Tas1r1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tas1r1
Entrez Gene ID	29407
Full Name	taste 1 receptor member 1
Synonyms	Gpr70
General protein information	Preferred Names taste 1 receptor member 1 Names taste receptor type 1 member 1 G-protein coupled receptor 70 taste receptor, type 1, member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	G-protein coupled receptor that is localized to the taste pore of taste buds and taste receptor cells; may act as a taste receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764341.1	XP_008762563.1	taste receptor type 1 member 1 isoform X1
NM_053305.1	NP_445757.1	taste receptor type 1 member 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TAS1R1	NP_619642.2
Mus musculus (house mouse)	Tas1r1	NP_114073.1
Rattus norvegicus (Norway rat)	Tas1r1	NP_445757.1
	TAS1R1	XP_001116977.1
Gallus gallus (chicken)	TAS1R1	XP_425734.3
Danio rerio (zebrafish)	tas1r1	NP_001034614.2
Pan troglodytes (chimpanzee)	TAS1R1	XP_525169.1
Bos taurus (cattle)	TAS1R1	XP_002694212.1

Gene Ontology
Bibliography

Related articles in PubMed

Human receptors for sweet and umami taste.
Li X, Staszewski L, Xu H, Durick K, Zoller M, Adler E
Proceedings of the National Academy of Sciences of the United States of America99(7)4692-6(2002 Apr)

Putative mammalian taste receptors: a class of taste-specific GPCRs with distinct topographic selectivity.
Hoon MA, Adler E, Lindemeier J, Battey JF, Ryba NJ, Zuker CS
Cell96(4)541-51(1999 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tas1r1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tas1r1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09222	NM_053305.1 Latest version!	Rattus norvegicus taste 1 receptor member 1 (Tas1r1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
ORa43264	XM_008764341.1 Latest version!	Rattus norvegicus taste 1 receptor member 1 (Tas1r1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tas1r1 ( NM_053305.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445757.1 Rattus norvegicus taste 1 receptor member 1 (Tas1r1), mRNA.

Price & Availability↑

CloneID	ORa09222
Clone ID Related Accession (Same CDS sequence)	NM_053305.1
Accession Version	NM_053305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	taste receptor type 1 member 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF127389.1 and CR460546.1. On or before Oct 28, 2007 this sequence version replaced XM_342986.2, XM_001076331.1. ##Evidence-Data-START## Transcript exon combination :: AF127389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642745 [ECO:0000348] ##Evidence-Data-END##

