ZW10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZW10
Entrez Gene ID	9183
Full Name	zw10 kinetochore protein
Synonyms	HZW10,KNTC1AP
General protein information	Preferred Names zw10 kinetochore protein Names centromere/kinetochore protein zw10 homolog ZW10 homolog, centromere/kinetochore protein ZW10, kinetochore associated, homolog zeste white 10 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.2
Summary	This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. [provided by RefSeq, Aug 2011].
Disorder MIM:	603954

mRNA and Protein(s)

mRNA	Protein	Name
XM_017018559.1	XP_016874048.1	centromere/kinetochore protein zw10 homolog isoform X2
NM_004724.3	NP_004715.1	centromere/kinetochore protein zw10 homolog
NM_004724.4	NP_004715.1	centromere/kinetochore protein zw10 homolog
XM_017018558.2	XP_016874047.1	centromere/kinetochore protein zw10 homolog isoform X1
XM_017018559.2	XP_016874048.1	centromere/kinetochore protein zw10 homolog isoform X2
XM_017018558.1	XP_016874047.1	centromere/kinetochore protein zw10 homolog isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZW10	XP_508761.2
Canis lupus familiaris (dog)	ZW10	XP_536569.4
Homo sapiens (human)	ZW10	NP_004715.1
	ZW10	XP_001085694.1
Gallus gallus (chicken)	ZW10	NP_989914.1
Danio rerio (zebrafish)	zw10	NP_956435.1
Xenopus tropicalis (tropical clawed frog)	zw10	NP_001116261.1
Rattus norvegicus (Norway rat)	Zw10	NP_001019972.1
Caenorhabditis elegans (roundworm)	czw-1	NP_501327.1
Arabidopsis thaliana (thale cress)	ATZW10	NP_565757.2
Bos taurus (cattle)	ZW10	NP_001068957.1
Mus musculus (house mouse)	Zw10	NP_036169.1
Oryza sativa (rice)	Os11g0545700	NP_001068060.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex.
Altenfeld A, Wohlgemuth S, Wehenkel A, Vetter IR, Musacchio A
Acta crystallographica. Section F, Structural biology communications71(Pt 4)438-42(2015 Apr)

PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the beclin?1 complex.
He S, Ni D, Ma B, Lee JH, Zhang T, Ghozalli I, Pirooz SD, Zhao Z, Bharatham N, Li B, Oh S, Lee WH, Takahashi Y, Wang HG, Minassian A, Feng P, Deretic V, Pepperkok R, Tagaya M, Yoon HS, Liang C
Nature cell biology15(10)1206-1219(2013 Oct)

hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain.
Vos LJ, Famulski JK, Chan GK
The Biochemical journal436(1)157-68(2011 May)

Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery.
Civril F, Wehenkel A, Giorgi FM, Santaguida S, Di Fonzo A, Grigorean G, Ciccarelli FD, Musacchio A
Structure (London, England : 1993)18(5)616-26(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The RZZ complex was purified to homogeneity and subjected to systemic crystallization.
Title: Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex.

hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain
Title: hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain.

Results together suggest that NAG links between p31 and ZW10-RINT-1 and is involved in Golgi-to-ER transport.
Title: Identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18 complex implicated in Golgi-to-endoplasmic reticulum retrograde transport.

The interaction between ZW10 and dynamitin showed that the N-terminal region of ZW10 is the major binding site for dynamitin and, like full-length ZW10, could move along microtubules to the centrosomal area in a dynein-dynactin-dependent manner.
Title: N-terminal region of ZW10 serves not only as a determinant for localization but also as a link with dynein function.

Stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest.
Title: Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint.

The following ZW10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZW10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04568	NM_004724.4 Latest version!	Homo sapiens zw10 kinetochore protein (ZW10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu92682	XM_017018559.2 Latest version!	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu92681	XM_017018558.2 Latest version!	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu04568	NM_004724.3	Homo sapiens zw10 kinetochore protein (ZW10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu92682	XM_017018559.1	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu92681	XM_017018558.1	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZW10 ( NM_004724.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004715.1 Homo sapiens zw10 kinetochore protein (ZW10), mRNA.

