Zw10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zw10
Entrez Gene ID	363059
Full Name	zw10 kinetochore protein
General protein information	Preferred Names zw10 kinetochore protein Names centromere/kinetochore protein zw10 homolog ZW10 homolog, centromere/kinetochore protein ZW10, kinetochore associated, homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q23

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024801.1	NP_001019972.1	centromere/kinetochore protein zw10 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZW10	XP_508761.2
Canis lupus familiaris (dog)	ZW10	XP_536569.4
Homo sapiens (human)	ZW10	NP_004715.1
	ZW10	XP_001085694.1
Gallus gallus (chicken)	ZW10	NP_989914.1
Danio rerio (zebrafish)	zw10	NP_956435.1
Xenopus tropicalis (tropical clawed frog)	zw10	NP_001116261.1
Rattus norvegicus (Norway rat)	Zw10	NP_001019972.1
Caenorhabditis elegans (roundworm)	czw-1	NP_501327.1
Arabidopsis thaliana (thale cress)	ATZW10	NP_565757.2
Bos taurus (cattle)	ZW10	NP_001068957.1
Mus musculus (house mouse)	Zw10	NP_036169.1
Oryza sativa (rice)	Os11g0545700	NP_001068060.1

Gene Ontology
Bibliography

Related articles in PubMed

Rab33b and Rab6 are functionally overlapping regulators of Golgi homeostasis and trafficking.
Starr T, Sun Y, Wilkins N, Storrie B
Traffic (Copenhagen, Denmark)11(5)626-36(2010 May)

Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery.
Civril F, Wehenkel A, Giorgi FM, Santaguida S, Di Fonzo A, Grigorean G, Ciccarelli FD, Musacchio A
Structure (London, England : 1993)18(5)616-26(2010 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A
The Journal of cell biology185(5)859-74(2009 Jun)

Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint.
Sivaram MV, Wadzinski TL, Redick SD, Manna T, Doxsey SJ
The EMBO journal28(7)902-14(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

targeting of ZW10 to the perinuclear Golgi region was found to depend on the perinuclear accumulation of dynactin
Title: Correlation of Golgi localization of ZW10 and centrosomal accumulation of dynactin.

The following Zw10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zw10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06693	NM_001024801.1 Latest version!	Rattus norvegicus zw10 kinetochore protein (Zw10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zw10 ( NM_001024801.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019972.1 Rattus norvegicus zw10 kinetochore protein (Zw10), mRNA.

