCCAG CTCCAAAGCC ATGGAGCGAG TGTACGGCAA GCCCAGAGCT 
GCAGAGGGGT TGAACTGCAG CTTTGTTCTG GAGATGGTGA ATAACATCAG AGCACTGCGC 
AGTGAGACAG AGCTGCTGCT GGCTGGGAAG ATGGCCCTGC AGTTGGATCC CCCTCAGAAG 
GAACGGCTTG GGGCTGCCCT AGTTGAGATG GACCAGCAGC TCAGGAAGCT GACAGACACT 
CCCTGGCTTT GCCAGCCCAT GGAGCCTGGT GAGGAAGAGT CTCTCCAACA GAATGTGATG 
CTGGATCTTA CTAAACGCAG CCGCAGTGGT AAATTCCGCC TTGTGACCAA GTTTAAAAAG 
GAGAAAAACA ATAAGAACAA AGAAGTTCAC AGTTACCTAG GAGCCCCTGT TCACCTCTGG 
GGGACAGAGG AGGTTGCTGC CTGGCTGGAG CACCTCAGTC TCTGTGAGTA TAAGGACATC 
TTCACGCGGC ACGACATCCG GGGCTCCGAG CTCCTGCACC TGGAGCGGAG GGACCTCAAG 
GTACTTCACA GGTGTCTTCC CAGAACCCAC ACCTGCACTT TACACACTTC TTTCAACTAT 
GTCCTCTTCC CTGACTGTGT CCTAGCATCA CTGGTCATCA GTTTTTCTTT TTGTGTATCC 
CAGGGTACAC AGGTCTTTGC CTTTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_017596852.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452341.1 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X4, mRNA.

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CloneID	ORa62952
Clone ID Related Accession (Same CDS sequence)	XM_017596852.1
Accession Version	XM_017596852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACCA AACAGAACAA CTCGTTCCAG CGATCGAAGA GGAGGTACTT TAAACTTCGA 
GGGCGAACAC TCTACTATGC TAAAACAGCA AAGTCGATTA TATTTGATGA GGTAGACCTG 
ACTGATGCCA GCGTGGCAGA GTCCAGCACC AAAAATGTCA ACAACAGTTT CACGGTCATC 
ACTCCTTGTA GGAAGCTCAT CTTGTGTGCT GACAACAGGA AAGAAATGGA AGATTGGATT 
GCAGCACTCA AGACTGTCCA GAACAAAGAG CATTTTGAGC CTACCCAGTA CAGCATGGAC 
CATTTCTCAG GGACGCATAA CTGGTACGCT TGTTCCCACG CACGGCCAAC CTACTGCAAC 
GTGTGTCGGG AAGTTTTGTC TGGGGTCACA TCCCATGGGC TATCCTGTGA AGTGTGTAAG 
TTTAAGGCTC ACAAGCGGTG TGCTGTGCGT GCAACCAGTA ACTGCAAGTG GACCACACTG 
GCCTCGATTG GAAAGGATAT CATTGAGGAC GAGGATGGGA TCGCCATGCC TCACCAGTGG 
CTGGAAGGAA ACCTGCCTGT GAGTGCCAAG TGTATTGTGT GTGACAAGAC TTGTGGCAGC 
GTATTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCTTGT 
AAAGAGTCCT TGGTGATGAA GTGTCCACTT GGCCTATGCA AGGTGTCTGT CATCCCTCCC 
ACAGCACTCA ACAGTATTGA CTCCGATGGG TTCTGGAAGG CCACCTGTCC CCCATCTTGC 
ACAAGCCCCC TGCTGGTCTT TGTCAATTCA AAAAGTGGAG ACAATCAAGG TGTGAAGTTT 
CTCAGAAGAT TCAAGCAGCT GCTGAACCCT GCCCAGGTTT TTGACCTCAT GAATGGAGGA 
CCACACCTTG GCTTACGATT ATTTCAGAAG TTTGACACCT TCCGGATTCT GGTTTGTGGC 
GGGGATGGCA GTGTTGGCTG GGTCCTCTCT GAAATTGACA GCCTCAACCT TCACAAACAG 
TGTCAGCTAG GTGTGCTGCC CCTGGGGACA GGGAATGACC TGGCCCGCGT TCTCGGCTGG 
GGCTCTGCCT GTGATGACGA TACACAGCTC CCTCAGATCT TGGCAAAGCT GGAGAGGGCC 
AGCACTAAGA TGCTGGACAG GTGGAGTGTC ATGGCATATG AGACCAAGCT CCCTCGGCAA 
GCCTCCTCCT CCACGGTCAC TGAGGACTGC AGTGAGGACT CAGAGGTGCA GCAAATTCTC 
TTCTATGAAG ACTCAGTTGC AGCCCATCTT TCTAAAATCT TGACTTCGGA CCAACATTCT 
GTGGTGATCT CATCAGCCAA AGTGCTCTGT GAGACAGTGA AGGACTTTGT GGCTCGAGTG 
GGCAAGGCTT ATGAGAAGAC GACAGAGAGC TCACAGGAGT CAGAAGTCAT GGCCAAGAAG 
TGCTCTGTCT TGAAAGAGAA ACTGGACTCT CTCCTTAAGA CCTTGGATGA TGAATCTCAG 
GCTTCATCCT CTTTGTCCAA CCCACCTCCT ACCATTGCTG AGGAGGCTGA GGACGGGGAT 
GGATCAGGCA ACATCTGTAG TTCCACTGGG GACCATTTGG TGGGCTCAGC GTGTCCCTCC 
AGGCCACAGA TCTTCCGACC CCGGGAGCAG CTAATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGCC AGATTATAGA GCACACAGAA AAAGCGGTGG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGC AGGGTTTTGT CCTTGGCCTA TCTGAATCTG AGAAGAAGGA CTTGAAAACT 
GACAATAGGG TGTGCACATC ATCTGCCCAT AGTGAGAGCT GTGTGGTAGC CAAGGGGAGG 
AGTCACCGCA AAGCATCCAG GACCCCTTGT GAAAAGCTGA TGAGCAAAGG TCTGTCACTT 
GGCAGCTCTG CTTCACTCCC TCCCAATACA GGAAGCCGGG ACAGCCTGCC TGCTCTGAAC 
ACCAAGATCC TATATCCAAG TGTTCGGGCA GGGATGTCTG GTTCCTTGCC AGGGGGCTCA 
GTCATCAGCC GCTTGTTAAT TAATGCTGAT CCCTTTAATG CTGAACCTGA AAACCTAGAG 
TACTACACAG AGAAGTGTGT CATGAACAAC TATTTTGGCA TTGGCTTGGA CGCAAAGATA 
TCTCTAGACT TTAACAACAA GCGTGATGAA CACCCAGAGA AATGCAGGAG TCGGACTAAG 
AACATGATGT GGTACGGTGT CCTTGGGACC AAAGAACTGC TTCACAGAAC CTACAGGAAC 
CTGGAACAAA AGGTCCTGCT GGAGTGTGAT GGGCGCCCGA TTCCCCTCCC AAGTCTTCAG 
GGAATTGCTG TCCTCAACAT TCCCAGCTAT GCTGGAGGGA CCAACTTCTG GGGGGGCACC 
AAGGAAGATG ATACTTTTGC AGCTCCATCA TTCGATGATA AGATTCTGGA GGTGGTCGCT 
GTGTTCGGCA GCATGCAGAT GGCTGTGTCT CGTGTAATTA AGCTACAACA TCATCGAATT 
GCCCAGTGTC GCACAGTGAA GATCTCCATC CTGGGGGATG AGGGCGTGCC TGTGCAGGTG 
GATGGGGAAG CCTGGATCCA GCCACCTGGG TACATTCGGA TTGTGCACAA GAACAGGGCA 
CAGACTCTGA CCAGAGACAG GGCCTTTGAG AACACCCTCA AGTCTTGGGA AGACAAACAG 
AAGTGTGAGC TGCCCCGCCC ACCATCCTTT TCCCTGCACC CTGAGATCCT GTCTGAGGAG 
GAAGCGACCC AGATGGATCA GTTTGGGCAG GCAGCGGGGA GCCTCATTCA CAGCATTCGG 
GAAATAGCGC AGTCCCACAG AGACATGGAA CAGGAACTCG CACATGCTGT CAACGCCAGC 
TCCAAAGCCA TGGAGCGAGT GTACGGCAAG CCCAGAGCTG CAGAGGGGTT GAACTGCAGC 
TTTGTTCTGG AGATGGTGAA TAACATCAGA GCACTGCGCA GTGAGACAGA GCTGCTGCTG 
GCTGGGAAGA TGGCCCTGCA GTTGGATCCC CCTCAGAAGG AACGGCTTGG GGCTGCCCTA 
GTTGAGATGG ACCAGCAGCT CAGGAAGCTG ACAGACACTC CCTGGCTTTG CCAGCCCATG 
GAGCCTGGTG AGGAAGAGTC TCTCCAACAG AATGTGATGC TGGATCTTAC TAAACGCAGC 
CGCAGTGGTA AATTCCGCCT TGTGACCAAG TTTAAAAAGG AGAAAAACAA TAAGAACAAA 
GAAGTTCACA GTTACCTAGG AGCCCCTGTT CACCTCTGGG GGACAGAGGA GGTTGCTGCC 
TGGCTGGAGC ACCTCAGTCT CTGTGAGTAT AAGGACATCT TCACGCGGCA CGACATCCGG 
GGCTCCGAGC TCCTGCACCT GGAGCGGAGG GACCTCAAGG TACTTCACAG GTGTCTTCCC 
AGAACCCACA CCTGCACTTT ACACACTTCT TTCAACTATG TCCTCTTCCC TGACTGTGTC 
CTAGCATCAC TGGTCATCAG TTTTTCTTTT TGTGTATCCC AGGGTACACA GGTCTTTGCC 
TTTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452341.1
CDS	161..3709
Translation	MLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFEPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKVLHRCLPRTHTCTLHTSFNYVLFPDCVLASLVISFSFCVSQGTQVFAFY

