Skap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Skap1
Entrez Gene ID	78473
Full Name	src family associated phosphoprotein 1
Synonyms	1700091G21Rik,Scap1,Skap-55
General protein information	Preferred Names src family associated phosphoprotein 1 Names src kinase-associated phosphoprotein 1 ortholog of human src family associated phosphoprotein 1 SCAP1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_030246431.1	XP_030102291.1	src kinase-associated phosphoprotein 1 isoform X4
NM_001177898.1	NP_001171369.1	src kinase-associated phosphoprotein 1 isoform 2
NM_001177899.1	NP_001171370.1	src kinase-associated phosphoprotein 1 isoform 3
NM_001033186.3	NP_001028358.1	src kinase-associated phosphoprotein 1 isoform 1
XM_006534508.3	XP_006534571.1	src kinase-associated phosphoprotein 1 isoform X1
XM_017314838.1	XP_017170327.1	src kinase-associated phosphoprotein 1 isoform X2
XM_017314839.1	XP_017170328.1	src kinase-associated phosphoprotein 1 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	skap1	NP_001073480.1
Xenopus tropicalis (tropical clawed frog)	skap1	NP_001004990.1
Mus musculus (house mouse)	Skap1	NP_001028358.1
Canis lupus familiaris (dog)	SKAP1	XP_537665.3
Rattus norvegicus (Norway rat)	Skap1	NP_775433.2
Homo sapiens (human)	SKAP1	NP_003717.3
Pan troglodytes (chimpanzee)	SKAP1	XP_523821.2
Bos taurus (cattle)	SKAP1	XP_002695990.2

Gene Ontology
Bibliography

Related articles in PubMed

T-cell immune adaptor SKAP1 regulates the induction of collagen-induced arthritis in mice.
Smith X, Taylor A, Rudd CE
Immunology letters176122-7(2016 08)

Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1.
Lim D, Lu Y, Rudd CE
Immunology letters17240-6(2016 04)

ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy.
Li C, Li W, Xiao J, Jiao S, Teng F, Xue S, Zhang C, Sheng C, Leng Q, Rudd CE, Wei B, Wang H
EMBO molecular medicine7(6)754-69(2015 Jun)

Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.
Ayoub E, Hall A, Scott AM, Chagnon MJ, Miquel G, Hall? M, Noda M, Bikfalvi A, Tremblay ML
The Journal of biological chemistry288(36)25739-48(2013 Sep)

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, W?lfing C, Malissen B, Malissen M
Nature immunology14(8)858-66(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that Skap1-deficient (skap1-/-) mice are highly resistant to the induction of collagen-induced arthritis
Title: T-cell immune adaptor SKAP1 regulates the induction of collagen-induced arthritis in mice.

SKAP1 can affect the function of talin in T-cells needed for optimal T-cell/dendritic cell conjugation
Title: Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1.

In this study, the authors identified that the ADAP-SKAP55 signaling module reduced CD8(+) cytotoxic T lymphocyte cytotoxicity and enhanced PD-1 expression in a Fyn-, Ca(2+)-, and NFATc1-dependent manner.
Title: ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy.

Data suggest that the presence or absence of associated SKAP55 defines functionally distinct pools of ADAP.
Title: The pleckstrin homology domain in the SKAP55 adapter protein defines the ability of the adapter protein ADAP to regulate integrin function and NF-kappaB activation.

HPK1 associates with SKAP1 to negatively regulate Rap1-mediated B-lymphocyte adhesion.
Title: HPK1 associates with SKAP-HOM to negatively regulate Rap1-mediated B-lymphocyte adhesion.