ATGCTCTTCT GGGCTGCTCA CCTGCTGCTC AGCCTGCAGT TGGTCTACTG CTGGGCTTTC 
AGCTGCCAAA GGACAGAGTC CTCTCCAGGC TTCAGCCTTC CTGGGGACTT CCTCCTTGCA 
GGTCTGTTCT CCCTCCATGG TGACTGTCTG CAGGTGAGAC ACAGACCTCT GGTGACAAGT 
TGTGACAGGC CCGACAGCTT CAACGGCCAT GGCTACCACC TCTTCCAAGC CATGCGGTTC 
ACTGTTGAGG AGATAAACAA CTCCTCGGCC CTGCTTCCCA ACATCACCCT GGGGTATGAG 
CTGTACGACG TGTGCTCAGA ATCTGCCAAT GTGTATGCCA CCCTGAGGGT GCTTGCCCTG 
CAAGGGCCCC GCCACATAGA GATACAGAAA GACCTTCGCA ACCACTCCTC CAAGGTGGTG 
GCCTTCATCG GGCCTGACAA CACTGACCAC GCTGTCACTA CCGCTGCCTT GCTGGGTCCT 
TTCCTGATGC CCCTGGTCAG CTATGAGGCA AGCAGCGTGG TACTCAGTGC CAAGCGCAAG 
TTCCCGTCTT TCCTTCGTAC CGTCCCCAGT GACCGGCACC AGGTGGAGGT CATGGTGCAG 
CTGCTGCAGA GTTTTGGGTG GGTGTGGATC TCGCTCATTG GCAGCTACGG TGATTACGGG 
CAGCTGGGTG TGCAGGCGCT GGAGGAGCTG GCCGTGCCCC GGGGCATCTG CGTCGCCTTC 
AAGGACATCG TGCCTTTCTC TGCCCGGGTG GGTGACCCGA GGATGCAGAG CATGATGCAG 
CATCTGGCTC AGGCCAGGAC CACCGTGGTT GTGGTCTTCT CTAACCGGCA CCTGGCTAGA 
GTGTTCTTCA GGTCCGTGGT GCTGGCCAAC CTGACTGGCA AAGTGTGGGT CGCCTCAGAA 
GACTGGGCCA TCTCCACGTA CATCACCAGC GTGACTGGGA TCCAAGGCAT TGGGACGGTG 
CTCGGTGTGG CCGTCCAGCA GAGACAAGTC CCTGGGCTGA AGGAGTTTGA GGAGTCTTAT 
GTCAGGGCTG TAACAGCTGC TCCCAGCGCT TGCCCGGAGG GGTCCTGGTG CAGCACTAAC 
CAGCTGTGCC GGGAGTGCCA CACGTTCACG ACTCGTAACA TGCCCACGCT TGGAGCCTTC 
TCCATGAGTG CCGCCTACAG AGTGTATGAG GCTGTGTACG CTGTGGCCCA CGGCCTCCAC 
CAGCTCCTGG GATGTACTTC TGAGATCTGT TCCAGAGGCC CAGTCTACCC CTGGCAGCTT 
CTTCAGCAGA TCTACAAGGT GAATTTTCTT CTACATGAGA ATACTGTGGC ATTTGATGAC 
AACGGGGACA CTCTAGGTTA CTACGACATC ATCGCCTGGG ACTGGAATGG ACCTGAATGG 
ACCTTTGAGA TCATTGGCTC TGCCTCACTG TCTCCAGTTC ATCTGGACAT AAATAAGACA 
AAAATCCAGT GGCACGGGAA GAACAATCAG GTGCCTGTGT CAGTGTGTAC CACGGACTGT 
CTGGCAGGGC ACCACAGGGT GGTTGTGGGT TCCCACCACT GCTGCTTTGA GTGTGTGCCC 
TGCGAAGCTG GGACCTTTCT CAACATGAGT GAGCTTCACA TCTGCCAGCC TTGTGGAACA 
GAAGAATGGG CACCCAAGGA GAGCACTACT TGCTTCCCAC GCACGGTGGA GTTCTTGGCT 
