Emd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Emd
Entrez Gene ID	13726
Full Name	emerin
Synonyms	AW550900,Sta
General protein information	Preferred Names emerin Names emerin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A7.3\|X 37.92 cM
Summary	This gene is the founding member of the LEM (Lap2beta, Emerin, and Man1) domain gene family. It encodes an integral membrane protein of the inner nuclear membrane. This gene may be involved in transcriptional regulation and genomic organization. In humans, mutations in this gene cause a class of diseases called laminopathies, which includes Emery-Dreifuss muscular dystrophy (EDMD). [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247521.2	XP_011245823.1	emerin isoform X1
NM_007927.3	NP_031953.1	emerin
XM_011247521.3	XP_011245823.1	emerin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EMD	XP_521335.3
Canis lupus familiaris (dog)	EMD	XP_549369.4
Rattus norvegicus (Norway rat)	Emd	NP_037080.1
Bos taurus (cattle)	EMD	NP_976237.1
Homo sapiens (human)	EMD	NP_000108.1
	EMD	XP_001090376.1
Mus musculus (house mouse)	Emd	NP_031953.1

Gene Ontology
Bibliography

Related articles in PubMed

Nesprin 1?2 is essential for mouse postnatal viability and nuclear positioning in skeletal muscle.
Stroud MJ, Feng W, Zhang J, Veevers J, Fang X, Gerace L, Chen J
The Journal of cell biology216(7)1915-1924(2017 Jul)

Substrate stiffness-dependent regulation of the SRF-Mkl1 co-activator complex requires the inner nuclear membrane protein Emerin.
Willer MK, Carroll CW
Journal of cell science130(13)2111-2118(2017 Jul)

Developmental activities of the complement pathway in migrating neurons.
Gorelik A, Sapir T, Haffner-Krausz R, Olender T, Woodruff TM, Reiner O
Nature communications815096(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Emerin-null progenitors were delayed in their cell cycle exit, had decreased myosin heavy chain (MyHC) expression and formed fewer myotubes. Emerin binds to and activates histone deacetylase 3 (HDAC3).
Title: MAPK signaling pathways and HDAC3 activity are disrupted during differentiation of emerin-null myogenic progenitor cells.

changes in nuclear size and shape, which are mediated by nuclear envelope structural proteins lamin A/C and/or emerin, also impact gene regulation and lineage differentiation in early embryos.
Title: Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.

Results suggest that emerin protein is an essential component of the cellular apparatus constraining and fine-tuning Wnt/b-catenin signaling in the heart providing tight control of cardiomyocyte numbers.
Title: Attenuation of Wnt/?-catenin activity reverses enhanced generation of cardiomyocytes and cardiac defects caused by the loss of emerin.

Interactions between HDAC3 and Emerin mediate the interaction of myogenic regulatory loci with the nuclear lamina.
Title: Emerin and histone deacetylase 3 (HDAC3) cooperatively regulate expression and nuclear positions of MyoD, Myf5, and Pax7 genes during myogenesis.

emerin functions with myosin IIB to polarize actin flow and nuclear movement in fibroblasts, suggesting a novel function for the nuclear envelope in organizing directional actin flow and cytoplasmic polarity.
Title: Emerin organizes actin flow for nuclear movement and centrosome orientation in migrating fibroblasts.

The following Emd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Emd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01055	NM_007927.3 Latest version!	Mus musculus emerin (Emd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu67612	XM_011247521.3 Latest version!	Mus musculus emerin (Emd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu67612	XM_011247521.2	Mus musculus emerin (Emd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Emd ( XM_011247521.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245823.1 Mus musculus emerin (Emd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67612
Clone ID Related Accession (Same CDS sequence)	XM_011247521.2 , XM_011247521.3
Accession Version	XM_011247521.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	emerin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCTCATG GGCCTATTGT GGGTTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG ACTCTTACCC CCCAACTCGT CATCTTCTTC ATTCTCCTAT 
CAGTTCTCAG ACTTGGATTC AGCCGCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAA 
GAAGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGTTAT 
TTGACCACCA AGACATACGG GGAGCCTGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCAG 
CCGGGGACCT CACTTGTAGA TGCTGATACC TTCCATCACC AGGTGCGTGA TGACATTTTC 
TCTTCTTTAG AAGAGGAAGG CAAGGATAGC ATCCATGTCT TACAGCTCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245823.1
CDS	281..757
Translation	MDDYAVLSDTELAAVLRQYNIPHGPIVGSTRKLYEKKIFEYETQRRRLLPPNSSSSSFSYQFSDLDSAAVDSDMYDLPKKEDALLYQSKDYNDDYYEESYLTTKTYGEPESVGMSKSFRQPGTSLVDADTFHHQVRDDIFSSLEEEGKDSIHVLQLQY