Price & Availability↑

CloneID	OHu04568
Clone ID Related Accession (Same CDS sequence)	NM_004724.3 , NM_004724.4
Accession Version	NM_004724.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	centromere/kinetochore protein zw10 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM833775.1, U54996.1, BC128264.1, DB506745.2 and BQ773926.1. On Aug 5, 2011 this sequence version replaced NM_004724.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.464687.1, SRR1803615.104543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004715.1
CDS	98..2437
Translation	MASFVTEVLAHSGRLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPSMQSAQGLITQVDKLSEDIDLLKSRIESEVRRDLHVSTGEFTDLKQQLERDSVVLSLLKQLQEFSTAIEEYNCALTEKKYVTGAQRLEEAQKCLKLLKSRKCFDLKILKSLSMELTIQKQNILYHLGEEWQKLIVWKFPPSKDTSSLESYLQTELHLYTEQSHKEEKTPMPPISSVLLAFSVLGELHSKLKSFGQMLLKYILRPLASCPSLHAVIESQPNIVIIRFESIMTNLEYPSPSEVFTKIRLVLEVLQKQLLDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	NM_004724.3
Organism	Homo sapiens(human)
Definition	Homo sapiens zw10 kinetochore protein (ZW10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004724.3

ORF Insert Sequence:

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZW10 ( NM_004724.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004715.1 Homo sapiens zw10 kinetochore protein (ZW10), mRNA.

Price & Availability↑

CloneID	OHu04568
Clone ID Related Accession (Same CDS sequence)	NM_004724.3 , NM_004724.4
Accession Version	NM_004724.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	centromere/kinetochore protein zw10 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM833775.1, U54996.1, BC128264.1, DB506745.2 and BQ773926.1. On Nov 23, 2018 this sequence version replaced NM_004724.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U54996.1, SRR1660809.133900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000200135.8/ ENSP00000200135.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004715.1
CDS	27..2366
Translation	MASFVTEVLAHSGRLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPSMQSAQGLITQVDKLSEDIDLLKSRIESEVRRDLHVSTGEFTDLKQQLERDSVVLSLLKQLQEFSTAIEEYNCALTEKKYVTGAQRLEEAQKCLKLLKSRKCFDLKILKSLSMELTIQKQNILYHLGEEWQKLIVWKFPPSKDTSSLESYLQTELHLYTEQSHKEEKTPMPPISSVLLAFSVLGELHSKLKSFGQMLLKYILRPLASCPSLHAVIESQPNIVIIRFESIMTNLEYPSPSEVFTKIRLVLEVLQKQLLDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	NM_004724.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zw10 kinetochore protein (ZW10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004724.4

ORF Insert Sequence:

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZW10 ( XM_017018559.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874048.1 Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92682
Clone ID Related Accession (Same CDS sequence)	XM_017018559.1 , XM_017018559.2
Accession Version	XM_017018559.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centromere/kinetochore protein zw10 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874048.1
CDS	714..2393
Translation	MPPISSVLLAFSVLGELHSKLKSFGQMLLKYILRPLASCPSLHAVIESQPNIVIIRFESIMTNLEYPSPSEVFTKIRLVLEVLQKQLLDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	XM_017018559.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018559.1

ORF Insert Sequence:

ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZW10 ( XM_017018559.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874048.1 Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92682
Clone ID Related Accession (Same CDS sequence)	XM_017018559.1 , XM_017018559.2
Accession Version	XM_017018559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere/kinetochore protein zw10 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874048.1
CDS	710..2389
Translation	MPPISSVLLAFSVLGELHSKLKSFGQMLLKYILRPLASCPSLHAVIESQPNIVIIRFESIMTNLEYPSPSEVFTKIRLVLEVLQKQLLDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	XM_017018559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018559.2

ORF Insert Sequence:

ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGGTCAGAT GCTGCTGAAG TATATCCTTA GGCCGCTGGC ATCTTGCCCA 
TCCCTTCATG CTGTGATAGA AAGCCAGCCT AACATAGTTA TTATTCGTTT TGAATCTATA 
ATGACTAACT TGGAATATCC ATCACCATCT GAAGTTTTTA CAAAGATCAG ACTGGTACTA 
GAAGTGCTCC AGAAACAGCT TCTAGATTTG CCACTTGACA CTGACCTGGA AAATGAAAAA 
ACATCTACTG TCCCATTGGC TGAGATGCTT GGAGACATGA TCTGGGAGGA CTTGTCTGAG 
TGCCTCATCA AAAACTGTTT GGTTTATTCG ATTCCAACAA ATAGCAGCAA ATTACAGCAA 
TATGAAGAGA TCATACAGTC CACTGAAGAA TTTGAAAATG CCCTAAAGGA AATGAGATTT 
TTAAAAGGAG ATACTACAGA TTTGCTGAAA TACGCTCGTA ACATCAATTC TCATTTTGCA 
AACAAAAAGT GCCAGGATGT GATTGTGGCA GCCAGAAATC TAATGACCTC AGAAATTCAT 
AACACTGTGA AGATTATTCC TGATTCTAAG ATAAATGTGC CAGAGTTACC CACTCCTGAT 
GAGGATAACA AACTGGAAGT ACAGAAAGTA TCCAATACTC AGTACCACGA AGTGATGAAT 
TTAGAGCCTG AAAATACATT GGACCAACAT TCCTTTTCCT TGCCCACATG CCGTATCAGT 
GAGTCTGTGA AGAAATTAAT GGAACTCGCC TATCAGACTT TACTAGAGGC AACAACCAGT 
AGTGATCAAT GTGCTGTTCA ACTTTTCTAC TCAGTGAGGA ATATCTTCCA TTTGTTCCAT 
GATGTTGTAC CAACATATCA CAAGGAGAAC CTTCAAAAAC TTCCCCAGTT GGCTGCTATT 
CATCACAACA ACTGTATGTA CATTGCTCAC CACTTGCTGA CCCTCGGGCA TCAGTTCAGA 
TTGCGTCTTG CCCCCATTCT TTGTGATGGC ACTGCTACTT TTGTGGATCT TGTACCTGGC 
TTCAGGAGAC TTGGGACAGA ATGCTTTTTG GCCCAAATGC GGGCACAGAA AGGTGAACTT 
CTGGAAAGAT TATCAAGTGC TAGGAACTTT TCAAATATGG ACGATGAAGA GAATTATTCT 
GCAGCAAGTA AAGCAGTCCG GCAGGTACTG CACCAACTAA AGAGACTTGG AATTGTGTGG 
CAGGATGTCC TGCCAGTGAA TATATATTGC AAGGCTATGG GGACTTTACT CAATACAGCA 
ATTTCTGAGG TCATTGGCAA AATTACTGCC CTAGAGGACA TATCTACTGA AGATGGTGAT 
AGGTTATATT CCTTATGCAA AACAGTGATG GATGAAGGAC CCCAAGTATT TGCACCTTTA 
TCTGAAGAAA GCAAGAACAA GAAATATCAA GAAGAGGTTC CAGTCTATGT GCCAAAATGG 
ATGCCATTCA AGGAATTGAT GATGATGCTA CAAGCCAGCT TGCAAGAAAT TGGGGATCGG 
TGGGCAGATG GAAAAGGACC CCTGGCAGCT GCGTTCTCTT CCAGTGAAGT AAAAGCTTTA 
ATTCGTGCCT TGTTTCAGAA CACAGAAAGA AGAGCAGCTG CCCTTGCTAA AATTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZW10 ( XM_017018558.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874047.1 Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu92681
Clone ID Related Accession (Same CDS sequence)	XM_017018558.2 , XM_017018558.1