The following Skap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Skap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15558	NM_001033186.3 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu15642	NM_001177898.1 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu15707	NM_001177899.1 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu47122	XM_006534508.3 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu78083	XM_017314838.1 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu78084	XM_017314839.1 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu85948	XM_030246431.1 Latest version!	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Skap1 ( NM_001033186.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001028358.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15558
Clone ID Related Accession (Same CDS sequence)	NM_001033186.3
Accession Version	NM_001033186.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK040068.1 and AK137942.1. On May 1, 2010 this sequence version replaced NM_001033186.2. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK137942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGATG ATGATGATCT AGAAGAGGAT 
ACATGTGGCG CTCATCGGCG ACGAGTGGAC TATGCCGATT ATTACCAGGG CCTATGGGAT 
TGCCATGGTG ACCAGCCGGA TGAACTGTCC TTCCAACGGG GTGACCTCAT CCGCATTCTG 
AGCAAGGAGT ACAACATGTA CGGCTGGTGG GTAGGAGAGC TGAACAGCGT CATTGGAATT 
GTTCCAAAGG ACTATCTCAC CACCGCCTTT GAAATGGAAG GAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028358.1
CDS	115..1182
Translation	MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRGFQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGIEDILRGAQELDSVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGYSVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPDDDDLEEDTCGAHRRRVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSVIGIVPKDYLTTAFEMEGI

Target ORF information:

RefSeq Version	NM_001033186.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033186.3

ORF Insert Sequence:

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGATG ATGATGATCT AGAAGAGGAT 
ACATGTGGCG CTCATCGGCG ACGAGTGGAC TATGCCGATT ATTACCAGGG CCTATGGGAT 
TGCCATGGTG ACCAGCCGGA TGAACTGTCC TTCCAACGGG GTGACCTCAT CCGCATTCTG 
AGCAAGGAGT ACAACATGTA CGGCTGGTGG GTAGGAGAGC TGAACAGCGT CATTGGAATT 
GTTCCAAAGG ACTATCTCAC CACCGCCTTT GAAATGGAAG GAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( NM_001177898.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171369.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15642
Clone ID Related Accession (Same CDS sequence)	NM_001177898.1
Accession Version	NM_001177898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK040068.1, AK138030.1 and AK137942.1. Transcript Variant: This variant (2) lacks two alternate exons, one of which changes the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK138030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGTGG ACTATGCCGA TTATTACCAG 
GGCCTATGGG ATTGCCATGG TGACCAGCCG GATGAACTGT CCTTCCAACG GGGTGACCTC 
ATCCGCATTC TGAGCAAGGG AACATATTCT CAGACCATCA GAAACTCCCG TCCCCTACTC 
TGGCCATGGA TCCTTCTCTT CCCTGAATGG AGCCAAGACT CTGCTGCTTC CTGTGACTTT 
AAGCCACTGA TAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171369.1
CDS	115..1152
Translation	MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRGFQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGIEDILRGAQELDSVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGYSVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKGTYSQTIRNSRPLLWPWILLFPEWSQDSAASCDFKPLIR

Target ORF information:

RefSeq Version	NM_001177898.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177898.1

ORF Insert Sequence:

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGTGG ACTATGCCGA TTATTACCAG 
GGCCTATGGG ATTGCCATGG TGACCAGCCG GATGAACTGT CCTTCCAACG GGGTGACCTC 
ATCCGCATTC TGAGCAAGGG AACATATTCT CAGACCATCA GAAACTCCCG TCCCCTACTC 
TGGCCATGGA TCCTTCTCTT CCCTGAATGG AGCCAAGACT CTGCTGCTTC CTGTGACTTT 
AAGCCACTGA TAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( NM_001177899.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171370.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu15707
Clone ID Related Accession (Same CDS sequence)	NM_001177899.1
Accession Version	NM_001177899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK040068.1, BY723539.1, AK137942.1 and BC150764.1. Transcript Variant: This variant (3) lacks an alternate, in-frame exon, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145402.1, AK169825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGTGG ACTATGCCGA TTATTACCAG 
GGCCTATGGG ATTGCCATGG TGACCAGCCG GATGAACTGT CCTTCCAACG GGGTGACCTC 
ATCCGCATTC TGAGCAAGGA GTACAACATG TACGGCTGGT GGGTAGGAGA GCTGAACAGC 
GTCATTGGAA TTGTTCCAAA GGACTATCTC ACCACCGCCT TTGAAATGGA AGGAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171370.1
CDS	115..1134
Translation	MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRGFQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGIEDILRGAQELDSVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGYSVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSVIGIVPKDYLTTAFEMEGI

Target ORF information:

RefSeq Version	NM_001177899.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177899.1

ORF Insert Sequence:

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGTGG ACTATGCCGA TTATTACCAG 
GGCCTATGGG ATTGCCATGG TGACCAGCCG GATGAACTGT CCTTCCAACG GGGTGACCTC 
ATCCGCATTC TGAGCAAGGA GTACAACATG TACGGCTGGT GGGTAGGAGA GCTGAACAGC 
GTCATTGGAA TTGTTCCAAA GGACTATCTC ACCACCGCCT TTGAAATGGA AGGAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( XM_006534508.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534571.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47122
Clone ID Related Accession (Same CDS sequence)	XM_006534508.3
Accession Version	XM_006534508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGATG ATGATGATCT AGAAGAGGAT 
ACATGTGGCG CTCATCGGCG ACGAGTGGAC TATGCCGATT ATTACCAGGG CCTATGGGAT 
TGCCATGGTG ACCAGCCGGA TGAACTGTCC TTCCAACGGG GTGACCTCAT CCGCATTCTG 
AGCAAGGGAA CATATTCTCA GACCATCAGA AACTCCCGTC CCCTACTCTG GCCATGGATC 
CTTCTCTTCC CTGAATGGAG CCAAGACTCT GCTGCTTCCT GTGACTTTAA GCCACTGATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534571.1
CDS	136..1221
Translation	MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRGFQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGIEDILRGAQELDSVIKQGYLEKKSKDHSFFGSEWQKRWCVISRGLFLYYANEKSKQPKGTFLIKGYSVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPDDDDLEEDTCGAHRRRVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKGTYSQTIRNSRPLLWPWILLFPEWSQDSAASCDFKPLIR

Target ORF information:

RefSeq Version	XM_006534508.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534508.3

ORF Insert Sequence:

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GAATAGAAGA CATCTTAAGA 
GGGGCTCAAG AGCTGGATAG CGTCATCAAG CAAGGGTACC TGGAGAAGAA AAGCAAAGAT 
CATAGCTTCT TTGGGTCGGA GTGGCAGAAG CGCTGGTGTG TTATCAGCAG AGGGCTCTTC 
CTCTACTATG CTAATGAGAA AAGCAAGCAG CCCAAAGGGA CCTTCCTCAT TAAGGGCTAC 
AGTGTCCGGA TGGCCCCTCA CCTGCGAAAA GATTCCAAGA AAGAATCCTG CTTTGAACTG 
ATCTCCCAGG ATAGGCGCAG CTATGAGTTT ACAGCTTCTA GTCCAGCTGA AGCCAGAGAC 
TGGGTGGATC AAATAAGTTT CTTGTTAAAA GATCTTAGCT CCTTAACCAT TCCCTTCGAA 
GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGAAGAGG AAGAAATGTA CAATGACGTC 
GATGGCTTTG ACTCCCCAAG GTCTGGCTCC CAGTGCAGAG CCATGGCCTT GCCTGAGCCT 
ACTGAGAAAG AGGAGGACAT TTATGAAGTC CTACCAGATG ATGATGATCT AGAAGAGGAT 
ACATGTGGCG CTCATCGGCG ACGAGTGGAC TATGCCGATT ATTACCAGGG CCTATGGGAT 
TGCCATGGTG ACCAGCCGGA TGAACTGTCC TTCCAACGGG GTGACCTCAT CCGCATTCTG 
AGCAAGGGAA CATATTCTCA GACCATCAGA AACTCCCGTC CCCTACTCTG GCCATGGATC 
CTTCTCTTCC CTGAATGGAG CCAAGACTCT GCTGCTTCCT GTGACTTTAA GCCACTGATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( XM_017314838.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170327.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X2, mRNA.

