Col11a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col11a1
Entrez Gene ID	25654
Full Name	collagen type XI alpha 1 chain
General protein information	Preferred Names collagen type XI alpha 1 chain Names collagen alpha-1(XI) chain collagen type a1(XI)6A-7-8 collagen type a1(XI)6B-7 collagen, type XI, alpha 1 procollagen type XI alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q41
Summary	subunit of heterotrimeric collagen XI molecule which is expressed in developing cartilage and plays a role in regulation of collagen fibril diameter size; alternatively spliced as six different isoforms; may be a substrate for BMP-1 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233210.3	XP_006233272.1	collagen alpha-1(XI) chain isoform X2
XM_017590650.1	XP_017446139.1	collagen alpha-1(XI) chain isoform X1
XM_017590651.1	XP_017446140.1	collagen alpha-1(XI) chain isoform X3
NM_013117.1	NP_037249.1	collagen alpha-1(XI) chain precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL11A1	NP_001159981.1
Mus musculus (house mouse)	Col11a1	NP_031755.2
Danio rerio (zebrafish)	col11a1a	NP_001077313.1
Xenopus tropicalis (tropical clawed frog)	col5a3	XP_004913770.1
Rattus norvegicus (Norway rat)	Col11a1	NP_037249.1
Gallus gallus (chicken)	COL11A1	XP_001231624.2
Pan troglodytes (chimpanzee)	COL11A1	XP_001139974.1
	LOC712753	XP_002808276.1
Canis lupus familiaris (dog)	COL11A1	XP_005621909.1
Homo sapiens (human)	COL11A1	NP_542196.2

Gene Ontology
Bibliography

Related articles in PubMed

Changes in type II procollagen isoform expression during chondrogenesis by disruption of an alternative 5' splice site within Col2a1 exon 2.
Hering TM, Wirthlin L, Ravindran S, McAlinden A
Matrix biology : journal of the International Society for Matrix Biology3651-63(2014 Jun)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Brafman DA, Phung C, Kumar N, Willert K
Cell death and differentiation20(3)369-81(2013 Mar)

Regulation of collagen fibril nucleation and initial fibril assembly involves coordinate interactions with collagens V and XI in developing tendon.
Wenstrup RJ, Smith SM, Florer JB, Zhang G, Beason DP, Seegmiller RE, Soslowsky LJ, Birk DE
The Journal of biological chemistry286(23)20455-65(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two additional binding sites located within the noncollagenous amino-terminal domain may play a role in the function of collagen type XI
Title: Isoform-specific heparan sulfate binding within the amino-terminal noncollagenous domain of collagen alpha1(XI).

Bone morphogenetic protein 1 generates alternatively spliced isoforms of the collagen type XI a1 chain.
Title: BMP-1-mediated proteolytic processing of alternatively spliced isoforms of collagen type XI.

The following Col11a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col11a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02042	NM_013117.1 Latest version!	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa25206	XM_006233210.3 Latest version!	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa59635	XM_017590650.1 Latest version!	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa59636	XM_017590651.1 Latest version!	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col11a1 ( NM_013117.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037249.1 Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), mRNA.

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CloneID	ORa02042
Clone ID Related Accession (Same CDS sequence)	NM_013117.1
Accession Version	NM_013117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XI) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07012970.1. On or before Sep 1, 2012 this sequence version replaced XM_002726064.2, XM_002729104.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGTATGCA 
CCTGAGGATA TAATCGAGTA TGACTATGAA TATGGGGAGA CAGACTATAA AGAGGCTGAG 
AGTGTAACAG AGATGCCCAC TGTCACTGAA GAAACAGTAG CACAAACTGA GGCAAATATT 
GTGGATGACT TTCAAGACTA TAACTATGGA ACGATGGAAA CTTACCAGAC TGAGTCTCCT 
CGGCGGGTAT CAGGATCAAA TGAGCCAAAT CCAGTTGAAG AAGGTTTTAC TGAAGAATAT 
CTAACCGGAG AGGATTATGA TGTCCAGAGA AACATTTCCG AGGATATTCT GTATGGAAAC 
AAAGGAATAG ACGGCAGGGA TTCTGATCTG CTGGTAGATG GAGATTTAGG TGAATATGAT 
TTTTATGAAT ACAAAGAATA TGAAGAAAGG ACTACGACCT CCCCTAATGA AGAATTTGGC 
CCAGGTGTCC CAGCAGAAAC TGATTTCACA GAAACAAGCA TCAATGGCCA TGGCGCATAT 
GGAGAGAAAG GGCAGAAGGG GGAACCTGCT GTAGTTGAAC CCGGAATGCT TGTTGAAGGA 
CCACCTGGGC CAGCTGGACC AGCGGGTCTT ATGGGTCCTC CAGGACTACA AGGTCCTTCT 
GGGCTCCCCG GCGACCCTGG TGATAGGGGC CCACCAGGAC GCCCTGGCTT ACCAGGGGCT 
GATGGCTTAC CCGGTCCTCC AGGAACCATG TTAATGTTAC CATTCCGCTA TGGGGGTGAC 
GGCTCCAAAG GACCAACCAT CTCTGCGCAG GAAGCCCAGG CTCAGGCGAT TCTTCAGCAG 
GCTCGGATTG CTCTGAGAGG CCCTCCTGGC CCAATGGGTC TTACCGGAAG ACCAGGTCCT 
GTGGGGGGTC CTGGTTCAGC TGGAGCCAAA GGTGAGAGTG GGGATCCAGG TCCTCAGGGT 
CCTCGAGGTG TCCAAGGTCC TCCTGGTCCA ACCGGAAAAC CTGGAAAGAG GGGCCGTCCA 
GGTGCCGATG GAGGAAGAGG CATGCCAGGA GAACCTGGGT CTAAGGGAGA CCGAGGGTTT 
GATGGACTTC CAGGTCTGCC AGGTGACAAA GGTCACAGGG GGGAAAGAGG TCCACAAGGT 
CCACCTGGCC TCCCCGGCGA TGATGGAATG AGGGGAGAAG ATGGAGAGAT CGGGCCCAGG 
GGTCTTCCAG GGGAAGCTGG TCCACGAGGC TTGCTGGGAC CAAGGGGCAC TCCAGGACCT 
CCTGGGCAGC CTGGTATTGG AGGTATAGAT GGCCCCCAAG GACCAAAAGG AAACATGGGT 
CCTCAAGGGG AGCCTGGACC ACCAGGTCAG CAAGGAAATC CAGGACCTCA AGGACTTCCT 
GGTCCACAAG GCCCAATTGG TCCTCCTGGA GAAAAAGGAC CACAAGGAAA ACCAGGGCTT 
GCAGGACTTC CTGGTGCCGA TGGACCTCCT GGTCATCCTG GGAAAGAAGG CCAGTCTGGA 
GAAAAGGGTG CCCTGGGTCC TCCTGGTCCT CAGGGACCTA TTGGCTATCC CGGTCCCCGT 
GGAGTAAAGG GAGCAGATGG TGTCAGAGGT CTCAAGGGCT CTAAAGGAGA AAAGGGTGAA 
GATGGCTTTC CAGGATTCAA AGGTGACATG GGTCTTAAAG GTGACAGAGG CGAAGTGGGT 
CAAGTTGGTC CAAGAGGAGA AGATGGCCCT GAAGGCCCCA AAGGCCGTGC AGGCCCAACT 
GGAGACCCAG GTCCCTCTGG CCAAGCAGGA GAGAAGGGAA AACTTGGGGT TCCAGGATTG 
CCAGGATATC CAGGAAGACA AGGTCCAAAG GGATCCACTG GATTTCCTGG ATTTCCAGGT 
GCCAACGGGG AGAAAGGTGC CCGGGGAATT GCTGGCAAAC CAGGCCCCCG AGGACAACGT 
GGTCCAACGG GTCCTCGAGG TTCCAGAGGC GCCAGAGGTC CCACAGGAAA ACCTGGGCCG 
AAGGGTACAT CAGGTGGTGA TGGCCCTCCT GGCCCACCAG GCGAAAGGGG CCCTCAAGGA 
CCTCAAGGTC CAGTTGGATT CCCTGGACCA AAAGGCCCCC CTGGCCCTGC TGGGAAAGAT 
GGGCTACCAG GACACCCTGG ACAACGTGGA GAGACTGGAT TTCAAGGAAA GACCGGTCCC 
CCTGGGCCAG GAGGTGTGGT AGGACCACAG GGACCAACTG GTGAGACTGG CCCGATAGGT 
GAACGTGGGC ATCCTGGCAC TCCTGGTCCT CCGGGCGAGC AAGGACTTCC TGGTGCTGCA 
GGGAAAGAAG GTGCAAAGGG TGATCCTGGT CCTCAAGGTA TCTCAGGGAA AGATGGACCA 
GCAGGAATCC GTGGTTTCCC AGGTGAAAGA GGCCTTCCTG GAGCCCAGGG CGCACCTGGG 
TTGAAAGGAG GAGAAGGTCC CCAAGGCCCG CAGGGTCCAA TTGGCTCTCC TGGAGAGCGA 
GGCTCTGCAG GCACAGCAGG CCCAATTGGT TTGCCAGGAC GTCCAGGCCC TCAGGGCCCT 
CCTGGTCCAG CTGGAGAAAA AGGTGCTCCG GGAGAAAAGG GTCCTCAGGG GCCTGCAGGT 
AGAGATGGGG TCCAAGGCCC TGTGGGTCTC CCAGGGCCTG CTGGTCCTGC TGGCTCTCCT 
GGAGAAGATG GAGACAAGGG TGAAATTGGT GAACCAGGCC AAAAGGGCAG CAAAGGTGAC 
AAAGGCGAAA ATGGTCCTCC TGGTCCCCCG GGTCTTCAAG GCCCTGTTGG TGCTCCTGGA 
ATTGCTGGAG GAGATGGTGA GGCAGGTCCC CGAGGACAGC AGGGAATGTT TGGACAAAAA 
GGTGATGAGG GTGCACGAGG TTTCCCAGGA CCTCCTGGGC CCATAGGTCT TCAGGGGTTG 
CCAGGCCCAC CAGGTGAAAA GGGCGAAAAC GGAGACGTTG GCCCTATGGG TCCACCAGGT 
CCTCCTGGCC CACGAGGCCC TCAAGGTCCC AATGGAGCTG ATGGACCACA AGGACCCCCA 
GGCTCTATTG GTTCAGTTGG TGGTGTGGGA GAAAAGGGTG AACCAGGAGA AGCAGGGAAC 
CCAGGACCCC CTGGAGAAGC CGGATCAGGT GGTCCCAAAG GAGAGAGAGG AGAGAAAGGA 
GAAGCTGGGC CCCCTGGTGC TGCGGGTCCT CCTGGTATTA AGGGGCCACC AGGTGATGAT 
GGACCCAAGG GGAATCCGGG ACCTGTTGGG TTTCCTGGAG ATCCTGGTCC ACCTGGAGAA 
CCTGGTCCTG CAGGGCAAGA TGGTGTTGGA GGTGACAAGG GTGAAGATGG AGATCCAGGA 
CAACCAGGTC CCCCTGGTCC ATCCGGTGAA GCTGGCCCTC CAGGTCCACC TGGAAAGAGA 
GGTCCGCCTG GAGCTTCCGG TTCCGAAGGA AGGCAAGGAG AGAAAGGTGC TAAGGGAGAA 
GCTGGAGCTG AAGGACCTCC TGGGAAAACT GGCCCGGTCG GACCTCAGGG TCCTTCTGGA 
AAGCCTGGTC CAGAAGGTCT TCGAGGCATC CCTGGTCCTG TGGGAGAACA AGGTCTCCCC 
GGTGCTGCTG GCCAAGATGG ACCCCCTGGT CCTCTGGGAC CTCCTGGTTT ACCTGGTCTC 
AAAGGTGATC CTGGCTCCAA GGGTGAAAAG GGACATCCTG GTTTAATTGG ATTGATTGGC 
CCTCCAGGAG AACAAGGGGA AAAGGGTGAC AGAGGACTTC CTGGGACTCA AGGTTCTCCA 
GGAGCCAAAG GTGATGGGGG CATCCCAGGT CCTGCTGGTC CCATAGGTCC TCCTGGTCCT 
CCAGGATTAC CAGGTCCTGC TGGTCCGAAG GGTAACAAAG GATCTTCTGG ACCTACTGGC 
CAGAAGGGTG ACAGTGGTAT GCCAGGACCT CCTGGGCCTC CAGGTCCTCC TGGAGAAGTC 
ATACAGCCAT TACCTATTTT GTCACCGAAG AAAACAAGAA GACACACTGA AAGCATCCAG 
GCCGACGCAG GTGATAATAT TCTTGACTAC TCAGATGGCA TGGAGGAAAT ATTTGGTTCC 
CTCAATTCTC TGAAACAAGA CATTGAACAC ATGAAGTTTC CCATGGGTAC ACAGACCAAC 
CCAGCACGAA CATGCAAAGA CCTGCAGCTC AGCCACCCCG ACTTCCCAGA TGGTGAATAT 
TGGATTGATC CTAACCAAGG CTGCTCAGGT GATTCCTTCA AAGTTTACTG TAATTTCACA 
GCTGGTGGTG AGACGTGCAT CTATCCAGAC AAAAAATCTG AGGGAGTAAG ACTTTCATCT 
TGGCCGAAGG AGAAACCAGG AAGTTGGTAC AGTGAATTTA AGAGAGGAAA ACTGCTTTCC 
TATTTAGATG TGGAAGGCAA TTCCATCAAT ATGGTACAAA TGACATTCCT GAAACTCCTG 
ACTGCCTCTG CCAGACAAAA CTTCACCTAC AATTGCCACC AGTCAACTGC TTGGTATGAC 
GTCTTATCAG GAAGTTATGA CAAAGCACTT CGGTTTCTGG GATCTAATGA TGAGGAAATG 
TCCTACGAGA ACAACCCACA CATCAAAGCT TTGTATGACG GCTGTGCGTC CCGAAAAGGC 
TATGAAAAGA CAGTGATTGA GATCAATACT CCGAAAATTG ATCAAGTACC CATCATTGAT 
GTAATGATCA ATGATTTTGG TGATCAGAAT CAGAAGTTTG GATTTGAAGT TGGTCCAGCT 
TGCTTTCTTG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037249.1
CDS	353..5767
Translation	MEPWSRWKTKRWIWDLTISTLVLTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTSRKNSKDPDIAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFEDHTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRILETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEYAPEDIIEYDYEYGETDYKEAESVTEMPTVTEETVAQTEANIVDDFQDYNYGTMETYQTESPRRVSGSNEPNPVEEGFTEEYLTGEDYDVQRNISEDILYGNKGIDGRDSDLLVDGDLGEYDFYEYKEYEERTTTSPNEEFGPGVPAETDFTETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGTPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGPQGPIGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEAGPRGQQGMFGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGGVGEKGEPGEAGNPGPPGEAGSGGPKGERGEKGEAGPPGAAGPPGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEVIQPLPILSPKKTRRHTESIQADAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRLSSWPKEKPGSWYSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSTAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKALYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	NM_013117.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013117.1

