Ntf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ntf4
Entrez Gene ID	25730
Full Name	neurotrophin 4
Synonyms	NT4P,Ntf5
General protein information	Preferred Names neurotrophin 4 Names neurotrophin-4 NT-4 NT-5 Neurotrophin 5 (neurotrophin 4/5) neurotrophin-5 neutrophic factor 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	promotes the survival of peripheral sensory and sympathetic neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013184.3	NP_037316.2	neurotrophin-4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	NTF4	NP_006170.1
Mus musculus (house mouse)	Ntf5	NP_937833.1
	NTF4	XP_001114610.1
Canis lupus familiaris (dog)	NTF4	NP_001177358.1
Bos taurus (cattle)	NTF4	XP_002695248.2
Xenopus tropicalis (tropical clawed frog)	ntf4	XP_002939282.1
Pan troglodytes (chimpanzee)	NTF4	XP_001172220.1
Rattus norvegicus (Norway rat)	Ntf4	NP_037316.2

Gene Ontology
Bibliography

Related articles in PubMed

BDNF and NT4 play interchangeable roles in gustatory development.
Huang T, Krimm RF
Developmental biology386(2)308-20(2014 Feb)

Increased expression of neurotrophin 4 following focal cerebral ischemia in adult rat brain with treadmill exercise.
Chung JY, Kim MW, Bang MS, Kim M
PloS one8(3)e52461(2013)

Effect of neurotrophin-4 on endoplasmic reticulum stress-related neuronal apoptosis in diabetic and high glucose exposed rat retinas.
Oshitari T, Yoshida-Hata N, Yamamoto S
Neuroscience letters501(2)102-6(2011 Aug)

Chronic ecstasy use increases neurotrophin-4 gene expression and protein levels in the rat brain.
Hatami H, Hossainpour-Faizi MA, Azarfarin M, Azarfam P
Pharmacological reports : PR62(6)998-1004(2010 Nov-Dec)

Deficits in LTP and recognition memory in the genetically hypertensive rat are associated with decreased expression of neurotrophic factors and their receptors in the dentate gyrus.
Hennigan A, Callaghan CK, Kealy J, Rouine J, Kelly AM
Behavioural brain research197(2)371-7(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In a permanent middle cerebral artery occlusion model, ischemia decreased NT-4 and trkB expression. Immunohistochemistry showed their immunoreactivities around the region of the ischemic area.
Title: Increased expression of neurotrophin 4 following focal cerebral ischemia in adult rat brain with treadmill exercise.

The neuroprotective effect of NT-4 on retinas cultured under diabetic stress correlates with suppression of PERK kinase and transcription factor CHOP.
Title: Effect of neurotrophin-4 on endoplasmic reticulum stress-related neuronal apoptosis in diabetic and high glucose exposed rat retinas.

alteration of NT-4 protein levels and gene expression in brain stem, cerebellum and cerebral hemisphere in context of ecstasy dependence; results show chronic administration of ecstasy significantly increased NT-4 gene expression in these areas of brain
Title: Chronic ecstasy use increases neurotrophin-4 gene expression and protein levels in the rat brain.

Analysis of neurotrophin expression in dentate gyrus dosen't reveal an expression change of Ntf4 in a genetically hypertensive strain of Wistar rat.
Title: Deficits in LTP and recognition memory in the genetically hypertensive rat are associated with decreased expression of neurotrophic factors and their receptors in the dentate gyrus.

NT-4 regulates proliferation and differentiation of the dental epithelium and promotes production of the enamel matrix
Title: Neurotrophic factor neurotrophin-4 regulates ameloblastin expression via full-length TrkB.

The following Ntf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ntf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02248	NM_013184.3 Latest version!	Rattus norvegicus neurotrophin 4 (Ntf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ntf4 ( NM_013184.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037316.2 Rattus norvegicus neurotrophin 4 (Ntf4), mRNA.

