Pask cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pask
Entrez Gene ID	301617
Full Name	PAS domain containing serine/threonine kinase
General protein information	Preferred Names PAS domain containing serine/threonine kinase Names PAS domain-containing serine/threonine-protein kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009362.1	NP_001009362.1	PAS domain-containing serine/threonine-protein kinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	PASK	NP_001239048.1
Canis lupus familiaris (dog)	PASK	XP_005636031.1
Mus musculus (house mouse)	Pask	NP_543126.2
Pan troglodytes (chimpanzee)	PASK	XP_003950058.1
Rattus norvegicus (Norway rat)	Pask	NP_001009362.1

Gene Ontology
Bibliography

Related articles in PubMed

Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway.
Liu YQ, Liu YF, Ma XM, Xiao YD, Wang YB, Zhang MZ, Cheng AX, Wang TT, Li JL, Zhao PX, Xie F, Zhang X
Journal of plastic, reconstructive & aesthetic surgery : JPRAS68(7)e147-56(2015 Jul)

Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.
Vorontsova I, Lam L, Delpire E, Lim J, Donaldson P
Investigative ophthalmology & visual science56(1)310-21(2014 Dec)

PAS kinase as a nutrient sensor in neuroblastoma and hypothalamic cells required for the normal expression and activity of other cellular nutrient and energy sensors.
Hurtado-Carneiro V, Roncero I, Blazquez E, Alvarez E, Sanz C
Molecular neurobiology48(3)904-20(2013 Dec)

Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3? in pancreatic ?-cells.
Semache M, Zarrouki B, Font?s G, Fogarty S, Kikani C, Chawki MB, Rutter J, Poitout V
The Journal of biological chemistry288(34)24825-33(2013 Aug)

Human mutation within Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) causes basal insulin hypersecretion.
Semplici F, Vaxillaire M, Fogarty S, Semache M, Bonnefond A, Font?s G, Philippe J, Meur G, Diraison F, Sessions RB, Rutter J, Poitout V, Froguel P, Rutter GA
The Journal of biological chemistry286(51)44005-14(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis;tissues with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion.
Title: Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway.

WNK 1, 3, 4, OSR1, and SPAK signaling system known to play a role in regulating the phosphorylation status, and hence activity of the CCCs in other tissues, is also present in the rat and human lenses.
Title: Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.

PASK is a key kinase in GLP-1 actions and exerts a coordinated response with the other metabolic sensors.
Title: PAS kinase as a nutrient sensor in neuroblastoma and hypothalamic cells required for the normal expression and activity of other cellular nutrient and energy sensors.

PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells.
Title: Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3? in pancreatic ?-cells.

Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) has a role in insulin hypersecretion
Title: Human mutation within Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) causes basal insulin hypersecretion.

The following Pask gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pask cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00793	NM_001009362.1 Latest version!	Rattus norvegicus PAS domain containing serine/threonine kinase (Pask), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pask ( NM_001009362.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009362.1 Rattus norvegicus PAS domain containing serine/threonine kinase (Pask), mRNA.

