Irx3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irx3
Entrez Gene ID	307721
Full Name	iroquois homeobox 3
General protein information	Preferred Names iroquois homeobox 3 Names iroquois-class homeodomain protein IRX-3 Iroquois related homeobox 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255191.2	XP_006255253.1	iroquois-class homeodomain protein IRX-3 isoform X1
NM_001107413.1	NP_001100883.1	iroquois-class homeodomain protein IRX-3
XM_006255192.3	XP_006255254.1	iroquois-class homeodomain protein IRX-3 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Nutrition modulates Fto and Irx3 gene transcript levels, but does not alter their DNA methylation profiles in rat white adipose tissues.
Nowacka-Woszuk J, Pruszynska-Oszmalek E, Szydlowski M, Szczerbal I
Gene61044-48(2017 Apr)

Obesity-associated variants within FTO form long-range functional connections with IRX3.
Smemo S, Tena JJ, Kim KH, Gamazon ER, Sakabe NJ, G?mez-Mar?n C, Aneas I, Credidio FL, Sobreira DR, Wasserman NF, Lee JH, Puviindran V, Tam D, Shen M, Son JE, Vakili NA, Sung HK, Naranjo S, Acemel RD, Manzanares M, Nagy A, Cox NJ, Hui CC, Gomez-Skarmeta JL, N?brega MA
Nature507(7492)371-5(2014 Mar)

Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity.
Song MR, Sun Y, Bryson A, Gill GN, Evans SM, Pfaff SL
Development (Cambridge, England)136(17)2923-32(2009 Sep)

The prepattern transcription factor Irx3 directs nephron segment identity.
Reggiani L, Raciti D, Airik R, Kispert A, Br?ndli AW
Genes & development21(18)2358-70(2007 Sep)

Biological activities encoded by the murine mesenchymal stem cell transcriptome provide a basis for their developmental potential and broad therapeutic efficacy.
Phinney DG, Hill K, Michelson C, DuTreil M, Hughes C, Humphries S, Wilkinson R, Baddoo M, Bayly E
Stem cells (Dayton, Ohio)24(1)186-98(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study revealed that diet was an important factor modulating transcription of Fto and Irx3, but its effect is time-dependent. In contrast, the DNA methylation profiles of Fto and Irx3 were not altered by nutrition, which may indicate that the feeding type, when applied postnatally, did not affect DNA methylation of these genes.
Title: Nutrition modulates Fto and Irx3 gene transcript levels, but does not alter their DNA methylation profiles in rat white adipose tissues.

The following Irx3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irx3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03454	NM_001107413.1 Latest version!	Rattus norvegicus iroquois homeobox 3 (Irx3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa38657	XM_006255191.2 Latest version!	Rattus norvegicus iroquois homeobox 3 (Irx3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa38658	XM_006255192.3 Latest version!	Rattus norvegicus iroquois homeobox 3 (Irx3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irx3 ( NM_001107413.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100883.1 Rattus norvegicus iroquois homeobox 3 (Irx3), mRNA.

