Prkag1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkag1
Entrez Gene ID	19082
Full Name	protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Synonyms	AA571379,BB036179,Prkaac
General protein information	Preferred Names protein kinase, AMP-activated, gamma 1 non-catalytic subunit Names 5'-AMP-activated protein kinase subunit gamma-1 AMPK gamma1 AMPK subunit gamma-1 AMPKg
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 F1\|15 54.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_016781.2	NP_058061.2	5'-AMP-activated protein kinase subunit gamma-1
XM_011245520.1	XP_011243822.1	5'-AMP-activated protein kinase subunit gamma-1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKAG1	NP_001029999.1
Caenorhabditis elegans (roundworm)	aakg-1	NP_499637.2
Oryza sativa (rice)	Os04g0401300	NP_001052687.2
Xenopus tropicalis (tropical clawed frog)	prkag2	NP_001096506.1
Mus musculus (house mouse)	Prkag1	NP_058061.2
Homo sapiens (human)	PRKAG1	NP_001193638.1
Canis lupus familiaris (dog)	PRKAG1	XP_543685.2
Pan troglodytes (chimpanzee)	PRKAG1	XP_003313774.1
	PRKAG1	XP_001105473.1
Rattus norvegicus (Norway rat)	Prkag1	NP_037142.1
Danio rerio (zebrafish)	prkag1	NP_998326.1
Bos taurus (cattle)	PRKAG1	NP_777011.2

Gene Ontology
Bibliography

Related articles in PubMed

AMPK Re-Activation Suppresses Hepatic Steatosis but its Downregulation Does Not Promote Fatty Liver Development.
Boudaba N, Marion A, Huet C, Pierre R, Viollet B, Foretz M
EBioMedicine(2018 Jan)

Liver-Specific Activation of AMPK Prevents Steatosis on a High-Fructose Diet.
Woods A, Williams JR, Muckett PJ, Mayer FV, Liljevald M, Bohlooly-Y M, Carling D
Cell reports18(13)3043-3051(2017 Mar)

Benzimidazole derivative small-molecule 991 enhances AMPK activity and glucose uptake induced by AICAR or contraction in skeletal muscle.
Bultot L, Jensen TE, Lai YC, Madsen AL, Collodet C, Kviklyte S, Deak M, Yavari A, Foretz M, Ghaffari S, Bellahcene M, Ashrafian H, Rider MH, Richter EA, Sakamoto K
American journal of physiology. Endocrinology and metabolism311(4)E706-E719(2016 Oct)

Skeletal muscle AMP-activated protein kinase ?1(H151R) overexpression enhances whole body energy homeostasis and insulin sensitivity.
Sch?nke M, Myers MG Jr, Zierath JR, Bj?rnholm M
American journal of physiology. Endocrinology and metabolism309(7)E679-90(2015 Oct)

Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators.
Dopie J, Rajakyl? EK, Joensuu MS, Huet G, Ferrantelli E, Xie T, J??linoja H, Jokitalo E, Vartiainen MK
Journal of cell science128(13)2388-400(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results emphasize the clinical relevance of activating AMPK in the liver to combat non-alcoholic fatty liver disease.
Title: Liver-Specific Activation of AMPK Prevents Steatosis on a High-Fructose Diet.

Transgenic activation of skeletal muscle AMPKgamma1 in this model plays an important sex-specific role in skeletal muscle metabolism and whole body energy homeostasis.
Title: Skeletal muscle AMP-activated protein kinase ?1(H151R) overexpression enhances whole body energy homeostasis and insulin sensitivity.

Data indicate that a diet high in iron improves glucose tolerance by activating AMPK through mechanisms that include deacetylation.
Title: Iron regulates glucose homeostasis in liver and muscle via AMP-activated protein kinase in mice.

Results demonstrate that AMPKgamma1 subunit is required for the maintenance of erythrocyte membrane elasticity.
Title: The AMPK?1 subunit plays an essential role in erythrocyte membrane elasticity, and its genetic inactivation induces splenomegaly and anemia.

