Capza3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capza3
Entrez Gene ID	29324
Full Name	capping actin protein of muscle Z-line alpha subunit 3
Synonyms	Cappa3
General protein information	Preferred Names capping actin protein of muscle Z-line alpha subunit 3 Names F-actin-capping protein subunit alpha-3 capZ alpha-3 capping protein (actin filament) muscle Z-line, alpha 3 capping protein alpha 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q44
Summary	putative actin capping protein that may be involved in sperm head formation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017164.2	NP_058860.2	F-actin-capping protein subunit alpha-3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CAPZA3	NP_201585.1
	CAPZA3	XP_001096292.1
Mus musculus (house mouse)	Capza3	NP_031631.3
Gallus gallus (chicken)	CAPZA3	NP_001265047.1
Canis lupus familiaris (dog)	CAPZA3	XP_534875.1
Bos taurus (cattle)	CAPZA3	NP_001035651.1
Pan troglodytes (chimpanzee)	CAPZA3	NP_001233504.1
Rattus norvegicus (Norway rat)	Capza3	NP_058860.2

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

A missense mutation in the Capza3 gene and disruption of F-actin organization in spermatids of repro32 infertile male mice.
Geyer CB, Inselman AL, Sunman JA, Bornstein S, Handel MA, Eddy EM
Developmental biology330(1)142-52(2009 Jun)

Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice.
Robledo RF, Ciciotte SL, Gwynn B, Sahr KE, Gilligan DM, Mohandas N, Peters LL
Blood112(10)4298-307(2008 Nov)

Expression of a testis-specific putative actin-capping protein associated with the developing acrosome during rat spermiogenesis.
Hurst S, Howes EA, Coadwell J, Jones R
Molecular reproduction and development49(1)81-91(1998 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Capza3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capza3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42591	NM_017164.2 Latest version!	Rattus norvegicus capping actin protein of muscle Z-line subunit alpha 3 (Capza3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capza3 ( NM_017164.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058860.2 Rattus norvegicus capping actin protein of muscle Z-line subunit alpha 3 (Capza3), mRNA.

Price & Availability↑

CloneID	ORa42591
Clone ID Related Accession (Same CDS sequence)	NM_017164.2
Accession Version	NM_017164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	F-actin-capping protein subunit alpha-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC078870.1. On Nov 23, 2005 this sequence version replaced NM_017164.1. ##Evidence-Data-START## Transcript is intronless :: BC078870.1 [ECO:0000345] ##Evidence-Data-END##

ATGTCACTCA GCGTCTTAAG TAGGCAAGAC AAAGAAAAGG TCATCCACAG ACTCTTAATC 
CAGGCTCCTC CTGGGGAATT CGTGAATGCC TTTGATGATC TCTGTCTGCT TATCCGAGAT 
GAGAAGCTCA TGCACCACCA GGGTGAGTGC GCAGGTCATC AGCACTGCCA AAAATACTGT 
GTCCCACTCT GCATCGATGG CAATCCAGTC CTCTTGTCTC ACCACAATGT GATGGGTGAC 
TTCCGGTTCT TTGACTACCA GAGCAAACTT TCTTTCAGGT TTGACCTGCT CCAGAATCAG 
CTGAGAGACA TCCAAAGCCA CGGAATCATT CGGAATGAGA CCGAGTACCT GAGATCTGTT 
GTTATGTGTG CCTTAAAACT CTACGTGAAC GATCACTACC CAAACGGCAA CTGCAACGTG 
CTGAGGAAAA CAGTCAAAAA CAAGGAGTTC TTAATAGCTT GCATTGAAGA CCACAGCTAT 
GACAATGGAG AGTGTTGGAA TGGTCTTTGG AAGTCTAAGT GGATCTTCCA GGTAAACCCG 
TTTCTAACCC AAGTGACAGG AAGGATTTTT GTGCAAGCTC ACTACTTCAG ATGTGTCAAC 
CTTCATGTTG AAGTCTCCAA GGACCTCAAG GAGAGCTTGG AGGTGGTCAA CCAAGCCCAG 
TTGGCTCTCA GTTTCGCAAG GCTTGTGGAA GAACAAGAAA ATAAGTTTCA AGCTGCTGTA 
ATAGAAGAAC TACAGGAGTT GTCTAATGAA GCCCTGAGGA AAATATTACG GAGGGATCTT 
CCAGTGACAC GCACTTTGAT TGACTGGCAA CGGATCCTCT CTGACTTGAA TCTGGTGATG 
TACCCCAAAT TAGGATACGT TATTTACTCC AGGAGTGTGC TGTGCAACTG GATAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058860.2
CDS	104..1003
Translation	MSLSVLSRQDKEKVIHRLLIQAPPGEFVNAFDDLCLLIRDEKLMHHQGECAGHQHCQKYCVPLCIDGNPVLLSHHNVMGDFRFFDYQSKLSFRFDLLQNQLRDIQSHGIIRNETEYLRSVVMCALKLYVNDHYPNGNCNVLRKTVKNKEFLIACIEDHSYDNGECWNGLWKSKWIFQVNPFLTQVTGRIFVQAHYFRCVNLHVEVSKDLKESLEVVNQAQLALSFARLVEEQENKFQAAVIEELQELSNEALRKILRRDLPVTRTLIDWQRILSDLNLVMYPKLGYVIYSRSVLCNWII

