TAS1R1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAS1R1
Entrez Gene ID	80835
Full Name	taste 1 receptor member 1
Synonyms	GM148,GPR70,T1R1,TR1
General protein information	Preferred Names taste 1 receptor member 1 Names taste receptor type 1 member 1 G-protein coupled receptor 70 seven transmembrane helix receptor taste receptor, type 1, member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.31
Summary	The protein encoded by this gene is a G protein-coupled receptor and is a component of the heterodimeric amino acid taste receptor T1R1+3. The T1R1+3 receptor responds to L-amino acids but not to D-enantiomers or other compounds. Most amino acids that are perceived as sweet activate T1R1+3, and this activation is strictly dependent on an intact T1R1+3 heterodimer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010].
Disorder MIM:	606225

mRNA and Protein(s)

mRNA	Protein	Name
NM_177540.3	NP_803884.1	taste receptor type 1 member 1 isoform c precursor
NM_138697.3	NP_619642.2	taste receptor type 1 member 1 isoform b precursor
NM_138697.4	NP_619642.2	taste receptor type 1 member 1 isoform b precursor
XM_011542203.2	XP_011540505.1	taste receptor type 1 member 1 isoform X2
XM_017002403.1	XP_016857892.1	taste receptor type 1 member 1 isoform X3
XM_011542206.2	XP_011540508.1	taste receptor type 1 member 1 isoform X4
NM_177540.2	NP_803884.1	taste receptor type 1 member 1 isoform c precursor
XM_017002402.1	XP_016857891.1	taste receptor type 1 member 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TAS1R1	NP_619642.2
Mus musculus (house mouse)	Tas1r1	NP_114073.1
Rattus norvegicus (Norway rat)	Tas1r1	NP_445757.1
	TAS1R1	XP_001116977.1
Gallus gallus (chicken)	TAS1R1	XP_425734.3
Danio rerio (zebrafish)	tas1r1	NP_001034614.2
Pan troglodytes (chimpanzee)	TAS1R1	XP_525169.1
Bos taurus (cattle)	TAS1R1	XP_002694212.1

Gene Ontology
Bibliography

Related articles in PubMed

Variations in TAS1R taste receptor gene family modify food intake and gastric cancer risk in a Korean population.
Choi JH, Lee J, Choi IJ, Kim YW, Ryu KW, Kim J
Molecular nutrition & food research60(11)2433-2445(2016 Nov)

Two distinct determinants of ligand specificity in T1R1/T1R3 (the umami taste receptor).
Toda Y, Nakagita T, Hayakawa T, Okada S, Narukawa M, Imai H, Ishimaru Y, Misaka T
The Journal of biological chemistry288(52)36863-77(2013 Dec)

Do polymorphisms in the TAS1R1 gene contribute to broader differences in human taste intensity?
Rawal S, Hayes JE, Wallace MR, Bartoshuk LM, Duffy VB
Chemical senses38(8)719-28(2013 Oct)

Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca?? and cAMP concentrations in spermatozoa.
Meyer D, Voigt A, Widmayer P, Borth H, Huebner S, Breit A, Marschall S, de Angelis MH, Boehm U, Meyerhof W, Gudermann T, Boekhoff I
PloS one7(2)e32354(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic variants in TAS1R were associated with dietary consumption, which may be associated with gastric cancer risk
Title: Variations in TAS1R taste receptor gene family modify food intake and gastric cancer risk in a Korean population.

A complex molecular mechanism involving changes in the properties of both the orthosteric and non-orthosteric sites of T1R1 underlies the determination of ligand specificity in mammalian T1R1/T1R3.
Title: Two distinct determinants of ligand specificity in T1R1/T1R3 (the umami taste receptor).

The study provides human psychophysical evidence that TAS1R1 polymorphisms associate with modest differences in overall taste intensity, which were independent from other well-studied markers of variation in oral sensation.
Title: Do polymorphisms in the TAS1R1 gene contribute to broader differences in human taste intensity?

Data show that that Tas1r1 and Tas1r3 are expressed in murine and human spermatozoa.
Title: Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca?? and cAMP concentrations in spermatozoa.

amino acid substitutions (A110V and R507Q) in the N-terminal ligand-binding domain of T1R1 severely impair receptor functions in vitro
Title: Human genetic polymorphisms in T1R1 and T1R3 taste receptor subunits affect their function.

The following TAS1R1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAS1R1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15305	NM_138697.4 Latest version!	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu72850	XM_011542203.2 Latest version!	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu14934	NM_177540.3 Latest version!	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OHu72852	XM_011542206.2 Latest version!	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu90569	XM_017002402.1 Latest version!	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu90570	XM_017002403.1 Latest version!	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu15305	NM_138697.3	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14934	NM_177540.2	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAS1R1 ( NM_138697.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_619642.2 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15305
Clone ID Related Accession (Same CDS sequence)	NM_138697.3 , NM_138697.4
Accession Version	NM_138697.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC136515.1, BC136516.1, AL591866.13 and AI742401.1. On Jun 5, 2010 this sequence version replaced NM_138697.2. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136515.1, BC136516.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGAT TAGCTATGCG GCCAGCAGCG AGACGCTCAG CGTGAAGCGG 
CAGTATCCCT CTTTCCTGCG CACCATCCCC AATGACAAGT ACCAGGTGGA GACCATGGTG 
CTGCTGCTGC AGAAGTTCGG GTGGACCTGG ATCTCTCTGG TTGGCAGCAG TGACGACTAT 
GGGCAGCTAG GGGTGCAGGC ACTGGAGAAC CAGGCCACTG GTCAGGGGAT CTGCATTGCT 
TTCAAGGACA TCATGCCCTT CTCTGCCCAG GTGGGCGATG AGAGGATGCA GTGCCTCATG 
CGCCACCTGG CCCAGGCCGG GGCCACCGTC GTGGTTGTTT TTTCCAGCCG GCAGTTGGCC 
AGGGTGTTTT TCGAGTCCGT GGTGCTGACC AACCTGACTG GCAAGGTGTG GGTCGCCTCA 
GAAGCCTGGG CCCTCTCCAG GCACATCACT GGGGTGCCCG GGATCCAGCG CATTGGGATG 
GTGCTGGGCG TGGCCATCCA GAAGAGGGCT GTCCCTGGCC TGAAGGCGTT TGAAGAAGCC 
TATGCCCGGG CAGACAAGAA GGCCCCTAGG CCTTGCCACA AGGGCTCCTG GTGCAGCAGC 
AATCAGCTCT GCAGAGAATG CCAAGCTTTC ATGGCACACA CGATGCCCAA GCTCAAAGCC 
TTCTCCATGA GTTCTGCCTA CAACGCATAC CGGGCTGTGT ATGCGGTGGC CCATGGCCTC 
CACCAGCTCC TGGGCTGTGC CTCTGGAGCT TGTTCCAGGG GCCGAGTCTA CCCCTGGCAG 
CTTTTGGAGC AGATCCACAA GGTGCATTTC CTTCTACACA AGGACACTGT GGCGTTTAAT 
GACAACAGAG ATCCCCTCAG TAGCTATAAC ATAATTGCCT GGGACTGGAA TGGACCCAAG 
TGGACCTTCA CGGTCCTCGG TTCCTCCACA TGGTCTCCAG TTCAGCTAAA CATAAATGAG 
ACCAAAATCC AGTGGCACGG AAAGGACAAC CAGGTGCCTA AGTCTGTGTG TTCCAGCGAC 
TGTCTTGAAG GGCACCAGCG AGTGGTTACG GGTTTCCATC ACTGCTGCTT TGAGTGTGTG 
CCCTGTGGGG CTGGGACCTT CCTCAACAAG AGTGACCTCT ACAGATGCCA GCCTTGTGGG 
AAAGAAGAGT GGGCACCTGA GGGAAGCCAG ACCTGCTTCC CGCGCACTGT GGTGTTTTTG 
GCTTTGCGTG AGCACACCTC TTGGGTGCTG CTGGCAGCTA ACACGCTGCT GCTGCTGCTG 
CTGCTTGGGA CTGCTGGCCT GTTTGCCTGG CACCTAGACA CCCCTGTGGT GAGGTCAGCA 
GGGGGCCGCC TGTGCTTTCT TATGCTGGGC TCCCTGGCAG CAGGTAGTGG CAGCCTCTAT 
GGCTTCTTTG GGGAACCCAC AAGGCCTGCG TGCTTGCTAC GCCAGGCCCT CTTTGCCCTT 
GGTTTCACCA TCTTCCTGTC CTGCCTGACA GTTCGCTCAT TCCAACTAAT CATCATCTTC 
AAGTTTTCCA CCAAGGTACC TACATTCTAC CACGCCTGGG TCCAAAACCA CGGTGCTGGC 
CTGTTTGTGA TGATCAGCTC AGCGGCCCAG CTGCTTATCT GTCTAACTTG GCTGGTGGTG 
TGGACCCCAC TGCCTGCTAG GGAATACCAG CGCTTCCCCC ATCTGGTGAT GCTTGAGTGC 
ACAGAGACCA ACTCCCTGGG CTTCATACTG GCCTTCCTCT ACAATGGCCT CCTCTCCATC 
AGTGCCTTTG CCTGCAGCTA CCTGGGTAAG GACTTGCCAG AGAACTACAA CGAGGCCAAA 
TGTGTCACCT TCAGCCTGCT CTTCAACTTC GTGTCCTGGA TCGCCTTCTT CACCACGGCC 
AGCGTCTACG ACGGCAAGTA CCTGCCTGCG GCCAACATGA TGGCTGGGCT GAGCAGCCTG 
AGCAGCGGCT TCGGTGGGTA TTTTCTGCCT AAGTGCTACG TGATCCTCTG CCGCCCAGAC 
CTCAACAGCA CAGAGCACTT CCAGGCCTCC ATTCAGGACT ACACGAGGCG CTGCGGCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619642.2
CDS	97..2622
Translation	MLLCTARLVGLQLLISCCWAFACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	NM_138697.3
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138697.3

