Mc4r cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mc4r
Entrez Gene ID	25635
Full Name	melanocortin 4 receptor
General protein information	Preferred Names melanocortin 4 receptor Names melanocortin receptor 4 MC4-R
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.1
Summary	receptor involved in maintaining energy homeostasis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013099.3	NP_037231.1	melanocortin receptor 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MC4R	NP_776535.1
Mus musculus (house mouse)	Mc4r	NP_058673.2
Xenopus tropicalis (tropical clawed frog)	mc4r	XP_004915370.1
Homo sapiens (human)	MC4R	NP_005903.2
Canis lupus familiaris (dog)	MC4R	NP_001074193.1
	MC4R	XP_001088600.1
Pan troglodytes (chimpanzee)	MC4R	NP_001153207.1
Rattus norvegicus (Norway rat)	Mc4r	NP_037231.1
Gallus gallus (chicken)	MC4R	NP_001026685.1
Danio rerio (zebrafish)	mc4r	NP_775385.1

Gene Ontology
Bibliography

Related articles in PubMed

Melanocortin 4 Receptor Activation Attenuates Mitochondrial Dysfunction in Skeletal Muscle of Diabetic Rats.
Zhang HH, Liu J, Qin GJ, Li XL, Du PJ, Hao X, Zhao D, Tian T, Wu J, Yun M, Bai YH
Journal of cellular biochemistry118(11)4072-4079(2017 Nov)

Adolescent Alcohol Exposure-Induced Changes in Alpha-Melanocyte Stimulating Hormone and Neuropeptide Y Pathways via Histone Acetylation in the Brain During Adulthood.
Kokare DM, Kyzar EJ, Zhang H, Sakharkar AJ, Pandey SC
The international journal of neuropsychopharmacology20(9)758-768(2017 Sep)

Nitric oxide synthase-mediated blood pressure regulation in obese melanocortin-4 receptor-deficient pregnant rats.
Spradley FT, Sasser JM, Musall JB, Sullivan JC, Granger JP
American journal of physiology. Regulatory, integrative and comparative physiology311(5)R851-R857(2016 Nov)

Effects of Melanocortin 3 and 4 Receptor Deficiency on Energy Homeostasis in Rats.
You P, Hu H, Chen Y, Zhao Y, Yang Y, Wang T, Xing R, Shao Y, Zhang W, Li D, Chen H, Liu M
Scientific reports634938(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results revealed that MC4R activation was able to attenuate oxidative stress and mitochondrial dysfunction in skeletal muscle induced by diabetes partially through activating the AMPK-SIRT1-PGC-1alpha signaling pathway.
Title: Melanocortin 4 Receptor Activation Attenuates Mitochondrial Dysfunction in Skeletal Muscle of Diabetic Rats.

Although MC4R obese pregnant rats had elevated blood pressure associated with reduced total NOS activity and expression, they had enhanced NOS-mediated attenuation of vasoconstriction, with no evidence of alterations in NOS-mediated regulation of blood pressure.
Title: Nitric oxide synthase-mediated blood pressure regulation in obese melanocortin-4 receptor-deficient pregnant rats.

Data show that selective activation of melanocortin 4 receptor (MC4R) decreased levels of TLR4, HMGB1 and caspase-3 mRNA in liver tissues, and significantly downregulated positive rate of NF-kappaB p65 in hepatocytes.
Title: [Selectively activating melanocortin 4 receptor acts against rat sepsis-induced acute liver injury via HMGB1/TLR4/NF-?B signaling pathway].

A synthetic peptide corresponding to cytoplasmic loop 1 of MC4R suppresses basal adenylate cyclase activity and basal level of GppNHp binding of heterotrimeric G proteins produced by THIQ and alpha-MSH in synaptosomal membranes of rat brain.
Title: Regulation of the Melanocortin-Sensitive Adenylate Cyclase System by N-Acylated Peptide 71-82 of Type 4 Melanocortin Receptor.

in rats immunized with M4R and M3R peptides the sensitivity of ACSS thyrocytes to TSH were decreased, whereas in rats iimunized with 5-HT1BR peptide the deficit of thyroid hormones was associated with decreased level of TSH
Title: [THE THYROID STATUS OF RATS IMMUNIZED WITH PEPTIDES DERIVED FROM THE EXTRACELLULAR REGIONS OF THE TYPES 3 AND 4 MELANOCORTIN RECEPTORS AND THE 1B-SUBTYPE 5-HYDROXYTRYPTAMINE RECEPTOR].