ORF Insert Sequence:

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGTATGCA 
CCTGAGGATA TAATCGAGTA TGACTATGAA TATGGGGAGA CAGACTATAA AGAGGCTGAG 
AGTGTAACAG AGATGCCCAC TGTCACTGAA GAAACAGTAG CACAAACTGA GGCAAATATT 
GTGGATGACT TTCAAGACTA TAACTATGGA ACGATGGAAA CTTACCAGAC TGAGTCTCCT 
CGGCGGGTAT CAGGATCAAA TGAGCCAAAT CCAGTTGAAG AAGGTTTTAC TGAAGAATAT 
CTAACCGGAG AGGATTATGA TGTCCAGAGA AACATTTCCG AGGATATTCT GTATGGAAAC 
AAAGGAATAG ACGGCAGGGA TTCTGATCTG CTGGTAGATG GAGATTTAGG TGAATATGAT 
TTTTATGAAT ACAAAGAATA TGAAGAAAGG ACTACGACCT CCCCTAATGA AGAATTTGGC 
CCAGGTGTCC CAGCAGAAAC TGATTTCACA GAAACAAGCA TCAATGGCCA TGGCGCATAT 
GGAGAGAAAG GGCAGAAGGG GGAACCTGCT GTAGTTGAAC CCGGAATGCT TGTTGAAGGA 
CCACCTGGGC CAGCTGGACC AGCGGGTCTT ATGGGTCCTC CAGGACTACA AGGTCCTTCT 
GGGCTCCCCG GCGACCCTGG TGATAGGGGC CCACCAGGAC GCCCTGGCTT ACCAGGGGCT 
GATGGCTTAC CCGGTCCTCC AGGAACCATG TTAATGTTAC CATTCCGCTA TGGGGGTGAC 
GGCTCCAAAG GACCAACCAT CTCTGCGCAG GAAGCCCAGG CTCAGGCGAT TCTTCAGCAG 
GCTCGGATTG CTCTGAGAGG CCCTCCTGGC CCAATGGGTC TTACCGGAAG ACCAGGTCCT 
GTGGGGGGTC CTGGTTCAGC TGGAGCCAAA GGTGAGAGTG GGGATCCAGG TCCTCAGGGT 
CCTCGAGGTG TCCAAGGTCC TCCTGGTCCA ACCGGAAAAC CTGGAAAGAG GGGCCGTCCA 
GGTGCCGATG GAGGAAGAGG CATGCCAGGA GAACCTGGGT CTAAGGGAGA CCGAGGGTTT 
GATGGACTTC CAGGTCTGCC AGGTGACAAA GGTCACAGGG GGGAAAGAGG TCCACAAGGT 
CCACCTGGCC TCCCCGGCGA TGATGGAATG AGGGGAGAAG ATGGAGAGAT CGGGCCCAGG 
GGTCTTCCAG GGGAAGCTGG TCCACGAGGC TTGCTGGGAC CAAGGGGCAC TCCAGGACCT 
CCTGGGCAGC CTGGTATTGG AGGTATAGAT GGCCCCCAAG GACCAAAAGG AAACATGGGT 
CCTCAAGGGG AGCCTGGACC ACCAGGTCAG CAAGGAAATC CAGGACCTCA AGGACTTCCT 
GGTCCACAAG GCCCAATTGG TCCTCCTGGA GAAAAAGGAC CACAAGGAAA ACCAGGGCTT 
GCAGGACTTC CTGGTGCCGA TGGACCTCCT GGTCATCCTG GGAAAGAAGG CCAGTCTGGA 
GAAAAGGGTG CCCTGGGTCC TCCTGGTCCT CAGGGACCTA TTGGCTATCC CGGTCCCCGT 
GGAGTAAAGG GAGCAGATGG TGTCAGAGGT CTCAAGGGCT CTAAAGGAGA AAAGGGTGAA 
GATGGCTTTC CAGGATTCAA AGGTGACATG GGTCTTAAAG GTGACAGAGG CGAAGTGGGT 
CAAGTTGGTC CAAGAGGAGA AGATGGCCCT GAAGGCCCCA AAGGCCGTGC AGGCCCAACT 
GGAGACCCAG GTCCCTCTGG CCAAGCAGGA GAGAAGGGAA AACTTGGGGT TCCAGGATTG 
CCAGGATATC CAGGAAGACA AGGTCCAAAG GGATCCACTG GATTTCCTGG ATTTCCAGGT 
GCCAACGGGG AGAAAGGTGC CCGGGGAATT GCTGGCAAAC CAGGCCCCCG AGGACAACGT 
GGTCCAACGG GTCCTCGAGG TTCCAGAGGC GCCAGAGGTC CCACAGGAAA ACCTGGGCCG 
AAGGGTACAT CAGGTGGTGA TGGCCCTCCT GGCCCACCAG GCGAAAGGGG CCCTCAAGGA 
CCTCAAGGTC CAGTTGGATT CCCTGGACCA AAAGGCCCCC CTGGCCCTGC TGGGAAAGAT 
GGGCTACCAG GACACCCTGG ACAACGTGGA GAGACTGGAT TTCAAGGAAA GACCGGTCCC 
CCTGGGCCAG GAGGTGTGGT AGGACCACAG GGACCAACTG GTGAGACTGG CCCGATAGGT 
GAACGTGGGC ATCCTGGCAC TCCTGGTCCT CCGGGCGAGC AAGGACTTCC TGGTGCTGCA 
GGGAAAGAAG GTGCAAAGGG TGATCCTGGT CCTCAAGGTA TCTCAGGGAA AGATGGACCA 
GCAGGAATCC GTGGTTTCCC AGGTGAAAGA GGCCTTCCTG GAGCCCAGGG CGCACCTGGG 
TTGAAAGGAG GAGAAGGTCC CCAAGGCCCG CAGGGTCCAA TTGGCTCTCC TGGAGAGCGA 
GGCTCTGCAG GCACAGCAGG CCCAATTGGT TTGCCAGGAC GTCCAGGCCC TCAGGGCCCT 
CCTGGTCCAG CTGGAGAAAA AGGTGCTCCG GGAGAAAAGG GTCCTCAGGG GCCTGCAGGT 
AGAGATGGGG TCCAAGGCCC TGTGGGTCTC CCAGGGCCTG CTGGTCCTGC TGGCTCTCCT 
GGAGAAGATG GAGACAAGGG TGAAATTGGT GAACCAGGCC AAAAGGGCAG CAAAGGTGAC 
AAAGGCGAAA ATGGTCCTCC TGGTCCCCCG GGTCTTCAAG GCCCTGTTGG TGCTCCTGGA 
ATTGCTGGAG GAGATGGTGA GGCAGGTCCC CGAGGACAGC AGGGAATGTT TGGACAAAAA 
GGTGATGAGG GTGCACGAGG TTTCCCAGGA CCTCCTGGGC CCATAGGTCT TCAGGGGTTG 
CCAGGCCCAC CAGGTGAAAA GGGCGAAAAC GGAGACGTTG GCCCTATGGG TCCACCAGGT 
CCTCCTGGCC CACGAGGCCC TCAAGGTCCC AATGGAGCTG ATGGACCACA AGGACCCCCA 
GGCTCTATTG GTTCAGTTGG TGGTGTGGGA GAAAAGGGTG AACCAGGAGA AGCAGGGAAC 
CCAGGACCCC CTGGAGAAGC CGGATCAGGT GGTCCCAAAG GAGAGAGAGG AGAGAAAGGA 
GAAGCTGGGC CCCCTGGTGC TGCGGGTCCT CCTGGTATTA AGGGGCCACC AGGTGATGAT 
GGACCCAAGG GGAATCCGGG ACCTGTTGGG TTTCCTGGAG ATCCTGGTCC ACCTGGAGAA 
CCTGGTCCTG CAGGGCAAGA TGGTGTTGGA GGTGACAAGG GTGAAGATGG AGATCCAGGA 
CAACCAGGTC CCCCTGGTCC ATCCGGTGAA GCTGGCCCTC CAGGTCCACC TGGAAAGAGA 
GGTCCGCCTG GAGCTTCCGG TTCCGAAGGA AGGCAAGGAG AGAAAGGTGC TAAGGGAGAA 
GCTGGAGCTG AAGGACCTCC TGGGAAAACT GGCCCGGTCG GACCTCAGGG TCCTTCTGGA 
AAGCCTGGTC CAGAAGGTCT TCGAGGCATC CCTGGTCCTG TGGGAGAACA AGGTCTCCCC 
GGTGCTGCTG GCCAAGATGG ACCCCCTGGT CCTCTGGGAC CTCCTGGTTT ACCTGGTCTC 
AAAGGTGATC CTGGCTCCAA GGGTGAAAAG GGACATCCTG GTTTAATTGG ATTGATTGGC 
CCTCCAGGAG AACAAGGGGA AAAGGGTGAC AGAGGACTTC CTGGGACTCA AGGTTCTCCA 
GGAGCCAAAG GTGATGGGGG CATCCCAGGT CCTGCTGGTC CCATAGGTCC TCCTGGTCCT 
CCAGGATTAC CAGGTCCTGC TGGTCCGAAG GGTAACAAAG GATCTTCTGG ACCTACTGGC 
CAGAAGGGTG ACAGTGGTAT GCCAGGACCT CCTGGGCCTC CAGGTCCTCC TGGAGAAGTC 
ATACAGCCAT TACCTATTTT GTCACCGAAG AAAACAAGAA GACACACTGA AAGCATCCAG 
GCCGACGCAG GTGATAATAT TCTTGACTAC TCAGATGGCA TGGAGGAAAT ATTTGGTTCC 
CTCAATTCTC TGAAACAAGA CATTGAACAC ATGAAGTTTC CCATGGGTAC ACAGACCAAC 
CCAGCACGAA CATGCAAAGA CCTGCAGCTC AGCCACCCCG ACTTCCCAGA TGGTGAATAT 
TGGATTGATC CTAACCAAGG CTGCTCAGGT GATTCCTTCA AAGTTTACTG TAATTTCACA 
GCTGGTGGTG AGACGTGCAT CTATCCAGAC AAAAAATCTG AGGGAGTAAG ACTTTCATCT 
TGGCCGAAGG AGAAACCAGG AAGTTGGTAC AGTGAATTTA AGAGAGGAAA ACTGCTTTCC 
TATTTAGATG TGGAAGGCAA TTCCATCAAT ATGGTACAAA TGACATTCCT GAAACTCCTG 
ACTGCCTCTG CCAGACAAAA CTTCACCTAC AATTGCCACC AGTCAACTGC TTGGTATGAC 
GTCTTATCAG GAAGTTATGA CAAAGCACTT CGGTTTCTGG GATCTAATGA TGAGGAAATG 
TCCTACGAGA ACAACCCACA CATCAAAGCT TTGTATGACG GCTGTGCGTC CCGAAAAGGC 
TATGAAAAGA CAGTGATTGA GATCAATACT CCGAAAATTG ATCAAGTACC CATCATTGAT 
GTAATGATCA ATGATTTTGG TGATCAGAAT CAGAAGTTTG GATTTGAAGT TGGTCCAGCT 
TGCTTTCTTG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col11a1 ( XM_006233210.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233272.1 Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X2, mRNA.