ORF Insert Sequence:

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGAT TAGCTATGCG GCCAGCAGCG AGACGCTCAG CGTGAAGCGG 
CAGTATCCCT CTTTCCTGCG CACCATCCCC AATGACAAGT ACCAGGTGGA GACCATGGTG 
CTGCTGCTGC AGAAGTTCGG GTGGACCTGG ATCTCTCTGG TTGGCAGCAG TGACGACTAT 
GGGCAGCTAG GGGTGCAGGC ACTGGAGAAC CAGGCCACTG GTCAGGGGAT CTGCATTGCT 
TTCAAGGACA TCATGCCCTT CTCTGCCCAG GTGGGCGATG AGAGGATGCA GTGCCTCATG 
CGCCACCTGG CCCAGGCCGG GGCCACCGTC GTGGTTGTTT TTTCCAGCCG GCAGTTGGCC 
AGGGTGTTTT TCGAGTCCGT GGTGCTGACC AACCTGACTG GCAAGGTGTG GGTCGCCTCA 
GAAGCCTGGG CCCTCTCCAG GCACATCACT GGGGTGCCCG GGATCCAGCG CATTGGGATG 
GTGCTGGGCG TGGCCATCCA GAAGAGGGCT GTCCCTGGCC TGAAGGCGTT TGAAGAAGCC 
TATGCCCGGG CAGACAAGAA GGCCCCTAGG CCTTGCCACA AGGGCTCCTG GTGCAGCAGC 
AATCAGCTCT GCAGAGAATG CCAAGCTTTC ATGGCACACA CGATGCCCAA GCTCAAAGCC 
TTCTCCATGA GTTCTGCCTA CAACGCATAC CGGGCTGTGT ATGCGGTGGC CCATGGCCTC 
CACCAGCTCC TGGGCTGTGC CTCTGGAGCT TGTTCCAGGG GCCGAGTCTA CCCCTGGCAG 
CTTTTGGAGC AGATCCACAA GGTGCATTTC CTTCTACACA AGGACACTGT GGCGTTTAAT 
GACAACAGAG ATCCCCTCAG TAGCTATAAC ATAATTGCCT GGGACTGGAA TGGACCCAAG 
TGGACCTTCA CGGTCCTCGG TTCCTCCACA TGGTCTCCAG TTCAGCTAAA CATAAATGAG 
ACCAAAATCC AGTGGCACGG AAAGGACAAC CAGGTGCCTA AGTCTGTGTG TTCCAGCGAC 
TGTCTTGAAG GGCACCAGCG AGTGGTTACG GGTTTCCATC ACTGCTGCTT TGAGTGTGTG 
CCCTGTGGGG CTGGGACCTT CCTCAACAAG AGTGACCTCT ACAGATGCCA GCCTTGTGGG 
AAAGAAGAGT GGGCACCTGA GGGAAGCCAG ACCTGCTTCC CGCGCACTGT GGTGTTTTTG 
GCTTTGCGTG AGCACACCTC TTGGGTGCTG CTGGCAGCTA ACACGCTGCT GCTGCTGCTG 
CTGCTTGGGA CTGCTGGCCT GTTTGCCTGG CACCTAGACA CCCCTGTGGT GAGGTCAGCA 
GGGGGCCGCC TGTGCTTTCT TATGCTGGGC TCCCTGGCAG CAGGTAGTGG CAGCCTCTAT 
GGCTTCTTTG GGGAACCCAC AAGGCCTGCG TGCTTGCTAC GCCAGGCCCT CTTTGCCCTT 
GGTTTCACCA TCTTCCTGTC CTGCCTGACA GTTCGCTCAT TCCAACTAAT CATCATCTTC 
AAGTTTTCCA CCAAGGTACC TACATTCTAC CACGCCTGGG TCCAAAACCA CGGTGCTGGC 
CTGTTTGTGA TGATCAGCTC AGCGGCCCAG CTGCTTATCT GTCTAACTTG GCTGGTGGTG 
TGGACCCCAC TGCCTGCTAG GGAATACCAG CGCTTCCCCC ATCTGGTGAT GCTTGAGTGC 
ACAGAGACCA ACTCCCTGGG CTTCATACTG GCCTTCCTCT ACAATGGCCT CCTCTCCATC 
AGTGCCTTTG CCTGCAGCTA CCTGGGTAAG GACTTGCCAG AGAACTACAA CGAGGCCAAA 
TGTGTCACCT TCAGCCTGCT CTTCAACTTC GTGTCCTGGA TCGCCTTCTT CACCACGGCC 
AGCGTCTACG ACGGCAAGTA CCTGCCTGCG GCCAACATGA TGGCTGGGCT GAGCAGCCTG 
AGCAGCGGCT TCGGTGGGTA TTTTCTGCCT AAGTGCTACG TGATCCTCTG CCGCCCAGAC 
CTCAACAGCA CAGAGCACTT CCAGGCCTCC ATTCAGGACT ACACGAGGCG CTGCGGCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( NM_138697.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_619642.2 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15305
Clone ID Related Accession (Same CDS sequence)	NM_138697.3 , NM_138697.4
Accession Version	NM_138697.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC136515.1, BC136516.1, AL591866.13 and AI742401.1. On Nov 23, 2018 this sequence version replaced NM_138697.3. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136515.1, AK315805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333172.11/ ENSP00000331867.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGAT TAGCTATGCG GCCAGCAGCG AGACGCTCAG CGTGAAGCGG 
CAGTATCCCT CTTTCCTGCG CACCATCCCC AATGACAAGT ACCAGGTGGA GACCATGGTG 
CTGCTGCTGC AGAAGTTCGG GTGGACCTGG ATCTCTCTGG TTGGCAGCAG TGACGACTAT 
GGGCAGCTAG GGGTGCAGGC ACTGGAGAAC CAGGCCACTG GTCAGGGGAT CTGCATTGCT 
TTCAAGGACA TCATGCCCTT CTCTGCCCAG GTGGGCGATG AGAGGATGCA GTGCCTCATG 
CGCCACCTGG CCCAGGCCGG GGCCACCGTC GTGGTTGTTT TTTCCAGCCG GCAGTTGGCC 
AGGGTGTTTT TCGAGTCCGT GGTGCTGACC AACCTGACTG GCAAGGTGTG GGTCGCCTCA 
GAAGCCTGGG CCCTCTCCAG GCACATCACT GGGGTGCCCG GGATCCAGCG CATTGGGATG 
GTGCTGGGCG TGGCCATCCA GAAGAGGGCT GTCCCTGGCC TGAAGGCGTT TGAAGAAGCC 
TATGCCCGGG CAGACAAGAA GGCCCCTAGG CCTTGCCACA AGGGCTCCTG GTGCAGCAGC 
AATCAGCTCT GCAGAGAATG CCAAGCTTTC ATGGCACACA CGATGCCCAA GCTCAAAGCC 
TTCTCCATGA GTTCTGCCTA CAACGCATAC CGGGCTGTGT ATGCGGTGGC CCATGGCCTC 
CACCAGCTCC TGGGCTGTGC CTCTGGAGCT TGTTCCAGGG GCCGAGTCTA CCCCTGGCAG 
CTTTTGGAGC AGATCCACAA GGTGCATTTC CTTCTACACA AGGACACTGT GGCGTTTAAT 
GACAACAGAG ATCCCCTCAG TAGCTATAAC ATAATTGCCT GGGACTGGAA TGGACCCAAG 
TGGACCTTCA CGGTCCTCGG TTCCTCCACA TGGTCTCCAG TTCAGCTAAA CATAAATGAG 
ACCAAAATCC AGTGGCACGG AAAGGACAAC CAGGTGCCTA AGTCTGTGTG TTCCAGCGAC 
TGTCTTGAAG GGCACCAGCG AGTGGTTACG GGTTTCCATC ACTGCTGCTT TGAGTGTGTG 
CCCTGTGGGG CTGGGACCTT CCTCAACAAG AGTGACCTCT ACAGATGCCA GCCTTGTGGG 
AAAGAAGAGT GGGCACCTGA GGGAAGCCAG ACCTGCTTCC CGCGCACTGT GGTGTTTTTG 
GCTTTGCGTG AGCACACCTC TTGGGTGCTG CTGGCAGCTA ACACGCTGCT GCTGCTGCTG 
CTGCTTGGGA CTGCTGGCCT GTTTGCCTGG CACCTAGACA CCCCTGTGGT GAGGTCAGCA 
GGGGGCCGCC TGTGCTTTCT TATGCTGGGC TCCCTGGCAG CAGGTAGTGG CAGCCTCTAT 
GGCTTCTTTG GGGAACCCAC AAGGCCTGCG TGCTTGCTAC GCCAGGCCCT CTTTGCCCTT 
GGTTTCACCA TCTTCCTGTC CTGCCTGACA GTTCGCTCAT TCCAACTAAT CATCATCTTC 
AAGTTTTCCA CCAAGGTACC TACATTCTAC CACGCCTGGG TCCAAAACCA CGGTGCTGGC 
CTGTTTGTGA TGATCAGCTC AGCGGCCCAG CTGCTTATCT GTCTAACTTG GCTGGTGGTG 
TGGACCCCAC TGCCTGCTAG GGAATACCAG CGCTTCCCCC ATCTGGTGAT GCTTGAGTGC 
ACAGAGACCA ACTCCCTGGG CTTCATACTG GCCTTCCTCT ACAATGGCCT CCTCTCCATC 
AGTGCCTTTG CCTGCAGCTA CCTGGGTAAG GACTTGCCAG AGAACTACAA CGAGGCCAAA 
TGTGTCACCT TCAGCCTGCT CTTCAACTTC GTGTCCTGGA TCGCCTTCTT CACCACGGCC 
AGCGTCTACG ACGGCAAGTA CCTGCCTGCG GCCAACATGA TGGCTGGGCT GAGCAGCCTG 
AGCAGCGGCT TCGGTGGGTA TTTTCTGCCT AAGTGCTACG TGATCCTCTG CCGCCCAGAC 
CTCAACAGCA CAGAGCACTT CCAGGCCTCC ATTCAGGACT ACACGAGGCG CTGCGGCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619642.2
CDS	68..2593
Translation	MLLCTARLVGLQLLISCCWAFACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	NM_138697.4
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138697.4