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CloneID	OMu78083
Clone ID Related Accession (Same CDS sequence)	XM_017314838.1
Accession Version	XM_017314838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GCAAGCAGCC CAAAGGGACC 
TTCCTCATTA AGGGCTACAG TGTCCGGATG GCCCCTCACC TGCGAAAAGA TTCCAAGAAA 
GAATCCTGCT TTGAACTGAT CTCCCAGGAT AGGCGCAGCT ATGAGTTTAC AGCTTCTAGT 
CCAGCTGAAG CCAGAGACTG GGTGGATCAA ATAAGTTTCT TGTTAAAAGA TCTTAGCTCC 
TTAACCATTC CCTTCGAAGA GGAGGAGGAG GAAGAGGAGG AAGAAGAGAA AGAAGAGGAA 
GAAATGTACA ATGACGTCGA TGGCTTTGAC TCCCCAAGGT CTGGCTCCCA GTGCAGAGCC 
ATGGCCTTGC CTGAGCCTAC TGAGAAAGAG GAGGACATTT ATGAAGTCCT ACCAGATGAT 
GATGATCTAG AAGAGGATAC ATGTGGCGCT CATCGGCGAC GAGTGGACTA TGCCGATTAT 
TACCAGGGCC TATGGGATTG CCATGGTGAC CAGCCGGATG AACTGTCCTT CCAACGGGGT 
GACCTCATCC GCATTCTGAG CAAGGGAACA TATTCTCAGA CCATCAGAAA CTCCCGTCCC 
CTACTCTGGC CATGGATCCT TCTCTTCCCT GAATGGAGCC AAGACTCTGC TGCTTCCTGT 
GACTTTAAGC CACTGATAAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170327.1
CDS	136..1059
Translation	MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRGFQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGKQPKGTFLIKGYSVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPDDDDLEEDTCGAHRRRVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKGTYSQTIRNSRPLLWPWILLFPEWSQDSAASCDFKPLIR

Target ORF information:

RefSeq Version	XM_017314838.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314838.1

ORF Insert Sequence:

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GCAAGCAGCC CAAAGGGACC 
TTCCTCATTA AGGGCTACAG TGTCCGGATG GCCCCTCACC TGCGAAAAGA TTCCAAGAAA 
GAATCCTGCT TTGAACTGAT CTCCCAGGAT AGGCGCAGCT ATGAGTTTAC AGCTTCTAGT 
CCAGCTGAAG CCAGAGACTG GGTGGATCAA ATAAGTTTCT TGTTAAAAGA TCTTAGCTCC 
TTAACCATTC CCTTCGAAGA GGAGGAGGAG GAAGAGGAGG AAGAAGAGAA AGAAGAGGAA 
GAAATGTACA ATGACGTCGA TGGCTTTGAC TCCCCAAGGT CTGGCTCCCA GTGCAGAGCC 
ATGGCCTTGC CTGAGCCTAC TGAGAAAGAG GAGGACATTT ATGAAGTCCT ACCAGATGAT 
GATGATCTAG AAGAGGATAC ATGTGGCGCT CATCGGCGAC GAGTGGACTA TGCCGATTAT 
TACCAGGGCC TATGGGATTG CCATGGTGAC CAGCCGGATG AACTGTCCTT CCAACGGGGT 
GACCTCATCC GCATTCTGAG CAAGGGAACA TATTCTCAGA CCATCAGAAA CTCCCGTCCC 
CTACTCTGGC CATGGATCCT TCTCTTCCCT GAATGGAGCC AAGACTCTGC TGCTTCCTGT 
GACTTTAAGC CACTGATAAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( XM_017314839.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170328.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu78084
Clone ID Related Accession (Same CDS sequence)	XM_017314839.1
Accession Version	XM_017314839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GCAAGCAGCC CAAAGGGACC 
TTCCTCATTA AGGGCTACAG TGTCCGGATG GCCCCTCACC TGCGAAAAGA TTCCAAGAAA 
GAATCCTGCT TTGAACTGAT CTCCCAGGAT AGGCGCAGCT ATGAGTTTAC AGCTTCTAGT 
CCAGCTGAAG CCAGAGACTG GGTGGATCAA ATAAGTTTCT TGTTAAAAGA TCTTAGCTCC 
TTAACCATTC CCTTCGAAGA GGAGGAGGAG GAAGAGGAGG AAGAAGAGAA AGAAGAGGAA 
GAAATGTACA ATGACGTCGA TGGCTTTGAC TCCCCAAGGT CTGGCTCCCA GTGCAGAGCC 
ATGGCCTTGC CTGAGCCTAC TGAGAAAGAG GAGGACATTT ATGAAGTCCT ACCAGTGGAC 
TATGCCGATT ATTACCAGGG CCTATGGGAT TGCCATGGTG ACCAGCCGGA TGAACTGTCC 
TTCCAACGGG GTGACCTCAT CCGCATTCTG AGCAAGGGAA CATATTCTCA GACCATCAGA 
AACTCCCGTC CCCTACTCTG GCCATGGATC CTTCTCTTCC CTGAATGGAG CCAAGACTCT 
GCTGCTTCCT GTGACTTTAA GCCACTGATA AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170328.1
CDS	136..1011
Translation	MQAVALPEEICWLLEDTEDFLAEGLQNENLSPGAQDQRAHILRGFQQIKSRYCWDFQPQGGDLGQDGSDDNLSGTHGPPLTSEASFWSDYQDEGKQPKGTFLIKGYSVRMAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKGTYSQTIRNSRPLLWPWILLFPEWSQDSAASCDFKPLIR