Price & Availability↑

CloneID	ORa00793
Clone ID Related Accession (Same CDS sequence)	NM_001009362.1
Accession Version	NM_001009362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	PAS domain-containing serine/threonine-protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087047.1. On Jan 7, 2005 this sequence version replaced XM_217480.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087047.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGACA GAGGTCCACC AGTTCTTGCT GAGGACTGGA AGTCTGAGAG TCTTCCTGCC 
CAAGAGGGCC CAGCTACCCA GGCTTTCGTT GAGCCCAGCA AGCCTCTCTC CATAGCCCAT 
AGACACCTGA ACAGGAAGAA TGGGCTTTCT AGACTCTGCC AGAGCCGAAT GGCACTCTCC 
GAGGATAGGT GGAGCTCTTA CTGTCTTTCA TCATTGGCTG CCCAAAACAT CTGCACAAGC 
AAACTGCACT GCGCTGCTAC ACCTGACTAT GCTGACCCTA TAGCTGGCCC ACTGGGCAGT 
ACATCGTGCT GCTCCTTGCT ACGTGGCCTA GCTTCAGGGT GCTCTGGACC TCTGCTCTCG 
ACCCCTGTGT GCAACCCTAA CAAGGCTGTC TTTACTGTGG ATGCCAAGAC CACAGAGATT 
CTGGTTGCCA ATGACAAAGC CTGCAGCCTC CTGGGGTATA GCAGTCATGA CCTGATTGGC 
CAAAAGCTTA CACAGTTCTT CCTCAAGTCT GATTCCGAGG TTGTGGAAGC CCTCAGCGAG 
GAGCATGTGG AGGCTGATGG CCACGCTGCA GTAGTGTTCG GCACGGTGGT GGACATTGTC 
AGCCGAAGTG GGGAGAAGAT TCCCGTCTCT GTGTGGATAA AGAGGTTGCA ACAAGATCGT 
GGCTTGTGCT GTGTAGTGGT TCTGGAGCCT GTGGAGAGGG TCTCAGCCTG GGTCGCCTTC 
CAGAGTGATG GAACTATCAC GTCATGCGAC AGCCTCTTTG CTCATCTTCA TGGGTTCACA 
TCTCCAAAGG ATGTGGTTGG GCAGTGTGTC ATAGACTTGA TTCCATCTAT ACAGCTCCCT 
CCTCCTGGCC AACATGTCCC AAAGAGTCTC AAGATTCAAA GGTCTGTTGG GAGAGCCAGA 
GACGGGACCA CCTTCCCTCT CAGTCTGAAG TTGAAATCTA AGACCAGTGG AAAGGCTGTA 
GCTGAAAGTG AGGTGGCCTC AGAACCAGGT TACCAGGCTT CTGTGTGGGT GTTCTGCACC 
ATCAGTGGCC TCATCACTCT CCTGCCTGAT GGCACAATTT ATGGCATCAA CCACAGCTTT 
GCACTAATGT TGTTTGGTTA TGGAAAAACA GAGCTCCTGG GCAAGAACAT CACTTGCCTG 
ATTCCTGGTT TCTACCACTG CATGGACCTT GCATATGATA GTTCTGTCCA GCTGCCAGAT 
CTGGTCAACT GCCTGGACAT TGGCAGCAAG AGTGGGCCTG GGGAGATGAG TTCTGATGCC 
AAGCAGGACT GGGAGCTGGC CAGTGGGGCC CAAGATCCAA GAATTGATGT GTTGACACGG 
GGCCACATGC CATCACAAGA TGAGACCCTG AAGCTGCGGG GAGGACAAGT CTCGCCTAGG 
ACTCAGACTC AGCTGGAAAC TGGACGCCAC ATACTTCCTT CTTGTGCATC TCAGCCCTCC 
CTGGGGGTAG ACAGCAGCCC AGTGGACGGT GAGCGGTCAT TACCCACAGA CCAGCGGAAC 
GTTCCAAAGA GAAACCTGCT AGCCCATGCT GAACAGTTAC TATCTCAGAC CCAACCAGAC 
