Kif21b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kif21b
Entrez Gene ID	289397
Full Name	kinesin family member 21B
General protein information	Preferred Names kinesin family member 21B Names kinesin-like protein KIF21B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006249855.3	XP_006249917.1	kinesin-like protein KIF21B isoform X3
XM_006249854.3	XP_006249916.1	kinesin-like protein KIF21B isoform X2
XM_006249857.3	XP_006249919.1	kinesin-like protein KIF21B isoform X5
XM_006249853.3	XP_006249915.1	kinesin-like protein KIF21B isoform X1
XM_006249856.3	XP_006249918.1	kinesin-like protein KIF21B isoform X4
XM_006249858.3	XP_006249920.1	kinesin-like protein KIF21B isoform X6
NM_001105990.1	NP_001099460.1	kinesin-like protein KIF21B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KIF21B	XP_004934872.1
Danio rerio (zebrafish)	kif21b	XP_699081.6
Homo sapiens (human)	KIF21B	NP_001239029.1
Rattus norvegicus (Norway rat)	Kif21b	NP_001099460.1
Pan troglodytes (chimpanzee)	KIF21B	XP_003308718.2
Xenopus tropicalis (tropical clawed frog)	kif21b	XP_004910777.1
Canis lupus familiaris (dog)	KIF21B	XP_547366.4
Mus musculus (house mouse)	Kif21b	NP_001034561.1

Gene Ontology
Bibliography

Related articles in PubMed

Activity-Dependent Regulation of Distinct Transport and Cytoskeletal Remodeling Functions of the Dendritic Kinesin KIF21B.
Ghiretti AE, Thies E, Tokito MK, Lin T, Ostap EM, Kneussel M, Holzbaur ELF
Neuron92(4)857-872(2016 Nov)

Three novel mutations in KIF21A highlight the importance of the third coiled-coil stalk domain in the etiology of CFEOM1.
Chan WM, Andrews C, Dragan L, Fredrick D, Armstrong L, Lyons C, Geraghty MT, Hunter DG, Yazdani A, Traboulsi EI, Pott JW, Gutowski NJ, Ellard S, Young E, Hanisch F, Koc F, Schnall B, Engle EC
BMC genetics826(2007 May)

Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).
Yamada K, Andrews C, Chan WM, McKeown CA, Magli A, de Berardinis T, Loewenstein A, Lazar M, O'Keefe M, Letson R, London A, Ruttum M, Matsumoto N, Saito N, Morris L, Del Monte M, Johnson RH, Uyama E, Houtman WA, de Vries B, Carlow TJ, Hart BL, Krawiecki N, Shoffner J, Vogel MC, Katowitz J, Goldstein SM, Levin AV, Sener EC, Ozturk BT, Akarsu AN, Brodsky MC, Hanisch F, Cruse RP, Zubcov AA, Robb RM, Roggenk?emper P, Gottlob I, Kowal L, Battu R, Traboulsi EI, Franceschini P, Newlin A, Demer JL, Engle EC
Nature genetics35(4)318-21(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KIF21B is a molecular regulator of activity-dependent trafficking and microtubule dynamicity in dendrites.
Title: Activity-Dependent Regulation of Distinct Transport and Cytoskeletal Remodeling Functions of the Dendritic Kinesin KIF21B.

The following Kif21b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kif21b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04420	NM_001105990.1 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35356	XM_006249853.3 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35357	XM_006249854.3 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35358	XM_006249855.3 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35359	XM_006249856.3 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35360	XM_006249857.3 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa35361	XM_006249858.3 Latest version!	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kif21b ( NM_001105990.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099460.1 Rattus norvegicus kinesin family member 21B (Kif21b), mRNA.

Price & Availability↑

CloneID	ORa04420
Clone ID Related Accession (Same CDS sequence)	NM_001105990.1
Accession Version	NM_001105990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473958.2. On or before Oct 4, 2007 this sequence version replaced XM_001063838.1, XM_223090.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAGTCTGA TGACAGCGAC TCCTCTTTGT CGGAGGTCCT GAGAGGCATC 
ATCACCCCCA TTGGCGGAGC AAAGGGAGCG CGGACGGCCC CGTTGCAGTG TGTCTCCATG 
GCCGAGGGCC ACACCAAGCC CATCCTCTGC CTGGATGCCA CCGATGAATT ACTTTTCACT 
GGATCCAAAG ACCGTAGCTG CAAGATGTGG AACCTGGTAA CAGGGCAGGA GATTGCAGCT 
CTCAAGGGCC ACCCCAACAA CGTGGTTTCT ATCAAATACT GCAGCCACTC AGGGCTGGTG 
TTCTCTGTGT CCAGCTCCTA CGTCAAGGTG TGGGACATCC GGGACTCAGC CAAGTGCATC 
CGGACGCTCA CGTCCTCAGG CCAGGTGATC TCAGGAGATG CTTGCATGGC CACATCCACC 
CGTGCCATCA CCAGTTCCCA GGGAGAGCAT CAGATTAACC AGATGGCCCT CAGCCCCTCG 
GGCACCATGT TATATGTTGC CTCCGGCAAC GCTGTCCGCA TCTGGGAGCT CAACAGGTTC 
CAGCCCATTG GCAAACTGAC CGGCCACATT GGCCCAGTGA TGTGCCTGAC AGTCACCCAG 
ACTTCAAACC AGCATGACCT CGTGGTGACT GGCTCCAAGG ACCACTATGT GAAGATGTTC 
CAGCTGGGCG ACTGTGTAAC GGGCACCATT GGCCCAACCC ACAACTTTGA GCCCCCTCAC 
TATGACGGTA TCGAATGCCT GGCCATCCAA GGAGATATAC TGTTCAGTGG TTCCAGAGAC 
AATGGCATCA AGAAGTGGGA CCTGGATCAG CAGGAGCTCA TTCAGCAAAT CCCCAACGCT 
CACAAGGACT GGGTGTGCGC CCTGGCCTTT GTACCCGGCC GGCCCATGTT ACTGAGCGCC 
TGCCGGGCAG GCTTCATCAA GGTCTGGAAT GTGGACAACT TCACGCCCAT TGGTGAGATC 
AAGGGCCATG ACAGCCCCAT CAATGCCATC TGCACCAACA GCAAGCACAT CTTCACGGCC 
TCCAGTGACT GCCGGGTAAA GCTGTGGAAT TACGTCCCTG GACTCACCCC CTGCCTTCCT 
CGACGAGTCC TGGCCATAAA GGGCCGCGCC ACCACCCTGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099460.1
CDS	302..5206
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKSDDSDSSLSEVLRGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATTLP

Target ORF information:

RefSeq Version	NM_001105990.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105990.1

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAGTCTGA TGACAGCGAC TCCTCTTTGT CGGAGGTCCT GAGAGGCATC 
ATCACCCCCA TTGGCGGAGC AAAGGGAGCG CGGACGGCCC CGTTGCAGTG TGTCTCCATG 
GCCGAGGGCC ACACCAAGCC CATCCTCTGC CTGGATGCCA CCGATGAATT ACTTTTCACT 
GGATCCAAAG ACCGTAGCTG CAAGATGTGG AACCTGGTAA CAGGGCAGGA GATTGCAGCT 
CTCAAGGGCC ACCCCAACAA CGTGGTTTCT ATCAAATACT GCAGCCACTC AGGGCTGGTG 
TTCTCTGTGT CCAGCTCCTA CGTCAAGGTG TGGGACATCC GGGACTCAGC CAAGTGCATC 
CGGACGCTCA CGTCCTCAGG CCAGGTGATC TCAGGAGATG CTTGCATGGC CACATCCACC 
CGTGCCATCA CCAGTTCCCA GGGAGAGCAT CAGATTAACC AGATGGCCCT CAGCCCCTCG 
GGCACCATGT TATATGTTGC CTCCGGCAAC GCTGTCCGCA TCTGGGAGCT CAACAGGTTC 
CAGCCCATTG GCAAACTGAC CGGCCACATT GGCCCAGTGA TGTGCCTGAC AGTCACCCAG 
ACTTCAAACC AGCATGACCT CGTGGTGACT GGCTCCAAGG ACCACTATGT GAAGATGTTC 
CAGCTGGGCG ACTGTGTAAC GGGCACCATT GGCCCAACCC ACAACTTTGA GCCCCCTCAC 
TATGACGGTA TCGAATGCCT GGCCATCCAA GGAGATATAC TGTTCAGTGG TTCCAGAGAC 
AATGGCATCA AGAAGTGGGA CCTGGATCAG CAGGAGCTCA TTCAGCAAAT CCCCAACGCT 
CACAAGGACT GGGTGTGCGC CCTGGCCTTT GTACCCGGCC GGCCCATGTT ACTGAGCGCC 
TGCCGGGCAG GCTTCATCAA GGTCTGGAAT GTGGACAACT TCACGCCCAT TGGTGAGATC 
AAGGGCCATG ACAGCCCCAT CAATGCCATC TGCACCAACA GCAAGCACAT CTTCACGGCC 
TCCAGTGACT GCCGGGTAAA GCTGTGGAAT TACGTCCCTG GACTCACCCC CTGCCTTCCT 
CGACGAGTCC TGGCCATAAA GGGCCGCGCC ACCACCCTGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21b ( XM_006249853.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249915.1 Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X1, mRNA.

