Akap5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akap5
Entrez Gene ID	171026
Full Name	A-kinase anchoring protein 5
Synonyms	AKAP150,Akap79,P150
General protein information	Preferred Names A-kinase anchoring protein 5 Names A-kinase anchor protein 5 A kinase (PRKA) anchor protein 5 A-kinase anchor protein 150 kDa AKAP 150 AKAP-5 RII-B-binding protein cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_133515.2	NP_598199.2	A-kinase anchor protein 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Akap5	NP_598199.2
Bos taurus (cattle)	AKAP5	NP_776661.1
Homo sapiens (human)	AKAP5	NP_004848.3
Pan troglodytes (chimpanzee)	AKAP5	XP_001170633.1
	AKAP5	XP_001101989.1
Canis lupus familiaris (dog)	AKAP5	XP_005623550.1
Mus musculus (house mouse)	Akap5	NP_001094941.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of plasma membrane-associated AKAPs for the regulation of cardiac I
Seyler C, Scherer D, K?pple C, Kulzer M, Korkmaz S, Xynogalos P, Thomas D, Kaya Z, Scholz E, Backs J, Karle C, Katus HA, Zitron E
Naunyn-Schmiedeberg's archives of pharmacology390(5)493-503(2017 May)

Peripheral group I metabotropic glutamate receptor activation leads to muscle mechanical hyperalgesia through TRPV1 phosphorylation in the rat.
Chung MK, Lee J, Joseph J, Saloman J, Ro JY
The journal of pain : official journal of the American Pain Society16(1)67-76(2015 Jan)

PKA-GluA1 coupling via AKAP5 controls AMPA receptor phosphorylation and cell-surface targeting during bidirectional homeostatic plasticity.
Diering GH, Gustina AS, Huganir RL
Neuron84(4)790-805(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report coupling of cardiac Kir2.1 and Kir2.2 subunits with the plasma membrane-bound AKAPs 15 and 79. Cardiac membrane-associated AKAPs are a functionally essential part of the regulatory cascade determining IK1 current function.
Title: Role of plasma membrane-associated AKAPs for the regulation of cardiac I

Study shows that mGlu1/5 activation leads to phosphorylation of a specific TRPV1 residue via PKC and AKAP150 in trigeminal sensory neurons and that functional interactions between glutamate receptors and TRPV1 mediate muscle tissue mechanical hyperalgesia
Title: Peripheral group I metabotropic glutamate receptor activation leads to muscle mechanical hyperalgesia through TRPV1 phosphorylation in the rat.

knockdown of Akap5 blocked homeostatic scaling and thus synaptic plasticity.
Title: PKA-GluA1 coupling via AKAP5 controls AMPA receptor phosphorylation and cell-surface targeting during bidirectional homeostatic plasticity.

Cardiac AKAP150 positively responds to hyperglycemia and enhances the efficiency of glucotoxicity signaling through a cPKC/p47(phox)/ROS pathway that induces myocardial dysfunction, cardiomyocyte apoptosis, and oxidative stress.
Title: AKAP150 mobilizes cPKC-dependent cardiac glucotoxicity.

a significant role for the AKAP5 scaffold in signaling and trafficking of the beta1-AR in cardiac myocytes and mammalian cells.
Title: Role of AKAP79/150 protein in ?1-adrenergic receptor trafficking and signaling in mammalian cells.

The following Akap5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akap5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12158	NM_133515.2 Latest version!	Rattus norvegicus A-kinase anchoring protein 5 (Akap5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akap5 ( NM_133515.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598199.2 Rattus norvegicus A-kinase anchoring protein 5 (Akap5), mRNA.

