Numb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Numb
Entrez Gene ID	29419
Full Name	NUMB, endocytic adaptor protein
General protein information	Preferred Names NUMB, endocytic adaptor protein Names protein numb homolog numb gene homolog numb homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	putative inhibitor of Notch signaling that is involved in neural differentiation and maturation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133287.1	NP_579821.1	protein numb homolog

Pathways from BioSystems

Homologies

	NUMB	XP_001090008.1
Canis lupus familiaris (dog)	NUMB	XP_867854.1
Mus musculus (house mouse)	Numb	NP_001129547.1
Rattus norvegicus (Norway rat)	Numb	NP_579821.1
Gallus gallus (chicken)	NUMB	NP_990166.1
Homo sapiens (human)	NUMB	NP_001005743.1
Pan troglodytes (chimpanzee)	NUMB	XP_001151152.2
Danio rerio (zebrafish)	numb	NP_001035496.1
Bos taurus (cattle)	NUMB	NP_001095421.1
Xenopus tropicalis (tropical clawed frog)	numb	NP_001090828.1

Gene Ontology
Bibliography

Related articles in PubMed

Pattern of Notch2, Numb, and Cas8 Gene Expression in Relevant Structures of the Rat Brain during Formation of Spatial Memory.
Gruden' MA, Storozheva ZI, Ratmirov AM, Sherstnev VV
Bulletin of experimental biology and medicine163(6)785-788(2017 Oct)

Numb directs the subcellular localization of EAAT3 through binding the YxNxxF motif.
Su JF, Wei J, Li PS, Miao HH, Ma YC, Qu YX, Xu J, Qin J, Li BL, Song BL, Xu ZP, Luo J
Journal of cell science129(16)3104-14(2016 08)

Numb deficiency in cerebellar Purkinje cells impairs synaptic expression of metabotropic glutamate receptor and motor coordination.
Zhou L, Yang D, Wang DJ, Xie YJ, Zhou JH, Zhou L, Huang H, Han S, Shao CY, Li HS, Zhu JJ, Qiu MS, De Zeeuw CI, Shen Y
Proceedings of the National Academy of Sciences of the United States of America112(50)15474-9(2015 Dec)

Numb induces e-cadherin adhesion dissolution, cytoskeleton reorganization, and migration in tubular epithelial cells contributing to renal fibrosis.
Ding X, Ma M, Teng J, Shao F, Teng RK, Zhou S, Zhang Y, Wu E, Wang X
Current molecular medicine15(4)368-79(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif.
Title: Numb directs the subcellular localization of EAAT3 through binding the YxNxxF motif.

This work identifies Numb as an important player in renal fibrosis, by regulating epithelial-to-mesenchymal transition (EMT) process including E-cadherin adhesion dissolution, actin reorganization, and migration enhancement in NRK52E cells.
Title: Numb induces e-cadherin adhesion dissolution, cytoskeleton reorganization, and migration in tubular epithelial cells contributing to renal fibrosis.

Numb expression is upregulated during cerebral ischemia and promotes apoptosis.
Title: Numb/Notch signaling plays an important role in cerebral ischemia-induced apoptosis.

Numb functions as a protective molecule on PA-induced apoptosis through antagonizing Notch signaling activity.
Title: Numb protects renal proximal tubular cells from puromycin aminonucleoside-induced apoptosis through inhibiting Notch signaling pathway.

four isoforms of the Numb have dynamic expression patterns during cochlear development
Title: Expression of Numb and Numb-like in the development of mammalian auditory sensory epithelium.

The following Numb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Numb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00543	NM_133287.1 Latest version!	Rattus norvegicus NUMB, endocytic adaptor protein (Numb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Numb ( NM_133287.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_579821.1 Rattus norvegicus NUMB, endocytic adaptor protein (Numb), mRNA.

