Fgf21 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fgf21
Entrez Gene ID	170580
Full Name	fibroblast growth factor 21
General protein information	Preferred Names fibroblast growth factor 21 Names fibroblast growth factor 21
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	member of the fibroblast growth factor (FGF) family; implicated in activities including embryonic development, cell growth, morphogenesis, tissue growth, and tumor growth and invasion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130752.1	NP_570108.1	fibroblast growth factor 21 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FGF21	XP_524333.1
Canis lupus familiaris (dog)	FGF21	XP_541510.2
Bos taurus (cattle)	FGF21	XP_002695246.1
Mus musculus (house mouse)	Fgf21	NP_064397.1
Rattus norvegicus (Norway rat)	Fgf21	NP_570108.1
Homo sapiens (human)	FGF21	NP_061986.1

Gene Ontology
Bibliography

Related articles in PubMed

Dietary Manipulations That Induce Ketosis Activate the HPA Axis in Male Rats and Mice: A Potential Role for Fibroblast Growth Factor-21.
Ryan KK, Packard AEB, Larson KR, Stout J, Fourman SM, Thompson AMK, Ludwick K, Habegger KM, Stemmer K, Itoh N, Perez-Tilve D, Tsch?p MH, Seeley RJ, Ulrich-Lai YM
Endocrinology159(1)400-413(2018 Jan)

Fibroblast growth factor 21 ameliorates vascular calcification by inhibiting osteogenic transition in vitamin D3 plus nicotine-treated rats.
Shi Y, Lu W, Hou Y, Fu K, Gan F, Liu J
Biochemical and biophysical research communications495(4)2448-2455(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FGF21 inhibited VSMCs calcification via OPG/RANKL system, and through P38 andPI3K/AKT pathways.
Title: Fibroblast growth factor 21 plays an inhibitory role in vascular calcification in?vitro through OPG/RANKL system.

A significant increase in hepatic fibroblast growth factor 21 (FGF21) protein expression was observed in high-fat high-fructose diet (HFFD)-fed males but not in HFFD-fed females, suggesting a sex difference in diet induced fatty liver.
Title: Hepatic FGF21 mediates sex differences in high-fat high-fructose diet-induced fatty liver.

central FGF21 administration induced a significant increase in food intake, possibly mediated by increased NPY and AgRP, and decreased POMC and CART gene expression.
Title: Effects of central fibroblast growth factor 21 (FGF21) in energy balance.

in the present study we demonstrated behavioral effects induced by central FGF-21 and irisin injections that could involve increased DA signaling in the prefrontal cortex
Title: Effects of central fibroblast growth factor 21 and irisin in anxiety-like behavior.

glucagon plus insulin increases FGF21 transcription by stimulating ATF4 expr
Title: Glucagon and Insulin Cooperatively Stimulate Fibroblast Growth Factor 21 Gene Transcription by Increasing the Expression of Activating Transcription Factor 4.

The following Fgf21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fgf21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00457	NM_130752.1 Latest version!	Rattus norvegicus fibroblast growth factor 21 (Fgf21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fgf21 ( NM_130752.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_570108.1 Rattus norvegicus fibroblast growth factor 21 (Fgf21), mRNA.