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CloneID	ORa25206
Clone ID Related Accession (Same CDS sequence)	XM_006233210.3
Accession Version	XM_006233210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGAAAAAG 
AAATCCAATT ACACAAAGAA GAAGAGGACA TTGGCCACTA ACTCAAAGAA AAAATCTAAA 
ATGTCCACAA CCCCCAAATC TGAAAAGTTT GCATCCAAGA AGAAGAAACG TAACCAAGCC 
ACAGCCAAAG CCAAACTAGG GGTCCAGGCA AATATTGTGG ATGACTTTCA AGACTATAAC 
TATGGAACGA TGGAAACTTA CCAGACTGAG TCTCCTCGGC GGGTATCAGG ATCAAATGAG 
CCAAATCCAG TTGAAGAAGG TTTTACTGAA GAATATCTAA CCGGAGAGGA TTATGATGTC 
CAGAGAAACA TTTCCGAGGA TATTCTGTAT GGAAACAAAG GAATAGACGG CAGGGATTCT 
GATCTGCTGG TAGATGGAGA TTTAGGTGAA TATGATTTTT ATGAATACAA AGAATATGAA 
GAAAGGACTA CGACCTCCCC TAATGAAGAA TTTGGCCCAG GTGTCCCAGC AGAAACTGAT 
TTCACAGAAA CAAGCATCAA TGGCCATGGC GCATATGGAG AGAAAGGGCA GAAGGGGGAA 
CCTGCTGTAG TTGAACCCGG AATGCTTGTT GAAGGACCAC CTGGGCCAGC TGGACCAGCG 
GGTCTTATGG GTCCTCCAGG ACTACAAGGT CCTTCTGGGC TCCCCGGCGA CCCTGGTGAT 
AGGGGCCCAC CAGGACGCCC TGGCTTACCA GGGGCTGATG GCTTACCCGG TCCTCCAGGA 
ACCATGTTAA TGTTACCATT CCGCTATGGG GGTGACGGCT CCAAAGGACC AACCATCTCT 
GCGCAGGAAG CCCAGGCTCA GGCGATTCTT CAGCAGGCTC GGATTGCTCT GAGAGGCCCT 
CCTGGCCCAA TGGGTCTTAC CGGAAGACCA GGTCCTGTGG GGGGTCCTGG TTCAGCTGGA 
GCCAAAGGTG AGAGTGGGGA TCCAGGTCCT CAGGGTCCTC GAGGTGTCCA AGGTCCTCCT 
GGTCCAACCG GAAAACCTGG AAAGAGGGGC CGTCCAGGTG CCGATGGAGG AAGAGGCATG 
CCAGGAGAAC CTGGGTCTAA GGGAGACCGA GGGTTTGATG GACTTCCAGG TCTGCCAGGT 
GACAAAGGTC ACAGGGGGGA AAGAGGTCCA CAAGGTCCAC CTGGCCTCCC CGGCGATGAT 
GGAATGAGGG GAGAAGATGG AGAGATCGGG CCCAGGGGTC TTCCAGGGGA AGCTGGTCCA 
CGAGGCTTGC TGGGACCAAG GGGCACTCCA GGACCTCCTG GGCAGCCTGG TATTGGAGGT 
ATAGATGGCC CCCAAGGACC AAAAGGAAAC ATGGGTCCTC AAGGGGAGCC TGGACCACCA 
GGTCAGCAAG GAAATCCAGG ACCTCAAGGA CTTCCTGGTC CACAAGGCCC AATTGGTCCT 
CCTGGAGAAA AAGGACCACA AGGAAAACCA GGGCTTGCAG GACTTCCTGG TGCCGATGGA 
CCTCCTGGTC ATCCTGGGAA AGAAGGCCAG TCTGGAGAAA AGGGTGCCCT GGGTCCTCCT 
GGTCCTCAGG GACCTATTGG CTATCCCGGT CCCCGTGGAG TAAAGGGAGC AGATGGTGTC 
AGAGGTCTCA AGGGCTCTAA AGGAGAAAAG GGTGAAGATG GCTTTCCAGG ATTCAAAGGT 
GACATGGGTC TTAAAGGTGA CAGAGGCGAA GTGGGTCAAG TTGGTCCAAG AGGAGAAGAT 
GGCCCTGAAG GCCCCAAAGG CCGTGCAGGC CCAACTGGAG ACCCAGGTCC CTCTGGCCAA 
GCAGGAGAGA AGGGAAAACT TGGGGTTCCA GGATTGCCAG GATATCCAGG AAGACAAGGT 
CCAAAGGGAT CCACTGGATT TCCTGGATTT CCAGGTGCCA ACGGGGAGAA AGGTGCCCGG 
GGAATTGCTG GCAAACCAGG CCCCCGAGGA CAACGTGGTC CAACGGGTCC TCGAGGTTCC 
AGAGGCGCCA GAGGTCCCAC AGGAAAACCT GGGCCGAAGG GTACATCAGG TGGTGATGGC 
CCTCCTGGCC CACCAGGCGA AAGGGGCCCT CAAGGACCTC AAGGTCCAGT TGGATTCCCT 
GGACCAAAAG GCCCCCCTGG CCCTGCTGGG AAAGATGGGC TACCAGGACA CCCTGGACAA 
CGTGGAGAGA CTGGATTTCA AGGAAAGACC GGTCCCCCTG GGCCAGGAGG TGTGGTAGGA 
CCACAGGGAC CAACTGGTGA GACTGGCCCG ATAGGTGAAC GTGGGCATCC TGGCACTCCT 
GGTCCTCCGG GCGAGCAAGG ACTTCCTGGT GCTGCAGGGA AAGAAGGTGC AAAGGGTGAT 
CCTGGTCCTC AAGGTATCTC AGGGAAAGAT GGACCAGCAG GAATCCGTGG TTTCCCAGGT 
GAAAGAGGCC TTCCTGGAGC CCAGGGCGCA CCTGGGTTGA AAGGAGGAGA AGGTCCCCAA 
GGCCCGCAGG GTCCAATTGG CTCTCCTGGA GAGCGAGGCT CTGCAGGCAC AGCAGGCCCA 
ATTGGTTTGC CAGGACGTCC AGGCCCTCAG GGCCCTCCTG GTCCAGCTGG AGAAAAAGGT 
GCTCCGGGAG AAAAGGGTCC TCAGGGGCCT GCAGGTAGAG ATGGGGTCCA AGGCCCTGTG 
GGTCTCCCAG GGCCTGCTGG TCCTGCTGGC TCTCCTGGAG AAGATGGAGA CAAGGGTGAA 
ATTGGTGAAC CAGGCCAAAA GGGCAGCAAA GGTGACAAAG GCGAAAATGG TCCTCCTGGT 
CCCCCGGGTC TTCAAGGCCC TGTTGGTGCT CCTGGAATTG CTGGAGGAGA TGGTGAGGCA 
GGTCCCCGAG GACAGCAGGG AATGTTTGGA CAAAAAGGTG ATGAGGGTGC ACGAGGTTTC 
CCAGGACCTC CTGGGCCCAT AGGTCTTCAG GGGTTGCCAG GCCCACCAGG TGAAAAGGGC 
GAAAACGGAG ACGTTGGCCC TATGGGTCCA CCAGGTCCTC CTGGCCCACG AGGCCCTCAA 
GGTCCCAATG GAGCTGATGG ACCACAAGGA CCCCCAGGCT CTATTGGTTC AGTTGGTGGT 
GTGGGAGAAA AGGGTGAACC AGGAGAAGCA GGGAACCCAG GACCCCCTGG AGAAGCCGGA 
TCAGGTGGTC CCAAAGGAGA GAGAGGAGAG AAAGGAGAAG CTGGGCCCCC TGGTGCTGCG 
GGTCCTCCTG GTATTAAGGG GCCACCAGGT GATGATGGAC CCAAGGGGAA TCCGGGACCT 
GTTGGGTTTC CTGGAGATCC TGGTCCACCT GGAGAACCTG GTCCTGCAGG GCAAGATGGT 
GTTGGAGGTG ACAAGGGTGA AGATGGAGAT CCAGGACAAC CAGGTCCCCC TGGTCCATCC 
GGTGAAGCTG GCCCTCCAGG TCCACCTGGA AAGAGAGGTC CGCCTGGAGC TTCCGGTTCC 
GAAGGAAGGC AAGGAGAGAA AGGTGCTAAG GGAGAAGCTG GAGCTGAAGG ACCTCCTGGG 
AAAACTGGCC CGGTCGGACC TCAGGGTCCT TCTGGAAAGC CTGGTCCAGA AGGTCTTCGA 
GGCATCCCTG GTCCTGTGGG AGAACAAGGT CTCCCCGGTG CTGCTGGCCA AGATGGACCC 
CCTGGTCCTC TGGGACCTCC TGGTTTACCT GGTCTCAAAG GTGATCCTGG CTCCAAGGGT 
GAAAAGGGAC ATCCTGGTTT AATTGGATTG ATTGGCCCTC CAGGAGAACA AGGGGAAAAG 
GGTGACAGAG GACTTCCTGG GACTCAAGGT TCTCCAGGAG CCAAAGGTGA TGGGGGCATC 
CCAGGTCCTG CTGGTCCCAT AGGTCCTCCT GGTCCTCCAG GATTACCAGG TCCTGCTGGT 
CCGAAGGGTA ACAAAGGATC TTCTGGACCT ACTGGCCAGA AGGGTGACAG TGGTATGCCA 
GGACCTCCTG GGCCTCCAGG TCCTCCTGGA GAAGTCATAC AGCCATTACC TATTTTGTCA 
CCGAAGAAAA CAAGAAGACA CACTGAAAGC ATCCAGGCCG ACGCAGGTGA TAATATTCTT 
GACTACTCAG ATGGCATGGA GGAAATATTT GGTTCCCTCA ATTCTCTGAA ACAAGACATT 
GAACACATGA AGTTTCCCAT GGGTACACAG ACCAACCCAG CACGAACATG CAAAGACCTG 
CAGCTCAGCC ACCCCGACTT CCCAGATGGT GAATATTGGA TTGATCCTAA CCAAGGCTGC 
TCAGGTGATT CCTTCAAAGT TTACTGTAAT TTCACAGCTG GTGGTGAGAC GTGCATCTAT 
CCAGACAAAA AATCTGAGGG AGTAAGACTT TCATCTTGGC CGAAGGAGAA ACCAGGAAGT 
TGGTACAGTG AATTTAAGAG AGGAAAACTG CTTTCCTATT TAGATGTGGA AGGCAATTCC 
ATCAATATGG TACAAATGAC ATTCCTGAAA CTCCTGACTG CCTCTGCCAG ACAAAACTTC 
ACCTACAATT GCCACCAGTC AACTGCTTGG TATGACGTCT TATCAGGAAG TTATGACAAA 
GCACTTCGGT TTCTGGGATC TAATGATGAG GAAATGTCCT ACGAGAACAA CCCACACATC 
AAAGCTTTGT ATGACGGCTG TGCGTCCCGA AAAGGCTATG AAAAGACAGT GATTGAGATC 
AATACTCCGA AAATTGATCA AGTACCCATC ATTGATGTAA TGATCAATGA TTTTGGTGAT 
CAGAATCAGA AGTTTGGATT TGAAGTTGGT CCAGCTTGCT TTCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233272.1
CDS	358..5808
Translation	MEPWSRWKTKRWIWDLTISTLVLTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTSRKNSKDPDIAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFEDHTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRILETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEKKKSNYTKKKRTLATNSKKKSKMSTTPKSEKFASKKKKRNQATAKAKLGVQANIVDDFQDYNYGTMETYQTESPRRVSGSNEPNPVEEGFTEEYLTGEDYDVQRNISEDILYGNKGIDGRDSDLLVDGDLGEYDFYEYKEYEERTTTSPNEEFGPGVPAETDFTETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGTPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGPQGPIGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEAGPRGQQGMFGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGGVGEKGEPGEAGNPGPPGEAGSGGPKGERGEKGEAGPPGAAGPPGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEVIQPLPILSPKKTRRHTESIQADAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRLSSWPKEKPGSWYSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSTAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKALYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_006233210.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233210.3

