Tlr6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tlr6
Entrez Gene ID	305353
Full Name	toll-like receptor 6
General protein information	Preferred Names toll-like receptor 6 Names toll-like receptor 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11
Summary	member of the Toll-like receptor (TLR, for whom the bell...) family which plays a role in pathogen recognition and activation of the immune response; interacts with Tlr2 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_207604.1	NP_997487.1	toll-like receptor 6
XM_006250960.2	XP_006251022.1	toll-like receptor 6 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLR6	NP_006059.2
Gallus gallus (chicken)	TLR1LA	NP_001007489.4
Rattus norvegicus (Norway rat)	Tlr6	NP_997487.1
Xenopus tropicalis (tropical clawed frog)	LOC100493946	XP_004910987.1
	TLR6	NP_001123902.1
Mus musculus (house mouse)	Tlr6	NP_035734.3
Pan troglodytes (chimpanzee)	TLR6	NP_001123940.1
Canis lupus familiaris (dog)	TLR6	XP_005618690.1
Bos taurus (cattle)	TLR6	NP_001001159.1
Gallus gallus (chicken)	TLR1LB	NP_001075178.3

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure of TIR domain of TLR6 reveals novel dimeric interface of TIR-TIR interaction for toll-like receptor signaling pathway.
Jang TH, Park HH
Journal of molecular biology426(19)3305-3313(2014 Sep)

Regulation of angiogenesis, mural cell recruitment and adventitial macrophage behavior by Toll-like receptors.
Aplin AC, Ligresti G, Fogel E, Zorzi P, Smith K, Nicosia RF
Angiogenesis17(1)147-61(2014 Jan)

CD36 coordinates NLRP3 inflammasome activation by facilitating intracellular nucleation of soluble ligands into particulate ligands in sterile inflammation.
Sheedy FJ, Grebe A, Rayner KJ, Kalantari P, Ramkhelawon B, Carpenter SB, Becker CE, Ediriweera HN, Mullick AE, Golenbock DT, Stuart LM, Latz E, Fitzgerald KA, Moore KJ
Nature immunology14(8)812-20(2013 Aug)

TLR2 is a primary receptor for Alzheimer's amyloid ? peptide to trigger neuroinflammatory activation.
Liu S, Liu Y, Hao W, Wolf L, Kiliaan AJ, Penke B, R?be CE, Walter J, Heneka MT, Hartmann T, Menger MD, Fassbender K
Journal of immunology (Baltimore, Md. : 1950)188(3)1098-107(2012 Feb)

Isolated Toll-like receptor transmembrane domains are capable of oligomerization.
Godfroy JI 3rd, Roostan M, Moroz YS, Korendovych IV, Yin H
PloS one7(11)e48875(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

aortic angiogenesis is preceded by an immune reaction with overexpression of Toll-like receptors (TLRs) and TLR-inducible genes.
Title: Regulation of angiogenesis, mural cell recruitment and adventitial macrophage behavior by Toll-like receptors.

toll-like receptors and nucleotide-binding oligomerization domain 2 combinations are contributors to the signaling cascades resulting in purinergic microglial activation
Title: Combinations of TLR and NOD2 ligands stimulate rat microglial P2X4R expression.

activation of TLR-2 and -6 can induce systemic inflammation in rats accompanied by the classical signs of brain-controlled illness responses
Title: Pyrexia, anorexia, adipsia, and depressed motor activity in rats during systemic inflammation induced by the Toll-like receptors-2 and -6 agonists MALP-2 and FSL-1.

The following Tlr6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tlr6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42242	NM_207604.1 Latest version!	Rattus norvegicus toll-like receptor 6 (Tlr6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa36070	XM_006250960.2 Latest version!	Rattus norvegicus toll-like receptor 6 (Tlr6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tlr6 ( NM_207604.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997487.1 Rattus norvegicus toll-like receptor 6 (Tlr6), mRNA.