ACCCTGGAAA TATCCCCACC AGTCCTTAAA GAACTGTCTG AAATTCAGAA AAATATCTCA 
GAAGAAAGCC CACTAACCCA CAGGAAATGG TTATTGTCCA AGGCCCAGAA AGACACCACA 
AAAGGAAGCC TGCCAATCCA TGATGAGAAG TTACTGCTTG CAAGCCAGCA TATCCATGTC 
TTGGGAAAGG AAAATCCTTC AGCAGCAGAG AGCTACAGGG AGGGTCTTTT AGCAGAAAGC 
AGGTCCAAAC CAGTGGATGC AAAACCGTTT GCTTCATGTG AAGATTCTGA GCTTCTAGTC 
CCAGCTAAGG ACAGAAGCAA CAGAGCGGAC ATGAGTAGTC TGCAACAGGA AGCTCAATTG 
GAGCTGATGG GTATCAGTAG TCCCGGTAGT CCTAACCCCT GGGCTGATGC CACCATGCCT 
GAGCCCCACA CCACAGGGCA GATACCAGGA GGGAGCCTGA CATACTGCCC ACAGCATAGA 
AGCGAGTGGA TCTTACAACA GCGAGGCCAG GACTCGGCTC CCAGCCCCTC TGCAATGGGG 
TGTGTCTTTG TTGGGACACC CACTCTGGAT GAACCATGGC CAGGAGTGAG AAATGATCGA 
GAAGAGCTGC AGACTTGCTT GATTAAGGAA CAGTTGTCCA AGTCAAGCTT TGGGGGAGCT 
CTAGGAATCT CCTGTGTGGA ACTTGTCCCA GCAGAACATC CTCCTTTCAC TGCCCCCGTG 
TCCTTCTGTG ATTGGGGAGG CCGAGACCTA CATGCTAGCC GCTCAGGCAG CTCCTCAGCC 
TGCTATGCTT TGGCCACTGA CCTCCCTGGT GTCTTGGAGG CAGTGGAAGC CCAGGAGGCT 
GATGTGAGTT CTTATTCCTG GAACCTCAGG GAGCTCTTTC TCAATGACCG GACAGACAGA 
ACTCCATCAC ATTGTTCCTG TACCACTTCT GAACTTAGCG AGACACCATC TCTTTCAGGG 
GTGGGGTCAG ATTTAGATGT GGGTATTCTG CACAGGCAGA CTTCAGATAT TTTAGCTCAC 
CGAGAGATGT TGCTTCTTAG TGGGACCTAT TTTGACCTTA GGGAAGGCCA TCGGTTCCAG 
GAGATGGGAG CAGGACATGA TGGAGCAGAA CTGTCTAACA TTTCTTTGGT ATCCTCTGAA 
CACTATGAAA CTAGTGACAT AGAAAGCCTA GGTTGTGACC CTCCCATACC AGATCCTGGT 
CCTAACAATA TGTGCCTATC AGCAGAGAAG CCTAGACTGA GTGCCCAGAT CACTTCCACT 
CCTGTGGCAC CTGGGCCCAC CAGCCTGCAG CAGGAGATCC AGGAGGGCAT CTTCTCTGGA 
AGCTGCTACC ACCGAGATGG CTTACGACTG AGTGTACAGT TTGAAGTAAA GCGTGTGCAG 
CTCCAGGGCT CTGCAACCCT GTTCTGCTGC TGGCTGGTGA AGGACTTCTT ACACAGTCAC 
CGGGACTCAG CCACCCGAAC CCGCCTGTTC CTTGCCAGCC TGCCAAGCTC TACCCACTCC 
ATGCCTGACC TCTCTGGATC CAGCTTTGGA GAGATGCTCA GAGCCAAGCC TTGGTTTGAG 
GAGTCCCCAA CACCCGCAGA GCTGGAGGGG CTGGCAGCCT GTGAGGGCGA GTACTCCCAT 
AAGTACAACA CCCTGCGCCC ACTCGGCAGT GGCGCCTTTG GCTTTGTGTG GACTGCAGTG 
GAGAAGGAAG GCAACAAAGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTTTTAGAG 
GATTGTTGGG TTGAAGATCC CAAACTTGGA AGAGTTCCTT TAGAGATTTC AATTCTGTCC 
AAGGTGGAAC ATGCCAACAT AATCAAGATT GTGGATGTAT TCGAAAACCA AGGGTTCTTT 
CAGCTAGTGA TGGAAAAGCA TGGCTCTGGC TTGGACCTCT TTGCTTTCAT TGACCGTCAT 