ORF Insert Sequence:

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGAAAAAG 
AAATCCAATT ACACAAAGAA GAAGAGGACA TTGGCCACTA ACTCAAAGAA AAAATCTAAA 
ATGTCCACAA CCCCCAAATC TGAAAAGTTT GCATCCAAGA AGAAGAAACG TAACCAAGCC 
ACAGCCAAAG CCAAACTAGG GGTCCAGGCA AATATTGTGG ATGACTTTCA AGACTATAAC 
TATGGAACGA TGGAAACTTA CCAGACTGAG TCTCCTCGGC GGGTATCAGG ATCAAATGAG 
CCAAATCCAG TTGAAGAAGG TTTTACTGAA GAATATCTAA CCGGAGAGGA TTATGATGTC 
CAGAGAAACA TTTCCGAGGA TATTCTGTAT GGAAACAAAG GAATAGACGG CAGGGATTCT 
GATCTGCTGG TAGATGGAGA TTTAGGTGAA TATGATTTTT ATGAATACAA AGAATATGAA 
GAAAGGACTA CGACCTCCCC TAATGAAGAA TTTGGCCCAG GTGTCCCAGC AGAAACTGAT 
TTCACAGAAA CAAGCATCAA TGGCCATGGC GCATATGGAG AGAAAGGGCA GAAGGGGGAA 
CCTGCTGTAG TTGAACCCGG AATGCTTGTT GAAGGACCAC CTGGGCCAGC TGGACCAGCG 
GGTCTTATGG GTCCTCCAGG ACTACAAGGT CCTTCTGGGC TCCCCGGCGA CCCTGGTGAT 
AGGGGCCCAC CAGGACGCCC TGGCTTACCA GGGGCTGATG GCTTACCCGG TCCTCCAGGA 
ACCATGTTAA TGTTACCATT CCGCTATGGG GGTGACGGCT CCAAAGGACC AACCATCTCT 
GCGCAGGAAG CCCAGGCTCA GGCGATTCTT CAGCAGGCTC GGATTGCTCT GAGAGGCCCT 
CCTGGCCCAA TGGGTCTTAC CGGAAGACCA GGTCCTGTGG GGGGTCCTGG TTCAGCTGGA 
GCCAAAGGTG AGAGTGGGGA TCCAGGTCCT CAGGGTCCTC GAGGTGTCCA AGGTCCTCCT 
GGTCCAACCG GAAAACCTGG AAAGAGGGGC CGTCCAGGTG CCGATGGAGG AAGAGGCATG 
CCAGGAGAAC CTGGGTCTAA GGGAGACCGA GGGTTTGATG GACTTCCAGG TCTGCCAGGT 
GACAAAGGTC ACAGGGGGGA AAGAGGTCCA CAAGGTCCAC CTGGCCTCCC CGGCGATGAT 
GGAATGAGGG GAGAAGATGG AGAGATCGGG CCCAGGGGTC TTCCAGGGGA AGCTGGTCCA 
CGAGGCTTGC TGGGACCAAG GGGCACTCCA GGACCTCCTG GGCAGCCTGG TATTGGAGGT 
ATAGATGGCC CCCAAGGACC AAAAGGAAAC ATGGGTCCTC AAGGGGAGCC TGGACCACCA 
GGTCAGCAAG GAAATCCAGG ACCTCAAGGA CTTCCTGGTC CACAAGGCCC AATTGGTCCT 
CCTGGAGAAA AAGGACCACA AGGAAAACCA GGGCTTGCAG GACTTCCTGG TGCCGATGGA 
CCTCCTGGTC ATCCTGGGAA AGAAGGCCAG TCTGGAGAAA AGGGTGCCCT GGGTCCTCCT 
GGTCCTCAGG GACCTATTGG CTATCCCGGT CCCCGTGGAG TAAAGGGAGC AGATGGTGTC 
AGAGGTCTCA AGGGCTCTAA AGGAGAAAAG GGTGAAGATG GCTTTCCAGG ATTCAAAGGT 
GACATGGGTC TTAAAGGTGA CAGAGGCGAA GTGGGTCAAG TTGGTCCAAG AGGAGAAGAT 
GGCCCTGAAG GCCCCAAAGG CCGTGCAGGC CCAACTGGAG ACCCAGGTCC CTCTGGCCAA 
GCAGGAGAGA AGGGAAAACT TGGGGTTCCA GGATTGCCAG GATATCCAGG AAGACAAGGT 
CCAAAGGGAT CCACTGGATT TCCTGGATTT CCAGGTGCCA ACGGGGAGAA AGGTGCCCGG 
GGAATTGCTG GCAAACCAGG CCCCCGAGGA CAACGTGGTC CAACGGGTCC TCGAGGTTCC 
AGAGGCGCCA GAGGTCCCAC AGGAAAACCT GGGCCGAAGG GTACATCAGG TGGTGATGGC 
CCTCCTGGCC CACCAGGCGA AAGGGGCCCT CAAGGACCTC AAGGTCCAGT TGGATTCCCT 
GGACCAAAAG GCCCCCCTGG CCCTGCTGGG AAAGATGGGC TACCAGGACA CCCTGGACAA 
CGTGGAGAGA CTGGATTTCA AGGAAAGACC GGTCCCCCTG GGCCAGGAGG TGTGGTAGGA 
CCACAGGGAC CAACTGGTGA GACTGGCCCG ATAGGTGAAC GTGGGCATCC TGGCACTCCT 
GGTCCTCCGG GCGAGCAAGG ACTTCCTGGT GCTGCAGGGA AAGAAGGTGC AAAGGGTGAT 
CCTGGTCCTC AAGGTATCTC AGGGAAAGAT GGACCAGCAG GAATCCGTGG TTTCCCAGGT 
GAAAGAGGCC TTCCTGGAGC CCAGGGCGCA CCTGGGTTGA AAGGAGGAGA AGGTCCCCAA 
GGCCCGCAGG GTCCAATTGG CTCTCCTGGA GAGCGAGGCT CTGCAGGCAC AGCAGGCCCA 
ATTGGTTTGC CAGGACGTCC AGGCCCTCAG GGCCCTCCTG GTCCAGCTGG AGAAAAAGGT 
GCTCCGGGAG AAAAGGGTCC TCAGGGGCCT GCAGGTAGAG ATGGGGTCCA AGGCCCTGTG 
GGTCTCCCAG GGCCTGCTGG TCCTGCTGGC TCTCCTGGAG AAGATGGAGA CAAGGGTGAA 
ATTGGTGAAC CAGGCCAAAA GGGCAGCAAA GGTGACAAAG GCGAAAATGG TCCTCCTGGT 
CCCCCGGGTC TTCAAGGCCC TGTTGGTGCT CCTGGAATTG CTGGAGGAGA TGGTGAGGCA 
GGTCCCCGAG GACAGCAGGG AATGTTTGGA CAAAAAGGTG ATGAGGGTGC ACGAGGTTTC 
CCAGGACCTC CTGGGCCCAT AGGTCTTCAG GGGTTGCCAG GCCCACCAGG TGAAAAGGGC 
GAAAACGGAG ACGTTGGCCC TATGGGTCCA CCAGGTCCTC CTGGCCCACG AGGCCCTCAA 
GGTCCCAATG GAGCTGATGG ACCACAAGGA CCCCCAGGCT CTATTGGTTC AGTTGGTGGT 
GTGGGAGAAA AGGGTGAACC AGGAGAAGCA GGGAACCCAG GACCCCCTGG AGAAGCCGGA 
TCAGGTGGTC CCAAAGGAGA GAGAGGAGAG AAAGGAGAAG CTGGGCCCCC TGGTGCTGCG 
GGTCCTCCTG GTATTAAGGG GCCACCAGGT GATGATGGAC CCAAGGGGAA TCCGGGACCT 
GTTGGGTTTC CTGGAGATCC TGGTCCACCT GGAGAACCTG GTCCTGCAGG GCAAGATGGT 
GTTGGAGGTG ACAAGGGTGA AGATGGAGAT CCAGGACAAC CAGGTCCCCC TGGTCCATCC 
GGTGAAGCTG GCCCTCCAGG TCCACCTGGA AAGAGAGGTC CGCCTGGAGC TTCCGGTTCC 
GAAGGAAGGC AAGGAGAGAA AGGTGCTAAG GGAGAAGCTG GAGCTGAAGG ACCTCCTGGG 
AAAACTGGCC CGGTCGGACC TCAGGGTCCT TCTGGAAAGC CTGGTCCAGA AGGTCTTCGA 
GGCATCCCTG GTCCTGTGGG AGAACAAGGT CTCCCCGGTG CTGCTGGCCA AGATGGACCC 
CCTGGTCCTC TGGGACCTCC TGGTTTACCT GGTCTCAAAG GTGATCCTGG CTCCAAGGGT 
GAAAAGGGAC ATCCTGGTTT AATTGGATTG ATTGGCCCTC CAGGAGAACA AGGGGAAAAG 
GGTGACAGAG GACTTCCTGG GACTCAAGGT TCTCCAGGAG CCAAAGGTGA TGGGGGCATC 
CCAGGTCCTG CTGGTCCCAT AGGTCCTCCT GGTCCTCCAG GATTACCAGG TCCTGCTGGT 
CCGAAGGGTA ACAAAGGATC TTCTGGACCT ACTGGCCAGA AGGGTGACAG TGGTATGCCA 
GGACCTCCTG GGCCTCCAGG TCCTCCTGGA GAAGTCATAC AGCCATTACC TATTTTGTCA 
CCGAAGAAAA CAAGAAGACA CACTGAAAGC ATCCAGGCCG ACGCAGGTGA TAATATTCTT 
GACTACTCAG ATGGCATGGA GGAAATATTT GGTTCCCTCA ATTCTCTGAA ACAAGACATT 
GAACACATGA AGTTTCCCAT GGGTACACAG ACCAACCCAG CACGAACATG CAAAGACCTG 
CAGCTCAGCC ACCCCGACTT CCCAGATGGT GAATATTGGA TTGATCCTAA CCAAGGCTGC 
TCAGGTGATT CCTTCAAAGT TTACTGTAAT TTCACAGCTG GTGGTGAGAC GTGCATCTAT 
CCAGACAAAA AATCTGAGGG AGTAAGACTT TCATCTTGGC CGAAGGAGAA ACCAGGAAGT 
TGGTACAGTG AATTTAAGAG AGGAAAACTG CTTTCCTATT TAGATGTGGA AGGCAATTCC 
ATCAATATGG TACAAATGAC ATTCCTGAAA CTCCTGACTG CCTCTGCCAG ACAAAACTTC 
ACCTACAATT GCCACCAGTC AACTGCTTGG TATGACGTCT TATCAGGAAG TTATGACAAA 
GCACTTCGGT TTCTGGGATC TAATGATGAG GAAATGTCCT ACGAGAACAA CCCACACATC 
AAAGCTTTGT ATGACGGCTG TGCGTCCCGA AAAGGCTATG AAAAGACAGT GATTGAGATC 
AATACTCCGA AAATTGATCA AGTACCCATC ATTGATGTAA TGATCAATGA TTTTGGTGAT 
CAGAATCAGA AGTTTGGATT TGAAGTTGGT CCAGCTTGCT TTCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col11a1 ( XM_017590650.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446139.1 Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X1, mRNA.