Price & Availability↑

CloneID	ORa12158
Clone ID Related Accession (Same CDS sequence)	NM_133515.2
Accession Version	NM_133515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Rattus norvegicus(Norway rat)
Product	A-kinase anchor protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128637.4. On Aug 3, 2012 this sequence version replaced NM_133515.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U67136.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGAGACCA GCGTTTCTGA GATTCAAATC GAGACTAAGG ATGAGAAGAG ACCAGAAGCA 
GCCAGTCCTC AGAAAGAGAG GCAGGAGAGA AAAACAGCCA CACTGTGCTT CAAGAGAAGG 
AAGAAAGTGA ACAAGAAGAA GGCCAAAGCT GGTTCCAAGA CTGCTGAGGA GACAAAGAAG 
CATGCCCCAG AAGCTGGGGG TTCTGGTCAG CGGCAACCAG CGGGGGCCTG GGCCTCCATT 
AAACGCCTTG TGACACACAG GAAACCGTCC GAGTCTGCAG AGAAGCAGAA GCCATCTGAG 
GCTGAAATGC AGCCTGAGGA TGGAGCTCTT CCTAAGAAGA AGACAAAATC CAAACTTAAG 
ATTCCTTGCA TACGATTCTC AAGAGGGGCA AAGAGAAGTC GTCCTTCCAA ACTCACAGAA 
GATTCAGGCT ATGTCCGAGT CCAGGGAGAG GCAGACGATT TGGAGATAAA AGCCCAGATC 
CAGCCCGATG AGCAGGCAAC CCAGGCTAAG TCCACCCAGG GCCTACAGGA AGATGTGATG 
GTGAGAGATG GTAAGGAGAT CCAAGAATCA CACATAAGCA ACAACGTCAT ATCGGGAGAG 
CATGTGATTG CCATAGAACT GGAATTAGAA AAGGAATCTT CTGCTCTCCG GATGAGAACT 
CCAGGGAGTG AAAAGGAGGC TAAAGTGATT CTGGTAAAGC AGAGTGTTCA GGTACAGCAA 
GCAAGTGTGC TTGAGAATTC AGCAGCAGAC AGCCCTCAGC CAGTGACTTC TACTGCTCCT 
CTCTCACCAG CAACCACACA TCAACTCGGT CTGGAAGAAC CCAGTGACAG CATCCGGGAA 
AGTGCACCAA GTGGAAAAGA TGACGGAAGG AGAAAGACTG CTGCTGAAGA AAAGAAATCA 
GGAGAGACTG CGCTGGGCCA GGCAGAGGAG GCTTCTTCAG TCAGCCAGGC AGACAAAAGT 
GTACTAAGCC AGGCAGAAGA AGCTACAGTG GGCCACACAG AGGAAGCTAC TGTGATCCAG 
GCACAGAGTC AGGCAAAGGA AGGTAAACTG AGCCAGGCAG AGGAAGCTAC AGTGGCCCAG 
GCAAAGGAAA CTGTATTGAG TCAGGCAGAG GAAGTTAAAC TGAGCCAGAT AGAAGAACCT 
GCAATTAGCC AGGCAAAGAA AGCTACAGTA GGACAAGCAA AGGAAGCTAT AGTTAGCCAG 
