Prkag1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkag1
Entrez Gene ID	25520
Full Name	protein kinase AMP-activated non-catalytic subunit gamma 1
Synonyms	Prkaac,Prkga1
General protein information	Preferred Names protein kinase AMP-activated non-catalytic subunit gamma 1 Names 5'-AMP-activated protein kinase subunit gamma-1 AMP-activated protein kinase, noncatalytic gamma-1 subunit AMPK gamma-1 chain AMPK gamma1 AMPK subunit gamma-1 AMPKg Protein kinase AMP-activated gamma protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	non-catalytic component of the heterotrimeric AMP-activated protein kinase; may phosphorylate and inactivate enzymes involved in lipid metabolism; may play a role in response to metabolic stress [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257331.3	XP_006257393.1	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
NM_013010.2	NP_037142.1	5'-AMP-activated protein kinase subunit gamma-1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKAG1	NP_001029999.1
Caenorhabditis elegans (roundworm)	aakg-1	NP_499637.2
Oryza sativa (rice)	Os04g0401300	NP_001052687.2
Xenopus tropicalis (tropical clawed frog)	prkag2	NP_001096506.1
Mus musculus (house mouse)	Prkag1	NP_058061.2
Homo sapiens (human)	PRKAG1	NP_001193638.1
Canis lupus familiaris (dog)	PRKAG1	XP_543685.2
Pan troglodytes (chimpanzee)	PRKAG1	XP_003313774.1
	PRKAG1	XP_001105473.1
Rattus norvegicus (Norway rat)	Prkag1	NP_037142.1
Danio rerio (zebrafish)	prkag1	NP_998326.1
Bos taurus (cattle)	PRKAG1	NP_777011.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators.
Dopie J, Rajakyl? EK, Joensuu MS, Huet G, Ferrantelli E, Xie T, J??linoja H, Jokitalo E, Vartiainen MK
Journal of cell science128(13)2388-400(2015 Jul)

Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.
Zhu L, Chen L, Zhou XM, Zhang YY, Zhang YJ, Zhao J, Ji SR, Wu JW, Wu Y
Structure (London, England : 1993)19(4)515-22(2011 Apr)

Structure of mammalian AMPK and its regulation by ADP.
Xiao B, Sanders MJ, Underwood E, Heath R, Mayer FV, Carmena D, Jing C, Walker PA, Eccleston JF, Haire LF, Saiu P, Howell SA, Aasland R, Martin SR, Carling D, Gamblin SJ
Nature472(7342)230-3(2011 Apr)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Dietary energy restriction modulates the activity of AMP-activated protein kinase, Akt, and mammalian target of rapamycin in mammary carcinomas, mammary gland, and liver.
Jiang W, Zhu Z, Thompson HJ
Cancer research68(13)5492-9(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies determine the three-dimensional reconstruction and subunit organization of the full-length rat AMPK (alpha1beta1gamma1) through single-particle electron
Title: Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.

Dietary energy restriction modulates the activity of AMP-activated protein kinase in mammary carcinomas, mammary gland, and liver.
Title: Dietary energy restriction modulates the activity of AMP-activated protein kinase, Akt, and mammalian target of rapamycin in mammary carcinomas, mammary gland, and liver.

In pancreatic beta cells, expression of a constitutively active form of Akt increases mTOR activity and prevents apoptosis upon AMP-activated protein kinase activation.
Title: Akt activation protects pancreatic beta cells from AMPK-mediated death through stimulation of mTOR.

results indicate that ischemia activates catalytic alpha1 and alpha2 isoforms similarly in gamma1 and gamma2 isoform-containing AMPK complexes and that gamma1-isoform complexes account for most of the AMPK activity in the ischemic heart
Title: Activation of AMPK alpha- and gamma-isoform complexes in the intact ischemic rat heart.

Leptin and insulin deficiencies in diabetes lead to increased hypothalamic AMP activated protein kinase which contributes to the development of diabetic hyperphagia.
Title: Enhanced hypothalamic AMP-activated protein kinase activity contributes to hyperphagia in diabetic rats.

The following Prkag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01641	NM_013010.2 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit gamma 1 (Prkag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa40118	XM_006257331.3 Latest version!	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit gamma 1 (Prkag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkag1 ( NM_013010.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037142.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit gamma 1 (Prkag1), mRNA.