CCCTGCTTGG ATGAGCCCCT GGCGAGTTAC ATCTTCCGAC AACTGGTGTC TGCTGTAAGA 
TACTTGCGTT CCCAAGGCAT CATCCATAGA GACATCAAGG ATGAGAACAT TGTGATTGCT 
GAGGACTTCA CAATTAAGCT GATAGATTTT GGCTCAGCTG CCTACTTAGA GAAGGGCAAA 
CTATTTTATA CCTTTTGTGG AACAATTGAA TACTGTGCAC CTGAAGTTCT CATTGGAAAT 
CCCTACAGAG GGCCAGAGCT GGAGATGTGG TCTCTCGGGG TCACGCTGTA CACGCTCATC 
TTCGAGGAGA ATCCCTTCTG TGAGGTGGAG GAGACCATGG AGGCAGTTAT TCATCCCCCC 
TTCCTGGTTT CCCAAGAACT TATGAGTCTT CTGTCTGGGC TGCTGCAGCC TTGCCCTGAG 
CAGCGGACCA CTTTGGAGAA GCTGATTGTG GACCCCTGGG TGACACAGCC TGTGAACCTT 
GATAACTACA CCTGGGAAGA GGTGTGTCGG ACCAGCCAGC CAGAAAGTGG CCTGATGTCA 
GCTGCAAGTC TGGAGCTTGC GAGTAGGAGT CCAAGTGAAG TGGCTCAGAC AGAGGGTCTT 
TGTAGACCTC CTGCTCCCAA GGAGGCTCCT GGTAACCACT GCTTGCATCT TGAGGATCCC 
TCGTCACCAG TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009362.1
CDS	119..4276
Translation	MEDRGPPVLAEDWKSESLPAQEGPATQAFVEPSKPLSIAHRHLNRKNGLSRLCQSRMALSEDRWSSYCLSSLAAQNICTSKLHCAATPDYADPIAGPLGSTSCCSLLRGLASGCSGPLLSTPVCNPNKAVFTVDAKTTEILVANDKACSLLGYSSHDLIGQKLTQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRSGEKIPVSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSIQLPPPGQHVPKSLKIQRSVGRARDGTTFPLSLKLKSKTSGKAVAESEVASEPGYQASVWVFCTISGLITLLPDGTIYGINHSFALMLFGYGKTELLGKNITCLIPGFYHCMDLAYDSSVQLPDLVNCLDIGSKSGPGEMSSDAKQDWELASGAQDPRIDVLTRGHMPSQDETLKLRGGQVSPRTQTQLETGRHILPSCASQPSLGVDSSPVDGERSLPTDQRNVPKRNLLAHAEQLLSQTQPDTLEISPPVLKELSEIQKNISEESPLTHRKWLLSKAQKDTTKGSLPIHDEKLLLASQHIHVLGKENPSAAESYREGLLAESRSKPVDAKPFASCEDSELLVPAKDRSNRADMSSLQQEAQLELMGISSPGSPNPWADATMPEPHTTGQIPGGSLTYCPQHRSEWILQQRGQDSAPSPSAMGCVFVGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSFGGALGISCVELVPAEHPPFTAPVSFCDWGGRDLHASRSGSSSACYALATDLPGVLEAVEAQEADVSSYSWNLRELFLNDRTDRTPSHCSCTTSELSETPSLSGVGSDLDVGILHRQTSDILAHREMLLLSGTYFDLREGHRFQEMGAGHDGAELSNISLVSSEHYETSDIESLGCDPPIPDPGPNNMCLSAEKPRLSAQITSTPVAPGPTSLQQEIQEGIFSGSCYHRDGLRLSVQFEVKRVQLQGSATLFCCWLVKDFLHSHRDSATRTRLFLASLPSSTHSMPDLSGSSFGEMLRAKPWFEESPTPAELEGLAACEGEYSHKYNTLRPLGSGAFGFVWTAVEKEGNKEVVVKFIKKEKVLEDCWVEDPKLGRVPLEISILSKVEHANIIKIVDVFENQGFFQLVMEKHGSGLDLFAFIDRHPCLDEPLASYIFRQLVSAVRYLRSQGIIHRDIKDENIVIAEDFTIKLIDFGSAAYLEKGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIVDPWVTQPVNLDNYTWEEVCRTSQPESGLMSAASLELASRSPSEVAQTEGLCRPPAPKEAPGNHCLHLEDPSSPVS

