AKAP5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKAP5
Entrez Gene ID	9495
Full Name	A-kinase anchoring protein 5
Synonyms	AKAP75,AKAP79,H21
General protein information	Preferred Names A-kinase anchoring protein 5 Names A-kinase anchor protein 5 A kinase (PRKA) anchor protein 5 A-kinase anchor protein 79 kDa A-kinase anchoring protein 75/79 AKAP 79 cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q23.3
Summary	The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008].
Disorder MIM:	604688

mRNA and Protein(s)

mRNA	Protein	Name
NM_004857.3	NP_004848.3	A-kinase anchor protein 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Akap5	NP_598199.2
Bos taurus (cattle)	AKAP5	NP_776661.1
Homo sapiens (human)	AKAP5	NP_004848.3
Pan troglodytes (chimpanzee)	AKAP5	XP_001170633.1
	AKAP5	XP_001101989.1
Canis lupus familiaris (dog)	AKAP5	XP_005623550.1
Mus musculus (house mouse)	Akap5	NP_001094941.1

Gene Ontology
Bibliography

Related articles in PubMed

CaMKII regulates the de-palmitoylation and synaptic removal of AKAP79/150 to mediate structural LTD.
Woolfrey KM, O'Leary H, Goodell DJ, Robertson HR, Horne EA, Coultrap SJ, Dell'Acqua ML, Bayer KU
The Journal of biological chemistry(2017 Dec)

Molecular basis of AKAP79 regulation by calmodulin.
Patel N, Stengel F, Aebersold R, Gold MG
Nature communications8(1)1681(2017 Nov)

Intrinsic disorder within AKAP79 fine-tunes anchored phosphatase activity toward substrates and drug sensitivity.
Nygren PJ, Mehta S, Schweppe DK, Langeberg LK, Whiting JL, Weisbrod CR, Bruce JE, Zhang J, Veesler D, Scott JD
eLife6(2017 Oct)

Regulation of the phosphatase PP2B by protein-protein interactions.
Nygren PJ, Scott JD
Biochemical Society transactions44(5)1313-1319(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate the importance of the AKAP79/PP2B/protein kinase A complex's role in synaptic long-term depression in the CA1 region of the hippocampus.
Title: Regulation of the phosphatase PP2B by protein-protein interactions.

human AKAP79-anchored PKC selectively phosphorylates the Robo3.1 receptor subtype on serine 1330
Title: A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors.

Cigarette smoke-induced changes in AKAP5 and AKAP12 in patients with COPD may affect efficacy of pharmacotherapy.
Title: A-kinase-anchoring proteins coordinate inflammatory responses to cigarette smoke in airway smooth muscle.

GPR30 interacted with membrane-associated guanylate kinases and protein kinase A-anchoring protein (AKAP) 5 in the plasma membrane in a PDZ-dependent manner.
Title: G protein-coupled receptor 30 (GPR30) forms a plasma membrane complex with membrane-associated guanylate kinases (MAGUKs) and protein kinase A-anchoring protein 5 (AKAP5) that constitutively inhibits cAMP production.

a significant role for the AKAP5 scaffold in signaling and trafficking of the beta1-AR in cardiac myocytes and mammalian cells.
Title: Role of AKAP79/150 protein in ?1-adrenergic receptor trafficking and signaling in mammalian cells.

The following AKAP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu18932	NM_004857.3 Latest version!	Homo sapiens A-kinase anchoring protein 5 (AKAP5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKAP5 ( NM_004857.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004848.3 Homo sapiens A-kinase anchoring protein 5 (AKAP5), mRNA.

Price & Availability↑

CloneID	OHu18932
Clone ID Related Accession (Same CDS sequence)	NM_004857.3
Accession Version	NM_004857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA942424.1, BC057229.1, AL122035.6 and BC131516.1. On Oct 12, 2007 this sequence version replaced NM_004857.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315336.1, SRR1660809.28018.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394718.4/ ENSP00000378207.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAACCA CAATTTCAGA AATTCATGTA GAAAACAAGG ATGAGAAGAG ATCAGCAGAA 
GGTAGTCCTG GGGCTGAAAG GCAGAAGGAA AAGGCATCCA TGCTTTGCTT CAAGAGAAGA 
AAGAAAGCAG CCAAAGCACT GAAGCCCAAA GCTGGCTCTG AAGCTGCTGA TGTGGCAAGG 
AAGTGTCCAC AAGAAGCAGG AGCTTCTGAT CAGCCAGAGC CCACACGGGG GGCCTGGGCC 
TCACTCAAAC GTCTTGTAAC ACGCAGGAAA AGGTCAGAGT CTTCAAAGCA GCAAAAGCCA 
TTGGAGGGTG AAATGCAACC TGCAATAAAT GCTGAGGATG CTGATCTTTC TAAGAAAAAG 
GCAAAATCTA GACTTAAGAT TCCCTGCATA AAATTCCCAA GAGGGCCAAA AAGGAGTAAT 
CATTCCAAAA TTATAGAAGA CTCAGACTGC AGCATCAAAG TCCAGGAAGA AGCTGAAATT 
TTGGATATAC AAACACAGAC CCCATTGAAT GATCAGGCAA CAAAGGCTAA GTCAACCCAG 
GATCTAAGTG AAGGCATCTC ACGGAAAGAT GGTGATGAGG TCTGTGAATC AAATGTGAGC 
AATAGCACAA CTTCTGGAGA GAAAGTGATT TCAGTAGAAC TTGGATTAGA TAATGGGCAT 
TCTGCTATTC AAACGGGAAC TCTAATCCTT GAAGAAATTG AAACGATCAA GGAAAAACAA 
GATGTTCAAC CCCAGCAAGC AAGCCCACTT GAAACTTCAG AAACAGACCA TCAGCAGCCA 
GTACTTTCTG ATGTTCCTCC TTTACCTGCA ATTCCAGATC AACAAATTGT GGAAGAAGCC 
AGTAACAGTA CCCTAGAAAG TGCACCAAAT GGAAAAGACT ATGAAAGTAC AGAGATTGTA 
GCTGAAGAAA CTAAGCCAAA AGATACTGAA TTGAGCCAAG AATCAGATTT TAAAGAAAAT 
GGGATCACTG AAGAGAAATC CAAATCAGAA GAAAGCAAAA GAATGGAGCC AATTGCTATT 
ATTATTACAG ACACTGAAAT CAGTGAATTT GATGTTACAA AATCTAAAAA TGTCCCTAAG 
CAATTCTTAA TTTCAGCTGA AAATGAGCAA GTAGGGGTTT TTGCTAATGA TAATGGTTTT 
GAGGATAGAA CTTCAGAACA ATATGAAACA CTCTTAATTG AAACAGCCTC TTCTCTAGTC 
AAGAATGCTA TTCAGTTGTC AATAGAACAG CTGGTTAATG AAATGGCCTC TGATGATAAT 
AAAATAAACA ATCTTCTACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004848.3
CDS	379..1662
Translation	METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEASNSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAIIITDTEISEFDVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSLVKNAIQLSIEQLVNEMASDDNKINNLLQ

