Mmp13 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmp13
Entrez Gene ID	171052
Full Name	matrix metallopeptidase 13
General protein information	Preferred Names matrix metallopeptidase 13 Names collagenase 3 MMP-13 UMRCASE interstitial collagenase matrix metalloproteinase-13
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q11
Summary	enzyme that degrades collagen type I [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133530.1	NP_598214.1	collagenase 3 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MMP13	XP_001154361.1
Danio rerio (zebrafish)	mmp13b	XP_001345507.1
Gallus gallus (chicken)	MMP13	XP_003640635.1
Xenopus tropicalis (tropical clawed frog)	LOC100490019	XP_002934971.1
Canis lupus familiaris (dog)	MMP13	XP_536598.3
Mus musculus (house mouse)	Mmp13	NP_032633.1
Homo sapiens (human)	MMP13	NP_002418.1
	MMP13	XP_001098996.1
Bos taurus (cattle)	MMP13	NP_776814.1
Rattus norvegicus (Norway rat)	Mmp13	NP_598214.1
Caenorhabditis elegans (roundworm)	CELE_C31B8.8	NP_503790.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of Runx2 phosphorylation sites required for TGF-?1-mediated stimulation of matrix metalloproteinase-13 expression in osteoblastic cells.
Arumugam B, Vairamani M, Partridge NC, Selvamurugan N
Journal of cellular physiology233(2)1082-1094(2018 Feb)

Autophagy Protects Advanced Glycation End Product-Induced Apoptosis and Expression of MMP-3 and MMP-13 in Rat Chondrocytes.
Huang W, Ao P, Li J, Wu T, Xu L, Deng Z, Chen W, Yin C, Cheng X
BioMed research international20176341919(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TGF-beta1 stimulates the phosphorylation of Runx2 at three serine amino acids, and this event is required for MMP-13 expression in osteoblastic cells.
Title: Characterization of Runx2 phosphorylation sites required for TGF-?1-mediated stimulation of matrix metalloproteinase-13 expression in osteoblastic cells.

the up-regulation of MMP genes is mediated through degradation of class IIa histone deacetylases (HDACs) by certain cysteine cathepsins (Cts).
Title: Cathepsin H-Mediated Degradation of HDAC4 for Matrix Metalloproteinase Expression in Hepatic Stellate Cells: Implications of Epigenetic Suppression of Matrix Metalloproteinases in Fibrosis through Stabilization of Class IIa Histone Deacetylases.

autophagy is linked with Advanced Glycation End Product-related pathology in rat chondrocytes and plays a protective role in AGE-induced apoptosis and expression of MMP-3 and MMP-13.
Title: Autophagy Protects Advanced Glycation End Product-Induced Apoptosis and Expression of MMP-3 and MMP-13 in Rat Chondrocytes.

Results show MMP-13 expression increased during liver fibrosis and resolution as well reaching to 40 fold at that time.
Title: Involvement of fibroblast-specific protein 1 (S100A4) and matrix metalloproteinase-13 (MMP-13) in CCl4-induced reversible liver fibrosis.

our data strongly suggest that MMP13 involves VSMCs migration via an Akt and ERK-dependent regulation
Title: Effects of matrix metalloproteinase 13 on vascular smooth muscle cells migration via Akt-ERK dependent pathway.

The following Mmp13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmp13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13330	NM_133530.1 Latest version!	Rattus norvegicus matrix metallopeptidase 13 (Mmp13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmp13 ( NM_133530.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598214.1 Rattus norvegicus matrix metallopeptidase 13 (Mmp13), mRNA.

