CISD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CISD1
Entrez Gene ID	55847
Full Name	CDGSH iron sulfur domain 1
Synonyms	C10orf70,MDS029,ZCD1,mitoNEET
General protein information	Preferred Names CDGSH iron sulfur domain 1 Names CDGSH iron-sulfur domain-containing protein 1 zinc finger CDGSH-type domain 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q21.1
Summary	This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012].
Disorder MIM:	611932

mRNA and Protein(s)

mRNA	Protein	Name
NM_018464.5	NP_060934.1	CDGSH iron-sulfur domain-containing protein 1
XM_017016409.1	XP_016871898.1	CDGSH iron-sulfur domain-containing protein 1 isoform X1
NM_018464.4	NP_060934.1	CDGSH iron-sulfur domain-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CISD1	NP_001238954.1
Rattus norvegicus (Norway rat)	Cisd1	NP_001099855.1
Gallus gallus (chicken)	CISD1	NP_001186458.1
Danio rerio (zebrafish)	cisd1	NP_956899.1
	CISD1	XP_001097858.1
Canis lupus familiaris (dog)	LOC102154780	XP_005618779.1
Homo sapiens (human)	CISD1	NP_060934.1
Mus musculus (house mouse)	Cisd1	NP_598768.1
Bos taurus (cattle)	CISD1	NP_001029229.1
Caenorhabditis elegans (roundworm)	W02B12.15	NP_001122648.1
Xenopus tropicalis (tropical clawed frog)	cisd1	NP_001004811.1

Gene Ontology
Bibliography

Related articles in PubMed

Anamorsin/Ndor1 Complex Reduces [2Fe-2S]-MitoNEET via a Transient Protein-Protein Interaction.
Camponeschi F, Ciofi-Baffoni S, Banci L
Journal of the American Chemical Society139(28)9479-9482(2017 Jul)

The mitochondrial outer membrane protein mitoNEET is a redox enzyme catalyzing electron transfer from FMNH
Wang Y, Landry AP, Ding H
The Journal of biological chemistry292(24)10061-10067(2017 06)

Flavin nucleotides act as electron shuttles mediating reduction of the [2Fe-2S] clusters in mitochondrial outer membrane protein mitoNEET.
Landry AP, Wang Y, Cheng Z, Crochet RB, Lee YH, Ding H
Free radical biology & medicine102240-247(2017 Jan)

CISD1 inhibits ferroptosis by protection against mitochondrial lipid peroxidation.
Yuan H, Li X, Zhang X, Kang R, Tang D
Biochemical and biophysical research communications478(2)838-44(2016 09)

Redox Control of the Human Iron-Sulfur Repair Protein MitoNEET Activity via Its Iron-Sulfur Cluster.
Golinelli-Cohen MP, Lescop E, Mons C, Gon?alves S, Cl?mancey M, Santolini J, Guittet E, Blondin G, Latour JM, Bouton C
The Journal of biological chemistry291(14)7583-93(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that, compared with oxygen, ubiquinone-2 is more efficient in oxidizing mitoNEET [2Fe-2S] clusters, suggesting that ubiquinone could be an intrinsic electron acceptor of reduced mitoNEET [2Fe-2S] clusters in mitochondrial outer membrane.
Title: The mitochondrial outer membrane protein mitoNEET is a redox enzyme catalyzing electron transfer from FMNH

The results suggest that flavin nucleotides may act as electron shuttles to reduce the mitoNEET [2Fe-2S] clusters and regulate mitochondrial functions in human cells.
Title: Flavin nucleotides act as electron shuttles mediating reduction of the [2Fe-2S] clusters in mitochondrial outer membrane protein mitoNEET.

CISD1 inhibits ferroptosis by protecting the cells against mitochondrial lipid peroxidation.
Title: CISD1 inhibits ferroptosis by protection against mitochondrial lipid peroxidation.

the redox-sensing function of mNT is a key component of the cellular adaptive response to help stress-sensitive Fe-S proteins recover from oxidative injury.
Title: Redox Control of the Human Iron-Sulfur Repair Protein MitoNEET Activity via Its Iron-Sulfur Cluster.

Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process.
Title: His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase.

The following CISD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CISD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21485	NM_018464.5 Latest version!	Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu91516	XM_017016409.1 Latest version!	Homo sapiens CDGSH iron sulfur domain 1 (CISD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu21485	NM_018464.4	Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CISD1 ( NM_018464.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060934.1 Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.