AMPK is not necessary for exercise training-induced increases in mitochondrial markers, but it is essential for fiber type IIb to IIa/x transformation and increases in hexokinase II protein[AMP-activated protein kinase gamma1]
Title: Skeletal muscle adaptation to exercise training: AMP-activated protein kinase mediates muscle fiber type shift.

The following Prkag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu68490	XM_011245520.1 Latest version!	Mus musculus protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu10571	NM_016781.2 Latest version!	Mus musculus protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkag1 ( XM_011245520.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243822.1 Mus musculus protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu68490
Clone ID Related Accession (Same CDS sequence)	XM_011245520.1
Accession Version	XM_011245520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCTC ATCGCTGCTA TGACCTAATT CCCACAAGTT CCAAGTTGGT GGTATTTGAC 
ACTTCGCTAC AGGTAAAGAA AGCCTTTTTT GCCCTGGTGA CCAATGGTGT TCGTGCCGCC 
CCTTTGTGGG ACAGTAAGAA GCAGAGTTTT GTGGGCATGC TGACCATCAC CGACTTCATC 
AACATTTTGC ACCGATACTA TAAGTCAGCC CTGGTGCAGA TCTACGAACT GGAGGAGCAC 
AAGATAGAGA CGTGGAGAGA GGTGTACCTG CAGGACTCCT TTAAGCCACT TGTCTGCATC 
TCTCCAAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAATAA GATCCACAGG 
CTCCCAGTTA TCGACCCAGA GTCAGGCAAC ACCTTGTACA TCCTTACTCA CAAGCGGATC 
CTCAAGTTCC TCAAGTTGTT TATCACCGAG TTCCCCAAGC CGGAATTCAT GTCTAAGTCT 
CTCCAAGAGC TGCAGATTGG CACCTATGCC AATATTGCCA TGGTCCGTAC TACCACGCCT 
GTCTACGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CCGCCTTACC TGTAGTGGAT 
GAGAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TGATCAATTT GGCAGCCGAA 
AAGACCTACA ACAACCTAGA TGTGTCTGTG ACAAAAGCCC TGCAGCATCG GTCCCACTAC 
TTTGAGGGTG TTCTCAAATG CTACCTGCAT GAGACTCTGG AAACCATCAT CAATAGGCTG 
GTGGAGGCAG AGGTTCACCG TCTGGTGGTG GTGGATGAAC ACGACGTGGT CAAGGGCATC 
GTTTCGCTGT CTGACATCTT ACAGGCTCTG GTGCTCACGG GTGGAGAGAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243822.1
CDS	212..1111
Translation	MKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLQELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDEHDVVKGIVSLSDILQALVLTGGEKKP

Target ORF information:

RefSeq Version	XM_011245520.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245520.1

ORF Insert Sequence:

ATGAAGTCTC ATCGCTGCTA TGACCTAATT CCCACAAGTT CCAAGTTGGT GGTATTTGAC 
ACTTCGCTAC AGGTAAAGAA AGCCTTTTTT GCCCTGGTGA CCAATGGTGT TCGTGCCGCC 
CCTTTGTGGG ACAGTAAGAA GCAGAGTTTT GTGGGCATGC TGACCATCAC CGACTTCATC 
AACATTTTGC ACCGATACTA TAAGTCAGCC CTGGTGCAGA TCTACGAACT GGAGGAGCAC 
AAGATAGAGA CGTGGAGAGA GGTGTACCTG CAGGACTCCT TTAAGCCACT TGTCTGCATC 
TCTCCAAATG CCAGCTTGTT TGATGCTGTC TCTTCATTAA TTCGAAATAA GATCCACAGG 
CTCCCAGTTA TCGACCCAGA GTCAGGCAAC ACCTTGTACA TCCTTACTCA CAAGCGGATC 
CTCAAGTTCC TCAAGTTGTT TATCACCGAG TTCCCCAAGC CGGAATTCAT GTCTAAGTCT 
CTCCAAGAGC TGCAGATTGG CACCTATGCC AATATTGCCA TGGTCCGTAC TACCACGCCT 
GTCTACGTGG CTCTGGGCAT CTTTGTACAG CACCGAGTCT CCGCCTTACC TGTAGTGGAT 
GAGAAAGGGC GTGTGGTGGA CATCTACTCC AAGTTTGATG TGATCAATTT GGCAGCCGAA 
AAGACCTACA ACAACCTAGA TGTGTCTGTG ACAAAAGCCC TGCAGCATCG GTCCCACTAC 
TTTGAGGGTG TTCTCAAATG CTACCTGCAT GAGACTCTGG AAACCATCAT CAATAGGCTG 
GTGGAGGCAG AGGTTCACCG TCTGGTGGTG GTGGATGAAC ACGACGTGGT CAAGGGCATC 
GTTTCGCTGT CTGACATCTT ACAGGCTCTG GTGCTCACGG GTGGAGAGAA GAAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkag1 ( NM_016781.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058061.2 Mus musculus protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1), mRNA.