Target ORF information:

RefSeq Version	XM_017314839.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314839.1

ORF Insert Sequence:

ATGCAGGCTG TCGCCCTCCC GGAGGAGATC TGTTGGCTCC TGGAAGATAC CGAAGACTTC 
CTGGCAGAAG GCTTGCAGAA TGAGAACCTC AGCCCTGGGG CACAGGATCA GAGAGCCCAC 
ATTCTTCGGG GTTTCCAGCA AATCAAATCC AGGTACTGTT GGGATTTTCA GCCCCAAGGA 
GGTGACCTTG GCCAGGACGG CTCTGATGAC AATCTGAGTG GGACTCACGG CCCACCCCTC 
ACGTCAGAAG CATCCTTTTG GTCAGATTAT CAGGACGAGG GCAAGCAGCC CAAAGGGACC 
TTCCTCATTA AGGGCTACAG TGTCCGGATG GCCCCTCACC TGCGAAAAGA TTCCAAGAAA 
GAATCCTGCT TTGAACTGAT CTCCCAGGAT AGGCGCAGCT ATGAGTTTAC AGCTTCTAGT 
CCAGCTGAAG CCAGAGACTG GGTGGATCAA ATAAGTTTCT TGTTAAAAGA TCTTAGCTCC 
TTAACCATTC CCTTCGAAGA GGAGGAGGAG GAAGAGGAGG AAGAAGAGAA AGAAGAGGAA 
GAAATGTACA ATGACGTCGA TGGCTTTGAC TCCCCAAGGT CTGGCTCCCA GTGCAGAGCC 
ATGGCCTTGC CTGAGCCTAC TGAGAAAGAG GAGGACATTT ATGAAGTCCT ACCAGTGGAC 
TATGCCGATT ATTACCAGGG CCTATGGGAT TGCCATGGTG ACCAGCCGGA TGAACTGTCC 
TTCCAACGGG GTGACCTCAT CCGCATTCTG AGCAAGGGAA CATATTCTCA GACCATCAGA 
AACTCCCGTC CCCTACTCTG GCCATGGATC CTTCTCTTCC CTGAATGGAG CCAAGACTCT 
GCTGCTTCCT GTGACTTTAA GCCACTGATA AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Skap1 ( XM_030246431.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102291.1 Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu85948
Clone ID Related Accession (Same CDS sequence)	XM_030246431.1
Accession Version	XM_030246431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	src kinase-associated phosphoprotein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTC ACCTGCGAAA AGATTCCAAG AAAGAATCCT GCTTTGAACT GATCTCCCAG 
GATAGGCGCA GCTATGAGTT TACAGCTTCT AGTCCAGCTG AAGCCAGAGA CTGGGTGGAT 
CAAATAAGTT TCTTGTTAAA AGATCTTAGC TCCTTAACCA TTCCCTTCGA AGAGGAGGAG 
GAGGAAGAGG AGGAAGAAGA GAAAGAAGAG GAAGAAATGT ACAATGACGT CGATGGCTTT 
GACTCCCCAA GGTCTGGCTC CCAGTGCAGA GCCATGGCCT TGCCTGAGCC TACTGAGAAA 
GAGGAGGACA TTTATGAAGT CCTACCAGAT GATGATGATC TAGAAGAGGA TACATGTGGC 
GCTCATCGGC GACGAGTGGA CTATGCCGAT TATTACCAGG GCCTATGGGA TTGCCATGGT 
GACCAGCCGG ATGAACTGTC CTTCCAACGG GGTGACCTCA TCCGCATTCT GAGCAAGGGA 
ACATATTCTC AGACCATCAG AAACTCCCGT CCCCTACTCT GGCCATGGAT CCTTCTCTTC 
CCTGAATGGA GCCAAGACTC TGCTGCTTCC TGTGACTTTA AGCCACTGAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102291.1
CDS	537..1133
Translation	MAPHLRKDSKKESCFELISQDRRSYEFTASSPAEARDWVDQISFLLKDLSSLTIPFEEEEEEEEEEEKEEEEMYNDVDGFDSPRSGSQCRAMALPEPTEKEEDIYEVLPDDDDLEEDTCGAHRRRVDYADYYQGLWDCHGDQPDELSFQRGDLIRILSKGTYSQTIRNSRPLLWPWILLFPEWSQDSAASCDFKPLIR