Price & Availability↑

CloneID	ORa03454
Clone ID Related Accession (Same CDS sequence)	NM_001107413.1
Accession Version	NM_001107413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	iroquois-class homeodomain protein IRX-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474037.2. On or before Oct 4, 2007 this sequence version replaced XM_001061997.1, XM_226322.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTTGGCCA TCATCACCAA AATGACCCTC ACCCAGGTGT CCACCTGGTT CGCCAACGCG 
CGCCGGCGCC TCAAGAAGGA AAACAAGATG ACGTGGGCTC CCCGTAGTCG CACGGACGAG 
GAGGGCAATG CTTATGGGAG CGAGCGGGAG GAGGAGGACG AAGAGGAAGA CGAGGAAGAG 
GGCAAACGCG AGCTGGAGAT GGAGGAGGAG GAGCTCGCAG GAGAGGAGGA GGACACGGGG 
GGCGAGGGCC TGGCCGACGA CGACGAGGAT GAGGAGATCG ATTTGGAAAA CTTAGACAGC 
GCGGCAGCCG GGTCGGAACT AACCCTGGCT GGGACGGCGC ATAGGAACGG AGACTTCGGC 
CTAGGACCCA TTTCAGACTG CAAAAATAGC GACTCGGACG ACAGCTCCGA AAGCTTGGAG 
GAGCGACCAC TGTCCGTCCT GAGCTTGGCT CCCGCGCCAC CGCCCGTGGC CAGGGCTCCT 
GCGTCTCCAC CTTCTCCACC CTCCGGCCTG GACCCCTGCG CTCCGGCACC GGCGCCCTCC 
TCCACCCTCC AGAAGCCCAA GATCTGGTCA CTGGCCGAGA CGGCCACTAG CCCGGACAAC 
CCACGTCGAT CGCCTCCCGG AGCCGGAGGT TCTCCTCCCG GGGCAGCCGT TGCGCCCCCG 
ACGCTGCAGC TCTCGCCCGC GGCCGCCGCA GCGGCTGCTG CAGCACACAG ACTGGTGTCG 
GCGCCGCTTG GCAAATTCCC CGCTTGGACC AACAGGCCTT TCCCAGGCCC GCCGGCCGGT 
CCGCGGCCGC ACCCGCTGTC CATGCTAGGC TCGGCCCCAC AGCACCTGCT GGGACTTCCC 
GGACCAGAGA GTGGAACAGA TCGCTGTAGT GCCTTGGAAG TGGAGAAAAA GTTACTTAAG 
ACAGCTTTCC AGCCGGTGCC AAGGCGGCCC CAGAACCACC TGGATGCTGC TCTGGTCTTA 
TCAGCTCTCT CTTCGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100883.1
CDS	217..1197
Translation	MLAIITKMTLTQVSTWFANARRRLKKENKMTWAPRSRTDEEGNAYGSEREEEDEEEDEEEGKRELEMEEEELAGEEEDTGGEGLADDDEDEEIDLENLDSAAAGSELTLAGTAHRNGDFGLGPISDCKNSDSDDSSESLEERPLSVLSLAPAPPPVARAPASPPSPPSGLDPCAPAPAPSSTLQKPKIWSLAETATSPDNPRRSPPGAGGSPPGAAVAPPTLQLSPAAAAAAAAAHRLVSAPLGKFPAWTNRPFPGPPAGPRPHPLSMLGSAPQHLLGLPGPESGTDRCSALEVEKKLLKTAFQPVPRRPQNHLDAALVLSALSSS

Target ORF information:

RefSeq Version	NM_001107413.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iroquois homeobox 3 (Irx3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107413.1

ORF Insert Sequence:

ATGTTGGCCA TCATCACCAA AATGACCCTC ACCCAGGTGT CCACCTGGTT CGCCAACGCG 
CGCCGGCGCC TCAAGAAGGA AAACAAGATG ACGTGGGCTC CCCGTAGTCG CACGGACGAG 
GAGGGCAATG CTTATGGGAG CGAGCGGGAG GAGGAGGACG AAGAGGAAGA CGAGGAAGAG 
GGCAAACGCG AGCTGGAGAT GGAGGAGGAG GAGCTCGCAG GAGAGGAGGA GGACACGGGG 
GGCGAGGGCC TGGCCGACGA CGACGAGGAT GAGGAGATCG ATTTGGAAAA CTTAGACAGC 
GCGGCAGCCG GGTCGGAACT AACCCTGGCT GGGACGGCGC ATAGGAACGG AGACTTCGGC 
CTAGGACCCA TTTCAGACTG CAAAAATAGC GACTCGGACG ACAGCTCCGA AAGCTTGGAG 
GAGCGACCAC TGTCCGTCCT GAGCTTGGCT CCCGCGCCAC CGCCCGTGGC CAGGGCTCCT 
GCGTCTCCAC CTTCTCCACC CTCCGGCCTG GACCCCTGCG CTCCGGCACC GGCGCCCTCC 
TCCACCCTCC AGAAGCCCAA GATCTGGTCA CTGGCCGAGA CGGCCACTAG CCCGGACAAC 
CCACGTCGAT CGCCTCCCGG AGCCGGAGGT TCTCCTCCCG GGGCAGCCGT TGCGCCCCCG 
ACGCTGCAGC TCTCGCCCGC GGCCGCCGCA GCGGCTGCTG CAGCACACAG ACTGGTGTCG 
GCGCCGCTTG GCAAATTCCC CGCTTGGACC AACAGGCCTT TCCCAGGCCC GCCGGCCGGT 
CCGCGGCCGC ACCCGCTGTC CATGCTAGGC TCGGCCCCAC AGCACCTGCT GGGACTTCCC 
GGACCAGAGA GTGGAACAGA TCGCTGTAGT GCCTTGGAAG TGGAGAAAAA GTTACTTAAG 
ACAGCTTTCC AGCCGGTGCC AAGGCGGCCC CAGAACCACC TGGATGCTGC TCTGGTCTTA 
TCAGCTCTCT CTTCGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irx3 ( XM_006255191.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255253.1 Rattus norvegicus iroquois homeobox 3 (Irx3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38657
Clone ID Related Accession (Same CDS sequence)	XM_006255191.2
Accession Version	XM_006255191.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	iroquois-class homeodomain protein IRX-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCC CCCAGCTCGG ATACCAGTAC ATCCGCCCGC TCTACCCACC CGAGCGCCCG 
CCAGCCGCGG GCGGCGGCGG AGGTGGCAGT AGCGCTGGGG GCCGGAGCGG TCCGGGTGCC 
GGAGCCTCCG AGTTGGCCGC CTCGGGGTCC CTATCCAATG TGCTTTCGTC CGTGTACGGG 
GCACCCTACG CCGCGGCCGC AGCAGCCGCC GCCGCCGCCC AGGGGTACGG CGCCTTCCTG 
CCCTACGCCA CGGAGCTGCC CATCTTCCCA CAGCTGGGCG CTCAGTATGA GCTGAAGGAC 
