Grm4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grm4
Entrez Gene ID	24417
Full Name	glutamate metabotropic receptor 4
General protein information	Preferred Names glutamate metabotropic receptor 4 Names metabotropic glutamate receptor 4 glutamate receptor, metabotropic 4 mGluR4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	may act as a metabotropic glutamate receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022666.1	NP_073157.1	metabotropic glutamate receptor 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRM4	NP_000832.1
Canis lupus familiaris (dog)	GRM4	XP_005627283.1
Bos taurus (cattle)	GRM4	XP_003587709.1
Mus musculus (house mouse)	Grm4	NP_001013403.1
Xenopus tropicalis (tropical clawed frog)	LOC100491626	XP_002939519.1
Gallus gallus (chicken)	GRM4	XP_003642793.1
Rattus norvegicus (Norway rat)	Grm4	NP_073157.1
Danio rerio (zebrafish)	grm4	XP_695566.4

Gene Ontology
Bibliography

Related articles in PubMed

Selective and interactive effects of D
Isherwood SN, Robbins TW, Nicholson JR, Dalley JW, Pekcec A
Neuropharmacology123249-260(2017 Sep)

Allosteric control of an asymmetric transduction in a G protein-coupled receptor heterodimer.
Liu J, Zhang Z, Moreno-Delgado D, Dalton JA, Rovira X, Trapero A, Goudet C, Llebaria A, Giraldo J, Yuan Q, Rondard P, Huang S, Liu J, Pin JP
eLife6(2017 Aug)

Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells.
Moreno Delgado D, M?ller TC, Ster J, Giraldo J, Maurel D, Rovira X, Scholler P, Zwier JM, Perroy J, Durroux T, Trinquet E, Prezeau L, Rondard P, Pin JP
eLife6(2017 Jun)

Activation of Type 4 Metabotropic Glutamate Receptor Attenuates Oxidative Stress-Induced Death of Neural Stem Cells with Inhibition of JNK and p38 MAPK Signaling.
Zhang Z, Ma W, Wang L, Gong H, Tian Y, Zhang J, Liu J, Lu H, Chen X, Liu Y
Stem cells and development24(22)2709-22(2015 Nov)

Activation of mGluR4 promotes proliferation of rat neural progenitor cells while mediating activation of ERK1/2 signaling pathway.
Duan Z, Zhang X, Zhu GX, Gao Y, Xue X
Cellular and molecular biology (Noisy-le-Grand, France)59 SupplOL1809-17(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

activation of mGluR4 prevents oxidative stress-induced NSC death and apoptotic-associated protein activities with involvement of inhibiting the JNK and p38 pathways in cell culture.
Title: Activation of Type 4 Metabotropic Glutamate Receptor Attenuates Oxidative Stress-Induced Death of Neural Stem Cells with Inhibition of JNK and p38 MAPK Signaling.

mGluR4 promotes the proliferation of rat neural progenitor cells and cyclin D1 expression through activation of ERK1/2 signaling pathways in vitro.
Title: Activation of mGluR4 promotes proliferation of rat neural progenitor cells while mediating activation of ERK1/2 signaling pathway.

In SCG neurons, mGluR2/4 dimers were not inhibited by the mGluR2-selective NAM (Z)-1-[2-cycloheptyloxy-2-(2,6-dichlorophenyl)ethenyl]-1H-1,2,4-triazole (Ro 64-5229), supporting the two-site model.
Title: Functional and pharmacological characteristics of metabotropic glutamate receptors 2/4 heterodimers.

mGluR4 inhibition of presynaptic Ca2+ influx employs a newly defined signalling pathway, notably that involving the activation of phospholipase C and ultimately protein kinase C
Title: A new signalling pathway for parallel fibre presynaptic type 4 metabotropic glutamate receptors (mGluR4) in the rat cerebellar cortex.

native mGluR4 interacts with several exocytosis proteins such as Munc18-1, synapsins, and syntaxin.
Title: Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum.

The following Grm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06209	NM_022666.1 Latest version!	Rattus norvegicus glutamate metabotropic receptor 4 (Grm4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grm4 ( NM_022666.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073157.1 Rattus norvegicus glutamate metabotropic receptor 4 (Grm4), mRNA.