Price & Availability↑

CloneID	ORa42242
Clone ID Related Accession (Same CDS sequence)	NM_207604.1
Accession Version	NM_207604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	toll-like receptor 6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062390.1. On Aug 30, 2004 this sequence version replaced XM_223420.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062390.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTAAAAT CCCTCTGGGA TAGCCTCTGC AACATGTCCC AAGACAGAGA ACCCATCGTG 
GAGAGTTTCC ATTTTGTTTG CACCCTGGCC TTAATAGTCG GAAGCATGAC CCAGTTCTCT 
GATGAACTTG AGTCTGTAGT AGACTATTCA AACAAGAACC TTACTCATGT CCCAAAAGAC 
CTGTCACCAA GCACAAAATC CTTGAGTCTG TCTCAAAACT CCATATCTGA TCTTCAGATG 
TCTGATATCA GCTTTCTGTC AGAGCTGAGA GTTCTGAGAC TCTCCCACAA CAGAATACGG 
AGACTTGACT TTGGTGTGTT CTTGCTCAAT CGGGACTTAG AATACCTGGA TGTCTCTCAC 
AATCAGTTAC AAAACATCTC TTGCTGTCCT ATGGTGAACT TGAAACATCT AGACCTCTCA 
TTCAATGATT TTGAAGTGCT GCCCGTGTAT AAGGAATTTG GCAACTTGAG GAAGCTGAGT 
TTCTTGGGAT TAAGTGCTGC AAAGTTCCGA CAACTGGATC TGCTCCCAAT TTCTCACTTG 
CACCTGAGCT GTGTTCTTCT GGACTTGGTG AATTATCAGA TAAAAGATGG TGAAACAGAA 
AGTCTTCAGG TTCCAAATAC CAACGTTCTC CATTTGGTCT TTCATCCGAA TAGCCTGTTC 
TCTGTGCAAG TGAACATATC TGTAAATGCT TTAGGATGCT TACAACTGAG TAATATTAAA 
TTGAATGATG AAAACTGTCA AAGCTTAATT ATATTTTTAT CAGAACTCAC CAGAGGTCCA 
ACCTTATTGA ATCTGACCCT CCAGCACATA GAAACAAACT GGAAGTGCTT TGTTAGACTT 
TTACAATTCC TTTGGCCCAG ACCTGTGGAG TATCTCAATA TTTACAACTT AACGATAACT 
GAGAGCATAA GCAGGGAGAC ATTTATTTAC GTGGAGACAG TGTTGAAGTC ACTGAAGATA 
GAGCATGTCA CAAACCAAGT GTTCCTCTTT GTGAAGGATG CACTATATTC TGTGTTTGCA 
GAAATGAACA TCAGGATGCT CACACTGTCA GACACGCCAT TCATCCACAT GGTGTGCCCT 
GAGTTCCCAA GCACATTTGC ATTTCTGAAC TTTACCCAGA ACGTTTTTAC TGACAGCATT 
TTTCAAGGCT GTTCCACCTT GAAGAGACTG GAGACACTTA TCTTGCAAAG GAATGGTTTA 
AAGAACCTTT TTAAGGTAGC TCTCATGACC AAGACTATGT CCTCTCTGGA AACATTGGAT 
GTCAGTTTGA ATTCTTTGAA CTCTCATGTG TATGACAGAA CATGTGCTTG GGCCGAGAGC 
ATACGGGTGT TGAATTTGTC TTCGAATGTA CTTTCGGACT CTGTCTTCAG GTGCTTACCT 
CCCAAGGTCA AGGTCCTTGA CCTTCACAAC AACAGGATAG TGAGCATCCC TAAAGATGTC 
