Skap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Skap1
Entrez Gene ID	286975
Full Name	src kinase associated phosphoprotein 1
Synonyms	Scap1,Skap55
General protein information	Preferred Names src kinase associated phosphoprotein 1 Names src kinase-associated phosphoprotein 1 SKAP-55 SKAP55 adapter protein SKAP55 adaptor protein pp55 src family-associated phosphoprotein 1 src kinase-associated phosphoprotein of 55 kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26-q31
Summary	cytoplasmic phosphoprotein; member of the src family of kinase enzymes; may associate with mast cell immunoreceptor signal transducer (MIST) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597053.1	XP_017452542.1	src kinase-associated phosphoprotein 1 isoform X1
NM_173311.2	NP_775433.2	src kinase-associated phosphoprotein 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	skap1	NP_001073480.1
Xenopus tropicalis (tropical clawed frog)	skap1	NP_001004990.1
Mus musculus (house mouse)	Skap1	NP_001028358.1
Canis lupus familiaris (dog)	SKAP1	XP_537665.3
Rattus norvegicus (Norway rat)	Skap1	NP_775433.2
Homo sapiens (human)	SKAP1	NP_003717.3
Pan troglodytes (chimpanzee)	SKAP1	XP_523821.2
Bos taurus (cattle)	SKAP1	XP_002695990.2

Gene Ontology
Bibliography

Related articles in PubMed

Signaling Scaffold Protein IQGAP1 Interacts with Microtubule Plus-end Tracking Protein SKAP and Links Dynamic Microtubule Plus-end to Steer Cell Migration.
Cao D, Su Z, Wang W, Wu H, Liu X, Akram S, Qin B, Zhou J, Zhuang X, Adams G, Jin C, Wang X, Liu L, Hill DL, Wang D, Ding X, Yao X
The Journal of biological chemistry290(39)23766-80(2015 Sep)

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

RIAM links the ADAP/SKAP-55 signaling module to Rap1, facilitating T-cell-receptor-mediated integrin activation.
M?nasch? G, Kliche S, Chen EJ, Stradal TE, Schraven B, Koretzky G
Molecular and cellular biology27(11)4070-81(2007 Jun)

Targeting of MIST to Src-family kinases via SKAP55-SLAP-130 adaptor complex in mast cells.
Fujii Y, Wakahara S, Nakao T, Hara T, Ohtake H, Komurasaki T, Kitamura K, Tatsuno A, Fujiwara N, Hozumi N, Ra C, Kitamura D, Goitsuka R
FEBS letters540(1-3)111-6(2003 Apr)

SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates.
Wang H, Moon EY, Azouz A, Wu X, Smith A, Schneider H, Hogg N, Rudd CE
Nature immunology4(4)366-74(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Skap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Skap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03670	NM_173311.2 Latest version!	Rattus norvegicus src kinase associated phosphoprotein 1 (Skap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa57204	XM_017597053.1 Latest version!	Rattus norvegicus src kinase associated phosphoprotein 1 (Skap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Skap1 ( NM_173311.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775433.2 Rattus norvegicus src kinase associated phosphoprotein 1 (Skap1), mRNA.

Price & Availability↑

CloneID	ORa03670
Clone ID Related Accession (Same CDS sequence)	NM_173311.2
Accession Version	NM_173311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	src kinase-associated phosphoprotein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097931.1. On Jun 12, 2005 this sequence version replaced NM_173311.1. ##Evidence-Data-START## Transcript exon combination :: BC097931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCCA TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATGC TGAAGACTTT 
CTGGCGGAAG GCTTGCAGAA TGAGAACCTT AGTCCTGGGG CACAGGATCA GAGAGACCAC 
ATTCTCCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GACGACCTTG GCCAAGACGG CTCTGATGAA AATCTGAGTG GGACACACGG CCCAGCCCTC 
GCATCAGATG CATCCTTTTG GTCAGATTAT CAGGAGGAGG GAATAGACGA CATCATCAGA 
GGGGCTCAAG AGCTGGATAA CGTCATCAAA CAAGGATACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGATGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGAA CCTTCCTCAT TAAGGGATAC 
AACGTCCGGA TGGCCCCTCA CCTGCGGAAA GACTCCAAGA AAGACTCCTG CTTTGAACTG 
ACCTCCCAGG ATAGGCGCAG CTATGAGTTC ACAGCTTTTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTGACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAGGAAGA GGAAGACAAA GAACAGGAAG AAATGTACAA TGACATTGAT 
GGCTTTGACT CCGCAAGGTC CGGCTCCCAG GGCAGAGCCA TGGCCTTGCC TGAGCCTCTG 
GACAAAGAGG AGGACGTTTA CGAAGTCCTA CCAGATGAAG ATGATCTAGA AGAGGATGCA 
TGTGGCGCTC ATCGGCGGCG AGTGGACTAT GCCGATTATT ACCAGGGCCT CTGGGATTGC 
CATGGTGACC AGCCAGATGA ACTGTCCTTC CAACGAGGTG ACCTCATCCG CATTCTGAGC 
AAGGAGTACA ACATGTACGG CTGGTGGGTA GGAGAACTGA ACAGCATCAT TGGGATTGTT 
CCAAAGGACT ACCTCACCAC CGCCTTTGAA ATGGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775433.2
CDS	99..1163
Translation	MQAIALPEEICWLLEDAEDFLAEGLQNENLSPGAQDQRDHILRGFQQIKSRYCWDFQPQGDDLGQDGSDENLSGTHGPALASDASFWSDYQEEGIDDIIRGAQELDNVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGYNVRMAPHLRKDSKKDSCFELTSQDRRSYEFTAFSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEDKEQEEMYNDIDGFDSARSGSQGRAMALPEPLDKEEDVYEVLPDEDDLEEDACGAHRRRVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSIIGIVPKDYLTTAFEMEGR

Target ORF information:

RefSeq Version	NM_173311.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus src kinase associated phosphoprotein 1 (Skap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173311.2

ORF Insert Sequence:

ATGCAGGCCA TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATGC TGAAGACTTT 
CTGGCGGAAG GCTTGCAGAA TGAGAACCTT AGTCCTGGGG CACAGGATCA GAGAGACCAC 
ATTCTCCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GACGACCTTG GCCAAGACGG CTCTGATGAA AATCTGAGTG GGACACACGG CCCAGCCCTC 
GCATCAGATG CATCCTTTTG GTCAGATTAT CAGGAGGAGG GAATAGACGA CATCATCAGA 
GGGGCTCAAG AGCTGGATAA CGTCATCAAA CAAGGATACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGATGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGAA CCTTCCTCAT TAAGGGATAC 
AACGTCCGGA TGGCCCCTCA CCTGCGGAAA GACTCCAAGA AAGACTCCTG CTTTGAACTG 
ACCTCCCAGG ATAGGCGCAG CTATGAGTTC ACAGCTTTTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTGACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAGGAAGA GGAAGACAAA GAACAGGAAG AAATGTACAA TGACATTGAT 
GGCTTTGACT CCGCAAGGTC CGGCTCCCAG GGCAGAGCCA TGGCCTTGCC TGAGCCTCTG 
GACAAAGAGG AGGACGTTTA CGAAGTCCTA CCAGATGAAG ATGATCTAGA AGAGGATGCA 
TGTGGCGCTC ATCGGCGGCG AGTGGACTAT GCCGATTATT ACCAGGGCCT CTGGGATTGC 
CATGGTGACC AGCCAGATGA ACTGTCCTTC CAACGAGGTG ACCTCATCCG CATTCTGAGC 
AAGGAGTACA ACATGTACGG CTGGTGGGTA GGAGAACTGA ACAGCATCAT TGGGATTGTT 
CCAAAGGACT ACCTCACCAC CGCCTTTGAA ATGGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( XM_017597053.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452542.1 Rattus norvegicus src kinase associated phosphoprotein 1 (Skap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57204
Clone ID Related Accession (Same CDS sequence)	XM_017597053.1
Accession Version	XM_017597053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	src kinase-associated phosphoprotein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCA TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATGC TGAAGACTTT 
CTGGCGGAAG GCTTGCAGAA TGAGAACCTT AGTCCTGGGG CACAGGATCA GAGAGACCAC 
ATTCTCCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GACGACCTTG GCCAAGACGG CTCTGATGAA AATCTGAGTG GGACACACGG CCCAGCCCTC 
GCATCAGATG CATCCTTTTG GTCAGATTAT CAGGAGGAGG GAATAGACGA CATCATCAGA 
GGGGCTCAAG AGCTGGATAA CGTCATCAAA CAAGGATACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGATGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGAA CCTTCCTCAT TAAGGGATAC 
AACGTCCGGA TGGCCCCTCA CCTGCGGAAA GACTCCAAGA AAGACTCCTG CTTTGAACTG 
ACCTCCCAGG ATAGGCGCAG CTATGAGTTC ACAGCTTTTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTGACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAGGAAGA GGAAGACAAA GAACAGGAAG AAATGTACAA TGACATTGAT 
GGCTTTGACT CCGCAAGGTC CGGCTCCCAG GGCAGAGCCA TGGCCTTGCC TGAGCCTCTG 
GACAAAGAGG AGGACGTTTA CGAAGTCCTA CCAGTGGACT ATGCCGATTA TTACCAGGGC 
CTCTGGGATT GCCATGGTGA CCAGCCAGAT GAACTGTCCT TCCAACGAGG TGACCTCATC 
CGCATTCTGA GCAAGGAGTA CAACATGTAC GGCTGGTGGG TAGGAGAACT GAACAGCATC 
ATTGGGATTG TTCCAAAGGA CTACCTCACC ACCGCCTTTG AAATGGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452542.1
CDS	150..1166
Translation	MQAIALPEEICWLLEDAEDFLAEGLQNENLSPGAQDQRDHILRGFQQIKSRYCWDFQPQGDDLGQDGSDENLSGTHGPALASDASFWSDYQEEGIDDIIRGAQELDNVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGYNVRMAPHLRKDSKKDSCFELTSQDRRSYEFTAFSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEDKEQEEMYNDIDGFDSARSGSQGRAMALPEPLDKEEDVYEVLPVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSIIGIVPKDYLTTAFEMEGR

Target ORF information:

RefSeq Version	XM_017597053.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus src kinase associated phosphoprotein 1 (Skap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597053.1

ORF Insert Sequence:

ATGCAGGCCA TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATGC TGAAGACTTT 
CTGGCGGAAG GCTTGCAGAA TGAGAACCTT AGTCCTGGGG CACAGGATCA GAGAGACCAC 
ATTCTCCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GACGACCTTG GCCAAGACGG CTCTGATGAA AATCTGAGTG GGACACACGG CCCAGCCCTC 
GCATCAGATG CATCCTTTTG GTCAGATTAT CAGGAGGAGG GAATAGACGA CATCATCAGA 
GGGGCTCAAG AGCTGGATAA CGTCATCAAA CAAGGATACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGATGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGAA CCTTCCTCAT TAAGGGATAC 
AACGTCCGGA TGGCCCCTCA CCTGCGGAAA GACTCCAAGA AAGACTCCTG CTTTGAACTG 
ACCTCCCAGG ATAGGCGCAG CTATGAGTTC ACAGCTTTTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTGACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAGGAAGA GGAAGACAAA GAACAGGAAG AAATGTACAA TGACATTGAT 
GGCTTTGACT CCGCAAGGTC CGGCTCCCAG GGCAGAGCCA TGGCCTTGCC TGAGCCTCTG 
GACAAAGAGG AGGACGTTTA CGAAGTCCTA CCAGTGGACT ATGCCGATTA TTACCAGGGC 
CTCTGGGATT GCCATGGTGA CCAGCCAGAT GAACTGTCCT TCCAACGAGG TGACCTCATC 
CGCATTCTGA GCAAGGAGTA CAACATGTAC GGCTGGTGGG TAGGAGAACT GAACAGCATC 
ATTGGGATTG TTCCAAAGGA CTACCTCACC ACCGCCTTTG AAATGGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Signaling Scaffold Protein IQGAP1 Interacts with Microtubule Plus-end Tracking Protein SKAP and Links Dynamic Microtubule Plus-end to Steer Cell Migration.
The Journal of biological chemistry290(39)23766-80(2015 Sep)
Cao D,Su Z,Wang W,Wu H,Liu X,Akram S,Qin B,Zhou J,Zhuang X,Adams G,Jin C,Wang X,Liu L,Hill DL,Wang D,Ding X,Yao X

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M

RIAM links the ADAP/SKAP-55 signaling module to Rap1, facilitating T-cell-receptor-mediated integrin activation.
Molecular and cellular biology27(11)4070-81(2007 Jun)
M?nasch? G,Kliche S,Chen EJ,Stradal TE,Schraven B,Koretzky G

Targeting of MIST to Src-family kinases via SKAP55-SLAP-130 adaptor complex in mast cells.
FEBS letters540(1-3)111-6(2003 Apr)
Fujii Y,Wakahara S,Nakao T,Hara T,Ohtake H,Komurasaki T,Kitamura K,Tatsuno A,Fujiwara N,Hozumi N,Ra C,Kitamura D,Goitsuka R

SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates.
Nature immunology4(4)366-74(2003 Apr)
Wang H,Moon EY,Azouz A,Wu X,Smith A,Schneider H,Hogg N,Rudd CE

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Skap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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