Hcls1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcls1
Entrez Gene ID	288077
Full Name	hematopoietic cell specific Lyn substrate 1
General protein information	Preferred Names hematopoietic cell specific Lyn substrate 1 Names hematopoietic lineage cell-specific protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011898.1	NP_001011898.1	hematopoietic lineage cell-specific protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCLS1	NP_005326.2
Mus musculus (house mouse)	Hcls1	NP_032251.2
Rattus norvegicus (Norway rat)	Hcls1	NP_001011898.1
Bos taurus (cattle)	HCLS1	NP_001030229.1
Gallus gallus (chicken)	HCLS1	NP_001026572.1
Pan troglodytes (chimpanzee)	HCLS1	XP_516684.2
	HCLS1	XP_001111514.1
Canis lupus familiaris (dog)	HCLS1	XP_005639607.1

Gene Ontology
Bibliography

Related articles in PubMed

Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis.
Skokowa J, Klimiankou M, Klimenkova O, Lan D, Gupta K, Hussein K, Carrizosa E, Kusnetsova I, Li Z, Sustmann C, Ganser A, Zeidler C, Kreipe HH, Burkhardt J, Grosschedl R, Welte K
Nature medicine18(10)1550-9(2012 Oct)

Liar, a novel Lyn-binding nuclear/cytoplasmic shuttling protein that influences erythropoietin-induced differentiation.
Samuels AL, Klinken SP, Ingley E
Blood113(16)3845-56(2009 Apr)

HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells.
Ingley E, Sarna MK, Beaumont JG, Tilbrook PA, Tsai S, Takemoto Y, Williams JH, Klinken SP
The Journal of biological chemistry275(11)7887-93(2000 Mar)

Examining the specificity of Src homology 3 domain--ligand interactions with alkaline phosphatase fusion proteins.
Yamabhai M, Kay BK
Analytical biochemistry247(1)143-51(1997 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hcls1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcls1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01463	NM_001011898.1 Latest version!	Rattus norvegicus hematopoietic cell specific Lyn substrate 1 (Hcls1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$279.30 $399.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcls1 ( NM_001011898.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001011898.1 Rattus norvegicus hematopoietic cell specific Lyn substrate 1 (Hcls1), mRNA.

Price & Availability↑

CloneID	ORa01463
Clone ID Related Accession (Same CDS sequence)	NM_001011898.1
Accession Version	NM_001011898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	hematopoietic lineage cell-specific protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079081.1. On Feb 11, 2005 this sequence version replaced XM_221421.2. ##Evidence-Data-START## Transcript exon combination :: BC079081.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTGGAAGT CTGTAGTGGG GCACAATGTA TCGGTTTCCG TGGAGACCCA GGGTGATGAC 
TGGGACACAG ACCCTGACTT TGTGAATGAC ATCTCTGAGA AGGAACAACG GTGGGGAGCC 
AAGACCATTG AGGGCTCTGG ACGCACAGAG CATATCAACA TCCACCAGCT GAGGAACAAA 
GTGTCCGAGG AGCATGACAT TCTCAAGAAG AGAGAGCTGG AGTCGGGGCC TAAAGCCTCC 
CATGGCTATG GGGGTCGGTT TGGAGTGGAA AGAGACCGGA TGGACAAGAG TGCCGTGGGC 
CACGAGTATG TTGCTGAAGT GGAGAAACAC TCGTCTCAGA CTGATGCCGC CAAAGGCTTT 
GGGGGCAAAT ATGGAGTTGA GAGGGACCGG GCAGACAAGT CAGCGGTGGG CTTTGACTAC 
AAAGGAGAGG TGGAGAAGCA CGCATCTCAG AAAGATTACT CTCATGGCTT TGGTGGCCGC 
TACGGGGTAG AGAAGGATAA ACGGGACAAA GCAGCTCTGG GATATGACTA CAAGGGAGAG 
ACGGAGAAGC ACGAGTCTCA GCGAGATTAT GCCAAGGGCT TTGGTGGTCA ATATGGAATC 
CAGAAAGACA GAGTGGATAA GAGTGCTGTT GGCTTCAATG AAATGGAGGC CCCAACCACG 
GCATATAAGA AGACAACACC CATAGAAGCT GCTTCCAGTG GTGCCCGTGG GCTGAAGGCA 
AAATTCGAGT CCATGGCTGA GGAGAAGAGG AAGCGAGAAG AAGAAGAGAA GGCACAACAG 
ATGGCCAGGC AGCAGCAAGA ACGAAAGGCT ATGGTGAAGA TGAGCCGTGA AGCTCAGCAG 
CCATCCGTGC CTGTGGACGA GCCCGCAGCA CCAGCCCCGT TGCCCAAGAA GATCTCCTCA 
GAGGTCTGGC CTCCAGCAGA GAGTCACCTA CCACCACAGT CCGAGCCCGT GGGAGGCAGA 
AGAGAATACC CTGTGCCCTC CCTGCCCACG AGACAGCCTC CACCTCAGAA TCCCCTCGAG 
GACAATGAGG AGCCCCCAGC TCTGCCCCCT AGGACTCCAG AAGGCCTCCA GGTGGTAGAA 
GAGCCAGTGT ACGAAGCAGC ACCCGAGCCC GAGCCTGAGC CCGAGCCTGA CTATGAGGAT 
GTTGGAGAGT TAGATCGGCC GGATGAGGAA GCAGAGGGAG ACTATGAGGA TGTGCTGGAG 
CCCGAGGACA CCCCTTCTCT ATCCTACCAG GCTGGACACT CAGCTGGGGC TGGCGGTGCA 
GGGATCTCTG CTGTAGCCCT TTATGATTAC CAAGGAGAGG GAAGTGATGA GCTGTCCTTT 
GACCCAGATG ATGTCATCAC TGACATTGAG ATGGTTGATG AAGGCTGGTG GCGGGGCCGA 
TGTCATGGCC ACTTTGGACT CTTTCCTGCA AACTATGTCA AGCTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011898.1
CDS	32..1462
Translation	MWKSVVGHNVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKKRELESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDYKGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESMAEEKRKREEEEKAQQMARQQQERKAMVKMSREAQQPSVPVDEPAAPAPLPKKISSEVWPPAESHLPPQSEPVGGRREYPVPSLPTRQPPPQNPLEDNEEPPALPPRTPEGLQVVEEPVYEAAPEPEPEPEPDYEDVGELDRPDEEAEGDYEDVLEPEDTPSLSYQAGHSAGAGGAGISAVALYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLL

Target ORF information:

RefSeq Version	NM_001011898.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hematopoietic cell specific Lyn substrate 1 (Hcls1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011898.1

ORF Insert Sequence:

ATGTGGAAGT CTGTAGTGGG GCACAATGTA TCGGTTTCCG TGGAGACCCA GGGTGATGAC 
TGGGACACAG ACCCTGACTT TGTGAATGAC ATCTCTGAGA AGGAACAACG GTGGGGAGCC 
AAGACCATTG AGGGCTCTGG ACGCACAGAG CATATCAACA TCCACCAGCT GAGGAACAAA 
GTGTCCGAGG AGCATGACAT TCTCAAGAAG AGAGAGCTGG AGTCGGGGCC TAAAGCCTCC 
CATGGCTATG GGGGTCGGTT TGGAGTGGAA AGAGACCGGA TGGACAAGAG TGCCGTGGGC 
CACGAGTATG TTGCTGAAGT GGAGAAACAC TCGTCTCAGA CTGATGCCGC CAAAGGCTTT 
GGGGGCAAAT ATGGAGTTGA GAGGGACCGG GCAGACAAGT CAGCGGTGGG CTTTGACTAC 
AAAGGAGAGG TGGAGAAGCA CGCATCTCAG AAAGATTACT CTCATGGCTT TGGTGGCCGC 
TACGGGGTAG AGAAGGATAA ACGGGACAAA GCAGCTCTGG GATATGACTA CAAGGGAGAG 
ACGGAGAAGC ACGAGTCTCA GCGAGATTAT GCCAAGGGCT TTGGTGGTCA ATATGGAATC 
CAGAAAGACA GAGTGGATAA GAGTGCTGTT GGCTTCAATG AAATGGAGGC CCCAACCACG 
GCATATAAGA AGACAACACC CATAGAAGCT GCTTCCAGTG GTGCCCGTGG GCTGAAGGCA 
AAATTCGAGT CCATGGCTGA GGAGAAGAGG AAGCGAGAAG AAGAAGAGAA GGCACAACAG 
ATGGCCAGGC AGCAGCAAGA ACGAAAGGCT ATGGTGAAGA TGAGCCGTGA AGCTCAGCAG 
CCATCCGTGC CTGTGGACGA GCCCGCAGCA CCAGCCCCGT TGCCCAAGAA GATCTCCTCA 
GAGGTCTGGC CTCCAGCAGA GAGTCACCTA CCACCACAGT CCGAGCCCGT GGGAGGCAGA 
AGAGAATACC CTGTGCCCTC CCTGCCCACG AGACAGCCTC CACCTCAGAA TCCCCTCGAG 
GACAATGAGG AGCCCCCAGC TCTGCCCCCT AGGACTCCAG AAGGCCTCCA GGTGGTAGAA 
GAGCCAGTGT ACGAAGCAGC ACCCGAGCCC GAGCCTGAGC CCGAGCCTGA CTATGAGGAT 
GTTGGAGAGT TAGATCGGCC GGATGAGGAA GCAGAGGGAG ACTATGAGGA TGTGCTGGAG 
CCCGAGGACA CCCCTTCTCT ATCCTACCAG GCTGGACACT CAGCTGGGGC TGGCGGTGCA 
GGGATCTCTG CTGTAGCCCT TTATGATTAC CAAGGAGAGG GAAGTGATGA GCTGTCCTTT 
GACCCAGATG ATGTCATCAC TGACATTGAG ATGGTTGATG AAGGCTGGTG GCGGGGCCGA 
TGTCATGGCC ACTTTGGACT CTTTCCTGCA AACTATGTCA AGCTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interactions among HCLS1, HAX1 and LEF-1 proteins are essential for G-CSF-triggered granulopoiesis.
Nature medicine18(10)1550-9(2012 Oct)
Skokowa J,Klimiankou M,Klimenkova O,Lan D,Gupta K,Hussein K,Carrizosa E,Kusnetsova I,Li Z,Sustmann C,Ganser A,Zeidler C,Kreipe HH,Burkhardt J,Grosschedl R,Welte K

Liar, a novel Lyn-binding nuclear/cytoplasmic shuttling protein that influences erythropoietin-induced differentiation.
Blood113(16)3845-56(2009 Apr)
Samuels AL,Klinken SP,Ingley E

HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells.
The Journal of biological chemistry275(11)7887-93(2000 Mar)
Ingley E,Sarna MK,Beaumont JG,Tilbrook PA,Tsai S,Takemoto Y,Williams JH,Klinken SP

Examining the specificity of Src homology 3 domain--ligand interactions with alkaline phosphatase fusion proteins.
Analytical biochemistry247(1)143-51(1997 Apr)
Yamabhai M,Kay BK