Price & Availability↑

CloneID	ORa02248
Clone ID Related Accession (Same CDS sequence)	NM_013184.3
Accession Version	NM_013184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	neurotrophin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S69323.1. On Jun 11, 2003 this sequence version replaced NM_013184.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S69323.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCCTC GCCACTCCTG CTCTCTCCTC CTTTTCCTTC TCCTCCTCCC CAGCGTCCCC 
ATGGAGCCCC AACCCCCCTC CTCCACCCTG CCCCCTTTTC TGGCTCCTGA GTGGGACCTC 
TTGTCTCCCC GAGTGGCCCT GTCAAGGGGC ACCCCTGCCG GGCCCCCTCT CCTCTTCCTG 
CTGGAGGCCG GGGCCTATGG GGAGCCGGCA GGGGCCCCAG CCAACCGCAG CCGGCGCGGG 
GTGAGTGAGA CGGCACCGGC AAGCCGCCGG GGTGAGCTGG CAGTGTGCGA TGCAGTGAGT 
GGCTGGGTGA CCGACCGGCG GACAGCTGTG GACTTGCGTG GGCGCGAGGT GGAGGTGCTG 
GGCGAGGTGC CTGCGGCAGG TGGCAGTCCC CTGCGTCAGT ACTTCTTCGA GACGCGCTGC 
AAGGCCGAAA GCGCTGGGGA AGGTGGCCCA GGTGTGGGCG GAGGGGGCTG TCGCGGCGTG 
GATCGGAGGC ACTGGCTCTC AGAATGCAAG GCTAAACAGT CCTATGTGCC GGCGTTGACT 
GCAGACTCTC AGGGCCGCGT GGGCTGGCGC TGGATTCGGA TCGACACCGC TTGCGTCTGC 
ACGCTCCTCA GCCGAACAGG CCGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037316.2
CDS	207..836
Translation	MLPRHSCSLLLFLLLLPSVPMEPQPPSSTLPPFLAPEWDLLSPRVALSRGTPAGPPLLFLLEAGAYGEPAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKAESAGEGGPGVGGGGCRGVDRRHWLSECKAKQSYVPALTADSQGRVGWRWIRIDTACVCTLLSRTGRA

Target ORF information:

RefSeq Version	NM_013184.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurotrophin 4 (Ntf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013184.3

ORF Insert Sequence:

ATGCTCCCTC GCCACTCCTG CTCTCTCCTC CTTTTCCTTC TCCTCCTCCC CAGCGTCCCC 
ATGGAGCCCC AACCCCCCTC CTCCACCCTG CCCCCTTTTC TGGCTCCTGA GTGGGACCTC 
TTGTCTCCCC GAGTGGCCCT GTCAAGGGGC ACCCCTGCCG GGCCCCCTCT CCTCTTCCTG 
CTGGAGGCCG GGGCCTATGG GGAGCCGGCA GGGGCCCCAG CCAACCGCAG CCGGCGCGGG 
GTGAGTGAGA CGGCACCGGC AAGCCGCCGG GGTGAGCTGG CAGTGTGCGA TGCAGTGAGT 
GGCTGGGTGA CCGACCGGCG GACAGCTGTG GACTTGCGTG GGCGCGAGGT GGAGGTGCTG 
GGCGAGGTGC CTGCGGCAGG TGGCAGTCCC CTGCGTCAGT ACTTCTTCGA GACGCGCTGC 
AAGGCCGAAA GCGCTGGGGA AGGTGGCCCA GGTGTGGGCG GAGGGGGCTG TCGCGGCGTG 
GATCGGAGGC ACTGGCTCTC AGAATGCAAG GCTAAACAGT CCTATGTGCC GGCGTTGACT 
GCAGACTCTC AGGGCCGCGT GGGCTGGCGC TGGATTCGGA TCGACACCGC TTGCGTCTGC 
ACGCTCCTCA GCCGAACAGG CCGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BDNF and NT4 play interchangeable roles in gustatory development.
Developmental biology386(2)308-20(2014 Feb)
Huang T,Krimm RF

Increased expression of neurotrophin 4 following focal cerebral ischemia in adult rat brain with treadmill exercise.
PloS one8(3)e52461(2013)
Chung JY,Kim MW,Bang MS,Kim M

Effect of neurotrophin-4 on endoplasmic reticulum stress-related neuronal apoptosis in diabetic and high glucose exposed rat retinas.
Neuroscience letters501(2)102-6(2011 Aug)
Oshitari T,Yoshida-Hata N,Yamamoto S

Chronic ecstasy use increases neurotrophin-4 gene expression and protein levels in the rat brain.
Pharmacological reports : PR62(6)998-1004(2010 Nov-Dec)
Hatami H,Hossainpour-Faizi MA,Azarfarin M,Azarfam P

Deficits in LTP and recognition memory in the genetically hypertensive rat are associated with decreased expression of neurotrophic factors and their receptors in the dentate gyrus.
Behavioural brain research197(2)371-7(2009 Feb)
Hennigan A,Callaghan CK,Kealy J,Rouine J,Kelly AM