Ddah2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddah2
Entrez Gene ID	294239
Full Name	dimethylarginine dimethylaminohydrolase 2
General protein information	Preferred Names dimethylarginine dimethylaminohydrolase 2 Names N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 DDAH-2 DDAHII dimethylargininase-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	human and mouse homologs regulate the concentration of methylarginines [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772715.2	XP_008770937.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 isoform X1
NM_212532.2	NP_997697.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
NM_001165936.1	NP_001159408.1	N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDAH2	XP_001159196.1
Canis lupus familiaris (dog)	DDAH2	XP_005627218.1
	DDAH2	XP_001113141.1
Homo sapiens (human)	DDAH2	NP_039268.1
Danio rerio (zebrafish)	ddah2	NP_001093535.1
Xenopus tropicalis (tropical clawed frog)	ddah2	NP_001004924.1
Bos taurus (cattle)	DDAH2	NP_001029876.1
Mus musculus (house mouse)	Ddah2	NP_001177378.1
Rattus norvegicus (Norway rat)	Ddah2	NP_001159408.1

Gene Ontology
Bibliography

Related articles in PubMed

Elevated Levels of ADMA Are Associated with Lower DDAH2 and Higher PRMT1 in LPS-Induced Endometritis Rats.
Xiao HB, Sui GG, Lu XY, Sun ZL
Inflammation41(1)299-306(2018 Feb)

Protective effects of low-dose rosuvastatin on isoproterenol-induced chronic heart failure in rats by regulation of DDAH-ADMA-NO pathway.
Zhou R, Ma P, Xiong A, Xu Y, Wang Y, Xu Q
Cardiovascular therapeutics35(2)(2017 Apr)

Phosphorylation of Nonmuscle Myosin Light Chain Promotes Endothelial Injury in Hyperlipidemic Rats Through a Mechanism Involving Downregulation of Dimethylarginine Dimethylaminohydrolase 2.
Wu Y, Zhang JJ, Li TB, Liu WQ, Li LS, Luo XJ, Jiang JL, Ma QL, Yang ZC, Peng J
Journal of cardiovascular pharmacology and therapeutics21(6)536-548(2016 Nov)

The association of adipose-derived dimethylarginine dimethylaminohydrolase-2 with insulin sensitivity in experimental type 2 diabetes mellitus.
Zheng J, Wang K, Jin P, Dong C, Yuan Q, Li Y, Yang Z
Acta biochimica et biophysica Sinica45(8)641-8(2013 Aug)

Involvement of DDAH/ADMA pathway in the pathogenesis of rheumatoid arthritis in rats.
Chen XM, Xia J, Zhou T, Yuan Q, Zhang WF, Hu CP, Li YJ, Jiang JL
International immunopharmacology16(2)322-31(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low-dose rosuvastatin exerted cardioprotective effects on isoproterenol-induced heart failure in rats by modulating DDAH-ADMA-NO pathway.
Title: Protective effects of low-dose rosuvastatin on isoproterenol-induced chronic heart failure in rats by regulation of DDAH-ADMA-NO pathway.

Suppression of DDAH2 expression might account for, at least partially, the vascular endothelial dysfunction in hyperlipidemia, and nmMLC20 plays a role in suppression of DDAH2 expression in a phosphorylation-dependent manner.
Title: Phosphorylation of Nonmuscle Myosin Light Chain Promotes Endothelial Injury in Hyperlipidemic Rats Through a Mechanism Involving Downregulation of Dimethylarginine Dimethylaminohydrolase 2.

findings suggested that in the development of type 2 diabetes mellitus, local DDAH-2 in adipocytes might play an important role in regulating insulin sensitivity
Title: The association of adipose-derived dimethylarginine dimethylaminohydrolase-2 with insulin sensitivity in experimental type 2 diabetes mellitus.

The DDAH/asymmetric dimethylarginine pathway participates in the pathogenesis of rheumatoid arthritis and may be mediated by cortistatin.
Title: Involvement of DDAH/ADMA pathway in the pathogenesis of rheumatoid arthritis in rats.

downregulation of DDAH2 and eNOS induced endothelial dysfunction in sinoaortic-denervated
Title: Down-regulation of DDAH2 and eNOS induces endothelial dysfunction in sinoaortic-denervated rats.