AGCCCTGGGG TCCAGCATCC GGCCACGGCC GCCGCGTTCC CGCACCCGCA CCCCGCCTTC 
TACCCCTACG GCCAGTACCA GTTCGGGGAC CCGTCCCGTC CCAAGAACGC CACCCGGGAA 
AGCACAAGCA CGCTTAAGGC CTGGCTCAAT GAACACCGCA AGAACCCGTA CCCCACCAAG 
GGCGAGAAGA TCATGTTGGC CATCATCACC AAAATGACCC TCACCCAGGT GTCCACCTGG 
TTCGCCAACG CGCGCCGGCG CCTCAAGAAG GAAAACAAGA TGACGTGGGC TCCCCGTAGT 
CGCACGGACG AGGAGGGCAA TGCTTATGGG AGCGAGCGGG AGGAGGAGGA CGAAGAGGAA 
GACGAGGAAG AGGGCAAACG CGAGCTGGAG ATGGAGGAGG AGGAGCTCGC AGGAGAGGAG 
GAGGACACGG GGGGCGAGGG CCTGGCCGAC GACGACGAGG ATGAGGAGAT CGATTTGGAA 
AACTTAGACA GCGCGGCAGC CGGGTCGGAA CTAACCCTGG CTGGGACGGC GCATAGGAAC 
GGAGACTTCG GCCTAGGACC CATTTCAGAC TGCAAAAATA GCGACTCGGA CGACAGCTCC 
GAAAGCTTGG AGGAGCGACC ACTGTCCGTC CTGAGCTTGG CTCCCGCGCC ACCGCCCGTG 
GCCAGGGCTC CTGCGTCTCC ACCTTCTCCA CCCTCCGGCC TGGACCCCTG CGCTCCGGCA 
CCGGCGCCCT CCTCCACCCT CCAGAAGCCC AAGATCTGGT CACTGGCCGA GACGGCCACT 
AGCCCGGACA ACCCACGTCG ATCGCCTCCC GGAGCCGGAG GTTCTCCTCC CGGGGCAGCC 
GTTGCGCCCC CGACGCTGCA GCTCTCGCCC GCGGCCGCCG CAGCGGCTGC TGCAGCACAC 
AGACTGGTGT CGGCGCCGCT TGGCAAATTC CCCGCTTGGA CCAACAGGCC TTTCCCAGGC 
CCGCCGGCCG GTCCGCGGCC GCACCCGCTG TCCATGCTAG GCTCGGCCCC ACAGCACCTG 
CTGGGACTTC CCGGAGCCGC AGGCCACCCC GCTGCGGCCG CCGCCGCCTA TGCTCGGCCT 
GCGGAGCCAG AGAGTGGAAC AGATCGCTGT AGTGCCTTGG AAGTGGAGAA AAAGTTACTT 
AAGACAGCTT TCCAGCCGGT GCCAAGGCGG CCCCAGAACC ACCTGGATGC TGCTCTGGTC 
TTATCAGCTC TCTCTTCGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255253.1
CDS	704..2227
Translation	MSFPQLGYQYIRPLYPPERPPAAGGGGGGSSAGGRSGPGAGASELAASGSLSNVLSSVYGAPYAAAAAAAAAAQGYGAFLPYATELPIFPQLGAQYELKDSPGVQHPATAAAFPHPHPAFYPYGQYQFGDPSRPKNATRESTSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWAPRSRTDEEGNAYGSEREEEDEEEDEEEGKRELEMEEEELAGEEEDTGGEGLADDDEDEEIDLENLDSAAAGSELTLAGTAHRNGDFGLGPISDCKNSDSDDSSESLEERPLSVLSLAPAPPPVARAPASPPSPPSGLDPCAPAPAPSSTLQKPKIWSLAETATSPDNPRRSPPGAGGSPPGAAVAPPTLQLSPAAAAAAAAAHRLVSAPLGKFPAWTNRPFPGPPAGPRPHPLSMLGSAPQHLLGLPGAAGHPAAAAAAYARPAEPESGTDRCSALEVEKKLLKTAFQPVPRRPQNHLDAALVLSALSSS