Accession Version	XM_017018558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centromere/kinetochore protein zw10 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGATTTGCC ACTTGACACT GACCTGGAAA ATGAAAAAAC ATCTACTGTC 
CCATTGGCTG AGATGCTTGG AGACATGATC TGGGAGGACT TGTCTGAGTG CCTCATCAAA 
AACTGTTTGG TTTATTCGAT TCCAACAAAT AGCAGCAAAT TACAGCAATA TGAAGAGATC 
ATACAGTCCA CTGAAGAATT TGAAAATGCC CTAAAGGAAA TGAGATTTTT AAAAGGAGAT 
ACTACAGATT TGCTGAAATA CGCTCGTAAC ATCAATTCTC ATTTTGCAAA CAAAAAGTGC 
CAGGATGTGA TTGTGGCAGC CAGAAATCTA ATGACCTCAG AAATTCATAA CACTGTGAAG 
ATTATTCCTG ATTCTAAGAT AAATGTGCCA GAGTTACCCA CTCCTGATGA GGATAACAAA 
CTGGAAGTAC AGAAAGTATC CAATACTCAG TACCACGAAG TGATGAATTT AGAGCCTGAA 
AATACATTGG ACCAACATTC CTTTTCCTTG CCCACATGCC GTATCAGTGA GTCTGTGAAG 
AAATTAATGG AACTCGCCTA TCAGACTTTA CTAGAGGCAA CAACCAGTAG TGATCAATGT 
GCTGTTCAAC TTTTCTACTC AGTGAGGAAT ATCTTCCATT TGTTCCATGA TGTTGTACCA 
ACATATCACA AGGAGAACCT TCAAAAACTT CCCCAGTTGG CTGCTATTCA TCACAACAAC 
TGTATGTACA TTGCTCACCA CTTGCTGACC CTCGGGCATC AGTTCAGATT GCGTCTTGCC 
CCCATTCTTT GTGATGGCAC TGCTACTTTT GTGGATCTTG TACCTGGCTT CAGGAGACTT 
GGGACAGAAT GCTTTTTGGC CCAAATGCGG GCACAGAAAG GTGAACTTCT GGAAAGATTA 
TCAAGTGCTA GGAACTTTTC AAATATGGAC GATGAAGAGA ATTATTCTGC AGCAAGTAAA 
GCAGTCCGGC AGGTACTGCA CCAACTAAAG AGACTTGGAA TTGTGTGGCA GGATGTCCTG 
CCAGTGAATA TATATTGCAA GGCTATGGGG ACTTTACTCA ATACAGCAAT TTCTGAGGTC 
ATTGGCAAAA TTACTGCCCT AGAGGACATA TCTACTGAAG ATGGTGATAG GTTATATTCC 
TTATGCAAAA CAGTGATGGA TGAAGGACCC CAAGTATTTG CACCTTTATC TGAAGAAAGC 
AAGAACAAGA AATATCAAGA AGAGGTTCCA GTCTATGTGC CAAAATGGAT GCCATTCAAG 
GAATTGATGA TGATGCTACA AGCCAGCTTG CAAGAAATTG GGGATCGGTG GGCAGATGGA 
AAAGGACCCC TGGCAGCTGC GTTCTCTTCC AGTGAAGTAA AAGCTTTAAT TCGTGCCTTG 
TTTCAGAACA CAGAAAGAAG AGCAGCTGCC CTTGCTAAAA TTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874047.1
CDS	34..2181
Translation	MASFVTEVLAHSGRLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPSMQSAQGLITQVDKLSEDIDLLKSRIESEVRRDLHVSTGEFTDLKQQLERDSVVLSLLKQLQEFSTAIEEYNCALTEKKYVTGAQRLEEAQKCLKLLKSRKCFDLKILKSLSMELTIQKQNILYHLGEEWQKLIVWKFPPSKDTSSLESYLQTELHLYTEQSHKEEKTPMPPISSVLLAFSVLGELHSKLKSFDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	XM_017018558.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018558.2