TGGCATGAAC CCATCTCTTT GGTGCTAATA GCAGCTAACA CGCTATTGCT GCTGCTGCTG 
GTTGGGACTG CTGGCCTGTT TGCCTGGCAT TTTCACACAC CTGTAGTGAG GTCAGCTGGG 
GGTAGGCTGT GCTTCCTCAT GCTGGGTTCC CTGGTGGCCG GAAGTTGCAG CTTCTATAGC 
TTCTTCGGGG AGCCCACGGT GCCCGCGTGC TTGCTGCGTC AGCCCCTCTT TTCTCTCGGG 
TTTGCCATCT TCCTCTCCTG CCTGACAATC CGCTCCTTCC AACTGGTCAT CATCTTCAAG 
TTTTCTACCA AGGTGCCCAC ATTCTACCGT ACCTGGGCCC AAAACCATGG TGCAGGTCTA 
TTCGTCATTG TCAGCTCCAC GGTCCATTTG CTCATCTGTC TCACATGGCT TGTAATGTGG 
ACCCCACGAC CCACCAGGGA ATACCAGCGC TTCCCCCATC TGGTGATTCT CGAGTGCACA 
GAGGTCAACT CTGTAGGCTT CCTGTTGGCT TTCACCCACA ACATTCTCCT CTCCATCAGT 
ACCTTCGTCT GCAGCTACCT GGGTAAGGAA CTGCCAGAGA ACTATAATGA AGCCAAATGT 
GTCACCTTCA GCCTGCTCCT CAACTTCGTA TCCTGGATCG CCTTCTTCAC CATGGCCAGC 
ATTTACCAGG GCAGCTACCT GCCTGCGGTC AATGTGCTGG CAGGGCTGAC CACACTGAGC 
GGCGGCTTCA GCGGTTACTT CCTCCCCAAG TGCTATGTGA TTCTCTGCCG TCCAGAACTC 
AACAATACAG AACACTTTCA GGCCTCCATC CAGGACTACA CGAGGCGCTG CGGCACTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445757.1
CDS	1..2523
Translation	MLFWAAHLLLSLQLVYCWAFSCQRTESSPGFSLPGDFLLAGLFSLHGDCLQVRHRPLVTSCDRPDSFNGHGYHLFQAMRFTVEEINNSSALLPNITLGYELYDVCSESANVYATLRVLALQGPRHIEIQKDLRNHSSKVVAFIGPDNTDHAVTTAALLGPFLMPLVSYEASSVVLSAKRKFPSFLRTVPSDRHQVEVMVQLLQSFGWVWISLIGSYGDYGQLGVQALEELAVPRGICVAFKDIVPFSARVGDPRMQSMMQHLAQARTTVVVVFSNRHLARVFFRSVVLANLTGKVWVASEDWAISTYITSVTGIQGIGTVLGVAVQQRQVPGLKEFEESYVRAVTAAPSACPEGSWCSTNQLCRECHTFTTRNMPTLGAFSMSAAYRVYEAVYAVAHGLHQLLGCTSEICSRGPVYPWQLLQQIYKVNFLLHENTVAFDDNGDTLGYYDIIAWDWNGPEWTFEIIGSASLSPVHLDINKTKIQWHGKNNQVPVSVCTTDCLAGHHRVVVGSHHCCFECVPCEAGTFLNMSELHICQPCGTEEWAPKESTTCFPRTVEFLAWHEPISLVLIAANTLLLLLLVGTAGLFAWHFHTPVVRSAGGRLCFLMLGSLVAGSCSFYSFFGEPTVPACLLRQPLFSLGFAIFLSCLTIRSFQLVIIFKFSTKVPTFYRTWAQNHGAGLFVIVSSTVHLLICLTWLVMWTPRPTREYQRFPHLVILECTEVNSVGFLLAFTHNILLSISTFVCSYLGKELPENYNEAKCVTFSLLLNFVSWIAFFTMASIYQGSYLPAVNVLAGLTTLSGGFSGYFLPKCYVILCRPELNNTEHFQASIQDYTRRCGTT