Price & Availability↑

CloneID	ORa06693
Clone ID Related Accession (Same CDS sequence)	NM_001024801.1
Accession Version	NM_001024801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	centromere/kinetochore protein zw10 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097452.1. On Jun 10, 2005 this sequence version replaced XM_343386.2. ##Evidence-Data-START## Transcript exon combination :: BC097452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTCTT TCGTGACGGA AGTCTTGGCC CACTCGGGGA GCCTGGAGAA GGAGGATCTG 
GGCACCAGGA TCAGCCGTCT GACCCGGCGG GTGGAGGAGA TCAAGGGCGA GGTGTGCAAT 
ATGATCAGCA AGAAGTACAG TGAATTCCTG CCTACCATGC AGAGCGCACA GGCCCTCGTC 
ACCCAGGTGG ACACTCTATC CAATGACATC GACCAGCTGA AATCCAGGAT AGAGACTGAG 
GTCTGTCGGG ATCTTCACAT ATCAACTGTT GAGTTTACGA ACTTGAAGCA GCGGCTGGAA 
AGAGACTCTG TCGTCCTAAA TTTGCTCAAA CAGCTGCAAG AGTTTTCTTC TGCTATTGAA 
GAGTATAACA GTGCACTGGC AGAGAAGAAA TACATCCCTG CTGCTCGGCT CCTGGAGGAG 
GCACAGGAAT GCTTGAAGTT ATTAAAATCA AAAAAATGCT TTGACTTAAA AATGTTGAAG 
TCTCTCAGCA TGGAGCTCAC GGTACAGAAG CAGAACATCC TTTACCACCT GGGAGAAGAC 
TGGCAGAAGC TGGTTGTATG GAAGTTCCCG CCATCAAAAG ATACCAGCAG CTTAGAATCT 
TGTCTACAAA CAGAACTTCA TTTATGCACT GAGCAGCCCG AGAAGGAGAT GACTCCTCTG 
CCATCCATCA GCTCTGTCCT CTTGGCGTTT TCTATTCTTG GAGAATTACC CACCAAGCTG 
AAGTCATTTG GTCAGATGCT GCTGAAGTAT ATACTTAAGC CTCTGGTGAC TTGCCCGTCG 
CTCCATGCTG TGATTGAAAG GCAGCCGAAT TCAGTTAGCA TTTGTTTCCA GTCTCTGGCG 
ACGGACTCAG AACATCCACC ACCACCCGAA GCTTTTGCAA AGATCCAGTT GGTATTGGAA 
GTGCTCCAGA AACAGCTTCT AGATTTGCCT CTTGATGCTG ACCTAGAAAT TGGAAAAGTC 
CCTGAGATCG TGTTGGCTGA GATGCTTGGT GAGGTGATCT GGGAGGACCT GTCCGATTGC 
CTCATCAGAA ACTGCTTGGT TTACTCTATT CCCACCAATA GCAGCAAGCT ACAGCAATAT 
GAGGAGATCA TACAGTCCAC AGAGGAATTT GAAAAGTCCC TAAAAGAGAT GAGATTTTTA 
AAAGGAGATA CTACTGACTT GCTGAAATAT GCTCGCAATA TCAATTCTCA TTTTGCTAAT 
AAGAAGTGCC AGGATGTGAT TGTGGCGGCT AGACATCTAA TGACCTCCGA AATTCACAAC 
ACCGTGAAGA TTGGTCCTGA TTGTGAGGAA ACCCTACCAG ACTTGCCCAG TCCTGATGCC 
GACCACAGGC TACAAGTGCA GGTGTGCAAA GTGCAGTTCA CCGATGCCGG AAACTTAGAG 
CCCGAGACTT CGCTGGACCC TCGGTCCTTC TCTTTACCCA CGTGCCGCAT CAGTGAGGCT 
GTGAAGAAGT TAATGGAGCT CGCTTATCAG ACTTTACTTG AGGCAACAAC AAGTAGCGAC 
CAATGTGCTG TCCAGCTTTT TTACTCAGTG AGAAATATCT TCCATTTGTT CCATGATGTG 
GTGCCCACAT ACCACAAGGA GAACCTTCAG AAGCTTCCCC AGTTGGCTGC CATTCACCAT 
AACAACTGCA TGTATATTGC TCACCACTTG CTGACCCTCG GCCATCAGTT CAGATCGCGG 
CTCACCCCCA TTCTTTGTGA TGGCACGACC ACTTTTGTGG ACCTTGTACC TGGCTTCAGA 
AGACTTGGGA CGGAGTGTTT TTTGGCCCAG ATGCGGACCC AGAAAGGAGA ACTTCTGGAG 
AGATTATCAA GTGCTAGAAG CTTTGCAAAC ATGGATGATG AAGAGAATTA TTCTGCAGCG 
AGCAAGGCAG TGAGGCAGGT GCTGCACCAG CTGAAGAGAC TTGGGATCGT GTGGCAGGAT 
GTCCTGCCGG TGAACATATA CTGCAAGGCT ATGGGGACCT TGCTGAACAC CGTGATTGCT 
GAGATGATTG GCAGGATCAC TGCTCTAGAG GACATCTCGA CTGAAGATGG CGATAGATTG 
TATTCCCTGT GCAAAACAGT GATGGATGAA GGGCCACAAG TATTTGCACC TCTGTCTGAT 
GAAAACAAAA ACAAGAAATA CCAAGAAGAG GTTCCGGTCT ATGTGTCCAA GTGGATGCCT 
TTCAAAGAAC TCATGATAAT GCTACAAGCT AGCTTGCAGG AAATCGGAGA TCGGTGGGCA 
GATGGAAAAG GGCCCCTGGC AACTGCATTC CCTTCCAGCG AAGTAAAAGC TTTAATTCGT 
GCCTTGTTCC AGAACACAGA AAGAAGAGCG GCTGCCCTTG CTAAGATTAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019972.1
CDS	84..2417
Translation	MASFVTEVLAHSGSLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPTMQSAQALVTQVDTLSNDIDQLKSRIETEVCRDLHISTVEFTNLKQRLERDSVVLNLLKQLQEFSSAIEEYNSALAEKKYIPAARLLEEAQECLKLLKSKKCFDLKMLKSLSMELTVQKQNILYHLGEDWQKLVVWKFPPSKDTSSLESCLQTELHLCTEQPEKEMTPLPSISSVLLAFSILGELPTKLKSFGQMLLKYILKPLVTCPSLHAVIERQPNSVSICFQSLATDSEHPPPPEAFAKIQLVLEVLQKQLLDLPLDADLEIGKVPEIVLAEMLGEVIWEDLSDCLIRNCLVYSIPTNSSKLQQYEEIIQSTEEFEKSLKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARHLMTSEIHNTVKIGPDCEETLPDLPSPDADHRLQVQVCKVQFTDAGNLEPETSLDPRSFSLPTCRISEAVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRSRLTPILCDGTTTFVDLVPGFRRLGTECFLAQMRTQKGELLERLSSARSFANMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTVIAEMIGRITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSDENKNKKYQEEVPVYVSKWMPFKELMIMLQASLQEIGDRWADGKGPLATAFPSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	NM_001024801.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zw10 kinetochore protein (Zw10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024801.1

ORF Insert Sequence:

ATGGCTTCTT TCGTGACGGA AGTCTTGGCC CACTCGGGGA GCCTGGAGAA GGAGGATCTG 
GGCACCAGGA TCAGCCGTCT GACCCGGCGG GTGGAGGAGA TCAAGGGCGA GGTGTGCAAT 
ATGATCAGCA AGAAGTACAG TGAATTCCTG CCTACCATGC AGAGCGCACA GGCCCTCGTC 
ACCCAGGTGG ACACTCTATC CAATGACATC GACCAGCTGA AATCCAGGAT AGAGACTGAG 
GTCTGTCGGG ATCTTCACAT ATCAACTGTT GAGTTTACGA ACTTGAAGCA GCGGCTGGAA 
AGAGACTCTG TCGTCCTAAA TTTGCTCAAA CAGCTGCAAG AGTTTTCTTC TGCTATTGAA 
GAGTATAACA GTGCACTGGC AGAGAAGAAA TACATCCCTG CTGCTCGGCT CCTGGAGGAG 
GCACAGGAAT GCTTGAAGTT ATTAAAATCA AAAAAATGCT TTGACTTAAA AATGTTGAAG 
TCTCTCAGCA TGGAGCTCAC GGTACAGAAG CAGAACATCC TTTACCACCT GGGAGAAGAC 
TGGCAGAAGC TGGTTGTATG GAAGTTCCCG CCATCAAAAG ATACCAGCAG CTTAGAATCT 
TGTCTACAAA CAGAACTTCA TTTATGCACT GAGCAGCCCG AGAAGGAGAT GACTCCTCTG 
CCATCCATCA GCTCTGTCCT CTTGGCGTTT TCTATTCTTG GAGAATTACC CACCAAGCTG 
AAGTCATTTG GTCAGATGCT GCTGAAGTAT ATACTTAAGC CTCTGGTGAC TTGCCCGTCG 
CTCCATGCTG TGATTGAAAG GCAGCCGAAT TCAGTTAGCA TTTGTTTCCA GTCTCTGGCG 
ACGGACTCAG AACATCCACC ACCACCCGAA GCTTTTGCAA AGATCCAGTT GGTATTGGAA 
GTGCTCCAGA AACAGCTTCT AGATTTGCCT CTTGATGCTG ACCTAGAAAT TGGAAAAGTC 
CCTGAGATCG TGTTGGCTGA GATGCTTGGT GAGGTGATCT GGGAGGACCT GTCCGATTGC 
CTCATCAGAA ACTGCTTGGT TTACTCTATT CCCACCAATA GCAGCAAGCT ACAGCAATAT 
GAGGAGATCA TACAGTCCAC AGAGGAATTT GAAAAGTCCC TAAAAGAGAT GAGATTTTTA 
AAAGGAGATA CTACTGACTT GCTGAAATAT GCTCGCAATA TCAATTCTCA TTTTGCTAAT 
AAGAAGTGCC AGGATGTGAT TGTGGCGGCT AGACATCTAA TGACCTCCGA AATTCACAAC 
ACCGTGAAGA TTGGTCCTGA TTGTGAGGAA ACCCTACCAG ACTTGCCCAG TCCTGATGCC 
GACCACAGGC TACAAGTGCA GGTGTGCAAA GTGCAGTTCA CCGATGCCGG AAACTTAGAG 
CCCGAGACTT CGCTGGACCC TCGGTCCTTC TCTTTACCCA CGTGCCGCAT CAGTGAGGCT 
GTGAAGAAGT TAATGGAGCT CGCTTATCAG ACTTTACTTG AGGCAACAAC AAGTAGCGAC 
CAATGTGCTG TCCAGCTTTT TTACTCAGTG AGAAATATCT TCCATTTGTT CCATGATGTG 
GTGCCCACAT ACCACAAGGA GAACCTTCAG AAGCTTCCCC AGTTGGCTGC CATTCACCAT 
AACAACTGCA TGTATATTGC TCACCACTTG CTGACCCTCG GCCATCAGTT CAGATCGCGG 
CTCACCCCCA TTCTTTGTGA TGGCACGACC ACTTTTGTGG ACCTTGTACC TGGCTTCAGA 
AGACTTGGGA CGGAGTGTTT TTTGGCCCAG ATGCGGACCC AGAAAGGAGA ACTTCTGGAG 
AGATTATCAA GTGCTAGAAG CTTTGCAAAC ATGGATGATG AAGAGAATTA TTCTGCAGCG 
AGCAAGGCAG TGAGGCAGGT GCTGCACCAG CTGAAGAGAC TTGGGATCGT GTGGCAGGAT 
GTCCTGCCGG TGAACATATA CTGCAAGGCT ATGGGGACCT TGCTGAACAC CGTGATTGCT 
GAGATGATTG GCAGGATCAC TGCTCTAGAG GACATCTCGA CTGAAGATGG CGATAGATTG 
TATTCCCTGT GCAAAACAGT GATGGATGAA GGGCCACAAG TATTTGCACC TCTGTCTGAT 
GAAAACAAAA ACAAGAAATA CCAAGAAGAG GTTCCGGTCT ATGTGTCCAA GTGGATGCCT 
TTCAAAGAAC TCATGATAAT GCTACAAGCT AGCTTGCAGG AAATCGGAGA TCGGTGGGCA 
GATGGAAAAG GGCCCCTGGC AACTGCATTC CCTTCCAGCG AAGTAAAAGC TTTAATTCGT 
GCCTTGTTCC AGAACACAGA AAGAAGAGCG GCTGCCCTTG CTAAGATTAA ATAG