Price & Availability↑

CloneID	ORa01641
Clone ID Related Accession (Same CDS sequence)	NM_013010.2
Accession Version	NM_013010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit gamma-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097940.1. On Jun 19, 2005 this sequence version replaced NM_013010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC097940.1, X95578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTCGG TTGCTGCAGA GAGCGCTCCA GCTCCGGAGA ATGAACACTC TCAAGAGACC 
CCGGAATCGA ACAGTAGTGT GTACACCACC TTCATGAAGT CTCATCGCTG CTATGACCTG 
ATCCCCACAA GCTCCAAGCT GGTGGTATTT GATACTTCGC TGCAGGTAAA GAAAGCCTTC 
TTTGCCCTGG TGACTAACGG TGTTCGTGCT GCCCCTTTGT GGGATAGTAA GAAGCAGAGC 
TTTGTGGGCA TGCTGACCAT CACTGACTTC ATCAATATTC TGCACCGATA CTACAAGTCA 
GCCCTGGTGC AGATCTATGA ACTGGAGGAG CACAAGATAG AGACTTGGAG AGAGGTCTAC 
CTGCAAGACT CCTTTAAGCC ACTTGTCTGC ATTTCTCCAA ATGCCAGCTT GTTCGATGCT 
GTCTCTTCAT TAATTCGAAA TAAGATCCAC AGGCTTCCAG TTATTGACCC GGAGTCAGGC 
AACACCTTGT ACATTCTTAC TCACAAGCGG ATCCTCAAGT TCCTCAAGTT GTTTATCACT 
GAGTTCCCCA AGCCGGAATT CATGTCTAAG TCTCTGGAAG AGCTACAGAT TGGCACCTAC 
GCCAATATTG CCATGGTCCG TACCACTACA CCTGTCTATG TGGCTCTGGG CATCTTTGTA 
CAGCACCGAG TCTCCGCCTT GCCTGTGGTG GATGAGAAAG GGCGTGTGGT GGACATCTAC 
TCCAAGTTTG ATGTGATTAA TTTGGCAGCA GAAAAGACAT ACAACAACCT AGATGTGTCT 
GTGACAAAAG CCCTACAGCA CCGGTCACAC TACTTCGAGG GTGTTCTCAA GTGCTACCTA 
CATGAGACTC TAGAAGCAAT CATCAATAGA CTGGTGGAAG CAGAGGTTCA CCGTCTGGTG 
GTGGTGGATG AACATGACGT GGTCAAGGGC ATTGTATCGC TGTCTGACAT CTTACAGGCT 
CTGGTGCTCA CAGGTGGAGA GAAGAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037142.1
CDS	17..1009
Translation	MESVAAESAPAPENEHSQETPESNSSVYTTFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLEAIINRLVEAEVHRLVVVDEHDVVKGIVSLSDILQALVLTGGEKKP

Target ORF information:

RefSeq Version	NM_013010.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit gamma 1 (Prkag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013010.2

ORF Insert Sequence:

ATGGAGTCGG TTGCTGCAGA GAGCGCTCCA GCTCCGGAGA ATGAACACTC TCAAGAGACC 
CCGGAATCGA ACAGTAGTGT GTACACCACC TTCATGAAGT CTCATCGCTG CTATGACCTG 
ATCCCCACAA GCTCCAAGCT GGTGGTATTT GATACTTCGC TGCAGGTAAA GAAAGCCTTC 
TTTGCCCTGG TGACTAACGG TGTTCGTGCT GCCCCTTTGT GGGATAGTAA GAAGCAGAGC 
TTTGTGGGCA TGCTGACCAT CACTGACTTC ATCAATATTC TGCACCGATA CTACAAGTCA 
GCCCTGGTGC AGATCTATGA ACTGGAGGAG CACAAGATAG AGACTTGGAG AGAGGTCTAC 
CTGCAAGACT CCTTTAAGCC ACTTGTCTGC ATTTCTCCAA ATGCCAGCTT GTTCGATGCT 
GTCTCTTCAT TAATTCGAAA TAAGATCCAC AGGCTTCCAG TTATTGACCC GGAGTCAGGC 
AACACCTTGT ACATTCTTAC TCACAAGCGG ATCCTCAAGT TCCTCAAGTT GTTTATCACT 
GAGTTCCCCA AGCCGGAATT CATGTCTAAG TCTCTGGAAG AGCTACAGAT TGGCACCTAC 
GCCAATATTG CCATGGTCCG TACCACTACA CCTGTCTATG TGGCTCTGGG CATCTTTGTA 
CAGCACCGAG TCTCCGCCTT GCCTGTGGTG GATGAGAAAG GGCGTGTGGT GGACATCTAC 
TCCAAGTTTG ATGTGATTAA TTTGGCAGCA GAAAAGACAT ACAACAACCT AGATGTGTCT 
GTGACAAAAG CCCTACAGCA CCGGTCACAC TACTTCGAGG GTGTTCTCAA GTGCTACCTA 
CATGAGACTC TAGAAGCAAT CATCAATAGA CTGGTGGAAG CAGAGGTTCA CCGTCTGGTG 
GTGGTGGATG AACATGACGT GGTCAAGGGC ATTGTATCGC TGTCTGACAT CTTACAGGCT 
CTGGTGCTCA CAGGTGGAGA GAAGAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkag1 ( XM_006257331.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257393.1 Rattus norvegicus protein kinase AMP-activated non-catalytic subunit gamma 1 (Prkag1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa40118
Clone ID Related Accession (Same CDS sequence)	XM_006257331.3
Accession Version	XM_006257331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTC ATCGCTGCTA TGACCTGATC CCCACAAGCT CCAAGCTGGT GGTATTTGAT 
ACTTCGCTGC AGGTAAAGAA AGCCTTCTTT GCCCTGGTGA CTAACGGTGT TCGTGCTGCC 
CCTTTGTGGG ATAGTAAGAA GCAGAGCTTT GTGGGCATGC TGACCATCAC TGACTTCATC 
AATATTCTGC ACCGATACTA CAAGTCAGCC CTGGTGCAGA TCTATGAACT GGAGGAGCAC 
AAGATAGAGA CTTGGAGAGA GGTCTACCTG CAAGACTCCT TTAAGCCACT TGTCTGCATT 
TCTCCAAATG CCAGCTTGTT CGATGCTGTC TCTTCATTAA TTCGAAATAA GATCCACAGG 
CTTCCAGTTA TTGACCCGGA GTCAGGCAAC ACCTTGTACA TTCTTACTCA CAAGCGGATC 
CTCAAGTTCC TCAAGTTGTT TATCACTGAG TTCCCCAAGC CGGAATTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTACGCC AATATTGCCA TGGTCCGTAC CACTACACCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CCGCCTTGCC TGTGGTGGAT 
GAGAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TGATTAATTT GGCAGCAGAA 
AAGACATACA ACAACCTAGA TGTGTCTGTG ACAAAAGCCC TACAGCACCG GTCACACTAC 
TTCGAGGGTG TTCTCAAGTG CTACCTACAT GAGACTCTAG AAGCAATCAT CAATAGACTG 
GTGGAAGCAG AGGTTCACCG TCTGGTGGTG GTGGATGAAC ATGACGTGGT CAAGGGCATT 
GTATCGCTGT CTGACATCTT ACAGGCTCTG GTGCTCACAG GTGGAGAGAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257393.1
CDS	311..1210
Translation	MKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLEAIINRLVEAEVHRLVVVDEHDVVKGIVSLSDILQALVLTGGEKKP

