gRNAs
The following Scaf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scaf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa02151 | |
| Clone ID Related Accession (Same CDS sequence) | NM_139094.1 | |
| Accession Version | NM_139094.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | SR-related and CTD-associated factor 8 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U49055.1. ##Evidence-Data-START## Transcript exon combination :: U49055.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGGCCG TGAAGACCTT CAACAGCGAG TTGTATTCGC TGAATGACTA TAAGCCACCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_620794.1 |
| CDS | 323..4129 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_139094.1 |
1 | ATGGAGGCCG TGAAGACCTT CAACAGCGAG TTGTATTCGC TGAATGACTA TAAGCCACCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa56383 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006227858.3 | |
| Accession Version | XM_006227858.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | protein SCAF8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227858.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTTCCT TCCCGCCTCC GGGATGCCCA GGCCCGGCTC GCACTCTCGC ATTTCCCAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006227920.2 |
| CDS | 623..5221 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006227858.3 |
1 | ATGGGTTCCT TCCCGCCTCC GGGATGCCCA GGCCCGGCTC GCACTCTCGC ATTTCCCAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl-terminal domain of RNA polymerase II. |
A nuclear matrix protein interacts with the phosphorylated C-terminal domain of RNA polymerase II. |
The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. |