The following Mc4r gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mc4r cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa53307	NM_013099.3 Latest version!	Rattus norvegicus melanocortin 4 receptor (Mc4r), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mc4r ( NM_013099.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037231.1 Rattus norvegicus melanocortin 4 receptor (Mc4r), mRNA.

Price & Availability↑

CloneID	ORa53307
Clone ID Related Accession (Same CDS sequence)	NM_013099.3
Accession Version	NM_013099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Rattus norvegicus(Norway rat)
Product	melanocortin receptor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07032359.1. On Dec 23, 2015 this sequence version replaced NM_013099.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGAACTCCA CCCACCACCA TGGCATGTAT ACTTCCCTCC ACCTCTGGAA CCGCAGCAGC 
CACGGGCTGC ACGGCAATGC CAGCGAGTCT CTGGGGAAGG GGCACTCAGA CGGAGGATGC 
TATGAGCAAC TTTTTGTCTC CCCCGAGGTG TTTGTGACTC TGGGTGTCAT AAGCCTGTTG 
GAGAACATTC TAGTGATCGT GGCGATAGCC AAGAACAAGA ACCTGCACTC ACCCATGTAC 
TTTTTCATCT GTAGTCTGGC TGTGGCGGAC ATGCTGGTGA GCGTTTCGAA CGGGTCAGAA 
ACCATCGTCA TCACCCTGCT AAACAGTACG GACACGGACG CCCAGAGCTT CACCGTGAAT 
ATTGATAATG TCATTGACTC TGTGATCTGT AGCTCCTTGC TCGCATCCAT TTGCAGCCTG 
CTTTCCATTG CAGTGGACAG GTATTTCACT ATCTTTTACG CGCTCCAGTA CCATAACATT 
ATGACGGTTA GGCGGGTCGG GATCATCATC AGTTGTATCT GGGCAGCTTG CACAGTATCG 
GGCGTTCTTT TTATCATTTA CTCGGACAGC AGCGCTGTCA TCATCTGCCT CATTACCATG 
TTCTTCACCA TGCTGGTTCT CATGGCCTCT CTCTATGTCC ACATGTTCCT GATGGCGAGG 
CTTCACATTA AGAGGATCGC TGTCCTCCCG GGCACGGGTA CCATCCGTCA GGGTGCCAAC 
ATGAAGGGCG CAATTACCTT GACCATTCTG ATTGGAGTGT TTGTTGTCTG CTGGGCCCCG 
TTTTTCCTCC ATTTACTGTT CTACATCTCT TGTCCTCAGA ATCCATACTG CGTGTGCTTC 
ATGTCTCATT TTAACTTGTA TCTCATACTG ATCATGTGTA ACGCTGTCAT CGACCCTCTC 
ATTTATGCCC TGCGGAGTCA AGAACTGAGG AAAACCTTCA AAGAGATCAT CTGTTTCTAC 
CCCCTGGGAG GCATCTGTGA GTTACCTGGC AGGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037231.1
CDS	409..1407
Translation	MNSTHHHGMYTSLHLWNRSSHGLHGNASESLGKGHSDGGCYEQLFVSPEVFVTLGVISLLENILVIVAIAKNKNLHSPMYFFICSLAVADMLVSVSNGSETIVITLLNSTDTDAQSFTVNIDNVIDSVICSSLLASICSLLSIAVDRYFTIFYALQYHNIMTVRRVGIIISCIWAACTVSGVLFIIYSDSSAVIICLITMFFTMLVLMASLYVHMFLMARLHIKRIAVLPGTGTIRQGANMKGAITLTILIGVFVVCWAPFFLHLLFYISCPQNPYCVCFMSHFNLYLILIMCNAVIDPLIYALRSQELRKTFKEIICFYPLGGICELPGRY