Price & Availability↑

CloneID	OMu10571
Clone ID Related Accession (Same CDS sequence)	NM_016781.2
Accession Version	NM_016781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	5'-AMP-activated protein kinase subunit gamma-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ963077.1 and BC086660.1. On Jan 25, 2007 this sequence version replaced NM_016781.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086660.1, AK138324.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCGG TTGCTGCAGA GAGCTCGCCA GCTCTAGAGA ATGAACACTT TCAAGAGACC 
CCGGAATCAA ACAATAGTGT GTATACTTCC TTCATGAAGT CTCATCGCTG CTATGACCTA 
ATTCCCACAA GTTCCAAGTT GGTGGTATTT GACACTTCGC TACAGGTAAA GAAAGCCTTT 
TTTGCCCTGG TGACCAATGG TGTTCGTGCC GCCCCTTTGT GGGACAGTAA GAAGCAGAGT 
TTTGTGGGCA TGCTGACCAT CACCGACTTC ATCAACATTT TGCACCGATA CTATAAGTCA 
GCCCTGGTGC AGATCTACGA ACTGGAGGAG CACAAGATAG AGACGTGGAG AGAGGTGTAC 
CTGCAGGACT CCTTTAAGCC ACTTGTCTGC ATCTCTCCAA ATGCCAGCTT GTTTGATGCT 
GTCTCTTCAT TAATTCGAAA TAAGATCCAC AGGCTCCCAG TTATCGACCC AGAGTCAGGC 
AACACCTTGT ACATCCTTAC TCACAAGCGG ATCCTCAAGT TCCTCAAGTT GTTTATCACC 
GAGTTCCCCA AGCCGGAATT CATGTCTAAG TCTCTCCAAG AGCTGCAGAT TGGCACCTAT 
GCCAATATTG CCATGGTCCG TACTACCACG CCTGTCTACG TGGCTCTGGG CATCTTTGTA 
CAGCACCGAG TCTCCGCCTT ACCTGTAGTG GATGAGAAAG GGCGTGTGGT GGACATCTAC 
TCCAAGTTTG ATGTGATCAA TTTGGCAGCC GAAAAGACCT ACAACAACCT AGATGTGTCT 
GTGACAAAAG CCCTGCAGCA TCGGTCCCAC TACTTTGAGG GTGTTCTCAA ATGCTACCTG 
CATGAGACTC TGGAAACCAT CATCAATAGG CTGGTGGAGG CAGAGGTTCA CCGTCTGGTG 
GTGGTGGATG AACACGACGT GGTCAAGGGC ATCGTTTCGC TGTCTGACAT CTTACAGGCT 
CTGGTGCTCA CGGGTGGAGA GAAGAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058061.2
CDS	71..1063
Translation	MESVAAESSPALENEHFQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLQELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDEHDVVKGIVSLSDILQALVLTGGEKKP

Target ORF information:

RefSeq Version	NM_016781.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016781.2

ORF Insert Sequence:

ATGGAGTCGG TTGCTGCAGA GAGCTCGCCA GCTCTAGAGA ATGAACACTT TCAAGAGACC 
CCGGAATCAA ACAATAGTGT GTATACTTCC TTCATGAAGT CTCATCGCTG CTATGACCTA 
ATTCCCACAA GTTCCAAGTT GGTGGTATTT GACACTTCGC TACAGGTAAA GAAAGCCTTT 
TTTGCCCTGG TGACCAATGG TGTTCGTGCC GCCCCTTTGT GGGACAGTAA GAAGCAGAGT 
TTTGTGGGCA TGCTGACCAT CACCGACTTC ATCAACATTT TGCACCGATA CTATAAGTCA 
GCCCTGGTGC AGATCTACGA ACTGGAGGAG CACAAGATAG AGACGTGGAG AGAGGTGTAC 
CTGCAGGACT CCTTTAAGCC ACTTGTCTGC ATCTCTCCAA ATGCCAGCTT GTTTGATGCT 
GTCTCTTCAT TAATTCGAAA TAAGATCCAC AGGCTCCCAG TTATCGACCC AGAGTCAGGC 
AACACCTTGT ACATCCTTAC TCACAAGCGG ATCCTCAAGT TCCTCAAGTT GTTTATCACC 
GAGTTCCCCA AGCCGGAATT CATGTCTAAG TCTCTCCAAG AGCTGCAGAT TGGCACCTAT 
GCCAATATTG CCATGGTCCG TACTACCACG CCTGTCTACG TGGCTCTGGG CATCTTTGTA 
CAGCACCGAG TCTCCGCCTT ACCTGTAGTG GATGAGAAAG GGCGTGTGGT GGACATCTAC 
TCCAAGTTTG ATGTGATCAA TTTGGCAGCC GAAAAGACCT ACAACAACCT AGATGTGTCT 
GTGACAAAAG CCCTGCAGCA TCGGTCCCAC TACTTTGAGG GTGTTCTCAA ATGCTACCTG 
CATGAGACTC TGGAAACCAT CATCAATAGG CTGGTGGAGG CAGAGGTTCA CCGTCTGGTG 
GTGGTGGATG AACACGACGT GGTCAAGGGC ATCGTTTCGC TGTCTGACAT CTTACAGGCT 
CTGGTGCTCA CGGGTGGAGA GAAGAAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AMPK Re-Activation Suppresses Hepatic Steatosis but its Downregulation Does Not Promote Fatty Liver Development.
EBioMedicine(2018 Jan)
Boudaba N,Marion A,Huet C,Pierre R,Viollet B,Foretz M

Liver-Specific Activation of AMPK Prevents Steatosis on a High-Fructose Diet.
Cell reports18(13)3043-3051(2017 Mar)
Woods A,Williams JR,Muckett PJ,Mayer FV,Liljevald M,Bohlooly-Y M,Carling D

Benzimidazole derivative small-molecule 991 enhances AMPK activity and glucose uptake induced by AICAR or contraction in skeletal muscle.
American journal of physiology. Endocrinology and metabolism311(4)E706-E719(2016 Oct)
Bultot L,Jensen TE,Lai YC,Madsen AL,Collodet C,Kviklyte S,Deak M,Yavari A,Foretz M,Ghaffari S,Bellahcene M,Ashrafian H,Rider MH,Richter EA,Sakamoto K

Skeletal muscle AMP-activated protein kinase ?1(H151R) overexpression enhances whole body energy homeostasis and insulin sensitivity.
American journal of physiology. Endocrinology and metabolism309(7)E679-90(2015 Oct)
Sch?nke M,Myers MG Jr,Zierath JR,Bj?rnholm M

Genome-wide RNAi screen for nuclear actin reveals a network of cofilin regulators.
Journal of cell science128(13)2388-400(2015 Jul)
Dopie J,Rajakyl? EK,Joensuu MS,Huet G,Ferrantelli E,Xie T,J??linoja H,Jokitalo E,Vartiainen MK

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Prkag1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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