Target ORF information:

RefSeq Version	XM_017596852.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596852.1

ORF Insert Sequence:

ATGCTGACCA AACAGAACAA CTCGTTCCAG CGATCGAAGA GGAGGTACTT TAAACTTCGA 
GGGCGAACAC TCTACTATGC TAAAACAGCA AAGTCGATTA TATTTGATGA GGTAGACCTG 
ACTGATGCCA GCGTGGCAGA GTCCAGCACC AAAAATGTCA ACAACAGTTT CACGGTCATC 
ACTCCTTGTA GGAAGCTCAT CTTGTGTGCT GACAACAGGA AAGAAATGGA AGATTGGATT 
GCAGCACTCA AGACTGTCCA GAACAAAGAG CATTTTGAGC CTACCCAGTA CAGCATGGAC 
CATTTCTCAG GGACGCATAA CTGGTACGCT TGTTCCCACG CACGGCCAAC CTACTGCAAC 
GTGTGTCGGG AAGTTTTGTC TGGGGTCACA TCCCATGGGC TATCCTGTGA AGTGTGTAAG 
TTTAAGGCTC ACAAGCGGTG TGCTGTGCGT GCAACCAGTA ACTGCAAGTG GACCACACTG 
GCCTCGATTG GAAAGGATAT CATTGAGGAC GAGGATGGGA TCGCCATGCC TCACCAGTGG 
CTGGAAGGAA ACCTGCCTGT GAGTGCCAAG TGTATTGTGT GTGACAAGAC TTGTGGCAGC 
GTATTGCGCC TGCAGGACTG GCGCTGCCTC TGGTGCAAGG CCATGGTTCA CACATCTTGT 
AAAGAGTCCT TGGTGATGAA GTGTCCACTT GGCCTATGCA AGGTGTCTGT CATCCCTCCC 
ACAGCACTCA ACAGTATTGA CTCCGATGGG TTCTGGAAGG CCACCTGTCC CCCATCTTGC 
ACAAGCCCCC TGCTGGTCTT TGTCAATTCA AAAAGTGGAG ACAATCAAGG TGTGAAGTTT 
CTCAGAAGAT TCAAGCAGCT GCTGAACCCT GCCCAGGTTT TTGACCTCAT GAATGGAGGA 
CCACACCTTG GCTTACGATT ATTTCAGAAG TTTGACACCT TCCGGATTCT GGTTTGTGGC 
GGGGATGGCA GTGTTGGCTG GGTCCTCTCT GAAATTGACA GCCTCAACCT TCACAAACAG 
TGTCAGCTAG GTGTGCTGCC CCTGGGGACA GGGAATGACC TGGCCCGCGT TCTCGGCTGG 
GGCTCTGCCT GTGATGACGA TACACAGCTC CCTCAGATCT TGGCAAAGCT GGAGAGGGCC 
AGCACTAAGA TGCTGGACAG GTGGAGTGTC ATGGCATATG AGACCAAGCT CCCTCGGCAA 
GCCTCCTCCT CCACGGTCAC TGAGGACTGC AGTGAGGACT CAGAGGTGCA GCAAATTCTC 
TTCTATGAAG ACTCAGTTGC AGCCCATCTT TCTAAAATCT TGACTTCGGA CCAACATTCT 
GTGGTGATCT CATCAGCCAA AGTGCTCTGT GAGACAGTGA AGGACTTTGT GGCTCGAGTG 
GGCAAGGCTT ATGAGAAGAC GACAGAGAGC TCACAGGAGT CAGAAGTCAT GGCCAAGAAG 
TGCTCTGTCT TGAAAGAGAA ACTGGACTCT CTCCTTAAGA CCTTGGATGA TGAATCTCAG 
GCTTCATCCT CTTTGTCCAA CCCACCTCCT ACCATTGCTG AGGAGGCTGA GGACGGGGAT 
GGATCAGGCA ACATCTGTAG TTCCACTGGG GACCATTTGG TGGGCTCAGC GTGTCCCTCC 
AGGCCACAGA TCTTCCGACC CCGGGAGCAG CTAATGCTGA GAGCCAACAG CCTGAAGAAA 
GCAATTCGCC AGATTATAGA GCACACAGAA AAAGCGGTGG ATGAGCAGAA TGCCCAGACC 
CAGGAGCAGC AGGGTTTTGT CCTTGGCCTA TCTGAATCTG AGAAGAAGGA CTTGAAAACT 
GACAATAGGG TGTGCACATC ATCTGCCCAT AGTGAGAGCT GTGTGGTAGC CAAGGGGAGG 
AGTCACCGCA AAGCATCCAG GACCCCTTGT GAAAAGCTGA TGAGCAAAGG TCTGTCACTT 
GGCAGCTCTG CTTCACTCCC TCCCAATACA GGAAGCCGGG ACAGCCTGCC TGCTCTGAAC 
ACCAAGATCC TATATCCAAG TGTTCGGGCA GGGATGTCTG GTTCCTTGCC AGGGGGCTCA 
GTCATCAGCC GCTTGTTAAT TAATGCTGAT CCCTTTAATG CTGAACCTGA AAACCTAGAG 
TACTACACAG AGAAGTGTGT CATGAACAAC TATTTTGGCA TTGGCTTGGA CGCAAAGATA 
TCTCTAGACT TTAACAACAA GCGTGATGAA CACCCAGAGA AATGCAGGAG TCGGACTAAG 
AACATGATGT GGTACGGTGT CCTTGGGACC AAAGAACTGC TTCACAGAAC CTACAGGAAC 
CTGGAACAAA AGGTCCTGCT GGAGTGTGAT GGGCGCCCGA TTCCCCTCCC AAGTCTTCAG 
GGAATTGCTG TCCTCAACAT TCCCAGCTAT GCTGGAGGGA CCAACTTCTG GGGGGGCACC 
AAGGAAGATG ATACTTTTGC AGCTCCATCA TTCGATGATA AGATTCTGGA GGTGGTCGCT 
GTGTTCGGCA GCATGCAGAT GGCTGTGTCT CGTGTAATTA AGCTACAACA TCATCGAATT 
GCCCAGTGTC GCACAGTGAA GATCTCCATC CTGGGGGATG AGGGCGTGCC TGTGCAGGTG 
GATGGGGAAG CCTGGATCCA GCCACCTGGG TACATTCGGA TTGTGCACAA GAACAGGGCA 
CAGACTCTGA CCAGAGACAG GGCCTTTGAG AACACCCTCA AGTCTTGGGA AGACAAACAG 
AAGTGTGAGC TGCCCCGCCC ACCATCCTTT TCCCTGCACC CTGAGATCCT GTCTGAGGAG 
GAAGCGACCC AGATGGATCA GTTTGGGCAG GCAGCGGGGA GCCTCATTCA CAGCATTCGG 
GAAATAGCGC AGTCCCACAG AGACATGGAA CAGGAACTCG CACATGCTGT CAACGCCAGC 
TCCAAAGCCA TGGAGCGAGT GTACGGCAAG CCCAGAGCTG CAGAGGGGTT GAACTGCAGC 
TTTGTTCTGG AGATGGTGAA TAACATCAGA GCACTGCGCA GTGAGACAGA GCTGCTGCTG 
GCTGGGAAGA TGGCCCTGCA GTTGGATCCC CCTCAGAAGG AACGGCTTGG GGCTGCCCTA 
GTTGAGATGG ACCAGCAGCT CAGGAAGCTG ACAGACACTC CCTGGCTTTG CCAGCCCATG 
GAGCCTGGTG AGGAAGAGTC TCTCCAACAG AATGTGATGC TGGATCTTAC TAAACGCAGC 
CGCAGTGGTA AATTCCGCCT TGTGACCAAG TTTAAAAAGG AGAAAAACAA TAAGAACAAA 
GAAGTTCACA GTTACCTAGG AGCCCCTGTT CACCTCTGGG GGACAGAGGA GGTTGCTGCC 
TGGCTGGAGC ACCTCAGTCT CTGTGAGTAT AAGGACATCT TCACGCGGCA CGACATCCGG 
GGCTCCGAGC TCCTGCACCT GGAGCGGAGG GACCTCAAGG TACTTCACAG GTGTCTTCCC 
AGAACCCACA CCTGCACTTT ACACACTTCT TTCAACTATG TCCTCTTCCC TGACTGTGTC 
CTAGCATCAC TGGTCATCAG TTTTTCTTTT TGTGTATCCC AGGGTACACA GGTCTTTGCC 
TTTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_017596853.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452342.1 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X7, mRNA.