Target ORF information:

RefSeq Version	NM_017164.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus capping actin protein of muscle Z-line subunit alpha 3 (Capza3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017164.2

ORF Insert Sequence:

ATGTCACTCA GCGTCTTAAG TAGGCAAGAC AAAGAAAAGG TCATCCACAG ACTCTTAATC 
CAGGCTCCTC CTGGGGAATT CGTGAATGCC TTTGATGATC TCTGTCTGCT TATCCGAGAT 
GAGAAGCTCA TGCACCACCA GGGTGAGTGC GCAGGTCATC AGCACTGCCA AAAATACTGT 
GTCCCACTCT GCATCGATGG CAATCCAGTC CTCTTGTCTC ACCACAATGT GATGGGTGAC 
TTCCGGTTCT TTGACTACCA GAGCAAACTT TCTTTCAGGT TTGACCTGCT CCAGAATCAG 
CTGAGAGACA TCCAAAGCCA CGGAATCATT CGGAATGAGA CCGAGTACCT GAGATCTGTT 
GTTATGTGTG CCTTAAAACT CTACGTGAAC GATCACTACC CAAACGGCAA CTGCAACGTG 
CTGAGGAAAA CAGTCAAAAA CAAGGAGTTC TTAATAGCTT GCATTGAAGA CCACAGCTAT 
GACAATGGAG AGTGTTGGAA TGGTCTTTGG AAGTCTAAGT GGATCTTCCA GGTAAACCCG 
TTTCTAACCC AAGTGACAGG AAGGATTTTT GTGCAAGCTC ACTACTTCAG ATGTGTCAAC 
CTTCATGTTG AAGTCTCCAA GGACCTCAAG GAGAGCTTGG AGGTGGTCAA CCAAGCCCAG 
TTGGCTCTCA GTTTCGCAAG GCTTGTGGAA GAACAAGAAA ATAAGTTTCA AGCTGCTGTA 
ATAGAAGAAC TACAGGAGTT GTCTAATGAA GCCCTGAGGA AAATATTACG GAGGGATCTT 
CCAGTGACAC GCACTTTGAT TGACTGGCAA CGGATCCTCT CTGACTTGAA TCTGGTGATG 
TACCCCAAAT TAGGATACGT TATTTACTCC AGGAGTGTGC TGTGCAACTG GATAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

A missense mutation in the Capza3 gene and disruption of F-actin organization in spermatids of repro32 infertile male mice.
Developmental biology330(1)142-52(2009 Jun)
Geyer CB,Inselman AL,Sunman JA,Bornstein S,Handel MA,Eddy EM

Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice.
Blood112(10)4298-307(2008 Nov)
Robledo RF,Ciciotte SL,Gwynn B,Sahr KE,Gilligan DM,Mohandas N,Peters LL

Expression of a testis-specific putative actin-capping protein associated with the developing acrosome during rat spermiogenesis.
Molecular reproduction and development49(1)81-91(1998 Jan)
Hurst S,Howes EA,Coadwell J,Jones R