Price & Availability↑

CloneID	ORa00543
Clone ID Related Accession (Same CDS sequence)	NM_133287.1
Accession Version	NM_133287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	protein numb homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB210108.1. On Nov 23, 2005 this sequence version replaced XM_234394.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB210108.1, DQ336703.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAACAAAC TACGGCAGAG TTTCAGGAGA AAGAAAGATG TTTACGTCCC AGAGGCCAGC 
CGTCCACATC AGTGGCAGAC AGATGAAGAG GGAGTCCGCA CTGGAAAGTG CAGCTTCCCA 
GTTAAGTACC TTGGCCACGT AGAGGTTGAT GAGTCAAGAG GAATGCACAT CTGTGAAGAT 
GCCGTCAAAA GATTGAAAGC TACGGGAAAG AAAGCAGTGA AGGCCGTTCT GTGGGTATCA 
GCAGATGGAC TCAGAGTTGT GGATGAAAAA ACTAAGGACC TCATAGTTGA CCAGACAATA 
GAAAAAGTTT CTTTCTGCGC CCCCGATAGG AACTTTGACA GAGCCTTTTC TTACATATGT 
CGAGATGGCA CCACTCGGCG ATGGATCTGT CATTGCTTCA TGGCTGTCAA AGACACGGGG 
GAAAGACTGA GCCATGCCGT GGGCTGTGCT TTTGCAGCCT GTTTAGAGCG TAAACAGAAG 
CGGGAAAAGG AGTGTGGAGT CACTGCTACT TTCGATGCCA GTAGAACCAC TTTTACAAGA 
GAAGGATCAT TCCGTGTCAC AACTGCCACA GAACAAGCTG AAAGAGAGGA GATCATGAAA 
CAGTTGCAAG ATGCCAAGAA AGCTGAGACA GATAAGACCG TTGGTCCATC AGTGGCTCCT 
GGCAACAGTG CTCCATCGCC GTCCTCTCCC ACCTCCCCAA CTCTGGATCC CACTGCTTCT 
TTAGAGATGA ACAATCCTCA TGCTATCCCA CGCCGGCATG CACCAATTGA ACAGCTTGCT 
CGCCAAGGCT CTTTCCGGGG ATTTCCTGCT CTTAGCCAGA AGATGTCACC CTTTAAACGC 
CAGCTGTCCC TACGCATCAA TGAGCTGCCT TCCACTATGC AGAGGAAGAC TGATTTCCCA 
ATAAAAAACA CAGTGCCTGA GGTGGAAGGA GAGGCAGAAA GCATCAGCTC CCTGTGCTCC 
CAGATCACCA GTGCCTTCAG CACACCCTGT GAGGACCCCT TCTCCTCTGC CCCAATGACC 
AAACCAGTGA CATTAGTGGC ACCACAGTCT CCTGTGTTAC AAGGGACTGA GTGGGGTCAG 
TCTTCTGGTG CTGCCTCTCC AGGTCTCTTC CAGGCTGGTC ACAGACGCAC TCCCTCTGAG 
GCTGACCGTT GGTTAGAAGA AGTATCAAAA AGTGTGCGGG CCCAGCAGCC GCAGGCCTCA 
GCCGCCCCTC TGCAGCCAGT TCTGCAGCCT CCTCCGCCCG CTGCCATTGC CCCTCCAGCA 
CCTCCTTTCC AAGGACATGC ATTCCTCACT TCTCAGCCTG TGCCAGTGGG TGTGGTCCCA 
CCCCTACAAC CAGCCTTTGT CTCTACCCAG TCCTACCCTG TGGCCAATGG GATGCCCTAT 
CCAGCCTCTA ATGTGCCTGT AGTGGGCATC ACCCCATCCC AGATGGTAGC CAATGTGTTT 
GGCACTGCAG GCCATCCTCA GGCCACTCAT CCACATCAGT CCCCAAGCCT GGCCAAGCAG 
CAGACATTCC CTCAATATGA GACAAGTAGT GCTACCACCA GTCCCTTCTT TAAGCCTTCT 
GCTCAGCACC TCAATGGTTC TGCAGCTTTC AATGGTGTAG ACAATAGCGG GCTAGTCTCA 
GGAAACAGAC CTGCACAAGT CCCTCCAGGC ACCTGCCCAG TGGATCCTTT TGAGGCCCAG 
TGGGCTGCAC TAGAAAGCAA GCCCAAGCAG CGCACCAACC CCTCTCCTAC CAACCCTTTC 
TCCAGTGATG CACAGAAGGC ATTTGAAATA GAGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579821.1
CDS	1..1779
Translation	MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKATGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQLQDAKKAETDKTVGPSVAPGNSAPSPSSPTSPTLDPTASLEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPCEDPFSSAPMTKPVTLVAPQSPVLQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQASAAPLQPVLQPPPPAAIAPPAPPFQGHAFLTSQPVPVGVVPPLQPAFVSTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQATHPHQSPSLAKQQTFPQYETSSATTSPFFKPSAQHLNGSAAFNGVDNSGLVSGNRPAQVPPGTCPVDPFEAQWAALESKPKQRTNPSPTNPFSSDAQKAFEIEL

Target ORF information:

RefSeq Version	NM_133287.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NUMB, endocytic adaptor protein (Numb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133287.1

ORF Insert Sequence:

ATGAACAAAC TACGGCAGAG TTTCAGGAGA AAGAAAGATG TTTACGTCCC AGAGGCCAGC 
CGTCCACATC AGTGGCAGAC AGATGAAGAG GGAGTCCGCA CTGGAAAGTG CAGCTTCCCA 
GTTAAGTACC TTGGCCACGT AGAGGTTGAT GAGTCAAGAG GAATGCACAT CTGTGAAGAT 
GCCGTCAAAA GATTGAAAGC TACGGGAAAG AAAGCAGTGA AGGCCGTTCT GTGGGTATCA 
GCAGATGGAC TCAGAGTTGT GGATGAAAAA ACTAAGGACC TCATAGTTGA CCAGACAATA 
GAAAAAGTTT CTTTCTGCGC CCCCGATAGG AACTTTGACA GAGCCTTTTC TTACATATGT 
CGAGATGGCA CCACTCGGCG ATGGATCTGT CATTGCTTCA TGGCTGTCAA AGACACGGGG 
GAAAGACTGA GCCATGCCGT GGGCTGTGCT TTTGCAGCCT GTTTAGAGCG TAAACAGAAG 
CGGGAAAAGG AGTGTGGAGT CACTGCTACT TTCGATGCCA GTAGAACCAC TTTTACAAGA 
GAAGGATCAT TCCGTGTCAC AACTGCCACA GAACAAGCTG AAAGAGAGGA GATCATGAAA 
CAGTTGCAAG ATGCCAAGAA AGCTGAGACA GATAAGACCG TTGGTCCATC AGTGGCTCCT 
GGCAACAGTG CTCCATCGCC GTCCTCTCCC ACCTCCCCAA CTCTGGATCC CACTGCTTCT 
TTAGAGATGA ACAATCCTCA TGCTATCCCA CGCCGGCATG CACCAATTGA ACAGCTTGCT 
CGCCAAGGCT CTTTCCGGGG ATTTCCTGCT CTTAGCCAGA AGATGTCACC CTTTAAACGC 
CAGCTGTCCC TACGCATCAA TGAGCTGCCT TCCACTATGC AGAGGAAGAC TGATTTCCCA 
ATAAAAAACA CAGTGCCTGA GGTGGAAGGA GAGGCAGAAA GCATCAGCTC CCTGTGCTCC 
CAGATCACCA GTGCCTTCAG CACACCCTGT GAGGACCCCT TCTCCTCTGC CCCAATGACC 
AAACCAGTGA CATTAGTGGC ACCACAGTCT CCTGTGTTAC AAGGGACTGA GTGGGGTCAG 
TCTTCTGGTG CTGCCTCTCC AGGTCTCTTC CAGGCTGGTC ACAGACGCAC TCCCTCTGAG 
GCTGACCGTT GGTTAGAAGA AGTATCAAAA AGTGTGCGGG CCCAGCAGCC GCAGGCCTCA 
GCCGCCCCTC TGCAGCCAGT TCTGCAGCCT CCTCCGCCCG CTGCCATTGC CCCTCCAGCA 
CCTCCTTTCC AAGGACATGC ATTCCTCACT TCTCAGCCTG TGCCAGTGGG TGTGGTCCCA 
CCCCTACAAC CAGCCTTTGT CTCTACCCAG TCCTACCCTG TGGCCAATGG GATGCCCTAT 
CCAGCCTCTA ATGTGCCTGT AGTGGGCATC ACCCCATCCC AGATGGTAGC CAATGTGTTT 
GGCACTGCAG GCCATCCTCA GGCCACTCAT CCACATCAGT CCCCAAGCCT GGCCAAGCAG 
CAGACATTCC CTCAATATGA GACAAGTAGT GCTACCACCA GTCCCTTCTT TAAGCCTTCT 
GCTCAGCACC TCAATGGTTC TGCAGCTTTC AATGGTGTAG ACAATAGCGG GCTAGTCTCA 
GGAAACAGAC CTGCACAAGT CCCTCCAGGC ACCTGCCCAG TGGATCCTTT TGAGGCCCAG 
TGGGCTGCAC TAGAAAGCAA GCCCAAGCAG CGCACCAACC CCTCTCCTAC CAACCCTTTC 
TCCAGTGATG CACAGAAGGC ATTTGAAATA GAGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pattern of Notch2, Numb, and Cas8 Gene Expression in Relevant Structures of the Rat Brain during Formation of Spatial Memory.
Bulletin of experimental biology and medicine163(6)785-788(2017 Oct)
Gruden' MA,Storozheva ZI,Ratmirov AM,Sherstnev VV

Numb directs the subcellular localization of EAAT3 through binding the YxNxxF motif.
Journal of cell science129(16)3104-14(2016 08)
Su JF,Wei J,Li PS,Miao HH,Ma YC,Qu YX,Xu J,Qin J,Li BL,Song BL,Xu ZP,Luo J

Numb deficiency in cerebellar Purkinje cells impairs synaptic expression of metabotropic glutamate receptor and motor coordination.
Proceedings of the National Academy of Sciences of the United States of America112(50)15474-9(2015 Dec)
Zhou L,Yang D,Wang DJ,Xie YJ,Zhou JH,Zhou L,Huang H,Han S,Shao CY,Li HS,Zhu JJ,Qiu MS,De Zeeuw CI,Shen Y

Numb induces e-cadherin adhesion dissolution, cytoskeleton reorganization, and migration in tubular epithelial cells contributing to renal fibrosis.
Current molecular medicine15(4)368-79(2015)
Ding X,Ma M,Teng J,Shao F,Teng RK,Zhou S,Zhang Y,Wu E,Wang X

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Numb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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