Target ORF information:

RefSeq Version	NM_004857.3
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 5 (AKAP5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004857.3

ORF Insert Sequence:

ATGGAAACCA CAATTTCAGA AATTCATGTA GAAAACAAGG ATGAGAAGAG ATCAGCAGAA 
GGTAGTCCTG GGGCTGAAAG GCAGAAGGAA AAGGCATCCA TGCTTTGCTT CAAGAGAAGA 
AAGAAAGCAG CCAAAGCACT GAAGCCCAAA GCTGGCTCTG AAGCTGCTGA TGTGGCAAGG 
AAGTGTCCAC AAGAAGCAGG AGCTTCTGAT CAGCCAGAGC CCACACGGGG GGCCTGGGCC 
TCACTCAAAC GTCTTGTAAC ACGCAGGAAA AGGTCAGAGT CTTCAAAGCA GCAAAAGCCA 
TTGGAGGGTG AAATGCAACC TGCAATAAAT GCTGAGGATG CTGATCTTTC TAAGAAAAAG 
GCAAAATCTA GACTTAAGAT TCCCTGCATA AAATTCCCAA GAGGGCCAAA AAGGAGTAAT 
CATTCCAAAA TTATAGAAGA CTCAGACTGC AGCATCAAAG TCCAGGAAGA AGCTGAAATT 
TTGGATATAC AAACACAGAC CCCATTGAAT GATCAGGCAA CAAAGGCTAA GTCAACCCAG 
GATCTAAGTG AAGGCATCTC ACGGAAAGAT GGTGATGAGG TCTGTGAATC AAATGTGAGC 
AATAGCACAA CTTCTGGAGA GAAAGTGATT TCAGTAGAAC TTGGATTAGA TAATGGGCAT 
TCTGCTATTC AAACGGGAAC TCTAATCCTT GAAGAAATTG AAACGATCAA GGAAAAACAA 
GATGTTCAAC CCCAGCAAGC AAGCCCACTT GAAACTTCAG AAACAGACCA TCAGCAGCCA 
GTACTTTCTG ATGTTCCTCC TTTACCTGCA ATTCCAGATC AACAAATTGT GGAAGAAGCC 
AGTAACAGTA CCCTAGAAAG TGCACCAAAT GGAAAAGACT ATGAAAGTAC AGAGATTGTA 
GCTGAAGAAA CTAAGCCAAA AGATACTGAA TTGAGCCAAG AATCAGATTT TAAAGAAAAT 
GGGATCACTG AAGAGAAATC CAAATCAGAA GAAAGCAAAA GAATGGAGCC AATTGCTATT 
ATTATTACAG ACACTGAAAT CAGTGAATTT GATGTTACAA AATCTAAAAA TGTCCCTAAG 
CAATTCTTAA TTTCAGCTGA AAATGAGCAA GTAGGGGTTT TTGCTAATGA TAATGGTTTT 
GAGGATAGAA CTTCAGAACA ATATGAAACA CTCTTAATTG AAACAGCCTC TTCTCTAGTC 
AAGAATGCTA TTCAGTTGTC AATAGAACAG CTGGTTAATG AAATGGCCTC TGATGATAAT 
AAAATAAACA ATCTTCTACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CaMKII regulates the de-palmitoylation and synaptic removal of AKAP79/150 to mediate structural LTD.
The Journal of biological chemistry(2017 Dec)
Woolfrey KM,O'Leary H,Goodell DJ,Robertson HR,Horne EA,Coultrap SJ,Dell'Acqua ML,Bayer KU

Molecular basis of AKAP79 regulation by calmodulin.
Nature communications8(1)1681(2017 Nov)
Patel N,Stengel F,Aebersold R,Gold MG

Intrinsic disorder within AKAP79 fine-tunes anchored phosphatase activity toward substrates and drug sensitivity.
eLife6(2017 Oct)
Nygren PJ,Mehta S,Schweppe DK,Langeberg LK,Whiting JL,Weisbrod CR,Bruce JE,Zhang J,Veesler D,Scott JD

Regulation of the phosphatase PP2B by protein-protein interactions.
Biochemical Society transactions44(5)1313-1319(2016 10)
Nygren PJ,Scott JD

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AKAP5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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