Price & Availability↑

CloneID	ORa06209
Clone ID Related Accession (Same CDS sequence)	NM_022666.1
Accession Version	NM_022666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M90518.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M90518.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGGGA AGGGAGGCTG GGCCTGGTGG TGGGCCCGGC TGCCCCTCTG CCTACTCCTC 
AGCCTTTATG CCCCCTGGGT GCCTTCATCC TTGGGAAAGC CCAAGGGTCA CCCCCACATG 
AACTCTATCC GAATTGACGG GGACATCACA CTGGGAGGCC TGTTTCCCGT CCACGGCCGT 
GGCTCTGAGG GTAAGGCCTG CGGGGAGCTG AAGAAGGAGA AAGGCATCCA CCGCCTGGAG 
GCCATGCTGT TTGCCCTGGA CCGCATCAAC AATGACCCGG ACCTACTGCC CAACATCACG 
TTGGGCGCCC GCATTCTGGA CACCTGCTCG AGGGACACCC ACGCCCTGGA GCAGTCACTG 
ACCTTTGTGC GGGCGCTCAT CGAGAAGGAC GGCACGGAGG TCCGCTGCGG CAGCGGGGGC 
CCGCCCATCA TCACCAAGCC CGAACGAGTG GTGGGTGTCA TTGGAGCTTC GGGGAGCTCC 
GTCTCGATCA TGGTGGCCAA CATCCTCCGC CTCTTCAAGA TCCCTCAGAT CAGCTATGCC 
TCCACGGCCC CTGACTTGAG TGACAACAGC CGCTATGACT TCTTCTCCCG GGTGGTGCCC 
TCAGACACAT ACCAGGCCCA GGCCATGGTG GATATTGTCC GAGCCCTCAA GTGGAACTAT 
GTGTCCACAC TGGCCTCAGA GGGCAGCTAC GGTGAGAGTG GTGTGGAGGC CTTTATCCAG 
AAGTCCCGAG AGAACGGAGG TGTGTGCATT GCCCAGTCGG TGAAGATTCC ACGGGAACCC 
AAGACGGGGG AGTTCGACAA GATCATCAAA CGCCTACTGG AAACATCCAA TGCCAGGGGT 
ATCATCATCT TTGCCAACGA GGATGACATC AGGAGGGTGT TGGAGGCAGC TCGCAGGGCC 
AACCAGACCG GCCACTTCTT TTGGATGGGT TCTGATAGCT GGGGCTCCAA GAGTGCCCCT 
GTGCTGCGCC TTGAGGAGGT GGCCGAGGGC GCAGTCACCA TTCTCCCCAA GAGGATGTCT 
GTTCGAGGGT TCGACCGATA CTTCTCCAGC CGCACGCTGG ACAACAACAG GCGCAACATC 
TGGTTTGCCG AGTTCTGGGA GGACAACTTC CATTGCAAGT TGAGCCGCCA CGCGCTCAAG 
AAGGGAAGCC ACATCAAGAA GTGCACCAAC CGAGAGCGCA TCGGGCAGGA CTCGGCCTAT 
GAGCAGGAGG GGAAGGTGCA GTTCGTGATT GACGCTGTGT ACGCCATGGG CCACGCGCTG 
CACGCCATGC ACCGTGACCT GTGTCCCGGC CGCGTAGGAC TCTGCCCTCG CATGGACCCC 
GTGGATGGCA CCCAGCTGCT TAAGTACATC AGGAACGTCA ACTTCTCAGG CATTGCGGGG 
AACCCTGTAA CCTTCAATGA GAACGGAGAC GCACCGGGGC GCTACGACAT CTACCAGTAC 
CAACTGCGCA ATGGCTCGGC CGAGTACAAG GTCATCGGCT CGTGGACAGA CCACCTGCAC 
CTCAGAATAG AGCGGATGCA GTGGCCAGGG AGTGGCCAGC AGCTGCCGCG CTCCATCTGC 
AGTCTGCCCT GCCAGCCCGG GGAGCGAAAG AAGACTGTGA AGGGCATGGC TTGCTGCTGG 
CACTGCGAGC CCTGCACCGG GTACCAGTAC CAAGTGGACC GCTACACCTG TAAGACCTGC 