The following Ddah2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddah2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12163	NM_001165936.1 Latest version!	Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa12163	NM_212532.2 Latest version!	Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa45193	XM_008772715.2 Latest version!	Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddah2 ( NM_212532.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997697.1 Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12163
Clone ID Related Accession (Same CDS sequence)	NM_212532.2 , NM_001165936.1
Accession Version	NM_212532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Rattus norvegicus(Norway rat)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CO385374.1 and BC097930.1. On Sep 30, 2009 this sequence version replaced NM_212532.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC097930.1, FQ213713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGACGC CGGGGGAGGG GCTGGGTCGC TGTTCCCATG CCCTGATCCG GGGTGTCCCC 
GAGAGCTTGG CATCCGGGGA AGGTGCTGGC GCTGGTCTTC CGGCTCTGGA TCTGGCTAAA 
GCTCAAAGGG AGCATGGAGT ACTAGGAGGT AAACTGAGGC AACGACTAGG TCTGCAGCTT 
CTTGAACTGC CTCCTGAGGA ATCACTGCCG CTGGGACCAC TGCTTGGTGA CACGGCTGTG 
ATCCAAGGAG ACACGGCTCT AATCACAAGG CCCTGGAGCC CAGCGCGTAG GCCTGAGGTT 
GATGGAGTCC GCAAAGCTCT CCAGGACTTG GGGCTCAGAA TTGTGGAGAT GGGGGATGAG 
AACGCTACGC TGGACGGCAC CGACGTCCTC TTCACCGGCC GGGAGTTTTT CGTAGGCCTC 
TCCAAGTGGA CCAATCATCG AGGAGCTGAG ATCGTGGCAG ACACGTTCCG GGACTTCGCT 
GTCTCTACGG TACCGGTCTC TGGCGCCTCG CATCTGCGCG GCCTCTGTGG CATGGGAGGA 
CCTCGCACGG TGGTGGCTGG AAGCAGTGAG GCTGCCCAAA AAGCCGTCAG GGCAATGGCA 
GCACTGACTG ATCACCCCTA CGCCTCTCTG ACCCTCCCAG ATGACGCAGC GAGTGACTGT 
CTCTTTCTGC GTCCTGGGTT GCCTGGTACC ACACCTTTCC TTCTGCACCG CGGAGGTGGG 
GACCTGCCCA ACAGCCAGGA GGCTCTGCAA AAGCTCTCTG ACGTCACCCT GGTACCTGTG 
TCCTGCTCGG AACTGGAGAA GGTTGGAGCT GGCCTCAGCT CCCTCTGCCT GGTGCTCAGC 
ACACGCCCCC ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997697.1
CDS	248..1105
Translation	MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEMGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGASHLRGLCGMGGPRTVVAGSSEAAQKAVRAMAALTDHPYASLTLPDDAASDCLFLRPGLPGTTPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKVGAGLSSLCLVLSTRPHC

Target ORF information:

RefSeq Version	NM_212532.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212532.2

ORF Insert Sequence:

ATGGGGACGC CGGGGGAGGG GCTGGGTCGC TGTTCCCATG CCCTGATCCG GGGTGTCCCC 
GAGAGCTTGG CATCCGGGGA AGGTGCTGGC GCTGGTCTTC CGGCTCTGGA TCTGGCTAAA 
GCTCAAAGGG AGCATGGAGT ACTAGGAGGT AAACTGAGGC AACGACTAGG TCTGCAGCTT 
CTTGAACTGC CTCCTGAGGA ATCACTGCCG CTGGGACCAC TGCTTGGTGA CACGGCTGTG 
ATCCAAGGAG ACACGGCTCT AATCACAAGG CCCTGGAGCC CAGCGCGTAG GCCTGAGGTT 
GATGGAGTCC GCAAAGCTCT CCAGGACTTG GGGCTCAGAA TTGTGGAGAT GGGGGATGAG 
AACGCTACGC TGGACGGCAC CGACGTCCTC TTCACCGGCC GGGAGTTTTT CGTAGGCCTC 
TCCAAGTGGA CCAATCATCG AGGAGCTGAG ATCGTGGCAG ACACGTTCCG GGACTTCGCT 
GTCTCTACGG TACCGGTCTC TGGCGCCTCG CATCTGCGCG GCCTCTGTGG CATGGGAGGA 
CCTCGCACGG TGGTGGCTGG AAGCAGTGAG GCTGCCCAAA AAGCCGTCAG GGCAATGGCA 
GCACTGACTG ATCACCCCTA CGCCTCTCTG ACCCTCCCAG ATGACGCAGC GAGTGACTGT 
CTCTTTCTGC GTCCTGGGTT GCCTGGTACC ACACCTTTCC TTCTGCACCG CGGAGGTGGG 
GACCTGCCCA ACAGCCAGGA GGCTCTGCAA AAGCTCTCTG ACGTCACCCT GGTACCTGTG 
TCCTGCTCGG AACTGGAGAA GGTTGGAGCT GGCCTCAGCT CCCTCTGCCT GGTGCTCAGC 
ACACGCCCCC ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah2 ( NM_001165936.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001159408.1 Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12163
Clone ID Related Accession (Same CDS sequence)	NM_212532.2 , NM_001165936.1
Accession Version	NM_001165936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Rattus norvegicus(Norway rat)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EV770973.1 and BC097930.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGACGC CGGGGGAGGG GCTGGGTCGC TGTTCCCATG CCCTGATCCG GGGTGTCCCC 
GAGAGCTTGG CATCCGGGGA AGGTGCTGGC GCTGGTCTTC CGGCTCTGGA TCTGGCTAAA 
GCTCAAAGGG AGCATGGAGT ACTAGGAGGT AAACTGAGGC AACGACTAGG TCTGCAGCTT 
CTTGAACTGC CTCCTGAGGA ATCACTGCCG CTGGGACCAC TGCTTGGTGA CACGGCTGTG 
ATCCAAGGAG ACACGGCTCT AATCACAAGG CCCTGGAGCC CAGCGCGTAG GCCTGAGGTT 
GATGGAGTCC GCAAAGCTCT CCAGGACTTG GGGCTCAGAA TTGTGGAGAT GGGGGATGAG 
AACGCTACGC TGGACGGCAC CGACGTCCTC TTCACCGGCC GGGAGTTTTT CGTAGGCCTC 
TCCAAGTGGA CCAATCATCG AGGAGCTGAG ATCGTGGCAG ACACGTTCCG GGACTTCGCT 
GTCTCTACGG TACCGGTCTC TGGCGCCTCG CATCTGCGCG GCCTCTGTGG CATGGGAGGA 
CCTCGCACGG TGGTGGCTGG AAGCAGTGAG GCTGCCCAAA AAGCCGTCAG GGCAATGGCA 
GCACTGACTG ATCACCCCTA CGCCTCTCTG ACCCTCCCAG ATGACGCAGC GAGTGACTGT 
CTCTTTCTGC GTCCTGGGTT GCCTGGTACC ACACCTTTCC TTCTGCACCG CGGAGGTGGG 
GACCTGCCCA ACAGCCAGGA GGCTCTGCAA AAGCTCTCTG ACGTCACCCT GGTACCTGTG 
TCCTGCTCGG AACTGGAGAA GGTTGGAGCT GGCCTCAGCT CCCTCTGCCT GGTGCTCAGC 
ACACGCCCCC ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159408.1
CDS	201..1058
Translation	MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEMGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGASHLRGLCGMGGPRTVVAGSSEAAQKAVRAMAALTDHPYASLTLPDDAASDCLFLRPGLPGTTPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKVGAGLSSLCLVLSTRPHC

Target ORF information:

RefSeq Version	NM_001165936.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165936.1

ORF Insert Sequence:

ATGGGGACGC CGGGGGAGGG GCTGGGTCGC TGTTCCCATG CCCTGATCCG GGGTGTCCCC 
GAGAGCTTGG CATCCGGGGA AGGTGCTGGC GCTGGTCTTC CGGCTCTGGA TCTGGCTAAA 
GCTCAAAGGG AGCATGGAGT ACTAGGAGGT AAACTGAGGC AACGACTAGG TCTGCAGCTT 
CTTGAACTGC CTCCTGAGGA ATCACTGCCG CTGGGACCAC TGCTTGGTGA CACGGCTGTG 
ATCCAAGGAG ACACGGCTCT AATCACAAGG CCCTGGAGCC CAGCGCGTAG GCCTGAGGTT 
GATGGAGTCC GCAAAGCTCT CCAGGACTTG GGGCTCAGAA TTGTGGAGAT GGGGGATGAG 
AACGCTACGC TGGACGGCAC CGACGTCCTC TTCACCGGCC GGGAGTTTTT CGTAGGCCTC 
TCCAAGTGGA CCAATCATCG AGGAGCTGAG ATCGTGGCAG ACACGTTCCG GGACTTCGCT 
GTCTCTACGG TACCGGTCTC TGGCGCCTCG CATCTGCGCG GCCTCTGTGG CATGGGAGGA 
CCTCGCACGG TGGTGGCTGG AAGCAGTGAG GCTGCCCAAA AAGCCGTCAG GGCAATGGCA 
GCACTGACTG ATCACCCCTA CGCCTCTCTG ACCCTCCCAG ATGACGCAGC GAGTGACTGT 
CTCTTTCTGC GTCCTGGGTT GCCTGGTACC ACACCTTTCC TTCTGCACCG CGGAGGTGGG 
GACCTGCCCA ACAGCCAGGA GGCTCTGCAA AAGCTCTCTG ACGTCACCCT GGTACCTGTG 
TCCTGCTCGG AACTGGAGAA GGTTGGAGCT GGCCTCAGCT CCCTCTGCCT GGTGCTCAGC 
ACACGCCCCC ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddah2 ( XM_008772715.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770937.1 Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45193
Clone ID Related Accession (Same CDS sequence)	XM_008772715.2
Accession Version	XM_008772715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACGC CGGGGGAGGG GCTGGGTCGC TGTTCCCATG CCCTGATCCG GGGTGTCCCC 
GAGAGCTTGG CATCCGGGGA AGGTGCTGGC GCTGGTCTTC CGGCTCTGGA TCTGGCTAAA 
GCTCAAAGGG AGCATGGAGT ACTAGGAGGT AAACTGAGGC AACGACTAGG TCTGCAGCTT 
CTTGAACTGC CTCCTGAGGA ATCACTGCCG CTGGGACCAC TGCTTGGTGA CACGGCTGTG 
ATCCAAGGAG ACACGGCTCT AATCACAAGG CCCTGGAGCC CAGCGCGTAG GCCTGAGGTT 
GATGGAGTCC GCAAAGCTCT CCAGGACTTG GGGCTCAGAA TTGTGGAGAT GGGGGATGAG 
AACGCTACGC TGGACGGCAC CGACGTCCTC TTCACCGGCC GGGAGTTTTT CGTAGGCCTC 
TCCAAGTGGA CCAATCATCG AGGAGCTGAG ATCGTGGCAG ACACGTTCCG GGACTTCGCT 
GTCTCTACGG TACCGGTCTC TGGCGCCTCG CATCTGCGCG GCCTCTGTGG CATGGGAGGA 
CCTCGCACGG TGGTGGCTGG AAGCAGTGAG GCTGCCCAAA AAGCCGTCAG GATGACGCAG 
CGAGTGACTG TCTCTTTCTG CGTCCTGGGT TGCCTGGTAC CACACCTTTC CTTCTGCACC 
GCGGAGGTGG GGACCTGCCC AACAGCCAGG AGGCTCTGCA AAAGCTCTCT GACGTCACCC 
TGGTACCTGT GTCCTGCTCG GAACTGGAGA AGGTTGGAGC TGGCCTCAGC TCCCTCTGCC 
TGGTGCTCAG CACACGCCCC CACTGCTGAG GGCTGGGTTT GGGGCTCCAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770937.1
CDS	515..1348
Translation	MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEMGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGASHLRGLCGMGGPRTVVAGSSEAAQKAVRMTQRVTVSFCVLGCLVPHLSFCTAEVGTCPTARRLCKSSLTSPWYLCPARNWRRLELASAPSAWCSAHAPTAEGWVWGSN