ORF Insert Sequence:

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGAT TAGCTATGCG GCCAGCAGCG AGACGCTCAG CGTGAAGCGG 
CAGTATCCCT CTTTCCTGCG CACCATCCCC AATGACAAGT ACCAGGTGGA GACCATGGTG 
CTGCTGCTGC AGAAGTTCGG GTGGACCTGG ATCTCTCTGG TTGGCAGCAG TGACGACTAT 
GGGCAGCTAG GGGTGCAGGC ACTGGAGAAC CAGGCCACTG GTCAGGGGAT CTGCATTGCT 
TTCAAGGACA TCATGCCCTT CTCTGCCCAG GTGGGCGATG AGAGGATGCA GTGCCTCATG 
CGCCACCTGG CCCAGGCCGG GGCCACCGTC GTGGTTGTTT TTTCCAGCCG GCAGTTGGCC 
AGGGTGTTTT TCGAGTCCGT GGTGCTGACC AACCTGACTG GCAAGGTGTG GGTCGCCTCA 
GAAGCCTGGG CCCTCTCCAG GCACATCACT GGGGTGCCCG GGATCCAGCG CATTGGGATG 
GTGCTGGGCG TGGCCATCCA GAAGAGGGCT GTCCCTGGCC TGAAGGCGTT TGAAGAAGCC 
TATGCCCGGG CAGACAAGAA GGCCCCTAGG CCTTGCCACA AGGGCTCCTG GTGCAGCAGC 
AATCAGCTCT GCAGAGAATG CCAAGCTTTC ATGGCACACA CGATGCCCAA GCTCAAAGCC 
TTCTCCATGA GTTCTGCCTA CAACGCATAC CGGGCTGTGT ATGCGGTGGC CCATGGCCTC 
CACCAGCTCC TGGGCTGTGC CTCTGGAGCT TGTTCCAGGG GCCGAGTCTA CCCCTGGCAG 
CTTTTGGAGC AGATCCACAA GGTGCATTTC CTTCTACACA AGGACACTGT GGCGTTTAAT 
GACAACAGAG ATCCCCTCAG TAGCTATAAC ATAATTGCCT GGGACTGGAA TGGACCCAAG 
TGGACCTTCA CGGTCCTCGG TTCCTCCACA TGGTCTCCAG TTCAGCTAAA CATAAATGAG 
ACCAAAATCC AGTGGCACGG AAAGGACAAC CAGGTGCCTA AGTCTGTGTG TTCCAGCGAC 
TGTCTTGAAG GGCACCAGCG AGTGGTTACG GGTTTCCATC ACTGCTGCTT TGAGTGTGTG 
CCCTGTGGGG CTGGGACCTT CCTCAACAAG AGTGACCTCT ACAGATGCCA GCCTTGTGGG 
AAAGAAGAGT GGGCACCTGA GGGAAGCCAG ACCTGCTTCC CGCGCACTGT GGTGTTTTTG 
GCTTTGCGTG AGCACACCTC TTGGGTGCTG CTGGCAGCTA ACACGCTGCT GCTGCTGCTG 
CTGCTTGGGA CTGCTGGCCT GTTTGCCTGG CACCTAGACA CCCCTGTGGT GAGGTCAGCA 
GGGGGCCGCC TGTGCTTTCT TATGCTGGGC TCCCTGGCAG CAGGTAGTGG CAGCCTCTAT 
GGCTTCTTTG GGGAACCCAC AAGGCCTGCG TGCTTGCTAC GCCAGGCCCT CTTTGCCCTT 
GGTTTCACCA TCTTCCTGTC CTGCCTGACA GTTCGCTCAT TCCAACTAAT CATCATCTTC 
AAGTTTTCCA CCAAGGTACC TACATTCTAC CACGCCTGGG TCCAAAACCA CGGTGCTGGC 
CTGTTTGTGA TGATCAGCTC AGCGGCCCAG CTGCTTATCT GTCTAACTTG GCTGGTGGTG 
TGGACCCCAC TGCCTGCTAG GGAATACCAG CGCTTCCCCC ATCTGGTGAT GCTTGAGTGC 
ACAGAGACCA ACTCCCTGGG CTTCATACTG GCCTTCCTCT ACAATGGCCT CCTCTCCATC 
AGTGCCTTTG CCTGCAGCTA CCTGGGTAAG GACTTGCCAG AGAACTACAA CGAGGCCAAA 
TGTGTCACCT TCAGCCTGCT CTTCAACTTC GTGTCCTGGA TCGCCTTCTT CACCACGGCC 
AGCGTCTACG ACGGCAAGTA CCTGCCTGCG GCCAACATGA TGGCTGGGCT GAGCAGCCTG 
AGCAGCGGCT TCGGTGGGTA TTTTCTGCCT AAGTGCTACG TGATCCTCTG CCGCCCAGAC 
CTCAACAGCA CAGAGCACTT CCAGGCCTCC ATTCAGGACT ACACGAGGCG CTGCGGCTCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( XM_011542203.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540505.1 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X2, mRNA.

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CloneID	OHu72850
Clone ID Related Accession (Same CDS sequence)	XM_011542203.2
Accession Version	XM_011542203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGATTAGC TATGCGGCCA GCAGCGAGAC GCTCAGCGTG 
AAGCGGCAGT ATCCCTCTTT CCTGCGCACC ATCCCCAATG ACAAGTACCA GGTGGAGACC 
ATGGTGCTGC TGCTGCAGAA GTTCGGGTGG ACCTGGATCT CTCTGGTTGG CAGCAGTGAC 
GACTATGGGC AGCTAGGGGT GCAGGCACTG GAGAACCAGG CCACTGGTCA GGGGATCTGC 
ATTGCTTTCA AGGACATCAT GCCCTTCTCT GCCCAGGTGG GCGATGAGAG GATGCAGTGC 
CTCATGCGCC ACCTGGCCCA GGCCGGGGCC ACCGTCGTGG TTGTTTTTTC CAGCCGGCAG 
TTGGCCAGGG TGTTTTTCGA GTCCGTGGTG CTGACCAACC TGACTGGCAA GGTGTGGGTC 
GCCTCAGAAG CCTGGGCCCT CTCCAGGCAC ATCACTGGGG TGCCCGGGAT CCAGCGCATT 
GGGATGGTGC TGGGCGTGGC CATCCAGAAG AGGGCTGTCC CTGGCCTGAA GGCGTTTGAA 
GAAGCCTATG CCCGGGCAGA CAAGAAGGCC CCTAGGCCTT GCCACAAGGG CTCCTGGTGC 
AGCAGCAATC AGCTCTGCAG AGAATGCCAA GCTTTCATGG CACACACGAT GCCCAAGCTC 
AAAGCCTTCT CCATGAGTTC TGCCTACAAC GCATACCGGG CTGTGTATGC GGTGGCCCAT 
GGCCTCCACC AGCTCCTGGG CTGTGCCTCT GGAGCTTGTT CCAGGGGCCG AGTCTACCCC 
TGGCAGCTTT TGGAGCAGAT CCACAAGGTG CATTTCCTTC TACACAAGGA CACTGTGGCG 
TTTAATGACA ACAGAGATCC CCTCAGTAGC TATAACATAA TTGCCTGGGA CTGGAATGGA 
CCCAAGTGGA CCTTCACGGT CCTCGGTTCC TCCACATGGT CTCCAGTTCA GCTAAACATA 
AATGAGACCA AAATCCAGTG GCACGGAAAG GACAACCAGG TGCCTAAGTC TGTGTGTTCC 
AGCGACTGTC TTGAAGGGCA CCAGCGAGTG GTTACGGGTT TCCATCACTG CTGCTTTGAG 
TGTGTGCCCT GTGGGGCTGG GACCTTCCTC AACAAGAGTG ACCTCTACAG ATGCCAGCCT 
TGTGGGAAAG AAGAGTGGGC ACCTGAGGGA AGCCAGACCT GCTTCCCGCG CACTGTGGTG 
TTTTTGGCTT TGCGTGAGCA CACCTCTTGG GTGCTGCTGG CAGCTAACAC GCTGCTGCTG 
CTGCTGCTGC TTGGGACTGC TGGCCTGTTT GCCTGGCACC TAGACACCCC TGTGGTGAGG 
TCAGCAGGGG GCCGCCTGTG CTTTCTTATG CTGGGCTCCC TGGCAGCAGG TAGTGGCAGC 
CTCTATGGCT TCTTTGGGGA ACCCACAAGG CCTGCGTGCT TGCTACGCCA GGCCCTCTTT 
GCCCTTGGTT TCACCATCTT CCTGTCCTGC CTGACAGTTC GCTCATTCCA ACTAATCATC 
ATCTTCAAGT TTTCCACCAA GGTACCTACA TTCTACCACG CCTGGGTCCA AAACCACGGT 
GCTGGCCTGT TTGTGATGAT CAGCTCAGCG GCCCAGCTGC TTATCTGTCT AACTTGGCTG 
GTGGTGTGGA CCCCACTGCC TGCTAGGGAA TACCAGCGCT TCCCCCATCT GGTGATGCTT 
GAGTGCACAG AGACCAACTC CCTGGGCTTC ATACTGGCCT TCCTCTACAA TGGCCTCCTC 
TCCATCAGTG CCTTTGCCTG CAGCTACCTG GGTAAGGACT TGCCAGAGAA CTACAACGAG 
GCCAAATGTG TCACCTTCAG CCTGCTCTTC AACTTCGTGT CCTGGATCGC CTTCTTCACC 
ACGGCCAGCG TCTACGACGG CAAGTACCTG CCTGCGGCCA ACATGATGGC TGGGCTGAGC 
AGCCTGAGCA GCGGCTTCGG TGGGTATTTT CTGCCTAAGT GCTACGTGAT CCTCTGCCGC 
CCAGACCTCA ACAGCACAGA GCACTTCCAG GCCTCCATTC AGGACTACAC GAGGCGCTGC 
GGCTCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540505.1
CDS	697..2988
Translation	MRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	XM_011542203.2
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542203.2