Price & Availability↑

CloneID	OHu21485
Clone ID Related Accession (Same CDS sequence)	NM_018464.5 , NM_018464.4
Accession Version	NM_018464.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	CDGSH iron-sulfur domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA417986.1, AY960578.1, AC016396.6 and AI025360.1. On Nov 22, 2018 this sequence version replaced NM_018464.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG614903.1, SRR1660803.175955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333926.6/ ENSP00000363041.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTCTGA CTTCCAGTTC CAGCGTACGA GTTGAATGGA TCGCAGCAGT TACCATTGCT 
GCTGGGACAG CTGCAATTGG TTATCTAGCT TACAAAAGAT TTTATGTTAA AGATCATCGA 
AATAAAGCTA TGATAAACCT TCACATCCAG AAAGACAACC CCAAGATAGT ACATGCTTTT 
GACATGGAGG ATTTGGGAGA TAAAGCTGTG TACTGCCGTT GTTGGAGGTC CAAAAAGTTC 
CCATTCTGTG ATGGGGCTCA CACAAAACAT AACGAAGAGA CTGGAGACAA TGTGGGCCCT 
CTGATCATCA AGAAAAAAGA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060934.1
CDS	113..439
Translation	MSLTSSSSVRVEWIAAVTIAAGTAAIGYLAYKRFYVKDHRNKAMINLHIQKDNPKIVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHTKHNEETGDNVGPLIIKKKET

Target ORF information:

RefSeq Version	NM_018464.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018464.5

ORF Insert Sequence:

ATGAGTCTGA CTTCCAGTTC CAGCGTACGA GTTGAATGGA TCGCAGCAGT TACCATTGCT 
GCTGGGACAG CTGCAATTGG TTATCTAGCT TACAAAAGAT TTTATGTTAA AGATCATCGA 
AATAAAGCTA TGATAAACCT TCACATCCAG AAAGACAACC CCAAGATAGT ACATGCTTTT 
GACATGGAGG ATTTGGGAGA TAAAGCTGTG TACTGCCGTT GTTGGAGGTC CAAAAAGTTC 
CCATTCTGTG ATGGGGCTCA CACAAAACAT AACGAAGAGA CTGGAGACAA TGTGGGCCCT 
CTGATCATCA AGAAAAAAGA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CISD1 ( NM_018464.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060934.1 Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.

Price & Availability↑

CloneID	OHu21485
Clone ID Related Accession (Same CDS sequence)	NM_018464.5 , NM_018464.4
Accession Version	NM_018464.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	CDGSH iron-sulfur domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA417986.1, AC016396.6, AY960578.1 and AI025360.1. On Feb 4, 2012 this sequence version replaced NM_018464.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.25246.1, SRR1660803.175955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGA CTTCCAGTTC CAGCGTACGA GTTGAATGGA TCGCAGCAGT TACCATTGCT 
GCTGGGACAG CTGCAATTGG TTATCTAGCT TACAAAAGAT TTTATGTTAA AGATCATCGA 
AATAAAGCTA TGATAAACCT TCACATCCAG AAAGACAACC CCAAGATAGT ACATGCTTTT 
GACATGGAGG ATTTGGGAGA TAAAGCTGTG TACTGCCGTT GTTGGAGGTC CAAAAAGTTC 
CCATTCTGTG ATGGGGCTCA CACAAAACAT AACGAAGAGA CTGGAGACAA TGTGGGCCCT 
CTGATCATCA AGAAAAAAGA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060934.1
CDS	173..499
Translation	MSLTSSSSVRVEWIAAVTIAAGTAAIGYLAYKRFYVKDHRNKAMINLHIQKDNPKIVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHTKHNEETGDNVGPLIIKKKET

Target ORF information:

RefSeq Version	NM_018464.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDGSH iron sulfur domain 1 (CISD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018464.4

ORF Insert Sequence:

ATGAGTCTGA CTTCCAGTTC CAGCGTACGA GTTGAATGGA TCGCAGCAGT TACCATTGCT 
GCTGGGACAG CTGCAATTGG TTATCTAGCT TACAAAAGAT TTTATGTTAA AGATCATCGA 
AATAAAGCTA TGATAAACCT TCACATCCAG AAAGACAACC CCAAGATAGT ACATGCTTTT 
GACATGGAGG ATTTGGGAGA TAAAGCTGTG TACTGCCGTT GTTGGAGGTC CAAAAAGTTC 
CCATTCTGTG ATGGGGCTCA CACAAAACAT AACGAAGAGA CTGGAGACAA TGTGGGCCCT 
CTGATCATCA AGAAAAAAGA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CISD1 ( XM_017016409.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871898.1 Homo sapiens CDGSH iron sulfur domain 1 (CISD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu91516
Clone ID Related Accession (Same CDS sequence)	XM_017016409.1
Accession Version	XM_017016409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	CDGSH iron-sulfur domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTAT GGCAGTTGAT GGTGGGCTGG AGAGGGCAGT TTAGAGCAGA AAAGGGGCTA 
AGCCCACTCC ATGCTGGGAA TAGACAACTC TCCTCTGAGG CTGAGGCTAA CCAGATTATG 
CAGAACTTTC TTCTCTCCAA CATTGCCTGG AGCTGGGTTG AATGGATCGC AGCAGTTACC 
ATTGCTGCTG GGACAGCTGC AATTGGTTAT CTAGCTTACA AAAGATTTTA TGTTAAAGAT 
CATCGAAATA AAGCTATGAT AAACCTTCAC ATCCAGAAAG ACAACCCCAA GATAGTACAT 
GCTTTTGACA TGGAGGATTT GGGAGATAAA GCTGTGTACT GCCGTTGTTG GAGGTCCAAA 
AAGTTCCCAT TCTGTGATGG GGCTCACACA AAACATAACG AAGAGACTGG AGACAATGTG 
GGCCCTCTGA TCATCAAGAA AAAAGAAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871898.1
CDS	288..740
Translation	MILWQLMVGWRGQFRAEKGLSPLHAGNRQLSSEAEANQIMQNFLLSNIAWSWVEWIAAVTIAAGTAAIGYLAYKRFYVKDHRNKAMINLHIQKDNPKIVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHTKHNEETGDNVGPLIIKKKET

Target ORF information:

RefSeq Version	XM_017016409.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CDGSH iron sulfur domain 1 (CISD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016409.1

ORF Insert Sequence:

ATGATTCTAT GGCAGTTGAT GGTGGGCTGG AGAGGGCAGT TTAGAGCAGA AAAGGGGCTA 
AGCCCACTCC ATGCTGGGAA TAGACAACTC TCCTCTGAGG CTGAGGCTAA CCAGATTATG 
CAGAACTTTC TTCTCTCCAA CATTGCCTGG AGCTGGGTTG AATGGATCGC AGCAGTTACC 
ATTGCTGCTG GGACAGCTGC AATTGGTTAT CTAGCTTACA AAAGATTTTA TGTTAAAGAT 
CATCGAAATA AAGCTATGAT AAACCTTCAC ATCCAGAAAG ACAACCCCAA GATAGTACAT 
GCTTTTGACA TGGAGGATTT GGGAGATAAA GCTGTGTACT GCCGTTGTTG GAGGTCCAAA 
AAGTTCCCAT TCTGTGATGG GGCTCACACA AAACATAACG AAGAGACTGG AGACAATGTG 
GGCCCTCTGA TCATCAAGAA AAAAGAAACT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Anamorsin/Ndor1 Complex Reduces [2Fe-2S]-MitoNEET via a Transient Protein-Protein Interaction.
Journal of the American Chemical Society139(28)9479-9482(2017 Jul)
Camponeschi F,Ciofi-Baffoni S,Banci L

The mitochondrial outer membrane protein mitoNEET is a redox enzyme catalyzing electron transfer from FMNH
The Journal of biological chemistry292(24)10061-10067(2017 06)
Wang Y,Landry AP,Ding H

Flavin nucleotides act as electron shuttles mediating reduction of the [2Fe-2S] clusters in mitochondrial outer membrane protein mitoNEET.
Free radical biology & medicine102240-247(2017 Jan)
Landry AP,Wang Y,Cheng Z,Crochet RB,Lee YH,Ding H

CISD1 inhibits ferroptosis by protection against mitochondrial lipid peroxidation.
Biochemical and biophysical research communications478(2)838-44(2016 09)
Yuan H,Li X,Zhang X,Kang R,Tang D

Redox Control of the Human Iron-Sulfur Repair Protein MitoNEET Activity via Its Iron-Sulfur Cluster.
The Journal of biological chemistry291(14)7583-93(2016 Apr)
Golinelli-Cohen MP,Lescop E,Mons C,Gon?alves S,Cl?mancey M,Santolini J,Guittet E,Blondin G,Latour JM,Bouton C

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CISD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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