Eed cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eed
Entrez Gene ID	293104
Full Name	embryonic ectoderm development
General protein information	Preferred Names embryonic ectoderm development Names polycomb protein EED
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229664.2	XP_006229726.1	polycomb protein EED isoform X1
NM_001106278.2	NP_001099748.1	polycomb protein EED

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EED	NP_001026547.1
Homo sapiens (human)	EED	NP_003788.2
Canis lupus familiaris (dog)	EED	XP_005633449.1
Danio rerio (zebrafish)	eed	NP_001017766.1
	EED	XP_001102402.1
Mus musculus (house mouse)	Eed	NP_068676.1
Drosophila melanogaster (fruit fly)	escl	NP_723702.1
Arabidopsis thaliana (thale cress)	FIE	NP_188710.1
Oryza sativa (rice)	Os08g0137100	NP_001060953.1
Bos taurus (cattle)	EED	NP_001035584.1
Drosophila melanogaster (fruit fly)	esc	NP_477431.1
Xenopus tropicalis (tropical clawed frog)	eed	NP_001017325.1
Rattus norvegicus (Norway rat)	Eed	NP_001099748.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in genes encoding polycomb repressive complex 2 subunits cause Weaver syndrome.
Imagawa E, Higashimoto K, Sakai Y, Numakura C, Okamoto N, Matsunaga S, Ryo A, Sato Y, Sanefuji M, Ihara K, Takada Y, Nishimura G, Saitsu H, Mizuguchi T, Miyatake S, Nakashima M, Miyake N, Soejima H, Matsumoto N
Human mutation38(6)637-648(2017 Jun)

SET for life: biochemical activities and biological functions of SET domain-containing proteins.
Herz HM, Garruss A, Shilatifard A
Trends in biochemical sciences38(12)621-39(2013 Dec)

Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors.
Wajapeyee N, Malonia SK, Palakurthy RK, Green MR
Genes & development27(20)2221-6(2013 Oct)

Early activation of nSMase2/ceramide pathway in astrocytes is involved in ischemia-associated neuronal damage via inflammation in rat hippocampi.
Gu L, Huang B, Shen W, Gao L, Ding Z, Wu H, Guo J
Journal of neuroinflammation10109(2013 Sep)

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Simon JA, Kingston RE
Molecular cell49(5)808-24(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eed gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eed cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04536	NM_001106278.2 Latest version!	Rattus norvegicus embryonic ectoderm development (Eed), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ORa23071	XM_006229664.2 Latest version!	Rattus norvegicus embryonic ectoderm development (Eed), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eed ( NM_001106278.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099748.1 Rattus norvegicus embryonic ectoderm development (Eed), mRNA.

