FIE cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	FIE
Entrez Gene ID	821622
Full Name	Transducin/WD40 repeat-like superfamily protein
Synonyms	FERTILIZATION-INDEPENDENT ENDOSPERM,FERTILIZATION-INDEPENDENT ENDOSPERM 1,FIE1,FIS3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_112965.1	NP_188710.1	Transducin/WD40 repeat-like superfamily protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EED	NP_001026547.1
Homo sapiens (human)	EED	NP_003788.2
Canis lupus familiaris (dog)	EED	XP_005633449.1
Danio rerio (zebrafish)	eed	NP_001017766.1
	EED	XP_001102402.1
Mus musculus (house mouse)	Eed	NP_068676.1
Drosophila melanogaster (fruit fly)	escl	NP_723702.1
Arabidopsis thaliana (thale cress)	FIE	NP_188710.1
Oryza sativa (rice)	Os08g0137100	NP_001060953.1
Bos taurus (cattle)	EED	NP_001035584.1
Drosophila melanogaster (fruit fly)	esc	NP_477431.1
Xenopus tropicalis (tropical clawed frog)	eed	NP_001017325.1
Rattus norvegicus (Norway rat)	Eed	NP_001099748.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Arabidopsis FIE binding sites tend to be in gene coding regions and co-localize with H3K27me3.
Title: Arabidopsis Polycomb Repressive Complex 2 binding sites contain putative GAGA factor binding motifs within coding regions of genes.

H3K27me3 deposition is abolished genome-wide in fertilization independent endosperm protein mutants demonstrating the essential function of polycomb repressive complex 2
Title: Polycomb repressive complex 2 controls the embryo-to-seedling phase transition.

Used to demonstrate the feasibility of bimolecular fluorescence complementation in plants.
Title: Detection of protein-protein interactions in plants using bimolecular fluorescence complementation.

The following FIE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FIE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb11958	NM_112965.1 Latest version!	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (FIE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FIE ( NM_112965.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_188710.1 Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (FIE), mRNA.

Price & Availability↑

CloneID	OAb11958
Clone ID Related Accession (Same CDS sequence)	NM_112965.1
Accession Version	NM_112965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transducin/WD40 repeat-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGTCGAAGA TAACCTTAGG GAACGAGTCA ATAGTTGGGT CTTTGACTCC ATCGAATAAG 
AAATCGTACA AAGTGACGAA TAGGATTCAG GAAGGGAAGA AACCTTTGTA TGCTGTTGTT 
TTCAACTTCC TTGATGCTCG TTTCTTCGAT GTCTTCGTTA CCGCTGGTGG AAATCGGATT 
ACTCTGTACA ATTGTCTCGG AGATGGTGCC ATATCAGCAT TGCAATCCTA TGCTGATGAA 
GATAAGGAAG AGTCGTTTTA CACGGTAAGT TGGGCGTGTG GCGTTAATGG GAACCCATAT 
GTTGCGGCTG GAGGAGTAAA AGGTATAATC CGAGTCATTG ACGTCAACAG TGAAACGATT 
CATAAGAGTC TTGTGGGTCA TGGAGATTCA GTGAACGAAA TCAGGACACA ACCTTTAAAA 
CCTCAACTTG TGATTACTGC TAGCAAGGAT GAATCTGTTC GTTTGTGGAA TGTTGAAACT 
GGGATATGTA TTTTGATATT TGCTGGAGCT GGAGGTCATC GCTATGAAGT TCTAAGTGTG 
GATTTTCATC CGTCTGATAT TTACCGCTTT GCTAGTTGTG GTATGGACAC CACTATTAAA 
ATATGGTCAA TGAAAGAGTT TTGGACGTAC GTCGAGAAGT CATTCACATG GACTGATGAT 
CCATCAAAAT TCCCCACAAA ATTTGTCCAA TTCCCTGTAT TTACAGCTTC CATTCATACA 
AATTATGTAG ATTGTAACCG TTGGTTTGGT GATTTTATCC TCTCAAAGAG TGTGGACAAC 
GAGATCCTGT TGTGGGAACC ACAACTGAAA GAGAATTCTC CTGGCGAGGG AGCTTCAGAT 
GTTCTATTAA GATACCCGGT TCCAATGTGT GATATTTGGT TTATCAAGTT TTCTTGTGAC 
CTCCATTTAA GTTCTGTTGC GATAGGTAAT CAGGAAGGAA AGGTTTATGT CTGGGATTTG 
AAAAGTTGCC CTCCTGTTTT GATTACAAAG TTATCACACA ATCAATCAAA GTCTGTAATC 
AGGCAAACAG CCATGTCTGT CGATGGAAGC ACGATTCTTG CTTGCTGCGA GGACGGGACT 
ATATGGCGCT GGGACGTGAT TACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_188710.1
CDS	199..1308
Translation	MSKITLGNESIVGSLTPSNKKSYKVTNRIQEGKKPLYAVVFNFLDARFFDVFVTAGGNRITLYNCLGDGAISALQSYADEDKEESFYTVSWACGVNGNPYVAAGGVKGIIRVIDVNSETIHKSLVGHGDSVNEIRTQPLKPQLVITASKDESVRLWNVETGICILIFAGAGGHRYEVLSVDFHPSDIYRFASCGMDTTIKIWSMKEFWTYVEKSFTWTDDPSKFPTKFVQFPVFTASIHTNYVDCNRWFGDFILSKSVDNEILLWEPQLKENSPGEGASDVLLRYPVPMCDIWFIKFSCDLHLSSVAIGNQEGKVYVWDLKSCPPVLITKLSHNQSKSVIRQTAMSVDGSTILACCEDGTIWRWDVITK