CCCTACGACA TGCGGCCCAC AGAGAACCGC ACGAGCTGCC AGCCCATCCC CATCGTCAAG 
TTGGAGTGGG ACTCGCCGTG GGCCGTGCTG CCCCTCTTCC TGGCCGTGGT GGGCATCGCC 
GCCACGCTGT TCGTGGTGGT CACGTTTGTG CGCTACAACG ATACCCCCAT CGTCAAGGCC 
TCGGGCCGGG AGCTGAGCTA CGTGCTGCTG GCGGGCATCT TTCTGTGCTA CGCCACTACC 
TTCCTCATGA TCGCAGAGCC GGACCTGGGG ACCTGTTCGC TCCGCCGCAT CTTCCTAGGG 
CTCGGCATGA GCATCAGCTA CGCGGCCCTG CTGACCAAGA CCAACCGCAT TTACCGCATC 
TTTGAGCAGG GCAAACGGTC GGTCAGTGCC CCGCGTTTCA TCAGCCCGGC CTCGCAGCTG 
GCCATCACCT TCATCCTCAT CTCCCTGCAG CTGCTCGGCA TCTGCGTGTG GTTCGTGGTG 
GACCCCTCCC ACTCGGTGGT GGACTTCCAG GACCAACGGA CACTTGACCC CCGCTTTGCC 
AGGGGCGTGC TCAAGTGCGA CATCTCGGAC CTGTCCCTCA TCTGCCTGCT GGGCTACAGC 
ATGCTGCTGA TGGTCACGTG TACTGTGTAC GCCATCAAGA CCCGAGGCGT GCCCGAGACC 
TTCAACGAGG CCAAGCCCAT CGGCTTCACC ATGTACACCA CCTGCATTGT CTGGCTGGCC 
TTCATCCCCA TCTTTTTTGG CACCTCACAG TCAGCCGACA AGCTGTACAT CCAGACAACC 
ACACTGACGG TCTCCGTGAG TCTGAGCGCT TCAGTGTCCC TGGGGATGCT CTACATGCCC 
AAAGTCTACA TCATCCTCTT CCACCCGGAG CAGAACGTGC CCAAGCGCAA GCGCAGTCTC 
AAAGCCGTGG TCACCGCCGC CACCATGTCC AACAAGTTCA CACAGAAGGG CAACTTCAGG 
CCCAATGGGG AAGCCAAATC AGAGCTGTGT GAGAACCTGG AGACCCCAGC GCTGGCTACC 
AAACAGACCT ACGTCACCTA CACCAACCAT GCCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073157.1
CDS	855..3593
Translation	MSGKGGWAWWWARLPLCLLLSLYAPWVPSSLGKPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKACGELKKEKGIHRLEAMLFALDRINNDPDLLPNITLGARILDTCSRDTHALEQSLTFVRALIEKDGTEVRCGSGGPPIITKPERVVGVIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMVDIVRALKWNYVSTLASEGSYGESGVEAFIQKSRENGGVCIAQSVKIPREPKTGEFDKIIKRLLETSNARGIIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKSAPVLRLEEVAEGAVTILPKRMSVRGFDRYFSSRTLDNNRRNIWFAEFWEDNFHCKLSRHALKKGSHIKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNGSAEYKVIGSWTDHLHLRIERMQWPGSGQQLPRSICSLPCQPGERKKTVKGMACCWHCEPCTGYQYQVDRYTCKTCPYDMRPTENRTSCQPIPIVKLEWDSPWAVLPLFLAVVGIAATLFVVVTFVRYNDTPIVKASGRELSYVLLAGIFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPRFISPASQLAITFILISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCDISDLSLICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSADKLYIQTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSLKAVVTAATMSNKFTQKGNFRPNGEAKSELCENLETPALATKQTYVTYTNHAI