Target ORF information:

RefSeq Version	NM_053305.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus taste 1 receptor member 1 (Tas1r1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053305.1

ORF Insert Sequence:

ATGCTCTTCT GGGCTGCTCA CCTGCTGCTC AGCCTGCAGT TGGTCTACTG CTGGGCTTTC 
AGCTGCCAAA GGACAGAGTC CTCTCCAGGC TTCAGCCTTC CTGGGGACTT CCTCCTTGCA 
GGTCTGTTCT CCCTCCATGG TGACTGTCTG CAGGTGAGAC ACAGACCTCT GGTGACAAGT 
TGTGACAGGC CCGACAGCTT CAACGGCCAT GGCTACCACC TCTTCCAAGC CATGCGGTTC 
ACTGTTGAGG AGATAAACAA CTCCTCGGCC CTGCTTCCCA ACATCACCCT GGGGTATGAG 
CTGTACGACG TGTGCTCAGA ATCTGCCAAT GTGTATGCCA CCCTGAGGGT GCTTGCCCTG 
CAAGGGCCCC GCCACATAGA GATACAGAAA GACCTTCGCA ACCACTCCTC CAAGGTGGTG 
GCCTTCATCG GGCCTGACAA CACTGACCAC GCTGTCACTA CCGCTGCCTT GCTGGGTCCT 
TTCCTGATGC CCCTGGTCAG CTATGAGGCA AGCAGCGTGG TACTCAGTGC CAAGCGCAAG 
TTCCCGTCTT TCCTTCGTAC CGTCCCCAGT GACCGGCACC AGGTGGAGGT CATGGTGCAG 
CTGCTGCAGA GTTTTGGGTG GGTGTGGATC TCGCTCATTG GCAGCTACGG TGATTACGGG 
CAGCTGGGTG TGCAGGCGCT GGAGGAGCTG GCCGTGCCCC GGGGCATCTG CGTCGCCTTC 
AAGGACATCG TGCCTTTCTC TGCCCGGGTG GGTGACCCGA GGATGCAGAG CATGATGCAG 
CATCTGGCTC AGGCCAGGAC CACCGTGGTT GTGGTCTTCT CTAACCGGCA CCTGGCTAGA 
GTGTTCTTCA GGTCCGTGGT GCTGGCCAAC CTGACTGGCA AAGTGTGGGT CGCCTCAGAA 
GACTGGGCCA TCTCCACGTA CATCACCAGC GTGACTGGGA TCCAAGGCAT TGGGACGGTG 
CTCGGTGTGG CCGTCCAGCA GAGACAAGTC CCTGGGCTGA AGGAGTTTGA GGAGTCTTAT 
GTCAGGGCTG TAACAGCTGC TCCCAGCGCT TGCCCGGAGG GGTCCTGGTG CAGCACTAAC 
CAGCTGTGCC GGGAGTGCCA CACGTTCACG ACTCGTAACA TGCCCACGCT TGGAGCCTTC 
TCCATGAGTG CCGCCTACAG AGTGTATGAG GCTGTGTACG CTGTGGCCCA CGGCCTCCAC 
CAGCTCCTGG GATGTACTTC TGAGATCTGT TCCAGAGGCC CAGTCTACCC CTGGCAGCTT 
CTTCAGCAGA TCTACAAGGT GAATTTTCTT CTACATGAGA ATACTGTGGC ATTTGATGAC 
AACGGGGACA CTCTAGGTTA CTACGACATC ATCGCCTGGG ACTGGAATGG ACCTGAATGG 
ACCTTTGAGA TCATTGGCTC TGCCTCACTG TCTCCAGTTC ATCTGGACAT AAATAAGACA 
AAAATCCAGT GGCACGGGAA GAACAATCAG GTGCCTGTGT CAGTGTGTAC CACGGACTGT 
CTGGCAGGGC ACCACAGGGT GGTTGTGGGT TCCCACCACT GCTGCTTTGA GTGTGTGCCC 
TGCGAAGCTG GGACCTTTCT CAACATGAGT GAGCTTCACA TCTGCCAGCC TTGTGGAACA 
GAAGAATGGG CACCCAAGGA GAGCACTACT TGCTTCCCAC GCACGGTGGA GTTCTTGGCT 
TGGCATGAAC CCATCTCTTT GGTGCTAATA GCAGCTAACA CGCTATTGCT GCTGCTGCTG 
GTTGGGACTG CTGGCCTGTT TGCCTGGCAT TTTCACACAC CTGTAGTGAG GTCAGCTGGG 
GGTAGGCTGT GCTTCCTCAT GCTGGGTTCC CTGGTGGCCG GAAGTTGCAG CTTCTATAGC 