GCAGAGGAAG CTATAGTTGG TCACACAGAG AAAGCCACCA TGGGTCAGGC AGAGGAAGCT 
ACAGTTGGCC ACATAGAGAA AACCACAGTG GGCCAGGCAG AGGAAGCTAC AGTGGGTCAA 
GCAGAGGAAG CCACAGTGGG TCAAGCAGAG GAAGCCACAG TGGGTCAAGC AGAGGAAGCT 
ACAGTTGGAC AGGCAGAGGA AGCTACAGTT GGTCAGGCAG GGGAAGCTAC AGTTAGCCAC 
ATAGAGAAAA CCACAGTGGG CCAGGCAGAG GAAGCTATAG TTGGTCAGGC AGAGGAAGCC 
ACAGTGGGTC AAGCAGAGGA AGCCACAGTG GGTCAAGCAG AGGAAGCTAC AGTTGGCCAG 
GCAGAGGAAG CCACAGTGGA TCAAGCAGAG GAAGCTACAG TTGGCCAGGC AGAGGAAGCT 
ACAGTTGGTC AGGCAGGGGA AGCTGCAGTT GGCCAGGCAG AGGAAGCTAT AGTTGCTCAG 
GCAGAGGAAG CCACAGTTGG TCAGGCAGGG GAAGCTACAG TTGGCCAAGC AGAGAAAGCC 
ACAGTTGGCC AAGCAGAGGA ACCCATAGTT GGCCAGGCAG AGGAAACTGT ATTGAGGCAT 
GCATCAGATC TGAAAGTAAA TGGAGTTGAT GCAGAGAAAC CAAGATCAGA AGAAAGCAAA 
AGAATGGAGC CAATTGCTAT TATCATAACA GACACTGAAA TCAGTGAATT TGATGTTAAG 
AAATCTAAAA ATGTCCCTAA GCAATTCCTA ATTTCCATGG AAAATGAGCA AGTGGGGGTT 
TTTGCTAATG ATAGTGATTT CGAGGGGAGA ACTTCAGAAC AGTATGAAAC ACTCTTAATA 
GAAACAGCTT CTTCTCTCGT CAAGAATGCT ATCGAGTTGT CTGTAGAACA GCTGGTTAAC 
GAAATGGTTT CTGAGGATAA TCAAATAAAT ACGCTGTTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598199.2
CDS	526..2670
Translation	METSVSEIQIETKDEKRPEAASPQKERQERKTATLCFKRRKKVNKKKAKAGSKTAEETKKHAPEAGGSGQRQPAGAWASIKRLVTHRKPSESAEKQKPSEAEMQPEDGALPKKKTKSKLKIPCIRFSRGAKRSRPSKLTEDSGYVRVQGEADDLEIKAQIQPDEQATQAKSTQGLQEDVMVRDGKEIQESHISNNVISGEHVIAIELELEKESSALRMRTPGSEKEAKVILVKQSVQVQQASVLENSAADSPQPVTSTAPLSPATTHQLGLEEPSDSIRESAPSGKDDGRRKTAAEEKKSGETALGQAEEASSVSQADKSVLSQAEEATVGHTEEATVIQAQSQAKEGKLSQAEEATVAQAKETVLSQAEEVKLSQIEEPAISQAKKATVGQAKEAIVSQAEEAIVGHTEKATMGQAEEATVGHIEKTTVGQAEEATVGQAEEATVGQAEEATVGQAEEATVGQAEEATVGQAGEATVSHIEKTTVGQAEEAIVGQAEEATVGQAEEATVGQAEEATVGQAEEATVDQAEEATVGQAEEATVGQAGEAAVGQAEEAIVAQAEEATVGQAGEATVGQAEKATVGQAEEPIVGQAEETVLRHASDLKVNGVDAEKPRSEESKRMEPIAIIITDTEISEFDVKKSKNVPKQFLISMENEQVGVFANDSDFEGRTSEQYETLLIETASSLVKNAIELSVEQLVNEMVSEDNQINTLFQ