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CloneID	ORa62953
Clone ID Related Accession (Same CDS sequence)	XM_017596853.1
Accession Version	XM_017596853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATT GGATTGCAGC ACTCAAGACT GTCCAGAACA AAGAGCATTT TGAGCCTACC 
CAGTACAGCA TGGACCATTT CTCAGGGACG CATAACTGGT ACGCTTGTTC CCACGCACGG 
CCAACCTACT GCAACGTGTG TCGGGAAGTT TTGTCTGGGG TCACATCCCA TGGGCTATCC 
TGTGAAGTGT GTAAGTTTAA GGCTCACAAG CGGTGTGCTG TGCGTGCAAC CAGTAACTGC 
AAGTGGACCA CACTGGCCTC GATTGGAAAG GATATCATTG AGGACGAGGA TGGGATCGCC 
ATGCCTCACC AGTGGCTGGA AGGAAACCTG CCTGTGAGTG CCAAGTGTAT TGTGTGTGAC 
AAGACTTGTG GCAGCGTATT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CTTGTAAAGA GTCCTTGGTG ATGAAGTGTC CACTTGGCCT ATGCAAGGTG 
TCTGTCATCC CTCCCACAGC ACTCAACAGT ATTGACTCCG ATGGGTTCTG GAAGGCCACC 
TGTCCCCCAT CTTGCACAAG CCCCCTGCTG GTCTTTGTCA ATTCAAAAAG TGGAGACAAT 
CAAGGTGTGA AGTTTCTCAG AAGATTCAAG CAGCTGCTGA ACCCTGCCCA GGTTTTTGAC 
CTCATGAATG GAGGACCACA CCTTGGCTTA CGATTATTTC AGAAGTTTGA CACCTTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGCAGTGTT GGCTGGGTCC TCTCTGAAAT TGACAGCCTC 
AACCTTCACA AACAGTGTCA GCTAGGTGTG CTGCCCCTGG GGACAGGGAA TGACCTGGCC 
CGCGTTCTCG GCTGGGGCTC TGCCTGTGAT GACGATACAC AGCTCCCTCA GATCTTGGCA 
AAGCTGGAGA GGGCCAGCAC TAAGATGCTG GACAGGTGGA GTGTCATGGC ATATGAGACC 
AAGCTCCCTC GGCAAGCCTC CTCCTCCACG GTCACTGAGG ACTGCAGTGA GGACTCAGAG 
GTGCAGCAAA TTCTCTTCTA TGAAGACTCA GTTGCAGCCC ATCTTTCTAA AATCTTGACT 
TCGGACCAAC ATTCTGTGGT GATCTCATCA GCCAAAGTGC TCTGTGAGAC AGTGAAGGAC 
TTTGTGGCTC GAGTGGGCAA GGCTTATGAG AAGACGACAG AGAGCTCACA GGAGTCAGAA 
GTCATGGCCA AGAAGTGCTC TGTCTTGAAA GAGAAACTGG ACTCTCTCCT TAAGACCTTG 
GATGATGAAT CTCAGGCTTC ATCCTCTTTG TCCAACCCAC CTCCTACCAT TGCTGAGGAG 
GCTGAGGACG GGGATGGATC AGGCAACATC TGTAGTTCCA CTGGGGACCA TTTGGTGGGC 
TCAGCGTGTC CCTCCAGGCC ACAGATCTTC CGACCCCGGG AGCAGCTAAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGCCAGATT ATAGAGCACA CAGAAAAAGC GGTGGATGAG 
CAGAATGCCC AGACCCAGGA GCAGCAGGGT TTTGTCCTTG GCCTATCTGA ATCTGAGAAG 
AAGGACTTGA AAACTGACAA TAGGGTGTGC ACATCATCTG CCCATAGTGA GAGCTGTGTG 
GTAGCCAAGG GGAGGAGTCA CCGCAAAGCA TCCAGGACCC CTTGTGAAAA GCTGATGAGC 
AAAGGTCTGT CACTTGGCAG CTCTGCTTCA CTCCCTCCCA ATACAGGAAG CCGGGACAGC 
CTGCCTGCTC TGAACACCAA GATCCTATAT CCAAGTGTTC GGGCAGGGAT GTCTGGTTCC 
TTGCCAGGGG GCTCAGTCAT CAGCCGCTTG TTAATTAATG CTGATCCCTT TAATGCTGAA 
CCTGAAAACC TAGAGTACTA CACAGAGAAG TGTGTCATGA ACAACTATTT TGGCATTGGC 
TTGGACGCAA AGATATCTCT AGACTTTAAC AACAAGCGTG ATGAACACCC AGAGAAATGC 
AGGAGTCGGA CTAAGAACAT GATGTGGTAC GGTGTCCTTG GGACCAAAGA ACTGCTTCAC 
AGAACCTACA GGAACCTGGA ACAAAAGGTC CTGCTGGAGT GTGATGGGCG CCCGATTCCC 
CTCCCAAGTC TTCAGGGAAT TGCTGTCCTC AACATTCCCA GCTATGCTGG AGGGACCAAC 
TTCTGGGGGG GCACCAAGGA AGATGATACT TTTGCAGCTC CATCATTCGA TGATAAGATT 
CTGGAGGTGG TCGCTGTGTT CGGCAGCATG CAGATGGCTG TGTCTCGTGT AATTAAGCTA 
CAACATCATC GAATTGCCCA GTGTCGCACA GTGAAGATCT CCATCCTGGG GGATGAGGGC 
GTGCCTGTGC AGGTGGATGG GGAAGCCTGG ATCCAGCCAC CTGGGTACAT TCGGATTGTG 
CACAAGAACA GGGCACAGAC TCTGACCAGA GACAGGGCCT TTGAGAACAC CCTCAAGTCT 
TGGGAAGACA AACAGAAGTG TGAGCTGCCC CGCCCACCAT CCTTTTCCCT GCACCCTGAG 
ATCCTGTCTG AGGAGGAAGC GACCCAGATG GATCAGTTTG GGCAGGCAGC GGGGAGCCTC 
ATTCACAGCA TTCGGGAAAT AGCGCAGTCC CACAGAGACA TGGAACAGGA ACTCGCACAT 
GCTGTCAACG CCAGCTCCAA AGCCATGGAG CGAGTGTACG GCAAGCCCAG AGCTGCAGAG 
GGGTTGAACT GCAGCTTTGT TCTGGAGATG GTGAATAACA TCAGAGCACT GCGCAGTGAG 
ACAGAGCTGC TGCTGGCTGG GAAGATGGCC CTGCAGTTGG ATCCCCCTCA GAAGGAACGG 
CTTGGGGCTG CCCTAGTTGA GATGGACCAG CAGCTCAGGA AGCTGACAGA CACTCCCTGG 
CTTTGCCAGC CCATGGAGCC TGGTGAGGAA GAGTCTCTCC AACAGAATGT GATGCTGGAT 
CTTACTAAAC GCAGCCGCAG TGGTAAATTC CGCCTTGTGA CCAAGTTTAA AAAGGAGAAA 
AACAATAAGA ACAAAGAAGT TCACAGTTAC CTAGGAGCCC CTGTTCACCT CTGGGGGACA 
GAGGAGGTTG CTGCCTGGCT GGAGCACCTC AGTCTCTGTG AGTATAAGGA CATCTTCACG 
CGGCACGACA TCCGGGGCTC CGAGCTCCTG CACCTGGAGC GGAGGGACCT CAAGGTACTT 
CACAGGTGTC TTCCCAGAAC CCACACCTGC ACTTTACACA CTTCTTTCAA CTATGTCCTC 
TTCCCTGACT GTGTCCTAGC ATCACTGGTC ATCAGTTTTT CTTTTTGTGT ATCCCAGGGT 
ACACAGGTCT TTGCCTTTTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452342.1
CDS	1908..5231
Translation	MEDWIAALKTVQNKEHFEPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKVLHRCLPRTHTCTLHTSFNYVLFPDCVLASLVISFSFCVSQGTQVFAFY