TTCTTCGGGG AGCCCACGGT GCCCGCGTGC TTGCTGCGTC AGCCCCTCTT TTCTCTCGGG 
TTTGCCATCT TCCTCTCCTG CCTGACAATC CGCTCCTTCC AACTGGTCAT CATCTTCAAG 
TTTTCTACCA AGGTGCCCAC ATTCTACCGT ACCTGGGCCC AAAACCATGG TGCAGGTCTA 
TTCGTCATTG TCAGCTCCAC GGTCCATTTG CTCATCTGTC TCACATGGCT TGTAATGTGG 
ACCCCACGAC CCACCAGGGA ATACCAGCGC TTCCCCCATC TGGTGATTCT CGAGTGCACA 
GAGGTCAACT CTGTAGGCTT CCTGTTGGCT TTCACCCACA ACATTCTCCT CTCCATCAGT 
ACCTTCGTCT GCAGCTACCT GGGTAAGGAA CTGCCAGAGA ACTATAATGA AGCCAAATGT 
GTCACCTTCA GCCTGCTCCT CAACTTCGTA TCCTGGATCG CCTTCTTCAC CATGGCCAGC 
ATTTACCAGG GCAGCTACCT GCCTGCGGTC AATGTGCTGG CAGGGCTGAC CACACTGAGC 
GGCGGCTTCA GCGGTTACTT CCTCCCCAAG TGCTATGTGA TTCTCTGCCG TCCAGAACTC 
AACAATACAG AACACTTTCA GGCCTCCATC CAGGACTACA CGAGGCGCTG CGGCACTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tas1r1 ( XM_008764341.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762563.1 Rattus norvegicus taste 1 receptor member 1 (Tas1r1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43264
Clone ID Related Accession (Same CDS sequence)	XM_008764341.1
Accession Version	XM_008764341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	taste receptor type 1 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTTCA CTGTTGAGGA GATAAACAAC TCCTCGGCCC TGCTTCCCAA CATCACCCTG 
GGGTATGAGC TGTACGACGT GTGCTCAGAA TCTGCCAATG TGTATGCCAC CCTGAGGGTG 
CTTGCCCTGC AAGGGCCCCG CCACATAGAG ATACAGAAAG ACCTTCGCAA CCACTCCTCC 
AAGGTGGTGG CCTTCATCGG GCCTGACAAC ACTGACCACG CTGTCACTAC CGCTGCCTTG 
CTGGGTCCTT TCCTGATGCC CCTGGTCAGC TATGAGGCAA GCAGCGTGGT ACTCAGTGCC 
AAGCGCAAGT TCCCGTCTTT CCTTCGTACC GTCCCCAGTG ACCGGCACCA GGTGGAGGTC 
ATGGTGCAGC TGCTGCAGAG TTTTGGGTGG GTGTGGATCT CGCTCATTGG CAGCTACGGT 
GATTACGGGC AGCTGGGTGT GCAGGCGCTG GAGGAGCTGG CCGTGCCCCG GGGCATCTGC 
GTCGCCTTCA AGGACATCGT GCCTTTCTCT GCCCGGGTGG GTGACCCGAG GATGCAGAGC 
ATGATGCAGC ATCTGGCTCA GGCCAGGACC ACCGTGGTTG TGGTCTTCTC TAACCGGCAC 
CTGGCTAGAG TGTTCTTCAG GTCCGTGGTG CTGGCCAACC TGACTGGCAA AGTGTGGGTC 
GCCTCAGAAG ACTGGGCCAT CTCCACGTAC ATCACCAGCG TGACTGGGAT CCAAGGCATT 
GGGACGGTGC TCGGTGTGGC CGTCCAGCAG AGACAAGTCC CTGGGCTGAA GGAGTTTGAG 
GAGTCTTATG TCAGGGCTGT AACAGCTGCT CCCAGCGCTT GCCCGGAGGG GTCCTGGTGC 
AGCACTAACC AGCTGTGCCG GGAGTGCCAC ACGTTCACGA CTCGTAACAT GCCCACGCTT 
GGAGCCTTCT CCATGAGTGC CGCCTACAGA GTGTATGAGG CTGTGTACGC TGTGGCCCAC 