Target ORF information:

RefSeq Version	NM_001009362.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PAS domain containing serine/threonine kinase (Pask), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009362.1

ORF Insert Sequence:

ATGGAGGACA GAGGTCCACC AGTTCTTGCT GAGGACTGGA AGTCTGAGAG TCTTCCTGCC 
CAAGAGGGCC CAGCTACCCA GGCTTTCGTT GAGCCCAGCA AGCCTCTCTC CATAGCCCAT 
AGACACCTGA ACAGGAAGAA TGGGCTTTCT AGACTCTGCC AGAGCCGAAT GGCACTCTCC 
GAGGATAGGT GGAGCTCTTA CTGTCTTTCA TCATTGGCTG CCCAAAACAT CTGCACAAGC 
AAACTGCACT GCGCTGCTAC ACCTGACTAT GCTGACCCTA TAGCTGGCCC ACTGGGCAGT 
ACATCGTGCT GCTCCTTGCT ACGTGGCCTA GCTTCAGGGT GCTCTGGACC TCTGCTCTCG 
ACCCCTGTGT GCAACCCTAA CAAGGCTGTC TTTACTGTGG ATGCCAAGAC CACAGAGATT 
CTGGTTGCCA ATGACAAAGC CTGCAGCCTC CTGGGGTATA GCAGTCATGA CCTGATTGGC 
CAAAAGCTTA CACAGTTCTT CCTCAAGTCT GATTCCGAGG TTGTGGAAGC CCTCAGCGAG 
GAGCATGTGG AGGCTGATGG CCACGCTGCA GTAGTGTTCG GCACGGTGGT GGACATTGTC 
AGCCGAAGTG GGGAGAAGAT TCCCGTCTCT GTGTGGATAA AGAGGTTGCA ACAAGATCGT 
GGCTTGTGCT GTGTAGTGGT TCTGGAGCCT GTGGAGAGGG TCTCAGCCTG GGTCGCCTTC 
CAGAGTGATG GAACTATCAC GTCATGCGAC AGCCTCTTTG CTCATCTTCA TGGGTTCACA 
TCTCCAAAGG ATGTGGTTGG GCAGTGTGTC ATAGACTTGA TTCCATCTAT ACAGCTCCCT 
CCTCCTGGCC AACATGTCCC AAAGAGTCTC AAGATTCAAA GGTCTGTTGG GAGAGCCAGA 
GACGGGACCA CCTTCCCTCT CAGTCTGAAG TTGAAATCTA AGACCAGTGG AAAGGCTGTA 
GCTGAAAGTG AGGTGGCCTC AGAACCAGGT TACCAGGCTT CTGTGTGGGT GTTCTGCACC 
ATCAGTGGCC TCATCACTCT CCTGCCTGAT GGCACAATTT ATGGCATCAA CCACAGCTTT 
GCACTAATGT TGTTTGGTTA TGGAAAAACA GAGCTCCTGG GCAAGAACAT CACTTGCCTG 
ATTCCTGGTT TCTACCACTG CATGGACCTT GCATATGATA GTTCTGTCCA GCTGCCAGAT 
CTGGTCAACT GCCTGGACAT TGGCAGCAAG AGTGGGCCTG GGGAGATGAG TTCTGATGCC 
AAGCAGGACT GGGAGCTGGC CAGTGGGGCC CAAGATCCAA GAATTGATGT GTTGACACGG 
GGCCACATGC CATCACAAGA TGAGACCCTG AAGCTGCGGG GAGGACAAGT CTCGCCTAGG 
ACTCAGACTC AGCTGGAAAC TGGACGCCAC ATACTTCCTT CTTGTGCATC TCAGCCCTCC 
CTGGGGGTAG ACAGCAGCCC AGTGGACGGT GAGCGGTCAT TACCCACAGA CCAGCGGAAC 
GTTCCAAAGA GAAACCTGCT AGCCCATGCT GAACAGTTAC TATCTCAGAC CCAACCAGAC 
ACCCTGGAAA TATCCCCACC AGTCCTTAAA GAACTGTCTG AAATTCAGAA AAATATCTCA 