Target ORF information:

RefSeq Version	XM_017596853.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596853.1

ORF Insert Sequence:

ATGGAAGATT GGATTGCAGC ACTCAAGACT GTCCAGAACA AAGAGCATTT TGAGCCTACC 
CAGTACAGCA TGGACCATTT CTCAGGGACG CATAACTGGT ACGCTTGTTC CCACGCACGG 
CCAACCTACT GCAACGTGTG TCGGGAAGTT TTGTCTGGGG TCACATCCCA TGGGCTATCC 
TGTGAAGTGT GTAAGTTTAA GGCTCACAAG CGGTGTGCTG TGCGTGCAAC CAGTAACTGC 
AAGTGGACCA CACTGGCCTC GATTGGAAAG GATATCATTG AGGACGAGGA TGGGATCGCC 
ATGCCTCACC AGTGGCTGGA AGGAAACCTG CCTGTGAGTG CCAAGTGTAT TGTGTGTGAC 
AAGACTTGTG GCAGCGTATT GCGCCTGCAG GACTGGCGCT GCCTCTGGTG CAAGGCCATG 
GTTCACACAT CTTGTAAAGA GTCCTTGGTG ATGAAGTGTC CACTTGGCCT ATGCAAGGTG 
TCTGTCATCC CTCCCACAGC ACTCAACAGT ATTGACTCCG ATGGGTTCTG GAAGGCCACC 
TGTCCCCCAT CTTGCACAAG CCCCCTGCTG GTCTTTGTCA ATTCAAAAAG TGGAGACAAT 
CAAGGTGTGA AGTTTCTCAG AAGATTCAAG CAGCTGCTGA ACCCTGCCCA GGTTTTTGAC 
CTCATGAATG GAGGACCACA CCTTGGCTTA CGATTATTTC AGAAGTTTGA CACCTTCCGG 
ATTCTGGTTT GTGGCGGGGA TGGCAGTGTT GGCTGGGTCC TCTCTGAAAT TGACAGCCTC 
AACCTTCACA AACAGTGTCA GCTAGGTGTG CTGCCCCTGG GGACAGGGAA TGACCTGGCC 
CGCGTTCTCG GCTGGGGCTC TGCCTGTGAT GACGATACAC AGCTCCCTCA GATCTTGGCA 
AAGCTGGAGA GGGCCAGCAC TAAGATGCTG GACAGGTGGA GTGTCATGGC ATATGAGACC 
AAGCTCCCTC GGCAAGCCTC CTCCTCCACG GTCACTGAGG ACTGCAGTGA GGACTCAGAG 
GTGCAGCAAA TTCTCTTCTA TGAAGACTCA GTTGCAGCCC ATCTTTCTAA AATCTTGACT 
TCGGACCAAC ATTCTGTGGT GATCTCATCA GCCAAAGTGC TCTGTGAGAC AGTGAAGGAC 
TTTGTGGCTC GAGTGGGCAA GGCTTATGAG AAGACGACAG AGAGCTCACA GGAGTCAGAA 
GTCATGGCCA AGAAGTGCTC TGTCTTGAAA GAGAAACTGG ACTCTCTCCT TAAGACCTTG 
GATGATGAAT CTCAGGCTTC ATCCTCTTTG TCCAACCCAC CTCCTACCAT TGCTGAGGAG 
GCTGAGGACG GGGATGGATC AGGCAACATC TGTAGTTCCA CTGGGGACCA TTTGGTGGGC 
TCAGCGTGTC CCTCCAGGCC ACAGATCTTC CGACCCCGGG AGCAGCTAAT GCTGAGAGCC 
AACAGCCTGA AGAAAGCAAT TCGCCAGATT ATAGAGCACA CAGAAAAAGC GGTGGATGAG 
CAGAATGCCC AGACCCAGGA GCAGCAGGGT TTTGTCCTTG GCCTATCTGA ATCTGAGAAG 
AAGGACTTGA AAACTGACAA TAGGGTGTGC ACATCATCTG CCCATAGTGA GAGCTGTGTG 
GTAGCCAAGG GGAGGAGTCA CCGCAAAGCA TCCAGGACCC CTTGTGAAAA GCTGATGAGC 
AAAGGTCTGT CACTTGGCAG CTCTGCTTCA CTCCCTCCCA ATACAGGAAG CCGGGACAGC 
CTGCCTGCTC TGAACACCAA GATCCTATAT CCAAGTGTTC GGGCAGGGAT GTCTGGTTCC 
TTGCCAGGGG GCTCAGTCAT CAGCCGCTTG TTAATTAATG CTGATCCCTT TAATGCTGAA 
CCTGAAAACC TAGAGTACTA CACAGAGAAG TGTGTCATGA ACAACTATTT TGGCATTGGC 
TTGGACGCAA AGATATCTCT AGACTTTAAC AACAAGCGTG ATGAACACCC AGAGAAATGC 
AGGAGTCGGA CTAAGAACAT GATGTGGTAC GGTGTCCTTG GGACCAAAGA ACTGCTTCAC 
AGAACCTACA GGAACCTGGA ACAAAAGGTC CTGCTGGAGT GTGATGGGCG CCCGATTCCC 
CTCCCAAGTC TTCAGGGAAT TGCTGTCCTC AACATTCCCA GCTATGCTGG AGGGACCAAC 
TTCTGGGGGG GCACCAAGGA AGATGATACT TTTGCAGCTC CATCATTCGA TGATAAGATT 
CTGGAGGTGG TCGCTGTGTT CGGCAGCATG CAGATGGCTG TGTCTCGTGT AATTAAGCTA 
CAACATCATC GAATTGCCCA GTGTCGCACA GTGAAGATCT CCATCCTGGG GGATGAGGGC 
GTGCCTGTGC AGGTGGATGG GGAAGCCTGG ATCCAGCCAC CTGGGTACAT TCGGATTGTG 
CACAAGAACA GGGCACAGAC TCTGACCAGA GACAGGGCCT TTGAGAACAC CCTCAAGTCT 
TGGGAAGACA AACAGAAGTG TGAGCTGCCC CGCCCACCAT CCTTTTCCCT GCACCCTGAG 
ATCCTGTCTG AGGAGGAAGC GACCCAGATG GATCAGTTTG GGCAGGCAGC GGGGAGCCTC 
ATTCACAGCA TTCGGGAAAT AGCGCAGTCC CACAGAGACA TGGAACAGGA ACTCGCACAT 
GCTGTCAACG CCAGCTCCAA AGCCATGGAG CGAGTGTACG GCAAGCCCAG AGCTGCAGAG 
GGGTTGAACT GCAGCTTTGT TCTGGAGATG GTGAATAACA TCAGAGCACT GCGCAGTGAG 
ACAGAGCTGC TGCTGGCTGG GAAGATGGCC CTGCAGTTGG ATCCCCCTCA GAAGGAACGG 
CTTGGGGCTG CCCTAGTTGA GATGGACCAG CAGCTCAGGA AGCTGACAGA CACTCCCTGG 
CTTTGCCAGC CCATGGAGCC TGGTGAGGAA GAGTCTCTCC AACAGAATGT GATGCTGGAT 
CTTACTAAAC GCAGCCGCAG TGGTAAATTC CGCCTTGTGA CCAAGTTTAA AAAGGAGAAA 
AACAATAAGA ACAAAGAAGT TCACAGTTAC CTAGGAGCCC CTGTTCACCT CTGGGGGACA 
GAGGAGGTTG CTGCCTGGCT GGAGCACCTC AGTCTCTGTG AGTATAAGGA CATCTTCACG 
CGGCACGACA TCCGGGGCTC CGAGCTCCTG CACCTGGAGC GGAGGGACCT CAAGGTACTT 
CACAGGTGTC TTCCCAGAAC CCACACCTGC ACTTTACACA CTTCTTTCAA CTATGTCCTC 
TTCCCTGACT GTGTCCTAGC ATCACTGGTC ATCAGTTTTT CTTTTTGTGT ATCCCAGGGT 
ACACAGGTCT TTGCCTTTTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_006226937.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226999.1 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X10, mRNA.