Target ORF information:

RefSeq Version	XM_006257331.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase AMP-activated non-catalytic subunit gamma 1 (Prkag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257331.3

ORF Insert Sequence:

ATGAAGTCTC ATCGCTGCTA TGACCTGATC CCCACAAGCT CCAAGCTGGT GGTATTTGAT 
ACTTCGCTGC AGGTAAAGAA AGCCTTCTTT GCCCTGGTGA CTAACGGTGT TCGTGCTGCC 
CCTTTGTGGG ATAGTAAGAA GCAGAGCTTT GTGGGCATGC TGACCATCAC TGACTTCATC 
AATATTCTGC ACCGATACTA CAAGTCAGCC CTGGTGCAGA TCTATGAACT GGAGGAGCAC 
AAGATAGAGA CTTGGAGAGA GGTCTACCTG CAAGACTCCT TTAAGCCACT TGTCTGCATT 
TCTCCAAATG CCAGCTTGTT CGATGCTGTC TCTTCATTAA TTCGAAATAA GATCCACAGG 
CTTCCAGTTA TTGACCCGGA GTCAGGCAAC ACCTTGTACA TTCTTACTCA CAAGCGGATC 
CTCAAGTTCC TCAAGTTGTT TATCACTGAG TTCCCCAAGC CGGAATTCAT GTCTAAGTCT 
CTGGAAGAGC TACAGATTGG CACCTACGCC AATATTGCCA TGGTCCGTAC CACTACACCT 
GTCTATGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CCGCCTTGCC TGTGGTGGAT 
GAGAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TGATTAATTT GGCAGCAGAA 
AAGACATACA ACAACCTAGA TGTGTCTGTG ACAAAAGCCC TACAGCACCG GTCACACTAC 
TTCGAGGGTG TTCTCAAGTG CTACCTACAT GAGACTCTAG AAGCAATCAT CAATAGACTG 
GTGGAAGCAG AGGTTCACCG TCTGGTGGTG GTGGATGAAC ATGACGTGGT CAAGGGCATT 
GTATCGCTGT CTGACATCTT ACAGGCTCTG GTGCTCACAG GTGGAGAGAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators.
Journal of cell science128(13)2388-400(2015 Jul)
Dopie J,Rajakyl? EK,Joensuu MS,Huet G,Ferrantelli E,Xie T,J??linoja H,Jokitalo E,Vartiainen MK

Structural insights into the architecture and allostery of full-length AMP-activated protein kinase.
Structure (London, England : 1993)19(4)515-22(2011 Apr)
Zhu L,Chen L,Zhou XM,Zhang YY,Zhang YJ,Zhao J,Ji SR,Wu JW,Wu Y

Structure of mammalian AMPK and its regulation by ADP.
Nature472(7342)230-3(2011 Apr)
Xiao B,Sanders MJ,Underwood E,Heath R,Mayer FV,Carmena D,Jing C,Walker PA,Eccleston JF,Haire LF,Saiu P,Howell SA,Aasland R,Martin SR,Carling D,Gamblin SJ

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Dietary energy restriction modulates the activity of AMP-activated protein kinase, Akt, and mammalian target of rapamycin in mammary carcinomas, mammary gland, and liver.
Cancer research68(13)5492-9(2008 Jul)
Jiang W,Zhu Z,Thompson HJ

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Prkag1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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