ORF Insert Sequence:

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGATTTGCC ACTTGACACT GACCTGGAAA ATGAAAAAAC ATCTACTGTC 
CCATTGGCTG AGATGCTTGG AGACATGATC TGGGAGGACT TGTCTGAGTG CCTCATCAAA 
AACTGTTTGG TTTATTCGAT TCCAACAAAT AGCAGCAAAT TACAGCAATA TGAAGAGATC 
ATACAGTCCA CTGAAGAATT TGAAAATGCC CTAAAGGAAA TGAGATTTTT AAAAGGAGAT 
ACTACAGATT TGCTGAAATA CGCTCGTAAC ATCAATTCTC ATTTTGCAAA CAAAAAGTGC 
CAGGATGTGA TTGTGGCAGC CAGAAATCTA ATGACCTCAG AAATTCATAA CACTGTGAAG 
ATTATTCCTG ATTCTAAGAT AAATGTGCCA GAGTTACCCA CTCCTGATGA GGATAACAAA 
CTGGAAGTAC AGAAAGTATC CAATACTCAG TACCACGAAG TGATGAATTT AGAGCCTGAA 
AATACATTGG ACCAACATTC CTTTTCCTTG CCCACATGCC GTATCAGTGA GTCTGTGAAG 
AAATTAATGG AACTCGCCTA TCAGACTTTA CTAGAGGCAA CAACCAGTAG TGATCAATGT 
GCTGTTCAAC TTTTCTACTC AGTGAGGAAT ATCTTCCATT TGTTCCATGA TGTTGTACCA 
ACATATCACA AGGAGAACCT TCAAAAACTT CCCCAGTTGG CTGCTATTCA TCACAACAAC 
TGTATGTACA TTGCTCACCA CTTGCTGACC CTCGGGCATC AGTTCAGATT GCGTCTTGCC 
CCCATTCTTT GTGATGGCAC TGCTACTTTT GTGGATCTTG TACCTGGCTT CAGGAGACTT 
GGGACAGAAT GCTTTTTGGC CCAAATGCGG GCACAGAAAG GTGAACTTCT GGAAAGATTA 
TCAAGTGCTA GGAACTTTTC AAATATGGAC GATGAAGAGA ATTATTCTGC AGCAAGTAAA 
GCAGTCCGGC AGGTACTGCA CCAACTAAAG AGACTTGGAA TTGTGTGGCA GGATGTCCTG 
CCAGTGAATA TATATTGCAA GGCTATGGGG ACTTTACTCA ATACAGCAAT TTCTGAGGTC 
ATTGGCAAAA TTACTGCCCT AGAGGACATA TCTACTGAAG ATGGTGATAG GTTATATTCC 
TTATGCAAAA CAGTGATGGA TGAAGGACCC CAAGTATTTG CACCTTTATC TGAAGAAAGC 
AAGAACAAGA AATATCAAGA AGAGGTTCCA GTCTATGTGC CAAAATGGAT GCCATTCAAG 
GAATTGATGA TGATGCTACA AGCCAGCTTG CAAGAAATTG GGGATCGGTG GGCAGATGGA 
AAAGGACCCC TGGCAGCTGC GTTCTCTTCC AGTGAAGTAA AAGCTTTAAT TCGTGCCTTG 
TTTCAGAACA CAGAAAGAAG AGCAGCTGCC CTTGCTAAAA TTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZW10 ( XM_017018558.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874047.1 Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X1, mRNA.