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CloneID	ORa35356
Clone ID Related Accession (Same CDS sequence)	XM_006249853.3
Accession Version	XM_006249853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCA GCCCGGAGAA AGAAGCCTTC AAAAAGAGGG CAAAACTCCA GCAGGAAAAC 
AGTGAGGAGA CGGATGAGAA CGAGGCTGAG GAGGAGGAAG AAGAACGAGA TGAGAGTGGC 
TGTGAGGAGG AGGAGGATGA AGACTCGGGC AGTGAAGAGA GCCTGGTGGA CTCGGACTCC 
GACCCTGAGG AAAAGGAAGT CAACTTCCAG GCAGACCTGG CGGACCTGAC CTGTGAGATT 
GAGATCAAGC AGAAGCTGAT CGACGAGCTG GAGAACAGCC AGCGGCGGCT ACAGACGCTC 
AAGCACCAGT ACGAGGAGAA GCTCATTCTG CTGCAGAACA AGATCCGAGA CACACAGCTG 
GAGCGCGACC GCGTGCTGCA GAACCTCAGT ACTATGGAGT GCTACACGGA GGAGAAGGCC 
AACAAGATAA AGGCAGATTA TGAGAAGAGG CTTCGGGAGA TGAACCGGGA CCTGCAGAAG 
CTACAGGCTG CTCAAAAGGA GCATGCCAGG CTGCTCAAGA ACCAGTCTCG CTATGAGAGG 
GAGCTGAAGA AGCTGCAAAC GGAGGTAGCC GAAATGAAGA AGGCCAAGGT AGCCCTCATG 
AAGCAAATGC GTGAGGAACA GCAGCGGCGG CGACTGGTGG AGACCAAGAG GAACCGGGAG 
ATTGCTCAGC TCAAGAAGGA GCAACGGAGG CAGGAGTTCC AGATACGGGC CCTGGAATCT 
CAGAAGCGCC AGCAGGAAAT AGTTCTGAGG AGGAAAACCC AGGAGGTTTC CGCACTGAGG 
CGCTTAGCAA AGCCCATGTC TGAGAGAGTG GCCGGACGTG TAGGATTGAA GCCCCCCAAT 
ATGGATTCTG GGGCTGAGGT GTCTGCCAGC ACTACCTCAT CTGAGGCTGA ATCCGGAGCT 
CGCTCTGTCT CCAGCATAGT GCGTCAGTGG AACCGAAAAA TCAACCACTT CCTGGGGGAC 
CACCCGACAG CCACTGTCAA TGGCGCCCGC CCGGCCAGGA AAAAGTTCCA AAAGAAGGGA 
GCCAGCCAAA GCTTCAGTAA GGCCGCGAGA CTCAAGTGGC AGTCCCTGGA ACGGAGGATC 
ATTGACATTG TCATGCAGAG AATGACCATC GTCAACCTGG AGGCTGATAT GGAGCGTCTC 
ATAAAGAAAA GGGAGGAGCT CTTCCTCCTA CAGGAGGCAC TGCGGAGGAA GCGGGAGCGC 
CTGCAAGCTG AGAGCCCGGA GGAGGAGAAG GGACTACAGG AACTGGCTGA GGAGATCGAA 
GTGTTGGCAG CCAATATTGA CTACATCAAT GACAGCATCA CTGACTGCCA AGCCACTATC 
ATGCAGCTGG AGGAAACCAA GGAGGAGCTG GATTCGACAG ACACATCGGT GGTCATTAGT 
TCCTGCTCTC TGGCTGAAGC CCGCCTACTG CTAGACAACT TCCTCAAGGC ATCCATTGAC 
AAGGGACTTC AAGTAGCACA GAAGGAGGCC CAGATCCGGC TGCTGGAAGG ACGCCTCAGA 
CAAACAGACA TGACAGGCTC TTCCCAGAAC CACCTGCTCC TAGATGCTTT GCGTGAGAAG 
GCTGAGGCAC ACCCCGAGCT GCAGGCCCTC ATCTACAATG TGCAACATGA GAATGGCTAT 
GCCAGCACAG ATGAGGAAGT TTCAGAGTTC TCTGAGGGGA GCTTTTCCCA GTCATTCACC 
ATGAAAGGCT CCACCAGCCA TGATGACTTC AAGTTCAAGG GTGAGCCCAA GCTGTCCGCA 
CAAATGAAGG CCGTGTCTGC GGAGTGCCTA GGACCCCCAC TGGACAGCTC CACCAAGAAC 
ATCACCAAGT CCCTGGCCTC CCTCGTTGAG ATCAAAGAGG ATGGGGTGGG CTTCTCTATC 
CGGGACCCAT ACTACCGAGA CAAGGTCTCA CGCACCGTCA GCCTGCCCAC CCGGGGCAGC 
ACTTTCCCTC GGCAGTCTCG AGGTGCCACG GATACATCCC CTCTGACCCG CAGGAAGTCT 
TATGACCGGG GACAGCCCAT CAGATCAACA GACATAGGAT TCACACCTCC CTCATCACCT 
CCCACTCGGC CCCGCAACGA CCGCAATGTC TTTTCTCGCC TCACCAGCAA TCAGAGCCAG 
GGCTCGGCAC TGGACAAGTC TGATGACAGC GACTCCTCTT TGTCGGAGGT CCTGAGAGGC 
ATCATCACCC CCATTGGCGG AGCAAAGGGA GCGCGGACGG CCCCGTTGCA GTGTGTCTCC 
ATGGCCGAGG GCCACACCAA GCCCATCCTC TGCCTGGATG CCACCGATGA ATTACTTTTC 
ACTGGATCCA AAGACCGTAG CTGCAAGATG TGGAACCTGG TAACAGGGCA GGAGATTGCA 
GCTCTCAAGG GCCACCCCAA CAACGTGGTT TCTATCAAAT ACTGCAGCCA CTCAGGGCTG 
GTGTTCTCTG TGTCCAGCTC CTACGTCAAG GTGTGGGACA TCCGGGACTC AGCCAAGTGC 
ATCCGGACGC TCACGTCCTC AGGCCAGGTG ATCTCAGGAG ATGCTTGCAT GGCCACATCC 
ACCCGTGCCA TCACCAGTTC CCAGGGAGAG CATCAGATTA ACCAGATGGC CCTCAGCCCC 
TCGGGCACCA TGTTATATGT TGCCTCCGGC AACGCTGTCC GCATCTGGGA GCTCAACAGG 
TTCCAGCCCA TTGGCAAACT GACCGGCCAC ATTGGCCCAG TGATGTGCCT GACAGTCACC 
CAGACTTCAA ACCAGCATGA CCTCGTGGTG ACTGGCTCCA AGGACCACTA TGTGAAGATG 
TTCCAGCTGG GCGACTGTGT AACGGGCACC ATTGGCCCAA CCCACAACTT TGAGCCCCCT 
CACTATGACG GTATCGAATG CCTGGCCATC CAAGGAGATA TACTGTTCAG TGGTTCCAGA 
GACAATGGCA TCAAGAAGTG GGACCTGGAT CAGCAGGAGC TCATTCAGCA AATCCCCAAC 
GCTCACAAGG ACTGGGTGTG CGCCCTGGCC TTTGTACCCG GCCGGCCCAT GTTACTGAGC 
GCCTGCCGGG CAGGCTTCAT CAAGGTCTGG AATGTGGACA ACTTCACGCC CATTGGTGAG 
ATCAAGGGCC ATGACAGCCC CATCAATGCC ATCTGCACCA ACAGCAAGCA CATCTTCACG 
GCCTCCAGTG ACTGCCGGGT AAAGCTGTGG AATTACGTCC CTGGACTCAC CCCCTGCCTT 
CCTCGACGAG TCCTGGCCAT AAAGGGCCGC GCCACCACCC TGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249915.1
CDS	319..5226
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKSDDSDSSLSEVLRGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATTLP

Target ORF information:

RefSeq Version	XM_006249853.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249853.3