Price & Availability↑

CloneID	ORa00457
Clone ID Related Accession (Same CDS sequence)	NM_130752.1
Accession Version	NM_130752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Rattus norvegicus(Norway rat)
Product	fibroblast growth factor 21 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB078901.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB078901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACTGGA TGAAATCTAG AGTTGGGGCC CCGGGACTGT GGGTCTGTCT CCTGCTGCCT 
GTCTTCCTGC TGGGGGTGTG CGAGGCATAC CCCATCTCTG ACTCCAGCCC CCTCCTCCAG 
TTTGGGGGTC AAGTCCGACA GAGGTATCTC TACACAGATG ACGACCAGGA CACCGAAGCC 
CACCTGGAGA TCAGGGAGGA CGGAACAGTG GTGGGCACAG CACACCGCAG TCCAGAAAGT 
CTCCTGGAGC TCAAAGCCTT GAAGCCAGGG GTCATTCAAA TCCTGGGTGT CAAAGCCTCT 
AGGTTTCTTT GCCAACAACC AGATGGAACT CTCTATGGAT CGCCTCACTT TGATCCTGAG 
GCCTGCAGTT TCAGAGAGCT GCTGCTTAAG GACGGATACA ATGTGTACCA GTCTGAGGCC 
CATGGCCTGC CCCTGCGTCT GCCCCAGAAG GACTCCCAGG ATCCAGCAAC CCGGGGACCT 
GTGCGCTTCC TGCCCATGCC AGGCCTGCCC CACGAGCCCC AAGAGCAACC AGGAGTCCTT 
CCCCCAGAGC CCCCAGATGT GGGTTCCTCC GACCCCCTGA GCATGGTAGA GCCTTTGCAA 
GGCCGAAGCC CCAGCTATGC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570108.1
CDS	1..627
Translation	MDWMKSRVGAPGLWVCLLLPVFLLGVCEAYPISDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGTAHRSPESLLELKALKPGVIQILGVKASRFLCQQPDGTLYGSPHFDPEACSFRELLLKDGYNVYQSEAHGLPLRLPQKDSQDPATRGPVRFLPMPGLPHEPQEQPGVLPPEPPDVGSSDPLSMVEPLQGRSPSYAS

Target ORF information:

RefSeq Version	NM_130752.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fibroblast growth factor 21 (Fgf21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130752.1

ORF Insert Sequence:

ATGGACTGGA TGAAATCTAG AGTTGGGGCC CCGGGACTGT GGGTCTGTCT CCTGCTGCCT 
GTCTTCCTGC TGGGGGTGTG CGAGGCATAC CCCATCTCTG ACTCCAGCCC CCTCCTCCAG 
TTTGGGGGTC AAGTCCGACA GAGGTATCTC TACACAGATG ACGACCAGGA CACCGAAGCC 
CACCTGGAGA TCAGGGAGGA CGGAACAGTG GTGGGCACAG CACACCGCAG TCCAGAAAGT 
CTCCTGGAGC TCAAAGCCTT GAAGCCAGGG GTCATTCAAA TCCTGGGTGT CAAAGCCTCT 
AGGTTTCTTT GCCAACAACC AGATGGAACT CTCTATGGAT CGCCTCACTT TGATCCTGAG 
GCCTGCAGTT TCAGAGAGCT GCTGCTTAAG GACGGATACA ATGTGTACCA GTCTGAGGCC 
CATGGCCTGC CCCTGCGTCT GCCCCAGAAG GACTCCCAGG ATCCAGCAAC CCGGGGACCT 
GTGCGCTTCC TGCCCATGCC AGGCCTGCCC CACGAGCCCC AAGAGCAACC AGGAGTCCTT 
CCCCCAGAGC CCCCAGATGT GGGTTCCTCC GACCCCCTGA GCATGGTAGA GCCTTTGCAA 
GGCCGAAGCC CCAGCTATGC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dietary Manipulations That Induce Ketosis Activate the HPA Axis in Male Rats and Mice: A Potential Role for Fibroblast Growth Factor-21.
Endocrinology159(1)400-413(2018 Jan)
Ryan KK,Packard AEB,Larson KR,Stout J,Fourman SM,Thompson AMK,Ludwick K,Habegger KM,Stemmer K,Itoh N,Perez-Tilve D,Tsch?p MH,Seeley RJ,Ulrich-Lai YM

Fibroblast growth factor 21 ameliorates vascular calcification by inhibiting osteogenic transition in vitamin D3 plus nicotine-treated rats.
Biochemical and biophysical research communications495(4)2448-2455(2018 Jan)
Shi Y,Lu W,Hou Y,Fu K,Gan F,Liu J

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Fgf21 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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