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CloneID	ORa18510
Clone ID Related Accession (Same CDS sequence)	XM_006226937.3
Accession Version	XM_006226937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TCAAAGAGGG CATGCTGACC AAACAGAACA ACTCGTTCCA GCGATCGAAG 
AGGAGGTACT TTAAACTTCG AGGGCGAACA CTCTACTATG CTAAAACAGC AAAGTCGATT 
ATATTTGATG AGGTAGACCT GACTGATGCC AGCGTGGCAG AGTCCAGCAC CAAAAATGTC 
AACAACAGTT TCACGGTCAT CACTCCTTGT AGGAAGCTCA TCTTGTGTGC TGACAACAGG 
AAAGAAATGG AAGATTGGAT TGCAGCACTC AAGACTGTCC AGAACAAAGA GCACTTCCAG 
CCTACCCAGT ACAGCATGGA CCATTTCTCA GGGACGCATA ACTGGTACGC TTGTTCCCAC 
GCACGGCCAA CCTACTGCAA CGTGTGTCGG GAAGTTTTGT CTGGGGTCAC ATCCCATGGG 
CTATCCTGTG AAGTGTGTAA GTTTAAGGCT CACAAGCGGT GTGCTGTGCG TGCAACCAGT 
AACTGCAAGT GGACCACACT GGCCTCGATT GGAAAGGATA TCATTGAGGA CGAGGATGGG 
ATCGCCATGC CTCACCAGTG GCTGGAAGGA AACCTGCCTG TGAGTGCCAA GTGTATTGTG 
TGTGACAAGA CTTGTGGCAG CGTATTGCGC CTGCAGGACT GGCGCTGCCT CTGGTGCAAG 
GCCATGGTTC ACACATCTTG TAAAGAGTCC TTGGTGATGA AGTGTCCACT TGGCCTATGC 
AAGGTGTCTG TCATCCCTCC CACAGCACTC AACAGTATTG ACTCCGATGG GTTCTGGAAG 
GCCACCTGTC CCCCATCTTG CACAAGCCCC CTGCTGGTCT TTGTCAATTC AAAAAGTGGA 
GACAATCAAG GTGTGAAGTT TCTCAGAAGA TTCAAGCAGC TGCTGAACCC TGCCCAGGTT 
TTTGACCTCA TGAATGGAGG ACCACACCTT GGCTTACGAT TATTTCAGAA GTTTGACACC 
TTCCGGATTC TGGTTTGTGG CGGGGATGGC AGTGTTGGCT GGGTCCTCTC TGAAATTGAC 
AGCCTCAACC TTCACAAACA GTGTCAGCTA GGTGTGCTGC CCCTGGGGAC AGGGAATGAC 
CTGGCCCGCG TTCTCGGCTG GGGCTCTGCC TGTGATGACG ATACACAGCT CCCTCAGATC 
TTGGCAAAGC TGGAGAGGGC CAGCACTAAG ATGCTGGACA GGTGGAGTGT CATGGCATAT 
GAGACCAAGC TCCCTCGGCA AGCCTCCTCC TCCACGGTCA CTGAGGACTG CAGTGAGGAC 
TCAGAGGTGC AGCAAATTCT CTTCTATGAA GACTCAGTTG CAGCCCATCT TTCTAAAATC 
TTGACTTCGG ACCAACATTC TGTGGTGATC TCATCAGCCA AAGTGCTCTG TGAGACAGTG 
AAGGACTTTG TGGCTCGAGT GGGCAAGGCT TATGAGAAGA CGACAGAGAG CTCACAGGAG 
TCAGAAGTCA TGGCCAAGAA GTGCTCTGTC TTGAAAGAGA AACTGGACTC TCTCCTTAAG 
ACCTTGGATG ATGAATCTCA GGCTTCATCC TCTTTGTCCA ACCCACCTCC TACCATTGCT 
GAGGAGGCTG AGGACGGGGA TGGATCAGGC AACATCTGTA GTTCCACTGG GGACCATTTG 
GTGGGCTCAG CGTGTCCCTC CAGGCCACAG ATCTTCCGAC CCCGGGAGCA GCTAATGCTG 
AGAGCCAACA GCCTGAAGAA AGCAATTCGC CAGATTATAG AGCACACAGA AAAAGCGGTG 
GATGAGCAGA ATGCCCAGAC CCAGGAGCAG CAGGGTTTTG TCCTTGGCCT ATCTGAATCT 
GAGAAGAAGG ACTTGAAAAC TGACAATAGG GTGTGCACAT CATCTGCCCA TAGTGAGAGC 
TGTGTGGTAG CCAAGGGGAG GAGTCACCGC AAAGCATCCA GGACCCCTTG TGAAAAGCTG 
ATGAGCAAAG GTCTGTCACT TGGCAGCTCT GCTTCACTCC CTCCCAATAC AGGAAGCCGG 
GACAGCCTGC CTGCTCTGAA CACCAAGATC CTATATCCAA GTGTTCGGGC AGGGATGTCT 
GGTTCCTTGC CAGGGGGCTC AGTCATCAGC CGCTTGTTAA TTAATGCTGA TCCCTTTAAT 
GCTGAACCTG AAAACCTAGA GTACTACACA GAGAAGTGTG TCATGAACAA CTATTTTGGC 
ATTGGCTTGG ACGCAAAGAT ATCTCTAGAC TTTAACAACA AGCGTGATGA ACACCCAGAG 
AAATGCAGGA GTCGGACTAA GAACATGATG TGGTACGGTG TCCTTGGGAC CAAAGAACTG 
CTTCACAGAA CCTACAGGAA CCTGGAACAA AAGGTCCTGC TGGAGTGTGA TGGGCGCCCG 
ATTCCCCTCC CAAGTCTTCA GGGAATTGCT GTCCTCAACA TTCCCAGCTA TGCTGGAGGG 
ACCAACTTCT GGGGGGGCAC CAAGGAAGAT GATACTTTTG CAGCTCCATC ATTCGATGAT 
AAGATTCTGG AGGTGGTCGC TGTGTTCGGC AGCATGCAGA TGGCTGTGTC TCGTGTAATT 
AAGCTACAAC ATCATCGAAT TGCCCAGTGT CGCACAGTGA AGATCTCCAT CCTGGGGGAT 
GAGGGCGTGC CTGTGCAGGT GGATGGGGAA GCCTGGATCC AGCCACCTGG GTACATTCGG 
ATTGTGCACA AGAACAGGGC ACAGACTCTG ACCAGAGACA GGGCCTTTGA GAACACCCTC 
AAGTCTTGGG AAGACAAACA GAAGTGTGAG CTGCCCCGCC CACCATCCTT TTCCCTGCAC 
CCTGAGATCC TGTCTGAGGA GGAAGCGACC CAGATGGATC AGTTTGGGCA GGCAGCGGGG 
AGCCTCATTC ACAGCATTCG GGAAATAGCG CAGTCCCACA GAGACATGGA ACAGGAACTC 
GCACATGCTG TCAACGCCAG CTCCAAAGCC ATGGAGCGAG TGTACGGCAA GCCCAGAGCT 
GCAGAGGGGT TGAACTGCAG CTTTGTTCTG GAGATGGTGA ATAACATCAG AGCACTGCGC 
AGTGAGACAG AGCTGCTGCT GGCTGGGAAG ATGGCCCTGC AGTTGGATCC CCCTCAGAAG 
GAACGGCTTG GGGCTGCCCT AGTTGAGATG GACCAGCAGC TCAGGAAGCT GACAGACACT 
CCCTGGCTTT GCCAGCCCAT GGAGCCTGGT GAGGAAGAGT CTCTCCAACA GAATGTGATG 
CTGGATCTTA CTAAACGCAG CCGCAGTGGT AAATTCCGCC TTGTGACCAA GTTTAAAAAG 
GAGAAAAACA ATAAGAACAA AGAAGTTCAC AGTTACCTAG GAGCCCCTGT TCACCTCTGG 
GGGACAGAGG AGGTTGCTGC CTGGCTGGAG CACCTCAGTC TCTGTGAGTA TAAGGACATC 
TTCACGCGGC ACGACATCCG GGGCTCCGAG CTCCTGCACC TGGAGCGGAG GGACCTCAAG 
GACCTTGGTG TGACCAAAGT GGGCCATATG AAGAGGATCC TCTGTGGGAT CAAGGAACTG 
AGCCGGAGCT CCCCTGCCGC CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226999.1
CDS	322..3831
Translation	MTILKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFQPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSSPAAEA

Target ORF information:

RefSeq Version	XM_006226937.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226937.3

ORF Insert Sequence:

ATGACCATCC TCAAAGAGGG CATGCTGACC AAACAGAACA ACTCGTTCCA GCGATCGAAG 
AGGAGGTACT TTAAACTTCG AGGGCGAACA CTCTACTATG CTAAAACAGC AAAGTCGATT 
ATATTTGATG AGGTAGACCT GACTGATGCC AGCGTGGCAG AGTCCAGCAC CAAAAATGTC 
AACAACAGTT TCACGGTCAT CACTCCTTGT AGGAAGCTCA TCTTGTGTGC TGACAACAGG 
AAAGAAATGG AAGATTGGAT TGCAGCACTC AAGACTGTCC AGAACAAAGA GCACTTCCAG 
CCTACCCAGT ACAGCATGGA CCATTTCTCA GGGACGCATA ACTGGTACGC TTGTTCCCAC 
GCACGGCCAA CCTACTGCAA CGTGTGTCGG GAAGTTTTGT CTGGGGTCAC ATCCCATGGG 
CTATCCTGTG AAGTGTGTAA GTTTAAGGCT CACAAGCGGT GTGCTGTGCG TGCAACCAGT 
AACTGCAAGT GGACCACACT GGCCTCGATT GGAAAGGATA TCATTGAGGA CGAGGATGGG 
ATCGCCATGC CTCACCAGTG GCTGGAAGGA AACCTGCCTG TGAGTGCCAA GTGTATTGTG 
TGTGACAAGA CTTGTGGCAG CGTATTGCGC CTGCAGGACT GGCGCTGCCT CTGGTGCAAG 
GCCATGGTTC ACACATCTTG TAAAGAGTCC TTGGTGATGA AGTGTCCACT TGGCCTATGC 
AAGGTGTCTG TCATCCCTCC CACAGCACTC AACAGTATTG ACTCCGATGG GTTCTGGAAG 
GCCACCTGTC CCCCATCTTG CACAAGCCCC CTGCTGGTCT TTGTCAATTC AAAAAGTGGA 
GACAATCAAG GTGTGAAGTT TCTCAGAAGA TTCAAGCAGC TGCTGAACCC TGCCCAGGTT 
TTTGACCTCA TGAATGGAGG ACCACACCTT GGCTTACGAT TATTTCAGAA GTTTGACACC 
TTCCGGATTC TGGTTTGTGG CGGGGATGGC AGTGTTGGCT GGGTCCTCTC TGAAATTGAC 
AGCCTCAACC TTCACAAACA GTGTCAGCTA GGTGTGCTGC CCCTGGGGAC AGGGAATGAC 
CTGGCCCGCG TTCTCGGCTG GGGCTCTGCC TGTGATGACG ATACACAGCT CCCTCAGATC 
TTGGCAAAGC TGGAGAGGGC CAGCACTAAG ATGCTGGACA GGTGGAGTGT CATGGCATAT 
GAGACCAAGC TCCCTCGGCA AGCCTCCTCC TCCACGGTCA CTGAGGACTG CAGTGAGGAC 
TCAGAGGTGC AGCAAATTCT CTTCTATGAA GACTCAGTTG CAGCCCATCT TTCTAAAATC 
TTGACTTCGG ACCAACATTC TGTGGTGATC TCATCAGCCA AAGTGCTCTG TGAGACAGTG 
AAGGACTTTG TGGCTCGAGT GGGCAAGGCT TATGAGAAGA CGACAGAGAG CTCACAGGAG 
TCAGAAGTCA TGGCCAAGAA GTGCTCTGTC TTGAAAGAGA AACTGGACTC TCTCCTTAAG 
ACCTTGGATG ATGAATCTCA GGCTTCATCC TCTTTGTCCA ACCCACCTCC TACCATTGCT 
GAGGAGGCTG AGGACGGGGA TGGATCAGGC AACATCTGTA GTTCCACTGG GGACCATTTG 
GTGGGCTCAG CGTGTCCCTC CAGGCCACAG ATCTTCCGAC CCCGGGAGCA GCTAATGCTG 
AGAGCCAACA GCCTGAAGAA AGCAATTCGC CAGATTATAG AGCACACAGA AAAAGCGGTG 
GATGAGCAGA ATGCCCAGAC CCAGGAGCAG CAGGGTTTTG TCCTTGGCCT ATCTGAATCT 
GAGAAGAAGG ACTTGAAAAC TGACAATAGG GTGTGCACAT CATCTGCCCA TAGTGAGAGC 
TGTGTGGTAG CCAAGGGGAG GAGTCACCGC AAAGCATCCA GGACCCCTTG TGAAAAGCTG 
ATGAGCAAAG GTCTGTCACT TGGCAGCTCT GCTTCACTCC CTCCCAATAC AGGAAGCCGG 
GACAGCCTGC CTGCTCTGAA CACCAAGATC CTATATCCAA GTGTTCGGGC AGGGATGTCT 
GGTTCCTTGC CAGGGGGCTC AGTCATCAGC CGCTTGTTAA TTAATGCTGA TCCCTTTAAT 
GCTGAACCTG AAAACCTAGA GTACTACACA GAGAAGTGTG TCATGAACAA CTATTTTGGC 
ATTGGCTTGG ACGCAAAGAT ATCTCTAGAC TTTAACAACA AGCGTGATGA ACACCCAGAG 
AAATGCAGGA GTCGGACTAA GAACATGATG TGGTACGGTG TCCTTGGGAC CAAAGAACTG 
CTTCACAGAA CCTACAGGAA CCTGGAACAA AAGGTCCTGC TGGAGTGTGA TGGGCGCCCG 
ATTCCCCTCC CAAGTCTTCA GGGAATTGCT GTCCTCAACA TTCCCAGCTA TGCTGGAGGG 
ACCAACTTCT GGGGGGGCAC CAAGGAAGAT GATACTTTTG CAGCTCCATC ATTCGATGAT 
AAGATTCTGG AGGTGGTCGC TGTGTTCGGC AGCATGCAGA TGGCTGTGTC TCGTGTAATT 
AAGCTACAAC ATCATCGAAT TGCCCAGTGT CGCACAGTGA AGATCTCCAT CCTGGGGGAT 
GAGGGCGTGC CTGTGCAGGT GGATGGGGAA GCCTGGATCC AGCCACCTGG GTACATTCGG 
ATTGTGCACA AGAACAGGGC ACAGACTCTG ACCAGAGACA GGGCCTTTGA GAACACCCTC 
AAGTCTTGGG AAGACAAACA GAAGTGTGAG CTGCCCCGCC CACCATCCTT TTCCCTGCAC 
CCTGAGATCC TGTCTGAGGA GGAAGCGACC CAGATGGATC AGTTTGGGCA GGCAGCGGGG 
AGCCTCATTC ACAGCATTCG GGAAATAGCG CAGTCCCACA GAGACATGGA ACAGGAACTC 
GCACATGCTG TCAACGCCAG CTCCAAAGCC ATGGAGCGAG TGTACGGCAA GCCCAGAGCT 
GCAGAGGGGT TGAACTGCAG CTTTGTTCTG GAGATGGTGA ATAACATCAG AGCACTGCGC 
AGTGAGACAG AGCTGCTGCT GGCTGGGAAG ATGGCCCTGC AGTTGGATCC CCCTCAGAAG 
GAACGGCTTG GGGCTGCCCT AGTTGAGATG GACCAGCAGC TCAGGAAGCT GACAGACACT 
CCCTGGCTTT GCCAGCCCAT GGAGCCTGGT GAGGAAGAGT CTCTCCAACA GAATGTGATG 
CTGGATCTTA CTAAACGCAG CCGCAGTGGT AAATTCCGCC TTGTGACCAA GTTTAAAAAG 
GAGAAAAACA ATAAGAACAA AGAAGTTCAC AGTTACCTAG GAGCCCCTGT TCACCTCTGG 
GGGACAGAGG AGGTTGCTGC CTGGCTGGAG CACCTCAGTC TCTGTGAGTA TAAGGACATC 
TTCACGCGGC ACGACATCCG GGGCTCCGAG CTCCTGCACC TGGAGCGGAG GGACCTCAAG 
GACCTTGGTG TGACCAAAGT GGGCCATATG AAGAGGATCC TCTGTGGGAT CAAGGAACTG 
AGCCGGAGCT CCCCTGCCGC CGAGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_006245504.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245566.2 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X2, mRNA.