ORF Insert Sequence:

ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGATTAGC TATGCGGCCA GCAGCGAGAC GCTCAGCGTG 
AAGCGGCAGT ATCCCTCTTT CCTGCGCACC ATCCCCAATG ACAAGTACCA GGTGGAGACC 
ATGGTGCTGC TGCTGCAGAA GTTCGGGTGG ACCTGGATCT CTCTGGTTGG CAGCAGTGAC 
GACTATGGGC AGCTAGGGGT GCAGGCACTG GAGAACCAGG CCACTGGTCA GGGGATCTGC 
ATTGCTTTCA AGGACATCAT GCCCTTCTCT GCCCAGGTGG GCGATGAGAG GATGCAGTGC 
CTCATGCGCC ACCTGGCCCA GGCCGGGGCC ACCGTCGTGG TTGTTTTTTC CAGCCGGCAG 
TTGGCCAGGG TGTTTTTCGA GTCCGTGGTG CTGACCAACC TGACTGGCAA GGTGTGGGTC 
GCCTCAGAAG CCTGGGCCCT CTCCAGGCAC ATCACTGGGG TGCCCGGGAT CCAGCGCATT 
GGGATGGTGC TGGGCGTGGC CATCCAGAAG AGGGCTGTCC CTGGCCTGAA GGCGTTTGAA 
GAAGCCTATG CCCGGGCAGA CAAGAAGGCC CCTAGGCCTT GCCACAAGGG CTCCTGGTGC 
AGCAGCAATC AGCTCTGCAG AGAATGCCAA GCTTTCATGG CACACACGAT GCCCAAGCTC 
AAAGCCTTCT CCATGAGTTC TGCCTACAAC GCATACCGGG CTGTGTATGC GGTGGCCCAT 
GGCCTCCACC AGCTCCTGGG CTGTGCCTCT GGAGCTTGTT CCAGGGGCCG AGTCTACCCC 
TGGCAGCTTT TGGAGCAGAT CCACAAGGTG CATTTCCTTC TACACAAGGA CACTGTGGCG 
TTTAATGACA ACAGAGATCC CCTCAGTAGC TATAACATAA TTGCCTGGGA CTGGAATGGA 
CCCAAGTGGA CCTTCACGGT CCTCGGTTCC TCCACATGGT CTCCAGTTCA GCTAAACATA 
AATGAGACCA AAATCCAGTG GCACGGAAAG GACAACCAGG TGCCTAAGTC TGTGTGTTCC 
AGCGACTGTC TTGAAGGGCA CCAGCGAGTG GTTACGGGTT TCCATCACTG CTGCTTTGAG 
TGTGTGCCCT GTGGGGCTGG GACCTTCCTC AACAAGAGTG ACCTCTACAG ATGCCAGCCT 
TGTGGGAAAG AAGAGTGGGC ACCTGAGGGA AGCCAGACCT GCTTCCCGCG CACTGTGGTG 
TTTTTGGCTT TGCGTGAGCA CACCTCTTGG GTGCTGCTGG CAGCTAACAC GCTGCTGCTG 
CTGCTGCTGC TTGGGACTGC TGGCCTGTTT GCCTGGCACC TAGACACCCC TGTGGTGAGG 
TCAGCAGGGG GCCGCCTGTG CTTTCTTATG CTGGGCTCCC TGGCAGCAGG TAGTGGCAGC 
CTCTATGGCT TCTTTGGGGA ACCCACAAGG CCTGCGTGCT TGCTACGCCA GGCCCTCTTT 
GCCCTTGGTT TCACCATCTT CCTGTCCTGC CTGACAGTTC GCTCATTCCA ACTAATCATC 
ATCTTCAAGT TTTCCACCAA GGTACCTACA TTCTACCACG CCTGGGTCCA AAACCACGGT 
GCTGGCCTGT TTGTGATGAT CAGCTCAGCG GCCCAGCTGC TTATCTGTCT AACTTGGCTG 
GTGGTGTGGA CCCCACTGCC TGCTAGGGAA TACCAGCGCT TCCCCCATCT GGTGATGCTT 
GAGTGCACAG AGACCAACTC CCTGGGCTTC ATACTGGCCT TCCTCTACAA TGGCCTCCTC 
TCCATCAGTG CCTTTGCCTG CAGCTACCTG GGTAAGGACT TGCCAGAGAA CTACAACGAG 
GCCAAATGTG TCACCTTCAG CCTGCTCTTC AACTTCGTGT CCTGGATCGC CTTCTTCACC 
ACGGCCAGCG TCTACGACGG CAAGTACCTG CCTGCGGCCA ACATGATGGC TGGGCTGAGC 
AGCCTGAGCA GCGGCTTCGG TGGGTATTTT CTGCCTAAGT GCTACGTGAT CCTCTGCCGC 
CCAGACCTCA ACAGCACAGA GCACTTCCAG GCCTCCATTC AGGACTACAC GAGGCGCTGC 
GGCTCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( NM_177540.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_803884.1 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 3, mRNA.

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CloneID	OHu14934
Clone ID Related Accession (Same CDS sequence)	NM_177540.3 , NM_177540.2
Accession Version	NM_177540.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC136515.1, AK292014.1, AL591866.13 and AI742401.1. On May 31, 2019 this sequence version replaced NM_177540.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 2, resulting in an isoform (c) which is shorter but has the same N- and C-termini compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.16399.1, AK292014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGCT TTTGGAGCAG ATCCACAAGG TGCATTTCCT TCTACACAAG 
GACACTGTGG CGTTTAATGA CAACAGAGAT CCCCTCAGTA GCTATAACAT AATTGCCTGG 
GACTGGAATG GACCCAAGTG GACCTTCACG GTCCTCGGTT CCTCCACATG GTCTCCAGTT 
CAGCTAAACA TAAATGAGAC CAAAATCCAG TGGCACGGAA AGGACAACCA GGTGCCTAAG 
TCTGTGTGTT CCAGCGACTG TCTTGAAGGG CACCAGCGAG TGGTTACGGG TTTCCATCAC 
TGCTGCTTTG AGTGTGTGCC CTGTGGGGCT GGGACCTTCC TCAACAAGAG TGACCTCTAC 
AGATGCCAGC CTTGTGGGAA AGAAGAGTGG GCACCTGAGG GAAGCCAGAC CTGCTTCCCG 
CGCACTGTGG TGTTTTTGGC TTTGCGTGAG CACACCTCTT GGGTGCTGCT GGCAGCTAAC 
ACGCTGCTGC TGCTGCTGCT GCTTGGGACT GCTGGCCTGT TTGCCTGGCA CCTAGACACC 
CCTGTGGTGA GGTCAGCAGG GGGCCGCCTG TGCTTTCTTA TGCTGGGCTC CCTGGCAGCA 
GGTAGTGGCA GCCTCTATGG CTTCTTTGGG GAACCCACAA GGCCTGCGTG CTTGCTACGC 
CAGGCCCTCT TTGCCCTTGG TTTCACCATC TTCCTGTCCT GCCTGACAGT TCGCTCATTC 
CAACTAATCA TCATCTTCAA GTTTTCCACC AAGGTACCTA CATTCTACCA CGCCTGGGTC 
CAAAACCACG GTGCTGGCCT GTTTGTGATG ATCAGCTCAG CGGCCCAGCT GCTTATCTGT 
CTAACTTGGC TGGTGGTGTG GACCCCACTG CCTGCTAGGG AATACCAGCG CTTCCCCCAT 
CTGGTGATGC TTGAGTGCAC AGAGACCAAC TCCCTGGGCT TCATACTGGC CTTCCTCTAC 
AATGGCCTCC TCTCCATCAG TGCCTTTGCC TGCAGCTACC TGGGTAAGGA CTTGCCAGAG 
AACTACAACG AGGCCAAATG TGTCACCTTC AGCCTGCTCT TCAACTTCGT GTCCTGGATC 
GCCTTCTTCA CCACGGCCAG CGTCTACGAC GGCAAGTACC TGCCTGCGGC CAACATGATG 
GCTGGGCTGA GCAGCCTGAG CAGCGGCTTC GGTGGGTATT TTCTGCCTAA GTGCTACGTG 
ATCCTCTGCC GCCCAGACCT CAACAGCACA GAGCACTTCC AGGCCTCCAT TCAGGACTAC 
ACGAGGCGCT GCGGCTCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803884.1
CDS	68..1831
Translation	MLLCTARLVGLQLLISCCWAFACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	NM_177540.3
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177540.3

ORF Insert Sequence:

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGCT TTTGGAGCAG ATCCACAAGG TGCATTTCCT TCTACACAAG 
GACACTGTGG CGTTTAATGA CAACAGAGAT CCCCTCAGTA GCTATAACAT AATTGCCTGG 
GACTGGAATG GACCCAAGTG GACCTTCACG GTCCTCGGTT CCTCCACATG GTCTCCAGTT 
CAGCTAAACA TAAATGAGAC CAAAATCCAG TGGCACGGAA AGGACAACCA GGTGCCTAAG 
TCTGTGTGTT CCAGCGACTG TCTTGAAGGG CACCAGCGAG TGGTTACGGG TTTCCATCAC 
TGCTGCTTTG AGTGTGTGCC CTGTGGGGCT GGGACCTTCC TCAACAAGAG TGACCTCTAC 
AGATGCCAGC CTTGTGGGAA AGAAGAGTGG GCACCTGAGG GAAGCCAGAC CTGCTTCCCG 
CGCACTGTGG TGTTTTTGGC TTTGCGTGAG CACACCTCTT GGGTGCTGCT GGCAGCTAAC 
ACGCTGCTGC TGCTGCTGCT GCTTGGGACT GCTGGCCTGT TTGCCTGGCA CCTAGACACC 
CCTGTGGTGA GGTCAGCAGG GGGCCGCCTG TGCTTTCTTA TGCTGGGCTC CCTGGCAGCA 
GGTAGTGGCA GCCTCTATGG CTTCTTTGGG GAACCCACAA GGCCTGCGTG CTTGCTACGC 
CAGGCCCTCT TTGCCCTTGG TTTCACCATC TTCCTGTCCT GCCTGACAGT TCGCTCATTC 
CAACTAATCA TCATCTTCAA GTTTTCCACC AAGGTACCTA CATTCTACCA CGCCTGGGTC 
CAAAACCACG GTGCTGGCCT GTTTGTGATG ATCAGCTCAG CGGCCCAGCT GCTTATCTGT 
CTAACTTGGC TGGTGGTGTG GACCCCACTG CCTGCTAGGG AATACCAGCG CTTCCCCCAT 
CTGGTGATGC TTGAGTGCAC AGAGACCAAC TCCCTGGGCT TCATACTGGC CTTCCTCTAC 
AATGGCCTCC TCTCCATCAG TGCCTTTGCC TGCAGCTACC TGGGTAAGGA CTTGCCAGAG 
AACTACAACG AGGCCAAATG TGTCACCTTC AGCCTGCTCT TCAACTTCGT GTCCTGGATC 
GCCTTCTTCA CCACGGCCAG CGTCTACGAC GGCAAGTACC TGCCTGCGGC CAACATGATG 
GCTGGGCTGA GCAGCCTGAG CAGCGGCTTC GGTGGGTATT TTCTGCCTAA GTGCTACGTG 
ATCCTCTGCC GCCCAGACCT CAACAGCACA GAGCACTTCC AGGCCTCCAT TCAGGACTAC 
ACGAGGCGCT GCGGCTCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( NM_177540.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_803884.1 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 3, mRNA.

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CloneID	OHu14934
Clone ID Related Accession (Same CDS sequence)	NM_177540.3 , NM_177540.2
Accession Version	NM_177540.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC136515.1, AK292014.1, AL591866.13 and AI742401.1. On Jun 5, 2010 this sequence version replaced NM_177540.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 2, resulting in an isoform (c) which is shorter but has the same N- and C-termini compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.16399.1, AK292014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGCT TTTGGAGCAG ATCCACAAGG TGCATTTCCT TCTACACAAG 
GACACTGTGG CGTTTAATGA CAACAGAGAT CCCCTCAGTA GCTATAACAT AATTGCCTGG 
GACTGGAATG GACCCAAGTG GACCTTCACG GTCCTCGGTT CCTCCACATG GTCTCCAGTT 
CAGCTAAACA TAAATGAGAC CAAAATCCAG TGGCACGGAA AGGACAACCA GGTGCCTAAG 
TCTGTGTGTT CCAGCGACTG TCTTGAAGGG CACCAGCGAG TGGTTACGGG TTTCCATCAC 
TGCTGCTTTG AGTGTGTGCC CTGTGGGGCT GGGACCTTCC TCAACAAGAG TGACCTCTAC 
AGATGCCAGC CTTGTGGGAA AGAAGAGTGG GCACCTGAGG GAAGCCAGAC CTGCTTCCCG 
CGCACTGTGG TGTTTTTGGC TTTGCGTGAG CACACCTCTT GGGTGCTGCT GGCAGCTAAC 
ACGCTGCTGC TGCTGCTGCT GCTTGGGACT GCTGGCCTGT TTGCCTGGCA CCTAGACACC 
CCTGTGGTGA GGTCAGCAGG GGGCCGCCTG TGCTTTCTTA TGCTGGGCTC CCTGGCAGCA 
GGTAGTGGCA GCCTCTATGG CTTCTTTGGG GAACCCACAA GGCCTGCGTG CTTGCTACGC 
CAGGCCCTCT TTGCCCTTGG TTTCACCATC TTCCTGTCCT GCCTGACAGT TCGCTCATTC 
CAACTAATCA TCATCTTCAA GTTTTCCACC AAGGTACCTA CATTCTACCA CGCCTGGGTC 
CAAAACCACG GTGCTGGCCT GTTTGTGATG ATCAGCTCAG CGGCCCAGCT GCTTATCTGT 
CTAACTTGGC TGGTGGTGTG GACCCCACTG CCTGCTAGGG AATACCAGCG CTTCCCCCAT 
CTGGTGATGC TTGAGTGCAC AGAGACCAAC TCCCTGGGCT TCATACTGGC CTTCCTCTAC 
AATGGCCTCC TCTCCATCAG TGCCTTTGCC TGCAGCTACC TGGGTAAGGA CTTGCCAGAG 
AACTACAACG AGGCCAAATG TGTCACCTTC AGCCTGCTCT TCAACTTCGT GTCCTGGATC 
GCCTTCTTCA CCACGGCCAG CGTCTACGAC GGCAAGTACC TGCCTGCGGC CAACATGATG 
GCTGGGCTGA GCAGCCTGAG CAGCGGCTTC GGTGGGTATT TTCTGCCTAA GTGCTACGTG 
ATCCTCTGCC GCCCAGACCT CAACAGCACA GAGCACTTCC AGGCCTCCAT TCAGGACTAC 
ACGAGGCGCT GCGGCTCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803884.1
CDS	97..1860
Translation	MLLCTARLVGLQLLISCCWAFACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	NM_177540.2
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177540.2

ORF Insert Sequence:

ATGCTGCTCT GCACGGCTCG CCTGGTCGGC CTGCAGCTTC TCATTTCCTG CTGCTGGGCC 
TTTGCCTGCC ATAGCACGGA GTCTTCTCCT GACTTCACCC TCCCCGGAGA TTACCTCCTG 
GCAGGCCTGT TCCCTCTCCA TTCTGGCTGT CTGCAGGTGA GGCACAGACC CGAGGTGACC 
CTGTGTGACA GGTCTTGTAG CTTCAATGAG CATGGCTACC ACCTCTTCCA GGCTATGCGG 
CTTGGGGTTG AGGAGATAAA CAACTCCACG GCCCTGCTGC CCAACATCAC CCTGGGGTAC 
CAGCTGTATG ATGTGTGTTC TGACTCTGCC AATGTGTATG CCACGCTGAG AGTGCTCTCC 
CTGCCAGGGC AACACCACAT AGAGCTCCAA GGAGACCTTC TCCACTATTC CCCTACGGTG 
CTGGCAGTGA TTGGGCCTGA CAGCACCAAC CGTGCTGCCA CCACAGCCGC CCTGCTGAGC 
CCTTTCCTGG TGCCCATGCT TTTGGAGCAG ATCCACAAGG TGCATTTCCT TCTACACAAG 
GACACTGTGG CGTTTAATGA CAACAGAGAT CCCCTCAGTA GCTATAACAT AATTGCCTGG 
GACTGGAATG GACCCAAGTG GACCTTCACG GTCCTCGGTT CCTCCACATG GTCTCCAGTT 
CAGCTAAACA TAAATGAGAC CAAAATCCAG TGGCACGGAA AGGACAACCA GGTGCCTAAG 
TCTGTGTGTT CCAGCGACTG TCTTGAAGGG CACCAGCGAG TGGTTACGGG TTTCCATCAC 
TGCTGCTTTG AGTGTGTGCC CTGTGGGGCT GGGACCTTCC TCAACAAGAG TGACCTCTAC 
AGATGCCAGC CTTGTGGGAA AGAAGAGTGG GCACCTGAGG GAAGCCAGAC CTGCTTCCCG 
CGCACTGTGG TGTTTTTGGC TTTGCGTGAG CACACCTCTT GGGTGCTGCT GGCAGCTAAC 
ACGCTGCTGC TGCTGCTGCT GCTTGGGACT GCTGGCCTGT TTGCCTGGCA CCTAGACACC 
CCTGTGGTGA GGTCAGCAGG GGGCCGCCTG TGCTTTCTTA TGCTGGGCTC CCTGGCAGCA 
GGTAGTGGCA GCCTCTATGG CTTCTTTGGG GAACCCACAA GGCCTGCGTG CTTGCTACGC 
CAGGCCCTCT TTGCCCTTGG TTTCACCATC TTCCTGTCCT GCCTGACAGT TCGCTCATTC 
CAACTAATCA TCATCTTCAA GTTTTCCACC AAGGTACCTA CATTCTACCA CGCCTGGGTC 
CAAAACCACG GTGCTGGCCT GTTTGTGATG ATCAGCTCAG CGGCCCAGCT GCTTATCTGT 
CTAACTTGGC TGGTGGTGTG GACCCCACTG CCTGCTAGGG AATACCAGCG CTTCCCCCAT 
CTGGTGATGC TTGAGTGCAC AGAGACCAAC TCCCTGGGCT TCATACTGGC CTTCCTCTAC 
AATGGCCTCC TCTCCATCAG TGCCTTTGCC TGCAGCTACC TGGGTAAGGA CTTGCCAGAG 
AACTACAACG AGGCCAAATG TGTCACCTTC AGCCTGCTCT TCAACTTCGT GTCCTGGATC 
GCCTTCTTCA CCACGGCCAG CGTCTACGAC GGCAAGTACC TGCCTGCGGC CAACATGATG 
GCTGGGCTGA GCAGCCTGAG CAGCGGCTTC GGTGGGTATT TTCTGCCTAA GTGCTACGTG 
ATCCTCTGCC GCCCAGACCT CAACAGCACA GAGCACTTCC AGGCCTCCAT TCAGGACTAC 
ACGAGGCGCT GCGGCTCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( XM_011542206.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540508.1 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X4, mRNA.