Target ORF information:

RefSeq Version	NM_112965.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (FIE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_112965.1

ORF Insert Sequence:

ATGTCGAAGA TAACCTTAGG GAACGAGTCA ATAGTTGGGT CTTTGACTCC ATCGAATAAG 
AAATCGTACA AAGTGACGAA TAGGATTCAG GAAGGGAAGA AACCTTTGTA TGCTGTTGTT 
TTCAACTTCC TTGATGCTCG TTTCTTCGAT GTCTTCGTTA CCGCTGGTGG AAATCGGATT 
ACTCTGTACA ATTGTCTCGG AGATGGTGCC ATATCAGCAT TGCAATCCTA TGCTGATGAA 
GATAAGGAAG AGTCGTTTTA CACGGTAAGT TGGGCGTGTG GCGTTAATGG GAACCCATAT 
GTTGCGGCTG GAGGAGTAAA AGGTATAATC CGAGTCATTG ACGTCAACAG TGAAACGATT 
CATAAGAGTC TTGTGGGTCA TGGAGATTCA GTGAACGAAA TCAGGACACA ACCTTTAAAA 
CCTCAACTTG TGATTACTGC TAGCAAGGAT GAATCTGTTC GTTTGTGGAA TGTTGAAACT 
GGGATATGTA TTTTGATATT TGCTGGAGCT GGAGGTCATC GCTATGAAGT TCTAAGTGTG 
GATTTTCATC CGTCTGATAT TTACCGCTTT GCTAGTTGTG GTATGGACAC CACTATTAAA 
ATATGGTCAA TGAAAGAGTT TTGGACGTAC GTCGAGAAGT CATTCACATG GACTGATGAT 
CCATCAAAAT TCCCCACAAA ATTTGTCCAA TTCCCTGTAT TTACAGCTTC CATTCATACA 
AATTATGTAG ATTGTAACCG TTGGTTTGGT GATTTTATCC TCTCAAAGAG TGTGGACAAC 
GAGATCCTGT TGTGGGAACC ACAACTGAAA GAGAATTCTC CTGGCGAGGG AGCTTCAGAT 
GTTCTATTAA GATACCCGGT TCCAATGTGT GATATTTGGT TTATCAAGTT TTCTTGTGAC 
CTCCATTTAA GTTCTGTTGC GATAGGTAAT CAGGAAGGAA AGGTTTATGT CTGGGATTTG 
AAAAGTTGCC CTCCTGTTTT GATTACAAAG TTATCACACA ATCAATCAAA GTCTGTAATC 
AGGCAAACAG CCATGTCTGT CGATGGAAGC ACGATTCTTG CTTGCTGCGA GGACGGGACT 
ATATGGCGCT GGGACGTGAT TACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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FIE cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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