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CloneID	OHu92681
Clone ID Related Accession (Same CDS sequence)	XM_017018558.2 , XM_017018558.1
Accession Version	XM_017018558.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centromere/kinetochore protein zw10 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGATTTGCC ACTTGACACT GACCTGGAAA ATGAAAAAAC ATCTACTGTC 
CCATTGGCTG AGATGCTTGG AGACATGATC TGGGAGGACT TGTCTGAGTG CCTCATCAAA 
AACTGTTTGG TTTATTCGAT TCCAACAAAT AGCAGCAAAT TACAGCAATA TGAAGAGATC 
ATACAGTCCA CTGAAGAATT TGAAAATGCC CTAAAGGAAA TGAGATTTTT AAAAGGAGAT 
ACTACAGATT TGCTGAAATA CGCTCGTAAC ATCAATTCTC ATTTTGCAAA CAAAAAGTGC 
CAGGATGTGA TTGTGGCAGC CAGAAATCTA ATGACCTCAG AAATTCATAA CACTGTGAAG 
ATTATTCCTG ATTCTAAGAT AAATGTGCCA GAGTTACCCA CTCCTGATGA GGATAACAAA 
CTGGAAGTAC AGAAAGTATC CAATACTCAG TACCACGAAG TGATGAATTT AGAGCCTGAA 
AATACATTGG ACCAACATTC CTTTTCCTTG CCCACATGCC GTATCAGTGA GTCTGTGAAG 
AAATTAATGG AACTCGCCTA TCAGACTTTA CTAGAGGCAA CAACCAGTAG TGATCAATGT 
GCTGTTCAAC TTTTCTACTC AGTGAGGAAT ATCTTCCATT TGTTCCATGA TGTTGTACCA 
ACATATCACA AGGAGAACCT TCAAAAACTT CCCCAGTTGG CTGCTATTCA TCACAACAAC 
TGTATGTACA TTGCTCACCA CTTGCTGACC CTCGGGCATC AGTTCAGATT GCGTCTTGCC 
CCCATTCTTT GTGATGGCAC TGCTACTTTT GTGGATCTTG TACCTGGCTT CAGGAGACTT 
GGGACAGAAT GCTTTTTGGC CCAAATGCGG GCACAGAAAG GTGAACTTCT GGAAAGATTA 
TCAAGTGCTA GGAACTTTTC AAATATGGAC GATGAAGAGA ATTATTCTGC AGCAAGTAAA 
GCAGTCCGGC AGGTACTGCA CCAACTAAAG AGACTTGGAA TTGTGTGGCA GGATGTCCTG 
CCAGTGAATA TATATTGCAA GGCTATGGGG ACTTTACTCA ATACAGCAAT TTCTGAGGTC 
ATTGGCAAAA TTACTGCCCT AGAGGACATA TCTACTGAAG ATGGTGATAG GTTATATTCC 
TTATGCAAAA CAGTGATGGA TGAAGGACCC CAAGTATTTG CACCTTTATC TGAAGAAAGC 
AAGAACAAGA AATATCAAGA AGAGGTTCCA GTCTATGTGC CAAAATGGAT GCCATTCAAG 
GAATTGATGA TGATGCTACA AGCCAGCTTG CAAGAAATTG GGGATCGGTG GGCAGATGGA 
AAAGGACCCC TGGCAGCTGC GTTCTCTTCC AGTGAAGTAA AAGCTTTAAT TCGTGCCTTG 
TTTCAGAACA CAGAAAGAAG AGCAGCTGCC CTTGCTAAAA TTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874047.1
CDS	38..2185
Translation	MASFVTEVLAHSGRLEKEDLGTRISRLTRRVEEIKGEVCNMISKKYSEFLPSMQSAQGLITQVDKLSEDIDLLKSRIESEVRRDLHVSTGEFTDLKQQLERDSVVLSLLKQLQEFSTAIEEYNCALTEKKYVTGAQRLEEAQKCLKLLKSRKCFDLKILKSLSMELTIQKQNILYHLGEEWQKLIVWKFPPSKDTSSLESYLQTELHLYTEQSHKEEKTPMPPISSVLLAFSVLGELHSKLKSFDLPLDTDLENEKTSTVPLAEMLGDMIWEDLSECLIKNCLVYSIPTNSSKLQQYEEIIQSTEEFENALKEMRFLKGDTTDLLKYARNINSHFANKKCQDVIVAARNLMTSEIHNTVKIIPDSKINVPELPTPDEDNKLEVQKVSNTQYHEVMNLEPENTLDQHSFSLPTCRISESVKKLMELAYQTLLEATTSSDQCAVQLFYSVRNIFHLFHDVVPTYHKENLQKLPQLAAIHHNNCMYIAHHLLTLGHQFRLRLAPILCDGTATFVDLVPGFRRLGTECFLAQMRAQKGELLERLSSARNFSNMDDEENYSAASKAVRQVLHQLKRLGIVWQDVLPVNIYCKAMGTLLNTAISEVIGKITALEDISTEDGDRLYSLCKTVMDEGPQVFAPLSEESKNKKYQEEVPVYVPKWMPFKELMMMLQASLQEIGDRWADGKGPLAAAFSSSEVKALIRALFQNTERRAAALAKIK