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CloneID	ORa59635
Clone ID Related Accession (Same CDS sequence)	XM_017590650.1
Accession Version	XM_017590650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGTATGCA 
CCTGAGGATA TAATCGAGTA TGACTATGAA TATGGGGAGA CAGACTATAA AGAGGCTGAG 
AGTGTAACAG AGATGCCCAC TGTCACTGAA GAAACAGTAG CACAAACTGA GAAAAAGAAA 
TCCAATTACA CAAAGAAGAA GAGGACATTG GCCACTAACT CAAAGAAAAA ATCTAAAATG 
TCCACAACCC CCAAATCTGA AAAGTTTGCA TCCAAGAAGA AGAAACGTAA CCAAGCCACA 
GCCAAAGCCA AACTAGGGGT CCAGGCAAAT ATTGTGGATG ACTTTCAAGA CTATAACTAT 
GGAACGATGG AAACTTACCA GACTGAGTCT CCTCGGCGGG TATCAGGATC AAATGAGCCA 
AATCCAGTTG AAGAAGGTTT TACTGAAGAA TATCTAACCG GAGAGGATTA TGATGTCCAG 
AGAAACATTT CCGAGGATAT TCTGTATGGA AACAAAGGAA TAGACGGCAG GGATTCTGAT 
CTGCTGGTAG ATGGAGATTT AGGTGAATAT GATTTTTATG AATACAAAGA ATATGAAGAA 
AGGACTACGA CCTCCCCTAA TGAAGAATTT GGCCCAGGTG TCCCAGCAGA AACTGATTTC 
ACAGAAACAA GCATCAATGG CCATGGCGCA TATGGAGAGA AAGGGCAGAA GGGGGAACCT 
GCTGTAGTTG AACCCGGAAT GCTTGTTGAA GGACCACCTG GGCCAGCTGG ACCAGCGGGT 
CTTATGGGTC CTCCAGGACT ACAAGGTCCT TCTGGGCTCC CCGGCGACCC TGGTGATAGG 
GGCCCACCAG GACGCCCTGG CTTACCAGGG GCTGATGGCT TACCCGGTCC TCCAGGAACC 
ATGTTAATGT TACCATTCCG CTATGGGGGT GACGGCTCCA AAGGACCAAC CATCTCTGCG 
CAGGAAGCCC AGGCTCAGGC GATTCTTCAG CAGGCTCGGA TTGCTCTGAG AGGCCCTCCT 
GGCCCAATGG GTCTTACCGG AAGACCAGGT CCTGTGGGGG GTCCTGGTTC AGCTGGAGCC 
AAAGGTGAGA GTGGGGATCC AGGTCCTCAG GGTCCTCGAG GTGTCCAAGG TCCTCCTGGT 
CCAACCGGAA AACCTGGAAA GAGGGGCCGT CCAGGTGCCG ATGGAGGAAG AGGCATGCCA 
GGAGAACCTG GGTCTAAGGG AGACCGAGGG TTTGATGGAC TTCCAGGTCT GCCAGGTGAC 
AAAGGTCACA GGGGGGAAAG AGGTCCACAA GGTCCACCTG GCCTCCCCGG CGATGATGGA 
ATGAGGGGAG AAGATGGAGA GATCGGGCCC AGGGGTCTTC CAGGGGAAGC TGGTCCACGA 
GGCTTGCTGG GACCAAGGGG CACTCCAGGA CCTCCTGGGC AGCCTGGTAT TGGAGGTATA 
GATGGCCCCC AAGGACCAAA AGGAAACATG GGTCCTCAAG GGGAGCCTGG ACCACCAGGT 
CAGCAAGGAA ATCCAGGACC TCAAGGACTT CCTGGTCCAC AAGGCCCAAT TGGTCCTCCT 
GGAGAAAAAG GACCACAAGG AAAACCAGGG CTTGCAGGAC TTCCTGGTGC CGATGGACCT 
CCTGGTCATC CTGGGAAAGA AGGCCAGTCT GGAGAAAAGG GTGCCCTGGG TCCTCCTGGT 
CCTCAGGGAC CTATTGGCTA TCCCGGTCCC CGTGGAGTAA AGGGAGCAGA TGGTGTCAGA 
GGTCTCAAGG GCTCTAAAGG AGAAAAGGGT GAAGATGGCT TTCCAGGATT CAAAGGTGAC 
ATGGGTCTTA AAGGTGACAG AGGCGAAGTG GGTCAAGTTG GTCCAAGAGG AGAAGATGGC 
CCTGAAGGCC CCAAAGGCCG TGCAGGCCCA ACTGGAGACC CAGGTCCCTC TGGCCAAGCA 
GGAGAGAAGG GAAAACTTGG GGTTCCAGGA TTGCCAGGAT ATCCAGGAAG ACAAGGTCCA 
AAGGGATCCA CTGGATTTCC TGGATTTCCA GGTGCCAACG GGGAGAAAGG TGCCCGGGGA 
ATTGCTGGCA AACCAGGCCC CCGAGGACAA CGTGGTCCAA CGGGTCCTCG AGGTTCCAGA 
GGCGCCAGAG GTCCCACAGG AAAACCTGGG CCGAAGGGTA CATCAGGTGG TGATGGCCCT 
CCTGGCCCAC CAGGCGAAAG GGGCCCTCAA GGACCTCAAG GTCCAGTTGG ATTCCCTGGA 
CCAAAAGGCC CCCCTGGCCC TGCTGGGAAA GATGGGCTAC CAGGACACCC TGGACAACGT 
GGAGAGACTG GATTTCAAGG AAAGACCGGT CCCCCTGGGC CAGGAGGTGT GGTAGGACCA 
CAGGGACCAA CTGGTGAGAC TGGCCCGATA GGTGAACGTG GGCATCCTGG CACTCCTGGT 
CCTCCGGGCG AGCAAGGACT TCCTGGTGCT GCAGGGAAAG AAGGTGCAAA GGGTGATCCT 
GGTCCTCAAG GTATCTCAGG GAAAGATGGA CCAGCAGGAA TCCGTGGTTT CCCAGGTGAA 
AGAGGCCTTC CTGGAGCCCA GGGCGCACCT GGGTTGAAAG GAGGAGAAGG TCCCCAAGGC 
CCGCAGGGTC CAATTGGCTC TCCTGGAGAG CGAGGCTCTG CAGGCACAGC AGGCCCAATT 
GGTTTGCCAG GACGTCCAGG CCCTCAGGGC CCTCCTGGTC CAGCTGGAGA AAAAGGTGCT 
CCGGGAGAAA AGGGTCCTCA GGGGCCTGCA GGTAGAGATG GGGTCCAAGG CCCTGTGGGT 
CTCCCAGGGC CTGCTGGTCC TGCTGGCTCT CCTGGAGAAG ATGGAGACAA GGGTGAAATT 
GGTGAACCAG GCCAAAAGGG CAGCAAAGGT GACAAAGGCG AAAATGGTCC TCCTGGTCCC 
CCGGGTCTTC AAGGCCCTGT TGGTGCTCCT GGAATTGCTG GAGGAGATGG TGAGGCAGGT 
CCCCGAGGAC AGCAGGGAAT GTTTGGACAA AAAGGTGATG AGGGTGCACG AGGTTTCCCA 
GGACCTCCTG GGCCCATAGG TCTTCAGGGG TTGCCAGGCC CACCAGGTGA AAAGGGCGAA 
AACGGAGACG TTGGCCCTAT GGGTCCACCA GGTCCTCCTG GCCCACGAGG CCCTCAAGGT 
CCCAATGGAG CTGATGGACC ACAAGGACCC CCAGGCTCTA TTGGTTCAGT TGGTGGTGTG 
GGAGAAAAGG GTGAACCAGG AGAAGCAGGG AACCCAGGAC CCCCTGGAGA AGCCGGATCA 
GGTGGTCCCA AAGGAGAGAG AGGAGAGAAA GGAGAAGCTG GGCCCCCTGG TGCTGCGGGT 
CCTCCTGGTA TTAAGGGGCC ACCAGGTGAT GATGGACCCA AGGGGAATCC GGGACCTGTT 
GGGTTTCCTG GAGATCCTGG TCCACCTGGA GAACCTGGTC CTGCAGGGCA AGATGGTGTT 
GGAGGTGACA AGGGTGAAGA TGGAGATCCA GGACAACCAG GTCCCCCTGG TCCATCCGGT 
GAAGCTGGCC CTCCAGGTCC ACCTGGAAAG AGAGGTCCGC CTGGAGCTTC CGGTTCCGAA 
GGAAGGCAAG GAGAGAAAGG TGCTAAGGGA GAAGCTGGAG CTGAAGGACC TCCTGGGAAA 
ACTGGCCCGG TCGGACCTCA GGGTCCTTCT GGAAAGCCTG GTCCAGAAGG TCTTCGAGGC 
ATCCCTGGTC CTGTGGGAGA ACAAGGTCTC CCCGGTGCTG CTGGCCAAGA TGGACCCCCT 
GGTCCTCTGG GACCTCCTGG TTTACCTGGT CTCAAAGGTG ATCCTGGCTC CAAGGGTGAA 
AAGGGACATC CTGGTTTAAT TGGATTGATT GGCCCTCCAG GAGAACAAGG GGAAAAGGGT 
GACAGAGGAC TTCCTGGGAC TCAAGGTTCT CCAGGAGCCA AAGGTGATGG GGGCATCCCA 
GGTCCTGCTG GTCCCATAGG TCCTCCTGGT CCTCCAGGAT TACCAGGTCC TGCTGGTCCG 
AAGGGTAACA AAGGATCTTC TGGACCTACT GGCCAGAAGG GTGACAGTGG TATGCCAGGA 
CCTCCTGGGC CTCCAGGTCC TCCTGGAGAA GTCATACAGC CATTACCTAT TTTGTCACCG 
AAGAAAACAA GAAGACACAC TGAAAGCATC CAGGCCGACG CAGGTGATAA TATTCTTGAC 
TACTCAGATG GCATGGAGGA AATATTTGGT TCCCTCAATT CTCTGAAACA AGACATTGAA 
CACATGAAGT TTCCCATGGG TACACAGACC AACCCAGCAC GAACATGCAA AGACCTGCAG 
CTCAGCCACC CCGACTTCCC AGATGGTGAA TATTGGATTG ATCCTAACCA AGGCTGCTCA 
GGTGATTCCT TCAAAGTTTA CTGTAATTTC ACAGCTGGTG GTGAGACGTG CATCTATCCA 
GACAAAAAAT CTGAGGGAGT AAGACTTTCA TCTTGGCCGA AGGAGAAACC AGGAAGTTGG 
TACAGTGAAT TTAAGAGAGG AAAACTGCTT TCCTATTTAG ATGTGGAAGG CAATTCCATC 
AATATGGTAC AAATGACATT CCTGAAACTC CTGACTGCCT CTGCCAGACA AAACTTCACC 
TACAATTGCC ACCAGTCAAC TGCTTGGTAT GACGTCTTAT CAGGAAGTTA TGACAAAGCA 
CTTCGGTTTC TGGGATCTAA TGATGAGGAA ATGTCCTACG AGAACAACCC ACACATCAAA 
GCTTTGTATG ACGGCTGTGC GTCCCGAAAA GGCTATGAAA AGACAGTGAT TGAGATCAAT 
ACTCCGAAAA TTGATCAAGT ACCCATCATT GATGTAATGA TCAATGATTT TGGTGATCAG 
AATCAGAAGT TTGGATTTGA AGTTGGTCCA GCTTGCTTTC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446139.1
CDS	358..5925
Translation	MEPWSRWKTKRWIWDLTISTLVLTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTSRKNSKDPDIAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFEDHTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRILETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEYAPEDIIEYDYEYGETDYKEAESVTEMPTVTEETVAQTEKKKSNYTKKKRTLATNSKKKSKMSTTPKSEKFASKKKKRNQATAKAKLGVQANIVDDFQDYNYGTMETYQTESPRRVSGSNEPNPVEEGFTEEYLTGEDYDVQRNISEDILYGNKGIDGRDSDLLVDGDLGEYDFYEYKEYEERTTTSPNEEFGPGVPAETDFTETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGTPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGPQGPIGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEAGPRGQQGMFGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGGVGEKGEPGEAGNPGPPGEAGSGGPKGERGEKGEAGPPGAAGPPGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEVIQPLPILSPKKTRRHTESIQADAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRLSSWPKEKPGSWYSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSTAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKALYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_017590650.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590650.1