Target ORF information:

RefSeq Version	XM_006255191.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iroquois homeobox 3 (Irx3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255191.2

ORF Insert Sequence:

ATGTCCTTCC CCCAGCTCGG ATACCAGTAC ATCCGCCCGC TCTACCCACC CGAGCGCCCG 
CCAGCCGCGG GCGGCGGCGG AGGTGGCAGT AGCGCTGGGG GCCGGAGCGG TCCGGGTGCC 
GGAGCCTCCG AGTTGGCCGC CTCGGGGTCC CTATCCAATG TGCTTTCGTC CGTGTACGGG 
GCACCCTACG CCGCGGCCGC AGCAGCCGCC GCCGCCGCCC AGGGGTACGG CGCCTTCCTG 
CCCTACGCCA CGGAGCTGCC CATCTTCCCA CAGCTGGGCG CTCAGTATGA GCTGAAGGAC 
AGCCCTGGGG TCCAGCATCC GGCCACGGCC GCCGCGTTCC CGCACCCGCA CCCCGCCTTC 
TACCCCTACG GCCAGTACCA GTTCGGGGAC CCGTCCCGTC CCAAGAACGC CACCCGGGAA 
AGCACAAGCA CGCTTAAGGC CTGGCTCAAT GAACACCGCA AGAACCCGTA CCCCACCAAG 
GGCGAGAAGA TCATGTTGGC CATCATCACC AAAATGACCC TCACCCAGGT GTCCACCTGG 
TTCGCCAACG CGCGCCGGCG CCTCAAGAAG GAAAACAAGA TGACGTGGGC TCCCCGTAGT 
CGCACGGACG AGGAGGGCAA TGCTTATGGG AGCGAGCGGG AGGAGGAGGA CGAAGAGGAA 
GACGAGGAAG AGGGCAAACG CGAGCTGGAG ATGGAGGAGG AGGAGCTCGC AGGAGAGGAG 
GAGGACACGG GGGGCGAGGG CCTGGCCGAC GACGACGAGG ATGAGGAGAT CGATTTGGAA 
AACTTAGACA GCGCGGCAGC CGGGTCGGAA CTAACCCTGG CTGGGACGGC GCATAGGAAC 
GGAGACTTCG GCCTAGGACC CATTTCAGAC TGCAAAAATA GCGACTCGGA CGACAGCTCC 
GAAAGCTTGG AGGAGCGACC ACTGTCCGTC CTGAGCTTGG CTCCCGCGCC ACCGCCCGTG 
GCCAGGGCTC CTGCGTCTCC ACCTTCTCCA CCCTCCGGCC TGGACCCCTG CGCTCCGGCA 
CCGGCGCCCT CCTCCACCCT CCAGAAGCCC AAGATCTGGT CACTGGCCGA GACGGCCACT 
AGCCCGGACA ACCCACGTCG ATCGCCTCCC GGAGCCGGAG GTTCTCCTCC CGGGGCAGCC 
GTTGCGCCCC CGACGCTGCA GCTCTCGCCC GCGGCCGCCG CAGCGGCTGC TGCAGCACAC 
AGACTGGTGT CGGCGCCGCT TGGCAAATTC CCCGCTTGGA CCAACAGGCC TTTCCCAGGC 
CCGCCGGCCG GTCCGCGGCC GCACCCGCTG TCCATGCTAG GCTCGGCCCC ACAGCACCTG 
CTGGGACTTC CCGGAGCCGC AGGCCACCCC GCTGCGGCCG CCGCCGCCTA TGCTCGGCCT 
GCGGAGCCAG AGAGTGGAAC AGATCGCTGT AGTGCCTTGG AAGTGGAGAA AAAGTTACTT 
AAGACAGCTT TCCAGCCGGT GCCAAGGCGG CCCCAGAACC ACCTGGATGC TGCTCTGGTC 
TTATCAGCTC TCTCTTCGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irx3 ( XM_006255192.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255254.1 Rattus norvegicus iroquois homeobox 3 (Irx3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38658
Clone ID Related Accession (Same CDS sequence)	XM_006255192.3
Accession Version	XM_006255192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	iroquois-class homeodomain protein IRX-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCC CCCAGCTCGG ATACCAGTAC ATCCGCCCGC TCTACCCACC CGAGCGCCCG 
