esc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	esc
Entrez Gene ID	34595
Full Name	extra sexcombs
Synonyms	CG14941,Dmel\CG14941,ESC,Esc,L41867,dESC,sc
General protein information	Preferred Names extra sexcombs Names CG14941 gene product from transcript CG14941-RA CG14941-PA esc-PA extra Sex combs extra sex comb
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33A2-33A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_058083.4	NP_477431.1	extra sexcombs

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EED	NP_001026547.1
Homo sapiens (human)	EED	NP_003788.2
Canis lupus familiaris (dog)	EED	XP_005633449.1
Danio rerio (zebrafish)	eed	NP_001017766.1
	EED	XP_001102402.1
Mus musculus (house mouse)	Eed	NP_068676.1
Drosophila melanogaster (fruit fly)	escl	NP_723702.1
Arabidopsis thaliana (thale cress)	FIE	NP_188710.1
Oryza sativa (rice)	Os08g0137100	NP_001060953.1
Bos taurus (cattle)	EED	NP_001035584.1
Drosophila melanogaster (fruit fly)	esc	NP_477431.1
Xenopus tropicalis (tropical clawed frog)	eed	NP_001017325.1
Rattus norvegicus (Norway rat)	Eed	NP_001099748.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ESC is a key component of the Polycomb repressive complex 2 (PRC2), which mediates H3K27me3 modification.
Title: The polycomb group mutant esc leads to augmented levels of paused Pol II in the Drosophila embryo.

analysis of the roles of ESC and ESCL in Polycomb Group mechanisms
Title: ESC, ESCL and their roles in Polycomb Group mechanisms.

These data thus indicate that ESC and ESCL play similar if not identical functions in E(Z) complexes in vivo.
Title: Drosophila ESC-like can substitute for ESC and becomes required for Polycomb silencing if ESC is absent.

A Pcl-PSC2 complex is the histone methyltransferase that generates the high levels of histone3-lysine27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state.
Title: Pcl-PRC2 is needed to generate high levels of H3-K27 trimethylation at Polycomb target genes.

one of the roles of ESC (and ESCL and EED) in Polycomb repressive complex 2 complexes is to enable Enhancer of zeste to utilize histone H3 as a substrate by physically linking enzyme and substrate
Title: The N terminus of Drosophila ESC binds directly to histone H3 and is required for E(Z)-dependent trimethylation of H3 lysine 27.

The following esc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the esc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14473	NM_058083.4 Latest version!	Drosophila melanogaster extra sexcombs (esc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

esc ( NM_058083.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477431.1 Drosophila melanogaster extra sexcombs (esc), mRNA.

Price & Availability↑

CloneID	OFa14473
Clone ID Related Accession (Same CDS sequence)	NM_058083.4
Accession Version	NM_058083.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extra sexcombs
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058083.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGTG ATAAAGTGAA AAACGGCAAC GAGCCCGAGG AGTCGGAGGA ATCATGCGGC 
GATGAGTCGG CCAGCTATAC CACCAACAGC ACCACCTCGC GCAGCAAGTC CCCGTCATCC 
AGCACGCGCA GCAAGCGCCG CGGTCGCCGG AGCACCAAAT CGAAGCCAAA AAGTCGCGCC 
GCCTACAAAT ACGACACCCA CGTGAAGGAG AACCACGGAG CCAACATCTT CGGCGTAGCC 
TTCAACACGC TGCTGGGCAA GGATGAGCCA CAGGTCTTCG CTACGGCGGG CAGCAACCGG 
GTGACCGTCT ACGAGTGCCC TCGACAGGGC GGGATGCAGC TGCTCCACTG CTATGCAGAT 
CCGGATCCCG ATGAAGTATT TTACACCTGC GCCTGGTCGT ACGACCTTAA GACCTCTTCA 
CCTCTCCTGG CCGCTGCTGG ATACCGCGGA GTCATCCGGG TTATTGATGT CGAGCAGAAT 
GAGGCGGTGG GCAACTACAT TGGCCATGGA CAGGCCATTA ACGAGCTGAA GTTCCATCCG 
CACAAGCTAC AGCTTTTGCT TTCGGGCAGC AAGGATCACG CGATAAGATT GTGGAACATC 
CAGAGCCATG TGTGCATTGC CATCCTGGGC GGAGTGGAGG GACATCGCGA TGAGGTGCTG 
TCCATCGACT TTAATATGCG TGGAGATCGC ATTGTGTCGA GCGGCATGGA TCACTCGCTG 
AAGCTGTGGT GTCTGAATAC GCCCGAGTTC CATCACAAGA TCGAACTGTC GAACACTTTC 
AGCCAGGAGA AGTCAACGCT GCCTTTCCCC ACGGTGACCA AGCACTTCCC CGATTTTTCA 
ACGCGGGATA TACACAGGAA CTATGTGGAC TGTGTGCAGT GGTTCGGCAA CTTTGTGCTC 
TCCAAGTCCT GCGAAAATGC CATTGTCTGC TGGAAACCGG GACAACTACA TCAGAGCTTC 
GAGCAGGTGA AGCCGAGCGA CTCCTCCTGC ACGATAATTG CGGAATTCGA GTACGACGAG 
TGCGAGATCT GGTTCGTGAG ATTCGGCTTC AATCCGTGGC AGAAGGTGAT TGCCCTGGGC 
AATCAGCAGG GCAAGGTGTA TGTGTGGGAG CTGGATCCCA GCGATCCCGA GGGCGCACAC 
ATGACCACCC TGCACAATTC GCGTAGCGTG GCCACTGTGC GCCAGATAGC CTTCTCTCGC 
GATGCCAGCG TACTGGTCTA CGTGTGCGAT GACGCCACCG TGTGGCGATG GAACCGCAGA 
CAAACAACTT CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477431.1
CDS	171..1448
Translation	MSSDKVKNGNEPEESEESCGDESASYTTNSTTSRSKSPSSSTRSKRRGRRSTKSKPKSRAAYKYDTHVKENHGANIFGVAFNTLLGKDEPQVFATAGSNRVTVYECPRQGGMQLLHCYADPDPDEVFYTCAWSYDLKTSSPLLAAAGYRGVIRVIDVEQNEAVGNYIGHGQAINELKFHPHKLQLLLSGSKDHAIRLWNIQSHVCIAILGGVEGHRDEVLSIDFNMRGDRIVSSGMDHSLKLWCLNTPEFHHKIELSNTFSQEKSTLPFPTVTKHFPDFSTRDIHRNYVDCVQWFGNFVLSKSCENAIVCWKPGQLHQSFEQVKPSDSSCTIIAEFEYDECEIWFVRFGFNPWQKVIALGNQQGKVYVWELDPSDPEGAHMTTLHNSRSVATVRQIAFSRDASVLVYVCDDATVWRWNRRQTTSI