Target ORF information:

RefSeq Version	NM_133515.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus A-kinase anchoring protein 5 (Akap5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133515.2

ORF Insert Sequence:

ATGGAGACCA GCGTTTCTGA GATTCAAATC GAGACTAAGG ATGAGAAGAG ACCAGAAGCA 
GCCAGTCCTC AGAAAGAGAG GCAGGAGAGA AAAACAGCCA CACTGTGCTT CAAGAGAAGG 
AAGAAAGTGA ACAAGAAGAA GGCCAAAGCT GGTTCCAAGA CTGCTGAGGA GACAAAGAAG 
CATGCCCCAG AAGCTGGGGG TTCTGGTCAG CGGCAACCAG CGGGGGCCTG GGCCTCCATT 
AAACGCCTTG TGACACACAG GAAACCGTCC GAGTCTGCAG AGAAGCAGAA GCCATCTGAG 
GCTGAAATGC AGCCTGAGGA TGGAGCTCTT CCTAAGAAGA AGACAAAATC CAAACTTAAG 
ATTCCTTGCA TACGATTCTC AAGAGGGGCA AAGAGAAGTC GTCCTTCCAA ACTCACAGAA 
GATTCAGGCT ATGTCCGAGT CCAGGGAGAG GCAGACGATT TGGAGATAAA AGCCCAGATC 
CAGCCCGATG AGCAGGCAAC CCAGGCTAAG TCCACCCAGG GCCTACAGGA AGATGTGATG 
GTGAGAGATG GTAAGGAGAT CCAAGAATCA CACATAAGCA ACAACGTCAT ATCGGGAGAG 
CATGTGATTG CCATAGAACT GGAATTAGAA AAGGAATCTT CTGCTCTCCG GATGAGAACT 
CCAGGGAGTG AAAAGGAGGC TAAAGTGATT CTGGTAAAGC AGAGTGTTCA GGTACAGCAA 
GCAAGTGTGC TTGAGAATTC AGCAGCAGAC AGCCCTCAGC CAGTGACTTC TACTGCTCCT 
CTCTCACCAG CAACCACACA TCAACTCGGT CTGGAAGAAC CCAGTGACAG CATCCGGGAA 
AGTGCACCAA GTGGAAAAGA TGACGGAAGG AGAAAGACTG CTGCTGAAGA AAAGAAATCA 
GGAGAGACTG CGCTGGGCCA GGCAGAGGAG GCTTCTTCAG TCAGCCAGGC AGACAAAAGT 
GTACTAAGCC AGGCAGAAGA AGCTACAGTG GGCCACACAG AGGAAGCTAC TGTGATCCAG 
GCACAGAGTC AGGCAAAGGA AGGTAAACTG AGCCAGGCAG AGGAAGCTAC AGTGGCCCAG 
GCAAAGGAAA CTGTATTGAG TCAGGCAGAG GAAGTTAAAC TGAGCCAGAT AGAAGAACCT 
GCAATTAGCC AGGCAAAGAA AGCTACAGTA GGACAAGCAA AGGAAGCTAT AGTTAGCCAG 
GCAGAGGAAG CTATAGTTGG TCACACAGAG AAAGCCACCA TGGGTCAGGC AGAGGAAGCT 
ACAGTTGGCC ACATAGAGAA AACCACAGTG GGCCAGGCAG AGGAAGCTAC AGTGGGTCAA 
GCAGAGGAAG CCACAGTGGG TCAAGCAGAG GAAGCCACAG TGGGTCAAGC AGAGGAAGCT 
ACAGTTGGAC AGGCAGAGGA AGCTACAGTT GGTCAGGCAG GGGAAGCTAC AGTTAGCCAC 
ATAGAGAAAA CCACAGTGGG CCAGGCAGAG GAAGCTATAG TTGGTCAGGC AGAGGAAGCC 
ACAGTGGGTC AAGCAGAGGA AGCCACAGTG GGTCAAGCAG AGGAAGCTAC AGTTGGCCAG 
GCAGAGGAAG CCACAGTGGA TCAAGCAGAG GAAGCTACAG TTGGCCAGGC AGAGGAAGCT 
ACAGTTGGTC AGGCAGGGGA AGCTGCAGTT GGCCAGGCAG AGGAAGCTAT AGTTGCTCAG 
GCAGAGGAAG CCACAGTTGG TCAGGCAGGG GAAGCTACAG TTGGCCAAGC AGAGAAAGCC 
ACAGTTGGCC AAGCAGAGGA ACCCATAGTT GGCCAGGCAG AGGAAACTGT ATTGAGGCAT 
GCATCAGATC TGAAAGTAAA TGGAGTTGAT GCAGAGAAAC CAAGATCAGA AGAAAGCAAA 
AGAATGGAGC CAATTGCTAT TATCATAACA GACACTGAAA TCAGTGAATT TGATGTTAAG 
AAATCTAAAA ATGTCCCTAA GCAATTCCTA ATTTCCATGG AAAATGAGCA AGTGGGGGTT 
TTTGCTAATG ATAGTGATTT CGAGGGGAGA ACTTCAGAAC AGTATGAAAC ACTCTTAATA 
GAAACAGCTT CTTCTCTCGT CAAGAATGCT ATCGAGTTGT CTGTAGAACA GCTGGTTAAC 
GAAATGGTTT CTGAGGATAA TCAAATAAAT ACGCTGTTCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of plasma membrane-associated AKAPs for the regulation of cardiac I
Naunyn-Schmiedeberg's archives of pharmacology390(5)493-503(2017 May)
Seyler C,Scherer D,K?pple C,Kulzer M,Korkmaz S,Xynogalos P,Thomas D,Kaya Z,Scholz E,Backs J,Karle C,Katus HA,Zitron E

Peripheral group I metabotropic glutamate receptor activation leads to muscle mechanical hyperalgesia through TRPV1 phosphorylation in the rat.
The journal of pain : official journal of the American Pain Society16(1)67-76(2015 Jan)
Chung MK,Lee J,Joseph J,Saloman J,Ro JY

PKA-GluA1 coupling via AKAP5 controls AMPA receptor phosphorylation and cell-surface targeting during bidirectional homeostatic plasticity.
Neuron84(4)790-805(2014 Nov)
Diering GH,Gustina AS,Huganir RL

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Akap5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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