CCAGCCGCGG GCGGCGGCGG AGGTGGCAGT AGCGCTGGGG GCCGGAGCGG TCCGGGTGCC 
GGAGCCTCCG AGTTGGCCGC CTCGGGGTCC CTATCCAATG TGCTTTCGTC CGTGTACGGG 
GCACCCTACG CCGCGGCCGC AGCAGCCGCC GCCGCCGCCC AGGGGTACGG CGCCTTCCTG 
CCCTACGCCA CGGAGCTGCC CATCTTCCCA CAGCTGGGCG CTCAGTATGA GCTGAAGGAC 
AGCCCTGGGG TCCAGCATCC GGCCACGGCC GCCGCGTTCC CGCACCCGCA CCCCGCCTTC 
TACCCCTACG GCCAGTACCA GTTCGGGGAC CCGTCCCGTC CCAAGAACGC CACCCGGGAA 
AGCACAAGCA CGCTTAAGGC CTGGCTCAAT GAACACCGCA AGAACCCGTA CCCCACCAAG 
GGCGAGAAGA TCATGTTGGC CATCATCACC AAAATGACCC TCACCCAGGT GTCCACCTGG 
TTCGCCAACG CGCGCCGGCG CCTCAAGAAG GAAAACAAGA TGACGTGGGC TCCCCGTAGT 
CGCACGGACG AGGAGGGCAA TGCTTATGGG AGCGAGCGGG AGGAGGAGGA CGAAGAGGAA 
GACGAGGAAG AGGGCAAACG CGAGCTGGAG ATGGAGGAGG AGGAGCTCGC AGGAGAGGAG 
GAGGACACGG GGGGCGAGGG CCTGGCCGAC GACGACGAGG ATGAGGAGAT CGATTTGGAA 
AACTTAGACA GCGCGGCAGC CGGGTCGGAA CTAACCCTGG CTGGGACGGC GCATAGGAAC 
GGAGACTTCG GCCTAGGACC CATTTCAGAC TGCAAAAATA GCGACTCGGA CGACAGCTCC 
GAAAGCTTGG AGGAGCGACC ACTGTCCGTC CTGAGCTTGG CTCCCGCGCC ACCGCCCGTG 
GCCAGGGCTC CTGCGTCTCC ACCTTCTCCA CCCTCCGGCC TGGACCCCTG CGCTCCGGCA 
CCGGCGCCCT CCTCCACCCT CCAGAAGCCC AAGATCTGGT CACTGGCCGA GACGGCCACT 
AGCCCGGACA ACCCACGTCG ATCGCCTCCC GGAGCCGGAG GTTCTCCTCC CGGGGCAGCC 
GTTGCGCCCC CGACGCTGCA GCTCTCGCCC GCGGCCGCCG CAGCGGCTGC TGCAGCACAC 
AGACTGGTGT CGGCGCCGCT TGGCAAATTC CCCGCTTGGA CCAACAGGCC TTTCCCAGGC 
CCGCCGGCCG GTCCGCGGCC GCACCCGCTG TCCATGCTAG GCTCGGCCCC ACAGCACCTG 
CTGGGACTTC CCGGAGCCGC AGGCCACCCC GCTGCGGCCG CCGCCGCCTA TGCTCGGCCT 
GCGGAGCCAG AGAGTGGAAC AGGTGACAAG AAAAATATCG CTGTAGTGCC TTGGAAGTGG 
AGAAAAAGTT ACTTAAGACA GCTTTCCAGC CGGTGCCAAG GCGGCCCCAG AACCACCTGG 
ATGCTGCTCT GGTCTTATCA GCTCTCTCTT CGTCTTAACT TTTCAACAAT TTTTAAATCG 
TTGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255254.1
CDS	704..2272
Translation	MSFPQLGYQYIRPLYPPERPPAAGGGGGGSSAGGRSGPGAGASELAASGSLSNVLSSVYGAPYAAAAAAAAAAQGYGAFLPYATELPIFPQLGAQYELKDSPGVQHPATAAAFPHPHPAFYPYGQYQFGDPSRPKNATRESTSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWAPRSRTDEEGNAYGSEREEEDEEEDEEEGKRELEMEEEELAGEEEDTGGEGLADDDEDEEIDLENLDSAAAGSELTLAGTAHRNGDFGLGPISDCKNSDSDDSSESLEERPLSVLSLAPAPPPVARAPASPPSPPSGLDPCAPAPAPSSTLQKPKIWSLAETATSPDNPRRSPPGAGGSPPGAAVAPPTLQLSPAAAAAAAAAHRLVSAPLGKFPAWTNRPFPGPPAGPRPHPLSMLGSAPQHLLGLPGAAGHPAAAAAAYARPAEPESGTGDKKNIAVVPWKWRKSYLRQLSSRCQGGPRTTWMLLWSYQLSLRLNFSTIFKSLY