ACCCACCTGC AAGCTTTGCA GGAACTCAAT GTCGCATCCA ATTTTTTAAC TGACCTTCCT 
GGATGTGGAG CCTTCAGTAG CCTTTCTGTG CTGGTCATCG ACCATAACTC AGTTTCCCAC 
CCCTCCTCTG ATTTCTTCCA GAGCTGTCAG AATATCAGGT CCATAACAGC GGGGAACAAC 
CCATTCCGAT GCACATGTGA GCTGAGGGAG TTTGTCAAAA ACATAGGTCA GGCATCAAGA 
GAAGTGGTGG AGGGCTGGCC TGACTCTTAC AGATGTGATT ACCCAGACAG CATTAAGGGA 
ACCCCACTGC AGGACTTCCA CATGTCTCCA CTGTCCTGCG ATACAATTCT ACTGACTGTC 
ACCATTGGGG CCACTCTGCT GCTACTGGCT GCCATTGGGG CTTCCCTCTG TCTCTACTTT 
GATCTGCCCT GGTATCTCAG GATGCTATGG CAGTGGACAC AGACCAGGCA CAGGGCCAGG 
AACATCCCCT TAGAGGAACT GCAGAGGAAC CTCCAGTTCC ATGCTTTTGT CTCATACAGT 
GAGCATGATT CTGCCTGGGT GAAGAATGAA TTACTACCAA ACCTAGAGAA AGATGACATT 
CGGGTTTGCC TCCATGAGAG AAACTTTGTC CCTGGCAAGA GCATTGTGGA GAACATCATA 
CACTTCATTG AGAAGAGTTA CAAGTCCATC TTTGTGCTGT CTCCCCACTT CATCCAGAGT 
GAGTGGTGCC ATTATGAACT CTACTTTGCC CATCACAATC TCTTCCACGA AGGGTCTGAT 
AACTTAATCC TGATCTTGCT GGAACCCATC CAACAGAACA ACATTCCCAG TAGATACCAC 
AAGCTGAGGG CTCTCATGGC ACAGCGGACT TACTTGGAAT GGCCTATTGA GAAGGGTAAA 
CGTGGGCTGT TTTGGGCCAA CCTTAGAGCT TCTTTTATTA TGAAGCTAGC CTTAGTTAAT 
GAGAATGATG TGAAAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997487.1
CDS	243..2663
Translation	MVKSLWDSLCNMSQDREPIVESFHFVCTLALIVGSMTQFSDELESVVDYSNKNLTHVPKDLSPSTKSLSLSQNSISDLQMSDISFLSELRVLRLSHNRIRRLDFGVFLLNRDLEYLDVSHNQLQNISCCPMVNLKHLDLSFNDFEVLPVYKEFGNLRKLSFLGLSAAKFRQLDLLPISHLHLSCVLLDLVNYQIKDGETESLQVPNTNVLHLVFHPNSLFSVQVNISVNALGCLQLSNIKLNDENCQSLIIFLSELTRGPTLLNLTLQHIETNWKCFVRLLQFLWPRPVEYLNIYNLTITESISRETFIYVETVLKSLKIEHVTNQVFLFVKDALYSVFAEMNIRMLTLSDTPFIHMVCPEFPSTFAFLNFTQNVFTDSIFQGCSTLKRLETLILQRNGLKNLFKVALMTKTMSSLETLDVSLNSLNSHVYDRTCAWAESIRVLNLSSNVLSDSVFRCLPPKVKVLDLHNNRIVSIPKDVTHLQALQELNVASNFLTDLPGCGAFSSLSVLVIDHNSVSHPSSDFFQSCQNIRSITAGNNPFRCTCELREFVKNIGQASREVVEGWPDSYRCDYPDSIKGTPLQDFHMSPLSCDTILLTVTIGATLLLLAAIGASLCLYFDLPWYLRMLWQWTQTRHRARNIPLEELQRNLQFHAFVSYSEHDSAWVKNELLPNLEKDDIRVCLHERNFVPGKSIVENIIHFIEKSYKSIFVLSPHFIQSEWCHYELYFAHHNLFHEGSDNLILILLEPIQQNNIPSRYHKLRALMAQRTYLEWPIEKGKRGLFWANLRASFIMKLALVNENDVKT