Price & Availability↑

CloneID	ORa04536
Clone ID Related Accession (Same CDS sequence)	NM_001106278.2
Accession Version	NM_001106278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	polycomb protein EED
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC168872.1. On Sep 4, 2008 this sequence version replaced NM_001106278.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC168872.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGAGA GGGAAGTGTC GACTGCGCCG GCGGGAACAG ACATGCCGGC GGCCAAGAAG 
CAGAAGTTGA GCAGCGACGA GAACAGCAAC CCGGACCTCT CGGGAGACGA GAATGACGAT 
GCTGTCAGTA TCGAGAGTGG CACAAACACA GAACGCCCGG ACACACCCAC AAATACACCA 
AATGCACCAG GAAGGAAAAG CTGGGGAAAG GGAAAATGGA AGTCGAAGAA ATGCAAATAT 
TCTTTCAAAT GTGTGAACAG CCTCAAGGAA GATCACAACC AGCCATTGTT TGGAGTTCAG 
TTTAACTGGC ACAGTAAAGA AGGAGATCCT CTGGTGTTTG CAACTGTGGG AAGCAACAGA 
GTAACCTTAT ATGAATGCCA TTCTCAAGGG GAAATACGGT TATTGCAGTC CTATGTAGAT 
GCTGATGCAG ATGAAAACTT TTACACTTGT GCATGGACCT ATGATAGCAA CACCAGCCAC 
CCTCTGCTAG CAGTTGCTGG ATCTAGAGGC ATAATAAGAA TAATTAATCC TATAACAATG 
CAGTGTATAA AGCACTATGT TGGCCATGGG AATGCTATCA ATGAGCTGAA ATTCCACCCA 
CGAGACCCAA ACCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGATT ATGGAATATC 
CAAACAGACA CTCTTGTGGC AATATTCGGA GGTGTGGAAG GGCACAGAGA TGAAGTGCTG 
AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCCT GTGGTATGGA TCACTCTCTT 
AAACTGTGGA GAATCAACTC AAAGAGAATG ATGAATGCGA TTAAGGAATC TTACGATTAT 
AACCCAAACA AAACTAACAG GCCATTTATT TCCCAGAAAA TCCACTTTCC TGATTTTTCT 
ACCAGAGACA TACATAGGAA TTATGTTGAT TGTGTACGAT GGTTAGGCGA TTTGATACTT 
TCCAAGTCTT GTGAAAATGC CATTGTATGC TGGAAACCTG GCAAAATGGA GGATGATATA 
GATAAAATTA AACCTAGTGA GTCTAATGTG ACTATTCTTG GGCGATTTGA TTACAGCCAG 
TGTGACATTT GGTACATGAG GTTTTCTATG GATTTCTGGC AAAAGATGCT TGCATTGGGC 
AATCAGGTTG GCAAACTGTA TGTTTGGGAT TTAGAAGTAG AAGATCCTCA TAAAGCCAAA 
TGCACAACAC TGACCCATCA TAAATGTGGC GCTGCTATTC GACAAACCAG TTTTAGCAGG 
GATAGCAGCA TCCTCATAGC TGTCTGCGAT GATGCCAGCA TTTGGCGTTG GGATCGACTT 
CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099748.1
CDS	424..1749
Translation	MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR

Target ORF information:

RefSeq Version	NM_001106278.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus embryonic ectoderm development (Eed), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106278.2

ORF Insert Sequence:

ATGTCTGAGA GGGAAGTGTC GACTGCGCCG GCGGGAACAG ACATGCCGGC GGCCAAGAAG 
CAGAAGTTGA GCAGCGACGA GAACAGCAAC CCGGACCTCT CGGGAGACGA GAATGACGAT 
GCTGTCAGTA TCGAGAGTGG CACAAACACA GAACGCCCGG ACACACCCAC AAATACACCA 
AATGCACCAG GAAGGAAAAG CTGGGGAAAG GGAAAATGGA AGTCGAAGAA ATGCAAATAT 
TCTTTCAAAT GTGTGAACAG CCTCAAGGAA GATCACAACC AGCCATTGTT TGGAGTTCAG 
TTTAACTGGC ACAGTAAAGA AGGAGATCCT CTGGTGTTTG CAACTGTGGG AAGCAACAGA 
GTAACCTTAT ATGAATGCCA TTCTCAAGGG GAAATACGGT TATTGCAGTC CTATGTAGAT 
GCTGATGCAG ATGAAAACTT TTACACTTGT GCATGGACCT ATGATAGCAA CACCAGCCAC 
CCTCTGCTAG CAGTTGCTGG ATCTAGAGGC ATAATAAGAA TAATTAATCC TATAACAATG 
CAGTGTATAA AGCACTATGT TGGCCATGGG AATGCTATCA ATGAGCTGAA ATTCCACCCA 
CGAGACCCAA ACCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGATT ATGGAATATC 
CAAACAGACA CTCTTGTGGC AATATTCGGA GGTGTGGAAG GGCACAGAGA TGAAGTGCTG 
AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCCT GTGGTATGGA TCACTCTCTT 
AAACTGTGGA GAATCAACTC AAAGAGAATG ATGAATGCGA TTAAGGAATC TTACGATTAT 
AACCCAAACA AAACTAACAG GCCATTTATT TCCCAGAAAA TCCACTTTCC TGATTTTTCT 
ACCAGAGACA TACATAGGAA TTATGTTGAT TGTGTACGAT GGTTAGGCGA TTTGATACTT 
TCCAAGTCTT GTGAAAATGC CATTGTATGC TGGAAACCTG GCAAAATGGA GGATGATATA 
GATAAAATTA AACCTAGTGA GTCTAATGTG ACTATTCTTG GGCGATTTGA TTACAGCCAG 
TGTGACATTT GGTACATGAG GTTTTCTATG GATTTCTGGC AAAAGATGCT TGCATTGGGC 
AATCAGGTTG GCAAACTGTA TGTTTGGGAT TTAGAAGTAG AAGATCCTCA TAAAGCCAAA 
TGCACAACAC TGACCCATCA TAAATGTGGC GCTGCTATTC GACAAACCAG TTTTAGCAGG 
GATAGCAGCA TCCTCATAGC TGTCTGCGAT GATGCCAGCA TTTGGCGTTG GGATCGACTT 
CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eed ( XM_006229664.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229726.1 Rattus norvegicus embryonic ectoderm development (Eed), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23071
Clone ID Related Accession (Same CDS sequence)	XM_006229664.2
Accession Version	XM_006229664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	polycomb protein EED isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006229664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGA GGGAAGTGTC GACTGCGCCG GCGGGAACAG ACATGCCGGC GGCCAAGAAG 
CAGAAGTTGA GCAGCGACGA GAACAGCAAC CCGGACCTCT CGGGAGACGA GAATGACGAT 
GCTGTCAGTA TCGAGAGTGG CACAAACACA GAACGCCCGG ACACACCCAC AAATACACCA 
AATGCACCAG GAAGGAAAAG CTGGGGAAAG GGAAAATGGA AGTCGAAGAA ATGCAAATAT 
TCTTTCAAAT GTGTGAACAG CCTCAAGGAA GATCACAACC AGCCATTGTT TGGAGTTCAG 
TTTAACTGGC ACAGTAAAGA AGGAGATCCT CTGGTGTTTG CAACTGTGGG AAGCAACAGA 
GTAACCTTAT ATGAATGCCA TTCTCAAGGG GAAATACGGT TATTGCAGTC CTATGTAGAT 
GCTGATGCAG ATGAAAACTT TTACACTTGT GCATGGACCT ATGATAGCAA CACCAGCCAC 
CCTCTGCTAG CAGTTGCTGG ATCTAGAGGC ATAATAAGAA TAATTAATCC TATAACAATG 
CAGTGTATAA AGCACTATGT TGGCCATGGG AATGCTATCA ATGAGCTGAA ATTCCACCCA 
CGAGACCCAA ACCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGATT ATGGAATATC 
CAAACAGACA CTCTTGTGGC AATATTCGGA GGTGTGGAAG GGCACAGAGA TGAAGTGCTG 
AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCCT GTGGTATGGA TCACTCTCTT 
AAACTGTGGA GAATCAACTC AAAGAGAATG ATGAATGCGA TTAAGGAATC TTACGATTAT 
AACCCAAACA AAACTAACAG GCCATTTATT TCCCAGAAAA TCCACTTTCC TGATTTTTCT 
ACCAGAGACA TACATAGGAA TTATGTTGAT TGTGTACGAT GGTTAGGCGA TTTGATACTT 
TCCAAGTCTT GTGAAAATGC CATTGTATGC TGGAAACCTG GCAAAATGGA GGATGATATA 
GATAAAATTA AACCTAATGC TTGCATTGGG CAATCAGGTT GGCAAACTGT ATGTTTGGGA 
TTTAGAAGTA GAAGATCCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229726.1
CDS	203..1306
Translation	MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPNACIGQSGWQTVCLGFRSRRSS