Target ORF information:

RefSeq Version	NM_022666.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate metabotropic receptor 4 (Grm4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022666.1

ORF Insert Sequence:

ATGTCCGGGA AGGGAGGCTG GGCCTGGTGG TGGGCCCGGC TGCCCCTCTG CCTACTCCTC 
AGCCTTTATG CCCCCTGGGT GCCTTCATCC TTGGGAAAGC CCAAGGGTCA CCCCCACATG 
AACTCTATCC GAATTGACGG GGACATCACA CTGGGAGGCC TGTTTCCCGT CCACGGCCGT 
GGCTCTGAGG GTAAGGCCTG CGGGGAGCTG AAGAAGGAGA AAGGCATCCA CCGCCTGGAG 
GCCATGCTGT TTGCCCTGGA CCGCATCAAC AATGACCCGG ACCTACTGCC CAACATCACG 
TTGGGCGCCC GCATTCTGGA CACCTGCTCG AGGGACACCC ACGCCCTGGA GCAGTCACTG 
ACCTTTGTGC GGGCGCTCAT CGAGAAGGAC GGCACGGAGG TCCGCTGCGG CAGCGGGGGC 
CCGCCCATCA TCACCAAGCC CGAACGAGTG GTGGGTGTCA TTGGAGCTTC GGGGAGCTCC 
GTCTCGATCA TGGTGGCCAA CATCCTCCGC CTCTTCAAGA TCCCTCAGAT CAGCTATGCC 
TCCACGGCCC CTGACTTGAG TGACAACAGC CGCTATGACT TCTTCTCCCG GGTGGTGCCC 
TCAGACACAT ACCAGGCCCA GGCCATGGTG GATATTGTCC GAGCCCTCAA GTGGAACTAT 
GTGTCCACAC TGGCCTCAGA GGGCAGCTAC GGTGAGAGTG GTGTGGAGGC CTTTATCCAG 
AAGTCCCGAG AGAACGGAGG TGTGTGCATT GCCCAGTCGG TGAAGATTCC ACGGGAACCC 
AAGACGGGGG AGTTCGACAA GATCATCAAA CGCCTACTGG AAACATCCAA TGCCAGGGGT 
ATCATCATCT TTGCCAACGA GGATGACATC AGGAGGGTGT TGGAGGCAGC TCGCAGGGCC 
AACCAGACCG GCCACTTCTT TTGGATGGGT TCTGATAGCT GGGGCTCCAA GAGTGCCCCT 
GTGCTGCGCC TTGAGGAGGT GGCCGAGGGC GCAGTCACCA TTCTCCCCAA GAGGATGTCT 
GTTCGAGGGT TCGACCGATA CTTCTCCAGC CGCACGCTGG ACAACAACAG GCGCAACATC 
TGGTTTGCCG AGTTCTGGGA GGACAACTTC CATTGCAAGT TGAGCCGCCA CGCGCTCAAG 
AAGGGAAGCC ACATCAAGAA GTGCACCAAC CGAGAGCGCA TCGGGCAGGA CTCGGCCTAT 
GAGCAGGAGG GGAAGGTGCA GTTCGTGATT GACGCTGTGT ACGCCATGGG CCACGCGCTG 
CACGCCATGC ACCGTGACCT GTGTCCCGGC CGCGTAGGAC TCTGCCCTCG CATGGACCCC 
GTGGATGGCA CCCAGCTGCT TAAGTACATC AGGAACGTCA ACTTCTCAGG CATTGCGGGG 
AACCCTGTAA CCTTCAATGA GAACGGAGAC GCACCGGGGC GCTACGACAT CTACCAGTAC 
CAACTGCGCA ATGGCTCGGC CGAGTACAAG GTCATCGGCT CGTGGACAGA CCACCTGCAC 
CTCAGAATAG AGCGGATGCA GTGGCCAGGG AGTGGCCAGC AGCTGCCGCG CTCCATCTGC 
AGTCTGCCCT GCCAGCCCGG GGAGCGAAAG AAGACTGTGA AGGGCATGGC TTGCTGCTGG 
CACTGCGAGC CCTGCACCGG GTACCAGTAC CAAGTGGACC GCTACACCTG TAAGACCTGC 
CCCTACGACA TGCGGCCCAC AGAGAACCGC ACGAGCTGCC AGCCCATCCC CATCGTCAAG 
TTGGAGTGGG ACTCGCCGTG GGCCGTGCTG CCCCTCTTCC TGGCCGTGGT GGGCATCGCC 
GCCACGCTGT TCGTGGTGGT CACGTTTGTG CGCTACAACG ATACCCCCAT CGTCAAGGCC 
TCGGGCCGGG AGCTGAGCTA CGTGCTGCTG GCGGGCATCT TTCTGTGCTA CGCCACTACC 
TTCCTCATGA TCGCAGAGCC GGACCTGGGG ACCTGTTCGC TCCGCCGCAT CTTCCTAGGG 
CTCGGCATGA GCATCAGCTA CGCGGCCCTG CTGACCAAGA CCAACCGCAT TTACCGCATC 
TTTGAGCAGG GCAAACGGTC GGTCAGTGCC CCGCGTTTCA TCAGCCCGGC CTCGCAGCTG 
GCCATCACCT TCATCCTCAT CTCCCTGCAG CTGCTCGGCA TCTGCGTGTG GTTCGTGGTG 
GACCCCTCCC ACTCGGTGGT GGACTTCCAG GACCAACGGA CACTTGACCC CCGCTTTGCC 
AGGGGCGTGC TCAAGTGCGA CATCTCGGAC CTGTCCCTCA TCTGCCTGCT GGGCTACAGC 
ATGCTGCTGA TGGTCACGTG TACTGTGTAC GCCATCAAGA CCCGAGGCGT GCCCGAGACC 
TTCAACGAGG CCAAGCCCAT CGGCTTCACC ATGTACACCA CCTGCATTGT CTGGCTGGCC 
TTCATCCCCA TCTTTTTTGG CACCTCACAG TCAGCCGACA AGCTGTACAT CCAGACAACC 
ACACTGACGG TCTCCGTGAG TCTGAGCGCT TCAGTGTCCC TGGGGATGCT CTACATGCCC 
AAAGTCTACA TCATCCTCTT CCACCCGGAG CAGAACGTGC CCAAGCGCAA GCGCAGTCTC 
AAAGCCGTGG TCACCGCCGC CACCATGTCC AACAAGTTCA CACAGAAGGG CAACTTCAGG 
CCCAATGGGG AAGCCAAATC AGAGCTGTGT GAGAACCTGG AGACCCCAGC GCTGGCTACC 
AAACAGACCT ACGTCACCTA CACCAACCAT GCCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective and interactive effects of D
Neuropharmacology123249-260(2017 Sep)
Isherwood SN,Robbins TW,Nicholson JR,Dalley JW,Pekcec A