Target ORF information:

RefSeq Version	NM_207604.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus toll-like receptor 6 (Tlr6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207604.1

ORF Insert Sequence:

ATGGTAAAAT CCCTCTGGGA TAGCCTCTGC AACATGTCCC AAGACAGAGA ACCCATCGTG 
GAGAGTTTCC ATTTTGTTTG CACCCTGGCC TTAATAGTCG GAAGCATGAC CCAGTTCTCT 
GATGAACTTG AGTCTGTAGT AGACTATTCA AACAAGAACC TTACTCATGT CCCAAAAGAC 
CTGTCACCAA GCACAAAATC CTTGAGTCTG TCTCAAAACT CCATATCTGA TCTTCAGATG 
TCTGATATCA GCTTTCTGTC AGAGCTGAGA GTTCTGAGAC TCTCCCACAA CAGAATACGG 
AGACTTGACT TTGGTGTGTT CTTGCTCAAT CGGGACTTAG AATACCTGGA TGTCTCTCAC 
AATCAGTTAC AAAACATCTC TTGCTGTCCT ATGGTGAACT TGAAACATCT AGACCTCTCA 
TTCAATGATT TTGAAGTGCT GCCCGTGTAT AAGGAATTTG GCAACTTGAG GAAGCTGAGT 
TTCTTGGGAT TAAGTGCTGC AAAGTTCCGA CAACTGGATC TGCTCCCAAT TTCTCACTTG 
CACCTGAGCT GTGTTCTTCT GGACTTGGTG AATTATCAGA TAAAAGATGG TGAAACAGAA 
AGTCTTCAGG TTCCAAATAC CAACGTTCTC CATTTGGTCT TTCATCCGAA TAGCCTGTTC 
TCTGTGCAAG TGAACATATC TGTAAATGCT TTAGGATGCT TACAACTGAG TAATATTAAA 
TTGAATGATG AAAACTGTCA AAGCTTAATT ATATTTTTAT CAGAACTCAC CAGAGGTCCA 
ACCTTATTGA ATCTGACCCT CCAGCACATA GAAACAAACT GGAAGTGCTT TGTTAGACTT 
TTACAATTCC TTTGGCCCAG ACCTGTGGAG TATCTCAATA TTTACAACTT AACGATAACT 
GAGAGCATAA GCAGGGAGAC ATTTATTTAC GTGGAGACAG TGTTGAAGTC ACTGAAGATA 
GAGCATGTCA CAAACCAAGT GTTCCTCTTT GTGAAGGATG CACTATATTC TGTGTTTGCA 
GAAATGAACA TCAGGATGCT CACACTGTCA GACACGCCAT TCATCCACAT GGTGTGCCCT 
GAGTTCCCAA GCACATTTGC ATTTCTGAAC TTTACCCAGA ACGTTTTTAC TGACAGCATT 
TTTCAAGGCT GTTCCACCTT GAAGAGACTG GAGACACTTA TCTTGCAAAG GAATGGTTTA 
AAGAACCTTT TTAAGGTAGC TCTCATGACC AAGACTATGT CCTCTCTGGA AACATTGGAT 
GTCAGTTTGA ATTCTTTGAA CTCTCATGTG TATGACAGAA CATGTGCTTG GGCCGAGAGC 
ATACGGGTGT TGAATTTGTC TTCGAATGTA CTTTCGGACT CTGTCTTCAG GTGCTTACCT 
CCCAAGGTCA AGGTCCTTGA CCTTCACAAC AACAGGATAG TGAGCATCCC TAAAGATGTC 
ACCCACCTGC AAGCTTTGCA GGAACTCAAT GTCGCATCCA ATTTTTTAAC TGACCTTCCT 
GGATGTGGAG CCTTCAGTAG CCTTTCTGTG CTGGTCATCG ACCATAACTC AGTTTCCCAC 
CCCTCCTCTG ATTTCTTCCA GAGCTGTCAG AATATCAGGT CCATAACAGC GGGGAACAAC 
CCATTCCGAT GCACATGTGA GCTGAGGGAG TTTGTCAAAA ACATAGGTCA GGCATCAAGA 
GAAGTGGTGG AGGGCTGGCC TGACTCTTAC AGATGTGATT ACCCAGACAG CATTAAGGGA 
ACCCCACTGC AGGACTTCCA CATGTCTCCA CTGTCCTGCG ATACAATTCT ACTGACTGTC 
ACCATTGGGG CCACTCTGCT GCTACTGGCT GCCATTGGGG CTTCCCTCTG TCTCTACTTT 
GATCTGCCCT GGTATCTCAG GATGCTATGG CAGTGGACAC AGACCAGGCA CAGGGCCAGG 