Target ORF information:

RefSeq Version	XM_006229664.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus embryonic ectoderm development (Eed), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229664.2

ORF Insert Sequence:

ATGTCTGAGA GGGAAGTGTC GACTGCGCCG GCGGGAACAG ACATGCCGGC GGCCAAGAAG 
CAGAAGTTGA GCAGCGACGA GAACAGCAAC CCGGACCTCT CGGGAGACGA GAATGACGAT 
GCTGTCAGTA TCGAGAGTGG CACAAACACA GAACGCCCGG ACACACCCAC AAATACACCA 
AATGCACCAG GAAGGAAAAG CTGGGGAAAG GGAAAATGGA AGTCGAAGAA ATGCAAATAT 
TCTTTCAAAT GTGTGAACAG CCTCAAGGAA GATCACAACC AGCCATTGTT TGGAGTTCAG 
TTTAACTGGC ACAGTAAAGA AGGAGATCCT CTGGTGTTTG CAACTGTGGG AAGCAACAGA 
GTAACCTTAT ATGAATGCCA TTCTCAAGGG GAAATACGGT TATTGCAGTC CTATGTAGAT 
GCTGATGCAG ATGAAAACTT TTACACTTGT GCATGGACCT ATGATAGCAA CACCAGCCAC 
CCTCTGCTAG CAGTTGCTGG ATCTAGAGGC ATAATAAGAA TAATTAATCC TATAACAATG 
CAGTGTATAA AGCACTATGT TGGCCATGGG AATGCTATCA ATGAGCTGAA ATTCCACCCA 
CGAGACCCAA ACCTTCTCCT GTCAGTAAGT AAAGATCATG CTTTACGATT ATGGAATATC 
CAAACAGACA CTCTTGTGGC AATATTCGGA GGTGTGGAAG GGCACAGAGA TGAAGTGCTG 
AGTGCTGATT ATGATCTTTT GGGTGAAAAA ATAATGTCCT GTGGTATGGA TCACTCTCTT 
AAACTGTGGA GAATCAACTC AAAGAGAATG ATGAATGCGA TTAAGGAATC TTACGATTAT 
AACCCAAACA AAACTAACAG GCCATTTATT TCCCAGAAAA TCCACTTTCC TGATTTTTCT 
ACCAGAGACA TACATAGGAA TTATGTTGAT TGTGTACGAT GGTTAGGCGA TTTGATACTT 
TCCAAGTCTT GTGAAAATGC CATTGTATGC TGGAAACCTG GCAAAATGGA GGATGATATA 
GATAAAATTA AACCTAATGC TTGCATTGGG CAATCAGGTT GGCAAACTGT ATGTTTGGGA 
TTTAGAAGTA GAAGATCCTC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in genes encoding polycomb repressive complex 2 subunits cause Weaver syndrome.
Human mutation38(6)637-648(2017 Jun)
Imagawa E,Higashimoto K,Sakai Y,Numakura C,Okamoto N,Matsunaga S,Ryo A,Sato Y,Sanefuji M,Ihara K,Takada Y,Nishimura G,Saitsu H,Mizuguchi T,Miyatake S,Nakashima M,Miyake N,Soejima H,Matsumoto N

SET for life: biochemical activities and biological functions of SET domain-containing proteins.
Trends in biochemical sciences38(12)621-39(2013 Dec)
Herz HM,Garruss A,Shilatifard A

Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors.
Genes & development27(20)2221-6(2013 Oct)
Wajapeyee N,Malonia SK,Palakurthy RK,Green MR

Early activation of nSMase2/ceramide pathway in astrocytes is involved in ischemia-associated neuronal damage via inflammation in rat hippocampi.
Journal of neuroinflammation10109(2013 Sep)
Gu L,Huang B,Shen W,Gao L,Ding Z,Wu H,Guo J

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Molecular cell49(5)808-24(2013 Mar)
Simon JA,Kingston RE

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Eed cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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