Price & Availability↑

CloneID	ORa13330
Clone ID Related Accession (Same CDS sequence)	NM_133530.1
Accession Version	NM_133530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	collagenase 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC120947.5. On or before Nov 23, 2008 this sequence version replaced XM_343345.3, XM_001072242.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M60616.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGCATTCAG CTATTCTGGC CACCTTCTTC TTGTTGAGTT GGACTCACTG TTGGTCCCTG 
CCCCTTCCCT ATGGTGATGA TGATGATGAT GACTTGTCTG AGGAAGATCT TGAGTTTGCA 
GAGCACTACT TGAAATCATA CTACCATCCT GTGACTCTTG CGGGAATCCT GAAGAAGTCT 
ACAGTGACCT CTACAGTTGA TAGACTCCGA GAAATGCAGT CTTTCTTTGG CTTAGATGTG 
ACTGGCAAAC TTGATGATCC TACCTTAGAC ATCATGAGAA AACCAAGATG TGGAGTGCCT 
GATGTGGGTG AATACAATGT TTTCCCTCGA ACACTCAAAT GGTCCCAAAC AAACTTAACT 
TACAGAATTG TGAACTACAC CCCTGATATA TCCCATTCTG AAGTGGAGAA GGCCTTCAGA 
AAAGCCTTCA AGGTCTGGTC TGATGTGACA CCTCTGAATT TTACCAGGAT CCATGATGGC 
ACTGCTGATA TCATGATATC TTTTGGAACT AAAGAACATG GTGACTTCTA CCCATTTGAT 
GGGCCTTCTG GTCTTCTGGC ACACGCTTTT CCTCCTGGAC CAAACCTTGG CGGGGACGCC 
CATTTTGATG ATGATGAAAC CTGGACAAGC AGCTCCAAAG GCTACAACTT ATTTATTGTT 
GCTGCCCATG AGCTTGGCCA CTCCCTCGGT CTGGACCACT CCAAGGACCC TGGAGCCCTG 
ATGTTTCCCA TCTATACCTA CACTGGCAAA AGCCATTTCA TGCTCCCAGA TGATGACGTT 
CAAGGAATCC AGTCTCTCTA TGGTCCAGGA GATGAAGACC CCAACCCTAA GCACCCCAAA 
ACACCAGAGA AGTGTGACCC AGCCCTATCC CTTGATGCCA TTACTAGTCT CCGAGGAGAA 
ACCATGATCT TTAAAGACAG ATTCTTCTGG CGTCTGCACC CTCAGCAGGT TGAGCCTGAA 
CTGTTTTTGA CCAAGTCCTT TTGGCCAGAA CTTCCCAACC ATGTGGATGC TGCATACGAG 
CATCCATCCC GAGACCTCAT GTTCATCTTT AGAGGGAGAA AATTCTGGGC CCTGAATGGG 
TATGACATTA TGGAGGGTTA TCCCAGAAAA ATATCTGACC TGGGATTTCC AAAAGAGGTG 
AAAAGGCTCA GTGCTGCGGT TCACTTTGAG GACACGGGGA AGACCCTCTT CTTCTCAGGG 
AACCACGTGT GGAGTTATGA TGATGCTAAC CAGACTATGG ACAAAGACTA TCCCCGCCTC 
ATAGAAGAAG AATTCCCTGG AATTGGCGAC AAAGTAGATG CTGTCTATGA GAAAAATGGC 
TATATCTACT TTTTCAATGG GCCCATACAG TTTGAATACA GTATCTGGAG TAATCGCATT 
GTGAGAGTCA TGCCAACAAA TTCCTTATTG TGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598214.1
CDS	11..1429
Translation	MHSAILATFFLLSWTHCWSLPLPYGDDDDDDLSEEDLEFAEHYLKSYYHPVTLAGILKKSTVTSTVDRLREMQSFFGLDVTGKLDDPTLDIMRKPRCGVPDVGEYNVFPRTLKWSQTNLTYRIVNYTPDISHSEVEKAFRKAFKVWSDVTPLNFTRIHDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNLGGDAHFDDDETWTSSSKGYNLFIVAAHELGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPEKCDPALSLDAITSLRGETMIFKDRFFWRLHPQQVEPELFLTKSFWPELPNHVDAAYEHPSRDLMFIFRGRKFWALNGYDIMEGYPRKISDLGFPKEVKRLSAAVHFEDTGKTLFFSGNHVWSYDDANQTMDKDYPRLIEEEFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPTNSLLWC

Target ORF information:

RefSeq Version	NM_133530.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus matrix metallopeptidase 13 (Mmp13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133530.1

ORF Insert Sequence:

ATGCATTCAG CTATTCTGGC CACCTTCTTC TTGTTGAGTT GGACTCACTG TTGGTCCCTG 
CCCCTTCCCT ATGGTGATGA TGATGATGAT GACTTGTCTG AGGAAGATCT TGAGTTTGCA 
GAGCACTACT TGAAATCATA CTACCATCCT GTGACTCTTG CGGGAATCCT GAAGAAGTCT 
ACAGTGACCT CTACAGTTGA TAGACTCCGA GAAATGCAGT CTTTCTTTGG CTTAGATGTG 
ACTGGCAAAC TTGATGATCC TACCTTAGAC ATCATGAGAA AACCAAGATG TGGAGTGCCT 
GATGTGGGTG AATACAATGT TTTCCCTCGA ACACTCAAAT GGTCCCAAAC AAACTTAACT 
TACAGAATTG TGAACTACAC CCCTGATATA TCCCATTCTG AAGTGGAGAA GGCCTTCAGA 
AAAGCCTTCA AGGTCTGGTC TGATGTGACA CCTCTGAATT TTACCAGGAT CCATGATGGC 
ACTGCTGATA TCATGATATC TTTTGGAACT AAAGAACATG GTGACTTCTA CCCATTTGAT 
GGGCCTTCTG GTCTTCTGGC ACACGCTTTT CCTCCTGGAC CAAACCTTGG CGGGGACGCC 
CATTTTGATG ATGATGAAAC CTGGACAAGC AGCTCCAAAG GCTACAACTT ATTTATTGTT 
GCTGCCCATG AGCTTGGCCA CTCCCTCGGT CTGGACCACT CCAAGGACCC TGGAGCCCTG 
ATGTTTCCCA TCTATACCTA CACTGGCAAA AGCCATTTCA TGCTCCCAGA TGATGACGTT 
CAAGGAATCC AGTCTCTCTA TGGTCCAGGA GATGAAGACC CCAACCCTAA GCACCCCAAA 
ACACCAGAGA AGTGTGACCC AGCCCTATCC CTTGATGCCA TTACTAGTCT CCGAGGAGAA 
ACCATGATCT TTAAAGACAG ATTCTTCTGG CGTCTGCACC CTCAGCAGGT TGAGCCTGAA 
CTGTTTTTGA CCAAGTCCTT TTGGCCAGAA CTTCCCAACC ATGTGGATGC TGCATACGAG 
CATCCATCCC GAGACCTCAT GTTCATCTTT AGAGGGAGAA AATTCTGGGC CCTGAATGGG 
TATGACATTA TGGAGGGTTA TCCCAGAAAA ATATCTGACC TGGGATTTCC AAAAGAGGTG 
AAAAGGCTCA GTGCTGCGGT TCACTTTGAG GACACGGGGA AGACCCTCTT CTTCTCAGGG 
AACCACGTGT GGAGTTATGA TGATGCTAAC CAGACTATGG ACAAAGACTA TCCCCGCCTC 
ATAGAAGAAG AATTCCCTGG AATTGGCGAC AAAGTAGATG CTGTCTATGA GAAAAATGGC 
TATATCTACT TTTTCAATGG GCCCATACAG TTTGAATACA GTATCTGGAG TAATCGCATT 
GTGAGAGTCA TGCCAACAAA TTCCTTATTG TGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of Runx2 phosphorylation sites required for TGF-?1-mediated stimulation of matrix metalloproteinase-13 expression in osteoblastic cells.
Journal of cellular physiology233(2)1082-1094(2018 Feb)
Arumugam B,Vairamani M,Partridge NC,Selvamurugan N

Autophagy Protects Advanced Glycation End Product-Induced Apoptosis and Expression of MMP-3 and MMP-13 in Rat Chondrocytes.
BioMed research international20176341919(2017)
Huang W,Ao P,Li J,Wu T,Xu L,Deng Z,Chen W,Yin C,Cheng X

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Mmp13 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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