GAAGAAAGCC CACTAACCCA CAGGAAATGG TTATTGTCCA AGGCCCAGAA AGACACCACA 
AAAGGAAGCC TGCCAATCCA TGATGAGAAG TTACTGCTTG CAAGCCAGCA TATCCATGTC 
TTGGGAAAGG AAAATCCTTC AGCAGCAGAG AGCTACAGGG AGGGTCTTTT AGCAGAAAGC 
AGGTCCAAAC CAGTGGATGC AAAACCGTTT GCTTCATGTG AAGATTCTGA GCTTCTAGTC 
CCAGCTAAGG ACAGAAGCAA CAGAGCGGAC ATGAGTAGTC TGCAACAGGA AGCTCAATTG 
GAGCTGATGG GTATCAGTAG TCCCGGTAGT CCTAACCCCT GGGCTGATGC CACCATGCCT 
GAGCCCCACA CCACAGGGCA GATACCAGGA GGGAGCCTGA CATACTGCCC ACAGCATAGA 
AGCGAGTGGA TCTTACAACA GCGAGGCCAG GACTCGGCTC CCAGCCCCTC TGCAATGGGG 
TGTGTCTTTG TTGGGACACC CACTCTGGAT GAACCATGGC CAGGAGTGAG AAATGATCGA 
GAAGAGCTGC AGACTTGCTT GATTAAGGAA CAGTTGTCCA AGTCAAGCTT TGGGGGAGCT 
CTAGGAATCT CCTGTGTGGA ACTTGTCCCA GCAGAACATC CTCCTTTCAC TGCCCCCGTG 
TCCTTCTGTG ATTGGGGAGG CCGAGACCTA CATGCTAGCC GCTCAGGCAG CTCCTCAGCC 
TGCTATGCTT TGGCCACTGA CCTCCCTGGT GTCTTGGAGG CAGTGGAAGC CCAGGAGGCT 
GATGTGAGTT CTTATTCCTG GAACCTCAGG GAGCTCTTTC TCAATGACCG GACAGACAGA 
ACTCCATCAC ATTGTTCCTG TACCACTTCT GAACTTAGCG AGACACCATC TCTTTCAGGG 
GTGGGGTCAG ATTTAGATGT GGGTATTCTG CACAGGCAGA CTTCAGATAT TTTAGCTCAC 
CGAGAGATGT TGCTTCTTAG TGGGACCTAT TTTGACCTTA GGGAAGGCCA TCGGTTCCAG 
GAGATGGGAG CAGGACATGA TGGAGCAGAA CTGTCTAACA TTTCTTTGGT ATCCTCTGAA 
CACTATGAAA CTAGTGACAT AGAAAGCCTA GGTTGTGACC CTCCCATACC AGATCCTGGT 
CCTAACAATA TGTGCCTATC AGCAGAGAAG CCTAGACTGA GTGCCCAGAT CACTTCCACT 
CCTGTGGCAC CTGGGCCCAC CAGCCTGCAG CAGGAGATCC AGGAGGGCAT CTTCTCTGGA 
AGCTGCTACC ACCGAGATGG CTTACGACTG AGTGTACAGT TTGAAGTAAA GCGTGTGCAG 
CTCCAGGGCT CTGCAACCCT GTTCTGCTGC TGGCTGGTGA AGGACTTCTT ACACAGTCAC 
CGGGACTCAG CCACCCGAAC CCGCCTGTTC CTTGCCAGCC TGCCAAGCTC TACCCACTCC 
ATGCCTGACC TCTCTGGATC CAGCTTTGGA GAGATGCTCA GAGCCAAGCC TTGGTTTGAG 
GAGTCCCCAA CACCCGCAGA GCTGGAGGGG CTGGCAGCCT GTGAGGGCGA GTACTCCCAT 
AAGTACAACA CCCTGCGCCC ACTCGGCAGT GGCGCCTTTG GCTTTGTGTG GACTGCAGTG 
GAGAAGGAAG GCAACAAAGA GGTGGTGGTG AAGTTTATTA AGAAGGAGAA GGTTTTAGAG 
GATTGTTGGG TTGAAGATCC CAAACTTGGA AGAGTTCCTT TAGAGATTTC AATTCTGTCC 
AAGGTGGAAC ATGCCAACAT AATCAAGATT GTGGATGTAT TCGAAAACCA AGGGTTCTTT 
CAGCTAGTGA TGGAAAAGCA TGGCTCTGGC TTGGACCTCT TTGCTTTCAT TGACCGTCAT 
CCCTGCTTGG ATGAGCCCCT GGCGAGTTAC ATCTTCCGAC AACTGGTGTC TGCTGTAAGA 