GGCCTCCACC AGCTCCTGGG ATGTACTTCT GAGATCTGTT CCAGAGGCCC AGTCTACCCC 
TGGCAGCTTC TTCAGCAGAT CTACAAGGTG AATTTTCTTC TACATGAGAA TACTGTGGCA 
TTTGATGACA ACGGGGACAC TCTAGGTTAC TACGACATCA TCGCCTGGGA CTGGAATGGA 
CCTGAATGGA CCTTTGAGAT CATTGGCTCT GCCTCACTGT CTCCAGTTCA TCTGGACATA 
AATAAGACAA AAATCCAGTG GCACGGGAAG AACAATCAGG TGCCTGTGTC AGTGTGTACC 
ACGGACTGTC TGGCAGGGCA CCACAGGGTG GTTGTGGGTT CCCACCACTG CTGCTTTGAG 
TGTGTGCCCT GCGAAGCTGG GACCTTTCTC AACATGAGTG AGCTTCACAT CTGCCAGCCT 
TGTGGAACAG AAGAATGGGC ACCCAAGGAG AGCACTACTT GCTTCCCACG CACGGTGGAG 
TTCTTGGCTT GGCATGAACC CATCTCTTTG GTGCTAATAG CAGCTAACAC GCTATTGCTG 
CTGCTGCTGG TTGGGACTGC TGGCCTGTTT GCCTGGCATT TTCACACACC TGTAGTGAGG 
TCAGCTGGGG GTAGGCTGTG CTTCCTCATG CTGGGTTCCC TGGTGGCCGG AAGTTGCAGC 
TTCTATAGCT TCTTCGGGGA GCCCACGGTG CCCGCGTGCT TGCTGCGTCA GCCCCTCTTT 
TCTCTCGGGT TTGCCATCTT CCTCTCCTGC CTGACAATCC GCTCCTTCCA ACTGGTCATC 
ATCTTCAAGT TTTCTACCAA GGTGCCCACA TTCTACCGTA CCTGGGCCCA AAACCATGGT 
GCAGGTCTAT TCGTCATTGT CAGCTCCACG GTCCATTTGC TCATCTGTCT CACATGGCTT 
GTAATGTGGA CCCCACGACC CACCAGGGAA TACCAGCGCT TCCCCCATCT GGTGATTCTC 
GAGTGCACAG AGGTCAACTC TGTAGGCTTC CTGTTGGCTT TCACCCACAA CATTCTCCTC 
TCCATCAGTA CCTTCGTCTG CAGCTACCTG GGTAAGGAAC TGCCAGAGAA CTATAATGAA 
GCCAAATGTG TCACCTTCAG CCTGCTCCTC AACTTCGTAT CCTGGATCGC CTTCTTCACC 
ATGGCCAGCA TTTACCAGGG CAGCTACCTG CCTGCGGTCA ATGTGCTGGC AGGGCTGACC 
ACACTGAGCG GCGGCTTCAG CGGTTACTTC CTCCCCAAGT GCTATGTGAT TCTCTGCCGT 
CCAGAACTCA ACAATACAGA ACACTTTCAG GCCTCCATCC AGGACTACAC GAGGCGCTGC 
GGCACTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762563.1
CDS	92..2383
Translation	MRFTVEEINNSSALLPNITLGYELYDVCSESANVYATLRVLALQGPRHIEIQKDLRNHSSKVVAFIGPDNTDHAVTTAALLGPFLMPLVSYEASSVVLSAKRKFPSFLRTVPSDRHQVEVMVQLLQSFGWVWISLIGSYGDYGQLGVQALEELAVPRGICVAFKDIVPFSARVGDPRMQSMMQHLAQARTTVVVVFSNRHLARVFFRSVVLANLTGKVWVASEDWAISTYITSVTGIQGIGTVLGVAVQQRQVPGLKEFEESYVRAVTAAPSACPEGSWCSTNQLCRECHTFTTRNMPTLGAFSMSAAYRVYEAVYAVAHGLHQLLGCTSEICSRGPVYPWQLLQQIYKVNFLLHENTVAFDDNGDTLGYYDIIAWDWNGPEWTFEIIGSASLSPVHLDINKTKIQWHGKNNQVPVSVCTTDCLAGHHRVVVGSHHCCFECVPCEAGTFLNMSELHICQPCGTEEWAPKESTTCFPRTVEFLAWHEPISLVLIAANTLLLLLLVGTAGLFAWHFHTPVVRSAGGRLCFLMLGSLVAGSCSFYSFFGEPTVPACLLRQPLFSLGFAIFLSCLTIRSFQLVIIFKFSTKVPTFYRTWAQNHGAGLFVIVSSTVHLLICLTWLVMWTPRPTREYQRFPHLVILECTEVNSVGFLLAFTHNILLSISTFVCSYLGKELPENYNEAKCVTFSLLLNFVSWIAFFTMASIYQGSYLPAVNVLAGLTTLSGGFSGYFLPKCYVILCRPELNNTEHFQASIQDYTRRCGTT