AACATCCCCT TAGAGGAACT GCAGAGGAAC CTCCAGTTCC ATGCTTTTGT CTCATACAGT 
GAGCATGATT CTGCCTGGGT GAAGAATGAA TTACTACCAA ACCTAGAGAA AGATGACATT 
CGGGTTTGCC TCCATGAGAG AAACTTTGTC CCTGGCAAGA GCATTGTGGA GAACATCATA 
CACTTCATTG AGAAGAGTTA CAAGTCCATC TTTGTGCTGT CTCCCCACTT CATCCAGAGT 
GAGTGGTGCC ATTATGAACT CTACTTTGCC CATCACAATC TCTTCCACGA AGGGTCTGAT 
AACTTAATCC TGATCTTGCT GGAACCCATC CAACAGAACA ACATTCCCAG TAGATACCAC 
AAGCTGAGGG CTCTCATGGC ACAGCGGACT TACTTGGAAT GGCCTATTGA GAAGGGTAAA 
CGTGGGCTGT TTTGGGCCAA CCTTAGAGCT TCTTTTATTA TGAAGCTAGC CTTAGTTAAT 
GAGAATGATG TGAAAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tlr6 ( XM_006250960.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251022.1 Rattus norvegicus toll-like receptor 6 (Tlr6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36070
Clone ID Related Accession (Same CDS sequence)	XM_006250960.2
Accession Version	XM_006250960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	toll-like receptor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006250960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAAT CCCTCTGGGA TAGCCTCTGC AACATGTCCC AAGACAGAGA ACCCATCGTG 
GAGAGTTTCC ATTTTGTTTG CACCCTGGCC TTAATAGTCG GAAGCATGAC CCAGTTCTCT 
GATGAATTTG AGTCTGTAGT AGACTATTCA AACAAGAACC TTACTCATGT CCCAAAAGAC 
CTGTCACCAA GCACAAAATC CTTGAGTCTG TCTCAAAACT CCATATCTGA TCTTCAGATG 
TCTGATATCA GCTTTCTGTC AGAGCTGAGA GTTCTGAGAC TCTCCCACAA CAGAATACGG 
AGACTTGACT TTGGTGTGTT CTTGCTCAAT CGGGACTTAG AATACCTGGA TGTCTCTCAC 
AATCAGTTAC AAAACATCTC TTGCTGTCCT ATGGTGAACT TGAAACATCT AGACCTCTCA 
TTCAATGACT TTGAAGTGCT GCCCGTGTAT AAGGAATTTG GCAACTTGAT GAAGCTGAGT 
TTCTTGGGAT TAAGTGCTGC AAAGTTCCGA CAACTGGATC TGCTCCCAAT TTCTCACTTG 
CACCTGAGCT GTGTTCTTCT GGACTTGGTG AATTATCAGA TAAAAGATGG TGAAACAGAA 
AGTCTTCAGG TTCCAAATAC CAACGTTCTC CATTTGGTCT TTCATCCGAA TAGCCTGTTC 
TCTGTGCAAG TGAACATATC TGTAAATGCT TTAGGATGCT TACAACTGAG TAATATTAAA 
TTGAATGATA AAAACTGTCA AAGCTTAATT ATATTTTTAT CAGAACTCAC CAGAGGTCCA 
ACCTTATTGA ATCTGACCCT CCAGCACATA GAAACAAACT GGAAGTGCTT TGTTAGACTT 
TTACAATTCC TTTGGCCCAG ACCTGTGGAG TATCTCAATA TTTACAACTT AACGATAACT 
GAGAGCATAA GCAGGGAGAC ATTTATTTAC GTGGAGACGG TGTTGAAGTC ACTGAAGATA 
GAGCATGTCA CAAACCAAGT GTTCCTCTTT GTGAAGGATG CACTATATTC TGTGTTTGCA 
GAAATGAACA TCAGGATGCT CACACTGTCA GACACGCCAT TCATCCACAT GGTGTGCCCT 
GAGTTCCCAA GCACATTTGC ATTTCTGAAC TTTACCCAGA ACGTTTTTAC TGACAGCATT 