Target ORF information:

RefSeq Version	XM_008772715.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dimethylarginine dimethylaminohydrolase 2 (Ddah2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772715.2

ORF Insert Sequence:

ATGGGGACGC CGGGGGAGGG GCTGGGTCGC TGTTCCCATG CCCTGATCCG GGGTGTCCCC 
GAGAGCTTGG CATCCGGGGA AGGTGCTGGC GCTGGTCTTC CGGCTCTGGA TCTGGCTAAA 
GCTCAAAGGG AGCATGGAGT ACTAGGAGGT AAACTGAGGC AACGACTAGG TCTGCAGCTT 
CTTGAACTGC CTCCTGAGGA ATCACTGCCG CTGGGACCAC TGCTTGGTGA CACGGCTGTG 
ATCCAAGGAG ACACGGCTCT AATCACAAGG CCCTGGAGCC CAGCGCGTAG GCCTGAGGTT 
GATGGAGTCC GCAAAGCTCT CCAGGACTTG GGGCTCAGAA TTGTGGAGAT GGGGGATGAG 
AACGCTACGC TGGACGGCAC CGACGTCCTC TTCACCGGCC GGGAGTTTTT CGTAGGCCTC 
TCCAAGTGGA CCAATCATCG AGGAGCTGAG ATCGTGGCAG ACACGTTCCG GGACTTCGCT 
GTCTCTACGG TACCGGTCTC TGGCGCCTCG CATCTGCGCG GCCTCTGTGG CATGGGAGGA 
CCTCGCACGG TGGTGGCTGG AAGCAGTGAG GCTGCCCAAA AAGCCGTCAG GATGACGCAG 
CGAGTGACTG TCTCTTTCTG CGTCCTGGGT TGCCTGGTAC CACACCTTTC CTTCTGCACC 
GCGGAGGTGG GGACCTGCCC AACAGCCAGG AGGCTCTGCA AAAGCTCTCT GACGTCACCC 
TGGTACCTGT GTCCTGCTCG GAACTGGAGA AGGTTGGAGC TGGCCTCAGC TCCCTCTGCC 
TGGTGCTCAG CACACGCCCC CACTGCTGAG GGCTGGGTTT GGGGCTCCAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elevated Levels of ADMA Are Associated with Lower DDAH2 and Higher PRMT1 in LPS-Induced Endometritis Rats.
Inflammation41(1)299-306(2018 Feb)
Xiao HB,Sui GG,Lu XY,Sun ZL

Protective effects of low-dose rosuvastatin on isoproterenol-induced chronic heart failure in rats by regulation of DDAH-ADMA-NO pathway.
Cardiovascular therapeutics35(2)(2017 Apr)
Zhou R,Ma P,Xiong A,Xu Y,Wang Y,Xu Q

Phosphorylation of Nonmuscle Myosin Light Chain Promotes Endothelial Injury in Hyperlipidemic Rats Through a Mechanism Involving Downregulation of Dimethylarginine Dimethylaminohydrolase 2.
Journal of cardiovascular pharmacology and therapeutics21(6)536-548(2016 Nov)
Wu Y,Zhang JJ,Li TB,Liu WQ,Li LS,Luo XJ,Jiang JL,Ma QL,Yang ZC,Peng J

The association of adipose-derived dimethylarginine dimethylaminohydrolase-2 with insulin sensitivity in experimental type 2 diabetes mellitus.
Acta biochimica et biophysica Sinica45(8)641-8(2013 Aug)
Zheng J,Wang K,Jin P,Dong C,Yuan Q,Li Y,Yang Z

Involvement of DDAH/ADMA pathway in the pathogenesis of rheumatoid arthritis in rats.
International immunopharmacology16(2)322-31(2013 Jun)
Chen XM,Xia J,Zhou T,Yuan Q,Zhang WF,Hu CP,Li YJ,Jiang JL