Target ORF information:

RefSeq Version	XM_017018558.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zw10 kinetochore protein (ZW10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018558.1

ORF Insert Sequence:

ATGGCCTCGT TCGTGACAGA AGTTTTGGCA CACTCCGGGA GGCTGGAAAA GGAGGATCTG 
GGGACCCGGA TCAGCCGCCT GACCCGGCGG GTGGAGGAGA TCAAGGGTGA GGTGTGCAAT 
ATGATTAGCA AGAAGTACAG TGAATTCCTG CCTAGCATGC AGAGCGCGCA GGGCCTGATT 
ACCCAGGTGG ATAAGCTATC TGAAGACATT GACCTGCTGA AATCCAGGAT AGAGAGTGAG 
GTCCGCCGGG ATCTTCACGT ATCAACCGGT GAATTTACAG ACTTAAAGCA GCAGTTGGAA 
AGAGACTCAG TTGTCCTAAG TTTGCTTAAA CAGTTGCAGG AGTTTTCCAC TGCTATTGAA 
GAATATAATT GTGCATTAAC AGAGAAGAAG TATGTCACTG GTGCTCAGCG TCTGGAAGAG 
GCACAGAAAT GCTTGAAGTT ATTAAAATCC AGAAAATGCT TTGATTTAAA AATATTGAAA 
TCTCTCAGCA TGGAGCTCAC AATACAGAAA CAGAACATAC TTTATCACCT TGGAGAAGAG 
TGGCAGAAGC TGATTGTATG GAAGTTCCCA CCATCAAAAG ATACCAGCAG TTTGGAATCT 
TACCTACAAA CTGAACTTCA TTTATACACT GAACAATCGC ACAAAGAGGA GAAGACCCCT 
ATGCCACCCA TCAGTTCTGT CCTCTTGGCA TTTTCTGTTC TTGGAGAACT ACACAGCAAG 
CTTAAATCAT TTGATTTGCC ACTTGACACT GACCTGGAAA ATGAAAAAAC ATCTACTGTC 
CCATTGGCTG AGATGCTTGG AGACATGATC TGGGAGGACT TGTCTGAGTG CCTCATCAAA 
AACTGTTTGG TTTATTCGAT TCCAACAAAT AGCAGCAAAT TACAGCAATA TGAAGAGATC 
ATACAGTCCA CTGAAGAATT TGAAAATGCC CTAAAGGAAA TGAGATTTTT AAAAGGAGAT 
ACTACAGATT TGCTGAAATA CGCTCGTAAC ATCAATTCTC ATTTTGCAAA CAAAAAGTGC 
CAGGATGTGA TTGTGGCAGC CAGAAATCTA ATGACCTCAG AAATTCATAA CACTGTGAAG 
ATTATTCCTG ATTCTAAGAT AAATGTGCCA GAGTTACCCA CTCCTGATGA GGATAACAAA 
CTGGAAGTAC AGAAAGTATC CAATACTCAG TACCACGAAG TGATGAATTT AGAGCCTGAA 
AATACATTGG ACCAACATTC CTTTTCCTTG CCCACATGCC GTATCAGTGA GTCTGTGAAG 
AAATTAATGG AACTCGCCTA TCAGACTTTA CTAGAGGCAA CAACCAGTAG TGATCAATGT 
GCTGTTCAAC TTTTCTACTC AGTGAGGAAT ATCTTCCATT TGTTCCATGA TGTTGTACCA 
ACATATCACA AGGAGAACCT TCAAAAACTT CCCCAGTTGG CTGCTATTCA TCACAACAAC 
TGTATGTACA TTGCTCACCA CTTGCTGACC CTCGGGCATC AGTTCAGATT GCGTCTTGCC 
CCCATTCTTT GTGATGGCAC TGCTACTTTT GTGGATCTTG TACCTGGCTT CAGGAGACTT 
GGGACAGAAT GCTTTTTGGC CCAAATGCGG GCACAGAAAG GTGAACTTCT GGAAAGATTA 
TCAAGTGCTA GGAACTTTTC AAATATGGAC GATGAAGAGA ATTATTCTGC AGCAAGTAAA 
GCAGTCCGGC AGGTACTGCA CCAACTAAAG AGACTTGGAA TTGTGTGGCA GGATGTCCTG 
CCAGTGAATA TATATTGCAA GGCTATGGGG ACTTTACTCA ATACAGCAAT TTCTGAGGTC 
ATTGGCAAAA TTACTGCCCT AGAGGACATA TCTACTGAAG ATGGTGATAG GTTATATTCC 
TTATGCAAAA CAGTGATGGA TGAAGGACCC CAAGTATTTG CACCTTTATC TGAAGAAAGC 
AAGAACAAGA AATATCAAGA AGAGGTTCCA GTCTATGTGC CAAAATGGAT GCCATTCAAG 
GAATTGATGA TGATGCTACA AGCCAGCTTG CAAGAAATTG GGGATCGGTG GGCAGATGGA 
AAAGGACCCC TGGCAGCTGC GTTCTCTTCC AGTGAAGTAA AAGCTTTAAT TCGTGCCTTG 
TTTCAGAACA CAGAAAGAAG AGCAGCTGCC CTTGCTAAAA TTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex.
Acta crystallographica. Section F, Structural biology communications71(Pt 4)438-42(2015 Apr)
Altenfeld A,Wohlgemuth S,Wehenkel A,Vetter IR,Musacchio A

PtdIns(3)P-bound UVRAG coordinates Golgi-ER retrograde and Atg9 transport by differential interactions with the ER tether and the beclin?1 complex.
Nature cell biology15(10)1206-1219(2013 Oct)
He S,Ni D,Ma B,Lee JH,Zhang T,Ghozalli I,Pirooz SD,Zhao Z,Bharatham N,Li B,Oh S,Lee WH,Takahashi Y,Wang HG,Minassian A,Feng P,Deretic V,Pepperkok R,Tagaya M,Yoon HS,Liang C

hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain.
The Biochemical journal436(1)157-68(2011 May)
Vos LJ,Famulski JK,Chan GK

Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery.
Structure (London, England : 1993)18(5)616-26(2010 May)
Civril F,Wehenkel A,Giorgi FM,Santaguida S,Di Fonzo A,Grigorean G,Ciccarelli FD,Musacchio A

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ZW10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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