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCA GCCCGGAGAA AGAAGCCTTC AAAAAGAGGG CAAAACTCCA GCAGGAAAAC 
AGTGAGGAGA CGGATGAGAA CGAGGCTGAG GAGGAGGAAG AAGAACGAGA TGAGAGTGGC 
TGTGAGGAGG AGGAGGATGA AGACTCGGGC AGTGAAGAGA GCCTGGTGGA CTCGGACTCC 
GACCCTGAGG AAAAGGAAGT CAACTTCCAG GCAGACCTGG CGGACCTGAC CTGTGAGATT 
GAGATCAAGC AGAAGCTGAT CGACGAGCTG GAGAACAGCC AGCGGCGGCT ACAGACGCTC 
AAGCACCAGT ACGAGGAGAA GCTCATTCTG CTGCAGAACA AGATCCGAGA CACACAGCTG 
GAGCGCGACC GCGTGCTGCA GAACCTCAGT ACTATGGAGT GCTACACGGA GGAGAAGGCC 
AACAAGATAA AGGCAGATTA TGAGAAGAGG CTTCGGGAGA TGAACCGGGA CCTGCAGAAG 
CTACAGGCTG CTCAAAAGGA GCATGCCAGG CTGCTCAAGA ACCAGTCTCG CTATGAGAGG 
GAGCTGAAGA AGCTGCAAAC GGAGGTAGCC GAAATGAAGA AGGCCAAGGT AGCCCTCATG 
AAGCAAATGC GTGAGGAACA GCAGCGGCGG CGACTGGTGG AGACCAAGAG GAACCGGGAG 
ATTGCTCAGC TCAAGAAGGA GCAACGGAGG CAGGAGTTCC AGATACGGGC CCTGGAATCT 
CAGAAGCGCC AGCAGGAAAT AGTTCTGAGG AGGAAAACCC AGGAGGTTTC CGCACTGAGG 
CGCTTAGCAA AGCCCATGTC TGAGAGAGTG GCCGGACGTG TAGGATTGAA GCCCCCCAAT 
ATGGATTCTG GGGCTGAGGT GTCTGCCAGC ACTACCTCAT CTGAGGCTGA ATCCGGAGCT 
CGCTCTGTCT CCAGCATAGT GCGTCAGTGG AACCGAAAAA TCAACCACTT CCTGGGGGAC 
CACCCGACAG CCACTGTCAA TGGCGCCCGC CCGGCCAGGA AAAAGTTCCA AAAGAAGGGA 
GCCAGCCAAA GCTTCAGTAA GGCCGCGAGA CTCAAGTGGC AGTCCCTGGA ACGGAGGATC 
ATTGACATTG TCATGCAGAG AATGACCATC GTCAACCTGG AGGCTGATAT GGAGCGTCTC 
ATAAAGAAAA GGGAGGAGCT CTTCCTCCTA CAGGAGGCAC TGCGGAGGAA GCGGGAGCGC 
CTGCAAGCTG AGAGCCCGGA GGAGGAGAAG GGACTACAGG AACTGGCTGA GGAGATCGAA 
GTGTTGGCAG CCAATATTGA CTACATCAAT GACAGCATCA CTGACTGCCA AGCCACTATC 
ATGCAGCTGG AGGAAACCAA GGAGGAGCTG GATTCGACAG ACACATCGGT GGTCATTAGT 
TCCTGCTCTC TGGCTGAAGC CCGCCTACTG CTAGACAACT TCCTCAAGGC ATCCATTGAC 
AAGGGACTTC AAGTAGCACA GAAGGAGGCC CAGATCCGGC TGCTGGAAGG ACGCCTCAGA 
CAAACAGACA TGACAGGCTC TTCCCAGAAC CACCTGCTCC TAGATGCTTT GCGTGAGAAG 
GCTGAGGCAC ACCCCGAGCT GCAGGCCCTC ATCTACAATG TGCAACATGA GAATGGCTAT 
GCCAGCACAG ATGAGGAAGT TTCAGAGTTC TCTGAGGGGA GCTTTTCCCA GTCATTCACC 
ATGAAAGGCT CCACCAGCCA TGATGACTTC AAGTTCAAGG GTGAGCCCAA GCTGTCCGCA 
CAAATGAAGG CCGTGTCTGC GGAGTGCCTA GGACCCCCAC TGGACAGCTC CACCAAGAAC 
ATCACCAAGT CCCTGGCCTC CCTCGTTGAG ATCAAAGAGG ATGGGGTGGG CTTCTCTATC 
CGGGACCCAT ACTACCGAGA CAAGGTCTCA CGCACCGTCA GCCTGCCCAC CCGGGGCAGC 
ACTTTCCCTC GGCAGTCTCG AGGTGCCACG GATACATCCC CTCTGACCCG CAGGAAGTCT 
TATGACCGGG GACAGCCCAT CAGATCAACA GACATAGGAT TCACACCTCC CTCATCACCT 
CCCACTCGGC CCCGCAACGA CCGCAATGTC TTTTCTCGCC TCACCAGCAA TCAGAGCCAG 
GGCTCGGCAC TGGACAAGTC TGATGACAGC GACTCCTCTT TGTCGGAGGT CCTGAGAGGC 
ATCATCACCC CCATTGGCGG AGCAAAGGGA GCGCGGACGG CCCCGTTGCA GTGTGTCTCC 
ATGGCCGAGG GCCACACCAA GCCCATCCTC TGCCTGGATG CCACCGATGA ATTACTTTTC 
ACTGGATCCA AAGACCGTAG CTGCAAGATG TGGAACCTGG TAACAGGGCA GGAGATTGCA 
GCTCTCAAGG GCCACCCCAA CAACGTGGTT TCTATCAAAT ACTGCAGCCA CTCAGGGCTG 
GTGTTCTCTG TGTCCAGCTC CTACGTCAAG GTGTGGGACA TCCGGGACTC AGCCAAGTGC 
ATCCGGACGC TCACGTCCTC AGGCCAGGTG ATCTCAGGAG ATGCTTGCAT GGCCACATCC 
ACCCGTGCCA TCACCAGTTC CCAGGGAGAG CATCAGATTA ACCAGATGGC CCTCAGCCCC 
TCGGGCACCA TGTTATATGT TGCCTCCGGC AACGCTGTCC GCATCTGGGA GCTCAACAGG 
TTCCAGCCCA TTGGCAAACT GACCGGCCAC ATTGGCCCAG TGATGTGCCT GACAGTCACC 
CAGACTTCAA ACCAGCATGA CCTCGTGGTG ACTGGCTCCA AGGACCACTA TGTGAAGATG 
TTCCAGCTGG GCGACTGTGT AACGGGCACC ATTGGCCCAA CCCACAACTT TGAGCCCCCT 
CACTATGACG GTATCGAATG CCTGGCCATC CAAGGAGATA TACTGTTCAG TGGTTCCAGA 
GACAATGGCA TCAAGAAGTG GGACCTGGAT CAGCAGGAGC TCATTCAGCA AATCCCCAAC 
GCTCACAAGG ACTGGGTGTG CGCCCTGGCC TTTGTACCCG GCCGGCCCAT GTTACTGAGC 
GCCTGCCGGG CAGGCTTCAT CAAGGTCTGG AATGTGGACA ACTTCACGCC CATTGGTGAG 
ATCAAGGGCC ATGACAGCCC CATCAATGCC ATCTGCACCA ACAGCAAGCA CATCTTCACG 
GCCTCCAGTG ACTGCCGGGT AAAGCTGTGG AATTACGTCC CTGGACTCAC CCCCTGCCTT 
CCTCGACGAG TCCTGGCCAT AAAGGGCCGC GCCACCACCC TGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21b ( XM_006249854.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249916.1 Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35357
Clone ID Related Accession (Same CDS sequence)	XM_006249854.3
Accession Version	XM_006249854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCA GCCCGGAGAA AGAAGCCTTC AAAAAGAGGG CAAAACTCCA GCAGGAAAAC 
AGTGAGGAGA CGGATGAGAA CGAGGCTGAG GAGGAGGAAG AAGAACGAGA TGAGAGTGGC 
TGTGAGGAGG AGGAGGATGA AGACTCGGGC AGTGAAGAGA GCCTGGTGGA CTCGGACTCC 
GACCCTGAGG AAAAGGAAGT CAACTTCCAG GCAGACCTGG CGGACCTGAC CTGTGAGATT 
GAGATCAAGC AGAAGCTGAT CGACGAGCTG GAGAACAGCC AGCGGCGGCT ACAGACGCTC 
AAGCACCAGT ACGAGGAGAA GCTCATTCTG CTGCAGAACA AGATCCGAGA CACACAGCTG 
GAGCGCGACC GCGTGCTGCA GAACCTCAGT ACTATGGAGT GCTACACGGA GGAGAAGGCC 
AACAAGATAA AGGCAGATTA TGAGAAGAGG CTTCGGGAGA TGAACCGGGA CCTGCAGAAG 
CTACAGGCTG CTCAAAAGGA GCATGCCAGG CTGCTCAAGA ACCAGTCTCG CTATGAGAGG 
GAGCTGAAGA AGCTGCAAAC GGAGGTAGCC GAAATGAAGA AGGCCAAGGT AGCCCTCATG 
AAGCAAATGC GTGAGGAACA GCAGCGGCGG CGACTGGTGG AGACCAAGAG GAACCGGGAG 
ATTGCTCAGC TCAAGAAGGA GCAACGGAGG CAGGAGTTCC AGATACGGGC CCTGGAATCT 
CAGAAGCGCC AGCAGGAAAT AGTTCTGAGG AGGAAAACCC AGGAGGTTTC CGCACTGAGG 
CGCTTAGCAA AGCCCATGTC TGAGAGAGTG GCCGGACGTG TAGGATTGAA GCCCCCCAAT 
ATGGATTCTG GGGCTGAGGT GTCTGCCAGC ACTACCTCAT CTGAGGCTGA ATCCGGAGCT 
CGCTCTGTCT CCAGCATAGT GCGTCAGTGG AACCGAAAAA TCAACCACTT CCTGGGGGAC 
CACCCGACAG CCACTGTCAA TGGCGCCCGC CCGGCCAGGA AAAAGTTCCA AAAGAAGGGA 
GCCAGCCAAA GCTTCAGTAA GGCCGCGAGA CTCAAGTGGC AGTCCCTGGA ACGGAGGATC 
ATTGACATTG TCATGCAGAG AATGACCATC GTCAACCTGG AGGCTGATAT GGAGCGTCTC 
ATAAAGAAAA GGGAGGAGCT CTTCCTCCTA CAGGAGGCAC TGCGGAGGAA GCGGGAGCGC 
CTGCAAGCTG AGAGCCCGGA GGAGGAGAAG GGACTACAGG AACTGGCTGA GGAGATCGAA 
GTGTTGGCAG CCAATATTGA CTACATCAAT GACAGCATCA CTGACTGCCA AGCCACTATC 
ATGCAGCTGG AGGAAACCAA GGAGGAGCTG GATTCGACAG ACACATCGGT GGTCATTAGT 
TCCTGCTCTC TGGCTGAAGC CCGCCTACTG CTAGACAACT TCCTCAAGGC ATCCATTGAC 
AAGGGACTTC AAGTAGCACA GAAGGAGGCC CAGATCCGGC TGCTGGAAGG ACGCCTCAGA 
CAAACAGACA TGACAGGCTC TTCCCAGAAC CACCTGCTCC TAGATGCTTT GCGTGAGAAG 
GCTGAGGCAC ACCCCGAGCT GCAGGCCCTC ATCTACAATG TGCAACATGA GAATGGCTAT 
GCCAGCACAG ATGAGGAAGT TTCAGAGTTC TCTGAGGGGA GCTTTTCCCA GTCATTCACC 
ATGAAAGGCT CCACCAGCCA TGATGACTTC AAGTTCAAGG GTGAGCCCAA GCTGTCCGCA 
CAAATGAAGG CCGTGTCTGC GGAGTGCCTA GGACCCCCAC TGGACAGCTC CACCAAGAAC 
ATCACCAAGT CCCTGGCCTC CCTCGTTGAG ATCAAAGAGG ATGGGGTGGG CTTCTCTATC 
CGGGACCCAT ACTACCGAGA CAAGGTCTCA CGCACCGTCA GCCTGCCCAC CCGGGGCAGC 
ACTTTCCCTC GGCAGTCTCG AGGTGCCACG GATACATCCC CTCTGACCCG CAGGAAGTCT 
TATGACCGGG GACAGCCCAT CAGATCAACA GACATAGGAT TCACACCTCC CTCATCACCT 
CCCACTCGGC CCCGCAACGA CCGCAATGTC TTTTCTCGCC TCACCAGCAA TCAGAGCCAG 
GGCTCGGCAC TGGACAAAGG CATCATCACC CCCATTGGCG GAGCAAAGGG AGCGCGGACG 
GCCCCGTTGC AGTGTGTCTC CATGGCCGAG GGCCACACCA AGCCCATCCT CTGCCTGGAT 
GCCACCGATG AATTACTTTT CACTGGATCC AAAGACCGTA GCTGCAAGAT GTGGAACCTG 
GTAACAGGGC AGGAGATTGC AGCTCTCAAG GGCCACCCCA ACAACGTGGT TTCTATCAAA 
TACTGCAGCC ACTCAGGGCT GGTGTTCTCT GTGTCCAGCT CCTACGTCAA GGTGTGGGAC 
ATCCGGGACT CAGCCAAGTG CATCCGGACG CTCACGTCCT CAGGCCAGGT GATCTCAGGA 
GATGCTTGCA TGGCCACATC CACCCGTGCC ATCACCAGTT CCCAGGGAGA GCATCAGATT 
AACCAGATGG CCCTCAGCCC CTCGGGCACC ATGTTATATG TTGCCTCCGG CAACGCTGTC 
CGCATCTGGG AGCTCAACAG GTTCCAGCCC ATTGGCAAAC TGACCGGCCA CATTGGCCCA 
GTGATGTGCC TGACAGTCAC CCAGACTTCA AACCAGCATG ACCTCGTGGT GACTGGCTCC 
AAGGACCACT ATGTGAAGAT GTTCCAGCTG GGCGACTGTG TAACGGGCAC CATTGGCCCA 
ACCCACAACT TTGAGCCCCC TCACTATGAC GGTATCGAAT GCCTGGCCAT CCAAGGAGAT 
ATACTGTTCA GTGGTTCCAG AGACAATGGC ATCAAGAAGT GGGACCTGGA TCAGCAGGAG 
CTCATTCAGC AAATCCCCAA CGCTCACAAG GACTGGGTGT GCGCCCTGGC CTTTGTACCC 
GGCCGGCCCA TGTTACTGAG CGCCTGCCGG GCAGGCTTCA TCAAGGTCTG GAATGTGGAC 
AACTTCACGC CCATTGGTGA GATCAAGGGC CATGACAGCC CCATCAATGC CATCTGCACC 
AACAGCAAGC ACATCTTCAC GGCCTCCAGT GACTGCCGGG TAAAGCTGTG GAATTACGTC 
CCTGGACTCA CCCCCTGCCT TCCTCGACGA GTCCTGGCCA TAAAGGGCCG CGCCACCACC 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249916.1
CDS	319..5187
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATTLP

Target ORF information:

RefSeq Version	XM_006249854.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249854.3

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCA GCCCGGAGAA AGAAGCCTTC AAAAAGAGGG CAAAACTCCA GCAGGAAAAC 
AGTGAGGAGA CGGATGAGAA CGAGGCTGAG GAGGAGGAAG AAGAACGAGA TGAGAGTGGC 
TGTGAGGAGG AGGAGGATGA AGACTCGGGC AGTGAAGAGA GCCTGGTGGA CTCGGACTCC 
GACCCTGAGG AAAAGGAAGT CAACTTCCAG GCAGACCTGG CGGACCTGAC CTGTGAGATT 
GAGATCAAGC AGAAGCTGAT CGACGAGCTG GAGAACAGCC AGCGGCGGCT ACAGACGCTC 
AAGCACCAGT ACGAGGAGAA GCTCATTCTG CTGCAGAACA AGATCCGAGA CACACAGCTG 
GAGCGCGACC GCGTGCTGCA GAACCTCAGT ACTATGGAGT GCTACACGGA GGAGAAGGCC 
AACAAGATAA AGGCAGATTA TGAGAAGAGG CTTCGGGAGA TGAACCGGGA CCTGCAGAAG 
CTACAGGCTG CTCAAAAGGA GCATGCCAGG CTGCTCAAGA ACCAGTCTCG CTATGAGAGG 
GAGCTGAAGA AGCTGCAAAC GGAGGTAGCC GAAATGAAGA AGGCCAAGGT AGCCCTCATG 
AAGCAAATGC GTGAGGAACA GCAGCGGCGG CGACTGGTGG AGACCAAGAG GAACCGGGAG 
ATTGCTCAGC TCAAGAAGGA GCAACGGAGG CAGGAGTTCC AGATACGGGC CCTGGAATCT 
CAGAAGCGCC AGCAGGAAAT AGTTCTGAGG AGGAAAACCC AGGAGGTTTC CGCACTGAGG 
CGCTTAGCAA AGCCCATGTC TGAGAGAGTG GCCGGACGTG TAGGATTGAA GCCCCCCAAT 
ATGGATTCTG GGGCTGAGGT GTCTGCCAGC ACTACCTCAT CTGAGGCTGA ATCCGGAGCT 
CGCTCTGTCT CCAGCATAGT GCGTCAGTGG AACCGAAAAA TCAACCACTT CCTGGGGGAC 
CACCCGACAG CCACTGTCAA TGGCGCCCGC CCGGCCAGGA AAAAGTTCCA AAAGAAGGGA 
GCCAGCCAAA GCTTCAGTAA GGCCGCGAGA CTCAAGTGGC AGTCCCTGGA ACGGAGGATC 
ATTGACATTG TCATGCAGAG AATGACCATC GTCAACCTGG AGGCTGATAT GGAGCGTCTC 
ATAAAGAAAA GGGAGGAGCT CTTCCTCCTA CAGGAGGCAC TGCGGAGGAA GCGGGAGCGC 
CTGCAAGCTG AGAGCCCGGA GGAGGAGAAG GGACTACAGG AACTGGCTGA GGAGATCGAA 
GTGTTGGCAG CCAATATTGA CTACATCAAT GACAGCATCA CTGACTGCCA AGCCACTATC 
ATGCAGCTGG AGGAAACCAA GGAGGAGCTG GATTCGACAG ACACATCGGT GGTCATTAGT 
TCCTGCTCTC TGGCTGAAGC CCGCCTACTG CTAGACAACT TCCTCAAGGC ATCCATTGAC 
AAGGGACTTC AAGTAGCACA GAAGGAGGCC CAGATCCGGC TGCTGGAAGG ACGCCTCAGA 
CAAACAGACA TGACAGGCTC TTCCCAGAAC CACCTGCTCC TAGATGCTTT GCGTGAGAAG 
GCTGAGGCAC ACCCCGAGCT GCAGGCCCTC ATCTACAATG TGCAACATGA GAATGGCTAT 
GCCAGCACAG ATGAGGAAGT TTCAGAGTTC TCTGAGGGGA GCTTTTCCCA GTCATTCACC 
ATGAAAGGCT CCACCAGCCA TGATGACTTC AAGTTCAAGG GTGAGCCCAA GCTGTCCGCA 
CAAATGAAGG CCGTGTCTGC GGAGTGCCTA GGACCCCCAC TGGACAGCTC CACCAAGAAC 
ATCACCAAGT CCCTGGCCTC CCTCGTTGAG ATCAAAGAGG ATGGGGTGGG CTTCTCTATC 
CGGGACCCAT ACTACCGAGA CAAGGTCTCA CGCACCGTCA GCCTGCCCAC CCGGGGCAGC 
ACTTTCCCTC GGCAGTCTCG AGGTGCCACG GATACATCCC CTCTGACCCG CAGGAAGTCT 
TATGACCGGG GACAGCCCAT CAGATCAACA GACATAGGAT TCACACCTCC CTCATCACCT 
CCCACTCGGC CCCGCAACGA CCGCAATGTC TTTTCTCGCC TCACCAGCAA TCAGAGCCAG 
GGCTCGGCAC TGGACAAAGG CATCATCACC CCCATTGGCG GAGCAAAGGG AGCGCGGACG 
GCCCCGTTGC AGTGTGTCTC CATGGCCGAG GGCCACACCA AGCCCATCCT CTGCCTGGAT 
GCCACCGATG AATTACTTTT CACTGGATCC AAAGACCGTA GCTGCAAGAT GTGGAACCTG 
GTAACAGGGC AGGAGATTGC AGCTCTCAAG GGCCACCCCA ACAACGTGGT TTCTATCAAA 
TACTGCAGCC ACTCAGGGCT GGTGTTCTCT GTGTCCAGCT CCTACGTCAA GGTGTGGGAC 
ATCCGGGACT CAGCCAAGTG CATCCGGACG CTCACGTCCT CAGGCCAGGT GATCTCAGGA 
GATGCTTGCA TGGCCACATC CACCCGTGCC ATCACCAGTT CCCAGGGAGA GCATCAGATT 
AACCAGATGG CCCTCAGCCC CTCGGGCACC ATGTTATATG TTGCCTCCGG CAACGCTGTC 
CGCATCTGGG AGCTCAACAG GTTCCAGCCC ATTGGCAAAC TGACCGGCCA CATTGGCCCA 
GTGATGTGCC TGACAGTCAC CCAGACTTCA AACCAGCATG ACCTCGTGGT GACTGGCTCC 
AAGGACCACT ATGTGAAGAT GTTCCAGCTG GGCGACTGTG TAACGGGCAC CATTGGCCCA 
ACCCACAACT TTGAGCCCCC TCACTATGAC GGTATCGAAT GCCTGGCCAT CCAAGGAGAT 
ATACTGTTCA GTGGTTCCAG AGACAATGGC ATCAAGAAGT GGGACCTGGA TCAGCAGGAG 
CTCATTCAGC AAATCCCCAA CGCTCACAAG GACTGGGTGT GCGCCCTGGC CTTTGTACCC 
GGCCGGCCCA TGTTACTGAG CGCCTGCCGG GCAGGCTTCA TCAAGGTCTG GAATGTGGAC 
AACTTCACGC CCATTGGTGA GATCAAGGGC CATGACAGCC CCATCAATGC CATCTGCACC 
AACAGCAAGC ACATCTTCAC GGCCTCCAGT GACTGCCGGG TAAAGCTGTG GAATTACGTC 
CCTGGACTCA CCCCCTGCCT TCCTCGACGA GTCCTGGCCA TAAAGGGCCG CGCCACCACC 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21b ( XM_006249855.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249917.1 Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X3, mRNA.

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CloneID	ORa35358
Clone ID Related Accession (Same CDS sequence)	XM_006249855.3
Accession Version	XM_006249855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAAGGCAT CATCACCCCC ATTGGCGGAG CAAAGGGAGC GCGGACGGCC 
CCGTTGCAGT GTGTCTCCAT GGCCGAGGGC CACACCAAGC CCATCCTCTG CCTGGATGCC 
ACCGATGAAT TACTTTTCAC TGGATCCAAA GACCGTAGCT GCAAGATGTG GAACCTGGTA 
ACAGGGCAGG AGATTGCAGC TCTCAAGGGC CACCCCAACA ACGTGGTTTC TATCAAATAC 
TGCAGCCACT CAGGGCTGGT GTTCTCTGTG TCCAGCTCCT ACGTCAAGGT GTGGGACATC 
CGGGACTCAG CCAAGTGCAT CCGGACGCTC ACGTCCTCAG GCCAGGTGAT CTCAGGAGAT 
GCTTGCATGG CCACATCCAC CCGTGCCATC ACCAGTTCCC AGGGAGAGCA TCAGATTAAC 
CAGATGGCCC TCAGCCCCTC GGGCACCATG TTATATGTTG CCTCCGGCAA CGCTGTCCGC 
ATCTGGGAGC TCAACAGGTT CCAGCCCATT GGCAAACTGA CCGGCCACAT TGGCCCAGTG 
ATGTGCCTGA CAGTCACCCA GACTTCAAAC CAGCATGACC TCGTGGTGAC TGGCTCCAAG 
GACCACTATG TGAAGATGTT CCAGCTGGGC GACTGTGTAA CGGGCACCAT TGGCCCAACC 
CACAACTTTG AGCCCCCTCA CTATGACGGT ATCGAATGCC TGGCCATCCA AGGAGATATA 
CTGTTCAGTG GTTCCAGAGA CAATGGCATC AAGAAGTGGG ACCTGGATCA GCAGGAGCTC 
ATTCAGCAAA TCCCCAACGC TCACAAGGAC TGGGTGTGCG CCCTGGCCTT TGTACCCGGC 
CGGCCCATGT TACTGAGCGC CTGCCGGGCA GGCTTCATCA AGGTCTGGAA TGTGGACAAC 
TTCACGCCCA TTGGTGAGAT CAAGGGCCAT GACAGCCCCA TCAATGCCAT CTGCACCAAC 
AGCAAGCACA TCTTCACGGC CTCCAGTGAC TGCCGGGTAA AGCTGTGGAA TTACGTCCCT 
GGACTCACCC CCTGCCTTCC TCGACGAGTC CTGGCCATAA AGGGCCGCGC CACCACCCTG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249917.1
CDS	319..5184
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDCRVKLWNYVPGLTPCLPRRVLAIKGRATTLP