ORF Insert Sequence:

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGTATGCA 
CCTGAGGATA TAATCGAGTA TGACTATGAA TATGGGGAGA CAGACTATAA AGAGGCTGAG 
AGTGTAACAG AGATGCCCAC TGTCACTGAA GAAACAGTAG CACAAACTGA GAAAAAGAAA 
TCCAATTACA CAAAGAAGAA GAGGACATTG GCCACTAACT CAAAGAAAAA ATCTAAAATG 
TCCACAACCC CCAAATCTGA AAAGTTTGCA TCCAAGAAGA AGAAACGTAA CCAAGCCACA 
GCCAAAGCCA AACTAGGGGT CCAGGCAAAT ATTGTGGATG ACTTTCAAGA CTATAACTAT 
GGAACGATGG AAACTTACCA GACTGAGTCT CCTCGGCGGG TATCAGGATC AAATGAGCCA 
AATCCAGTTG AAGAAGGTTT TACTGAAGAA TATCTAACCG GAGAGGATTA TGATGTCCAG 
AGAAACATTT CCGAGGATAT TCTGTATGGA AACAAAGGAA TAGACGGCAG GGATTCTGAT 
CTGCTGGTAG ATGGAGATTT AGGTGAATAT GATTTTTATG AATACAAAGA ATATGAAGAA 
AGGACTACGA CCTCCCCTAA TGAAGAATTT GGCCCAGGTG TCCCAGCAGA AACTGATTTC 
ACAGAAACAA GCATCAATGG CCATGGCGCA TATGGAGAGA AAGGGCAGAA GGGGGAACCT 
GCTGTAGTTG AACCCGGAAT GCTTGTTGAA GGACCACCTG GGCCAGCTGG ACCAGCGGGT 
CTTATGGGTC CTCCAGGACT ACAAGGTCCT TCTGGGCTCC CCGGCGACCC TGGTGATAGG 
GGCCCACCAG GACGCCCTGG CTTACCAGGG GCTGATGGCT TACCCGGTCC TCCAGGAACC 
ATGTTAATGT TACCATTCCG CTATGGGGGT GACGGCTCCA AAGGACCAAC CATCTCTGCG 
CAGGAAGCCC AGGCTCAGGC GATTCTTCAG CAGGCTCGGA TTGCTCTGAG AGGCCCTCCT 
GGCCCAATGG GTCTTACCGG AAGACCAGGT CCTGTGGGGG GTCCTGGTTC AGCTGGAGCC 
AAAGGTGAGA GTGGGGATCC AGGTCCTCAG GGTCCTCGAG GTGTCCAAGG TCCTCCTGGT 
CCAACCGGAA AACCTGGAAA GAGGGGCCGT CCAGGTGCCG ATGGAGGAAG AGGCATGCCA 
GGAGAACCTG GGTCTAAGGG AGACCGAGGG TTTGATGGAC TTCCAGGTCT GCCAGGTGAC 
AAAGGTCACA GGGGGGAAAG AGGTCCACAA GGTCCACCTG GCCTCCCCGG CGATGATGGA 
ATGAGGGGAG AAGATGGAGA GATCGGGCCC AGGGGTCTTC CAGGGGAAGC TGGTCCACGA 
GGCTTGCTGG GACCAAGGGG CACTCCAGGA CCTCCTGGGC AGCCTGGTAT TGGAGGTATA 
GATGGCCCCC AAGGACCAAA AGGAAACATG GGTCCTCAAG GGGAGCCTGG ACCACCAGGT 
CAGCAAGGAA ATCCAGGACC TCAAGGACTT CCTGGTCCAC AAGGCCCAAT TGGTCCTCCT 
GGAGAAAAAG GACCACAAGG AAAACCAGGG CTTGCAGGAC TTCCTGGTGC CGATGGACCT 
CCTGGTCATC CTGGGAAAGA AGGCCAGTCT GGAGAAAAGG GTGCCCTGGG TCCTCCTGGT 
CCTCAGGGAC CTATTGGCTA TCCCGGTCCC CGTGGAGTAA AGGGAGCAGA TGGTGTCAGA 
GGTCTCAAGG GCTCTAAAGG AGAAAAGGGT GAAGATGGCT TTCCAGGATT CAAAGGTGAC 
ATGGGTCTTA AAGGTGACAG AGGCGAAGTG GGTCAAGTTG GTCCAAGAGG AGAAGATGGC 
CCTGAAGGCC CCAAAGGCCG TGCAGGCCCA ACTGGAGACC CAGGTCCCTC TGGCCAAGCA 
GGAGAGAAGG GAAAACTTGG GGTTCCAGGA TTGCCAGGAT ATCCAGGAAG ACAAGGTCCA 
AAGGGATCCA CTGGATTTCC TGGATTTCCA GGTGCCAACG GGGAGAAAGG TGCCCGGGGA 
ATTGCTGGCA AACCAGGCCC CCGAGGACAA CGTGGTCCAA CGGGTCCTCG AGGTTCCAGA 
GGCGCCAGAG GTCCCACAGG AAAACCTGGG CCGAAGGGTA CATCAGGTGG TGATGGCCCT 
CCTGGCCCAC CAGGCGAAAG GGGCCCTCAA GGACCTCAAG GTCCAGTTGG ATTCCCTGGA 
CCAAAAGGCC CCCCTGGCCC TGCTGGGAAA GATGGGCTAC CAGGACACCC TGGACAACGT 
GGAGAGACTG GATTTCAAGG AAAGACCGGT CCCCCTGGGC CAGGAGGTGT GGTAGGACCA 
CAGGGACCAA CTGGTGAGAC TGGCCCGATA GGTGAACGTG GGCATCCTGG CACTCCTGGT 
CCTCCGGGCG AGCAAGGACT TCCTGGTGCT GCAGGGAAAG AAGGTGCAAA GGGTGATCCT 
GGTCCTCAAG GTATCTCAGG GAAAGATGGA CCAGCAGGAA TCCGTGGTTT CCCAGGTGAA 
AGAGGCCTTC CTGGAGCCCA GGGCGCACCT GGGTTGAAAG GAGGAGAAGG TCCCCAAGGC 
CCGCAGGGTC CAATTGGCTC TCCTGGAGAG CGAGGCTCTG CAGGCACAGC AGGCCCAATT 
GGTTTGCCAG GACGTCCAGG CCCTCAGGGC CCTCCTGGTC CAGCTGGAGA AAAAGGTGCT 
CCGGGAGAAA AGGGTCCTCA GGGGCCTGCA GGTAGAGATG GGGTCCAAGG CCCTGTGGGT 
CTCCCAGGGC CTGCTGGTCC TGCTGGCTCT CCTGGAGAAG ATGGAGACAA GGGTGAAATT 
GGTGAACCAG GCCAAAAGGG CAGCAAAGGT GACAAAGGCG AAAATGGTCC TCCTGGTCCC 
CCGGGTCTTC AAGGCCCTGT TGGTGCTCCT GGAATTGCTG GAGGAGATGG TGAGGCAGGT 
CCCCGAGGAC AGCAGGGAAT GTTTGGACAA AAAGGTGATG AGGGTGCACG AGGTTTCCCA 
GGACCTCCTG GGCCCATAGG TCTTCAGGGG TTGCCAGGCC CACCAGGTGA AAAGGGCGAA 
AACGGAGACG TTGGCCCTAT GGGTCCACCA GGTCCTCCTG GCCCACGAGG CCCTCAAGGT 
CCCAATGGAG CTGATGGACC ACAAGGACCC CCAGGCTCTA TTGGTTCAGT TGGTGGTGTG 
GGAGAAAAGG GTGAACCAGG AGAAGCAGGG AACCCAGGAC CCCCTGGAGA AGCCGGATCA 
GGTGGTCCCA AAGGAGAGAG AGGAGAGAAA GGAGAAGCTG GGCCCCCTGG TGCTGCGGGT 
CCTCCTGGTA TTAAGGGGCC ACCAGGTGAT GATGGACCCA AGGGGAATCC GGGACCTGTT 
GGGTTTCCTG GAGATCCTGG TCCACCTGGA GAACCTGGTC CTGCAGGGCA AGATGGTGTT 
GGAGGTGACA AGGGTGAAGA TGGAGATCCA GGACAACCAG GTCCCCCTGG TCCATCCGGT 
GAAGCTGGCC CTCCAGGTCC ACCTGGAAAG AGAGGTCCGC CTGGAGCTTC CGGTTCCGAA 
GGAAGGCAAG GAGAGAAAGG TGCTAAGGGA GAAGCTGGAG CTGAAGGACC TCCTGGGAAA 
ACTGGCCCGG TCGGACCTCA GGGTCCTTCT GGAAAGCCTG GTCCAGAAGG TCTTCGAGGC 
ATCCCTGGTC CTGTGGGAGA ACAAGGTCTC CCCGGTGCTG CTGGCCAAGA TGGACCCCCT 
GGTCCTCTGG GACCTCCTGG TTTACCTGGT CTCAAAGGTG ATCCTGGCTC CAAGGGTGAA 
AAGGGACATC CTGGTTTAAT TGGATTGATT GGCCCTCCAG GAGAACAAGG GGAAAAGGGT 
GACAGAGGAC TTCCTGGGAC TCAAGGTTCT CCAGGAGCCA AAGGTGATGG GGGCATCCCA 
GGTCCTGCTG GTCCCATAGG TCCTCCTGGT CCTCCAGGAT TACCAGGTCC TGCTGGTCCG 
AAGGGTAACA AAGGATCTTC TGGACCTACT GGCCAGAAGG GTGACAGTGG TATGCCAGGA 
CCTCCTGGGC CTCCAGGTCC TCCTGGAGAA GTCATACAGC CATTACCTAT TTTGTCACCG 
AAGAAAACAA GAAGACACAC TGAAAGCATC CAGGCCGACG CAGGTGATAA TATTCTTGAC 
TACTCAGATG GCATGGAGGA AATATTTGGT TCCCTCAATT CTCTGAAACA AGACATTGAA 
CACATGAAGT TTCCCATGGG TACACAGACC AACCCAGCAC GAACATGCAA AGACCTGCAG 
CTCAGCCACC CCGACTTCCC AGATGGTGAA TATTGGATTG ATCCTAACCA AGGCTGCTCA 
GGTGATTCCT TCAAAGTTTA CTGTAATTTC ACAGCTGGTG GTGAGACGTG CATCTATCCA 
GACAAAAAAT CTGAGGGAGT AAGACTTTCA TCTTGGCCGA AGGAGAAACC AGGAAGTTGG 
TACAGTGAAT TTAAGAGAGG AAAACTGCTT TCCTATTTAG ATGTGGAAGG CAATTCCATC 
AATATGGTAC AAATGACATT CCTGAAACTC CTGACTGCCT CTGCCAGACA AAACTTCACC 
TACAATTGCC ACCAGTCAAC TGCTTGGTAT GACGTCTTAT CAGGAAGTTA TGACAAAGCA 
CTTCGGTTTC TGGGATCTAA TGATGAGGAA ATGTCCTACG AGAACAACCC ACACATCAAA 
GCTTTGTATG ACGGCTGTGC GTCCCGAAAA GGCTATGAAA AGACAGTGAT TGAGATCAAT 
ACTCCGAAAA TTGATCAAGT ACCCATCATT GATGTAATGA TCAATGATTT TGGTGATCAG 
AATCAGAAGT TTGGATTTGA AGTTGGTCCA GCTTGCTTTC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col11a1 ( XM_017590651.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446140.1 Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X3, mRNA.

