Emd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emd
Entrez Gene ID	25437
Full Name	emerin
General protein information	Preferred Names emerin Names emerin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37

mRNA and Protein(s)

mRNA	Protein	Name
NM_012948.1	NP_037080.1	emerin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EMD	XP_521335.3
Canis lupus familiaris (dog)	EMD	XP_549369.4
Rattus norvegicus (Norway rat)	Emd	NP_037080.1
Bos taurus (cattle)	EMD	NP_976237.1
Homo sapiens (human)	EMD	NP_000108.1
	EMD	XP_001090376.1
Mus musculus (house mouse)	Emd	NP_031953.1

Gene Ontology
Bibliography

Related articles in PubMed

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.
Piekarowicz K, Machowska M, Dratkiewicz E, Lorek D, Madej-Pilarczyk A, Rzepecki R
Chromosoma126(4)501-517(2017 Aug)

Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient.
Shimojima M, Yuasa S, Motoda C, Yozu G, Nagai T, Ito S, Lachmann M, Kashimura S, Takei M, Kusumoto D, Kunitomi A, Hayashiji N, Seki T, Tohyama S, Hashimoto H, Kodaira M, Egashira T, Hayashi K, Nakanishi C, Sakata K, Yamagishi M, Fukuda K
Scientific reports744312(2017 Mar)

Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application.
Qi YX, Yao QP, Huang K, Shi Q, Zhang P, Wang GL, Han Y, Bao H, Wang L, Li HP, Shen BR, Wang Y, Chien S, Jiang ZL
Proceedings of the National Academy of Sciences of the United States of America113(19)5293-8(2016 May)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Whole exome sequencing identifies a novel EMD mutation in a Chinese family with dilated cardiomyopathy.
Zhang M, Chen J, Si D, Zheng Y, Jiao H, Feng Z, Hu Z, Duan R
BMC medical genetics1577(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings establish the pathogenetic role of emerin and lamin A/C repression in stretch-induced VSMC proliferation and suggest mechanobiological mechanism underlying this process that involves the sequence-specific binding of emerin and lamin A/C to specific transcription factor motifs.
Title: Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application.

We now show that epitope masking in the nucleus is often responsible for failure to detect emerin and lamins in human, rat and pig tissues.
Title: The apparent absence of lamin B1 and emerin in many tissue nuclei is due to epitope masking.

The following Emd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01418	NM_012948.1 Latest version!	Rattus norvegicus emerin (Emd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emd ( NM_012948.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037080.1 Rattus norvegicus emerin (Emd), mRNA.

Price & Availability↑

CloneID	ORa01418
Clone ID Related Accession (Same CDS sequence)	NM_012948.1
Accession Version	NM_012948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	emerin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X98377.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X98377.1, BC059151.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCGCATG GGCCTATTCT GGGCTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG GCTTTCGCCC CCCAGCTCGT CATCGTCTTC ATTCTCCTAT 
CGGTTCTCAG ACTTGGATTC AGCCTCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAG 
GAGGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGCTAT 
TTGACTACCA GGACATACGG GGAGCCCGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCGG 
CCAGGGACCT CACTTGTAGA TGCTGATGAT ACCTTCCATC ACCAGGTGCG TGATGACATT 
TTCTCTTCTT CAGAAGAGGA AGGCAAGGAT AGGGAACGCC CCATCTATGG CCGAGACAGT 
GCCTACCAGA GCATCGCAGA GTACCGCCCC ATTTCCAATG TCTCCAGGAG CTCCCTGGGC 
TTATCATATT ATCCTAGATC CTCCACCTCT TCTGTGTCCT CCTCTTCATC TTCTCCCTCT 
TCATGGCTTA CCCGACGTGC CATTCGACCA GAAAAGCAGG CCCCTACTGC TGCTCTGGGC 
CAGGATCGAC AGGTCCCACT CTGGGGCCAG CTGTTACTCT TCCTGGCCTT TGCTACCTTT 
CTGCTCTTTG TTTACTATTC CATACAAGCT CAAGAAGGCA ACCCCTTCTG GATGGATCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037080.1
CDS	113..895
Translation	MDDYAVLSDTELAAVLRQYNIPHGPILGSTRKLYEKKIFEYETQRRRLSPPSSSSSSFSYRFSDLDSASVDSDMYDLPKKEDALLYQSKDYNDDYYEESYLTTRTYGEPESVGMSKSFRRPGTSLVDADDTFHHQVRDDIFSSSEEEGKDRERPIYGRDSAYQSIAEYRPISNVSRSSLGLSYYPRSSTSSVSSSSSSPSSWLTRRAIRPEKQAPTAALGQDRQVPLWGQLLLFLAFATFLLFVYYSIQAQEGNPFWMDP

Target ORF information:

RefSeq Version	NM_012948.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus emerin (Emd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012948.1

ORF Insert Sequence:

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCGCATG GGCCTATTCT GGGCTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG GCTTTCGCCC CCCAGCTCGT CATCGTCTTC ATTCTCCTAT 
CGGTTCTCAG ACTTGGATTC AGCCTCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAG 
GAGGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGCTAT 
TTGACTACCA GGACATACGG GGAGCCCGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCGG 
CCAGGGACCT CACTTGTAGA TGCTGATGAT ACCTTCCATC ACCAGGTGCG TGATGACATT 
TTCTCTTCTT CAGAAGAGGA AGGCAAGGAT AGGGAACGCC CCATCTATGG CCGAGACAGT 
GCCTACCAGA GCATCGCAGA GTACCGCCCC ATTTCCAATG TCTCCAGGAG CTCCCTGGGC 
TTATCATATT ATCCTAGATC CTCCACCTCT TCTGTGTCCT CCTCTTCATC TTCTCCCTCT 
TCATGGCTTA CCCGACGTGC CATTCGACCA GAAAAGCAGG CCCCTACTGC TGCTCTGGGC 
CAGGATCGAC AGGTCCCACT CTGGGGCCAG CTGTTACTCT TCCTGGCCTT TGCTACCTTT 
CTGCTCTTTG TTTACTATTC CATACAAGCT CAAGAAGGCA ACCCCTTCTG GATGGATCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.
Chromosoma126(4)501-517(2017 Aug)
Piekarowicz K,Machowska M,Dratkiewicz E,Lorek D,Madej-Pilarczyk A,Rzepecki R

Emerin plays a crucial role in nuclear invagination and in the nuclear calcium transient.
Scientific reports744312(2017 Mar)
Shimojima M,Yuasa S,Motoda C,Yozu G,Nagai T,Ito S,Lachmann M,Kashimura S,Takei M,Kusumoto D,Kunitomi A,Hayashiji N,Seki T,Tohyama S,Hashimoto H,Kodaira M,Egashira T,Hayashi K,Nakanishi C,Sakata K,Yamagishi M,Fukuda K

Nuclear envelope proteins modulate proliferation of vascular smooth muscle cells during cyclic stretch application.
Proceedings of the National Academy of Sciences of the United States of America113(19)5293-8(2016 May)
Qi YX,Yao QP,Huang K,Shi Q,Zhang P,Wang GL,Han Y,Bao H,Wang L,Li HP,Shen BR,Wang Y,Chien S,Jiang ZL

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Whole exome sequencing identifies a novel EMD mutation in a Chinese family with dilated cardiomyopathy.
BMC medical genetics1577(2014 Jul)
Zhang M,Chen J,Si D,Zheng Y,Jiao H,Feng Z,Hu Z,Duan R

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Emd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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