TTTCAAGGCT GTTCCACCTT GAAGAGACTG GAGACACTTA TCTTGCAAAG GAATGGTTTA 
AAGAACCTTT TTAAGGTAGC TCTCATGACC AAGACTATGT CCTCTCTGGA AACATTGGAT 
GTCAGTTTGA ATTCTTTGAA CTCTCATGTG TATGACAGAA CATGTGCTTG GGCCGAGAGC 
ATACGGGTGT TGAATTTGTC TTCGAATGTA CTTTCGGACT CTGTCTTCAG GTGCTTACCT 
CCCAAGGTCA AGGTCCTTGA CCTTCACAAC AACAGGATAG TGAGCATCCC TAAAGATGTC 
ACCCACCTGC AAGCTTTGCA GGAACTCAAT GTCGCATCCA ATTTTTTAAC TGACCTTCCT 
GGATGTGGAG CCTTCAGTAG CCTTTCTGTG CTGGTCATCG ACCATAACTC AGTTTCCCAC 
CCCTCCTCTG ATTTCTTCCA GAGCTGTCAG AATATCAGGT CCATAACAGC GGGGAACAAC 
CCATTCCGAT GCACATGTGA GCTGAGGGAG TTTGTCAAAA ACATAGGTCA GGCATCAAGA 
GAAGTGGTGG AGGGCTGGCC TGACTCTTAC AGATGTGATT ACCCAGACAG CATTAAGGGA 
ACCCCACTGC AGGACTTCCA CATGTCTCCA CTGTCCTGCG ATACAATTCT ACTGACTGTC 
ACCATTGGGG CCACTCTGCT GCTACTGGCT GCCATTGGGG CTTCCCTCTG TCTCTACTTT 
GATCTGCCCT GGTATCTCAG GATGCTATGG CAGTGGACAC AGACCAGGCA CAGGGCCAGG 
AACATCCCCT TAGAGGAACT GCAGAGGAAC CTCCAGTTCC ATGCTTTTGT CTCATACAGT 
GAGCATGATT CTGCCTGGGT GAAGAATGAA TTACTACCAA ACCTAGAGAA AGATGACATT 
CGGGTTTGCC TCCATGAGAG AAACTTTGTC CCTGGCAAGA GCATTGTGGA GAACATCATA 
CACTTCATTG AGAAGAGTTA CAAGTCCATC TTTGTGCTGT CTCCCCACTT CATCCAGAGT 
GAGTGGTGCC ATTATGAACT CTACTTTGCC CATCACAATC TCTTCCACGA AGGGTCTGAT 
AACTTAATCC TGATCTTGCT GGAACCCATC CAACAGAACA ACATTCCCAG TAGATACCAC 
AAGCTGAGGG CTCTCATGGC ACAGCGGACT TACTTGGAAT GGCCTATTGA GAAGGGTAAA 
CGTGGGCTGT TTTGGGCCAA CCTTAGAGCT TCTTTTATTA TGAAGCTAGC CTTAGTTAAT 
GAGAATGATG TGAAAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251022.1
CDS	99..2519
Translation	MVKSLWDSLCNMSQDREPIVESFHFVCTLALIVGSMTQFSDEFESVVDYSNKNLTHVPKDLSPSTKSLSLSQNSISDLQMSDISFLSELRVLRLSHNRIRRLDFGVFLLNRDLEYLDVSHNQLQNISCCPMVNLKHLDLSFNDFEVLPVYKEFGNLMKLSFLGLSAAKFRQLDLLPISHLHLSCVLLDLVNYQIKDGETESLQVPNTNVLHLVFHPNSLFSVQVNISVNALGCLQLSNIKLNDKNCQSLIIFLSELTRGPTLLNLTLQHIETNWKCFVRLLQFLWPRPVEYLNIYNLTITESISRETFIYVETVLKSLKIEHVTNQVFLFVKDALYSVFAEMNIRMLTLSDTPFIHMVCPEFPSTFAFLNFTQNVFTDSIFQGCSTLKRLETLILQRNGLKNLFKVALMTKTMSSLETLDVSLNSLNSHVYDRTCAWAESIRVLNLSSNVLSDSVFRCLPPKVKVLDLHNNRIVSIPKDVTHLQALQELNVASNFLTDLPGCGAFSSLSVLVIDHNSVSHPSSDFFQSCQNIRSITAGNNPFRCTCELREFVKNIGQASREVVEGWPDSYRCDYPDSIKGTPLQDFHMSPLSCDTILLTVTIGATLLLLAAIGASLCLYFDLPWYLRMLWQWTQTRHRARNIPLEELQRNLQFHAFVSYSEHDSAWVKNELLPNLEKDDIRVCLHERNFVPGKSIVENIIHFIEKSYKSIFVLSPHFIQSEWCHYELYFAHHNLFHEGSDNLILILLEPIQQNNIPSRYHKLRALMAQRTYLEWPIEKGKRGLFWANLRASFIMKLALVNENDVKT