Target ORF information:

RefSeq Version	XM_008764341.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus taste 1 receptor member 1 (Tas1r1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764341.1

ORF Insert Sequence:

ATGCGGTTCA CTGTTGAGGA GATAAACAAC TCCTCGGCCC TGCTTCCCAA CATCACCCTG 
GGGTATGAGC TGTACGACGT GTGCTCAGAA TCTGCCAATG TGTATGCCAC CCTGAGGGTG 
CTTGCCCTGC AAGGGCCCCG CCACATAGAG ATACAGAAAG ACCTTCGCAA CCACTCCTCC 
AAGGTGGTGG CCTTCATCGG GCCTGACAAC ACTGACCACG CTGTCACTAC CGCTGCCTTG 
CTGGGTCCTT TCCTGATGCC CCTGGTCAGC TATGAGGCAA GCAGCGTGGT ACTCAGTGCC 
AAGCGCAAGT TCCCGTCTTT CCTTCGTACC GTCCCCAGTG ACCGGCACCA GGTGGAGGTC 
ATGGTGCAGC TGCTGCAGAG TTTTGGGTGG GTGTGGATCT CGCTCATTGG CAGCTACGGT 
GATTACGGGC AGCTGGGTGT GCAGGCGCTG GAGGAGCTGG CCGTGCCCCG GGGCATCTGC 
GTCGCCTTCA AGGACATCGT GCCTTTCTCT GCCCGGGTGG GTGACCCGAG GATGCAGAGC 
ATGATGCAGC ATCTGGCTCA GGCCAGGACC ACCGTGGTTG TGGTCTTCTC TAACCGGCAC 
CTGGCTAGAG TGTTCTTCAG GTCCGTGGTG CTGGCCAACC TGACTGGCAA AGTGTGGGTC 
GCCTCAGAAG ACTGGGCCAT CTCCACGTAC ATCACCAGCG TGACTGGGAT CCAAGGCATT 
GGGACGGTGC TCGGTGTGGC CGTCCAGCAG AGACAAGTCC CTGGGCTGAA GGAGTTTGAG 
GAGTCTTATG TCAGGGCTGT AACAGCTGCT CCCAGCGCTT GCCCGGAGGG GTCCTGGTGC 
AGCACTAACC AGCTGTGCCG GGAGTGCCAC ACGTTCACGA CTCGTAACAT GCCCACGCTT 
GGAGCCTTCT CCATGAGTGC CGCCTACAGA GTGTATGAGG CTGTGTACGC TGTGGCCCAC 
GGCCTCCACC AGCTCCTGGG ATGTACTTCT GAGATCTGTT CCAGAGGCCC AGTCTACCCC 
TGGCAGCTTC TTCAGCAGAT CTACAAGGTG AATTTTCTTC TACATGAGAA TACTGTGGCA 
TTTGATGACA ACGGGGACAC TCTAGGTTAC TACGACATCA TCGCCTGGGA CTGGAATGGA 
CCTGAATGGA CCTTTGAGAT CATTGGCTCT GCCTCACTGT CTCCAGTTCA TCTGGACATA 
AATAAGACAA AAATCCAGTG GCACGGGAAG AACAATCAGG TGCCTGTGTC AGTGTGTACC 
ACGGACTGTC TGGCAGGGCA CCACAGGGTG GTTGTGGGTT CCCACCACTG CTGCTTTGAG 
TGTGTGCCCT GCGAAGCTGG GACCTTTCTC AACATGAGTG AGCTTCACAT CTGCCAGCCT 
TGTGGAACAG AAGAATGGGC ACCCAAGGAG AGCACTACTT GCTTCCCACG CACGGTGGAG 
TTCTTGGCTT GGCATGAACC CATCTCTTTG GTGCTAATAG CAGCTAACAC GCTATTGCTG 
CTGCTGCTGG TTGGGACTGC TGGCCTGTTT GCCTGGCATT TTCACACACC TGTAGTGAGG 
TCAGCTGGGG GTAGGCTGTG CTTCCTCATG CTGGGTTCCC TGGTGGCCGG AAGTTGCAGC 
TTCTATAGCT TCTTCGGGGA GCCCACGGTG CCCGCGTGCT TGCTGCGTCA GCCCCTCTTT 
TCTCTCGGGT TTGCCATCTT CCTCTCCTGC CTGACAATCC GCTCCTTCCA ACTGGTCATC 
ATCTTCAAGT TTTCTACCAA GGTGCCCACA TTCTACCGTA CCTGGGCCCA AAACCATGGT 
GCAGGTCTAT TCGTCATTGT CAGCTCCACG GTCCATTTGC TCATCTGTCT CACATGGCTT 
GTAATGTGGA CCCCACGACC CACCAGGGAA TACCAGCGCT TCCCCCATCT GGTGATTCTC 
GAGTGCACAG AGGTCAACTC TGTAGGCTTC CTGTTGGCTT TCACCCACAA CATTCTCCTC 
TCCATCAGTA CCTTCGTCTG CAGCTACCTG GGTAAGGAAC TGCCAGAGAA CTATAATGAA 
GCCAAATGTG TCACCTTCAG CCTGCTCCTC AACTTCGTAT CCTGGATCGC CTTCTTCACC 
ATGGCCAGCA TTTACCAGGG CAGCTACCTG CCTGCGGTCA ATGTGCTGGC AGGGCTGACC 
ACACTGAGCG GCGGCTTCAG CGGTTACTTC CTCCCCAAGT GCTATGTGAT TCTCTGCCGT 
CCAGAACTCA ACAATACAGA ACACTTTCAG GCCTCCATCC AGGACTACAC GAGGCGCTGC 
GGCACTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human receptors for sweet and umami taste.
Proceedings of the National Academy of Sciences of the United States of America99(7)4692-6(2002 Apr)
Li X,Staszewski L,Xu H,Durick K,Zoller M,Adler E

Putative mammalian taste receptors: a class of taste-specific GPCRs with distinct topographic selectivity.
Cell96(4)541-51(1999 Feb)
Hoon MA,Adler E,Lindemeier J,Battey JF,Ryba NJ,Zuker CS

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Tas1r1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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