Target ORF information:

RefSeq Version	XM_030246431.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus src family associated phosphoprotein 1 (Skap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246431.1

ORF Insert Sequence:

ATGGCCCCTC ACCTGCGAAA AGATTCCAAG AAAGAATCCT GCTTTGAACT GATCTCCCAG 
GATAGGCGCA GCTATGAGTT TACAGCTTCT AGTCCAGCTG AAGCCAGAGA CTGGGTGGAT 
CAAATAAGTT TCTTGTTAAA AGATCTTAGC TCCTTAACCA TTCCCTTCGA AGAGGAGGAG 
GAGGAAGAGG AGGAAGAAGA GAAAGAAGAG GAAGAAATGT ACAATGACGT CGATGGCTTT 
GACTCCCCAA GGTCTGGCTC CCAGTGCAGA GCCATGGCCT TGCCTGAGCC TACTGAGAAA 
GAGGAGGACA TTTATGAAGT CCTACCAGAT GATGATGATC TAGAAGAGGA TACATGTGGC 
GCTCATCGGC GACGAGTGGA CTATGCCGAT TATTACCAGG GCCTATGGGA TTGCCATGGT 
GACCAGCCGG ATGAACTGTC CTTCCAACGG GGTGACCTCA TCCGCATTCT GAGCAAGGGA 
ACATATTCTC AGACCATCAG AAACTCCCGT CCCCTACTCT GGCCATGGAT CCTTCTCTTC 
CCTGAATGGA GCCAAGACTC TGCTGCTTCC TGTGACTTTA AGCCACTGAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

T-cell immune adaptor SKAP1 regulates the induction of collagen-induced arthritis in mice.
Immunology letters176122-7(2016 08)
Smith X,Taylor A,Rudd CE

Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1.
Immunology letters17240-6(2016 04)
Lim D,Lu Y,Rudd CE

ADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy.
EMBO molecular medicine7(6)754-69(2015 Jun)
Li C,Li W,Xiao J,Jiao S,Teng F,Xue S,Zhang C,Sheng C,Leng Q,Rudd CE,Wei B,Wang H

Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility.
The Journal of biological chemistry288(36)25739-48(2013 Sep)
Ayoub E,Hall A,Scott AM,Chagnon MJ,Miquel G,Hall? M,Noda M,Bikfalvi A,Tremblay ML

The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells.
Nature immunology14(8)858-66(2013 Aug)
Liang Y,Cucchetti M,Roncagalli R,Yokosuka T,Malzac A,Bertosio E,Imbert J,Nijman IJ,Suchanek M,Saito T,W?lfing C,Malissen B,Malissen M

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Skap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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