Cisd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cisd1
Entrez Gene ID	294362
Full Name	CDGSH iron sulfur domain 1
Synonyms	MitoNEET,RGD1309529
General protein information	Preferred Names CDGSH iron sulfur domain 1 Names CDGSH iron-sulfur domain-containing protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601618.1	XP_017457107.1	CDGSH iron-sulfur domain-containing protein 1 isoform X1
NM_001106385.2	NP_001099855.1	CDGSH iron-sulfur domain-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CISD1	NP_001238954.1
Rattus norvegicus (Norway rat)	Cisd1	NP_001099855.1
Gallus gallus (chicken)	CISD1	NP_001186458.1
Danio rerio (zebrafish)	cisd1	NP_956899.1
	CISD1	XP_001097858.1
Canis lupus familiaris (dog)	LOC102154780	XP_005618779.1
Homo sapiens (human)	CISD1	NP_060934.1
Mus musculus (house mouse)	Cisd1	NP_598768.1
Bos taurus (cattle)	CISD1	NP_001029229.1
Caenorhabditis elegans (roundworm)	W02B12.15	NP_001122648.1
Xenopus tropicalis (tropical clawed frog)	cisd1	NP_001004811.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection.
He QQ, Xiong LL, Liu F, He X, Feng GY, Shang FF, Xia QJ, Wang YC, Qiu DL, Luo CZ, Liu J, Wang TH
Scientific reports635205(2016 Oct)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Chapter 13 Localization and function of the 2Fe-2S outer mitochondrial membrane protein mitoNEET.
Wiley SE, Rardin MJ, Dixon JE
Methods in enzymology456233-46(2009)

MitoNEET is a uniquely folded 2Fe 2S outer mitochondrial membrane protein stabilized by pioglitazone.
Paddock ML, Wiley SE, Axelrod HL, Cohen AE, Roy M, Abresch EC, Capraro D, Murphy AN, Nechushtai R, Dixon JE, Jennings PA
Proceedings of the National Academy of Sciences of the United States of America104(36)14342-7(2007 Sep)

Crystal structure of human mitoNEET reveals distinct groups of iron sulfur proteins.
Lin J, Zhou T, Ye K, Wang J
Proceedings of the National Academy of Sciences of the United States of America104(37)14640-5(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

describes methods for isolating mitochondrial membrane fractions that are enriched in mitoNEET, generating constructs for the expression of recombinant mitoNEET protein and analyzing the 2Fe-2S cluster of mitoNEET in vitro
Title: Chapter 13 Localization and function of the 2Fe-2S outer mitochondrial membrane protein mitoNEET.

mito- NEET is an important iron-containing protein involved in the control of maximal mitochondrial respiratory rates [mitoNEET]
Title: MitoNEET is an iron-containing outer mitochondrial membrane protein that regulates oxidative capacity.

mitoNEET is located in mitochondrial fraction of rat brain, liver and skeletal muscle.
Title: Identification of a novel mitochondrial protein ("mitoNEET") cross-linked specifically by a thiazolidinedione photoprobe.

The following Cisd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cisd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10938	NM_001106385.2 Latest version!	Rattus norvegicus CDGSH iron sulfur domain 1 (Cisd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa60086	XM_017601618.1 Latest version!	Rattus norvegicus CDGSH iron sulfur domain 1 (Cisd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cisd1 ( NM_001106385.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099855.1 Rattus norvegicus CDGSH iron sulfur domain 1 (Cisd1), mRNA.

Price & Availability↑

CloneID	ORa10938
Clone ID Related Accession (Same CDS sequence)	NM_001106385.2
Accession Version	NM_001106385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	CDGSH iron-sulfur domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159419.1. On Feb 17, 2008 this sequence version replaced NM_001106385.1. ##Evidence-Data-START## Transcript exon combination :: BC159419.1, DY319784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCTCA GCTCCGACTC CCCTGTGCGA GTTGAGTGGA TCGCCGCCGT GACCTTTGCT 
GCTGGCACAG CCGCTCTCGG TTACCTGGCT TACAAGAAGT TCTACGCTAA AGAGAGTCGC 
ACCAAAGCTA TGGTGAACCT TCAGATCCAG AAAGACAACC CGAAGGTTGT GCACGCCTTC 
GACATGGAGG ATCTGGGGGA TAAGGCCGTG TATTGCCGGT GCTGGAGGTC TAAAAAGTTC 
CCGTTCTGCG ATGGGGCTCA CATAAAGCAC AATGAGGAGA CTGGAGACAA CGTGGGACCT 
CTGATCATCA AGAAAAAAGA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099855.1
CDS	87..413
Translation	MGLSSDSPVRVEWIAAVTFAAGTAALGYLAYKKFYAKESRTKAMVNLQIQKDNPKVVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHIKHNEETGDNVGPLIIKKKET

Target ORF information:

RefSeq Version	NM_001106385.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDGSH iron sulfur domain 1 (Cisd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106385.2

ORF Insert Sequence:

ATGGGCCTCA GCTCCGACTC CCCTGTGCGA GTTGAGTGGA TCGCCGCCGT GACCTTTGCT 
GCTGGCACAG CCGCTCTCGG TTACCTGGCT TACAAGAAGT TCTACGCTAA AGAGAGTCGC 
ACCAAAGCTA TGGTGAACCT TCAGATCCAG AAAGACAACC CGAAGGTTGT GCACGCCTTC 
GACATGGAGG ATCTGGGGGA TAAGGCCGTG TATTGCCGGT GCTGGAGGTC TAAAAAGTTC 
CCGTTCTGCG ATGGGGCTCA CATAAAGCAC AATGAGGAGA CTGGAGACAA CGTGGGACCT 
CTGATCATCA AGAAAAAAGA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cisd1 ( XM_017601618.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457107.1 Rattus norvegicus CDGSH iron sulfur domain 1 (Cisd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60086
Clone ID Related Accession (Same CDS sequence)	XM_017601618.1
Accession Version	XM_017601618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	CDGSH iron-sulfur domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGGT CCCGCCAAGA AACTGTTGAG TGGATCGCCG CCGTGACCTT TGCTGCTGGC 
ACAGCCGCTC TCGGTTACCT GGCTTACAAG AAGTTCTACG CTAAAGAGAG TCGCACCAAA 
GCTATGGTGA ACCTTCAGAT CCAGAAAGAC AACCCGAAGG TTGTGCACGC CTTCGACATG 
GAGGATCTGG GGGATAAGGC CGTGTATTGC CGGTGCTGGA GGTCTAAAAA GTTCCCGTTC 
TGCGATGGGG CTCACATAAA GCACAATGAG GAGACTGGAG ACAACGTGGG ACCTCTGATC 
ATCAAGAAAA AAGAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457107.1
CDS	246..566
Translation	MYRSRQETVEWIAAVTFAAGTAALGYLAYKKFYAKESRTKAMVNLQIQKDNPKVVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHIKHNEETGDNVGPLIIKKKET

Target ORF information:

RefSeq Version	XM_017601618.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CDGSH iron sulfur domain 1 (Cisd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601618.1

ORF Insert Sequence:

ATGTACAGGT CCCGCCAAGA AACTGTTGAG TGGATCGCCG CCGTGACCTT TGCTGCTGGC 
ACAGCCGCTC TCGGTTACCT GGCTTACAAG AAGTTCTACG CTAAAGAGAG TCGCACCAAA 
GCTATGGTGA ACCTTCAGAT CCAGAAAGAC AACCCGAAGG TTGTGCACGC CTTCGACATG 
GAGGATCTGG GGGATAAGGC CGTGTATTGC CGGTGCTGGA GGTCTAAAAA GTTCCCGTTC 
TGCGATGGGG CTCACATAAA GCACAATGAG GAGACTGGAG ACAACGTGGG ACCTCTGATC 
ATCAAGAAAA AAGAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-127 targeting of mitoNEET inhibits neurite outgrowth, induces cell apoptosis and contributes to physiological dysfunction after spinal cord transection.
Scientific reports635205(2016 Oct)
He QQ,Xiong LL,Liu F,He X,Feng GY,Shang FF,Xia QJ,Wang YC,Qiu DL,Luo CZ,Liu J,Wang TH

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

Chapter 13 Localization and function of the 2Fe-2S outer mitochondrial membrane protein mitoNEET.
Methods in enzymology456233-46(2009)
Wiley SE,Rardin MJ,Dixon JE

MitoNEET is a uniquely folded 2Fe 2S outer mitochondrial membrane protein stabilized by pioglitazone.
Proceedings of the National Academy of Sciences of the United States of America104(36)14342-7(2007 Sep)
Paddock ML,Wiley SE,Axelrod HL,Cohen AE,Roy M,Abresch EC,Capraro D,Murphy AN,Nechushtai R,Dixon JE,Jennings PA

Crystal structure of human mitoNEET reveals distinct groups of iron sulfur proteins.
Proceedings of the National Academy of Sciences of the United States of America104(37)14640-5(2007 Sep)
Lin J,Zhou T,Ye K,Wang J

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Cisd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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