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CloneID	OHu72852
Clone ID Related Accession (Same CDS sequence)	XM_011542206.2
Accession Version	XM_011542206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAATAA GTTTCTCCAT CAGGGCCAGC TTGTTCCTCC TACTGAGCTT GAGAGCCCTT 
GTTGAAGTTG TGGTTTGGGG GACTGGACCG ATGACCTCAA AGGTTCCCTT TGCTCCCAAG 
CCTCAGAGTC TAGGAGGCCA GAGGGTCTCA GCAGGCCTTT GTCCTTCTCA GCTGTCTCTT 
ACTGGCTTTC TCCACAGGTC TTGTAGCTTC AATGAGCATG GCTACCACCT CTTCCAGGCT 
ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGATTAGC TATGCGGCCA GCAGCGAGAC GCTCAGCGTG 
AAGCGGCAGT ATCCCTCTTT CCTGCGCACC ATCCCCAATG ACAAGTACCA GGTGGAGACC 
ATGGTGCTGC TGCTGCAGAA GTTCGGGTGG ACCTGGATCT CTCTGGTTGG CAGCAGTGAC 
GACTATGGGC AGCTAGGGGT GCAGGCACTG GAGAACCAGG CCACTGGTCA GGGGATCTGC 
ATTGCTTTCA AGGACATCAT GCCCTTCTCT GCCCAGGTGG GCGATGAGAG GATGCAGTGC 
CTCATGCGCC ACCTGGCCCA GGCCGGGGCC ACCGTCGTGG TTGTTTTTTC CAGCCGGCAG 
TTGGCCAGGG TGTTTTTCGA GTCCGTGGTG CTGACCAACC TGACTGGCAA GGTGTGGGTC 
GCCTCAGAAG CCTGGGCCCT CTCCAGGCAC ATCACTGGGG TGCCCGGGAT CCAGCGCATT 
GGGATGGTGC TGGGCGTGGC CATCCAGAAG AGGGCTGTCC CTGGCCTGAA GGCGTTTGAA 
GAAGCCTATG CCCGGGCAGA CAAGAAGGCC CCTAGGCCTT GCCACAAGGG CTCCTGGTGC 
AGCAGCAATC AGCTCTGCAG AGAATGCCAA GCTTTCATGG CACACACGAT GCCCAAGCTC 
AAAGCCTTCT CCATGAGTTC TGCCTACAAC GCATACCGGG CTGTGTATGC GGTGGCCCAT 
GGCCTCCACC AGCTCCTGGG CTGTGCCTCT GGAGCTTGTT CCAGGGGCCG AGTCTACCCC 
TGGCAGCTTT TGGAGCAGAT CCACAAGGTG CATTTCCTTC TACACAAGGA CACTGTGGCG 
TTTAATGACA ACAGAGATCC CCTCAGTAGC TATAACATAA TTGCCTGGGA CTGGAATGGA 
CCCAAGTGGA CCTTCACGGT CCTCGGTTCC TCCACATGGT CTCCAGTTCA GCTAAACATA 
AATGAGACCA AAATCCAGTG GCACGGAAAG GACAACCAGG TGCCTAAGTC TGTGTGTTCC 
AGCGACTGTC TTGAAGGGCA CCAGCGAGTG GTTACGGGTT TCCATCACTG CTGCTTTGAG 
TGTGTGCCCT GTGGGGCTGG GACCTTCCTC AACAAGAGTG CTACCTGGGT AAGGACTTGC 
CAGAGAACTA CAACGAGGCC AAATGTGTCA CCTTCAGCCT GCTCTTCAAC TTCGTGTCCT 
GGATCGCCTT CTTCACCACG GCCAGCGTCT ACGACGGCAA GTACCTGCCT GCGGCCAACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540508.1
CDS	643..2385
Translation	MLISFSIRASLFLLLSLRALVEVVVWGTGPMTSKVPFAPKPQSLGGQRVSAGLCPSQLSLTGFLHRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSATWVRTCQRTTTRPNVSPSACSSTSCPGSPSSPRPASTTASTCLRPT

Target ORF information:

RefSeq Version	XM_011542206.2
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542206.2

ORF Insert Sequence:

ATGCTAATAA GTTTCTCCAT CAGGGCCAGC TTGTTCCTCC TACTGAGCTT GAGAGCCCTT 
GTTGAAGTTG TGGTTTGGGG GACTGGACCG ATGACCTCAA AGGTTCCCTT TGCTCCCAAG 
CCTCAGAGTC TAGGAGGCCA GAGGGTCTCA GCAGGCCTTT GTCCTTCTCA GCTGTCTCTT 
ACTGGCTTTC TCCACAGGTC TTGTAGCTTC AATGAGCATG GCTACCACCT CTTCCAGGCT 
ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGATTAGC TATGCGGCCA GCAGCGAGAC GCTCAGCGTG 
AAGCGGCAGT ATCCCTCTTT CCTGCGCACC ATCCCCAATG ACAAGTACCA GGTGGAGACC 
ATGGTGCTGC TGCTGCAGAA GTTCGGGTGG ACCTGGATCT CTCTGGTTGG CAGCAGTGAC 
GACTATGGGC AGCTAGGGGT GCAGGCACTG GAGAACCAGG CCACTGGTCA GGGGATCTGC 
ATTGCTTTCA AGGACATCAT GCCCTTCTCT GCCCAGGTGG GCGATGAGAG GATGCAGTGC 
CTCATGCGCC ACCTGGCCCA GGCCGGGGCC ACCGTCGTGG TTGTTTTTTC CAGCCGGCAG 
TTGGCCAGGG TGTTTTTCGA GTCCGTGGTG CTGACCAACC TGACTGGCAA GGTGTGGGTC 
GCCTCAGAAG CCTGGGCCCT CTCCAGGCAC ATCACTGGGG TGCCCGGGAT CCAGCGCATT 
GGGATGGTGC TGGGCGTGGC CATCCAGAAG AGGGCTGTCC CTGGCCTGAA GGCGTTTGAA 
GAAGCCTATG CCCGGGCAGA CAAGAAGGCC CCTAGGCCTT GCCACAAGGG CTCCTGGTGC 
AGCAGCAATC AGCTCTGCAG AGAATGCCAA GCTTTCATGG CACACACGAT GCCCAAGCTC 
AAAGCCTTCT CCATGAGTTC TGCCTACAAC GCATACCGGG CTGTGTATGC GGTGGCCCAT 
GGCCTCCACC AGCTCCTGGG CTGTGCCTCT GGAGCTTGTT CCAGGGGCCG AGTCTACCCC 
TGGCAGCTTT TGGAGCAGAT CCACAAGGTG CATTTCCTTC TACACAAGGA CACTGTGGCG 
TTTAATGACA ACAGAGATCC CCTCAGTAGC TATAACATAA TTGCCTGGGA CTGGAATGGA 
CCCAAGTGGA CCTTCACGGT CCTCGGTTCC TCCACATGGT CTCCAGTTCA GCTAAACATA 
AATGAGACCA AAATCCAGTG GCACGGAAAG GACAACCAGG TGCCTAAGTC TGTGTGTTCC 
AGCGACTGTC TTGAAGGGCA CCAGCGAGTG GTTACGGGTT TCCATCACTG CTGCTTTGAG 
TGTGTGCCCT GTGGGGCTGG GACCTTCCTC AACAAGAGTG CTACCTGGGT AAGGACTTGC 
CAGAGAACTA CAACGAGGCC AAATGTGTCA CCTTCAGCCT GCTCTTCAAC TTCGTGTCCT 
GGATCGCCTT CTTCACCACG GCCAGCGTCT ACGACGGCAA GTACCTGCCT GCGGCCAACA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( XM_017002402.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857891.1 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu90569
Clone ID Related Accession (Same CDS sequence)	XM_017002402.1
Accession Version	XM_017002402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAATAA GTTTCTCCAT CAGGGCCAGC TTGTTCCTCC TACTGAGCTT GAGAGCCCTT 
GTTGAAGTTG TGGTTTGGGG GACTGGACCG ATGACCTCAA AGGTTCCCTT TGCTCCCAAG 
CCTCAGAGTC TAGGAGGCCA GAGGGTCTCA GCAGGCCTTT GTCCTTCTCA GCTGTCTCTT 
ACTGGCTTTC TCCACAGGTC TTGTAGCTTC AATGAGCATG GCTACCACCT CTTCCAGGCT 
ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGATTAGC TATGCGGCCA GCAGCGAGAC GCTCAGCGTG 
AAGCGGCAGT ATCCCTCTTT CCTGCGCACC ATCCCCAATG ACAAGTACCA GGTGGAGACC 
ATGGTGCTGC TGCTGCAGAA GTTCGGGTGG ACCTGGATCT CTCTGGTTGG CAGCAGTGAC 
GACTATGGGC AGCTAGGGGT GCAGGCACTG GAGAACCAGG CCACTGGTCA GGGGATCTGC 
ATTGCTTTCA AGGACATCAT GCCCTTCTCT GCCCAGGTGG GCGATGAGAG GATGCAGTGC 
CTCATGCGCC ACCTGGCCCA GGCCGGGGCC ACCGTCGTGG TTGTTTTTTC CAGCCGGCAG 
TTGGCCAGGG TGTTTTTCGA GTCCGTGGTG CTGACCAACC TGACTGGCAA GGTGTGGGTC 
GCCTCAGAAG CCTGGGCCCT CTCCAGGCAC ATCACTGGGG TGCCCGGGAT CCAGCGCATT 
GGGATGGTGC TGGGCGTGGC CATCCAGAAG AGGGCTGTCC CTGGCCTGAA GGCGTTTGAA 
GAAGCCTATG CCCGGGCAGA CAAGAAGGCC CCTAGGCCTT GCCACAAGGG CTCCTGGTGC 
AGCAGCAATC AGCTCTGCAG AGAATGCCAA GCTTTCATGG CACACACGAT GCCCAAGCTC 
AAAGCCTTCT CCATGAGTTC TGCCTACAAC GCATACCGGG CTGTGTATGC GGTGGCCCAT 
GGCCTCCACC AGCTCCTGGG CTGTGCCTCT GGAGCTTGTT CCAGGGGCCG AGTCTACCCC 
TGGCAGCTTT TGGAGCAGAT CCACAAGGTG CATTTCCTTC TACACAAGGA CACTGTGGCG 
TTTAATGACA ACAGAGATCC CCTCAGTAGC TATAACATAA TTGCCTGGGA CTGGAATGGA 
CCCAAGTGGA CCTTCACGGT CCTCGGTTCC TCCACATGGT CTCCAGTTCA GCTAAACATA 
AATGAGACCA AAATCCAGTG GCACGGAAAG GACAACCAGG TGCCTAAGTC TGTGTGTTCC 
AGCGACTGTC TTGAAGGGCA CCAGCGAGTG GTTACGGGTT TCCATCACTG CTGCTTTGAG 
TGTGTGCCCT GTGGGGCTGG GACCTTCCTC AACAAGAGTG ACCTCTACAG ATGCCAGCCT 
TGTGGGAAAG AAGAGTGGGC ACCTGAGGGA AGCCAGACCT GCTTCCCGCG CACTGTGGTG 
TTTTTGGCTT TGCGTGAGCA CACCTCTTGG GTGCTGCTGG CAGCTAACAC GCTGCTGCTG 
CTGCTGCTGC TTGGGACTGC TGGCCTGTTT GCCTGGCACC TAGACACCCC TGTGGTGAGG 
TCAGCAGGGG GCCGCCTGTG CTTTCTTATG CTGGGCTCCC TGGCAGCAGG TAGTGGCAGC 
CTCTATGGCT TCTTTGGGGA ACCCACAAGG CCTGCGTGCT TGCTACGCCA GGCCCTCTTT 
GCCCTTGGTT TCACCATCTT CCTGTCCTGC CTGACAGTTC GCTCATTCCA ACTAATCATC 
ATCTTCAAGT TTTCCACCAA GGTACCTACA TTCTACCACG CCTGGGTCCA AAACCACGGT 
GCTGGCCTGT TTGTGATGAT CAGCTCAGCG GCCCAGCTGC TTATCTGTCT AACTTGGCTG 
GTGGTGTGGA CCCCACTGCC TGCTAGGGAA TACCAGCGCT TCCCCCATCT GGTGATGCTT 
GAGTGCACAG AGACCAACTC CCTGGGCTTC ATACTGGCCT TCCTCTACAA TGGCCTCCTC 
TCCATCAGTG CCTTTGCCTG CAGCTACCTG GGTAAGGACT TGCCAGAGAA CTACAACGAG 
GCCAAATGTG TCACCTTCAG CCTGCTCTTC AACTTCGTGT CCTGGATCGC CTTCTTCACC 
ACGGCCAGCG TCTACGACGG CAAGTACCTG CCTGCGGCCA ACATGATGGC TGGGCTGAGC 
AGCCTGAGCA GCGGCTTCGG TGGGTATTTT CTGCCTAAGT GCTACGTGAT CCTCTGCCGC 
CCAGACCTCA ACAGCACAGA GCACTTCCAG GCCTCCATTC AGGACTACAC GAGGCGCTGC 
GGCTCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857891.1
CDS	643..3174
Translation	MLISFSIRASLFLLLSLRALVEVVVWGTGPMTSKVPFAPKPQSLGGQRVSAGLCPSQLSLTGFLHRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	XM_017002402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002402.1

