Npb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npb
Entrez Gene ID	259222
Full Name	neuropeptide B
General protein information	Preferred Names neuropeptide B Names neuropeptide B preproneuropeptide B preproprotein L7 rPPL7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3
Summary	ligand for G protein-coupled receptor (GPR7) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153293.1	NP_695205.1	neuropeptide B precursor

Pathways from BioSystems

Homologies

	NPB	XP_001112472.1
Bos taurus (cattle)	NPB	NP_776369.1
Mus musculus (house mouse)	Npb	NP_695020.1
Rattus norvegicus (Norway rat)	Npb	NP_695205.1
Homo sapiens (human)	NPB	NP_683694.1
Canis lupus familiaris (dog)	NPB	XP_003639281.1

Gene Ontology
Bibliography

Related articles in PubMed

Neuropeptide B and W regulate leptin and resistin secretion, and stimulate lipolysis in isolated rat adipocytes.
Skrzypski M, Pruszy?ska-Oszma?ek E, Ruci?ski M, Szczepankiewicz D, Sassek M, Wojciechowicz T, Kaczmarek P, Ko?odziejski PA, Strowski MZ, Malendowicz LK, Nowak KW
Regulatory peptides176(1-3)51-6(2012 Jun)

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells.
Ziolkowska A, Rucinski M, Tyczewska M, Malendowicz LK
International journal of molecular medicine24(6)781-7(2009 Dec)

Immunofluorescent identification of neuropeptide B-containing nerve fibers and terminals in the rat hypothalamus.
Schulz S, Stumm R, H?llt V
Neuroscience letters411(1)67-71(2007 Jan)

A study of the rat neuropeptide B/neuropeptide W system using in situ techniques.
Jackson VR, Lin SH, Wang Z, Nothacker HP, Civelli O
The Journal of comparative neurology497(3)367-83(2006 Jul)

Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7.
Fujii R, Yoshida H, Fukusumi S, Habata Y, Hosoya M, Kawamata Y, Yano T, Hinuma S, Kitada C, Asami T, Mori M, Fujisawa Y, Fujino M
The Journal of biological chemistry277(37)34010-6(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Neuropeptide B can affect glucose and lipid homeostasis, and food intake in rats.
Title: Neuropeptide B and W regulate leptin and resistin secretion, and stimulate lipolysis in isolated rat adipocytes.

RT-PCR technique revealed the presence of NPB mRNA, NPW mRNA, and NPBWR1 mRNA, but not similar to NPBWR2 mRNA in ROB cells.
Title: Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells.

NPB-immunoreactivity was enriched in many regions within the hypothalamus which also contained high levels of GPR7 mRNA.
Title: Immunofluorescent identification of neuropeptide B-containing nerve fibers and terminals in the rat hypothalamus.

demonstrates the expression patterns of GPR7, NPB, and NPW precursor messenger ribonucleic acid (mRNA) in the rat brain by using in situ hybridization and in situ binding experiments
Title: A study of the rat neuropeptide B/neuropeptide W system using in situ techniques.

neuropeptide B, a neuropeptide modified with bromine is an endogenous ligand for GPR7.
Title: Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7.

The following Npb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02838	NM_153293.1 Latest version!	Rattus norvegicus neuropeptide B (Npb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npb ( NM_153293.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_695205.1 Rattus norvegicus neuropeptide B (Npb), mRNA.

Price & Availability↑

CloneID	ORa02838
Clone ID Related Accession (Same CDS sequence)	NM_153293.1
Accession Version	NM_153293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB085944.1. ##Evidence-Data-START## Transcript exon combination :: AB085944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689603, SAMN00632258 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCGGT GTAGGACGCT GGTGGCCGCC GCCCTGGCGC TGCTCCTGAC GCCAGCCCTC 
GCGTGGTACA AGCCCGCGGC GGGATCCCAC CACTACTCGG TGGGCCGCGC TGCGGGGCTA 
CTGTCGAGTT TCCACAGGTT CCCATCCACG CGACGTTCCG AGTCTCCAGC ACTCCGGGTG 
GGAACCGTAC CTCTGCGCAA CTTGGAGATG CGCCCAAGCG TAAGAAGCCT TGCCCTGTGT 
GTCAAAGATG TGACCCCGAA CCTGCAGAGC TGCCAGCGGC AACTCAACAG CCGAGGGACT 
TTCCAGTGTA AGGCGGACGT CTTCTTGTCG CTGCACAAGG CTGAATGCCA AAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_695205.1
CDS	1..360
Translation	MVRCRTLVAAALALLLTPALAWYKPAAGSHHYSVGRAAGLLSSFHRFPSTRRSESPALRVGTVPLRNLEMRPSVRSLALCVKDVTPNLQSCQRQLNSRGTFQCKADVFLSLHKAECQSA

Target ORF information:

RefSeq Version	NM_153293.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide B (Npb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153293.1

ORF Insert Sequence:

ATGGTCCGGT GTAGGACGCT GGTGGCCGCC GCCCTGGCGC TGCTCCTGAC GCCAGCCCTC 
GCGTGGTACA AGCCCGCGGC GGGATCCCAC CACTACTCGG TGGGCCGCGC TGCGGGGCTA 
CTGTCGAGTT TCCACAGGTT CCCATCCACG CGACGTTCCG AGTCTCCAGC ACTCCGGGTG 
GGAACCGTAC CTCTGCGCAA CTTGGAGATG CGCCCAAGCG TAAGAAGCCT TGCCCTGTGT 
GTCAAAGATG TGACCCCGAA CCTGCAGAGC TGCCAGCGGC AACTCAACAG CCGAGGGACT 
TTCCAGTGTA AGGCGGACGT CTTCTTGTCG CTGCACAAGG CTGAATGCCA AAGCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neuropeptide B and W regulate leptin and resistin secretion, and stimulate lipolysis in isolated rat adipocytes.
Regulatory peptides176(1-3)51-6(2012 Jun)
Skrzypski M,Pruszy?ska-Oszma?ek E,Ruci?ski M,Szczepankiewicz D,Sassek M,Wojciechowicz T,Kaczmarek P,Ko?odziejski PA,Strowski MZ,Malendowicz LK,Nowak KW

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells.
International journal of molecular medicine24(6)781-7(2009 Dec)
Ziolkowska A,Rucinski M,Tyczewska M,Malendowicz LK

Immunofluorescent identification of neuropeptide B-containing nerve fibers and terminals in the rat hypothalamus.
Neuroscience letters411(1)67-71(2007 Jan)
Schulz S,Stumm R,H?llt V

A study of the rat neuropeptide B/neuropeptide W system using in situ techniques.
The Journal of comparative neurology497(3)367-83(2006 Jul)
Jackson VR,Lin SH,Wang Z,Nothacker HP,Civelli O

Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7.
The Journal of biological chemistry277(37)34010-6(2002 Sep)
Fujii R,Yoshida H,Fukusumi S,Habata Y,Hosoya M,Kawamata Y,Yano T,Hinuma S,Kitada C,Asami T,Mori M,Fujisawa Y,Fujino M

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Npb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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