Allosteric control of an asymmetric transduction in a G protein-coupled receptor heterodimer.
eLife6(2017 Aug)
Liu J,Zhang Z,Moreno-Delgado D,Dalton JA,Rovira X,Trapero A,Goudet C,Llebaria A,Giraldo J,Yuan Q,Rondard P,Huang S,Liu J,Pin JP

Pharmacological evidence for a metabotropic glutamate receptor heterodimer in neuronal cells.
eLife6(2017 Jun)
Moreno Delgado D,M?ller TC,Ster J,Giraldo J,Maurel D,Rovira X,Scholler P,Zwier JM,Perroy J,Durroux T,Trinquet E,Prezeau L,Rondard P,Pin JP

Activation of Type 4 Metabotropic Glutamate Receptor Attenuates Oxidative Stress-Induced Death of Neural Stem Cells with Inhibition of JNK and p38 MAPK Signaling.
Stem cells and development24(22)2709-22(2015 Nov)
Zhang Z,Ma W,Wang L,Gong H,Tian Y,Zhang J,Liu J,Lu H,Chen X,Liu Y

Activation of mGluR4 promotes proliferation of rat neural progenitor cells while mediating activation of ERK1/2 signaling pathway.
Cellular and molecular biology (Noisy-le-Grand, France)59 SupplOL1809-17(2013 Jan)
Duan Z,Zhang X,Zhu GX,Gao Y,Xue X

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Grm4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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