Target ORF information:

RefSeq Version	XM_006250960.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus toll-like receptor 6 (Tlr6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250960.2

ORF Insert Sequence:

ATGGTAAAAT CCCTCTGGGA TAGCCTCTGC AACATGTCCC AAGACAGAGA ACCCATCGTG 
GAGAGTTTCC ATTTTGTTTG CACCCTGGCC TTAATAGTCG GAAGCATGAC CCAGTTCTCT 
GATGAATTTG AGTCTGTAGT AGACTATTCA AACAAGAACC TTACTCATGT CCCAAAAGAC 
CTGTCACCAA GCACAAAATC CTTGAGTCTG TCTCAAAACT CCATATCTGA TCTTCAGATG 
TCTGATATCA GCTTTCTGTC AGAGCTGAGA GTTCTGAGAC TCTCCCACAA CAGAATACGG 
AGACTTGACT TTGGTGTGTT CTTGCTCAAT CGGGACTTAG AATACCTGGA TGTCTCTCAC 
AATCAGTTAC AAAACATCTC TTGCTGTCCT ATGGTGAACT TGAAACATCT AGACCTCTCA 
TTCAATGACT TTGAAGTGCT GCCCGTGTAT AAGGAATTTG GCAACTTGAT GAAGCTGAGT 
TTCTTGGGAT TAAGTGCTGC AAAGTTCCGA CAACTGGATC TGCTCCCAAT TTCTCACTTG 
CACCTGAGCT GTGTTCTTCT GGACTTGGTG AATTATCAGA TAAAAGATGG TGAAACAGAA 
AGTCTTCAGG TTCCAAATAC CAACGTTCTC CATTTGGTCT TTCATCCGAA TAGCCTGTTC 
TCTGTGCAAG TGAACATATC TGTAAATGCT TTAGGATGCT TACAACTGAG TAATATTAAA 
TTGAATGATA AAAACTGTCA AAGCTTAATT ATATTTTTAT CAGAACTCAC CAGAGGTCCA 
ACCTTATTGA ATCTGACCCT CCAGCACATA GAAACAAACT GGAAGTGCTT TGTTAGACTT 
TTACAATTCC TTTGGCCCAG ACCTGTGGAG TATCTCAATA TTTACAACTT AACGATAACT 
GAGAGCATAA GCAGGGAGAC ATTTATTTAC GTGGAGACGG TGTTGAAGTC ACTGAAGATA 
GAGCATGTCA CAAACCAAGT GTTCCTCTTT GTGAAGGATG CACTATATTC TGTGTTTGCA 
GAAATGAACA TCAGGATGCT CACACTGTCA GACACGCCAT TCATCCACAT GGTGTGCCCT 
GAGTTCCCAA GCACATTTGC ATTTCTGAAC TTTACCCAGA ACGTTTTTAC TGACAGCATT 
TTTCAAGGCT GTTCCACCTT GAAGAGACTG GAGACACTTA TCTTGCAAAG GAATGGTTTA 
AAGAACCTTT TTAAGGTAGC TCTCATGACC AAGACTATGT CCTCTCTGGA AACATTGGAT 
GTCAGTTTGA ATTCTTTGAA CTCTCATGTG TATGACAGAA CATGTGCTTG GGCCGAGAGC 
ATACGGGTGT TGAATTTGTC TTCGAATGTA CTTTCGGACT CTGTCTTCAG GTGCTTACCT 
CCCAAGGTCA AGGTCCTTGA CCTTCACAAC AACAGGATAG TGAGCATCCC TAAAGATGTC 
ACCCACCTGC AAGCTTTGCA GGAACTCAAT GTCGCATCCA ATTTTTTAAC TGACCTTCCT 
GGATGTGGAG CCTTCAGTAG CCTTTCTGTG CTGGTCATCG ACCATAACTC AGTTTCCCAC 
CCCTCCTCTG ATTTCTTCCA GAGCTGTCAG AATATCAGGT CCATAACAGC GGGGAACAAC 
CCATTCCGAT GCACATGTGA GCTGAGGGAG TTTGTCAAAA ACATAGGTCA GGCATCAAGA 
GAAGTGGTGG AGGGCTGGCC TGACTCTTAC AGATGTGATT ACCCAGACAG CATTAAGGGA 
ACCCCACTGC AGGACTTCCA CATGTCTCCA CTGTCCTGCG ATACAATTCT ACTGACTGTC 
ACCATTGGGG CCACTCTGCT GCTACTGGCT GCCATTGGGG CTTCCCTCTG TCTCTACTTT 
GATCTGCCCT GGTATCTCAG GATGCTATGG CAGTGGACAC AGACCAGGCA CAGGGCCAGG 
AACATCCCCT TAGAGGAACT GCAGAGGAAC CTCCAGTTCC ATGCTTTTGT CTCATACAGT 
GAGCATGATT CTGCCTGGGT GAAGAATGAA TTACTACCAA ACCTAGAGAA AGATGACATT 
CGGGTTTGCC TCCATGAGAG AAACTTTGTC CCTGGCAAGA GCATTGTGGA GAACATCATA 
CACTTCATTG AGAAGAGTTA CAAGTCCATC TTTGTGCTGT CTCCCCACTT CATCCAGAGT 
GAGTGGTGCC ATTATGAACT CTACTTTGCC CATCACAATC TCTTCCACGA AGGGTCTGAT 
AACTTAATCC TGATCTTGCT GGAACCCATC CAACAGAACA ACATTCCCAG TAGATACCAC 
AAGCTGAGGG CTCTCATGGC ACAGCGGACT TACTTGGAAT GGCCTATTGA GAAGGGTAAA 
CGTGGGCTGT TTTGGGCCAA CCTTAGAGCT TCTTTTATTA TGAAGCTAGC CTTAGTTAAT 
GAGAATGATG TGAAAACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure of TIR domain of TLR6 reveals novel dimeric interface of TIR-TIR interaction for toll-like receptor signaling pathway.
Journal of molecular biology426(19)3305-3313(2014 Sep)
Jang TH,Park HH