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CloneID	ORa48908
Clone ID Related Accession (Same CDS sequence)	XM_006245504.3
Accession Version	XM_006245504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGTTT GTAACTTTTC TGGGTTGGCA CATTGGTTTT GCAGCCTTAT TTTTATTTTA 
ACATTTGTGC TTAGGAAAGA AATGCAAGCA ATTTCTTCCT CTTCCAAGAC CATCCTCAAA 
GAGGGCATGC TGACCAAACA GAACAACTCG TTCCAGCGAT CGAAGAGGAG GTACTTTAAA 
CTTCGAGGGC GAACACTCTA CTATGCTAAA ACAGCAAAGT CGATTATATT TGATGAGGTA 
GACCTGACTG ATGCCAGCGT GGCAGAGTCC AGCACCAAAA ATGTCAACAA CAGTTTCACG 
GTCATCACTC CTTGTAGGAA GCTCATCTTG TGTGCTGACA ACAGGAAAGA AATGGAAGAT 
TGGATTGCAG CACTCAAGAC TGTCCAGAAC AAAGAGCATT TTGAGCCTAC CCAGTACAGC 
ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGTTCACC TCTGGGGGAC AGAGGAGGTT 
GCTGCCTGGC TGGAGCACCT CAGTCTCTGT GAGTATAAGG ACATCTTCAC GCGGCACGAC 
ATCCGGGGCT CCGAGCTCCT GCACCTGGAG CGGAGGGACC TCAAGGACCT TGGTGTGACC 
AAAGTGGGCC ATATGAAGAG GATCCTCTGT GGGATCAAGG AACTGAGCCG GAGCTCCCCT 
GCCGCCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245566.2
CDS	58..3672
Translation	MIVCNFSGLAHWFCSLIFILTFVLRKEMQAISSSSKTILKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFEPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSSPAAEA

Target ORF information:

RefSeq Version	XM_006245504.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245504.3

ORF Insert Sequence:

ATGATAGTTT GTAACTTTTC TGGGTTGGCA CATTGGTTTT GCAGCCTTAT TTTTATTTTA 
ACATTTGTGC TTAGGAAAGA AATGCAAGCA ATTTCTTCCT CTTCCAAGAC CATCCTCAAA 
GAGGGCATGC TGACCAAACA GAACAACTCG TTCCAGCGAT CGAAGAGGAG GTACTTTAAA 
CTTCGAGGGC GAACACTCTA CTATGCTAAA ACAGCAAAGT CGATTATATT TGATGAGGTA 
GACCTGACTG ATGCCAGCGT GGCAGAGTCC AGCACCAAAA ATGTCAACAA CAGTTTCACG 
GTCATCACTC CTTGTAGGAA GCTCATCTTG TGTGCTGACA ACAGGAAAGA AATGGAAGAT 
TGGATTGCAG CACTCAAGAC TGTCCAGAAC AAAGAGCATT TTGAGCCTAC CCAGTACAGC 
ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGTTCACC TCTGGGGGAC AGAGGAGGTT 
GCTGCCTGGC TGGAGCACCT CAGTCTCTGT GAGTATAAGG ACATCTTCAC GCGGCACGAC 
ATCCGGGGCT CCGAGCTCCT GCACCTGGAG CGGAGGGACC TCAAGGACCT TGGTGTGACC 
AAAGTGGGCC ATATGAAGAG GATCCTCTGT GGGATCAAGG AACTGAGCCG GAGCTCCCCT 
GCCGCCGAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dgkd ( XM_006245505.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245567.2 Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X5, mRNA.

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CloneID	ORa48909
Clone ID Related Accession (Same CDS sequence)	XM_006245505.3
Accession Version	XM_006245505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	diacylglycerol kinase delta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGTTT GTAACTTTTC TGGGTTGGCA CATTGGTTTT GCAGCCTTAT TTTTATTTTA 
ACATTTGTGC TTAGGAAAGA AATGCAAGCA ATTTCTTCCT CTTCCAAGAC CATCCTCAAA 
GAGGGCATGC TGACCAAACA GAACAACTCG TTCCAGCGAT CGAAGAGGAG GTACTTTAAA 
CTTCGAGGGC GAACACTCTA CTATGCTAAA ACAGCAAAGT CGATTATATT TGATGAGGTA 
GACCTGACTG ATGCCAGCGT GGCAGAGTCC AGCACCAAAA ATGTCAACAA CAGTTTCACG 
GTCATCACTC CTTGTAGGAA GCTCATCTTG TGTGCTGACA ACAGGAAAGA AATGGAAGAT 
TGGATTGCAG CACTCAAGAC TGTCCAGAAC AAAGAGCATT TTGAGCCTAC CCAGTACAGC 
ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGGACCTT GGTGTGACCA AAGTGGGCCA 
TATGAAGAGG ATCCTCTGTG GGATCAAGGA ACTGAGCCGG AGCTCCCCTG CCGCCGAGGC 
CTAGCCTCTG CATTCTCTGC CTGCATCCTC CGCTCCTCCC GAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245567.2
CDS	58..3585
Translation	MIVCNFSGLAHWFCSLIFILTFVLRKEMQAISSSSKTILKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFEPTQYSMDHFSGTHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDCSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSAHSESCVVAKGRSHRKASRTPCEKLMSKGLSLGSSASLPPNTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELPRPPSFSLHPEILSEEEATQMDQFGQAAGSLIHSIREIAQSHRDMEQELAHAVNASSKAMERVYGKPRAAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALVEMDQQLRKLTDTPWLCQPMEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSYLGAPGPWCDQSGPYEEDPLWDQGTEPELPCRRGLASAFSACILRSSRD

Target ORF information:

RefSeq Version	XM_006245505.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus diacylglycerol kinase, delta (Dgkd), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245505.3

ORF Insert Sequence:

ATGATAGTTT GTAACTTTTC TGGGTTGGCA CATTGGTTTT GCAGCCTTAT TTTTATTTTA 
ACATTTGTGC TTAGGAAAGA AATGCAAGCA ATTTCTTCCT CTTCCAAGAC CATCCTCAAA 
GAGGGCATGC TGACCAAACA GAACAACTCG TTCCAGCGAT CGAAGAGGAG GTACTTTAAA 
CTTCGAGGGC GAACACTCTA CTATGCTAAA ACAGCAAAGT CGATTATATT TGATGAGGTA 
GACCTGACTG ATGCCAGCGT GGCAGAGTCC AGCACCAAAA ATGTCAACAA CAGTTTCACG 
GTCATCACTC CTTGTAGGAA GCTCATCTTG TGTGCTGACA ACAGGAAAGA AATGGAAGAT 
TGGATTGCAG CACTCAAGAC TGTCCAGAAC AAAGAGCATT TTGAGCCTAC CCAGTACAGC 
ATGGACCATT TCTCAGGGAC GCATAACTGG TACGCTTGTT CCCACGCACG GCCAACCTAC 
TGCAACGTGT GTCGGGAAGT TTTGTCTGGG GTCACATCCC ATGGGCTATC CTGTGAAGTG 
TGTAAGTTTA AGGCTCACAA GCGGTGTGCT GTGCGTGCAA CCAGTAACTG CAAGTGGACC 
ACACTGGCCT CGATTGGAAA GGATATCATT GAGGACGAGG ATGGGATCGC CATGCCTCAC 
CAGTGGCTGG AAGGAAACCT GCCTGTGAGT GCCAAGTGTA TTGTGTGTGA CAAGACTTGT 
GGCAGCGTAT TGCGCCTGCA GGACTGGCGC TGCCTCTGGT GCAAGGCCAT GGTTCACACA 
TCTTGTAAAG AGTCCTTGGT GATGAAGTGT CCACTTGGCC TATGCAAGGT GTCTGTCATC 
CCTCCCACAG CACTCAACAG TATTGACTCC GATGGGTTCT GGAAGGCCAC CTGTCCCCCA 
TCTTGCACAA GCCCCCTGCT GGTCTTTGTC AATTCAAAAA GTGGAGACAA TCAAGGTGTG 
AAGTTTCTCA GAAGATTCAA GCAGCTGCTG AACCCTGCCC AGGTTTTTGA CCTCATGAAT 
GGAGGACCAC ACCTTGGCTT ACGATTATTT CAGAAGTTTG ACACCTTCCG GATTCTGGTT 
TGTGGCGGGG ATGGCAGTGT TGGCTGGGTC CTCTCTGAAA TTGACAGCCT CAACCTTCAC 
AAACAGTGTC AGCTAGGTGT GCTGCCCCTG GGGACAGGGA ATGACCTGGC CCGCGTTCTC 
GGCTGGGGCT CTGCCTGTGA TGACGATACA CAGCTCCCTC AGATCTTGGC AAAGCTGGAG 
AGGGCCAGCA CTAAGATGCT GGACAGGTGG AGTGTCATGG CATATGAGAC CAAGCTCCCT 
CGGCAAGCCT CCTCCTCCAC GGTCACTGAG GACTGCAGTG AGGACTCAGA GGTGCAGCAA 
ATTCTCTTCT ATGAAGACTC AGTTGCAGCC CATCTTTCTA AAATCTTGAC TTCGGACCAA 
CATTCTGTGG TGATCTCATC AGCCAAAGTG CTCTGTGAGA CAGTGAAGGA CTTTGTGGCT 
CGAGTGGGCA AGGCTTATGA GAAGACGACA GAGAGCTCAC AGGAGTCAGA AGTCATGGCC 
AAGAAGTGCT CTGTCTTGAA AGAGAAACTG GACTCTCTCC TTAAGACCTT GGATGATGAA 
TCTCAGGCTT CATCCTCTTT GTCCAACCCA CCTCCTACCA TTGCTGAGGA GGCTGAGGAC 
GGGGATGGAT CAGGCAACAT CTGTAGTTCC ACTGGGGACC ATTTGGTGGG CTCAGCGTGT 
CCCTCCAGGC CACAGATCTT CCGACCCCGG GAGCAGCTAA TGCTGAGAGC CAACAGCCTG 
AAGAAAGCAA TTCGCCAGAT TATAGAGCAC ACAGAAAAAG CGGTGGATGA GCAGAATGCC 
CAGACCCAGG AGCAGCAGGG TTTTGTCCTT GGCCTATCTG AATCTGAGAA GAAGGACTTG 
AAAACTGACA ATAGGGTGTG CACATCATCT GCCCATAGTG AGAGCTGTGT GGTAGCCAAG 
GGGAGGAGTC ACCGCAAAGC ATCCAGGACC CCTTGTGAAA AGCTGATGAG CAAAGGTCTG 
TCACTTGGCA GCTCTGCTTC ACTCCCTCCC AATACAGGAA GCCGGGACAG CCTGCCTGCT 
CTGAACACCA AGATCCTATA TCCAAGTGTT CGGGCAGGGA TGTCTGGTTC CTTGCCAGGG 
GGCTCAGTCA TCAGCCGCTT GTTAATTAAT GCTGATCCCT TTAATGCTGA ACCTGAAAAC 
CTAGAGTACT ACACAGAGAA GTGTGTCATG AACAACTATT TTGGCATTGG CTTGGACGCA 
AAGATATCTC TAGACTTTAA CAACAAGCGT GATGAACACC CAGAGAAATG CAGGAGTCGG 
ACTAAGAACA TGATGTGGTA CGGTGTCCTT GGGACCAAAG AACTGCTTCA CAGAACCTAC 
AGGAACCTGG AACAAAAGGT CCTGCTGGAG TGTGATGGGC GCCCGATTCC CCTCCCAAGT 
CTTCAGGGAA TTGCTGTCCT CAACATTCCC AGCTATGCTG GAGGGACCAA CTTCTGGGGG 
GGCACCAAGG AAGATGATAC TTTTGCAGCT CCATCATTCG ATGATAAGAT TCTGGAGGTG 
GTCGCTGTGT TCGGCAGCAT GCAGATGGCT GTGTCTCGTG TAATTAAGCT ACAACATCAT 
CGAATTGCCC AGTGTCGCAC AGTGAAGATC TCCATCCTGG GGGATGAGGG CGTGCCTGTG 
CAGGTGGATG GGGAAGCCTG GATCCAGCCA CCTGGGTACA TTCGGATTGT GCACAAGAAC 
AGGGCACAGA CTCTGACCAG AGACAGGGCC TTTGAGAACA CCCTCAAGTC TTGGGAAGAC 
AAACAGAAGT GTGAGCTGCC CCGCCCACCA TCCTTTTCCC TGCACCCTGA GATCCTGTCT 
GAGGAGGAAG CGACCCAGAT GGATCAGTTT GGGCAGGCAG CGGGGAGCCT CATTCACAGC 
ATTCGGGAAA TAGCGCAGTC CCACAGAGAC ATGGAACAGG AACTCGCACA TGCTGTCAAC 
GCCAGCTCCA AAGCCATGGA GCGAGTGTAC GGCAAGCCCA GAGCTGCAGA GGGGTTGAAC 
TGCAGCTTTG TTCTGGAGAT GGTGAATAAC ATCAGAGCAC TGCGCAGTGA GACAGAGCTG 
CTGCTGGCTG GGAAGATGGC CCTGCAGTTG GATCCCCCTC AGAAGGAACG GCTTGGGGCT 
GCCCTAGTTG AGATGGACCA GCAGCTCAGG AAGCTGACAG ACACTCCCTG GCTTTGCCAG 
CCCATGGAGC CTGGTGAGGA AGAGTCTCTC CAACAGAATG TGATGCTGGA TCTTACTAAA 
CGCAGCCGCA GTGGTAAATT CCGCCTTGTG ACCAAGTTTA AAAAGGAGAA AAACAATAAG 
AACAAAGAAG TTCACAGTTA CCTAGGAGCC CCTGGACCTT GGTGTGACCA AAGTGGGCCA 
TATGAAGAGG ATCCTCTGTG GGATCAAGGA ACTGAGCCGG AGCTCCCCTG CCGCCGAGGC 
CTAGCCTCTG CATTCTCTGC CTGCATCCTC CGCTCCTCCC GAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Dgkd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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