ORF Insert Sequence:

ATGCTAATAA GTTTCTCCAT CAGGGCCAGC TTGTTCCTCC TACTGAGCTT GAGAGCCCTT 
GTTGAAGTTG TGGTTTGGGG GACTGGACCG ATGACCTCAA AGGTTCCCTT TGCTCCCAAG 
CCTCAGAGTC TAGGAGGCCA GAGGGTCTCA GCAGGCCTTT GTCCTTCTCA GCTGTCTCTT 
ACTGGCTTTC TCCACAGGTC TTGTAGCTTC AATGAGCATG GCTACCACCT CTTCCAGGCT 
ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGATTAGC TATGCGGCCA GCAGCGAGAC GCTCAGCGTG 
AAGCGGCAGT ATCCCTCTTT CCTGCGCACC ATCCCCAATG ACAAGTACCA GGTGGAGACC 
ATGGTGCTGC TGCTGCAGAA GTTCGGGTGG ACCTGGATCT CTCTGGTTGG CAGCAGTGAC 
GACTATGGGC AGCTAGGGGT GCAGGCACTG GAGAACCAGG CCACTGGTCA GGGGATCTGC 
ATTGCTTTCA AGGACATCAT GCCCTTCTCT GCCCAGGTGG GCGATGAGAG GATGCAGTGC 
CTCATGCGCC ACCTGGCCCA GGCCGGGGCC ACCGTCGTGG TTGTTTTTTC CAGCCGGCAG 
TTGGCCAGGG TGTTTTTCGA GTCCGTGGTG CTGACCAACC TGACTGGCAA GGTGTGGGTC 
GCCTCAGAAG CCTGGGCCCT CTCCAGGCAC ATCACTGGGG TGCCCGGGAT CCAGCGCATT 
GGGATGGTGC TGGGCGTGGC CATCCAGAAG AGGGCTGTCC CTGGCCTGAA GGCGTTTGAA 
GAAGCCTATG CCCGGGCAGA CAAGAAGGCC CCTAGGCCTT GCCACAAGGG CTCCTGGTGC 
AGCAGCAATC AGCTCTGCAG AGAATGCCAA GCTTTCATGG CACACACGAT GCCCAAGCTC 
AAAGCCTTCT CCATGAGTTC TGCCTACAAC GCATACCGGG CTGTGTATGC GGTGGCCCAT 
GGCCTCCACC AGCTCCTGGG CTGTGCCTCT GGAGCTTGTT CCAGGGGCCG AGTCTACCCC 
TGGCAGCTTT TGGAGCAGAT CCACAAGGTG CATTTCCTTC TACACAAGGA CACTGTGGCG 
TTTAATGACA ACAGAGATCC CCTCAGTAGC TATAACATAA TTGCCTGGGA CTGGAATGGA 
CCCAAGTGGA CCTTCACGGT CCTCGGTTCC TCCACATGGT CTCCAGTTCA GCTAAACATA 
AATGAGACCA AAATCCAGTG GCACGGAAAG GACAACCAGG TGCCTAAGTC TGTGTGTTCC 
AGCGACTGTC TTGAAGGGCA CCAGCGAGTG GTTACGGGTT TCCATCACTG CTGCTTTGAG 
TGTGTGCCCT GTGGGGCTGG GACCTTCCTC AACAAGAGTG ACCTCTACAG ATGCCAGCCT 
TGTGGGAAAG AAGAGTGGGC ACCTGAGGGA AGCCAGACCT GCTTCCCGCG CACTGTGGTG 
TTTTTGGCTT TGCGTGAGCA CACCTCTTGG GTGCTGCTGG CAGCTAACAC GCTGCTGCTG 
CTGCTGCTGC TTGGGACTGC TGGCCTGTTT GCCTGGCACC TAGACACCCC TGTGGTGAGG 
TCAGCAGGGG GCCGCCTGTG CTTTCTTATG CTGGGCTCCC TGGCAGCAGG TAGTGGCAGC 
CTCTATGGCT TCTTTGGGGA ACCCACAAGG CCTGCGTGCT TGCTACGCCA GGCCCTCTTT 
GCCCTTGGTT TCACCATCTT CCTGTCCTGC CTGACAGTTC GCTCATTCCA ACTAATCATC 
ATCTTCAAGT TTTCCACCAA GGTACCTACA TTCTACCACG CCTGGGTCCA AAACCACGGT 
GCTGGCCTGT TTGTGATGAT CAGCTCAGCG GCCCAGCTGC TTATCTGTCT AACTTGGCTG 
GTGGTGTGGA CCCCACTGCC TGCTAGGGAA TACCAGCGCT TCCCCCATCT GGTGATGCTT 
GAGTGCACAG AGACCAACTC CCTGGGCTTC ATACTGGCCT TCCTCTACAA TGGCCTCCTC 
TCCATCAGTG CCTTTGCCTG CAGCTACCTG GGTAAGGACT TGCCAGAGAA CTACAACGAG 
GCCAAATGTG TCACCTTCAG CCTGCTCTTC AACTTCGTGT CCTGGATCGC CTTCTTCACC 
ACGGCCAGCG TCTACGACGG CAAGTACCTG CCTGCGGCCA ACATGATGGC TGGGCTGAGC 
AGCCTGAGCA GCGGCTTCGG TGGGTATTTT CTGCCTAAGT GCTACGTGAT CCTCTGCCGC 
CCAGACCTCA ACAGCACAGA GCACTTCCAG GCCTCCATTC AGGACTACAC GAGGCGCTGC 
GGCTCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS1R1 ( XM_017002403.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857892.1 Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X3, mRNA.

