Ninj1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ninj1
Entrez Gene ID	25338
Full Name	ninjurin 1
General protein information	Preferred Names ninjurin 1 Names ninjurin-1 nerve injury-induced protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	adhesion molecule; promotes neurite extension of dorsal root ganglion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012867.1	NP_036999.1	ninjurin-1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NINJ1	XP_853215.2
Danio rerio (zebrafish)	LOC100150223	XP_001919024.1
Xenopus tropicalis (tropical clawed frog)	ninj1	NP_001008021.2
	NINJ1	XP_001108521.2
Bos taurus (cattle)	NINJ1	NP_001033171.1
Homo sapiens (human)	NINJ1	NP_004139.2
Gallus gallus (chicken)	NINJ1	NP_001264806.1
Pan troglodytes (chimpanzee)	NINJ1	XP_003312226.1
Mus musculus (house mouse)	Ninj1	NP_038638.1
Rattus norvegicus (Norway rat)	Ninj1	NP_036999.1

Gene Ontology
Bibliography

Related articles in PubMed

Ninjurin1 mediates macrophage-induced programmed cell death during early ocular development.
Lee HJ, Ahn BJ, Shin MW, Jeong JW, Kim JH, Kim KW
Cell death and differentiation16(10)1395-407(2009 Oct)

Ninjurin1 is expressed in myeloid cells and mediates endothelium adhesion in the brains of EAE rats.
Ahn BJ, Lee HJ, Shin MW, Choi JH, Jeong JW, Kim KW
Biochemical and biophysical research communications387(2)321-5(2009 Sep)

Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth.
Araki T, Milbrandt J
Neuron17(2)353-61(1996 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ninj1 may mediate the entry of myeloid cells into the CNS in normal and EAE brains, and it is a potential therapeutic target for regulating myeloid cell trafficking across the blood-brain barrier in CNS immune processes.
Title: Ninjurin1 is expressed in myeloid cells and mediates endothelium adhesion in the brains of EAE rats.

The following Ninj1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ninj1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01161	NM_012867.1 Latest version!	Rattus norvegicus ninjurin 1 (Ninj1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ninj1 ( NM_012867.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036999.1 Rattus norvegicus ninjurin 1 (Ninj1), mRNA.

Price & Availability↑

CloneID	ORa01161
Clone ID Related Accession (Same CDS sequence)	NM_012867.1
Accession Version	NM_012867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	ninjurin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U72660.1. Sequence Note: This sequence has been modified as follows: removed 22 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: U72660.1, FQ219073.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATCCCG GCACCGAGGA GTACGAGCTC AACGGCGACC TGCGCCCTGG CTCTCCCGGT 
TCCCCCGACG CCTCGCCACC CCGCTGGGGT TTGAGGAACC GGCCCATCAA TGTAAACCAT 
TACGCCAACA AGAAGAGCGC CGCGGAGAGC ATGCTGGACA TCGCACTGCT CATGGCCAAC 
GCGTCGCAGC TCAAGGCCGT GGTGGAGCAG GGCAATGAGT TCGCCTTCTT CGTGCCCCTC 
GTGGTGCTCA TCTCCATCTC TCTCGTGCTG CAGATCGGAG TGGGCGTGCT GCTCATCTTC 
CTGGTCAAGT ATGACCTCAA CAACCCAGCC AAGCACGCCA AGCTGGACTT CCTTAACAAC 
CTGGCCACGG GGCTGGTTTT CATCATCGTG GTGGTCAACA TCTTCATTAC GGCCTTTGGA 
GTCCAGAAGC CTGTCATGGA CGTGGCACCC CGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036999.1
CDS	3..461
Translation	MDPGTEEYELNGDLRPGSPGSPDASPPRWGLRNRPINVNHYANKKSAAESMLDIALLMANASQLKAVVEQGNEFAFFVPLVVLISISLVLQIGVGVLLIFLVKYDLNNPAKHAKLDFLNNLATGLVFIIVVVNIFITAFGVQKPVMDVAPRQ

Target ORF information:

RefSeq Version	NM_012867.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ninjurin 1 (Ninj1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012867.1

ORF Insert Sequence:

ATGGATCCCG GCACCGAGGA GTACGAGCTC AACGGCGACC TGCGCCCTGG CTCTCCCGGT 
TCCCCCGACG CCTCGCCACC CCGCTGGGGT TTGAGGAACC GGCCCATCAA TGTAAACCAT 
TACGCCAACA AGAAGAGCGC CGCGGAGAGC ATGCTGGACA TCGCACTGCT CATGGCCAAC 
GCGTCGCAGC TCAAGGCCGT GGTGGAGCAG GGCAATGAGT TCGCCTTCTT CGTGCCCCTC 
GTGGTGCTCA TCTCCATCTC TCTCGTGCTG CAGATCGGAG TGGGCGTGCT GCTCATCTTC 
CTGGTCAAGT ATGACCTCAA CAACCCAGCC AAGCACGCCA AGCTGGACTT CCTTAACAAC 
CTGGCCACGG GGCTGGTTTT CATCATCGTG GTGGTCAACA TCTTCATTAC GGCCTTTGGA 
GTCCAGAAGC CTGTCATGGA CGTGGCACCC CGGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ninjurin1 mediates macrophage-induced programmed cell death during early ocular development.
Cell death and differentiation16(10)1395-407(2009 Oct)
Lee HJ,Ahn BJ,Shin MW,Jeong JW,Kim JH,Kim KW

Ninjurin1 is expressed in myeloid cells and mediates endothelium adhesion in the brains of EAE rats.
Biochemical and biophysical research communications387(2)321-5(2009 Sep)
Ahn BJ,Lee HJ,Shin MW,Choi JH,Jeong JW,Kim KW

Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth.
Neuron17(2)353-61(1996 Aug)
Araki T,Milbrandt J