Target ORF information:

RefSeq Version	NM_013099.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus melanocortin 4 receptor (Mc4r), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013099.3

ORF Insert Sequence:

ATGAACTCCA CCCACCACCA TGGCATGTAT ACTTCCCTCC ACCTCTGGAA CCGCAGCAGC 
CACGGGCTGC ACGGCAATGC CAGCGAGTCT CTGGGGAAGG GGCACTCAGA CGGAGGATGC 
TATGAGCAAC TTTTTGTCTC CCCCGAGGTG TTTGTGACTC TGGGTGTCAT AAGCCTGTTG 
GAGAACATTC TAGTGATCGT GGCGATAGCC AAGAACAAGA ACCTGCACTC ACCCATGTAC 
TTTTTCATCT GTAGTCTGGC TGTGGCGGAC ATGCTGGTGA GCGTTTCGAA CGGGTCAGAA 
ACCATCGTCA TCACCCTGCT AAACAGTACG GACACGGACG CCCAGAGCTT CACCGTGAAT 
ATTGATAATG TCATTGACTC TGTGATCTGT AGCTCCTTGC TCGCATCCAT TTGCAGCCTG 
CTTTCCATTG CAGTGGACAG GTATTTCACT ATCTTTTACG CGCTCCAGTA CCATAACATT 
ATGACGGTTA GGCGGGTCGG GATCATCATC AGTTGTATCT GGGCAGCTTG CACAGTATCG 
GGCGTTCTTT TTATCATTTA CTCGGACAGC AGCGCTGTCA TCATCTGCCT CATTACCATG 
TTCTTCACCA TGCTGGTTCT CATGGCCTCT CTCTATGTCC ACATGTTCCT GATGGCGAGG 
CTTCACATTA AGAGGATCGC TGTCCTCCCG GGCACGGGTA CCATCCGTCA GGGTGCCAAC 
ATGAAGGGCG CAATTACCTT GACCATTCTG ATTGGAGTGT TTGTTGTCTG CTGGGCCCCG 
TTTTTCCTCC ATTTACTGTT CTACATCTCT TGTCCTCAGA ATCCATACTG CGTGTGCTTC 
ATGTCTCATT TTAACTTGTA TCTCATACTG ATCATGTGTA ACGCTGTCAT CGACCCTCTC 
ATTTATGCCC TGCGGAGTCA AGAACTGAGG AAAACCTTCA AAGAGATCAT CTGTTTCTAC 
CCCCTGGGAG GCATCTGTGA GTTACCTGGC AGGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Melanocortin 4 Receptor Activation Attenuates Mitochondrial Dysfunction in Skeletal Muscle of Diabetic Rats.
Journal of cellular biochemistry118(11)4072-4079(2017 Nov)
Zhang HH,Liu J,Qin GJ,Li XL,Du PJ,Hao X,Zhao D,Tian T,Wu J,Yun M,Bai YH

Adolescent Alcohol Exposure-Induced Changes in Alpha-Melanocyte Stimulating Hormone and Neuropeptide Y Pathways via Histone Acetylation in the Brain During Adulthood.
The international journal of neuropsychopharmacology20(9)758-768(2017 Sep)
Kokare DM,Kyzar EJ,Zhang H,Sakharkar AJ,Pandey SC

Nitric oxide synthase-mediated blood pressure regulation in obese melanocortin-4 receptor-deficient pregnant rats.
American journal of physiology. Regulatory, integrative and comparative physiology311(5)R851-R857(2016 Nov)
Spradley FT,Sasser JM,Musall JB,Sullivan JC,Granger JP

Effects of Melanocortin 3 and 4 Receptor Deficiency on Energy Homeostasis in Rats.
Scientific reports634938(2016 Oct)
You P,Hu H,Chen Y,Zhao Y,Yang Y,Wang T,Xing R,Shao Y,Zhang W,Li D,Chen H,Liu M

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Mc4r cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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