Target ORF information:

RefSeq Version	XM_006249855.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249855.3

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAAGGCAT CATCACCCCC ATTGGCGGAG CAAAGGGAGC GCGGACGGCC 
CCGTTGCAGT GTGTCTCCAT GGCCGAGGGC CACACCAAGC CCATCCTCTG CCTGGATGCC 
ACCGATGAAT TACTTTTCAC TGGATCCAAA GACCGTAGCT GCAAGATGTG GAACCTGGTA 
ACAGGGCAGG AGATTGCAGC TCTCAAGGGC CACCCCAACA ACGTGGTTTC TATCAAATAC 
TGCAGCCACT CAGGGCTGGT GTTCTCTGTG TCCAGCTCCT ACGTCAAGGT GTGGGACATC 
CGGGACTCAG CCAAGTGCAT CCGGACGCTC ACGTCCTCAG GCCAGGTGAT CTCAGGAGAT 
GCTTGCATGG CCACATCCAC CCGTGCCATC ACCAGTTCCC AGGGAGAGCA TCAGATTAAC 
CAGATGGCCC TCAGCCCCTC GGGCACCATG TTATATGTTG CCTCCGGCAA CGCTGTCCGC 
ATCTGGGAGC TCAACAGGTT CCAGCCCATT GGCAAACTGA CCGGCCACAT TGGCCCAGTG 
ATGTGCCTGA CAGTCACCCA GACTTCAAAC CAGCATGACC TCGTGGTGAC TGGCTCCAAG 
GACCACTATG TGAAGATGTT CCAGCTGGGC GACTGTGTAA CGGGCACCAT TGGCCCAACC 
CACAACTTTG AGCCCCCTCA CTATGACGGT ATCGAATGCC TGGCCATCCA AGGAGATATA 
CTGTTCAGTG GTTCCAGAGA CAATGGCATC AAGAAGTGGG ACCTGGATCA GCAGGAGCTC 
ATTCAGCAAA TCCCCAACGC TCACAAGGAC TGGGTGTGCG CCCTGGCCTT TGTACCCGGC 
CGGCCCATGT TACTGAGCGC CTGCCGGGCA GGCTTCATCA AGGTCTGGAA TGTGGACAAC 
TTCACGCCCA TTGGTGAGAT CAAGGGCCAT GACAGCCCCA TCAATGCCAT CTGCACCAAC 
AGCAAGCACA TCTTCACGGC CTCCAGTGAC TGCCGGGTAA AGCTGTGGAA TTACGTCCCT 
GGACTCACCC CCTGCCTTCC TCGACGAGTC CTGGCCATAA AGGGCCGCGC CACCACCCTG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21b ( XM_006249856.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249918.1 Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X4, mRNA.

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CloneID	ORa35359
Clone ID Related Accession (Same CDS sequence)	XM_006249856.3
Accession Version	XM_006249856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCA GCCCGGAGAA AGAAGCCTTC AAAAAGAGGG CAAAACTCCA GCAGGAAAAC 
AGTGAGGAGA CGGATGAGAA CGAGGCTGAG GAGGAGGAAG AAGAACGAGA TGAGAGTGGC 
TGTGAGGAGG AGGAGGATGA AGACTCGGGC AGTGAAGAGA GCCTGGTGGA CTCGGACTCC 
GACCCTGAGG AAAAGGAAGT CAACTTCCAG GCAGACCTGG CGGACCTGAC CTGTGAGATT 
GAGATCAAGC AGAAGCTGAT CGACGAGCTG GAGAACAGCC AGCGGCGGCT ACAGACGCTC 
AAGCACCAGT ACGAGGAGAA GCTCATTCTG CTGCAGAACA AGATCCGAGA CACACAGCTG 
GAGCGCGACC GCGTGCTGCA GAACCTCAGT ACTATGGAGT GCTACACGGA GGAGAAGGCC 
AACAAGATAA AGGCAGATTA TGAGAAGAGG CTTCGGGAGA TGAACCGGGA CCTGCAGAAG 
CTACAGGCTG CTCAAAAGGA GCATGCCAGG CTGCTCAAGA ACCAGTCTCG CTATGAGAGG 
GAGCTGAAGA AGCTGCAAAC GGAGGTAGCC GAAATGAAGA AGGCCAAGGT AGCCCTCATG 
AAGCAAATGC GTGAGGAACA GCAGCGGCGG CGACTGGTGG AGACCAAGAG GAACCGGGAG 
ATTGCTCAGC TCAAGAAGGA GCAACGGAGG CAGGAGTTCC AGATACGGGC CCTGGAATCT 
CAGAAGCGCC AGCAGGAAAT AGTTCTGAGG AGGAAAACCC AGGAGGTTTC CGCACTGAGG 
CGCTTAGCAA AGCCCATGTC TGAGAGAGTG GCCGGACGTG TAGGATTGAA GCCCCCCAAT 
ATGGATTCTG GGGCTGAGGT GTCTGCCAGC ACTACCTCAT CTGAGGCTGA ATCCGGAGCT 
CGCTCTGTCT CCAGCATAGT GCGTCAGTGG AACCGAAAAA TCAACCACTT CCTGGGGGAC 
CACCCGACAG CCACTGTCAA TGGCGCCCGC CCGGCCAGGA AAAAGTTCCA AAAGAAGGGA 
GCCAGCCAAA GCTTCAGTAA GGCCGCGAGA CTCAAGTGGC AGTCCCTGGA ACGGAGGATC 
ATTGACATTG TCATGCAGAG AATGACCATC GTCAACCTGG AGGCTGATAT GGAGCGTCTC 
ATAAAGAAAA GGGAGGAGCT CTTCCTCCTA CAGGAGGCAC TGCGGAGGAA GCGGGAGCGC 
CTGCAAGCTG AGAGCCCGGA GGAGGAGAAG GGACTACAGG AACTGGCTGA GGAGATCGAA 
GTGTTGGCAG CCAATATTGA CTACATCAAT GACAGCATCA CTGACTGCCA AGCCACTATC 
ATGCAGCTGG AGGAAACCAA GGAGGAGCTG GATTCGACAG ACACATCGGT GGTCATTAGT 
TCCTGCTCTC TGGCTGAAGC CCGCCTACTG CTAGACAACT TCCTCAAGGC ATCCATTGAC 
AAGGGACTTC AAGTAGCACA GAAGGAGGCC CAGATCCGGC TGCTGGAAGG ACGCCTCAGA 
CAAACAGACA TGACAGGCTC TTCCCAGAAC CACCTGCTCC TAGATGCTTT GCGTGAGAAG 
GCTGAGGCAC ACCCCGAGCT GCAGGCCCTC ATCTACAATG TGCAACATGA GAATGGCTAT 
GCCAGCACAG ATGAGGAAGT TTCAGAGTTC TCTGAGGGGA GCTTTTCCCA GTCATTCACC 
ATGAAAGGCT CCACCAGCCA TGATGACTTC AAGTTCAAGG GTGAGCCCAA GCTGTCCGCA 
CAAATGAAGG CCGTGTCTGC GGAGTGCCTA GGACCCCCAC TGGACAGCTC CACCAAGAAC 
ATCACCAAGT CCCTGGCCTC CCTCGTTGAG ATCAAAGAGG ATGGGGTGGG CTTCTCTATC 
CGGGACCCAT ACTACCGAGA CAAGGTCTCA CGCACCGTCA GCCTGCCCAC CCGGGGCAGC 
ACTTTCCCTC GGCAGTCTCG AGGTGCCACG GATACATCCC CTCTGACCCG CAGGAAGTCT 
TATGACCGGG GACAGCCCAT CAGATCAACA GACATAGGAT TCACACCTCC CTCATCACCT 
CCCACTCGGC CCCGCAACGA CCGCAATGTC TTTTCTCGCC TCACCAGCAA TCAGAGCCAG 
GGCTCGGCAC TGGACAAGTC TGATGACAGC GACTCCTCTT TGTCGGAGGT CCTGAGAGGC 
ATCATCACCC CCATTGGCGG AGCAAAGGGA GCGCGGACGG CCCCGTTGCA GTGTGTCTCC 
ATGGCCGAGG GCCACACCAA GCCCATCCTC TGCCTGGATG CCACCGATGA ATTACTTTTC 
ACTGGATCCA AAGACCGTAG CTGCAAGATG TGGAACCTGG TAACAGGGCA GGAGATTGCA 
GCTCTCAAGG GCCACCCCAA CAACGTGGTT TCTATCAAAT ACTGCAGCCA CTCAGGGCTG 
GTGTTCTCTG TGTCCAGCTC CTACGTCAAG GTGTGGGACA TCCGGGACTC AGCCAAGTGC 
ATCCGGACGC TCACGTCCTC AGGCCAGGTG ATCTCAGGAG ATGCTTGCAT GGCCACATCC 
ACCCGTGCCA TCACCAGTTC CCAGGGAGAG CATCAGATTA ACCAGATGGC CCTCAGCCCC 
TCGGGCACCA TGTTATATGT TGCCTCCGGC AACGCTGTCC GCATCTGGGA GCTCAACAGG 
TTCCAGCCCA TTGGCAAACT GACCGGCCAC ATTGGCCCAG TGATGTGCCT GACAGTCACC 
CAGACTTCAA ACCAGCATGA CCTCGTGGTG ACTGGCTCCA AGGACCACTA TGTGAAGATG 
TTCCAGCTGG GCGACTGTGT AACGGGCACC ATTGGCCCAA CCCACAACTT TGAGCCCCCT 
CACTATGACG GTATCGAATG CCTGGCCATC CAAGGAGATA TACTGTTCAG TGGTTCCAGA 
GACAATGGCA TCAAGAAGTG GGACCTGGAT CAGCAGGAGC TCATTCAGCA AATCCCCAAC 
GCTCACAAGG ACTGGGTGTG CGCCCTGGCC TTTGTACCCG GCCGGCCCAT GTTACTGAGC 
GCCTGCCGGG CAGGCTTCAT CAAGGTCTGG AATGTGGACA ACTTCACGCC CATTGGTGAG 
ATCAAGGGCC ATGACAGCCC CATCAATGCC ATCTGCACCA ACAGCAAGCA CATCTTCACG 
GCCTCCAGTG ACCTGACAGT GAAGTTCTGG AGCATGCGGC GGCTACCAGC AGCCCACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249918.1
CDS	319..5181
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKSDDSDSSLSEVLRGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDLTVKFWSMRRLPAAHP

Target ORF information:

RefSeq Version	XM_006249856.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249856.3

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCA GCCCGGAGAA AGAAGCCTTC AAAAAGAGGG CAAAACTCCA GCAGGAAAAC 
AGTGAGGAGA CGGATGAGAA CGAGGCTGAG GAGGAGGAAG AAGAACGAGA TGAGAGTGGC 
TGTGAGGAGG AGGAGGATGA AGACTCGGGC AGTGAAGAGA GCCTGGTGGA CTCGGACTCC 
GACCCTGAGG AAAAGGAAGT CAACTTCCAG GCAGACCTGG CGGACCTGAC CTGTGAGATT 
GAGATCAAGC AGAAGCTGAT CGACGAGCTG GAGAACAGCC AGCGGCGGCT ACAGACGCTC 
AAGCACCAGT ACGAGGAGAA GCTCATTCTG CTGCAGAACA AGATCCGAGA CACACAGCTG 
GAGCGCGACC GCGTGCTGCA GAACCTCAGT ACTATGGAGT GCTACACGGA GGAGAAGGCC 
AACAAGATAA AGGCAGATTA TGAGAAGAGG CTTCGGGAGA TGAACCGGGA CCTGCAGAAG 
CTACAGGCTG CTCAAAAGGA GCATGCCAGG CTGCTCAAGA ACCAGTCTCG CTATGAGAGG 
GAGCTGAAGA AGCTGCAAAC GGAGGTAGCC GAAATGAAGA AGGCCAAGGT AGCCCTCATG 
AAGCAAATGC GTGAGGAACA GCAGCGGCGG CGACTGGTGG AGACCAAGAG GAACCGGGAG 
ATTGCTCAGC TCAAGAAGGA GCAACGGAGG CAGGAGTTCC AGATACGGGC CCTGGAATCT 
CAGAAGCGCC AGCAGGAAAT AGTTCTGAGG AGGAAAACCC AGGAGGTTTC CGCACTGAGG 
CGCTTAGCAA AGCCCATGTC TGAGAGAGTG GCCGGACGTG TAGGATTGAA GCCCCCCAAT 
ATGGATTCTG GGGCTGAGGT GTCTGCCAGC ACTACCTCAT CTGAGGCTGA ATCCGGAGCT 
CGCTCTGTCT CCAGCATAGT GCGTCAGTGG AACCGAAAAA TCAACCACTT CCTGGGGGAC 
CACCCGACAG CCACTGTCAA TGGCGCCCGC CCGGCCAGGA AAAAGTTCCA AAAGAAGGGA 
GCCAGCCAAA GCTTCAGTAA GGCCGCGAGA CTCAAGTGGC AGTCCCTGGA ACGGAGGATC 
ATTGACATTG TCATGCAGAG AATGACCATC GTCAACCTGG AGGCTGATAT GGAGCGTCTC 
ATAAAGAAAA GGGAGGAGCT CTTCCTCCTA CAGGAGGCAC TGCGGAGGAA GCGGGAGCGC 
CTGCAAGCTG AGAGCCCGGA GGAGGAGAAG GGACTACAGG AACTGGCTGA GGAGATCGAA 
GTGTTGGCAG CCAATATTGA CTACATCAAT GACAGCATCA CTGACTGCCA AGCCACTATC 
ATGCAGCTGG AGGAAACCAA GGAGGAGCTG GATTCGACAG ACACATCGGT GGTCATTAGT 
TCCTGCTCTC TGGCTGAAGC CCGCCTACTG CTAGACAACT TCCTCAAGGC ATCCATTGAC 
AAGGGACTTC AAGTAGCACA GAAGGAGGCC CAGATCCGGC TGCTGGAAGG ACGCCTCAGA 
CAAACAGACA TGACAGGCTC TTCCCAGAAC CACCTGCTCC TAGATGCTTT GCGTGAGAAG 
GCTGAGGCAC ACCCCGAGCT GCAGGCCCTC ATCTACAATG TGCAACATGA GAATGGCTAT 
GCCAGCACAG ATGAGGAAGT TTCAGAGTTC TCTGAGGGGA GCTTTTCCCA GTCATTCACC 
ATGAAAGGCT CCACCAGCCA TGATGACTTC AAGTTCAAGG GTGAGCCCAA GCTGTCCGCA 
CAAATGAAGG CCGTGTCTGC GGAGTGCCTA GGACCCCCAC TGGACAGCTC CACCAAGAAC 
ATCACCAAGT CCCTGGCCTC CCTCGTTGAG ATCAAAGAGG ATGGGGTGGG CTTCTCTATC 
CGGGACCCAT ACTACCGAGA CAAGGTCTCA CGCACCGTCA GCCTGCCCAC CCGGGGCAGC 
ACTTTCCCTC GGCAGTCTCG AGGTGCCACG GATACATCCC CTCTGACCCG CAGGAAGTCT 
TATGACCGGG GACAGCCCAT CAGATCAACA GACATAGGAT TCACACCTCC CTCATCACCT 
CCCACTCGGC CCCGCAACGA CCGCAATGTC TTTTCTCGCC TCACCAGCAA TCAGAGCCAG 
GGCTCGGCAC TGGACAAGTC TGATGACAGC GACTCCTCTT TGTCGGAGGT CCTGAGAGGC 
ATCATCACCC CCATTGGCGG AGCAAAGGGA GCGCGGACGG CCCCGTTGCA GTGTGTCTCC 
ATGGCCGAGG GCCACACCAA GCCCATCCTC TGCCTGGATG CCACCGATGA ATTACTTTTC 
ACTGGATCCA AAGACCGTAG CTGCAAGATG TGGAACCTGG TAACAGGGCA GGAGATTGCA 
GCTCTCAAGG GCCACCCCAA CAACGTGGTT TCTATCAAAT ACTGCAGCCA CTCAGGGCTG 
GTGTTCTCTG TGTCCAGCTC CTACGTCAAG GTGTGGGACA TCCGGGACTC AGCCAAGTGC 
ATCCGGACGC TCACGTCCTC AGGCCAGGTG ATCTCAGGAG ATGCTTGCAT GGCCACATCC 
ACCCGTGCCA TCACCAGTTC CCAGGGAGAG CATCAGATTA ACCAGATGGC CCTCAGCCCC 
TCGGGCACCA TGTTATATGT TGCCTCCGGC AACGCTGTCC GCATCTGGGA GCTCAACAGG 
TTCCAGCCCA TTGGCAAACT GACCGGCCAC ATTGGCCCAG TGATGTGCCT GACAGTCACC 
CAGACTTCAA ACCAGCATGA CCTCGTGGTG ACTGGCTCCA AGGACCACTA TGTGAAGATG 
TTCCAGCTGG GCGACTGTGT AACGGGCACC ATTGGCCCAA CCCACAACTT TGAGCCCCCT 
CACTATGACG GTATCGAATG CCTGGCCATC CAAGGAGATA TACTGTTCAG TGGTTCCAGA 
GACAATGGCA TCAAGAAGTG GGACCTGGAT CAGCAGGAGC TCATTCAGCA AATCCCCAAC 
GCTCACAAGG ACTGGGTGTG CGCCCTGGCC TTTGTACCCG GCCGGCCCAT GTTACTGAGC 
GCCTGCCGGG CAGGCTTCAT CAAGGTCTGG AATGTGGACA ACTTCACGCC CATTGGTGAG 
ATCAAGGGCC ATGACAGCCC CATCAATGCC ATCTGCACCA ACAGCAAGCA CATCTTCACG 
GCCTCCAGTG ACCTGACAGT GAAGTTCTGG AGCATGCGGC GGCTACCAGC AGCCCACCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21b ( XM_006249857.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249919.1 Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X5, mRNA.

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CloneID	ORa35360
Clone ID Related Accession (Same CDS sequence)	XM_006249857.3
Accession Version	XM_006249857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAGTCTGA TGACAGCGAC TCCTCTTTGT CGGAGGTCCT GAGAGGCATC 
ATCACCCCCA TTGGCGGAGC AAAGGGAGCG CGGACGGCCC CGTTGCAGTG TGTCTCCATG 
GCCGAGGGCC ACACCAAGCC CATCCTCTGC CTGGATGCCA CCGATGAATT ACTTTTCACT 
GGATCCAAAG ACCGTAGCTG CAAGATGTGG AACCTGGTAA CAGGGCAGGA GATTGCAGCT 
CTCAAGGGCC ACCCCAACAA CGTGGTTTCT ATCAAATACT GCAGCCACTC AGGGCTGGTG 
TTCTCTGTGT CCAGCTCCTA CGTCAAGGTG TGGGACATCC GGGACTCAGC CAAGTGCATC 
CGGACGCTCA CGTCCTCAGG CCAGGTGATC TCAGGAGATG CTTGCATGGC CACATCCACC 
CGTGCCATCA CCAGTTCCCA GGGAGAGCAT CAGATTAACC AGATGGCCCT CAGCCCCTCG 
GGCACCATGT TATATGTTGC CTCCGGCAAC GCTGTCCGCA TCTGGGAGCT CAACAGGTTC 
CAGCCCATTG GCAAACTGAC CGGCCACATT GGCCCAGTGA TGTGCCTGAC AGTCACCCAG 
ACTTCAAACC AGCATGACCT CGTGGTGACT GGCTCCAAGG ACCACTATGT GAAGATGTTC 
CAGCTGGGCG ACTGTGTAAC GGGCACCATT GGCCCAACCC ACAACTTTGA GCCCCCTCAC 
TATGACGGTA TCGAATGCCT GGCCATCCAA GGAGATATAC TGTTCAGTGG TTCCAGAGAC 
AATGGCATCA AGAAGTGGGA CCTGGATCAG CAGGAGCTCA TTCAGCAAAT CCCCAACGCT 
CACAAGGACT GGGTGTGCGC CCTGGCCTTT GTACCCGGCC GGCCCATGTT ACTGAGCGCC 
TGCCGGGCAG GCTTCATCAA GGTCTGGAAT GTGGACAACT TCACGCCCAT TGGTGAGATC 
AAGGGCCATG ACAGCCCCAT CAATGCCATC TGCACCAACA GCAAGCACAT CTTCACGGCC 
TCCAGTGACC TGACAGTGAA GTTCTGGAGC ATGCGGCGGC TACCAGCAGC CCACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249919.1
CDS	319..5178
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKSDDSDSSLSEVLRGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDLTVKFWSMRRLPAAHP

Target ORF information:

RefSeq Version	XM_006249857.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249857.3

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAGTCTGA TGACAGCGAC TCCTCTTTGT CGGAGGTCCT GAGAGGCATC 
ATCACCCCCA TTGGCGGAGC AAAGGGAGCG CGGACGGCCC CGTTGCAGTG TGTCTCCATG 
GCCGAGGGCC ACACCAAGCC CATCCTCTGC CTGGATGCCA CCGATGAATT ACTTTTCACT 
GGATCCAAAG ACCGTAGCTG CAAGATGTGG AACCTGGTAA CAGGGCAGGA GATTGCAGCT 
CTCAAGGGCC ACCCCAACAA CGTGGTTTCT ATCAAATACT GCAGCCACTC AGGGCTGGTG 
TTCTCTGTGT CCAGCTCCTA CGTCAAGGTG TGGGACATCC GGGACTCAGC CAAGTGCATC 
CGGACGCTCA CGTCCTCAGG CCAGGTGATC TCAGGAGATG CTTGCATGGC CACATCCACC 
CGTGCCATCA CCAGTTCCCA GGGAGAGCAT CAGATTAACC AGATGGCCCT CAGCCCCTCG 
GGCACCATGT TATATGTTGC CTCCGGCAAC GCTGTCCGCA TCTGGGAGCT CAACAGGTTC 
CAGCCCATTG GCAAACTGAC CGGCCACATT GGCCCAGTGA TGTGCCTGAC AGTCACCCAG 
ACTTCAAACC AGCATGACCT CGTGGTGACT GGCTCCAAGG ACCACTATGT GAAGATGTTC 
CAGCTGGGCG ACTGTGTAAC GGGCACCATT GGCCCAACCC ACAACTTTGA GCCCCCTCAC 
TATGACGGTA TCGAATGCCT GGCCATCCAA GGAGATATAC TGTTCAGTGG TTCCAGAGAC 
AATGGCATCA AGAAGTGGGA CCTGGATCAG CAGGAGCTCA TTCAGCAAAT CCCCAACGCT 
CACAAGGACT GGGTGTGCGC CCTGGCCTTT GTACCCGGCC GGCCCATGTT ACTGAGCGCC 
TGCCGGGCAG GCTTCATCAA GGTCTGGAAT GTGGACAACT TCACGCCCAT TGGTGAGATC 
AAGGGCCATG ACAGCCCCAT CAATGCCATC TGCACCAACA GCAAGCACAT CTTCACGGCC 
TCCAGTGACC TGACAGTGAA GTTCTGGAGC ATGCGGCGGC TACCAGCAGC CCACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kif21b ( XM_006249858.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249920.1 Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X6, mRNA.

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CloneID	ORa35361
Clone ID Related Accession (Same CDS sequence)	XM_006249858.3
Accession Version	XM_006249858.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kinesin-like protein KIF21B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAAGGCAT CATCACCCCC ATTGGCGGAG CAAAGGGAGC GCGGACGGCC 
CCGTTGCAGT GTGTCTCCAT GGCCGAGGGC CACACCAAGC CCATCCTCTG CCTGGATGCC 
ACCGATGAAT TACTTTTCAC TGGATCCAAA GACCGTAGCT GCAAGATGTG GAACCTGGTA 
ACAGGGCAGG AGATTGCAGC TCTCAAGGGC CACCCCAACA ACGTGGTTTC TATCAAATAC 
TGCAGCCACT CAGGGCTGGT GTTCTCTGTG TCCAGCTCCT ACGTCAAGGT GTGGGACATC 
CGGGACTCAG CCAAGTGCAT CCGGACGCTC ACGTCCTCAG GCCAGGTGAT CTCAGGAGAT 
GCTTGCATGG CCACATCCAC CCGTGCCATC ACCAGTTCCC AGGGAGAGCA TCAGATTAAC 
CAGATGGCCC TCAGCCCCTC GGGCACCATG TTATATGTTG CCTCCGGCAA CGCTGTCCGC 
ATCTGGGAGC TCAACAGGTT CCAGCCCATT GGCAAACTGA CCGGCCACAT TGGCCCAGTG 
ATGTGCCTGA CAGTCACCCA GACTTCAAAC CAGCATGACC TCGTGGTGAC TGGCTCCAAG 
GACCACTATG TGAAGATGTT CCAGCTGGGC GACTGTGTAA CGGGCACCAT TGGCCCAACC 
CACAACTTTG AGCCCCCTCA CTATGACGGT ATCGAATGCC TGGCCATCCA AGGAGATATA 
CTGTTCAGTG GTTCCAGAGA CAATGGCATC AAGAAGTGGG ACCTGGATCA GCAGGAGCTC 
ATTCAGCAAA TCCCCAACGC TCACAAGGAC TGGGTGTGCG CCCTGGCCTT TGTACCCGGC 
CGGCCCATGT TACTGAGCGC CTGCCGGGCA GGCTTCATCA AGGTCTGGAA TGTGGACAAC 
TTCACGCCCA TTGGTGAGAT CAAGGGCCAT GACAGCCCCA TCAATGCCAT CTGCACCAAC 
AGCAAGCACA TCTTCACGGC CTCCAGTGAC CTGACAGTGA AGTTCTGGAG CATGCGGCGG 
CTACCAGCAG CCCACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249920.1
CDS	319..5139
Translation	MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQEQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDTVTSEEEQGIIPRAIAHLFRGIDERKRRAQEQGVTGPEFKVSAQFLELYNEEILDLFDSTRDPDARHRRSNIKIHEDANGGIYTTGVTSRLINSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRVCAQPDLVNETVTGLPDGTAPTGTEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARNPYSLGASPAGPAFGGSPASSMEDASEVIRRAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRLQTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHARLLKNQSRYERELKKLQTEVAEMKKAKVALMKQMREEQQRRRLVETKRNREIAQLKKEQRRQEFQIRALESQKRQQEIVLRRKTQEVSALRRLAKPMSERVAGRVGLKPPNMDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPTATVNGARPARKKFQKKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRKRERLQAESPEEEKGLQELAEEIEVLAANIDYINDSITDCQATIMQLEETKEELDSTDTSVVISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMTGSSQNHLLLDALREKAEAHPELQALIYNVQHENGYASTDEEVSEFSEGSFSQSFTMKGSTSHDDFKFKGEPKLSAQMKAVSAECLGPPLDSSTKNITKSLASLVEIKEDGVGFSIRDPYYRDKVSRTVSLPTRGSTFPRQSRGATDTSPLTRRKSYDRGQPIRSTDIGFTPPSSPPTRPRNDRNVFSRLTSNQSQGSALDKGIITPIGGAKGARTAPLQCVSMAEGHTKPILCLDATDELLFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSSSYVKVWDIRDSAKCIRTLTSSGQVISGDACMATSTRAITSSQGEHQINQMALSPSGTMLYVASGNAVRIWELNRFQPIGKLTGHIGPVMCLTVTQTSNQHDLVVTGSKDHYVKMFQLGDCVTGTIGPTHNFEPPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFVPGRPMLLSACRAGFIKVWNVDNFTPIGEIKGHDSPINAICTNSKHIFTASSDLTVKFWSMRRLPAAHP

Target ORF information:

RefSeq Version	XM_006249858.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus kinesin family member 21B (Kif21b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249858.3

ORF Insert Sequence:

ATGGCTGGTC AGGGCGACTG CTGCGTCAAA GTGGCCGTCA GGATCCGGCC CCAGCTATCA 
AAGGAGAAGA TTGAAGGCTG TCACATCTGT ACCTCCGTTA CCCCTGGGGA GCCCCAGGTC 
CTGCTGGGGA AGGACAAGGC CTTCACCTAT GACTTTGTCT TTGACCTGGA CACCTGGCAA 
GAACAAATCT ATTCGACCTG CGTGAGCAAA CTCATCGAAG GCTGCTTTGA GGGCTATAAC 
GCCACAGTAC TGGCCTATGG ACAGACAGGG GCTGGGAAGA CATACACTAT GGGCACCGGC 
TTTGACACAG TGACGTCAGA AGAGGAGCAG GGCATCATCC CAAGGGCCAT TGCGCATCTC 
TTCAGGGGCA TCGACGAACG CAAGCGTCGG GCGCAGGAGC AGGGTGTGAC TGGGCCTGAG 
TTCAAAGTCA GTGCCCAATT CCTGGAGCTC TACAACGAAG AGATCCTTGA CCTGTTTGAC 
AGCACCCGCG ACCCTGATGC CCGCCACCGC AGGTCCAACA TCAAGATCCA CGAGGATGCC 
AATGGCGGCA TCTACACCAC AGGGGTCACT TCTCGCCTCA TCAACTCCCA GGAGGAGCTG 
ATCCAGTGCC TGAAGCAGGG CGCCCTGTCA CGAACCACGG CCAGCACCCA GATGAACGTT 
CAAAGTTCCC GATCCCACGC CATCTTCACC ATTCACCTGT GCCAGATGCG CGTGTGTGCC 
CAGCCTGACC TGGTGAATGA GACAGTGACT GGGCTTCCGG ATGGAACGGC CCCCACGGGC 
ACTGAGTATG AGACGCTCAC TGCTAAGTTC CACTTTGTGG ACTTGGCGGG CTCAGAGCGG 
CTGAAACGGA CAGGGGCCAC CGGTGAACGA GCCAAAGAGG GCATCTCCAT CAACTGTGGT 
CTGCTGGCCT TGGGCAATGT GATCAGTGCC TTGGGAGATC AGAGCAAGAA AGTCGTGCAC 
GTGCCCTACA GGGACTCCAA GCTCACTCGG CTGCTCCAGG ACTCTCTTGG AGGCAACAGC 
CAGACCATCA TGATCGCCTG TGTGAGCCCC TCAGATCGGG ACTTCATGGA GACGCTCAAC 
ACTCTCAAAT ATGCCAATCG GGCTCGAAAC ATCAAAAACA AAGTGGTAGT GAACCAGGAC 
AAGACCAGCC AGCAGATCAG CGCGCTGCGA GCCGAGATCG CACGCCTGCA GATGGAGTTG 
ATGGAATACA AGGCGGGCAA GCGAGTGATT GGGGAGGACG GTGCAGAGGG CTACAGCGAC 
CTGTTCCGGG AGAACGCCAT GCTACAGAAG GAGAACGGAG CACTGAGACT CCGAGTGAAG 
GCCATGCAGG AAGCCATTGA TGCCATCAAC AACCGGGTCA CACAACTCAT GAGTCAGGAG 
GCTAACCTGC TTCTGGCCAA GGCTGGGGAT GGCAACGAGG CCATTGGCGC TCTGATCCAG 
AACTACATCC GGGAGATTGA GGAGCTTCGC ACGAAGCTTC TGGAGAGTGA GGCTATGAAT 
GAGTCCCTCC GAAGGAGCCT CTCCCGGGCT TCCGCTCGGA ACCCCTACTC CCTGGGAGCA 
TCTCCAGCAG GTCCAGCCTT TGGGGGCAGC CCGGCCAGCT CCATGGAAGA TGCTTCTGAA 
GTGATCCGCA GGGCCAAGCA AGACCTGGAG CGGCTGAAAA AGAAAGAGGT CAGGCAGCGG 
AGGAAGAGCC CGGAGAAAGA AGCCTTCAAA AAGAGGGCAA AACTCCAGCA GGAAAACAGT 
GAGGAGACGG ATGAGAACGA GGCTGAGGAG GAGGAAGAAG AACGAGATGA GAGTGGCTGT 
GAGGAGGAGG AGGATGAAGA CTCGGGCAGT GAAGAGAGCC TGGTGGACTC GGACTCCGAC 
CCTGAGGAAA AGGAAGTCAA CTTCCAGGCA GACCTGGCGG ACCTGACCTG TGAGATTGAG 
ATCAAGCAGA AGCTGATCGA CGAGCTGGAG AACAGCCAGC GGCGGCTACA GACGCTCAAG 
CACCAGTACG AGGAGAAGCT CATTCTGCTG CAGAACAAGA TCCGAGACAC ACAGCTGGAG 
CGCGACCGCG TGCTGCAGAA CCTCAGTACT ATGGAGTGCT ACACGGAGGA GAAGGCCAAC 
AAGATAAAGG CAGATTATGA GAAGAGGCTT CGGGAGATGA ACCGGGACCT GCAGAAGCTA 
CAGGCTGCTC AAAAGGAGCA TGCCAGGCTG CTCAAGAACC AGTCTCGCTA TGAGAGGGAG 
CTGAAGAAGC TGCAAACGGA GGTAGCCGAA ATGAAGAAGG CCAAGGTAGC CCTCATGAAG 
CAAATGCGTG AGGAACAGCA GCGGCGGCGA CTGGTGGAGA CCAAGAGGAA CCGGGAGATT 
GCTCAGCTCA AGAAGGAGCA ACGGAGGCAG GAGTTCCAGA TACGGGCCCT GGAATCTCAG 
AAGCGCCAGC AGGAAATAGT TCTGAGGAGG AAAACCCAGG AGGTTTCCGC ACTGAGGCGC 
TTAGCAAAGC CCATGTCTGA GAGAGTGGCC GGACGTGTAG GATTGAAGCC CCCCAATATG 
GATTCTGGGG CTGAGGTGTC TGCCAGCACT ACCTCATCTG AGGCTGAATC CGGAGCTCGC 
TCTGTCTCCA GCATAGTGCG TCAGTGGAAC CGAAAAATCA ACCACTTCCT GGGGGACCAC 
CCGACAGCCA CTGTCAATGG CGCCCGCCCG GCCAGGAAAA AGTTCCAAAA GAAGGGAGCC 
AGCCAAAGCT TCAGTAAGGC CGCGAGACTC AAGTGGCAGT CCCTGGAACG GAGGATCATT 
GACATTGTCA TGCAGAGAAT GACCATCGTC AACCTGGAGG CTGATATGGA GCGTCTCATA 
AAGAAAAGGG AGGAGCTCTT CCTCCTACAG GAGGCACTGC GGAGGAAGCG GGAGCGCCTG 
CAAGCTGAGA GCCCGGAGGA GGAGAAGGGA CTACAGGAAC TGGCTGAGGA GATCGAAGTG 
TTGGCAGCCA ATATTGACTA CATCAATGAC AGCATCACTG ACTGCCAAGC CACTATCATG 
CAGCTGGAGG AAACCAAGGA GGAGCTGGAT TCGACAGACA CATCGGTGGT CATTAGTTCC 
TGCTCTCTGG CTGAAGCCCG CCTACTGCTA GACAACTTCC TCAAGGCATC CATTGACAAG 
GGACTTCAAG TAGCACAGAA GGAGGCCCAG ATCCGGCTGC TGGAAGGACG CCTCAGACAA 
ACAGACATGA CAGGCTCTTC CCAGAACCAC CTGCTCCTAG ATGCTTTGCG TGAGAAGGCT 
GAGGCACACC CCGAGCTGCA GGCCCTCATC TACAATGTGC AACATGAGAA TGGCTATGCC 
AGCACAGATG AGGAAGTTTC AGAGTTCTCT GAGGGGAGCT TTTCCCAGTC ATTCACCATG 
AAAGGCTCCA CCAGCCATGA TGACTTCAAG TTCAAGGGTG AGCCCAAGCT GTCCGCACAA 
ATGAAGGCCG TGTCTGCGGA GTGCCTAGGA CCCCCACTGG ACAGCTCCAC CAAGAACATC 
ACCAAGTCCC TGGCCTCCCT CGTTGAGATC AAAGAGGATG GGGTGGGCTT CTCTATCCGG 
GACCCATACT ACCGAGACAA GGTCTCACGC ACCGTCAGCC TGCCCACCCG GGGCAGCACT 
TTCCCTCGGC AGTCTCGAGG TGCCACGGAT ACATCCCCTC TGACCCGCAG GAAGTCTTAT 
GACCGGGGAC AGCCCATCAG ATCAACAGAC ATAGGATTCA CACCTCCCTC ATCACCTCCC 
ACTCGGCCCC GCAACGACCG CAATGTCTTT TCTCGCCTCA CCAGCAATCA GAGCCAGGGC 
TCGGCACTGG ACAAAGGCAT CATCACCCCC ATTGGCGGAG CAAAGGGAGC GCGGACGGCC 
CCGTTGCAGT GTGTCTCCAT GGCCGAGGGC CACACCAAGC CCATCCTCTG CCTGGATGCC 
ACCGATGAAT TACTTTTCAC TGGATCCAAA GACCGTAGCT GCAAGATGTG GAACCTGGTA 
ACAGGGCAGG AGATTGCAGC TCTCAAGGGC CACCCCAACA ACGTGGTTTC TATCAAATAC 
TGCAGCCACT CAGGGCTGGT GTTCTCTGTG TCCAGCTCCT ACGTCAAGGT GTGGGACATC 
CGGGACTCAG CCAAGTGCAT CCGGACGCTC ACGTCCTCAG GCCAGGTGAT CTCAGGAGAT 
GCTTGCATGG CCACATCCAC CCGTGCCATC ACCAGTTCCC AGGGAGAGCA TCAGATTAAC 
CAGATGGCCC TCAGCCCCTC GGGCACCATG TTATATGTTG CCTCCGGCAA CGCTGTCCGC 
ATCTGGGAGC TCAACAGGTT CCAGCCCATT GGCAAACTGA CCGGCCACAT TGGCCCAGTG 
ATGTGCCTGA CAGTCACCCA GACTTCAAAC CAGCATGACC TCGTGGTGAC TGGCTCCAAG 
GACCACTATG TGAAGATGTT CCAGCTGGGC GACTGTGTAA CGGGCACCAT TGGCCCAACC 
CACAACTTTG AGCCCCCTCA CTATGACGGT ATCGAATGCC TGGCCATCCA AGGAGATATA 
CTGTTCAGTG GTTCCAGAGA CAATGGCATC AAGAAGTGGG ACCTGGATCA GCAGGAGCTC 
ATTCAGCAAA TCCCCAACGC TCACAAGGAC TGGGTGTGCG CCCTGGCCTT TGTACCCGGC 
CGGCCCATGT TACTGAGCGC CTGCCGGGCA GGCTTCATCA AGGTCTGGAA TGTGGACAAC 
TTCACGCCCA TTGGTGAGAT CAAGGGCCAT GACAGCCCCA TCAATGCCAT CTGCACCAAC 
AGCAAGCACA TCTTCACGGC CTCCAGTGAC CTGACAGTGA AGTTCTGGAG CATGCGGCGG 
CTACCAGCAG CCCACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Activity-Dependent Regulation of Distinct Transport and Cytoskeletal Remodeling Functions of the Dendritic Kinesin KIF21B.
Neuron92(4)857-872(2016 Nov)
Ghiretti AE,Thies E,Tokito MK,Lin T,Ostap EM,Kneussel M,Holzbaur ELF

Three novel mutations in KIF21A highlight the importance of the third coiled-coil stalk domain in the etiology of CFEOM1.
BMC genetics826(2007 May)
Chan WM,Andrews C,Dragan L,Fredrick D,Armstrong L,Lyons C,Geraghty MT,Hunter DG,Yazdani A,Traboulsi EI,Pott JW,Gutowski NJ,Ellard S,Young E,Hanisch F,Koc F,Schnall B,Engle EC

Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1).
Nature genetics35(4)318-21(2003 Dec)
Yamada K,Andrews C,Chan WM,McKeown CA,Magli A,de Berardinis T,Loewenstein A,Lazar M,O'Keefe M,Letson R,London A,Ruttum M,Matsumoto N,Saito N,Morris L,Del Monte M,Johnson RH,Uyama E,Houtman WA,de Vries B,Carlow TJ,Hart BL,Krawiecki N,Shoffner J,Vogel MC,Katowitz J,Goldstein SM,Levin AV,Sener EC,Ozturk BT,Akarsu AN,Brodsky MC,Hanisch F,Cruse RP,Zubcov AA,Robb RM,Roggenk?emper P,Gottlob I,Kowal L,Battu R,Traboulsi EI,Franceschini P,Newlin A,Demer JL,Engle EC

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Kif21b cDNA ORF clone, Rattus norvegicus(Norway rat)

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