Regulation of angiogenesis, mural cell recruitment and adventitial macrophage behavior by Toll-like receptors.
Angiogenesis17(1)147-61(2014 Jan)
Aplin AC,Ligresti G,Fogel E,Zorzi P,Smith K,Nicosia RF

CD36 coordinates NLRP3 inflammasome activation by facilitating intracellular nucleation of soluble ligands into particulate ligands in sterile inflammation.
Nature immunology14(8)812-20(2013 Aug)
Sheedy FJ,Grebe A,Rayner KJ,Kalantari P,Ramkhelawon B,Carpenter SB,Becker CE,Ediriweera HN,Mullick AE,Golenbock DT,Stuart LM,Latz E,Fitzgerald KA,Moore KJ

TLR2 is a primary receptor for Alzheimer's amyloid ? peptide to trigger neuroinflammatory activation.
Journal of immunology (Baltimore, Md. : 1950)188(3)1098-107(2012 Feb)
Liu S,Liu Y,Hao W,Wolf L,Kiliaan AJ,Penke B,R?be CE,Walter J,Heneka MT,Hartmann T,Menger MD,Fassbender K

Isolated Toll-like receptor transmembrane domains are capable of oligomerization.
PloS one7(11)e48875(2012)
Godfroy JI 3rd,Roostan M,Moroz YS,Korendovych IV,Yin H