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CloneID	OHu90570
Clone ID Related Accession (Same CDS sequence)	XM_017002403.1
Accession Version	XM_017002403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	taste receptor type 1 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAATAA GTTTCTCCAT CAGGGCCAGC TTGTTCCTCC TACTGAGCTT GAGAGCCCTT 
GTTGAAGTTG TGGTTTGGGG GACTGGACCG ATGACCTCAA AGGTTCCCTT TGCTCCCAAG 
CCTCAGAGTC TAGGAGGCCA GAGGGTCTCA GCAGGCCTTT GTCCTTCTCA GCTGTCTCTT 
ACTGGCTTTC TCCACAGGTC TTGTAGCTTC AATGAGCATG GCTACCACCT CTTCCAGGCT 
ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGCTTTTG GAGCAGATCC ACAAGGTGCA TTTCCTTCTA 
CACAAGGACA CTGTGGCGTT TAATGACAAC AGAGATCCCC TCAGTAGCTA TAACATAATT 
GCCTGGGACT GGAATGGACC CAAGTGGACC TTCACGGTCC TCGGTTCCTC CACATGGTCT 
CCAGTTCAGC TAAACATAAA TGAGACCAAA ATCCAGTGGC ACGGAAAGGA CAACCAGGTG 
CCTAAGTCTG TGTGTTCCAG CGACTGTCTT GAAGGGCACC AGCGAGTGGT TACGGGTTTC 
CATCACTGCT GCTTTGAGTG TGTGCCCTGT GGGGCTGGGA CCTTCCTCAA CAAGAGTGAC 
CTCTACAGAT GCCAGCCTTG TGGGAAAGAA GAGTGGGCAC CTGAGGGAAG CCAGACCTGC 
TTCCCGCGCA CTGTGGTGTT TTTGGCTTTG CGTGAGCACA CCTCTTGGGT GCTGCTGGCA 
GCTAACACGC TGCTGCTGCT GCTGCTGCTT GGGACTGCTG GCCTGTTTGC CTGGCACCTA 
GACACCCCTG TGGTGAGGTC AGCAGGGGGC CGCCTGTGCT TTCTTATGCT GGGCTCCCTG 
GCAGCAGGTA GTGGCAGCCT CTATGGCTTC TTTGGGGAAC CCACAAGGCC TGCGTGCTTG 
CTACGCCAGG CCCTCTTTGC CCTTGGTTTC ACCATCTTCC TGTCCTGCCT GACAGTTCGC 
TCATTCCAAC TAATCATCAT CTTCAAGTTT TCCACCAAGG TACCTACATT CTACCACGCC 
TGGGTCCAAA ACCACGGTGC TGGCCTGTTT GTGATGATCA GCTCAGCGGC CCAGCTGCTT 
ATCTGTCTAA CTTGGCTGGT GGTGTGGACC CCACTGCCTG CTAGGGAATA CCAGCGCTTC 
CCCCATCTGG TGATGCTTGA GTGCACAGAG ACCAACTCCC TGGGCTTCAT ACTGGCCTTC 
CTCTACAATG GCCTCCTCTC CATCAGTGCC TTTGCCTGCA GCTACCTGGG TAAGGACTTG 
CCAGAGAACT ACAACGAGGC CAAATGTGTC ACCTTCAGCC TGCTCTTCAA CTTCGTGTCC 
TGGATCGCCT TCTTCACCAC GGCCAGCGTC TACGACGGCA AGTACCTGCC TGCGGCCAAC 
ATGATGGCTG GGCTGAGCAG CCTGAGCAGC GGCTTCGGTG GGTATTTTCT GCCTAAGTGC 
TACGTGATCC TCTGCCGCCC AGACCTCAAC AGCACAGAGC ACTTCCAGGC CTCCATTCAG 
GACTACACGA GGCGCTGCGG CTCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857892.1
CDS	643..2412
Translation	MLISFSIRASLFLLLSLRALVEVVVWGTGPMTSKVPFAPKPQSLGGQRVSAGLCPSQLSLTGFLHRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Target ORF information:

RefSeq Version	XM_017002403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 1 receptor member 1 (TAS1R1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002403.1

ORF Insert Sequence:

ATGCTAATAA GTTTCTCCAT CAGGGCCAGC TTGTTCCTCC TACTGAGCTT GAGAGCCCTT 
GTTGAAGTTG TGGTTTGGGG GACTGGACCG ATGACCTCAA AGGTTCCCTT TGCTCCCAAG 
CCTCAGAGTC TAGGAGGCCA GAGGGTCTCA GCAGGCCTTT GTCCTTCTCA GCTGTCTCTT 
ACTGGCTTTC TCCACAGGTC TTGTAGCTTC AATGAGCATG GCTACCACCT CTTCCAGGCT 
ATGCGGCTTG GGGTTGAGGA GATAAACAAC TCCACGGCCC TGCTGCCCAA CATCACCCTG 
GGGTACCAGC TGTATGATGT GTGTTCTGAC TCTGCCAATG TGTATGCCAC GCTGAGAGTG 
CTCTCCCTGC CAGGGCAACA CCACATAGAG CTCCAAGGAG ACCTTCTCCA CTATTCCCCT 
ACGGTGCTGG CAGTGATTGG GCCTGACAGC ACCAACCGTG CTGCCACCAC AGCCGCCCTG 
CTGAGCCCTT TCCTGGTGCC CATGCTTTTG GAGCAGATCC ACAAGGTGCA TTTCCTTCTA 
CACAAGGACA CTGTGGCGTT TAATGACAAC AGAGATCCCC TCAGTAGCTA TAACATAATT 
GCCTGGGACT GGAATGGACC CAAGTGGACC TTCACGGTCC TCGGTTCCTC CACATGGTCT 
CCAGTTCAGC TAAACATAAA TGAGACCAAA ATCCAGTGGC ACGGAAAGGA CAACCAGGTG 
CCTAAGTCTG TGTGTTCCAG CGACTGTCTT GAAGGGCACC AGCGAGTGGT TACGGGTTTC 
CATCACTGCT GCTTTGAGTG TGTGCCCTGT GGGGCTGGGA CCTTCCTCAA CAAGAGTGAC 
CTCTACAGAT GCCAGCCTTG TGGGAAAGAA GAGTGGGCAC CTGAGGGAAG CCAGACCTGC 
TTCCCGCGCA CTGTGGTGTT TTTGGCTTTG CGTGAGCACA CCTCTTGGGT GCTGCTGGCA 
GCTAACACGC TGCTGCTGCT GCTGCTGCTT GGGACTGCTG GCCTGTTTGC CTGGCACCTA 
GACACCCCTG TGGTGAGGTC AGCAGGGGGC CGCCTGTGCT TTCTTATGCT GGGCTCCCTG 
GCAGCAGGTA GTGGCAGCCT CTATGGCTTC TTTGGGGAAC CCACAAGGCC TGCGTGCTTG 
CTACGCCAGG CCCTCTTTGC CCTTGGTTTC ACCATCTTCC TGTCCTGCCT GACAGTTCGC 
TCATTCCAAC TAATCATCAT CTTCAAGTTT TCCACCAAGG TACCTACATT CTACCACGCC 
TGGGTCCAAA ACCACGGTGC TGGCCTGTTT GTGATGATCA GCTCAGCGGC CCAGCTGCTT 
ATCTGTCTAA CTTGGCTGGT GGTGTGGACC CCACTGCCTG CTAGGGAATA CCAGCGCTTC 
CCCCATCTGG TGATGCTTGA GTGCACAGAG ACCAACTCCC TGGGCTTCAT ACTGGCCTTC 
CTCTACAATG GCCTCCTCTC CATCAGTGCC TTTGCCTGCA GCTACCTGGG TAAGGACTTG 
CCAGAGAACT ACAACGAGGC CAAATGTGTC ACCTTCAGCC TGCTCTTCAA CTTCGTGTCC 
TGGATCGCCT TCTTCACCAC GGCCAGCGTC TACGACGGCA AGTACCTGCC TGCGGCCAAC 
ATGATGGCTG GGCTGAGCAG CCTGAGCAGC GGCTTCGGTG GGTATTTTCT GCCTAAGTGC 
TACGTGATCC TCTGCCGCCC AGACCTCAAC AGCACAGAGC ACTTCCAGGC CTCCATTCAG 
GACTACACGA GGCGCTGCGG CTCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variations in TAS1R taste receptor gene family modify food intake and gastric cancer risk in a Korean population.
Molecular nutrition & food research60(11)2433-2445(2016 Nov)
Choi JH,Lee J,Choi IJ,Kim YW,Ryu KW,Kim J

Two distinct determinants of ligand specificity in T1R1/T1R3 (the umami taste receptor).
The Journal of biological chemistry288(52)36863-77(2013 Dec)
Toda Y,Nakagita T,Hayakawa T,Okada S,Narukawa M,Imai H,Ishimaru Y,Misaka T

Do polymorphisms in the TAS1R1 gene contribute to broader differences in human taste intensity?
Chemical senses38(8)719-28(2013 Oct)
Rawal S,Hayes JE,Wallace MR,Bartoshuk LM,Duffy VB

Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca?? and cAMP concentrations in spermatozoa.
PloS one7(2)e32354(2012)
Meyer D,Voigt A,Widmayer P,Borth H,Huebner S,Breit A,Marschall S,de Angelis MH,Boehm U,Meyerhof W,Gudermann T,Boekhoff I

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TAS1R1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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