Target ORF information:

RefSeq Version	XM_011247521.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus emerin (Emd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247521.2

ORF Insert Sequence:

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCTCATG GGCCTATTGT GGGTTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG ACTCTTACCC CCCAACTCGT CATCTTCTTC ATTCTCCTAT 
CAGTTCTCAG ACTTGGATTC AGCCGCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAA 
GAAGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGTTAT 
TTGACCACCA AGACATACGG GGAGCCTGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCAG 
CCGGGGACCT CACTTGTAGA TGCTGATACC TTCCATCACC AGGTGCGTGA TGACATTTTC 
TCTTCTTTAG AAGAGGAAGG CAAGGATAGC ATCCATGTCT TACAGCTCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emd ( NM_007927.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031953.1 Mus musculus emerin (Emd), mRNA.

Price & Availability↑

CloneID	OMu01055
Clone ID Related Accession (Same CDS sequence)	NM_007927.3
Accession Version	NM_007927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	emerin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY260524.1, AK160531.1, AL807376.4 and CN694597.1. On Apr 20, 2013 this sequence version replaced NM_007927.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U73902.1, AK146361.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCTCATG GGCCTATTGT GGGTTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG ACTCTTACCC CCCAACTCGT CATCTTCTTC ATTCTCCTAT 
CAGTTCTCAG ACTTGGATTC AGCCGCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAA 
GAAGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGTTAT 
TTGACCACCA AGACATACGG GGAGCCTGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCAG 
CCGGGGACCT CACTTGTAGA TGCTGATACC TTCCATCACC AGGTGCGTGA TGACATTTTC 
TCTTCTTTAG AAGAGGAAGG CAAGGATAGG GAGCGCCTCA TCTATGGCCA AGACAGTGCC 
TACCAGAGCA TCGCACACTA CCGCCCCATT TCCAATGTCT CCAGGAGCTC TCTGGGCTTA 
TCATATTATC CTACATCCTC CACCTCTTCT GTGTCCTCCT CTTCATCTTC TCCCTCTTCA 
TGGCTTACCC GACGTGCCAT TCGGCCAGAG AAGCAGGCCC CTGCTGCTGC TCTGGGCCAG 
GATCGACAGG TCCCACTCTG GGGCCAGCTG TTACTCTTCC TGGTCTTTGC TGCCTTTCTG 
CTCTTTGTTT ACTATTCCAT ACAAGCTGAA GAAGGCAACC CCTTCTGGAT GGATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031953.1
CDS	266..1045
Translation	MDDYAVLSDTELAAVLRQYNIPHGPIVGSTRKLYEKKIFEYETQRRRLLPPNSSSSSFSYQFSDLDSAAVDSDMYDLPKKEDALLYQSKDYNDDYYEESYLTTKTYGEPESVGMSKSFRQPGTSLVDADTFHHQVRDDIFSSLEEEGKDRERLIYGQDSAYQSIAHYRPISNVSRSSLGLSYYPTSSTSSVSSSSSSPSSWLTRRAIRPEKQAPAAALGQDRQVPLWGQLLLFLVFAAFLLFVYYSIQAEEGNPFWMDP

Target ORF information:

RefSeq Version	NM_007927.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus emerin (Emd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007927.3

ORF Insert Sequence:

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCTCATG GGCCTATTGT GGGTTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG ACTCTTACCC CCCAACTCGT CATCTTCTTC ATTCTCCTAT 
CAGTTCTCAG ACTTGGATTC AGCCGCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAA 
GAAGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGTTAT 
TTGACCACCA AGACATACGG GGAGCCTGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCAG 
CCGGGGACCT CACTTGTAGA TGCTGATACC TTCCATCACC AGGTGCGTGA TGACATTTTC 
TCTTCTTTAG AAGAGGAAGG CAAGGATAGG GAGCGCCTCA TCTATGGCCA AGACAGTGCC 
TACCAGAGCA TCGCACACTA CCGCCCCATT TCCAATGTCT CCAGGAGCTC TCTGGGCTTA 
TCATATTATC CTACATCCTC CACCTCTTCT GTGTCCTCCT CTTCATCTTC TCCCTCTTCA 
TGGCTTACCC GACGTGCCAT TCGGCCAGAG AAGCAGGCCC CTGCTGCTGC TCTGGGCCAG 
GATCGACAGG TCCCACTCTG GGGCCAGCTG TTACTCTTCC TGGTCTTTGC TGCCTTTCTG 
CTCTTTGTTT ACTATTCCAT ACAAGCTGAA GAAGGCAACC CCTTCTGGAT GGATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Emd ( XM_011247521.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245823.1 Mus musculus emerin (Emd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu67612
Clone ID Related Accession (Same CDS sequence)	XM_011247521.2 , XM_011247521.3
Accession Version	XM_011247521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	emerin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCTCATG GGCCTATTGT GGGTTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG ACTCTTACCC CCCAACTCGT CATCTTCTTC ATTCTCCTAT 
CAGTTCTCAG ACTTGGATTC AGCCGCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAA 
GAAGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGTTAT 
TTGACCACCA AGACATACGG GGAGCCTGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCAG 
CCGGGGACCT CACTTGTAGA TGCTGATACC TTCCATCACC AGGTGCGTGA TGACATTTTC 
TCTTCTTTAG AAGAGGAAGG CAAGGATAGC ATCCATGTCT TACAGCTCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245823.1
CDS	279..755
Translation	MDDYAVLSDTELAAVLRQYNIPHGPIVGSTRKLYEKKIFEYETQRRRLLPPNSSSSSFSYQFSDLDSAAVDSDMYDLPKKEDALLYQSKDYNDDYYEESYLTTKTYGEPESVGMSKSFRQPGTSLVDADTFHHQVRDDIFSSLEEEGKDSIHVLQLQY

Target ORF information:

RefSeq Version	XM_011247521.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus emerin (Emd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247521.3

ORF Insert Sequence:

ATGGACGACT ATGCGGTTTT GTCCGATACT GAGCTGGCCG CAGTGCTACG CCAGTACAAC 
ATCCCTCATG GGCCTATTGT GGGTTCCACT CGCAAGCTCT ACGAAAAGAA AATCTTCGAG 
TACGAGACCC AGAGAAGGAG ACTCTTACCC CCCAACTCGT CATCTTCTTC ATTCTCCTAT 
CAGTTCTCAG ACTTGGATTC AGCCGCCGTG GACTCAGACA TGTATGATCT GCCCAAAAAA 
GAAGACGCCT TACTTTACCA GAGCAAGGAC TATAATGATG ACTACTATGA GGAGAGTTAT 
TTGACCACCA AGACATACGG GGAGCCTGAG TCTGTGGGCA TGTCCAAGAG CTTCCGCCAG 
CCGGGGACCT CACTTGTAGA TGCTGATACC TTCCATCACC AGGTGCGTGA TGACATTTTC 
TCTTCTTTAG AAGAGGAAGG CAAGGATAGC ATCCATGTCT TACAGCTCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nesprin 1?2 is essential for mouse postnatal viability and nuclear positioning in skeletal muscle.
The Journal of cell biology216(7)1915-1924(2017 Jul)
Stroud MJ,Feng W,Zhang J,Veevers J,Fang X,Gerace L,Chen J

Substrate stiffness-dependent regulation of the SRF-Mkl1 co-activator complex requires the inner nuclear membrane protein Emerin.
Journal of cell science130(13)2111-2118(2017 Jul)
Willer MK,Carroll CW

Developmental activities of the complement pathway in migrating neurons.
Nature communications815096(2017 May)
Gorelik A,Sapir T,Haffner-Krausz R,Olender T,Woodruff TM,Reiner O

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Emd cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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