Target ORF information:

RefSeq Version	NM_058083.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extra sexcombs (esc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058083.4

ORF Insert Sequence:

ATGAGCAGTG ATAAAGTGAA AAACGGCAAC GAGCCCGAGG AGTCGGAGGA ATCATGCGGC 
GATGAGTCGG CCAGCTATAC CACCAACAGC ACCACCTCGC GCAGCAAGTC CCCGTCATCC 
AGCACGCGCA GCAAGCGCCG CGGTCGCCGG AGCACCAAAT CGAAGCCAAA AAGTCGCGCC 
GCCTACAAAT ACGACACCCA CGTGAAGGAG AACCACGGAG CCAACATCTT CGGCGTAGCC 
TTCAACACGC TGCTGGGCAA GGATGAGCCA CAGGTCTTCG CTACGGCGGG CAGCAACCGG 
GTGACCGTCT ACGAGTGCCC TCGACAGGGC GGGATGCAGC TGCTCCACTG CTATGCAGAT 
CCGGATCCCG ATGAAGTATT TTACACCTGC GCCTGGTCGT ACGACCTTAA GACCTCTTCA 
CCTCTCCTGG CCGCTGCTGG ATACCGCGGA GTCATCCGGG TTATTGATGT CGAGCAGAAT 
GAGGCGGTGG GCAACTACAT TGGCCATGGA CAGGCCATTA ACGAGCTGAA GTTCCATCCG 
CACAAGCTAC AGCTTTTGCT TTCGGGCAGC AAGGATCACG CGATAAGATT GTGGAACATC 
CAGAGCCATG TGTGCATTGC CATCCTGGGC GGAGTGGAGG GACATCGCGA TGAGGTGCTG 
TCCATCGACT TTAATATGCG TGGAGATCGC ATTGTGTCGA GCGGCATGGA TCACTCGCTG 
AAGCTGTGGT GTCTGAATAC GCCCGAGTTC CATCACAAGA TCGAACTGTC GAACACTTTC 
AGCCAGGAGA AGTCAACGCT GCCTTTCCCC ACGGTGACCA AGCACTTCCC CGATTTTTCA 
ACGCGGGATA TACACAGGAA CTATGTGGAC TGTGTGCAGT GGTTCGGCAA CTTTGTGCTC 
TCCAAGTCCT GCGAAAATGC CATTGTCTGC TGGAAACCGG GACAACTACA TCAGAGCTTC 
GAGCAGGTGA AGCCGAGCGA CTCCTCCTGC ACGATAATTG CGGAATTCGA GTACGACGAG 
TGCGAGATCT GGTTCGTGAG ATTCGGCTTC AATCCGTGGC AGAAGGTGAT TGCCCTGGGC 
AATCAGCAGG GCAAGGTGTA TGTGTGGGAG CTGGATCCCA GCGATCCCGA GGGCGCACAC 
ATGACCACCC TGCACAATTC GCGTAGCGTG GCCACTGTGC GCCAGATAGC CTTCTCTCGC 
GATGCCAGCG TACTGGTCTA CGTGTGCGAT GACGCCACCG TGTGGCGATG GAACCGCAGA 
CAAACAACTT CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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esc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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