Target ORF information:

RefSeq Version	XM_006255192.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iroquois homeobox 3 (Irx3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255192.3

ORF Insert Sequence:

ATGTCCTTCC CCCAGCTCGG ATACCAGTAC ATCCGCCCGC TCTACCCACC CGAGCGCCCG 
CCAGCCGCGG GCGGCGGCGG AGGTGGCAGT AGCGCTGGGG GCCGGAGCGG TCCGGGTGCC 
GGAGCCTCCG AGTTGGCCGC CTCGGGGTCC CTATCCAATG TGCTTTCGTC CGTGTACGGG 
GCACCCTACG CCGCGGCCGC AGCAGCCGCC GCCGCCGCCC AGGGGTACGG CGCCTTCCTG 
CCCTACGCCA CGGAGCTGCC CATCTTCCCA CAGCTGGGCG CTCAGTATGA GCTGAAGGAC 
AGCCCTGGGG TCCAGCATCC GGCCACGGCC GCCGCGTTCC CGCACCCGCA CCCCGCCTTC 
TACCCCTACG GCCAGTACCA GTTCGGGGAC CCGTCCCGTC CCAAGAACGC CACCCGGGAA 
AGCACAAGCA CGCTTAAGGC CTGGCTCAAT GAACACCGCA AGAACCCGTA CCCCACCAAG 
GGCGAGAAGA TCATGTTGGC CATCATCACC AAAATGACCC TCACCCAGGT GTCCACCTGG 
TTCGCCAACG CGCGCCGGCG CCTCAAGAAG GAAAACAAGA TGACGTGGGC TCCCCGTAGT 
CGCACGGACG AGGAGGGCAA TGCTTATGGG AGCGAGCGGG AGGAGGAGGA CGAAGAGGAA 
GACGAGGAAG AGGGCAAACG CGAGCTGGAG ATGGAGGAGG AGGAGCTCGC AGGAGAGGAG 
GAGGACACGG GGGGCGAGGG CCTGGCCGAC GACGACGAGG ATGAGGAGAT CGATTTGGAA 
AACTTAGACA GCGCGGCAGC CGGGTCGGAA CTAACCCTGG CTGGGACGGC GCATAGGAAC 
GGAGACTTCG GCCTAGGACC CATTTCAGAC TGCAAAAATA GCGACTCGGA CGACAGCTCC 
GAAAGCTTGG AGGAGCGACC ACTGTCCGTC CTGAGCTTGG CTCCCGCGCC ACCGCCCGTG 
GCCAGGGCTC CTGCGTCTCC ACCTTCTCCA CCCTCCGGCC TGGACCCCTG CGCTCCGGCA 
CCGGCGCCCT CCTCCACCCT CCAGAAGCCC AAGATCTGGT CACTGGCCGA GACGGCCACT 
AGCCCGGACA ACCCACGTCG ATCGCCTCCC GGAGCCGGAG GTTCTCCTCC CGGGGCAGCC 
GTTGCGCCCC CGACGCTGCA GCTCTCGCCC GCGGCCGCCG CAGCGGCTGC TGCAGCACAC 
AGACTGGTGT CGGCGCCGCT TGGCAAATTC CCCGCTTGGA CCAACAGGCC TTTCCCAGGC 
CCGCCGGCCG GTCCGCGGCC GCACCCGCTG TCCATGCTAG GCTCGGCCCC ACAGCACCTG 
CTGGGACTTC CCGGAGCCGC AGGCCACCCC GCTGCGGCCG CCGCCGCCTA TGCTCGGCCT 
GCGGAGCCAG AGAGTGGAAC AGGTGACAAG AAAAATATCG CTGTAGTGCC TTGGAAGTGG 
AGAAAAAGTT ACTTAAGACA GCTTTCCAGC CGGTGCCAAG GCGGCCCCAG AACCACCTGG 
ATGCTGCTCT GGTCTTATCA GCTCTCTCTT CGTCTTAACT TTTCAACAAT TTTTAAATCG 
TTGTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nutrition modulates Fto and Irx3 gene transcript levels, but does not alter their DNA methylation profiles in rat white adipose tissues.
Gene61044-48(2017 Apr)
Nowacka-Woszuk J,Pruszynska-Oszmalek E,Szydlowski M,Szczerbal I

Obesity-associated variants within FTO form long-range functional connections with IRX3.
Nature507(7492)371-5(2014 Mar)
Smemo S,Tena JJ,Kim KH,Gamazon ER,Sakabe NJ,G?mez-Mar?n C,Aneas I,Credidio FL,Sobreira DR,Wasserman NF,Lee JH,Puviindran V,Tam D,Shen M,Son JE,Vakili NA,Sung HK,Naranjo S,Acemel RD,Manzanares M,Nagy A,Cox NJ,Hui CC,Gomez-Skarmeta JL,N?brega MA

Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity.
Development (Cambridge, England)136(17)2923-32(2009 Sep)
Song MR,Sun Y,Bryson A,Gill GN,Evans SM,Pfaff SL

The prepattern transcription factor Irx3 directs nephron segment identity.
Genes & development21(18)2358-70(2007 Sep)
Reggiani L,Raciti D,Airik R,Kispert A,Br?ndli AW

Biological activities encoded by the murine mesenchymal stem cell transcriptome provide a basis for their developmental potential and broad therapeutic efficacy.
Stem cells (Dayton, Ohio)24(1)186-98(2006 Jan)
Phinney DG,Hill K,Michelson C,DuTreil M,Hughes C,Humphries S,Wilkinson R,Baddoo M,Bayly E

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Irx3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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