TACTTGCGTT CCCAAGGCAT CATCCATAGA GACATCAAGG ATGAGAACAT TGTGATTGCT 
GAGGACTTCA CAATTAAGCT GATAGATTTT GGCTCAGCTG CCTACTTAGA GAAGGGCAAA 
CTATTTTATA CCTTTTGTGG AACAATTGAA TACTGTGCAC CTGAAGTTCT CATTGGAAAT 
CCCTACAGAG GGCCAGAGCT GGAGATGTGG TCTCTCGGGG TCACGCTGTA CACGCTCATC 
TTCGAGGAGA ATCCCTTCTG TGAGGTGGAG GAGACCATGG AGGCAGTTAT TCATCCCCCC 
TTCCTGGTTT CCCAAGAACT TATGAGTCTT CTGTCTGGGC TGCTGCAGCC TTGCCCTGAG 
CAGCGGACCA CTTTGGAGAA GCTGATTGTG GACCCCTGGG TGACACAGCC TGTGAACCTT 
GATAACTACA CCTGGGAAGA GGTGTGTCGG ACCAGCCAGC CAGAAAGTGG CCTGATGTCA 
GCTGCAAGTC TGGAGCTTGC GAGTAGGAGT CCAAGTGAAG TGGCTCAGAC AGAGGGTCTT 
TGTAGACCTC CTGCTCCCAA GGAGGCTCCT GGTAACCACT GCTTGCATCT TGAGGATCCC 
TCGTCACCAG TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway.
Journal of plastic, reconstructive & aesthetic surgery : JPRAS68(7)e147-56(2015 Jul)
Liu YQ,Liu YF,Ma XM,Xiao YD,Wang YB,Zhang MZ,Cheng AX,Wang TT,Li JL,Zhao PX,Xie F,Zhang X

Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.
Investigative ophthalmology & visual science56(1)310-21(2014 Dec)
Vorontsova I,Lam L,Delpire E,Lim J,Donaldson P

PAS kinase as a nutrient sensor in neuroblastoma and hypothalamic cells required for the normal expression and activity of other cellular nutrient and energy sensors.
Molecular neurobiology48(3)904-20(2013 Dec)
Hurtado-Carneiro V,Roncero I,Blazquez E,Alvarez E,Sanz C

Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3? in pancreatic ?-cells.
The Journal of biological chemistry288(34)24825-33(2013 Aug)
Semache M,Zarrouki B,Font?s G,Fogarty S,Kikani C,Chawki MB,Rutter J,Poitout V

Human mutation within Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) causes basal insulin hypersecretion.
The Journal of biological chemistry286(51)44005-14(2011 Dec)
Semplici F,Vaxillaire M,Fogarty S,Semache M,Bonnefond A,Font?s G,Philippe J,Meur G,Diraison F,Sessions RB,Rutter J,Poitout V,Froguel P,Rutter GA

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Pask cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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