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CloneID	ORa59636
Clone ID Related Accession (Same CDS sequence)	XM_017590651.1
Accession Version	XM_017590651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGTATGCA 
CCTGAGGATA TAATCGAGTA TGACTATGAA TATGGGGAGA CAGACTATAA AGAGGCTGAG 
AGTGTAACAG AGATGCCCAC TGTCACTGAA GAAACAGTAG CACAAACTGA GAAAAAGAAA 
TCCAATTACA CAAAGAAGAA GAGGACATTG GCCACTAACT CAAAGAAAAA ATCTAAAATG 
TCCACAACCC CCAAATCTGA AAAGTTTGCA TCCAAGAAGA AGAAACGTAA CCAAGCCACA 
GCCAAAGCCA AACTAGGGGT CCAGGCAAAT ATTGTGGATG ACTTTCAAGA CTATAACTAT 
GGAACGATGG AAACTTACCA GACTGAGTCT CCTCGGCGGG TATCAGGATC AAATGAGATC 
AATGGCCATG GCGCATATGG AGAGAAAGGG CAGAAGGGGG AACCTGCTGT AGTTGAACCC 
GGAATGCTTG TTGAAGGACC ACCTGGGCCA GCTGGACCAG CGGGTCTTAT GGGTCCTCCA 
GGACTACAAG GTCCTTCTGG GCTCCCCGGC GACCCTGGTG ATAGGGGCCC ACCAGGACGC 
CCTGGCTTAC CAGGGGCTGA TGGCTTACCC GGTCCTCCAG GAACCATGTT AATGTTACCA 
TTCCGCTATG GGGGTGACGG CTCCAAAGGA CCAACCATCT CTGCGCAGGA AGCCCAGGCT 
CAGGCGATTC TTCAGCAGGC TCGGATTGCT CTGAGAGGCC CTCCTGGCCC AATGGGTCTT 
ACCGGAAGAC CAGGTCCTGT GGGGGGTCCT GGTTCAGCTG GAGCCAAAGG TGAGAGTGGG 
GATCCAGGTC CTCAGGGTCC TCGAGGTGTC CAAGGTCCTC CTGGTCCAAC CGGAAAACCT 
GGAAAGAGGG GCCGTCCAGG TGCCGATGGA GGAAGAGGCA TGCCAGGAGA ACCTGGGTCT 
AAGGGAGACC GAGGGTTTGA TGGACTTCCA GGTCTGCCAG GTGACAAAGG TCACAGGGGG 
GAAAGAGGTC CACAAGGTCC ACCTGGCCTC CCCGGCGATG ATGGAATGAG GGGAGAAGAT 
GGAGAGATCG GGCCCAGGGG TCTTCCAGGG GAAGCTGGTC CACGAGGCTT GCTGGGACCA 
AGGGGCACTC CAGGACCTCC TGGGCAGCCT GGTATTGGAG GTATAGATGG CCCCCAAGGA 
CCAAAAGGAA ACATGGGTCC TCAAGGGGAG CCTGGACCAC CAGGTCAGCA AGGAAATCCA 
GGACCTCAAG GACTTCCTGG TCCACAAGGC CCAATTGGTC CTCCTGGAGA AAAAGGACCA 
CAAGGAAAAC CAGGGCTTGC AGGACTTCCT GGTGCCGATG GACCTCCTGG TCATCCTGGG 
AAAGAAGGCC AGTCTGGAGA AAAGGGTGCC CTGGGTCCTC CTGGTCCTCA GGGACCTATT 
GGCTATCCCG GTCCCCGTGG AGTAAAGGGA GCAGATGGTG TCAGAGGTCT CAAGGGCTCT 
AAAGGAGAAA AGGGTGAAGA TGGCTTTCCA GGATTCAAAG GTGACATGGG TCTTAAAGGT 
GACAGAGGCG AAGTGGGTCA AGTTGGTCCA AGAGGAGAAG ATGGCCCTGA AGGCCCCAAA 
GGCCGTGCAG GCCCAACTGG AGACCCAGGT CCCTCTGGCC AAGCAGGAGA GAAGGGAAAA 
CTTGGGGTTC CAGGATTGCC AGGATATCCA GGAAGACAAG GTCCAAAGGG ATCCACTGGA 
TTTCCTGGAT TTCCAGGTGC CAACGGGGAG AAAGGTGCCC GGGGAATTGC TGGCAAACCA 
GGCCCCCGAG GACAACGTGG TCCAACGGGT CCTCGAGGTT CCAGAGGCGC CAGAGGTCCC 
ACAGGAAAAC CTGGGCCGAA GGGTACATCA GGTGGTGATG GCCCTCCTGG CCCACCAGGC 
GAAAGGGGCC CTCAAGGACC TCAAGGTCCA GTTGGATTCC CTGGACCAAA AGGCCCCCCT 
GGCCCTGCTG GGAAAGATGG GCTACCAGGA CACCCTGGAC AACGTGGAGA GACTGGATTT 
CAAGGAAAGA CCGGTCCCCC TGGGCCAGGA GGTGTGGTAG GACCACAGGG ACCAACTGGT 
GAGACTGGCC CGATAGGTGA ACGTGGGCAT CCTGGCACTC CTGGTCCTCC GGGCGAGCAA 
GGACTTCCTG GTGCTGCAGG GAAAGAAGGT GCAAAGGGTG ATCCTGGTCC TCAAGGTATC 
TCAGGGAAAG ATGGACCAGC AGGAATCCGT GGTTTCCCAG GTGAAAGAGG CCTTCCTGGA 
GCCCAGGGCG CACCTGGGTT GAAAGGAGGA GAAGGTCCCC AAGGCCCGCA GGGTCCAATT 
GGCTCTCCTG GAGAGCGAGG CTCTGCAGGC ACAGCAGGCC CAATTGGTTT GCCAGGACGT 
CCAGGCCCTC AGGGCCCTCC TGGTCCAGCT GGAGAAAAAG GTGCTCCGGG AGAAAAGGGT 
CCTCAGGGGC CTGCAGGTAG AGATGGGGTC CAAGGCCCTG TGGGTCTCCC AGGGCCTGCT 
GGTCCTGCTG GCTCTCCTGG AGAAGATGGA GACAAGGGTG AAATTGGTGA ACCAGGCCAA 
AAGGGCAGCA AAGGTGACAA AGGCGAAAAT GGTCCTCCTG GTCCCCCGGG TCTTCAAGGC 
CCTGTTGGTG CTCCTGGAAT TGCTGGAGGA GATGGTGAGG CAGGTCCCCG AGGACAGCAG 
GGAATGTTTG GACAAAAAGG TGATGAGGGT GCACGAGGTT TCCCAGGACC TCCTGGGCCC 
ATAGGTCTTC AGGGGTTGCC AGGCCCACCA GGTGAAAAGG GCGAAAACGG AGACGTTGGC 
CCTATGGGTC CACCAGGTCC TCCTGGCCCA CGAGGCCCTC AAGGTCCCAA TGGAGCTGAT 
GGACCACAAG GACCCCCAGG CTCTATTGGT TCAGTTGGTG GTGTGGGAGA AAAGGGTGAA 
CCAGGAGAAG CAGGGAACCC AGGACCCCCT GGAGAAGCCG GATCAGGTGG TCCCAAAGGA 
GAGAGAGGAG AGAAAGGAGA AGCTGGGCCC CCTGGTGCTG CGGGTCCTCC TGGTATTAAG 
GGGCCACCAG GTGATGATGG ACCCAAGGGG AATCCGGGAC CTGTTGGGTT TCCTGGAGAT 
CCTGGTCCAC CTGGAGAACC TGGTCCTGCA GGGCAAGATG GTGTTGGAGG TGACAAGGGT 
GAAGATGGAG ATCCAGGACA ACCAGGTCCC CCTGGTCCAT CCGGTGAAGC TGGCCCTCCA 
GGTCCACCTG GAAAGAGAGG TCCGCCTGGA GCTTCCGGTT CCGAAGGAAG GCAAGGAGAG 
AAAGGTGCTA AGGGAGAAGC TGGAGCTGAA GGACCTCCTG GGAAAACTGG CCCGGTCGGA 
CCTCAGGGTC CTTCTGGAAA GCCTGGTCCA GAAGGTCTTC GAGGCATCCC TGGTCCTGTG 
GGAGAACAAG GTCTCCCCGG TGCTGCTGGC CAAGATGGAC CCCCTGGTCC TCTGGGACCT 
CCTGGTTTAC CTGGTCTCAA AGGTGATCCT GGCTCCAAGG GTGAAAAGGG ACATCCTGGT 
TTAATTGGAT TGATTGGCCC TCCAGGAGAA CAAGGGGAAA AGGGTGACAG AGGACTTCCT 
GGGACTCAAG GTTCTCCAGG AGCCAAAGGT GATGGGGGCA TCCCAGGTCC TGCTGGTCCC 
ATAGGTCCTC CTGGTCCTCC AGGATTACCA GGTCCTGCTG GTCCGAAGGG TAACAAAGGA 
TCTTCTGGAC CTACTGGCCA GAAGGGTGAC AGTGGTATGC CAGGACCTCC TGGGCCTCCA 
GGTCCTCCTG GAGAAGTCAT ACAGCCATTA CCTATTTTGT CACCGAAGAA AACAAGAAGA 
CACACTGAAA GCATCCAGGC CGACGCAGGT GATAATATTC TTGACTACTC AGATGGCATG 
GAGGAAATAT TTGGTTCCCT CAATTCTCTG AAACAAGACA TTGAACACAT GAAGTTTCCC 
ATGGGTACAC AGACCAACCC AGCACGAACA TGCAAAGACC TGCAGCTCAG CCACCCCGAC 
TTCCCAGATG GTGAATATTG GATTGATCCT AACCAAGGCT GCTCAGGTGA TTCCTTCAAA 
GTTTACTGTA ATTTCACAGC TGGTGGTGAG ACGTGCATCT ATCCAGACAA AAAATCTGAG 
GGAGTAAGAC TTTCATCTTG GCCGAAGGAG AAACCAGGAA GTTGGTACAG TGAATTTAAG 
AGAGGAAAAC TGCTTTCCTA TTTAGATGTG GAAGGCAATT CCATCAATAT GGTACAAATG 
ACATTCCTGA AACTCCTGAC TGCCTCTGCC AGACAAAACT TCACCTACAA TTGCCACCAG 
TCAACTGCTT GGTATGACGT CTTATCAGGA AGTTATGACA AAGCACTTCG GTTTCTGGGA 
TCTAATGATG AGGAAATGTC CTACGAGAAC AACCCACACA TCAAAGCTTT GTATGACGGC 
TGTGCGTCCC GAAAAGGCTA TGAAAAGACA GTGATTGAGA TCAATACTCC GAAAATTGAT 
CAAGTACCCA TCATTGATGT AATGATCAAT GATTTTGGTG ATCAGAATCA GAAGTTTGGA 
TTTGAAGTTG GTCCAGCTTG CTTTCTTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446140.1
CDS	358..5670
Translation	MEPWSRWKTKRWIWDLTISTLVLTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTSRKNSKDPDIAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFEDHTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRILETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEYAPEDIIEYDYEYGETDYKEAESVTEMPTVTEETVAQTEKKKSNYTKKKRTLATNSKKKSKMSTTPKSEKFASKKKKRNQATAKAKLGVQANIVDDFQDYNYGTMETYQTESPRRVSGSNEINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGTPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGPQGPIGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEAGPRGQQGMFGQKGDEGARGFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGGVGEKGEPGEAGNPGPPGEAGSGGPKGERGEKGEAGPPGAAGPPGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEVIQPLPILSPKKTRRHTESIQADAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRLSSWPKEKPGSWYSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSTAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKALYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_017590651.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XI alpha 1 chain (Col11a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590651.1

ORF Insert Sequence:

ATGGAGCCCT GGTCCAGGTG GAAAACGAAA CGGTGGATCT GGGATCTCAC CATCTCAACC 
CTCGTCTTGA CTTTTCTCTT CCAAGCCAGA GAGGTCAGAG GAGCTGCTCC AGTTGATATA 
CTGAAAGCAT TAGATTTTCA CAATTCTCCA GTGGGAATAT CTAAAACAAC AGGATTTTGC 
ACAAGCAGAA AGAATTCTAA AGATCCAGAT ATCGCTTATA GAGTTACCGA GGAAGCCCAG 
ATCAGTGCCC CAACGAAGCA GTTATTTCCA GGAGGAATTT TTCCGCAAGA TTTTTCAATA 
CTATTTACAA TAAAACCCAA AAAAGGAACT CAAGCTTTCC TCTTGTCTCT CTACAATGAA 
CATGGCATTC AGCAACTCGG TGTTGAGGTT GGGAGATCAC CTGTTTTTCT ATTTGAAGAC 
CACACTGGAA AACCCACACC AGAAAACTAT CCTCTCTTCT CAACAGTTAA CATTGCTGAT 
GGAAAGTGGC ACCGGGTAGC AATCAGTGTG GAGAAGAAAA CTGTGACAAT GATTGTCGAT 
TGTAAGAAGA AAATCACAAA ACCCCTCGAT AGAAGCGAGA GATCGATAGT CGATACCAAC 
GGAATCATGG TATTTGGAAC AAGAATTTTA GAAACAGATG TTTTCCAGGG TGATATTCAG 
CAGTTCCTGA TCACTGGTGA CCCCAAGGCA GCATATGACT ACTGTGACCA TTACAGTCCC 
GACTGTGACT TAACATCCAA GGCCGCTCAG GCCCAGGAGC CGCACATAGA TGAGTATGCA 
CCTGAGGATA TAATCGAGTA TGACTATGAA TATGGGGAGA CAGACTATAA AGAGGCTGAG 
AGTGTAACAG AGATGCCCAC TGTCACTGAA GAAACAGTAG CACAAACTGA GAAAAAGAAA 
TCCAATTACA CAAAGAAGAA GAGGACATTG GCCACTAACT CAAAGAAAAA ATCTAAAATG 
TCCACAACCC CCAAATCTGA AAAGTTTGCA TCCAAGAAGA AGAAACGTAA CCAAGCCACA 
GCCAAAGCCA AACTAGGGGT CCAGGCAAAT ATTGTGGATG ACTTTCAAGA CTATAACTAT 
GGAACGATGG AAACTTACCA GACTGAGTCT CCTCGGCGGG TATCAGGATC AAATGAGATC 
AATGGCCATG GCGCATATGG AGAGAAAGGG CAGAAGGGGG AACCTGCTGT AGTTGAACCC 
GGAATGCTTG TTGAAGGACC ACCTGGGCCA GCTGGACCAG CGGGTCTTAT GGGTCCTCCA 
GGACTACAAG GTCCTTCTGG GCTCCCCGGC GACCCTGGTG ATAGGGGCCC ACCAGGACGC 
CCTGGCTTAC CAGGGGCTGA TGGCTTACCC GGTCCTCCAG GAACCATGTT AATGTTACCA 
TTCCGCTATG GGGGTGACGG CTCCAAAGGA CCAACCATCT CTGCGCAGGA AGCCCAGGCT 
CAGGCGATTC TTCAGCAGGC TCGGATTGCT CTGAGAGGCC CTCCTGGCCC AATGGGTCTT 
ACCGGAAGAC CAGGTCCTGT GGGGGGTCCT GGTTCAGCTG GAGCCAAAGG TGAGAGTGGG 
GATCCAGGTC CTCAGGGTCC TCGAGGTGTC CAAGGTCCTC CTGGTCCAAC CGGAAAACCT 
GGAAAGAGGG GCCGTCCAGG TGCCGATGGA GGAAGAGGCA TGCCAGGAGA ACCTGGGTCT 
AAGGGAGACC GAGGGTTTGA TGGACTTCCA GGTCTGCCAG GTGACAAAGG TCACAGGGGG 
GAAAGAGGTC CACAAGGTCC ACCTGGCCTC CCCGGCGATG ATGGAATGAG GGGAGAAGAT 
GGAGAGATCG GGCCCAGGGG TCTTCCAGGG GAAGCTGGTC CACGAGGCTT GCTGGGACCA 
AGGGGCACTC CAGGACCTCC TGGGCAGCCT GGTATTGGAG GTATAGATGG CCCCCAAGGA 
CCAAAAGGAA ACATGGGTCC TCAAGGGGAG CCTGGACCAC CAGGTCAGCA AGGAAATCCA 
GGACCTCAAG GACTTCCTGG TCCACAAGGC CCAATTGGTC CTCCTGGAGA AAAAGGACCA 
CAAGGAAAAC CAGGGCTTGC AGGACTTCCT GGTGCCGATG GACCTCCTGG TCATCCTGGG 
AAAGAAGGCC AGTCTGGAGA AAAGGGTGCC CTGGGTCCTC CTGGTCCTCA GGGACCTATT 
GGCTATCCCG GTCCCCGTGG AGTAAAGGGA GCAGATGGTG TCAGAGGTCT CAAGGGCTCT 
AAAGGAGAAA AGGGTGAAGA TGGCTTTCCA GGATTCAAAG GTGACATGGG TCTTAAAGGT 
GACAGAGGCG AAGTGGGTCA AGTTGGTCCA AGAGGAGAAG ATGGCCCTGA AGGCCCCAAA 
GGCCGTGCAG GCCCAACTGG AGACCCAGGT CCCTCTGGCC AAGCAGGAGA GAAGGGAAAA 
CTTGGGGTTC CAGGATTGCC AGGATATCCA GGAAGACAAG GTCCAAAGGG ATCCACTGGA 
TTTCCTGGAT TTCCAGGTGC CAACGGGGAG AAAGGTGCCC GGGGAATTGC TGGCAAACCA 
GGCCCCCGAG GACAACGTGG TCCAACGGGT CCTCGAGGTT CCAGAGGCGC CAGAGGTCCC 
ACAGGAAAAC CTGGGCCGAA GGGTACATCA GGTGGTGATG GCCCTCCTGG CCCACCAGGC 
GAAAGGGGCC CTCAAGGACC TCAAGGTCCA GTTGGATTCC CTGGACCAAA AGGCCCCCCT 
GGCCCTGCTG GGAAAGATGG GCTACCAGGA CACCCTGGAC AACGTGGAGA GACTGGATTT 
CAAGGAAAGA CCGGTCCCCC TGGGCCAGGA GGTGTGGTAG GACCACAGGG ACCAACTGGT 
GAGACTGGCC CGATAGGTGA ACGTGGGCAT CCTGGCACTC CTGGTCCTCC GGGCGAGCAA 
GGACTTCCTG GTGCTGCAGG GAAAGAAGGT GCAAAGGGTG ATCCTGGTCC TCAAGGTATC 
TCAGGGAAAG ATGGACCAGC AGGAATCCGT GGTTTCCCAG GTGAAAGAGG CCTTCCTGGA 
GCCCAGGGCG CACCTGGGTT GAAAGGAGGA GAAGGTCCCC AAGGCCCGCA GGGTCCAATT 
GGCTCTCCTG GAGAGCGAGG CTCTGCAGGC ACAGCAGGCC CAATTGGTTT GCCAGGACGT 
CCAGGCCCTC AGGGCCCTCC TGGTCCAGCT GGAGAAAAAG GTGCTCCGGG AGAAAAGGGT 
CCTCAGGGGC CTGCAGGTAG AGATGGGGTC CAAGGCCCTG TGGGTCTCCC AGGGCCTGCT 
GGTCCTGCTG GCTCTCCTGG AGAAGATGGA GACAAGGGTG AAATTGGTGA ACCAGGCCAA 
AAGGGCAGCA AAGGTGACAA AGGCGAAAAT GGTCCTCCTG GTCCCCCGGG TCTTCAAGGC 
CCTGTTGGTG CTCCTGGAAT TGCTGGAGGA GATGGTGAGG CAGGTCCCCG AGGACAGCAG 
GGAATGTTTG GACAAAAAGG TGATGAGGGT GCACGAGGTT TCCCAGGACC TCCTGGGCCC 
ATAGGTCTTC AGGGGTTGCC AGGCCCACCA GGTGAAAAGG GCGAAAACGG AGACGTTGGC 
CCTATGGGTC CACCAGGTCC TCCTGGCCCA CGAGGCCCTC AAGGTCCCAA TGGAGCTGAT 
GGACCACAAG GACCCCCAGG CTCTATTGGT TCAGTTGGTG GTGTGGGAGA AAAGGGTGAA 
CCAGGAGAAG CAGGGAACCC AGGACCCCCT GGAGAAGCCG GATCAGGTGG TCCCAAAGGA 
GAGAGAGGAG AGAAAGGAGA AGCTGGGCCC CCTGGTGCTG CGGGTCCTCC TGGTATTAAG 
GGGCCACCAG GTGATGATGG ACCCAAGGGG AATCCGGGAC CTGTTGGGTT TCCTGGAGAT 
CCTGGTCCAC CTGGAGAACC TGGTCCTGCA GGGCAAGATG GTGTTGGAGG TGACAAGGGT 
GAAGATGGAG ATCCAGGACA ACCAGGTCCC CCTGGTCCAT CCGGTGAAGC TGGCCCTCCA 
GGTCCACCTG GAAAGAGAGG TCCGCCTGGA GCTTCCGGTT CCGAAGGAAG GCAAGGAGAG 
AAAGGTGCTA AGGGAGAAGC TGGAGCTGAA GGACCTCCTG GGAAAACTGG CCCGGTCGGA 
CCTCAGGGTC CTTCTGGAAA GCCTGGTCCA GAAGGTCTTC GAGGCATCCC TGGTCCTGTG 
GGAGAACAAG GTCTCCCCGG TGCTGCTGGC CAAGATGGAC CCCCTGGTCC TCTGGGACCT 
CCTGGTTTAC CTGGTCTCAA AGGTGATCCT GGCTCCAAGG GTGAAAAGGG ACATCCTGGT 
TTAATTGGAT TGATTGGCCC TCCAGGAGAA CAAGGGGAAA AGGGTGACAG AGGACTTCCT 
GGGACTCAAG GTTCTCCAGG AGCCAAAGGT GATGGGGGCA TCCCAGGTCC TGCTGGTCCC 
ATAGGTCCTC CTGGTCCTCC AGGATTACCA GGTCCTGCTG GTCCGAAGGG TAACAAAGGA 
TCTTCTGGAC CTACTGGCCA GAAGGGTGAC AGTGGTATGC CAGGACCTCC TGGGCCTCCA 
GGTCCTCCTG GAGAAGTCAT ACAGCCATTA CCTATTTTGT CACCGAAGAA AACAAGAAGA 
CACACTGAAA GCATCCAGGC CGACGCAGGT GATAATATTC TTGACTACTC AGATGGCATG 
GAGGAAATAT TTGGTTCCCT CAATTCTCTG AAACAAGACA TTGAACACAT GAAGTTTCCC 
ATGGGTACAC AGACCAACCC AGCACGAACA TGCAAAGACC TGCAGCTCAG CCACCCCGAC 
TTCCCAGATG GTGAATATTG GATTGATCCT AACCAAGGCT GCTCAGGTGA TTCCTTCAAA 
GTTTACTGTA ATTTCACAGC TGGTGGTGAG ACGTGCATCT ATCCAGACAA AAAATCTGAG 
GGAGTAAGAC TTTCATCTTG GCCGAAGGAG AAACCAGGAA GTTGGTACAG TGAATTTAAG 
AGAGGAAAAC TGCTTTCCTA TTTAGATGTG GAAGGCAATT CCATCAATAT GGTACAAATG 
ACATTCCTGA AACTCCTGAC TGCCTCTGCC AGACAAAACT TCACCTACAA TTGCCACCAG 
TCAACTGCTT GGTATGACGT CTTATCAGGA AGTTATGACA AAGCACTTCG GTTTCTGGGA 
TCTAATGATG AGGAAATGTC CTACGAGAAC AACCCACACA TCAAAGCTTT GTATGACGGC 
TGTGCGTCCC GAAAAGGCTA TGAAAAGACA GTGATTGAGA TCAATACTCC GAAAATTGAT 
CAAGTACCCA TCATTGATGT AATGATCAAT GATTTTGGTG ATCAGAATCA GAAGTTTGGA 
TTTGAAGTTG GTCCAGCTTG CTTTCTTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Changes in type II procollagen isoform expression during chondrogenesis by disruption of an alternative 5' splice site within Col2a1 exon 2.
Matrix biology : journal of the International Society for Matrix Biology3651-63(2014 Jun)
Hering TM,Wirthlin L,Ravindran S,McAlinden A

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Cell death and differentiation20(3)369-81(2013 Mar)
Brafman DA,Phung C,Kumar N,Willert K

Regulation of collagen fibril nucleation and initial fibril assembly involves coordinate interactions with collagens V and XI in developing tendon.
The Journal of biological chemistry286(23)20455-65(2011 Jun)
Wenstrup RJ,Smith SM,Florer JB,Zhang G,Beason DP,Seegmiller RE,Soslowsky LJ,Birk DE

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Col11a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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